LOCUS       DACEKG010000001        68781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEKG010000001 DACEKG010000000
VERSION     DACEKG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.001"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            233..475
                     /locus_tag="IW664_000001"
     CDS             233..475
                     /locus_tag="IW664_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0847402.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            472..942
                     /locus_tag="IW664_000002"
     CDS             472..942
                     /locus_tag="IW664_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0847403.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1070..2284
                     /locus_tag="IW664_000003"
     CDS             1070..2284
                     /locus_tag="IW664_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0847404.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2994..3182)
                     /locus_tag="IW664_000004"
     CDS             complement(2994..3182)
                     /locus_tag="IW664_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847405.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3273..3482
                     /locus_tag="IW664_000005"
     CDS             3273..3482
                     /locus_tag="IW664_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0847406.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3594..5207
                     /locus_tag="IW664_000006"
     CDS             3594..5207
                     /locus_tag="IW664_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0847407.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5242..6453
                     /locus_tag="IW664_000007"
                     /pseudo
     CDS             5242..6453
                     /locus_tag="IW664_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6476..7402
                     /gene="glsA"
                     /locus_tag="IW664_000008"
     CDS             6476..7402
                     /gene="glsA"
                     /locus_tag="IW664_000008"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0847408.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7610..9070
                     /locus_tag="IW664_000009"
     CDS             7610..9070
                     /locus_tag="IW664_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847409.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9291..11054
                     /locus_tag="IW664_000010"
     CDS             9291..11054
                     /locus_tag="IW664_000010"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0847410.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11138..11596
                     /locus_tag="IW664_000011"
     CDS             11138..11596
                     /locus_tag="IW664_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0847411.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11705..12388
                     /locus_tag="IW664_000012"
     CDS             11705..12388
                     /locus_tag="IW664_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0847412.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12535..13149
                     /locus_tag="IW664_000013"
     CDS             12535..13149
                     /locus_tag="IW664_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0847413.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13165..13371
                     /locus_tag="IW664_000014"
     CDS             13165..13371
                     /locus_tag="IW664_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847414.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13912..15042
                     /locus_tag="IW664_000015"
     CDS             13912..15042
                     /locus_tag="IW664_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0847415.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15134..15889
                     /locus_tag="IW664_000016"
     CDS             15134..15889
                     /locus_tag="IW664_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0847416.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15947..17986
                     /locus_tag="IW664_000017"
     CDS             15947..17986
                     /locus_tag="IW664_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0847417.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18095..19246)
                     /locus_tag="IW664_000018"
     CDS             complement(18095..19246)
                     /locus_tag="IW664_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0847418.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19400..19930)
                     /locus_tag="IW664_000019"
     CDS             complement(19400..19930)
                     /locus_tag="IW664_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847419.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20029..20379)
                     /locus_tag="IW664_000020"
     CDS             complement(20029..20379)
                     /locus_tag="IW664_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0847420.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20536..22065)
                     /gene="ahpF"
                     /locus_tag="IW664_000021"
     CDS             complement(20536..22065)
                     /gene="ahpF"
                     /locus_tag="IW664_000021"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0847421.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMVMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22075..22638)
                     /gene="ahpC"
                     /locus_tag="IW664_000022"
     CDS             complement(22075..22638)
                     /gene="ahpC"
                     /locus_tag="IW664_000022"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0847422.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22782..23444)
                     /locus_tag="IW664_000023"
     CDS             complement(22782..23444)
                     /locus_tag="IW664_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0847423.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23628..25274
                     /locus_tag="IW664_000024"
     CDS             23628..25274
                     /locus_tag="IW664_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0847424.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25265..26347
                     /gene="uxuA"
                     /locus_tag="IW664_000025"
     CDS             25265..26347
                     /gene="uxuA"
                     /locus_tag="IW664_000025"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0847425.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26455..28008)
                     /locus_tag="IW664_000026"
                     /pseudo
     CDS             complement(26455..28008)
                     /locus_tag="IW664_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            28156..29297
                     /locus_tag="IW664_000027"
                     /pseudo
     CDS             28156..29297
                     /locus_tag="IW664_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29423..30502)
                     /locus_tag="IW664_000028"
     CDS             complement(29423..30502)
                     /locus_tag="IW664_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0847426.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(30605..31045)
                     /locus_tag="IW664_000029"
     CDS             complement(30605..31045)
                     /locus_tag="IW664_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0847427.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31102..31512)
                     /locus_tag="IW664_000030"
     CDS             complement(31102..31512)
                     /locus_tag="IW664_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0847428.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(31692..32126)
                     /locus_tag="IW664_000031"
     CDS             complement(31692..32126)
                     /locus_tag="IW664_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0847429.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32226..33074)
                     /locus_tag="IW664_000032"
     CDS             complement(32226..33074)
                     /locus_tag="IW664_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0847430.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            33463..35229
                     /locus_tag="IW664_000033"
     CDS             33463..35229
                     /locus_tag="IW664_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0847431.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35331..36590
                     /locus_tag="IW664_000034"
     CDS             35331..36590
                     /locus_tag="IW664_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0847432.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            36737..38038
                     /locus_tag="IW664_000035"
     CDS             36737..38038
                     /locus_tag="IW664_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0847433.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38041..38892
                     /locus_tag="IW664_000036"
     CDS             38041..38892
                     /locus_tag="IW664_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0847434.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38905..39720
                     /locus_tag="IW664_000037"
     CDS             38905..39720
                     /locus_tag="IW664_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0847435.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39871..41037
                     /locus_tag="IW664_000038"
     CDS             39871..41037
                     /locus_tag="IW664_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0847436.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            41267..41914
                     /locus_tag="IW664_000039"
     CDS             41267..41914
                     /locus_tag="IW664_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0847437.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            41998..42942
                     /locus_tag="IW664_000040"
     CDS             41998..42942
                     /locus_tag="IW664_000040"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0847438.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            43077..44528
                     /locus_tag="IW664_000041"
     CDS             43077..44528
                     /locus_tag="IW664_000041"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0847439.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            44549..45385
                     /locus_tag="IW664_000042"
     CDS             44549..45385
                     /locus_tag="IW664_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0847440.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            45385..46044
                     /locus_tag="IW664_000043"
     CDS             45385..46044
                     /locus_tag="IW664_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0847441.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            46170..46859
                     /locus_tag="IW664_000044"
     CDS             46170..46859
                     /locus_tag="IW664_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847442.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            46865..47821
                     /locus_tag="IW664_000045"
     CDS             46865..47821
                     /locus_tag="IW664_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0847443.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            47865..48377
                     /locus_tag="IW664_000046"
     CDS             47865..48377
                     /locus_tag="IW664_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0847444.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(48633..49181)
                     /locus_tag="IW664_000047"
     CDS             complement(48633..49181)
                     /locus_tag="IW664_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0847445.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(49270..50451)
                     /locus_tag="IW664_000048"
     CDS             complement(49270..50451)
                     /locus_tag="IW664_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0847446.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(50687..52195)
                     /locus_tag="IW664_000049"
     CDS             complement(50687..52195)
                     /locus_tag="IW664_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0847447.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            52354..52545
                     /locus_tag="IW664_000050"
     CDS             52354..52545
                     /locus_tag="IW664_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847448.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            52748..53665
                     /locus_tag="IW664_000051"
     CDS             52748..53665
                     /locus_tag="IW664_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0847449.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            53658..54731
                     /locus_tag="IW664_000052"
     CDS             53658..54731
                     /locus_tag="IW664_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847450.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            54879..55397
                     /locus_tag="IW664_000053"
     CDS             54879..55397
                     /locus_tag="IW664_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847451.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            55378..55770
                     /locus_tag="IW664_000054"
     CDS             55378..55770
                     /locus_tag="IW664_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0847452.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            55846..56781
                     /locus_tag="IW664_000055"
     CDS             55846..56781
                     /locus_tag="IW664_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0847453.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            56914..57693
                     /locus_tag="IW664_000056"
     CDS             56914..57693
                     /locus_tag="IW664_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0847454.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            57686..58003
                     /locus_tag="IW664_000057"
     CDS             57686..58003
                     /locus_tag="IW664_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0847455.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            58247..59857
                     /locus_tag="IW664_000058"
     CDS             58247..59857
                     /locus_tag="IW664_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0847456.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            60014..60862
                     /locus_tag="IW664_000059"
     CDS             60014..60862
                     /locus_tag="IW664_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0847457.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            61093..61572
                     /locus_tag="IW664_000060"
     CDS             61093..61572
                     /locus_tag="IW664_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847458.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            61605..63833
                     /locus_tag="IW664_000061"
     CDS             61605..63833
                     /locus_tag="IW664_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0847459.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            63962..65605
                     /locus_tag="IW664_000062"
     CDS             63962..65605
                     /locus_tag="IW664_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0847460.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(65678..66223)
                     /locus_tag="IW664_000063"
     CDS             complement(65678..66223)
                     /locus_tag="IW664_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0847461.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(66246..66731)
                     /locus_tag="IW664_000064"
     CDS             complement(66246..66731)
                     /locus_tag="IW664_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0847462.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(66721..67533)
                     /locus_tag="IW664_000065"
     CDS             complement(66721..67533)
                     /locus_tag="IW664_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0847463.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(67672..>68781)
                     /locus_tag="IW664_000066"
     CDS             complement(67672..>68781)
                     /locus_tag="IW664_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0847464.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        23017 a        12149 c        13517 g        20098 t
ORIGIN      
        1 agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat ggtagttgtc
       61 gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg gttggagatg
      121 ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga gaatgttata
      181 attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa atatggaaaa
      241 tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta ttagaattaa
      301 aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa aaattagcct
      361 ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg ataattgtat
      421 tataacggta aatgcgtaca gttataccgt tatcactgct cacaaggtaa aatgataaat
      481 ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga actgactaat
      541 tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa cagtagtttg
      601 aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat ggaaagaaga
      661 aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc agagggcttt
      721 ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg tgctatggat
      781 aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac atcgtatatt
      841 cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca ttggaatagt
      901 gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt ttatatccat
      961 acacacaaag cagttagtct taggattgac tgctttttct atacagattt ttaatgacag
     1021 gcaattatca attatggtag ttgccttttt tgattggagg aattgaagaa tgaatgataa
     1081 aaactttatt gaagaactaa gacaaaagcg tgaggaatac ggagtaacac aaacaaggct
     1141 tgctgttgcc tgtggtatca gccgtgaata ttacaaccgc attgaaaaag gaaaacagcc
     1201 attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca atccgcaaga
     1261 accactattc ttattgattg attactttcg tgtccgcttt cctactacgg acgcattggc
     1321 gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag attatggaaa
     1381 atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt ccatgcagga
     1441 gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg aatgttatct
     1501 gctggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg ctggtggtgt
     1561 gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata ttccaaagtt
     1621 aaaggaaaaa tacaaggctg gcgaatgtat gtcctacttc cgtatgcaga aagattacag
     1681 cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct tatatctcgg
     1741 ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc agtatgtcaa
     1801 gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac gcatgaagaa
     1861 cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg aacgcaccgc
     1921 tttttctatc atcaatcatt atgtccgctt tgttgacaga gaggacgaca agccaaaaag
     1981 tcaatggata acaaatgatg attgggcatg gtttgtaggg gaaaacagag agccgatacg
     2041 cttaaccaca aagccagagc cttacacttt acaaaaagcg ttgcattggc tacaaagaca
     2101 agtcgcacca accataaaaa tggtacaggc attagacaga gaaaatcgta caacgatact
     2161 gaaagatatg attgagcagg cagaactaaa agacaagcat aaacatttat tacaattaga
     2221 gaaatcaacc atagaagaac gtatagatac cgctgttcca caagagaatg acggtatttt
     2281 ttaatgtaat tgaaaaattt cagatttagt cagcaaagag ctgttttcat tccctatgtg
     2341 aagtgaaaga agaaaaataa attttttatt aaatctgcaa caaaatggct acttgcgtac
     2401 agatatggtg cgagaaaagg aaatctaaac tttttttgaa aataggtcat taaatcgggg
     2461 cttgctgtcc ctatatgaag tgaaagaaga aaaaagtttt gaaaaaatga cggaaattcg
     2521 tgattagtgg acagtcttct gtgaaaagag gaaaaacatc atcattttgt attcaaacag
     2581 gcattagcag ttgagtgttg atgtgaaaag atgaaaatac tttgttctca acttaacaat
     2641 ttaaggtttc cgttccctat atggtgcaaa gaaaatattt cattctattg ttggcagtaa
     2701 cggtatggtt tgggtagtta gggtgtgaaa aggaaaatat tcttttttca tcatcaactt
     2761 agcaaaatga cattttctat ccctatatgg tgcatgaaaa gaagttaaaa tttttccatt
     2821 taaacttagc aaatggacgt ttgctgtaca gatagtagtg aaaaaggaga aaatcaaccg
     2881 ttgattgcca aaatgaaccg ttccatttgt actaataatg gaaaaagttt tggattttgg
     2941 ttacgtttct cctcttttcc aacgcgatat ataggaaaga cgattttttt ctttcaatcg
     3001 ttctttgaca actgaataaa gctattcaat acgtttgaca gacagctagc cgttgaaaca
     3061 aatacgccac gacctttccc ctgcaagagc gagcgaaaac ctatttgtgt tcttgtaaaa
     3121 gatttgtcct cttttatcgc catgacctgt actgtcagat aatgatactc ccgtacagcc
     3181 acagcttgag cattaagagc gtcgcacgca atgggtacgg ctacataaga actatgcaga
     3241 ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga ttttacagaa
     3301 agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat ggcaagtgtg
     3361 gcattggcaa cagaggaaag acaaagattg tagtaaaaga gcatttttcc gaaaagggca
     3421 aaacaatgga agaacttcta actgatgtca tgctggaaaa agcaaagcag acaatcgcat
     3481 aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg ttatacttta
     3541 cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg acaatgaaac
     3601 aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa ttacagggcg
     3661 aaagttccag cattaccaca cagagaagta tgttgcgttt atatgcaaaa gaacatcatt
     3721 tgaatgtcat tgatgaatat attgatgacg gctggtcggg aacaaatttt gacagaccga
     3781 gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt gtaacgaaag
     3841 acctttcccg tcttggcaga aattatatta tgacaggaca atatacagaa ttgtatttcc
     3901 ctagtcataa tgtccgctac atagcgattg atgacggtgt agacagcgaa aaaggcgaaa
     3961 gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat acaagccgta
     4021 aagtcaaatc agcatttaag acaaagtttg cggagggggc tcattacggt gcttatgctc
     4081 cgttaggata taagaaacac cctgacatca aaggaaaact gttggttgat gatgaaacaa
     4141 aatggattat tgaaaaaatc ttttccctag cctatcaagg ttacggaagt gcaaaaatca
     4201 caaagcagtt aagagcagaa aaagttccga cagcgtcatg gctaaatttt acaagatacg
     4261 gtacttttgc acatatcttt gagggaaaac ccgaaagtaa gcgttatgag tggacgattg
     4321 ctcatgtcaa ggcgatattg aaaagtgagg tttatatcgg aaacagtgtt cacaatatgc
     4381 agtctacggt atcgttcaag agcaaaaaga aagtgcgtaa gcccgaaagt gaatggtttc
     4441 gagtagaaaa cactcacgag ccgattattg acaaggaagt gttctatcgt gtgcaggagc
     4501 agataaaatc aagacgcaga cagacaaagg aaaaggcaac gccgattttt gcagggcttg
     4561 tcaagtgtgc ggattgtggt tggtctatga gatttggaac gaataagaca aataaaacgc
     4621 cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtaat tgttctatgc
     4681 actacattcg ttatgatgtg ctgtatcaag ccgtattgga acgcttgcag tattgggcta
     4741 aggcagtaca gcaggacgaa gaaaaggtat tgaacaagat acagaaagct ggcaatgcag
     4801 agagaatacg ggaaaagaag aaaaaggcaa gtacattgaa gaaagccgag aacagacaaa
     4861 atgagattga ccgtttattt gcgaaaatgt atgaagatag agcctgtgag aagataacag
     4921 agcgaaattt tgtgatgttg tccagcaagt accaaaaaga acagatagaa ctggaacagc
     4981 agataacaag cctaagagaa gaactaagta aaatggaaca ggatatgata ggtgctgaaa
     5041 agtggattga gttaatcaag gaatattccg taccaaagga actgacagca ccattattaa
     5101 atgccatgat agaaaaaatc ctcatacatg aagcaacaac gaatgaggat aacgaaagaa
     5161 tacaggaaat tgagatatat taccgattta ttggaagagt tgagtaatca acaagagtaa
     5221 tatttttaac taagggaaac cggcggtgtc tatacttggg tcagtcgcac attgggaaaa
     5281 cgattcggtt ttgccgcaat ctttttccaa tggtttgaga taacagttgg ctacttgacc
     5341 atgctgtatt tcttaactgg tgcattatcc tatgcaacgg gtattggtgc tatacaaaac
     5401 aataaatatc tgaaattggc aatcctattg attattttct gggctatttt actctcacag
     5461 ttaaaaggaa ccaaatacac ttcattgatt gctcggatcg gttttgtagc tggtatcttg
     5521 ttgccagcat tagtcttgtt tggcttaggg atccattatg tcgcttcagg cgctccacta
     5581 caaacgatac tgtctatgaa aacattgatt cctgattttt cgaaattgcc tacgctggtt
     5641 gtttttgttt cctttatttt agcatatatg ggcgtagaaa catctgccag tcatgcaaat
     5701 gaaatggaaa accctaaaaa ggattatcca ttagcaatgt ttgtattggt tattctggca
     5761 atcattttag atacttttgg cggattgacc gtcgctacta cagtacctca acatggtttg
     5821 tctcttaata caggggtgat tcaatcgtta gaattcctat taagacacat tgatccaaat
     5881 cttgcatgga ttgcgaaaat tcttgcgatt cttgtttgtt taggtgtcat cggagaaatt
     5941 gcttcttggg taacctctcc ttcaaaagca cttcacgtag cagcagaaga aggattactt
     6001 cctgaatatt ttgcaaaaga aaacacacac ggtgttcctg ctcatttgat gatcgcaaat
     6061 gggatcgttg caacaatctg ggcagccgtc tttacattaa gcggcggggg gaataatctt
     6121 tcatttcttg cagcgatgtc attgactgtt gtaatctacc tgatgttgta ctttatgttt
     6181 ttcgcgggct atttgaagct ggtttttaaa gatgacagtc ttgaaagagc ctatcaggtt
     6241 ccaggcggaa aagttgtaaa agtaatcgtt gcttgtttag gtcttttgac ttcacttttt
     6301 gctttttgta tctcatttgt tccacctgct agtatcgcag ctggacagca tgggatttat
     6361 gaaacgattc tgattgtttc ttatcttatc gtcttagtta ttccatttac gatctacgca
     6421 tgccgtcata aattccagca tgcagaaaaa taaaaaatga aagctggtta ttcaaatgga
     6481 agtaaaagaa ttagaacaat tgattggaaa aaatttccac tattatcaaa caggacaagt
     6541 ggcggattac attccagcat tagctgaagt ggatcccaaa caattaggta tggcgattta
     6601 tgatttggat acaaaacaac tgattgaggg aggagacgcc gatgtgcgct ttgccatcga
     6661 aagcatgtca aaagttcccg tattgttatt agcaatcaaa gacaacggaa tagaaaaagt
     6721 ctttcaaaca atcaacacgg aaccaaccgg ttttgccttc aattctatca tgaatatgga
     6781 aatcaatcat cgcaaacatc caatgaatcc ttttgttaat gcaggagcga ttgcaacaac
     6841 ctcgttgatt gccggtaaag atgcagatga aaaatttgag cgaatcttgt catttatgaa
     6901 agagatctgt gatgatccta atatcacatt gaatgaagaa atctatcatt ctgaatcaag
     6961 aacaggtgat atcaatcgct ctcttgctta ctatatgaaa ggtaatcaaa tgatcgaagg
     7021 agacgtaccg gagatactcg atgcttattt cagacaatgc tccctcaatg tcaccgctac
     7081 aggcatagca aaattagctg ctgtgctggc aaataaagga attgctcctt ggaatggcaa
     7141 acaaatcatt actgaagaaa gtgctacaat cgtcaaatca attatgacaa ctgctggttt
     7201 gtatgatgaa tcaggagagt tttccgtcca tgttggtgta cctgcaaaaa gcggtgtagg
     7261 cggcgggttg atggcagctg tgccaaatcg ttacggcata ggtgtcttca gcccggcact
     7321 agatccattt ggtaacagcg cagctggtat ccaattgttg aaagatgtag tcaaagaact
     7381 agacgctgac atctttgaat aaccattctt gaaagaggct ggggtaaaag tccaaacggc
     7441 tcaaagctac ctacaatgag gctgaaattg tgagcttgtt tgacttttgt cccagtttct
     7501 ttttttattg gtttttgaaa gggcttttct aaataaaaag gaaacaaaaa tacgatttac
     7561 agatatgatt aaataaaatc ataagaaaaa ctggaggcgt gtatcaggaa tgctggaaaa
     7621 ttacatcgag cgaaatatat ttagaaaagt gtatctgtgt gaacaattat ttgaattcca
     7681 agaaatagac atcgaacaaa cagctatttc tctaagggtg actacaccaa cgatccttca
     7741 cgatctagaa agtctagccg agtgtctaga atattgtata aaagaacaag taagagagaa
     7801 gcataagtat aaactcgtat tcaaacatgg tattgctctt tctgagctca cacagtttct
     7861 gtacggtcaa tcttattttt taaagttttt aagttattat tttaatggta tattcacgag
     7921 tacagaactg gctgatcttg aatttatttc tttgagtaaa gtatatacaa tcaaaaaaat
     7981 cgtattggat ttttttaaag aaaatgatta tttgaaaaat aaagaaatca ttatcccaga
     8041 atttgactcg cggaacatct tgctagcttt agtacgttat gtcaattggg aaggctatga
     8101 aaataaaaat ccgtatgtag aagaagcttg taacgagttg attgattatg tagagactca
     8161 tttttttaaa agaagatatt cggaagatga aaagtccttt atcatacgag aaatagagat
     8221 cgcaatcgga agaaaggcat ttccccttgg actgaaagaa gaagataaga aaagcgcaga
     8281 aacgaagccg ttatttcaat tagtctgtca aggattggaa gagcaaaaag gaattctaga
     8341 cttacaagaa gaagatgtct attatatttt ttctttgttc aacagtagaa attataccaa
     8401 tgagaacatg gagctgttaa gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa
     8461 tgacctatca ctgaatgaat tgattttaca gattattagt atcgctgaaa tcgacgatgc
     8521 agaaaatttt attttcaagc aatccttttt acaatttgtc cgtacgttgt gggcagatgg
     8581 acaagtattt ttaccggaaa ggatctatct tctatcagaa aaagaacaac ttctgtacgg
     8641 aaaaattgta gaaatattgg aagagtggaa aaagaaaaat caaatccaac taagatggaa
     8701 tgaaaatttc attcgaaaat tcgtcaaaag tctttctttg atcaacagtg aacaagcggg
     8761 gagacaggaa atagaaattt ttgttgtttc agaaagttca gtcaaacaat ttttttatcg
     8821 aacccaattt catttgtatg cggaagaagg catcgctgaa ataaatccta ttttatttcg
     8881 ttcattagaa gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga
     8941 tgctgctagc tatcaaaatg gattagaaac tgaaaagaca aagatctatc caatctcatt
     9001 aaaaggtgca aacatccatt tgtatcatat tgctcaggaa cttcacctat taaaaaaaca
     9061 tatttcttga tgagacctta attaggagac cttctacaaa gctttttaat tgtctgtaga
     9121 aggtttttat ttgagtcctg attcgtgcga agatctactt atataacaag ctttctctga
     9181 aattttttta aaatgctatt gacttagagt caactctaag tagtagaata gataaagtgt
     9241 aacaaatcta ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag
     9301 tattacatgt attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg
     9361 aaatgaaaca gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag
     9421 gtgggcacgt tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat
     9481 ttgattcacc aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc
     9541 ttttaactgg tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt
     9601 ccaaccaaca tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca
     9661 gcttagcaac aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag
     9721 catttatcgg tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg
     9781 gtgttttaca atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga
     9841 atcatggcag tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca
     9901 ataacctatt taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg
     9961 aaacggtgat ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg
    10021 tccatacgga aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt
    10081 ggcatgtacc atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat
    10141 ttaatatgat catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg
    10201 ccgcagtcaa tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc
    10261 cagatcatta ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt
    10321 tggcttctca gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat
    10381 atgatcaatt atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg
    10441 gcggaacgat ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat
    10501 caagtatccc gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc
    10561 tagattgggc gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg
    10621 tagaacatgg tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata
    10681 atggaggaaa agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag
    10741 taaaagcttt tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag
    10801 aaaatgacgt agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag
    10861 aagatggtag tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt
    10921 caatgaacgt attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc
    10981 agctttatga acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct
    11041 tgaatattca ataattcatt atgctcacca tccagataat tgtttatact agtatctgga
    11101 tggttttttc attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata
    11161 tgtcaaaaag ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc
    11221 atttggggat acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa
    11281 gactggtaca tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt
    11341 tggtcaatta gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat
    11401 aaatgtgacc atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga
    11461 aggggatcgg acattagctt attggcggaa agtccatgct gagtttttta aacatgctta
    11521 taaagaagcg ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga
    11581 agtaattttc ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga
    11641 atagaaaagg gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga
    11701 tattgtggat acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga
    11761 aaacgagccg ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg
    11821 cggagcagtt ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt
    11881 tgatttaccg gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac
    11941 tgcgacagaa aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga
    12001 tgcaacttct cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca
    12061 cgaacacggt cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca
    12121 agaagatggt tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa
    12181 gacgcagaca cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt
    12241 aattatggaa ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc
    12301 tttttcagtc gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt
    12361 cgaagccgca agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa
    12421 agaaaaattg atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc
    12481 attttgtctt caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt
    12541 gaacggagtc cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc
    12601 atccctagac cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca
    12661 cccttcagtt acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag
    12721 agaaaacaag atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt
    12781 atccatatcc ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct
    12841 aatgaggaaa gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca
    12901 gtaccagcat tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag
    12961 atacggaata aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat
    13021 ttaaatgaaa cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct
    13081 gtaagccgtt tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt
    13141 cccgaataaa aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca
    13201 aaagaaatga aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa
    13261 gttccgccat tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata
    13321 gaaatgccag atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt
    13381 ccgaaaagtg aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct
    13441 ggtgcaactg aaagaatttg caacgggatt ctttcaactt atttgttatt tttttctaaa
    13501 aaatattaaa gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag
    13561 gaaaagtcta tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc
    13621 tatacaaaca taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac
    13681 tgttggatta aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa
    13741 aactcggacg atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt
    13801 tctttatatt tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt
    13861 ggattgctgt gtgaggagga gatgcggtga aaaaagatcg attggtggat tttgggtcct
    13921 tatttgactt tgtccatgat cggtctttta gaagtataca gtgccagttc ttatcgttta
    13981 ttgcaagccg atgaaaatac aaaaagtcta ttattgcgtc aactaatttt catatttttg
    14041 agttggagcg tgatcttctt agctcgttca gtcaaactac actatttact tcaccctaaa
    14101 atagcaggat acggtttagc cttatcgatt ttctttttag tattagtaag aatagggata
    14161 ttcggtgtca ctgtcaacgg cgcacaacgt tggatctctc tgtttggcat tcaattccag
    14221 ccttctgaac tggcaaatct ttttttgatt ttttatttaa gctggttttt tcgtgacgga
    14281 aatagtagcc caaaagatct aaaaaaacca ttcctgatta cagtaggtat aacttttctg
    14341 attttatttc agccaaagat tgctggagca ttgatgatcc tttcgattgc gtgggtcata
    14401 ttttgggcag cggcggttcc atttaaaaaa gggatctatc taatcgttac tttttctgca
    14461 ttgctgattg gagcagcagg cggggtatta tatttaggaa ataaaggttg gcttccacag
    14521 atgtttaatc atgcttatga aaggatcgcg actttaagag attcttttat cgatagtcat
    14581 ggtgcgggct atcaaatgac ccattcgttt tatgcactat ataatggtgg catatttggg
    14641 cggggattag gaaatagtat cacaaaaaaa gggtatcttc cagagacaga aacagatttt
    14701 attttttcga ttattacaga agagcttggg ctaatcggtg cattgtgtgt gcttttctta
    14761 ttgttctcat tatgcatgag aatcttttgt ttaagcagtc gctgtaaaaa tcagcaagct
    14821 ggattgtttc ttttaggatt cggaactttg ctattcgttc agacgataat gaacgtggga
    14881 agtatcgcgg ggctgatgcc aatgactggt gtgccattgc catttgttag ctacggagga
    14941 acaagttatc tgatcctttc tttaggaatc ggaatcacgt tgaatatttc ttcaaagata
    15001 caggccgaag agttggcgct ctatcgacct gaaaaacaat aaagagaaac tgagaaactc
    15061 tcagtttgaa ttatcataaa gtacaatctt ttatttttca agcgagaggt tcttttagaa
    15121 agaggaattc gcaatgaaag tatctcaaaa aatcatatta tctattttgt tactgcttac
    15181 attagtagtt ggctattttt cgattgagtt tatccatgga ttttcgtctg caaaacagag
    15241 ttcaactgtg aaaaaggtag agccgaaaag tatccctacc acactaaatg tggctttgat
    15301 tggttcagat gctcgttcga aagacgaaaa cggtcgctcg gattcacttc tggttgcaca
    15361 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
    15421 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
    15481 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
    15541 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
    15601 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
    15661 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
    15721 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
    15781 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
    15841 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
    15901 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
    15961 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
    16021 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
    16081 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
    16141 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
    16201 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
    16261 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
    16321 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
    16381 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
    16441 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
    16501 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
    16561 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
    16621 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
    16681 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
    16741 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
    16801 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
    16861 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
    16921 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
    16981 gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc
    17041 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
    17101 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
    17161 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
    17221 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
    17281 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttatcaag caacgcgtgt
    17341 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
    17401 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
    17461 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
    17521 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
    17581 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
    17641 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
    17701 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
    17761 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
    17821 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
    17881 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
    17941 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
    18001 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
    18061 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
    18121 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
    18181 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
    18241 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
    18301 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
    18361 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
    18421 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
    18481 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
    18541 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
    18601 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
    18661 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
    18721 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
    18781 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
    18841 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
    18901 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
    18961 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
    19021 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
    19081 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
    19141 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
    19201 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
    19261 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
    19321 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
    19381 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
    19441 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
    19501 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
    19561 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
    19621 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
    19681 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
    19741 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
    19801 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
    19861 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
    19921 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
    19981 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
    20041 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
    20101 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
    20161 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
    20221 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
    20281 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
    20341 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
    20401 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
    20461 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
    20521 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
    20581 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
    20641 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
    20701 catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac
    20761 tcctgaaaca gctagtgtat gcatcaccat cgtacggcga tcgatatagg agagagattc
    20821 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
    20881 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
    20941 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
    21001 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
    21061 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
    21121 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
    21181 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
    21241 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
    21301 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
    21361 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
    21421 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
    21481 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
    21541 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
    21601 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
    21661 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
    21721 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
    21781 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
    21841 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
    21901 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
    21961 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
    22021 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
    22081 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
    22141 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
    22201 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
    22261 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
    22321 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
    22381 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
    22441 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
    22501 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
    22561 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
    22621 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
    22681 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
    22741 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
    22801 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
    22861 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
    22921 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
    22981 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
    23041 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
    23101 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
    23161 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
    23221 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
    23281 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
    23341 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
    23401 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
    23461 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
    23521 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
    23581 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
    23641 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
    23701 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
    23761 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
    23821 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
    23881 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
    23941 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
    24001 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
    24061 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
    24121 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata
    24181 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
    24241 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
    24301 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
    24361 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
    24421 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
    24481 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
    24541 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
    24601 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
    24661 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
    24721 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
    24781 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc
    24841 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    24901 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    24961 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    25021 cagacccact attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag
    25081 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    25141 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    25201 caggaccaaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    25261 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    25321 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    25381 agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    25441 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    25501 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    25561 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    25621 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    25681 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga
    25741 acctgagcgc ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    25801 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    25861 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg
    25921 aatcgtaaag aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa
    25981 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    26041 tttagcagaa ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    26101 tttagctaat cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat
    26161 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca
    26221 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    26281 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    26341 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    26401 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    26461 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    26521 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    26581 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    26641 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    26701 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    26761 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    26821 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    26881 ataaaattgt tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat
    26941 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    27001 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    27061 agtacaaagg gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    27121 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    27181 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    27241 atctacagag agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    27301 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    27361 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    27421 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    27481 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    27541 gccattatta gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt
    27601 cattttcgta tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga
    27661 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    27721 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    27781 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    27841 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    27901 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    27961 gctttttttc ttcaagataa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    28021 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    28081 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    28141 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    28201 ttatcgctta ctataaatca gacagtccac acagaggaga cagaaacagc caaatggaca
    28261 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    28321 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    28381 tcgggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccact
    28441 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    28501 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    28561 gatggactac cgaacgatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa
    28621 gattcagcca tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat
    28681 gaaaataagc cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata
    28741 gaacctggag aagaacccga tacagaaact caaccagagc cagacaataa atcaaaacct
    28801 gaaataaccc ctggagaaaa accagatgta actccagaac cagatacgga ttctggaaac
    28861 caaacagtac cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca
    28921 gaggtagatc cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac
    28981 caaggaatac cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt
    29041 aaaaaatcaa atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga
    29101 atatttgata gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct
    29161 gtcctgaaac gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac
    29221 tgtggagcgg aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac
    29281 aaaacagatc tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa
    29341 atatccagaa aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac
    29401 tttatctttc ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga
    29461 tctttttcac tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc
    29521 cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca
    29581 gttctgtttt gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa
    29641 tcaaaatcgt aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga
    29701 aagatagagg aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa
    29761 tcatgatcgc taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga
    29821 gcaacgtatt taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga
    29881 caaacttatc ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa
    29941 acaatcttga gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga
    30001 atgacatagc aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg
    30061 aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt
    30121 gtaaaacagg atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca
    30181 tgtcaaatgt ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt
    30241 aaacgatcag tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct
    30301 gaatgatttg aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg
    30361 taatcatact tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc
    30421 gtattttttc attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac
    30481 aaaaaataaa cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaaga
    30541 aaaaccgcta aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc
    30601 agtttcagcc ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt
    30661 gcagtgtatc caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac
    30721 cttctgcttg tttttgcaac gcagcacctt gagcagtcag cgtaacattt acgattcttt
    30781 catcacccgt ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg
    30841 gagtcagcgt accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt
    30901 tcttttccca taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga
    30961 ggaggggagt gtagtgtcta atgatttctt tagaggcggc atataaagga aaacatactt
    31021 gattaactaa atatagatct tccatttttc cacctccgct gttttactat agtgtatcat
    31081 agcggattta cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc
    31141 agctgtagct tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc
    31201 ttccagagaa agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc
    31261 actttctgaa taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg
    31321 ctgtttgatc aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg
    31381 ttcagggttg gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt
    31441 acgatcggga ttatgcactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata
    31501 catttttttc ataaaattac cacctttaat tatattttat acaattgaat tttacacaat
    31561 ataattgtgg aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa
    31621 cagacgcgct ttctcgaata gcaaacatat acaaaaaata tcagagacaa aaaataatct
    31681 catcataaac attacatata tttctcaaaa agttgttttc tttcttcaaa aattacttca
    31741 cgatggacct gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc
    31801 acaccaaagt ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct
    31861 ttattatgtg tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt
    31921 tcagcagtag gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgta
    31981 ccatacaagg taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt
    32041 tgtatttcat caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca
    32101 aaatcgattt ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac
    32161 tagattcttt tattatacca gctaaagaaa acgcattcaa aaaaatagcg gatttactta
    32221 ccaactcatt cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg
    32281 acaatgacaa aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca
    32341 tgatcagtca caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc
    32401 tctagcaggg taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa
    32461 taatgttcac tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc
    32521 atgacaattg aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa
    32581 ataaacagat taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc
    32641 aagtggaaat attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg
    32701 tttttatata aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac
    32761 aaccgatctg aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaccaag
    32821 cttccgaaaa gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac
    32881 ataacgatat tcaacactaa aataactata gctcggtcaa agcctaaagc agcttccagt
    32941 aatattccta gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg
    33001 atagcgactc ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt
    33061 ctcgtttctt tcatttattc acttccttaa ttcattaata atactcttat cttattataa
    33121 cttacttttc accttaagtt aactttaatt aataaaaaaa ttaggctagt ctaaattatt
    33181 atgtttcatt cttctagaat atacttctac gccaaaaact gttaaaaaac ttttacttta
    33241 gaaaattgga gaaattgatc gcttaagata aattcaggac actaaatagt acaggaagta
    33301 gactaaaact tttaaaagtt ttaagtctat ctatttggca agtttaaaaa ataaaaattc
    33361 ggtatcaaaa aactgttacc gataacattt ttgaaagcgt tgtcttttgc ttctctatca
    33421 cttatagtga tgacgtcata ttaaaacaca aggaggaaat caatggatac agctgcaatt
    33481 tatcatcgac cggaaagtga atatgcattt ctatatacga atacaagatt tcgtattcgg
    33541 cttcgtacta ggaaaaatga tatcaaaaaa gtatatgtgc tatgtggaga tccatatacc
    33601 ataacaactg aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat
    33661 gttcatgatt attgggaaat tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc
    33721 catgttgttg gggaagataa cacggactgt ttttacggtg atcaaggaat ttttccttat
    33781 cgagaaaatg tgattactga accaaatttt tattttcgaa taccttattt tcatcaaatc
    33841 gatcgattca ctattccaga atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa
    33901 agatttgcaa acggggacaa agcaaatgac cccgaacaca ctttaccttg gggaagtaaa
    33961 gatccagacc gagaagactt tttcggggga gatttgcaag gtgtgatgga tcatttggat
    34021 tacttgacgg accttggcat caatggcatc tatttttgtc ccatatttaa agccacatcc
    34081 aatcataaat acgatacgat tgattattat gaggtggatc ccgcttttgg cgataaaaaa
    34141 cttttaaaaa acttgattga tgaagcacat aaaagaggga tacgtatcat gttcgatgct
    34201 gtattcaatc atatgggtgt ccattcacca cagtggcaag acgttctcaa aaacggagaa
    34261 aaatcgatct ataaagactg gttccacatc cgttttttcc cagtagattc ttatcagatg
    34321 actgacttac cagaaactgc tgaaaatatc ccatatgata cttttgcttt tactccattc
    34381 atgccgaaac taaatacagc aaatccagaa gtgcaaaaat atttgctgga tatcgcaact
    34441 tattggataa aagaattcga tattgatgga tggcgtcttg atgttgctaa tgaagtcgat
    34501 catcattttt ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga catctatatc
    34561 cttggagaga tttggcattc ctctcaagct tggctgcaag gggatgaatt ccatgcagtg
    34621 atgaattatg catttacaga ttcgattaaa gattattttg caaaaaagaa aatcacagct
    34681 tcacaaatgg tcagtggaat gaatcatcag caaatgcttt acagagatca ggtgaacgaa
    34741 ggaacattca acttattaga ttctcatgat actgccagga tcttgaccct ttgccaaggt
    34801 aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt
    34861 atctattacg ggacagaaat agggatgact ggtgaagatg acccggattg ccgcaaatgt
    34921 atgatatggg agaaggagga acaagatcta gagctattcg gttttataaa agaacttgtt
    34981 tctctaagga aacagttgtc taagattatt tctgaaggtt ctactcagtg gctaatagta
    35041 aatgatcgtg aagataagct ctatttcaca cggaaactag agggacagat catctatgtt
    35101 tactttaatc agtcaaaaga accttgggta gtagaacagg aaaatgaggt gattcttagt
    35161 caaaattgtc agttgttaga agacgggaaa gcagagatac gacaatacgg atttttgatc
    35221 atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc
    35281 cattataata agggtgtaac agcacgttga atacaaagga ggaaatgaag atgaaaaaaa
    35341 gaagtgtgaa acagttgggt ttagggttaa tctctttagg aatcttaggc ggattagcgg
    35401 cttgtggcaa tggtaactct tcagcagaag gggaagccga tgaaggaaaa acgttaacag
    35461 tttccgttga ttctggatat aaagattata taaacgaaat aaaaaacact ttcgaaaaag
    35521 aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg gaatcattag
    35581 cgttagatgg gcctgctgga aagggacctg acgttatgat ggcagcatat gaccggatcg
    35641 gtgcgttagg acagcaagga catctggcgg aagtaaaact gggaaatgaa gcagcttacg
    35701 atgaaacaga caaagcccaa gtgacttatg atggaaaaat ctacggagaa cctgcagtaa
    35761 tcgagaccct tgttttgtac tataacaaag atttagtcga tacagcacca tcaacgttta
    35821 aagatttgga gaatctatcc aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa
    35881 atacaggatt tttagctaaa tggactgact tttattattc ctatggttta attgcaggtt
    35941 acggtggcta tgtatttggt gatgatggca caaatccttc agatatcggt ttgaataatg
    36001 caggtgctgt agaaggaatc tcttatgcaa cagattggtt caaaaatgtt tggccaaagg
    36061 gaatgcaaga cataaaaagt gcaggagact tcgccagcca gcaattcatg tccaataaaa
    36121 ctgcagcaat cattgacggt ccatggcaag cgcaaacata caaagaaaac aatatcaatt
    36181 atggtgtagc caaaattcct actctaaata atggacagcc ttatcaaccg tttggcggag
    36241 gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga cctctctcaa aaatggcttg
    36301 attatgtaac gaatcaggaa aatcaagaaa aattctttga aatggtaaat gaaatccctg
    36361 caaaccaaca agctagggaa acagcaaagg ctaaaaacga tgaattaaca acagctgtga
    36421 ttgaacaata tgaaacagct caagcgatgc caaacatccc agaaatgggc gaagtttgga
    36481 caggtgcaga aaacttgatg ttcgatgcag catcaggttc aaaaacacca aaagaatccg
    36541 cagatgaagc agtaaaaaca atcagtgaag ccatagaaca aaaatactga ttaaaatgat
    36601 aaccgtccat ttttataaaa aatggaacag gtgcttcaga taaaagtggc aagagcgaga
    36661 tggaaaatta tcttgctctt gttgccatta tcttaaaagc agtttgcggt ttagcaagtt
    36721 tagagggggc attcagatgc agcaagataa aacaagcaat atcaacgaaa aaaaaatcac
    36781 gctttgttca cttattccag ggatggggca gtttgtaaat ggccaagctt ttaaaggtat
    36841 cgtttttttc gccatattcg tcctattcat tggtcagatg cttctgggcg gatttcatgc
    36901 attggtagga ttggttactt tgggaagtat accaatggaa gatcattcat tatttatttt
    36961 gattgaaggc actttgcaat tcatcattac aatcattttc ttgatttttt acggtttgaa
    37021 tcttttagac gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac
    37081 gatgaaggaa gtcatacata atataggtga tgaaggtttt ccttatctat tgacacttcc
    37141 cgcttatctt ctcatgattt ttacgatcat tttcccagtc ctagttacat tgttcactgc
    37201 atttactaat tatgacttca atcatattcc accagccagt ttaattgatt gggtaggggc
    37261 taaaaacttt tttagtatct tctttttaag ctcatatcgt aatacttttt ttgcagtctt
    37321 ttcgtggacc gtgatttgga ctttatgtgc aacaactttg caaatcaccc ttggtattct
    37381 cacagcggtt gttgctcacc agaaatttat taaattcaaa cgtattttcg gtatcatttt
    37441 cttattacca tgggcggtcc cagcttttat tactatcatg agtttttcga atatattcaa
    37501 cgatagcgta ggtgccatca atactcaagt gattcctttc ataaaccagc ttccttttgt
    37561 agatatcagt gcaatctctt ggaaaacgga cccgaactgg acgaaagtag caattatctt
    37621 agtgcagggc tggttaggat ttccctatat ctatgtgatg gttacaggaa tcttacaatc
    37681 gatttcagaa gacttatatg aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt
    37741 ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg ccaacattta ttacacagta
    37801 tacggggaat ttcaataatt tctccatgat ctatctgttt aacaatggcg gtcccggaag
    37861 cgtaggcgga ggagctggtt ctactgatat tcttatttcc tggatctata aacttacgac
    37921 tggaacttct ccgcagtatt ctatggcttc agcaatcacg ttgattatct ccgtcatcgt
    37981 tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca
    38041 atgaaaaaat ttcattcgag tgctcgattc caaaaacgac tttctcactt ttttacttac
    38101 ttttttcttt taatactgtc agtcattatc atctatccgc ttttgatcac agcaagtact
    38161 gccttcaagt caggaaatat tgcagctttt agtttagatc tttcaagtga ctggacttta
    38221 ggaaacttta ctcggctatt cagtgaaaca ctttacggga cttggtataa aaatacattg
    38281 atcatttctg tcctaacaat gatcgtacaa gtgactgtag taactttggc gggctatact
    38341 tatagtcgct atcgttttat cggccgcaaa tcaagtttga tgttcttttt agtgattcag
    38401 atggtaccga ctatggcagc attgactgcc ttttatgtca tggctttctt attagaagcg
    38461 ctcgataagt actggttctt gacaatgatc tatataggcg gtggaattcc aatgaatact
    38521 tggctgatga aaggatactt tgacactgta ccaatagatt tagatgaatc tgctaaactt
    38581 gatggggcag ggcatttcag gatttttttc cagattgtat taccgcttgt caaaccgatg
    38641 ctcgccgttc aagcattatg ggcttttatg ggaccttttg gggatttcat gttagctaag
    38701 ttcctgcttc gaacacctga aaatcagact gttgctgtcg gcttacaaac atttatttct
    38761 gatgtgacaa accaaagagt ctctcttttt gctgctgggg caattttgat tgcactaccg
    38821 atttgtctat tattcttctt tttacaaaag aactttgtta ccggcctgac atctggcgga
    38881 acaaaaggat aggagcagag tatcatgcaa acatttccgt taaactattt ttccgcatta
    38941 cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat catggcctaa atttttcttg
    39001 atatttgtat ttttagtttc cttgatggtc atgcctgtga ctctttttta tgccaatcag
    39061 atccaagcga ttccattaga acaatttcta tccgttcatt cgctgatcga tgaacaagga
    39121 acccaaaaat tttctgaact tgaattatct gaaactgggt tacaagcatc gcaacaaacg
    39181 atagctgtga cccctgaaat tttggtaggt gtgtctttat ctgagaaaca acaatccgat
    39241 catggaactt ttattgattt tgagaaggaa caatgggtga tccaacaaaa ggataaatct
    39301 ggtatacgcc gctatacgat gaattatagc ccatctttcc aaccagattc tgtacgtacg
    39361 cccgaagatt tccaacgatt tttagaaaga gaattttatg ccagtaatcg tccaacaatc
    39421 attttatctt atagtttaag tcttggtctt gtcctatttg tcatgacgag tctcatttta
    39481 ttcggggctt cctttttctt gtggatgaca agaaaaagcc aactttcatc cattcataca
    39541 ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa taggttccat ggcagcagca
    39601 gttgtagggt ttatccactt tgactttatc ttgatgctaa gtgtccaaac aacgataact
    39661 gtcttgctgc ttttatgggt ttttgtcaaa acaagattca aagataagcg tgtcgaatga
    39721 caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt
    39781 gaaaaatttc tgaaaaaatt aaacttctta gttgattctt ttctttttta gtattaacgt
    39841 aatagggtag tttttttaag gaggaaaatc atgttagaga aaactttata caatcaatta
    39901 ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa
    39961 tacggagata cggacactcc tacccaaatc aaaatcaata tccatgaaga aatcccaatg
    40021 aaagagatta caagtaacgc atctttggca ctaggagaag cctatatgga tcatcgaatc
    40081 gagatcgagg gaagtatcca agcattgatc aacgatgctt atcagcaagc aaacagcttt
    40141 ttacgaggaa aagattatct aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa
    40201 aacaaagaag atatccatgc gcattatgac ttaggtaacg acttttataa actttggcta
    40261 gatccaactt tgacttattc ttgtgcttat ttcaatacac ctgaagatac gttggaacaa
    40321 gcacaaatca ataaagtcca tcatatctta gacaaactct ttatcaaaga aggcgaaacg
    40381 ttgcttgata tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa agagtataat
    40441 gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg atttgattac caaaagaata
    40501 aaagaagaac atttagaaga taaatgccgt gtattattaa tggattaccg cgaactaaaa
    40561 ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat
    40621 cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac caaaaggaac agctttgatc
    40681 catggaatca gccgacaaca aggcggtgcg acaaatgctt ggatcaataa atatattttc
    40741 cctggcggtt atgtccctgg tgttactgaa ttagttgata atattacaaa aaacgatttg
    40801 caactgattg atttggaaag cttacgtcgt gactaccaat tgacattaga acattggaca
    40861 aagaattttc atcaagtcca agatcaagtc acgaaagaaa tgggtgattc cttctatcga
    40921 atgtgggatc tatatttaca agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg
    40981 attcaatatt tgcttgttca acctggaaac aatgacatcc caatgcatcg tgcataattg
    41041 aggcaaaagt actgtttaaa gactatttaa aaagaagcta agttttatga tgctccagaa
    41101 gtgaactttt ttgtctaact tctaggattg tctctaaagc ttagtttctt tttctttata
    41161 ttttcataat aaaagtttgg ttctttaatt tttaaacatg acagaaaatt ttttcttcgt
    41221 tatacttgta atagaatact attttataac aaaggggatc gaatcaatga aaaaaactgt
    41281 tatttatact agtttatttg cagctttgtt tttagctgct tgtcaatcaa ataccaatca
    41341 aggcacatct gggaatacgt cttccagtag cgaacagcgc tcttcacagg tatcggactc
    41401 ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa agttctagtg aagcaagtac
    41461 tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc
    41521 agatactgca attactagtt tggaattaga tagtgattgg ggatcttact tctacaagat
    41581 agaaggtgtc gatgatcaaa aagaatacga agcaagaatt gatgctcaaa atgaaaaaat
    41641 ggagacagag aatccagaaa ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga
    41701 agatggtctg gatgttgata aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga
    41761 caaagtagga agcggtacgg caacagattg ggatttggat aaagatttag gtactactta
    41821 ttgggaagtc aaagtaaaag acggcaatca aagtacatca gtcaagatca atagccaaac
    41881 aggcgaagta ctgtcaactg aacaagatga ttaagaattt cttcggaaaa caagagttga
    41941 tttttctttt agtcatgata cactagacaa aataacttgt ttgaaggagc gatttacttg
    42001 agtttagaaa cgacctttgc caatcatatc tttgccaatc ctttgatgaa tgcatctggt
    42061 gtccactgca tgactaccca ggaattagat gaactggctc attcagaagc aggagcattt
    42121 atcactaaga gctgtacgat caatgaacga aaaggaaatc ccgaaccacg ctattttgat
    42181 gttcctttag ggagtatcaa ttctatggga cttccaaact taggtttttc ttattatcta
    42241 gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc
    42301 attgccggga tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg
    42361 tttaatggga tcactgaatt aaatttatct tgtcccaatg tgcctggaaa accacaacta
    42421 gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag tattcagcat cttttctaag
    42481 ccgctaggaa tcaaattgcc tccttatttt gattttgctc attttgatca gatggctgat
    42541 atcttgaatc agtttccatt gacctatgtc aatgctatca acagtgtggg caatggtttg
    42601 tatatcgata cagaacaaga agcagtagtc atcaaaccaa aagaagggtt cggcggcatt
    42661 ggcggagagt atatcaagcc gactgctttg gcgaatgttc gcgcttttta tacacgtttg
    42721 aaaccagaaa tccaaatcat tggtacaggt gggattcgga caggacaaga tgcatttgaa
    42781 catctgctat gtggcgcaag catgttacag attggtaccg agcttcataa agaagggccg
    42841 gaaatttttt ctagaatcat aaaagaatta acgcagatta tgtcagaaaa aggatatacg
    42901 tcaattgacg aatttaaagg caaattacgc accatttcat agcaaagaga cgaaggtcat
    42961 gattgatctt cgtctttttt tataaaaaac actcgtctgt tcattcctgt gcaagcgtac
    43021 tcattgtatg ttagacgaac ttgtgggaaa ataacagagg caaaaaggag gataatatga
    43081 atgatgcaac agaattagca aaagagctta aagaaaaaga atttagtttt caagaactag
    43141 ttacggatat acagaaaaaa ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc
    43201 ttgaaccatt cgagagtaat tcccacgagc tattgattcc ccagtcagaa aaagggccat
    43261 tggctggtat cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta
    43321 cttcaggatc tcgtttattt gaaacacacc gagcttctca tacatctaat tatgtgcggc
    43381 aagcagaagc gataggtcta gttccttttg gacaaaccaa tgcgccagag tttggtttca
    43441 aaaatattac agacccagtc atctatggcc cagcaagaaa cccttggaac cttgaatgta
    43501 caccgggcgg atcaagtgga ggagctgctg ctgcagtttc atcaggaatc gttcctttag
    43561 ctggtgctag tgatggcggt ggttctatta gaatacccgc ttcgttttgc ggattgattg
    43621 ggctgaaacc ttcgcgaggg acgatgccag ttggtccata tgcttggaga ggatggcaag
    43681 gagctgctat tgatttcggc ttgacagttt ctatgagaga cacggaggct ttattttatg
    43741 ggatgagaag tatccattcc ggtgcgccat atcaagtatc gccagttgag tggcaaacac
    43801 atccaagaaa aaaacggttg aagatagctt tttgtgttga ttcaccgatc cactcacaga
    43861 ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga tttccttgga aaagaagggc
    43921 atgatttgac agagattcct tacccactag atggtcgtaa actgatcacg tcttattatc
    43981 agatgaatgg cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc atccagcgag
    44041 ctgtcagaaa agaagaactt gaacccattt cttggacttt gttccaatat ggtcagaagc
    44101 tctctgccgc tacttacatc gattctctgc atgtatggga tgaagcagca attgtcatgg
    44161 aaaacctaca tcaagaatac gatctattcc tttcaccaac gactgcattt accgccccta
    44221 aaatcagcga agatttgcaa agtgatgcga taagagcaca gatggaacac atcagtgatt
    44281 gttctgaagt agaaggttta gacgttgttt cttccatgtt tgaggagagc ttgaagatca
    44341 caccttatac acagttggct aatctgactg gacaaccagc aatcagcttg cctactcatt
    44401 tgacaaaaga aaacatgcct ctaggaatcc aatttatggc tgccagagga agagaagatt
    44461 tgctatttca aatcggctat ctatttgaag caaatcatca gttccattta ccaaatccat
    44521 ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa agattgtttg ctctaataac
    44581 tagtgccttt tttattttaa gcgcttgcgg tacagctgcc gctaatcagg aagataacag
    44641 ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg acttctggca cactgtttcc
    44701 cgcatcttat tataatgatc aaaatgaatt agttggttat gatgtagatt tggcaaaaga
    44761 ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat
    44821 cacttctgtt tcaaaaagag aatcagattt tgctgcgaac gattttggta tcacaaaaga
    44881 aagagaaaaa aaattctccc tttcagagac tttcaaatat tcatttgaca gtatgatcgt
    44941 acgaaaaaaa gatgattcag gaatccactc acttgaagat cttaaaggga aaaaagcagc
    45001 tggcgaaccc aatactagct atatgcgttt agcggaaaaa cacggagcta aattagtcac
    45061 atatgacaat gccacgaatg atcagtattt aagcgatgta gcaaatggtc gaacagatgt
    45121 tattctaaat gattattatc tgcagaaaat ggccgtatct gctttaccag atatccctgt
    45181 aaaaatactg gagaacgttt atttcaatga aaacgaaagt ggttttttat tgaaagaagg
    45241 aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag aagatgggac
    45301 gacaaagaaa atatctgaaa aatattttca aactgatgtt tcagtcaaac cgaaagaaac
    45361 gatggctgag tcggtgagtg agtgatgtac tttcctctaa ttgatttttc cctcatgtgg
    45421 acttcacttc ctttcgtact gcaaggcttg gtatacacat tagggattgg cttcgtgtcg
    45481 tttcttttag gaaatctggt agggttgctt ttgacagttt taggtctttt ggattggctg
    45541 cctctgaatg tatttattcg tttttattta tcgttcttta gaggcattcc cgcactagtc
    45601 ctgctgtttc tgttatattt tgggttacct tatcaattaa gtgccttaac ggcttcagtc
    45661 atctgcttta cgattaccag cagtgcattt attggtgaaa tctatcgagg atcactagca
    45721 ggcgtttctt caggtcagtg ggacgctggt ttagcttctg gtttttcttt ctttggggta
    45781 ttgaaatata tcgttttgcc gcaagctttt cgaatctcag taccggcact cagtaacgta
    45841 gctatggatc tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc agaaatattt
    45901 caaaaagcaa agatcatagg cggtcggaca ttcgattatt tttctctcta tattttagtt
    45961 gcactgattt attgggtcat ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa
    46021 cgttctatgc atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt
    46081 ctattttttg tttcttgtat attctgccta gtattttaaa accatttcat gtaaaatgta
    46141 aatcgaaaat atgttcggag gtagaaaaca tgaagacccc gctaaccatc gaaatagaat
    46201 cctcgttata ccaatattgc atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc
    46261 ctgatgacca aggaattgta gatacgctta gctgcttgac aaaatttgat ggaagccgag
    46321 aatggcgatg ctatgagctg aaagtgacaa aagcagattt tcgctctaca gccaaactgt
    46381 catttgttgg acattataat tattttgttt taccaagtaa actatatgaa gaagtaaaaa
    46441 ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga
    46501 tgcctgcctc tggtacattt ctgattgcga aaaaagcagc aaagaaagag cttgctgtac
    46561 cggaagaagc tttgacaaac cgcttcatgg cttctttatt cagggaagta agaaaagcaa
    46621 agcaaatgga gcgaggcgta aaatattttc caacagatca attgtacaaa gaattgaaaa
    46681 gacgttcaga aaatcaagat ccattttcta acgaacgata ttatgaacgg tttttagagg
    46741 aaatagctag tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt cagcatgatt
    46801 atgaatattt gaggcaacaa cagcagatcc gtcggttgcc tacagagcca ttggagtgaa
    46861 aaatatgtac tatccttatt taagaggaaa acaatttgac ctattagcat taaaagaagc
    46921 actgtcgcga ggattgctct ccaataaaat ccaaccagtg atcgaaccag tacgtgattc
    46981 cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag caccatcgaa tcgctgtgat
    47041 ccaaaatccc caagtaggac aattcaaatt attcgatcag catgtgcaca gttggacagt
    47101 caaagaaaat agctctgtcg tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt
    47161 actggattca cctccagcgt ttcttgtttt cgatggacag cactacccaa aagatacaga
    47221 ggtgtggaaa cagttggcag gagttgattc gaaatttttg ataccagata ctagtcgctt
    47281 tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa cgagaaagca
    47341 tgttgaaagt tatgcagata aaacagatga cttttttagt gatgactatc tttttttcca
    47401 cgcagacgga tatattggat tttctgattt cacgatcgaa ggtagccgtt atttcgataa
    47461 agggttccct agccgagcgc ttgctatcca tctaacgtat attgatgctt acggcaatat
    47521 acgcgtgaaa cattttgtct ctgacagtaa cgactctgct aaagaccaag cattgaagtt
    47581 tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc aactcttgat
    47641 tacttcagga atgattgaat tattggctct ttaccagcaa caaaaattcc ctggactggg
    47701 agtgttgaaa aagtggtcac ttatgcatca tttggaactt attagtcacc tgctagatca
    47761 tccaaaagat tggcttaaat attattcaaa agttcaggaa ctatatgaag tgatccaatg
    47821 attagataat gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat attcggcaaa
    47881 caaggaaaga agaagtagaa gaagtggtag aaatcattaa tgccgcagcg gatttcattg
    47941 cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa acgattttga
    48001 acgatattga aagaaaagaa agctatgtat taatagtgga agatcaaatt gctggaacag
    48061 ctgcattagt ctcaagcgtg gatcccgttt acacaaaaat agatggtagt tggaaagaat
    48121 caactggtcc ttatttatcg attcatcgtg tcgctttagc agaaagtact cgaggaaaaa
    48181 aactagcaaa accgttctta gaacatttgt tgacgattgc agccagcaaa aatatccaag
    48241 atatccgtat cgatacttat ccagctaatg aaccgatgga aaagaccatc tattctgctg
    48301 gattcactta tcaagggatg attgaatttc cttttgaaca cggcgaaaga aaagcatatc
    48361 aaataatcat caattgacta taaaatcatc tgagcaaaga taaagacatt aacactactg
    48421 aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca
    48481 agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa
    48541 tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac atagcttttg tcacgacctc
    48601 tcggcttatc ctacgagtat tattcgatac atttatatca ataatccttt ttttatttta
    48661 tctgcagctt cttggaacgc tccatcgttt gacacgacta aaagtgaggg gatagctttc
    48721 aaacgtttca actgtgctaa atcttctaca taaaccgcca gtctggctga aatctgttca
    48781 gatgaatctc cacgtttttt caggcgcgat tgcaatgttt ctttagagat cgtaataaag
    48841 ataggacgga ctaccgtccc gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa
    48901 acggcgtaac aatttcctac tgtcagtttc tttttaattt ctgctttact ggaaccatat
    48961 ttatgttgag catattggtc ccactctgca aactcatttc ccttgatcat ctgattgaat
    49021 gtattgtcag aaacaaaata ataatcctct tgatcttttt ccccaggtct tggtgatcga
    49081 ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag cttctgcgag tgaagttttt
    49141 ccagaaccag aaggaccgat aaatacatag caataattca ttgtcatttc tcctttgatg
    49201 attgaacaag tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt aactttgctt
    49261 ttttgtatat tataccagca agccagtcac aatagcactt aaaaagctga ctaaagtcgc
    49321 tgttagcaaa attttcaatc cgtgttttgc gacaagattt cctttttctt catttaatcc
    49381 cttcatcgca ccagaaatga tcccgataga agagaagttg gcaaatgaaa cgaggaagac
    49441 agatataatt gctgttgtac gctcagtaaa atggaaatta ccatttgcta attctgtcat
    49501 agcaacaaat tcatttgtaa ccagtttagt cgccatgata ctaccggcat ccactgcttc
    49561 tttccatggg attccgctga tgaaggcaag aggggcaaag atgtaaccta ggatctcttg
    49621 gaaagtgatg ccgaatatcc cgtggaagat agcattgatc atggcaatca aagcaacaaa
    49681 accgataagc atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc
    49741 caacatttgg aaaaatgtct gttctttgtt ttcttcaatg atcagctcat cttctttttc
    49801 ttctaataca tagggattta caattgaagc cagaatataa ccgccaaata agttcaaaac
    49861 aagggcagtg atcacatatt tagaatcgat cagtgccata taagatccga cgatcgacat
    49921 agaaactgta gacatagccg atacactcat ggtgaacaaa cgtttctcag ataggaaggg
    49981 taattccttt tttatggaaa taaaaacttc cgattgtcct aaaatagcag aagctactcc
    50041 attataagat tccaatttgc ccatcccgtt gattttactt aatcctagac caacagcttt
    50101 cataaataat ggaagaatct tgatatagcg tagaataccg ataatagcag aaataaatac
    50161 aattggcatc aatacggaaa gaaagaaagg tgcacttcct ttatttgcta caccggcaaa
    50221 aacaaagtca acaccttgcc cagcaaaagc aagaagatga tcaaacattt ttgccaatac
    50281 agaaacaacc gctgtaccaa aagttgtacg taataatacg aaaccaagaa caagctgtaa
    50341 aacgaacatc acaattagtg gtttcaattt gattgctttt ttgttactgc tgatcaaaaa
    50401 agcaagccct aatgtgacaa ataaaccaat aatacctaat aaataactca aataatcact
    50461 cctattccaa attccgtttt attttacctg ttttgcaaat aaaatactaa tctttagtga
    50521 aattttcatt acttttttag agtgaaacgc attcattttc cattagaaaa taaagcgaac
    50581 aaaataaaag gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc
    50641 gattaagcta atagtttcgt tttcaacatt tataagtaaa ctttttctat tggtaatata
    50701 gattttcgga agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt
    50761 gttcatcatt tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta
    50821 gtttatcata agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca
    50881 agacttcatt gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt
    50941 gtattgtttc aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat
    51001 gttttactgc atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg
    51061 taatgatgac ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac
    51121 tttgcgcttg aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag
    51181 gacgtaaacc gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca
    51241 atcctttttt gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg
    51301 cttcttggac aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag
    51361 taggagattg ataaacagat tctttaccag taattttttc aataatacgt ttgattacag
    51421 gaaatgtctc aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga
    51481 aggaatcgat catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca
    51541 gcggatgttt caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg
    51601 catgtccttt ttggctttca tgatacagtt ggttcaagga agtagcgagc tttccacttc
    51661 ctgggccagg agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc
    51721 caaaaccatc ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt
    51781 gtttatagac cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct
    51841 gtccactata gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat
    51901 cgattaaccg caagatatcc atatcataag taatcccata atcaccacgg atcttattac
    51961 gttcaatatc tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca
    52021 gcaatttgat tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt
    52081 caactagctt cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa
    52141 tatacgctga ctgctcttca atatattttt ctgaatcaaa accaattttt ttcactaggt
    52201 tccctcctta agtactaaag cattttttca tttttttgag gattcgtcaa ctggaagagc
    52261 gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat
    52321 tggtatgata gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac
    52381 gcatataaac actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc
    52441 gaattggaac aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag
    52501 catcagcgca ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg
    52561 tcatcggatt cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt
    52621 accaaatttt ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt
    52681 ttacagaaaa tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc
    52741 aagtagtatg aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac
    52801 gaatgcggat caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc
    52861 aagcatgaat ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga
    52921 agaagatatc cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag
    52981 aagctcaaat gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt
    53041 tttacaggaa tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta
    53101 caaaatcggc acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga
    53161 aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac
    53221 ggactatgac aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat
    53281 tgaacgttta gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa
    53341 taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa
    53401 gcaagtacta tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat
    53461 ccagcagcaa aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat
    53521 cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca
    53581 agcaatccat gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa
    53641 tgaaggaggt tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac
    53701 aaacagaaga tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg
    53761 atgacacaga agaaatccct atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa
    53821 ctccgacgaa tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg
    53881 ggtatttaga aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg
    53941 aagcagaatt agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat
    54001 tattagaatc atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga
    54061 caagcctgat cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg
    54121 aacggtcaaa ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt
    54181 tgaatggaca aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc
    54241 aagtggaatc gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag
    54301 atgaccgggt gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc
    54361 agatcacgat attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt
    54421 ttaccgttaa tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg
    54481 aatttgaatt gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat
    54541 cctacgatat ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt
    54601 ctttaacgat aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact
    54661 acccacgatc tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga
    54721 gaaagtcctg attggataag ttggattacc agatgtcatt cagataattc tgttccaaag
    54781 gacttgcggc agttatccag aataattgtc acaagtcttt tctgtatgta ctaaatggac
    54841 gtgaataact tgctgatgaa ggagggattt cattaggaat gtccacaaat aaaatagcac
    54901 agcttagctt attgtctgct gcttgtgtag caggtaggat cgcttttcaa tttattccga
    54961 atttccagcc aatgactgct atttttttat ttatagtact ttacttaagc ttaaaagatg
    55021 cgttaattgt catgagtctt tctattgcga tcagcagttt ttattttggt gttggaccat
    55081 gggtcatcgg ccaatggatc agctatactg ttgttctgag tttgttttca atcgtctgct
    55141 tgagaaaaac agtgcaaagg aatctatggt taaagggagt ctgttttttt ctggcgggca
    55201 tggtttacgg aataggtatg accgtttttg atacgctgtt atatcgtttg cctcaacctt
    55261 ggatctatta ccttcaaggg gtgtcgtttg atttgatgca cagtataggc aatgttgtgt
    55321 tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg
    55381 aaaaaaataa tttatagttt gcttgcaatg agtgtcagta tactcatttt gacaagttgc
    55441 agtacaacct atacacaatc ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa
    55501 gcaacaatca cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa
    55561 aaaggcgatg atttactgag tattttgaaa cgaaattttg aagtcaaaga agataacggc
    55621 ttcatcactt cgcttgaagg gcactcacag gatgagaaaa atcaactcta ttggatgttt
    55681 actgtggacg ggaaaagtgc tactactggt gccaaagaga tcaaattgaa tgatggacaa
    55741 tcggtcattt tcaatttgtc taaactataa gtttcataaa aaactgttaa agggcgaata
    55801 ttgccataat tcagtgagtt cagttgatga aagaaggaaa ataacatgga aaattttaaa
    55861 aaaagcggaa tgttttccgt aattgctgct ctaggggcta atattctagt tgctatcagc
    55921 aaatttgtcg gctatgcaat cagtggcagt gccgccatgc taaacgaaag catccacagt
    55981 atcgttgatt gcagcaatca gattttgctg ttattcggtg ataaacaagc caataaagga
    56041 cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat actttttcag tacaatcgtc
    56101 gcaatgatgc ttttcttcgg cggtggggca cttggggtga tggaagcttt tgaaaagttg
    56161 acgcatccat cccatgaagt gggtaatccg atcattgtta tagcaatact gttatttggt
    56221 ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga attgaatact
    56281 gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat actgattatt
    56341 tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg aacaatattg
    56401 acttatgcaa ccgggattgc tgcatttgat gcgatcagcg gattgctgat cggtttcttg
    56461 ctgatgggag ccgccatttt cctagcaaaa gaattttata gtctgatcat cggtgaaagc
    56521 gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg
    56581 ctgatcgata taaaaacagt tcatttaagt ccaacggaga ttttagttgc agcaaaagca
    56641 gatatcgttg gtaacaaaga gaatgaagct tactcggtca tcaatgatat tgaaaaaaat
    56701 atgcgtcagt ctcttcctga caagaagatg tatatataca ttgaaacaga taaatatgac
    56761 ccaaattatt ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat gagaaaaata
    56821 taatcttgtt tttaaataaa acggctacaa tttagcgaat ttatgttaca atgagtccgc
    56881 tgtttatttg ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg
    56941 cttcaagctt tgcatgcagt gatgccactt tgtataagct atataccagt aggattagct
    57001 tgcggagtat tgttgcaaaa agttggattt gatcctctct tatcaggact gttatcgatt
    57061 ctggtctttt ccggtggcgc gcagttttta gttgcatcta tgcttacgac gcaagcatca
    57121 tttgcaacga cattattgat ggttttcttt ttagaattac gctatacatt attaggagca
    57181 agtcttgccg gttttatgaa aaaagaaaag cggacgtttt tggctgtctt ttctcaatca
    57241 ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt caactgaccc ttcctggaat
    57301 aaaagaaagg ctttatatgt caattggttc tcaatgactg catgggccgg tagcaatatg
    57361 atcggtaatt ttttcggctc ggtgattcga gtagatgctg accttgtcca ttttgctttg
    57421 acggccatgt tcattttcat gtttatcatg cagatgaaaa gcgcactact gatctttaca
    57481 ggtttattct caggcgtatt aggggtcgta ttcatggtat tattccaaaa tactttcgga
    57541 ttgattgcag caacagtcat tgcttcattt gccggattta tgctggagaa ggcattcaaa
    57601 aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac
    57661 ttcccggaac aggaggccaa tcaccatgac taaactatat ttgctacttt tagttgcttg
    57721 tctgttcatc gtggcatata tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa
    57781 agtgccatcc tggttcagtg actggatgaa atatgtaccg gtggcattat ttgcggcgtt
    57841 agcttttaaa gatgttttca ttactcatga acatctggat attgcttgga atatcaaaat
    57901 agctgcaatg attctagtag caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt
    57961 tttgacagga cttgcgtctg tctttttgct gtctatgctt taaactgttt tattttatta
    58021 aaaacagaga ttgcgacata agttgttcaa actgatctat ccgaactgca tttgcaacag
    58081 tcaattctgg atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc
    58141 aagctctttt ttattttagg aattagagag ggatttgtcc ttctatgatt ttttgctata
    58201 atagggaaga tgtttatttt atgaaagaat agttgaggga atttaaatga ataaaaaatt
    58261 gatgcaagga acattttggc taacctttgc taatcttcta tgtaaagttt taggggtagt
    58321 ctatttgatt ccctggctaa gtatgatggg gaacaatcaa gatggtatgc ttgctacgac
    58381 attgtataat gttggctatc ttccttatgg attgttcctg atgcttggta cagtcggatt
    58441 tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa gaaggagata acgctgcttg
    58501 tcggctgatt tttagaagta cgatcaatat catgtttgtc atcggtatcg cctctgctgc
    58561 actgatgtat ttatttgctc ctttactagc cggaatcagt ccgattgcta acgttaataa
    58621 tgggatacta gctatcagaa gtttatgccc atctttgatt gctatcccaa ttcttagtgc
    58681 aatgagagga tatttccaag gaaaaaatga tctgcggcct tatggaactt ctttgattat
    58741 cgaacaggct gtgcgagtga tcgttatctt ggctggtact tattacttgc gcgtattgac
    58801 tgacggaacc atattagaag ctgtattgat cagtacagtc gcttctttct tcggtgggtt
    58861 agctgcgatt atccatatgt atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga
    58921 ttttttgatt tccagccgtt atttcgaaag agaaaaccga tctgcctctg tctctatcat
    58981 tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt actatcgttc aaatgatcga
    59041 ccaagtaacg atgaaaccac tgcttcactt tttccgtcca gagatcgcag atcagcaact
    59101 ggaattctta ttcagccgtg cttctgtcaa tccaaataaa ttgacattga tcctgatctc
    59161 catggtagga acagtagcta taagcagctt gccaatatta agcacgatga agaaaaaaga
    59221 tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg attgcgttat tgattcttct
    59281 tccttcattg actggaatgt ctttattagc tggaccacta tacacattgt tttttggcta
    59341 tgatcctgaa agtgtcggat atttccaaat ggctttgcta gcttctttgt ttttctcttt
    59401 attcacgatc ctatctacga tgcttcaatc tttgaatcac catcgttttg cgatccgatt
    59461 aactgtagaa gcaattatcc tgaaagtcat cttccaagta atcggattag gattagttgg
    59521 cggatacggg atgagcttat caaatggctt agcattcggt attgtctttg ttcgcggcta
    59581 tcactttatg tgcaaagaat accgaatagc acctttagca aagatcagca attttttctt
    59641 aaaaactttc cgctccaccc tgattatgct tgcagtatgt tttgccgttt tctttttgct
    59701 gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg tggctgtagg
    59761 aagtattggc ggcttagtat ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt
    59821 gaaaaatgtt aaacatcata gcagaagcaa agaataggtt aactaatcgt tttttgctgt
    59881 cacttgaaaa acggcaactc gtatgattac gcttgtcgtt tttctatttt ccaattttag
    59941 tttcatatct aatcttactt atgatatagt tataaggtct ggaaaaattc tttgaaacga
    60001 ggaaattaaa cacatgaaac ttgcagtagt tacagatagt tcggcatatc tgccggaacg
    60061 tatcaagaac catcccgact tattcgttat tccaatccct ttgattatgg atgggaaaat
    60121 ttataacgaa ggaatcgata tcgaagcgga tgaatattat ggacttttaa aaaacagtaa
    60181 agattttcct actacttcac aacctgtcgt aggggaagtt ttgggtttat atgaggaatt
    60241 gaaagagaaa gggtatgaca cgattttaag tatccatctt tcttcaggga tttctggctt
    60301 tgtcaacaca ttatttgcga tgaaagatga catcagcgga gtaacggtca ttccttatga
    60361 ctctaagatc actagtatgc cgatgggaca catggtagaa gctgccttag acttaaatga
    60421 tgaaggtcag tcccttgatg aaatacttgc acatatcgat cgaatccgag ataataccta
    60481 cgcttatctg attgtagatg atttgaacaa tttagtacga ggaggacgtt tgacaaatgg
    60541 tgcagcgctg attggcagct tattaaaaat caaaccgatc ctgacatttt ctgaagggaa
    60601 aattgtttta ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag ctgaagaaat
    60661 tattggtaga agaaataacg aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa
    60721 taatttagaa gtagcgattg cggagaaaga gaaattgcag aaaaaatatc caaatgcaga
    60781 aatcgaaatt ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa aagccatcgg
    60841 cattgccatt tcagcgcaat aattcctttt attcaaaaaa cataccaaaa atattccaac
    60901 atcatcttgg actttctaca tgttactaat gagcgtgtaa aagaacgaga tggtgttttt
    60961 atttctatca atgaagctct ttttctttgc aatcctcact tcaatcctct ataatgtcgc
    61021 atatagttta gtaaatgagt aaactactaa tttacttatt tactaaaaat aaaaggatga
    61081 ggagttaaaa taatggaaat accaacaaat ttaaaacata aaccagtaat cgttgtagaa
    61141 gactatgata aagttgatgg aagaaatgca ttaaatacgg atgcaaaagg gctttcactg
    61201 ggccttgctc agtggaatga tcgtggaaaa gtcgatatct caggcaaggt atggcgacat
    61261 actggagaaa aatggtcacg acagtcggaa gaattaccaa tcacacgtat tttagattta
    61321 gcgatcttag ctgcacaagg aagtttatac ttccaagatg cttatcgtta tgaaaaattt
    61381 tatgatccag aaaatccagt cgtagacatc attggattac aagggaaccg aatgacggta
    61441 gaagtagata ccaaaaatcc aaagatcgat gaagacatca ttttgtatta cgatacacta
    61501 caaaaagacg gagaactgat tggggaacga ttccgtattt taaaacgtct tttagatgag
    61561 ctaggatatt aatagacaga gatgatcaaa aggggaagag aagcatggag aatttctcac
    61621 taacagaaaa ccgaaccttt tcacggctag aaaagcaaat gatctggaag aaaccgaaaa
    61681 gccatcggac aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg aattggcaag
    61741 atcctgaaat gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg gggaagacac
    61801 aattagccaa agcactatct gctgatctgc aacttcccta tacaaaagtg acctgttttg
    61861 cagatatgga caaatcagat gttttcggag ccttattacc tgtagtagat tctaataagg
    61921 aacaagacca agaattacta gaagctattt accaaacaga tacacttgaa gctgtactga
    61981 cactgatcca gaaccattac ggcattgatc ctttttctgc taaagaaaaa ttagcagagc
    62041 tagttcaaag gatcgaagct gattatccag atgcaatcca ttacagctat tacccttctg
    62101 aaatcgttcg agcgattgaa aaaggatatt tattagagat tcaagagccg actgtcatcc
    62161 gagatgcctc tgttcttgtg gcacttaact cagcgctcga accaaatggg atgttgaatt
    62221 taccgacagg aatcattcgt cgccatccag attgcgtcat tgtgatcact acaaatcgaa
    62281 attaccaagg aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa
    62341 aaatggatct tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc
    62401 aaaaacaaga acttctctta aagatggcag agattatccg tttgttggat gaaactgcaa
    62461 aagctaacgc catcaaaggt gtagcaggaa tgcgttctta tttttattgg gcaaatacct
    62521 ataagcaagg acaagattta ctccagtcga tttatccaaa agttttgtat aaattatcca
    62581 ctgattcaga tgaattggca attcttgaac aagctttgtc tgacagtgga ttgctactgg
    62641 aacttcagga catattgaga aaagaaaaat ggggtcatcc tcaatctgag catacaaaag
    62701 gaagaaccat tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa
    62761 ttctgcgctt agatgaaaag aaatcgttgt ctgctgaaga accaaatgag ggagaagaga
    62821 aacaagaaga aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta
    62881 gcgatatcga aggaaaaaat caaaaagaac aacaaacagg agatgattct caagaaatgt
    62941 ctgcttctga catggaagca catgataagg aaatcaagaa gcaactgaat aaagaagcgc
    63001 gacaaatcat gaaggaaacg attcatgaaa aagaaggtct catcgttcac cgaccagctt
    63061 tccaaggaaa aacgccggaa gcacgtgagc tacacatgca agtaatgcca atagtagaat
    63121 cacttagccg gcaaatttta gagttactgg ataatgaaca aacagagacg taccaaaaag
    63181 gaaaatacga aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt
    63241 ttgacaaaaa gaacccgcca catgagcaac cttctttagc ggtagctgta cggatcgatg
    63301 aatctgggtc aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca
    63361 ttgccgaatt ttctaagaaa gtcgatatcc cattgttgat ttacggagat acagcagacc
    63421 gttcgtcaag agaaaagact tctttgtttt cttataaaga atttgaagac ggatttcaat
    63481 ggttaaatga aaaattagtc actatgaaac caaggcaaaa caatcgtgat ggtgctgcac
    63541 ttcgtttagc agcagaaaaa ttaaacagac aatccgcaac aaccaaactg ttgcttaata
    63601 ttagtgacgg tcagccaaaa gcgttgccgg attatacagg caaaaaagca aaagaagata
    63661 tccagtctgt tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc gcagctattg
    63721 gccaagacaa agaagaaatc aagtcgatct acggtgaaag tcgttttgta gatattactg
    63781 acttgaatga atttcccaag caaatgattc aactgattgc ccgctatcta tgataaaata
    63841 aagctcaacg aacgcactta gcagattcgt tgagcttttc gtttctagtt accggtaaca
    63901 aggatacttc taaaatagaa tcggttataa tcaagaggga ccgttaaagg agtgaaacga
    63961 tatgatacaa gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct atcctgctag
    64021 tttcaaagat agcaatggcg atggtcaagg agacatcaat gggatccgtg aaaaacttaa
    64081 ttatttgaaa gatcttggta tcggctttat ctggatcaat ccgatctatc aatctccatt
    64141 tgtcgataat ggttatgacg taagtgacta tcaagatata gaagagagat tcggaacgat
    64201 ggaagatttt gatttattgc taaaagaagc acatgaatta gggatcaaag tgattatgga
    64261 tcttgtcatc aaccatagtt cggatcagca tcaatggttt gaagaatcga aaaaatctaa
    64321 agaaagcccg tatcgcgatt attacatttg ggtcgacggg gtagatggca aagaaccgaa
    64381 taattggaca tcgattttcg gcggttctgc ttgggagtat tctcatgaaa ctggacagta
    64441 ttatctgcat gtttttgcaa aagaacaacc agacttaaat tgggaaagcg agaaattgaa
    64501 agaagaacta ttcaatatga tccgatggtg gttggataaa gggatagatg gatttcgttt
    64561 agatgcgatt tctcatgtaa aaaaagatga atattcagta aaagccacag agaacccttt
    64621 ctcacccttt caaaatgtct caggaatcga agaacatttg accgatttga aacatgtttt
    64681 tgaagagtac gatatcatga ctgtcggaga agcaagtggt gtcactgcag aagaaggacc
    64741 acaatgggta gggaaagacg gttatttcga tatgatattc gaatttgatc atatccatat
    64801 ctggcagcaa gaaaaagaag ggcaattaga tgtccttaaa ttgaaacttg cattaagcgc
    64861 ttggcaaact tcattggatg gtatcggatg gaacgcttta tatatggaaa accatgatgt
    64921 tcctcgtgcc gtttcagtgt ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa
    64981 agcaatcgct atgatgtatt tctttttgca aggtacgcca tttatttatc aaggacagga
    65041 aatcggaatg acgaatatgc cctttgaatc gatcgatcaa gttgatgcag tagattcgaa
    65101 aagactttat aaaaggctgc ttgcggaagg taagacaaga gaagaagcat tggatatcat
    65161 tcgtgaaaca acgagagaca acagcaggac acccatgcaa tggacttcgg aacaatatgc
    65221 tggtttttct acacatgaac cttggctgat cactaatcca aataccaaaa cgatcaatgt
    65281 agaacaacaa gaatatgaac cagagtcggt cctgcaattc tataagaaca tgatccgcat
    65341 tagacaaacc aataaaggtc tgatatacgg ttcttataaa gaatatcttc atgaacatcc
    65401 acaactttat gtctatgaaa gatatttaga agatgaacaa tatcttatta tggtaaactt
    65461 aacagaatca cttgctgact atgaacttcc aaaagaagca gatcagtcct ggacattact
    65521 cttgtcaaat tcctctagcg gagaatttga agctaaaggc atactagcac cttatgaagc
    65581 tcggttatat aaaacaaaca agtaaaactt cctcgttaaa gttcattagt tggactttgc
    65641 gaggaagttt ttcagattgc tctgtcaatt actcatttca ataattcgct ctaggaccac
    65701 cgatatattt aggacgagag cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac
    65761 gaggtctgac aggtacttgg acatgtttga tatgcatacc gattgatgtg tcaccgatat
    65821 cgataccggc ttgtccaact acgtgttcca cttcgacagg atcattgaac tggtcaaaag
    65881 cagcaacaga acaagcacct ccagcatgta gtgcaggaac aacagagaca atttcgaaat
    65941 tttttgcttc tgccactgtt cgttctacta ctaatgctcg gttcaaatgt tcacaacctt
    66001 ggacagctag cgcaatttct ttaggatcca acaactcttt taacgtacgg atgatccatt
    66061 gaccaacttc cgcactggaa ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc
    66121 atcctaaaac aaaaagatca ccttttttca agtgtgcctc agccaatatg tcattaacta
    66181 tttcagtcaa ttggtctttt aattcttttt ctgacaactt cattttctaa aaccttcttt
    66241 ttcaattaat attgcttccg tgcttgaata cgttgaagac ttgcagttat aggaaaggca
    66301 agtcccattc caatcagatt ttggaagatg tttcctggta tagaagccac tccagccggc
    66361 caatcatata aaatccaacc ggcgatacca tagccgatga tcataactaa gcttcctaat
    66421 aaaagcccta atccccacat acgagtagaa gatttgctga aagaaccagc aacatatcct
    66481 tgtaacccgt ggatcactaa tgaaaaaact gcccactgcg gatatcctga aatcaaatcg
    66541 atcaatagac cactggaagc accaacaact aatccacccg ttggaccata aagaaaagca
    66601 gcggtataga tcccggcctc gcataatgtc acaaatccat tcgtagccgg tacagggatc
    66661 aaaattgtta atgaaagggc aactgtcaac gcaatcagca tagcatagat cgttaattgc
    66721 ttagttttca tttctcattc ctcctttttg aataccgcca gaccagacac ttccggtatg
    66781 atcgttcatc agcacgccat tagaaataca atgggcaaca aattttttac tttctgctac
    66841 tgctttggta aaatctgccc ctaacacaag attcgctgtt attgcagaag aaaaggcaca
    66901 accggccccg tttactgttg tagtgtcgat ttttttatga gaaaatacct cataatgatc
    66961 aattgaatag agaagatcca cagcttcatc tccagccatc cttgtgccgc ctttgatcac
    67021 aatgtttttt gttcctgaag cagtgagttc tttagcgaga tggatcattt ctgttaacga
    67081 agagggaaga gcttgaccag aaagtaaagc agcttctgcc aaattaggcg taatcatcgt
    67141 aacaagtgga aaaagttctt cgatgagctg cgaaatatac aattgattga tcacagcttc
    67201 agtttctttg aatgccaaaa cagggtctaa aatgattggg atattttgtt ttgtttttat
    67261 gaagtctcga acaaccatga ttgcttcact tgaattcaat aacccaattt tgataccatc
    67321 taatgaagtc attttatcaa tcgaaactaa ctgtcgttta agcaattcca aagatataga
    67381 ttccagcata aatcgctcat taatatctag tgcccctata gaagtcaagg cgcttagtcc
    67441 gaatagacca aaattctcaa aagtttttaa atctgtttgg attcctccac cagcaaatgg
    67501 atcagaaccg ccaattgtca agattctttt cataacattt tcctccatga ataagagtat
    67561 aacgaaattt tcaaaaaaga aaaacagcca attggcatat ttttatctca tccaatttaa
    67621 aaaaacaaaa agaaaagaaa gaccaaaatg gatctttctt tccttaaata gttaggactt
    67681 ttgtgatttt aattctttct cgatttgaag ccgttgcact ttcattgtag ctgttcgcgg
    67741 gattgcatca aaatcacgga tgatagggac gttcaagcga ggaagttcat ggacttgctc
    67801 ccaccatcga ttccaatcca tttctttatc aggagctaaa gcaataatcg gttgtggaga
    67861 atgattttta tcccttacaa tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa
    67921 gtcttcaata gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa
    67981 aaataggtgg ccatgcttat ccataaaacc gtaatctccg ctatcccacc aatcgccata
    68041 aacattttcc tggaaccgtg catcttcctt gtaataagtc aaagcccgtc ctttagacag
    68101 taattgtata tgaccatctg tgttttcagg taggacattc ccaactgaat ctgtaatccg
    68161 agcgcttgtc atgtcttcca gtccaacgcc catatctctt gcatcacttg ttttcaaact
    68221 ctctagtgta tgagccttaa gaatcattgg tccgcattca ctttggccat atacttgaag
    68281 aaataccgca tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat
    68341 agcatcaaaa gtggagtgat agtatcggat acccgaaaaa gcttctggat gctccttagc
    68401 agtaaaacgc cactgaacaa agttattcgg atgggtctct agtgcaattg gccggtgagc
    68461 ttccaacata tgaacaactt ttgaactttg tgcgtttgct aaaggcatca taggaaatcc
    68521 catggacatc aaagaagaga taccaatatt gaatctggaa tgcactggag agatgtgaaa
    68581 agcgattaat ttcttttccg aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca
    68641 tcccatagaa tgagcagaat gacaaataag tttagggata ccagttgtac cagaagtatg
    68701 ggtcatataa gaaatttcat ttttgtctaa aaatgattga gaaacagaca aagcaggttg
    68761 ttccattaaa taaagaatag g
//
LOCUS       DACEKG010000002        64815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEKG010000002 DACEKG010000000
VERSION     DACEKG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.002"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(80..520)
                     /locus_tag="IW664_000067"
     CDS             complement(80..520)
                     /locus_tag="IW664_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0847465.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(649..1575)
                     /gene="dnaI"
                     /locus_tag="IW664_000068"
     CDS             complement(649..1575)
                     /gene="dnaI"
                     /locus_tag="IW664_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0847466.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(1575..2948)
                     /locus_tag="IW664_000069"
     CDS             complement(1575..2948)
                     /locus_tag="IW664_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0847467.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(2971..3462)
                     /gene="nrdR"
                     /locus_tag="IW664_000070"
     CDS             complement(2971..3462)
                     /gene="nrdR"
                     /locus_tag="IW664_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0847468.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(3774..4106)
                     /locus_tag="IW664_000071"
     CDS             complement(3774..4106)
                     /locus_tag="IW664_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0847469.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(4158..4787)
                     /locus_tag="IW664_000072"
     CDS             complement(4158..4787)
                     /locus_tag="IW664_000072"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0847470.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(4751..5587)
                     /gene="mutM"
                     /locus_tag="IW664_000073"
     CDS             complement(4751..5587)
                     /gene="mutM"
                     /locus_tag="IW664_000073"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0847471.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(5632..8277)
                     /gene="polA"
                     /locus_tag="IW664_000074"
     CDS             complement(5632..8277)
                     /gene="polA"
                     /locus_tag="IW664_000074"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0847472.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(8403..9086)
                     /locus_tag="IW664_000075"
     CDS             complement(8403..9086)
                     /locus_tag="IW664_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0847473.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(9079..9564)
                     /locus_tag="IW664_000076"
     CDS             complement(9079..9564)
                     /locus_tag="IW664_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0847474.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(9871..11205)
                     /locus_tag="IW664_000077"
     CDS             complement(9871..11205)
                     /locus_tag="IW664_000077"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0847475.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(11336..12097)
                     /locus_tag="IW664_000078"
     CDS             complement(11336..12097)
                     /locus_tag="IW664_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0847476.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(12113..12595)
                     /locus_tag="IW664_000079"
     CDS             complement(12113..12595)
                     /locus_tag="IW664_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0847477.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(12615..14795)
                     /locus_tag="IW664_000080"
     CDS             complement(12615..14795)
                     /locus_tag="IW664_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0847478.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(14939..15670)
                     /locus_tag="IW664_000081"
     CDS             complement(14939..15670)
                     /locus_tag="IW664_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0847479.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(15739..16680)
                     /locus_tag="IW664_000082"
     CDS             complement(15739..16680)
                     /locus_tag="IW664_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0847480.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(16860..17228)
                     /locus_tag="IW664_000083"
     CDS             complement(16860..17228)
                     /locus_tag="IW664_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0847481.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(17839..19230)
                     /locus_tag="IW664_000084"
     CDS             complement(17839..19230)
                     /locus_tag="IW664_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0847482.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(19443..19829)
                     /locus_tag="IW664_000085"
     CDS             complement(19443..19829)
                     /locus_tag="IW664_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0847483.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(19885..20508)
                     /locus_tag="IW664_000086"
     CDS             complement(19885..20508)
                     /locus_tag="IW664_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0847484.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(20572..21240)
                     /locus_tag="IW664_000087"
     CDS             complement(20572..21240)
                     /locus_tag="IW664_000087"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0847485.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(21252..21692)
                     /locus_tag="IW664_000088"
     CDS             complement(21252..21692)
                     /locus_tag="IW664_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847486.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(21751..22422)
                     /locus_tag="IW664_000089"
     CDS             complement(21751..22422)
                     /locus_tag="IW664_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0847487.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(22607..23005)
                     /locus_tag="IW664_000090"
     CDS             complement(22607..23005)
                     /locus_tag="IW664_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0847488.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(23002..23775)
                     /locus_tag="IW664_000091"
     CDS             complement(23002..23775)
                     /locus_tag="IW664_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0847489.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(23772..24455)
                     /locus_tag="IW664_000092"
     CDS             complement(23772..24455)
                     /locus_tag="IW664_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0847490.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(24622..25509)
                     /locus_tag="IW664_000093"
     CDS             complement(24622..25509)
                     /locus_tag="IW664_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0847491.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(25506..26375)
                     /locus_tag="IW664_000094"
     CDS             complement(25506..26375)
                     /locus_tag="IW664_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0847492.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(26512..27798)
                     /locus_tag="IW664_000095"
     CDS             complement(26512..27798)
                     /locus_tag="IW664_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0847493.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            28071..28649
                     /locus_tag="IW664_000096"
     CDS             28071..28649
                     /locus_tag="IW664_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0847494.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            28649..29578
                     /locus_tag="IW664_000097"
     CDS             28649..29578
                     /locus_tag="IW664_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0847495.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            29575..31311
                     /locus_tag="IW664_000098"
     CDS             29575..31311
                     /locus_tag="IW664_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0847496.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(31374..32210)
                     /gene="pheA"
                     /locus_tag="IW664_000099"
     CDS             complement(31374..32210)
                     /gene="pheA"
                     /locus_tag="IW664_000099"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0847497.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(32207..32716)
                     /locus_tag="IW664_000100"
     CDS             complement(32207..32716)
                     /locus_tag="IW664_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0847498.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(32709..34004)
                     /gene="aroA"
                     /locus_tag="IW664_000101"
     CDS             complement(32709..34004)
                     /gene="aroA"
                     /locus_tag="IW664_000101"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0847499.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(34017..35108)
                     /locus_tag="IW664_000102"
     CDS             complement(34017..35108)
                     /locus_tag="IW664_000102"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0847500.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(35125..36291)
                     /gene="aroC"
                     /locus_tag="IW664_000103"
     CDS             complement(35125..36291)
                     /gene="aroC"
                     /locus_tag="IW664_000103"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0847501.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(36293..37357)
                     /gene="aroB"
                     /locus_tag="IW664_000104"
     CDS             complement(36293..37357)
                     /gene="aroB"
                     /locus_tag="IW664_000104"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0847502.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(37390..38412)
                     /gene="aroF"
                     /locus_tag="IW664_000105"
     CDS             complement(37390..38412)
                     /gene="aroF"
                     /locus_tag="IW664_000105"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0847503.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(39071..40045)
                     /locus_tag="IW664_000106"
     CDS             complement(39071..40045)
                     /locus_tag="IW664_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0847504.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(40026..41909)
                     /locus_tag="IW664_000107"
     CDS             complement(40026..41909)
                     /locus_tag="IW664_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0847505.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(41927..44152)
                     /locus_tag="IW664_000108"
     CDS             complement(41927..44152)
                     /locus_tag="IW664_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0847506.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            44267..45301
                     /locus_tag="IW664_000109"
     CDS             44267..45301
                     /locus_tag="IW664_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0847507.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(45368..46381)
                     /locus_tag="IW664_000110"
     CDS             complement(45368..46381)
                     /locus_tag="IW664_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0847508.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(46459..46647)
                     /locus_tag="IW664_000111"
     CDS             complement(46459..46647)
                     /locus_tag="IW664_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0847509.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(47087..47215)
                     /locus_tag="IW664_000112"
     CDS             complement(47087..47215)
                     /locus_tag="IW664_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0847510.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(47218..48591)
                     /gene="rlmD"
                     /locus_tag="IW664_000113"
     CDS             complement(47218..48591)
                     /gene="rlmD"
                     /locus_tag="IW664_000113"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0847511.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(48702..49757)
                     /locus_tag="IW664_000114"
     CDS             complement(48702..49757)
                     /locus_tag="IW664_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0847512.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(49767..51197)
                     /gene="gatB"
                     /locus_tag="IW664_000115"
     CDS             complement(49767..51197)
                     /gene="gatB"
                     /locus_tag="IW664_000115"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0847513.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(51194..52666)
                     /gene="gatA"
                     /locus_tag="IW664_000116"
     CDS             complement(51194..52666)
                     /gene="gatA"
                     /locus_tag="IW664_000116"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0847514.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(52666..52971)
                     /gene="gatC"
                     /locus_tag="IW664_000117"
     CDS             complement(52666..52971)
                     /gene="gatC"
                     /locus_tag="IW664_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0847515.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(53077..55113)
                     /gene="ligA"
                     /locus_tag="IW664_000118"
     CDS             complement(53077..55113)
                     /gene="ligA"
                     /locus_tag="IW664_000118"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0847516.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(55130..57364)
                     /gene="pcrA"
                     /locus_tag="IW664_000119"
     CDS             complement(55130..57364)
                     /gene="pcrA"
                     /locus_tag="IW664_000119"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0847517.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSICVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            57519..59078
                     /locus_tag="IW664_000120"
     CDS             57519..59078
                     /locus_tag="IW664_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0847518.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(59189..61120)
                     /locus_tag="IW664_000121"
     CDS             complement(59189..61120)
                     /locus_tag="IW664_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0847519.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(61138..62052)
                     /gene="pfkB"
                     /locus_tag="IW664_000122"
     CDS             complement(61138..62052)
                     /gene="pfkB"
                     /locus_tag="IW664_000122"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0847520.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(62049..62801)
                     /locus_tag="IW664_000123"
     CDS             complement(62049..62801)
                     /locus_tag="IW664_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0847521.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(62961..63170)
                     /locus_tag="IW664_000124"
     CDS             complement(62961..63170)
                     /locus_tag="IW664_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847522.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(63181..64509)
                     /locus_tag="IW664_000125"
     CDS             complement(63181..64509)
                     /locus_tag="IW664_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847523.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        17841 a        14067 c        11488 g        21419 t
ORIGIN      
        1 aattcccata tctccttaaa aaatatacct tttcgttcta taaaacaaca ttttcttatt
       61 tttgaaaaaa tgttattggt tatttgttca atgaaacatc aataatcgaa tctccaggaa
      121 cttgcggtgg gatagacatc gtcatgatcg gcatctcaac taatgtaaat tggactttgt
      181 caccgatttt caattcattt atgtccacgc ttattttttc agcagtagca tttaagatca
      241 ctccgtcatt gctgaatgac gttcccatat tcgctggatc ttctttggcc atgacatcga
      301 cgagtttcaa ctgaagcgtt tcttcttctg cttctacttg agtaatactt tcaatcgttc
      361 cgacaaatat gctttgtctc tccgttttcg tactgcttgt ttttactgtt tgacccgcat
      421 caatcgaatc aacagaacct gtgttgctat ctgcgctacc gcagcctgct aaaagaaaaa
      481 atagaccaac tacagatata ctagaaaaaa tagccctcat aaaaaatcct ccgtttaagt
      541 ttctgcatta cttgttgcgg tgattgtctc cggtcatttc tgtccatata atgattcgct
      601 gacctgcttt tactactagg tcaaatgtca gttggacttg tcaactgttc aaccattgcg
      661 tcggttgcga ccagtcattt cgatttcttt tgtcaaataa cgtatgcgtt ccattatccg
      721 tttcgctttt agcggctctt catctccacg ttgagtgacg gacaaatgct gttccaattc
      781 atccattgta aaattcgatg agaagaaggt tggtaactgt tcctgcatcc gatattgaag
      841 gataacaccg aatacttcgt ctcgtatcca actactcatc gcatctgcac caatgtcatc
      901 gatcatcaaa ataggtgaac gtttcactgc atctaacttt tctgccactt gatcttttcc
      961 gattgcctgt ttcatctcaa cggcaaatgt cggaaaatgg actagcgtcg tggtgaatcc
     1021 agcaatcgct aattctctcg ctgttgctcc taataaatat gtttttccaa taccaaaact
     1081 tcctactaga tacagtcctt tatgataccc ttttggatca gcagtatatt tctctacaaa
     1141 atctagggct tctaagctcg catatcctcg tccatctgta cgttcaaagt tttcaaaaga
     1201 agccgattga atatcttttg gcatatccat tgcttttact cggcttctga tttcttcttg
     1261 tctttgccgt gctaacaatg cttccgttgg tacataagtg acatccacat aatgaaaatt
     1321 caacgtcagc ttcggctcat agccgggagc gatcatcgtt ggatcgtttt tttgatattt
     1381 acgcttttct tgcacgaatt catacaattt agcgtaactt ttttcaatat ccgcttgtgt
     1441 caattcttct tggtgtgcgc ttagaaaagc ttgaacatcc gcatcattta agacttcagc
     1501 gatcatttca gcatactttt cttgataatt gcgtttgctg atgatcctct tcaattcttt
     1561 gcctacgtct tccactagat atcaccctcc ttttttgcca ggtattcttt aaaacggcgg
     1621 tcaatctctg cttttttctc gctgctgatc tgtttttcat cttttggctg gttgacccaa
     1681 tcaggaagct tttcccgtcg aacatttcgc ttgccagttt gataatttgt ttgcttcttt
     1741 tgttttgatt gttttgcttg attacttatt tgccgaatgt gctggatcgc ttcttctgct
     1801 gtggcgattt tcttttgtgc ccattcattt gccactgtat ttacataact ggcatttaag
     1861 ctagcattat tcttgatcac tagtacataa ttcagcaaaa cattgatcac gctactggaa
     1921 agacctgatt gagaaacaag atcttgcagt aaccagtttt cttgtttaga aacatatccg
     1981 cctttttctg acttgattgc ttccagatat ttcatcggtg gaatgctctg tgcttctttt
     2041 atcagctgaa gcgctgcgtt tgtcaaatgt tcgtttgacg agggttcatt actgttttca
     2101 gaaaccgcaa ctgtttcttt tgtttgcttc accggacgtc tctgcattgc caaaatggct
     2161 ttttgcagtt ctttttcaga tactttttgt tcactaaagt cataagattg actgatcagt
     2221 tcgccaagtg tcaattcatc gaaaccgtaa agctgatgat acatgtcgac ttgtttgatg
     2281 attggatcat caagacttgc aatatgtttt tttgccagcc aatcattcaa aaactgccaa
     2341 tcgaatgtcg aatctgcttc aagtaatgct actgattcag attcttcaaa agatttattc
     2401 agcgattcga tgttttcttg attagcagcg tacactgctt cattgaagcg ataaacatct
     2461 aaaaattttt ttgtgatttt ttgatatccc tctgctgtat gccttttagg ttcaaagaat
     2521 tgggtaagct tctggaatcg tcgttcacca actttttcca gaagaagaaa agccagaaca
     2581 gagtctttga aaaatgcttt aggaatgagt ggttctaaca gctcataaat aaaacgaggt
     2641 cccagatcac tatcttcttt aaaataaaca tccagtaagc cgattccttc tagctttttt
     2701 cttgcatcat aaaattgcgg gataccgcag cctagactac taagcagatc tgcatgcatc
     2761 ccttcaggac ctttgccaga ttcttgagag atttctgata acagggtgaa atagatactc
     2821 aaggcttctc cgccgattag tggctgataa agatacagca aggcgttctt gctgtcttct
     2881 ctcagcggag ccgcttttct tccttggtaa atattttttg gttgaagctc tttccaagca
     2941 ttttccaagg aaatgcctcc tttttgtttc ttaattgttt tctttattat tctctttatt
     3001 attttctttt gctttatcca cgatatcttg caactctttt aagaaaacag acatatcttt
     3061 gaactggcga taaacacttg caaaacgaat ataagcgatt tcgtctaaat caactaaatc
     3121 ttccatcaca tattcaccaa ttagattcgt cgatacttcg ttttctccga gactgcgtac
     3181 gcggttttcg acatggttga cgatttgttc catttgttcc atagctacag gtcttttttc
     3241 tgctgaacgg atgagtcctc ttaaaatttt atcgcgattg aactcttcgc gttccccgtt
     3301 tttcttgatt actaataacg gagcagcttc aattcgttca aatgtagtga atcggaacga
     3361 acaattctca cattcgcgac ggcgacggat cgcacgtcca tcatctgctt gacggctgtc
     3421 gattactcgt gaattattat gttgacatct gggacatctc attttgtctc acctcttgca
     3481 tcgcatttta tataaaggat gtgtctttct ttacaactag ttgtattata acacgaaaca
     3541 aatcacaaac gcgactgtcc tacccaaaaa acacggatat aagaacaatt ttttctactt
     3601 tcatgcgtaa ttgtgtcccc tgtccttact aaatttcaat tttccaatac tgatcacctt
     3661 gattactgag aaataactta cacaaaagct gatttagtca tctagcacaa aaaacttgaa
     3721 gcagatgaac accttctcat ctgcttcaag tcacatctcg attttttact attttataac
     3781 ttctcttcga ttcctgtata gtgtttcatt cgctgttcct cgattttttg ctcatcggga
     3841 atgtggtcac tttctgggag cggtgtttta ctacgatcag gtgtcggatt ttttcgttct
     3901 tgagtcaaat cttgccgctt cgcttcttct tctgcctgct ggtcttctat ttcatcgatt
     3961 cttgcttggt actgcttttc tttttcttct gctgtttctg attgcgtacg accttcatcg
     4021 attagatttt ctttattgag catctcgccg attttttctt ttgcgttacc tacgacttta
     4081 tccttttgat tttttaatga tcccatcgct atccctcact ttctatatac tcaggatagc
     4141 aaaattgaca cagatactca aagaaagcga ttagcaaaaa gccaattttc gatttgtccc
     4201 acaagttttt cttttgttcc attgttgtcg aacaaaatat ccgcacgctg ctttttttct
     4261 tcgattggaa gttgactact aattctctgc tgcgcttgtt ctagcgtcag ttgattacgt
     4321 ttcatcaaac gctgaagctg agtttctggc gtagtataaa cgacagctac tgcatccata
     4381 tagtggtcat aatgaccttc gtagagcaat gggatatcta caattactaa aggatgattg
     4441 gaacttagag aggcgatttg atcttttatc gcttttcgga taaacggatc taatagctga
     4501 tcaagttttt gccgtttttc ctttgatgga aaaacaatca tcccaagttt ccctcgatct
     4561 agttgcccat caggctgcaa tacctcttgg ccaaacactg atacaatttt cctcagagca
     4621 agcgttccag gctcaacagt ttctctggca atcacatcgc catcgatcaa aggaaatcca
     4681 tgagactgaa agactttggc tgccgtactt ttaccggtag ctattccacc cgtcaaacct
     4741 aaaacaaatc ctactttgcg atttttagcc tttgacaatt cggacaatag tgcgtccccc
     4801 tttgtgccac ttttgttttc acgatcggcg ttccacaacg tatacatggt ttgccagttt
     4861 gctgatagac atgcagtgct acttgaaatc cgcctgattc tcctaatgca ttcaaataac
     4921 tgcgaatcgt ggtacctccg gcttctattg cacgatccaa aacatcaatg atcgatagac
     4981 gtaattgttc tacctctttg gggcgcagtg tgttcgccgg ttgttctgga tggatcttcg
     5041 cttcccataa tgcttcatct acataaatat tacctagacc agtcactaga cgttggtcta
     5101 gcagcaatgg cttgattgct ttgtgggact ttttcagtcc atttgcgaaa tctgctaata
     5161 agaagctatc tggcaaaggc tcaggtccca atttcatgat tcctcggtaa gttgcacttg
     5221 ctcctttttc gacgattgtc atacgaccaa attttcgtac atcgttgtag cgaagttggc
     5281 ttccatcctc aaaaaagaaa agtacatgcg tatgtttatc gattggtgta ttttctttcg
     5341 taaattgata ttttccttcc attcgcaagt gtgagatcag ttcacaatga tcgagatgaa
     5401 aaattaaaaa cttccctcta cgtccaatgg attggatggt ttcgccaaca agagaagcct
     5461 caaagatggg tgtctctggt tgctcgatga tcttgggcca caagacctga actttttgaa
     5521 tcgtttttcc tactactaac cgttccaatc ctttacgaac ggtttctact tctggtaatt
     5581 ctggcatttt ttcttttcac cttcattata ttgctctgtc ttaaacaatg tttattttgc
     5641 ttcgtaccac gtttttcccc aactgctgtc agtgatcagt ggtacatgaa gagaaacagc
     5701 ttgttccata acttccttca ccaatttatc taatgattca agttcttttt ccggtacctc
     5761 gaagacaagt tcatcatgaa cttgtaacag catcgtagct tgaagacctg tttcttttaa
     5821 tcgtttatcc aattcgatca tcgcgatttt caaaatatca gcagcacttc cttggattgg
     5881 tgtattgata gctgttcgtt ccgcaaaaga acggatattg taatttcttg aattgatgtc
     5941 tggcagatat cgacggcggt gatacagcgt ttcgacgtag ccttggtctt ttgcttcgcg
     6001 tacgattctt tccatatatt ctttgacacc tgggtatttt tcaaagtacg tatcgatgta
     6061 ttgctgtgct gcttttcggc tgattcctag attttgtgaa agtccgtaat cagagatgcc
     6121 atacacgata ccaaaattca ctgcttttgc ctgcctacgc atatttggtg tgacatcctc
     6181 tgccttttcg attccaaata cacgcatcgc tgtgctggca tggatatctt gaccttctag
     6241 gaaggcagct ttcaaatgtt catcatttga gatatgcgca agtacgcgta actcgatttg
     6301 tgaatagtca gaagaataaa tcagccagtc tttttctcta ggaacaaatg cctgacgtat
     6361 ctttctccct tcatctaatc gaatcgggat gttctgcaaa ttaggatcaa cagagcttaa
     6421 tcgtcctgtt tgggtcaatg tctggacata acgggtatgc acttttccat ctgattggat
     6481 cactttcagc agtccctcga cataggtaga ttgaattttt gcaatttgac gataggtgag
     6541 gatatcctct acgattggtg cttgttcacg aagctgttcc agtacatcta cggctgtgga
     6601 atatccagtt tttgtttttt tgataacggg taatcccatt ttttcaaata agatcactcc
     6661 taattgcttt ggtgaattca aattgaattc ttcgccggct tcttcgtaga ttttttgttc
     6721 gatctctctt aagcgagcag agaactcgcc cttcatttct tgtaagcgag tcgcatctac
     6781 tttgatcccg cttatttcca tctctgccaa aatgatagac aatggcagtt ccattttccg
     6841 gaaaagatct tcttggtttt tttctgccag ttcttggctc aattttggtg tcaacgcatt
     6901 gattgcagct acttttcgcg ctaaatgagc aaaaaagagt tcctcttctt ctggtaaacc
     6961 ttttttcgcc cctttgccgt atacagcttc atctgattgg atatcgttgt atccgtaatg
     7021 agcagctatt ccttcgatat ctgtgctttt ttcattcgta tcgagaaggt atgcacctag
     7081 caagacatca aaatcgattc ctttcgtttt tccagtatag cggtttaaag caacataagt
     7141 tcgtttcgca tcatatactt ttttcagccg agtcggatca gtgatccaag aatggaacgc
     7201 tcggttttcg aatacagata gatcgttcgt gacataaatt ttcttttctg tcccccacgc
     7261 tactccgacg atttcctctg tatgataatt gtcgcccatc atttccacgt acaacgccat
     7321 atccgtagta aacatttcct ctttgaattc atgaacgact tcaaacaaaa tatcatccat
     7381 ttttacttct tcttctacga tattcaattt agaaaggaac tgattgaaat ccatttcttt
     7441 ataaaacgga acgagtttat cgagattttt cccttcatac ttcaatgagt cgatagagac
     7501 atccacaggc gctgaagtat tgatggtcgc caattttttc gataagaaag cctgctcttt
     7561 gtcatttatc agattttctt tcattttgct ttgcttcatt tcatcgatat gttcataaat
     7621 tccttcgact gagccatatt gtttcaatag cttgatagct gttttttcac cgattttagt
     7681 cacaccgggg atattgtcag aggcatctcc ggctagtccc ttcatgtcaa taatctgttt
     7741 tggcgtcaac ccatcatatt tttccgctac atgttctggt gtatagcttt cgatatcgct
     7801 gacacctttt acagtgatat ccacttttac ttctttacta gctaattgag tcaaatcccg
     7861 atcacctgaa agcacgacga catcaaattg atctttgtct actttttctg ccaaagtacc
     7921 gataatatca tctgcttcat aattgggcaa ttcgtaatac tgtaccccta gtcctgtcaa
     7981 caaatcacgt atatatggca tttgttcttt gaattcgcca ggtgttttgg atcttccacc
     8041 tttgtactcc gcatacattt ctgtccgaaa agtcgttttt cctgcatcga acgcaaccag
     8101 aacatgagtc ggttgctctt tttgcataac attttcaaac atcgtattga atgcatagat
     8161 tgcatttgta tgaagaccgt ttctattttt gaaacgttca agagaattgt gaagcgcaaa
     8221 gaatgcgcga aaagccacac tgttcccatc aacgagtaat aatttatttt ttgtcatctt
     8281 cttaactcct tactgtaaag ttcttattca ttctaacaaa gaatcctgcg gaatgcgaaa
     8341 agaaaaagcg ggaccatacc aaataattcg gtatagcccc aactgatgaa tttttttctt
     8401 ttttagttgt tccgtccaaa gattctcaga aacgccaaga acaagttgat aaagtccaaa
     8461 tacagcttca atgccatgaa tacagcaatt ccactatgtg ctgttccgtt tgtctggaca
     8521 tagatattac ggatgctttg gttatcataa gctgtaatcc ctgcaaagat gacgactaac
     8581 agtagggaga tgaacagctc aactacgcta cttctcaaaa tgaagatgtt caagactagt
     8641 gcaatgatca ctccgattaa agctgcctgt cctgctcttc caaccccgct aagatctctt
     8701 tttgttgtta ctccgacaac agacatcccc atgaacatca aagtagctgt caagaaagct
     8761 tgcgttactg ttccaatatc gtagtatgca agagtaaccg ctaatgttat gccattcaaa
     8821 agagagtagg caataaatcc accgattgct aagctcggat tcttttgtgc ttttacgcct
     8881 agaaccatta ctaaaatcag ttcaacgatc cacagaccca caaagcctag tggaaaatta
     8941 ttgataaaag ttgctaattg caaaggaaat acccaaagag ccaagtaaga agtgactgca
     9001 ctaagggcaa gtcccatacc taaaaatcca taaacttttg cataaaaacg attgatacct
     9061 gacattgatg tacttggatt attcattcaa ttcaccttca ctttcgattg ctatttccaa
     9121 ttcaggaact tgtggcggtt ggaccataac gacagcatcc aacacacgat cttcgttcgt
     9181 attctccgct tctacagaaa tcgtcaccac atccttcatc ttatctactt ttatgacctc
     9241 aaactgaaaa gtcagtgttt cataatggaa aaccggttca ataaaattga cagaaaaatt
     9301 gacaacatgt gatcctggtc caggaagatg ctttgaaatt gcacttgtga tgatccccat
     9361 cagcatcact gatggaacga ttggctgttg atattctgtt ttttgtgcaa agtcatgttg
     9421 gatatacaaa ggatttgcat cattcgtcaa tcccaaatac agcaaaagct ggttatcttc
     9481 aatagattct gttaaggata acgagtcgcc ttcctcgatt tcatttattg tttttcctag
     9541 ttttctcggc ttacctatgt tcaagataga aaccctccag tttattttta atcatacatt
     9601 attgtaccaa aagtagcgta aaaggcaagt tctgctccct tgatttaaca gattcctata
     9661 gtttacagag gtcgtgaaaa agctgtcctg catcaaataa taagaacagc caagaaaaaa
     9721 cagcttctca tgtttttgat ttgattgggc ttattaccgc agatgcaagc ttttgaacac
     9781 cgtttatcat aagttgcaaa aaagggggct tggcaagatt tttgccactc cccctctttc
     9841 tatttttata aggacactgc agtaattgta ttacagtaca tttttcgtcg tgctgcttaa
     9901 aagtttttca tacgcttgtt caaatttttg aacatctcca gcacccatga agattactac
     9961 tgcatcatga taatctaaca atggagatac attattttct ttgatgactt ctccaccttt
    10021 tttgattttc gctccaaggt cttcaatctt aacgtttcct tgttcttccc gagccgaacc
    10081 aaaaatatcg cataagtaaa ctttatcagc taaatcaagt gcttctgcaa attcatccat
    10141 caatgcaatc gtacgcgtaa acgtatgggg ttggaaaact gcaattattt ctttatctgg
    10201 atatttttgg cgtgcgccat caatcgttgc tttgatctct gcagggtgat gcgcataatc
    10261 atcgactacg gtcatgtctg ctacgatctt ttcactgaat cgacgtttta ctcctgggaa
    10321 agtcagcatt tcttctgcaa cttctttcag atccagtttt tcgaaataag ctactgcaat
    10381 aacacctaaa gcatttaaga tattgtgttt accgaatgct ggtacagtga agtgcccaac
    10441 aaattcatcc ccatgataga catcaaaagc agaaccagaa gttgtgcgtt cgatgtttcg
    10501 tgcttggata tcatcgtttt ctgtgacacc ataataataa atcggcacat ttgcttttaa
    10561 tttgcgtaaa taagcatcgt caccatacgc aaaaatagct tttttcactt gtccagccat
    10621 tgtttggaaa gctgtaaata catcgtcgat acttgtataa taatccggat gatcaaaatc
    10681 gatattggtc atgattgcat agtctggtga ataggcaagg aaatgacggc gatattcaca
    10741 ggcctcaaat gaaaagaatt ccgcttgcgg atcaccatgt cctgttccat ctccaattag
    10801 ataacttgtt ggacggacac cactcaatac atgagagagc agtccagtcg tacttgtttt
    10861 tccgtgagag cctgttacag cgatactcgt ataattttgg ataaaatgac caataaagtc
    10921 gtgatagcga atcacttcca aacctaattc ttttgctcgc tggatttctt catgtgaatc
    10981 aggaaatgca tttcctgcaa tgatcgtcat ccctggtttt acattatctg cgttaaatgg
    11041 cagaatcgtg atatttgctt tttctaaatc tctttgtgta aagaaatatt tttcaatgtc
    11101 tgatccttgg acatttagac cttgttcatg taaaacaaga gccaaggagc tcattcctga
    11161 acctttgata ccaacaaaat gatacaattt attttgattt tccatttgaa tcctcttctc
    11221 ttatctagcg tccattcttt tagctattac attcaacacg ctcctcatta tcttataaac
    11281 gattgaaaat ttcaaccaaa tttatttgga aaggaggatt cgcttgctaa aatggtcaag
    11341 tacgagaaaa atccgttttt attgactctt taattctggt gtaagaagac caaacttcct
    11401 ctgatatatc aatgtgagga ataaaatctt ttcctttcgg accatagccg ttctcatcgt
    11461 catggagacg ttccatttct cctaatacta gcgcatggaa atattcctta ggccattgat
    11521 gattcagttc ttttgctgta aagatgatct cttcagaaaa gtgaaggatt tccggcacaa
    11581 atggcacagc attcgcgaaa atagtttgtt cattttgcca tactttctca aaagttgctc
    11641 gagtcctgcg ttgcagtgtc ggatcattca tcaataagat tttttctggt acagctccta
    11701 aggaatgaag aatctccagt gaaaaagtgg cgttctctcc agaatttgta gacttcgatt
    11761 ctatcagaaa agccttatcg ggcaaatcat agacttcttt tagatactga cgacagattt
    11821 cactttcact catgccatct tcaaaatgaa atccttgctt ttcaaaattc tcataaagga
    11881 ttctagtcgc atgccccacc cctccgacca aaaacaattg atctacttgg ccatttaaat
    11941 acatttgtgc tgctttatca gctaagatag gaagacagtt tccagctaaa ataagtgtag
    12001 gtgccgtcag ttcctcaaaa tttccctctt caggtgcggc taaaaaattt aatagctgat
    12061 tccagtcttt tagattcctc tgctcacttt tttccaaagg ccttctatcc tttcatctga
    12121 ttttttgtgc ttcattaaag tcttctcttt ttgtctcaat ccatttgatt tctccatgac
    12181 atcttccaca acgatatttg cgtacatcga ttcttctctt gcggcagatc gtggcagaac
    12241 aatcttggca tcgataatac tccatcttta tctgtttctt cgtcaaggct ggtgcatagc
    12301 gcaaccctcc tgtttttttc aaaagatact tgaaatccat atctttatgc tgataccctc
    12361 ttccagctag atgaagatga taatggcaaa gttcatgttt gatgattcct gcaatcgtct
    12421 cttcgtctga ctcttcaaat agcttcgcat taaaatctaa atgatgatcg tttaaatgat
    12481 agcgcccgcc agttgtacgt aagcgtggat tgaacattgc ttggtgcttg aaaggaagct
    12541 ggaagctttt taatgatatc tcttctacta actgctgcag ttcttcttgt gtcaattttt
    12601 gttactcctc attgttactc ttcataaggg gaaagcatcg tcaaactgat tctgcctttt
    12661 ttcgtatcga cttgttctat ccaaactgtt acgatatctc ctactgacac gacatctgta
    12721 ggatgcttga cgaatttttt gctgagtttc gatatatgga caagtccgtc ttgcttcaca
    12781 ccgatatcaa caaatgcgcc gaagtcaatg acattacgga ccgttccagt caattccatc
    12841 cctggtttca agtcttccat actcaataca tctgtacgta ataagggcgc tggcatctca
    12901 tcacgcatat ctcgtccagg ttgtgtcagc ccagctaaaa tatcggctaa cgtttcttct
    12961 ccgactgata ggctttcagc cagtttctct acggataatc gtgtcaattt ttctactgct
    13021 tcttctgtac caagctcttt ttctgaaagt tggactgaag ttaatatttc ttttgcaata
    13081 gaataacttt cgggatggat acctgtgttg tccaaaatgt ttttcccacc agggactcgc
    13141 aagaatccga ttgcttgttc atatgctttt ggtcctaagc gtggcacttt tttcaattga
    13201 gttcgtgcgg tgaattctcc gttttcttca cgataaatga caatgttttg ggcagttgtt
    13261 ttattcaatc ctgatatatg ctgcaagagc tgcgggcttg ctgtattcac atccacacct
    13321 acttggttaa ccgctgtttc cacaacaaaa tccaattgtt cagccaaacg tttttgagaa
    13381 acgtcatgtt ggtactggcc tactccgaca gctttaggat cgattttgac taattcagct
    13441 aaaggatctt gcaggcgacg ggcaatagaa acagcacttc tttcttcaac ttgtagatgc
    13501 ggaaattctt ttcgtgcaat ttcgctagcg gagtaaacag aagcgcctgc ttcgttgacg
    13561 atagcgtaat aggttttatg gtcggcggat ttcaactgct cagccacaaa aagttcagat
    13621 tcccgactgg ctgtaccatt tccgatagct accatatcta cttgatattt attaattaat
    13681 tggataaaag ctggtccagc agcttctctt tttgcttgcg ctgcaggttt atgaggataa
    13741 ataacttcga ttgctaaaac tttccctgtc gcatctacga ctgctagttt acatccagta
    13801 cgataagccg gatcaaatcc caggacaact ttacctttta gtggcggttg caacaaaaga
    13861 ttacggaggt tttcgccgaa aatagcgatt gcttgctcat ctgccttttc agtcaattca
    13921 ttgcgcagtt ctcgctcgat tgctggctga ataaagcgtt tataactatc ctgataggct
    13981 tctctgacga atgaagaaga aggagaggca ggatttttga ctatttggcg atccaagtaa
    14041 gctaaaattg ctgcttcatc tacttgaaag aatactttca gaatatcttc tttttctcca
    14101 cgattagtag ctaatattcg atgagaaacc attttatgga cgggttctgc aaagtcataa
    14161 tacatttcat atacgccttt ttcatctgct tgctcatcct tgacattgct gacatacata
    14221 cctttgttat acgtataaga ccgaatccaa gtccgaaatt ttgcattatc gctgacttgt
    14281 tcagcaataa tctcatgagc accgtgcaac gcttcttcgg cagaagagac ttctttttct
    14341 ttgtcaatat atttttctgc ttcagattgc acctctccat ctgtaagctg catcagccac
    14401 ttcgcgagtg gttcaagtcc tttttcttta gcaatcgttg cttttgtccg tcgtttttgt
    14461 ttgtatggac gatacaaatc ttccacttgt tgcattttca cggcttgggt gatttctgct
    14521 tctaactcgg gagtcagttt cccttgttca tcgattagcc gaatcacttc agttttacgt
    14581 ttttctaagt tttcaagata ggcataccgc tcttcgattt cacgaatctg gacttcatca
    14641 agacttccag tcatttcttt tcggtaacgc gcgataaaag gaacagtgtt tccttcattt
    14701 aacaaattaa gtacagtagt caactgctta ggacgatagt ctgaaagttc tttttgtaca
    14761 agctgaatga tcgtctggtt caaattttcc gtcattaaaa tgcctctttt ctgctaaaaa
    14821 ttgcttcttt attctatcat actttttatc ctgggttaaa ctactgaaag aaacaatatt
    14881 ctctataaaa ataaaaagag ccgcaacaaa gtcacagctc cttcattctt tccattagtc
    14941 agattccatg ccaaggtcct tcgttgttct gatattcaat atcttgaaga ataccttcct
    15001 tgtcaaaaac aaccccatgc aatgaccctc cgaatacagc cccaccgtcc attccgattt
    15061 tatgatcctg aatccagagg tctgttgtct gcatatctcc atggagcatt ggtgtaatcg
    15121 tatgtccaaa tacaattgtc ttacctgtat tgtttttccc ttcatgaaat ggttccctta
    15181 tccaaataaa gtctcttaga cttgtcttac gccagtcttt ctttgtcaaa tccacaccgg
    15241 catggacaaa aagataattt tcccattcat aatacaaggg gcgctggact aaaaatttaa
    15301 tcagttctga atatctcgat tggatcatca tcgagatttc agttggggag tattcctccg
    15361 atgcccctgg gtgaatcagg ctttcaaacg tttcttttcc accatttctc atataaggcg
    15421 cataccagtc ctccggtgat tgcagaaatt gcaaaaaata ttcttcatgg tttccccgga
    15481 ggtaaatggc tcctgtttct tcgactaatt gctttcctaa taaaaaacat tcttttgtac
    15541 gaggacctcg atcattgaga tcccctaata aaacaagctg atggatcttt ggatcataat
    15601 agtgaataag tttttcaaaa agctcatgtt tgccatggac atccccaata gcataaatca
    15661 atgatttcat gtggatcacc atcctcaacg ctagtatagc gcatattgga aatggtcgac
    15721 aatcaaaact attgttttct atttaattaa agctgtaaac cgcagttctg cttcttctgg
    15781 agagattcca tggtcttgaa catagcgatt gcgcggatgt ttcgcacact ctatgctaca
    15841 tgctcctaaa tgtttttctt cattttcaat ggaagcaaga atctgacgat tgcactcagg
    15901 attgctacaa ttgatatatc gttcacatgg tgttccgtca aaccagtcgc gaccgacgat
    15961 ttgtttatct acttggttga tatcgacagc gatccgttca tcaaacacat acatcttgcc
    16021 gtcccataat tctccttgga cttcaggatc cttaccataa gtagcaatac cgccatgcag
    16081 ctgtccgaca tctttgaatc cttcacggac aagccaacca gaaaacttct cgcaacgaat
    16141 gccaccagta cagtaggtca ctacccgttt ttccataaat tcttctttgt gctcgcggat
    16201 ccagtctgga agctctcgaa aactgcgtat atctgggcgt acagcccctc gaaaatgtcc
    16261 caagtcatat tcataatcat tccgtgcatc aatcaccact gtctgttcat ctaaaatagc
    16321 ttctttaaat tcttgcggtg aaagataggc acctgtcact tggtttggat caatgtcctc
    16381 ttctagcttt aaacttacaa gctctggacg gtatcttaca aacatcttct taaaggcatg
    16441 tccttcgctt ggatcgatct tgaacatcgt atcagcaaat cgaggatcag ctttcattgc
    16501 tttcatatag gcagtcgtgt tttcaacggt tccagacaca gtaccattta ttccttcttc
    16561 agctactaga atacgacctt taagatccaa agatttgcaa aaggctaaat gatttttggc
    16621 aaattcctct ggttcttcga tttttgtata attgtaataa agcagtactt gataatccat
    16681 gtatttcttc cttccttctt ttcgttccta aacagtataa agaaggcgtt cgcatttgac
    16741 gatttatttg cttgtgtata attacgcatg gaaagttttt tgtttacata atattattat
    16801 ttgaattaaa aagacttcgc tttcctgttt cgcacagaaa aacaaagtct ttatttctat
    16861 tatttacctg taaccgcaaa gtagttgcca tcgggatcag caaagttaaa gacatattct
    16921 tctccaagct gtaccatatc acctaatgtg actgattgcg tctgcatttt tttatatagt
    16981 ccaaagatat ctttactgta aaacatcaaa gatggggcac tagtagctgt gccaggtgaa
    17041 tgggactcaa caaatgctcg atcatataac agaaatgagg tttgactgtc ctcaactcct
    17101 acttcaatta taagcgtccc atccatttct tgacgatctt tttccgcaaa gccaactgct
    17161 ctccaaaacg ttgcattttt ttctacatcg tccacataaa gcatgatttt tatttcattc
    17221 gtaaacatct gtttcctcct aaacgatctg ttctaacatt ctcaactttg tcagtttacc
    17281 ataggaaaat gggaaaatct acgaaagctc tcaacagaat cgtcctactt aaatacgctt
    17341 ttctttaata ataaataagt tggttgtttt tgttatttgc gctttcactt ttcggaataa
    17401 ataatgtgta tttatatgga ataaataaaa aaagaggtcg tgactaaaaa caagtcacaa
    17461 cctctggtaa acggtgttct cctgacaact cctcggtcac aagacacaat attcaacaaa
    17521 catgagaagc tattttttga aattgctctt tgtgactcag agtgaaacgg caggctcacg
    17581 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taataagctc aaccaaacgt
    17641 aaaatatgag gaactatttt tgtttggctg ttcttattac ttgatgcagg gcagcttttt
    17701 cacaacctct tattcacggt gtttcagaag tagcttctcg gaaataagcc gaaatttcac
    17761 aaaaatttga gaaactattt ttaggaaact tcttcttatt tcttaaagct gaccacttct
    17821 atcacaacct ctttttgact atgcattcac atcgatcact ttagaatcat cattgatgac
    17881 atcgcgatct aaatctttga tgcgtgcact tgtcacgata ccagctacca tgctaccgct
    17941 gacgtttact gctgtacgtg ccatatcgat taatggttca acagagataa ctaacccaac
    18001 gattgctact ggcaagttca ttgcccctaa aacgatcagt gaagcaaatg ttgcgccccc
    18061 accgactcca gctacaccaa aagaactgat tgttacaatt gccacaagca tcaaaataaa
    18121 ttccaaacta aagacattca ctccagctgc tggtgcgacg atcgttgcca acatagctgg
    18181 ataaacgcca gcacagccgt tttgtccaat agacaaacca aaacttgctg agaaattagc
    18241 tgttgcatca tctactccta aagcttttgt ctgagtctcg atattcaatg gcaaagctcc
    18301 agcacttgaa cgtgaagtaa acgcaaagct caataccgga aaggcttttt tgaaatagtt
    18361 gattggattg acttttgcac caatcaaaat cagtgagtgg acaatcaaaa cagcaagcaa
    18421 tgctacgtaa gaagcaacga caaatttacc taaattgacg atagctgcaa aatcacttgt
    18481 tgccaaaacg tttgtcatca aagcaaatac tccataaggt gtcaaacgca aaacaagcgt
    18541 cacgatccgc ataatgattt tatacagact gtcgactaaa tctgaaaaga atgctgcttc
    18601 ttttggcgct tttcttttca cgcctagata agcgattccg acaaatgccg cgaaaataac
    18661 gactccaatc gtacttgttg cacgactacc tgctaaatca gcaaaaacgt ttttagggat
    18721 aaaggataaa atctgctgtg gaatcgataa gttttcaact gtttcctgac gtgttgccag
    18781 ttcagagatt cgtgctgttt ctgcagctcc ttgtgtgaat ttagctcctt ggagtccgaa
    18841 cagcattgtt gaaccaattc cgatcaatgc tgcaatagct gttgttccaa gtaaagaagt
    18901 caatacagtc gcactaattt tccctagctt ttcagaaact ttcatttttg tgaatgcacc
    18961 aatgatcgaa ataaacacta aaggcataac tagcatttgc aagaatgata catatccttg
    19021 acctacaatc ccgatccatt ccattgattg agtaacgact ttgtcgtcta caccaaagat
    19081 caattgaagc agaccgccaa agacgatacc aactcctaat gctgtaaaga cgcgtgttga
    19141 aaattttgta tggcgctttt gcatacgata gaacacatat aataatgccg caaaaactag
    19201 aaccactaaa actgtgatga gtgttgtcat ttttctcctc ctcatattat taatgacaag
    19261 tccatcgaag gaagtaaagg ttcccgagaa tgattcttca accatcctcc tgaaaaaatt
    19321 tttttcagtg acttttatat tttagacgat ttttatcgaa atgacattct ttttgcttac
    19381 aattagtaat aaaaaaacat acctgtgata gctttaatct atcgcaggta tgtttttact
    19441 gtctattctt gtttttctga ttgaaaattc aattttaccc caggctgatc taaggcgatc
    19501 tctgtcacct cataagacat tcggcgtacg atatcgaata acggtgctac tagctcgtcc
    19561 acttcttctt gtgtgacatc attttgacct tcaattttcc gattgaggat atgattgatc
    19621 tgactcacac ttccagataa aacatatgta tcaaacacta aacggaactc taagcgtgct
    19681 cctacgatgc tatgttcttt cggatagttt tcgatcgttt cttttaaagg cgagaagcct
    19741 actttcaaat ctgtttctac ttcttggttc ggcaatctca tatcataatg aaatgcttct
    19801 acaacttctt tttgtcttct gatttccata taattttatc actcctttat gaaaattata
    19861 acatatctcc tttgaaaggg gatactactt tttaatgatg ctctgcaagg taaggatttc
    19921 attcagattt cctaccacat aatctgccgg gatctcaccc agaaaatgat cgatgtcgta
    19981 aagacaagta gcgactcctg catttttccc tgcttcgata tctaaacgac gatcgccgat
    20041 catcatcgtg tctgttcttt ctaaatgaaa agtatcgatc aaataattta aagatgctgg
    20101 atcaggttta cgaggaaagt cctgatcgat ccctactact tcctcaatca gatgtgccaa
    20161 tcgatgttct tttaacaggc cccatgttga ttctgtcaaa cggtgggtca ggatgaaatg
    20221 acgaccgccg ttgtctttta gcatttccag tacttgtttc gtttctggaa atggacggga
    20281 aacttccgta ctttcttttt catatcggtg aaataattca ttaaactctc tggcattcaa
    20341 gtttgattca ttgatcaaat aactagtaga atactttttc attttgaagt agatttcttt
    20401 tttgtccata ctgataccaa aatcttttag tgcttgccat gcaccatcta ccattgcggg
    20461 atacgtatca aacaatgtac catcaaagtc ccatatataa tttttcatgt tccttttgtt
    20521 ccattccttt gcttcttttt tctatctctc acttttagat aaaggtgttc tttaaagtat
    20581 ttttttgtcc agaattgtca aatcatctgc taatgtatac tggattggtt gtgcatttgg
    20641 gatatcgatc gtatccattt gatcttcagg aacatcttct aaatattttg tcagtgctcg
    20701 gatgctatta ccatgaccca caactagtaa atcttgccga tcttttagtt ttggtgctag
    20761 atgatcttgc cataagggaa tgatccgatt cactgtcatt tccagacttt ctccgtatgg
    20821 gatcaaatgc ggatccaagt gatcatatct gcggtcaaat ctattttctg cggttaacgg
    20881 cggaacttcc gtatatcctc tgcgccatgt ttttacttga tcttttccgt atatttcttt
    20941 catcttatct ttgtttttac ccacaagatc cccataatgc cgttcattta gacgccatgt
    21001 ttttattata ggaagccata actgacccat ttcttctagt gcgacttgtc cggtaagaat
    21061 cgcacgtttc aaaacagagg tataaacggc atcgaattga agtccggctt caagcatttt
    21121 ttgaccggca actgctgctt gttcttttcc tttttctgtt aaggcgacat ctagccatcc
    21181 tgtccagtaa ttttcaaaat ttgcttcgct ttcgccatga cgtaataata ctaatttcat
    21241 tcttttctcc cttaaacatt cgtttcgaat tttataccac atgttttgca tgtcacacgt
    21301 attcgtcctt ttcctttagg tgcacgctgc ttttgatgac aattcgggca ctcaaaaaaa
    21361 gtataacttt ttcgatccat tttgacttgc tttttgtttt ttatctgatg atatttttct
    21421 ttgactctat caagatattt caaataacgt tgattctcat ttagtctggg gtagattttt
    21481 tttgatagga aacgaaatat catccatccg aaaaagacga tgaacaacaa ttgtccgatt
    21541 ctatagggca accaacgact tagcaaacta aagacaagcg cagcgatcaa tagaccgcga
    21601 ttcaactgat caaatttcgc atatcttccc tgcataagtt tgtgatagtt ctgctgaaat
    21661 cgtattagcc tttctaacca tgtttgcatc atattatctc ctccatcatc taatatttat
    21721 tatgacaaaa aaatccaaaa agaaaaagcc ttaattggcc ttttccattg caacttggtg
    21781 aaggacattc cgatttctgg taaagtcaat cacaatcact ttctttttat cttctctgtt
    21841 gactactcga aatgtgtccg tcaatggaag aaacagcttc ttagatggta gctgttgcat
    21901 acgtataaaa cgatggatca tcgtaatcaa ttgaggaaat tgaatagacg tgtgaagcag
    21961 acctggatat tttttcaaca gctgtgtcgt tttgatcaga ttccgttttt tactgatcac
    22021 ttttaaaaag ctttttagaa tcttttgctg acgtctaaaa atagaaaaag aattttcgtc
    22081 atctatttga taacgcattt gtttttgcag ttctgctgca gtcagatgga tcttcccatc
    22141 gtttgtatcg atcattaaac cttctggaaa cagttcaacc actgctcgat cccaagattc
    22201 aagagaaagc tcgatataat cttttactgg taattccaat tctaccttaa aaaagtgcaa
    22261 aacagccgac agcccttctg cttgatagat ttctcctaat gttagcccct ctagatggaa
    22321 ctctggtgaa aaatgcagca atgttttatt ttcctgcaat tgaacatata gaaatgtaga
    22381 tcgctcaatc agagtatctt ttaaaacaac taaaaaattc atttttctcc cccttttttc
    22441 atcattttta aaatagcata ttttgaaaag aaagtctgat gcttagagta gaatttcata
    22501 aaaatttact atttcataag tattcacgag aaaaatacaa acaaaaaagc ggaaataacg
    22561 ctcatccgcc ttcttgtttg tgtttatttc ctcttcaaac gcttgtctat gattcagact
    22621 tttctctatc gttattttca tctaaaagtt cttgcattcc gataatttct cgatattgat
    22681 gaatcttagt gataccaatg actttgtcta catcaatact aaaacaaata ccattacctg
    22741 gaatatcgat gttgatgcct tgaccaatcg catccattac tttattcgtc aaactatcgg
    22801 gtactaatgt caacacaata tctttttctg gcgaaatgtc caaacctaaa aatttccctg
    22861 aatcatgtac accagaacct cttccatgta agatcgttcc gcctgctgca cctgcttctt
    22921 ttgaaaagtc gataacatct tcgcccattc ctccgtcaac gatcgtcaca atcatgtgaa
    22981 gattcaatgc tacaagatct ttcatttgct ttcccccaat ttttgcaatc tttctttttc
    23041 gattctgtct ttttcatctt ttcgtttata aatatatcct agtactaaag tggataatat
    23101 tggtgtcaat gcaatcattg caaccacacc aaacccgtca atgatcggat ctcttccttc
    23161 aatttgtgca gcgattgcca cgctcattga taaaataaat gtcgaacaca tcgggcccgt
    23221 tgctactcca ccgttatcaa aagccattgc taaaaagatc ttgtccactt ttcgtcctaa
    23281 aataaatgct agcccgtatc ctgggatcaa gaaataataa agagagaatc cgacgagcaa
    23341 acgccacata gacaatccta ctgctgcgcc gatcccaatt gaaattacca tcagcatgat
    23401 tttcgatcgg attctcccct cgctcaattc ttcaacctgt ttgatcatta catgaatcgc
    23461 cggctcagca aaccctacta agaaccccat taaaaagcca aggggaacca gtatataatt
    23521 gtaaggcaat cccgcaatag cactgcctaa atgctgtcct accggtacat aagccatatt
    23581 gactccgtgc aggaaaagga tcaacccaat agtcgttatc aagaaacctt tcataatgtc
    23641 aaagaaacgc tttttgcgca tacgaaaact aatgatattc attaaaacaa ataggattac
    23701 gataggtgcc actgccaaaa tgacttccat ggtaacgatt tcgattccat ctatcacttg
    23761 ctgccagaaa ctcattggaa gatcaccccc aaaatcatca cggcaagaat cggccctaac
    23821 gaagcaatac cgaccattcc aaaactgtca ttttcatttt tcttttgttt gatcatacta
    23881 gtcatccctc cggcaagggc caagataaat ggaacagtta ctggaccagt agtcactccc
    23941 cccgaatcaa aagcaatcgg cataaattcg gaactggtga aaaatgaggc gatcaaaacg
    24001 cctatgtagc ctatagccat caattgataa taggatagct tgaatacgac ccgaagcagc
    24061 gcaaaggcta gaaatactcc agtgccgacg ctgacgatcc caatcagcaa cactctgcca
    24121 atctctccgt cagatatatc atatacttgt tgtcccaaaa cttgcacgga aggttcagca
    24181 atcgtgatca cgatcccgat agcaaatccc aaactgacca aaacggtcaa acttttcttt
    24241 ttgatcatat atttccctac tagatctccg acttccatca tagaataatc tgctccaaac
    24301 aaaaagagcg tcattccgat catcatgatt gccgcaccaa ccagaaatga agcgaggtct
    24361 tctccttcta acggtgcaaa aacaaacgtt aaaatgacaa tcaggacagt catcggcaat
    24421 atagcagcaa tcacttcttt aaaatttttt agcatgtatc ctcctccttt actctttaag
    24481 tttaccttaa aatgaaagag ttttcgagca caaacttaaa acgttatcat ttaatttttc
    24541 tcaacataaa ataacacacg tcccaaaagt atctttttac ttggaaaaat gactttgaag
    24601 gaacgcgctt attgtgtttt tttattttac agagccgatc attccttgaa cgaattgctt
    24661 ttgcaaaaca aaaaatacga tggcagttgg taaagtagcg atgactaatc cagccatgat
    24721 cattccatag tctggtgtat aggaagcacc catagcagat aaaacaagcg gaaccgtccg
    24781 tttatcagga gattgcaaag ctactaaggg ccataaataa ttgttccagc tactcatgaa
    24841 tgtgatgatt gcagccgcgg cataggtatt tttagccgtc ggcatataca ctctaaaaaa
    24901 aatacttagt tcttttagac cgtctaatcg agcggcttct actagatctt ttggaaaaga
    24961 tttaacattt tgtcgaaaga agaagatcaa aaaagcagtg ctaacagatg gcaggatgac
    25021 aaccaagaaa ctgttgattc ccaaaaccgt tccgttcaat tgtgaaaaca ttcgatataa
    25081 agggatcatc aatgccgcaa aaggaaccat catcgatagc agtagaatat tgaaaattag
    25141 gtcttttcgt ttactttgat aaatctcaaa tccatatcct gccattgacg aaatcagcaa
    25201 tgcaaacaca gtggtcacca aagcgatcat cgctgaattc caaagggaac gagtaaaatt
    25261 cagctcattg gaaaacaaat tttgaaaatt gatgatcaag ttactgccaa tctttatttt
    25321 tcccgatgtg atatccacgg gtgtattcgt cattcccaaa accatccaga caaaagggaa
    25381 aatggaaata aatgatagga tgcttaatgc tgtatacttt aatactgacc caattgtcga
    25441 acgaagttct aatgcttgtt ttttgacgtt ggggcgttcg attgtttctt tttctaccat
    25501 atttttcatc gactttctcc tcctactttc gtttggatta tcgacaatat cacaataaag
    25561 gaaacaataa caaaggaaac tgctgccgca tatccaaaat taggcgtgaa tttataactc
    25621 aaattataga tgtattgaga tagggtggtc gttgcatttg ctggtccccc gccagtaata
    25681 ttttgtactt catcaaaaag ctgcaatgtc ccgatcgtcg aagtaatgga agtaaacaaa
    25741 atgattggtt ttaaatttgg gatcgtcact ttgataaact gctgccattt agaagcaccg
    25801 tcaatagatg ctgcttcata aatctccgaa tcaatatttt gcatgcctga aaggaaaaaa
    25861 atcatattgt atccagtcca acgccaagtg atcgaaagaa tgatcagaac tctcgcccaa
    25921 attggatgag tcaaccagcc aatcggtgtg tcaatcatac caatgcccaa caacaagttg
    25981 tttacaaggc cagattgaga aaatagactt ttcatgatca gagaataact gaccatcgaa
    26041 gtgatacatg gaagaaagat agcggttcga aacaaccctt tatatttcaa ctttttatca
    26101 tttaaaaggt tggaaataaa aagtgctaga aacaacatga tcggtacttg tacaagtaaa
    26161 tacaggaatg tattaaaaaa agctttttta aaagttgcat ctgtcaacat ccgctgataa
    26221 ttacctaacc cgttaaatgt catattgaca gggggaccac tttgaaaaga cgtgatcaaa
    26281 gccaaaacca tcggaataaa aaccatgatt gatatcaaaa tgactggcaa gattaaaaaa
    26341 gcatgcccgt ttttgaataa atctttctgc ttcattcttt tccctccaat tctatcattg
    26401 attatctaca tgaaaagaca agaaagacaa tatctgtaag gcagacgtga tcttttttgt
    26461 cttttcgttt gtctgatgaa aaagcgaaga aattcacttt tttaccaccg atcaattagc
    26521 caattgtgct tctacttgtt tttgtgtatc tgctagtacc ttgtctgtct cttctccatt
    26581 gatgattcga tgaagagctt cagctacgac tgcttcaatt gcataggtct catgtccgta
    26641 attcacttct ggtatttcag atgtccatgt tgaaaaatcg tcaaagatct tttgaccact
    26701 gtaaaattca gaagcttctt gataagcttc ttgttcattc gcgctaagca tcgtagatac
    26761 tagtccgatt tctttcgcaa gagtgcccat cagttcttca ttcgaagcaa acgttgcctc
    26821 taggaaatct ttcgctgaat cttctccagc tactcctttg atcacatacc agccagcgcc
    26881 gccaaggtta gaagcttgcg ctttttgcag attttctgga agtgcaggta ttggtgcaat
    26941 cttccatttt ccagattggt tttctgctcc ttggatcgtt gaagaatacc aagcgccagt
    27001 tgggctagaa gctactgctc ctgattgtat cgcagttact ccagcattcc agtcagaggt
    27061 ttgttcgact agatcttctt tcaaaagagt ggcaaacaat tcaagaccat actttaaaga
    27121 tgcattgtct tggatcgtga ctgtctcccc atcttcagat gtataccatt ctcctgcctg
    27181 ttgcataatc atccttactc gaccaaggtc agaaggattg acttcagcga ttttctttcc
    27241 cgtcttttct ttgacatctc gcgctacttg aatatactcg tcccacttca gattctccat
    27301 atcctcttct gtataacctg cttctgccat cagatccgta cgatagaagt ttgctgttac
    27361 gcccgaatcg aaaggaacac cgtatatttt atctcctact ttgtttaccg caaatttata
    27421 cggtgcaaaa tgatcttcct ttacaatagc agacaaatta gcgaatgcat ctggataaga
    27481 agtcaaatag ccttgtatcc tataatcttc aatcaagacg atattgggca atccttctgt
    27541 attcccgctc gctaaagcgg tgttcagttt ctgaacgata tcatcctgag acatcgtaac
    27601 aacttctatc tctgtttctt cgttctcata aacttttttt gcttcattca ctgctttgat
    27661 attgaatgtc tcatcccacg cccagacagt gactttttct tctttgtctg ctgtactact
    27721 agcacttccg catcctgtta agaaacctga tgctgccgct aaacaaacga ctccagcaat
    27781 taactttctg atgttcattt ttatccgctc cttttccatc cattagttgt ttacgtttgc
    27841 tacattcatc ataccaaaat ttgaaaacgg tttctttatt attcagatac ttcaaaaatt
    27901 tctcctaaaa taataaaaaa atacaaatca actatgacaa aaggaataat cttagcatct
    27961 agaccaaaga tcgaaatggt gaggtttctt taaaataaaa atatccttgc attttctcct
    28021 agaaataagt atttttaaat tacaagacta ttcttaaggc gggtaaaaaa atgccaatac
    28081 gcgtaattct tatcgatgat gaacagacga ttttagatgg aatgaaacag ttgatcaatt
    28141 ggtctgagca cgggtttcaa ttaataggga ctttttccag tccgctgaaa gcgcttgatt
    28201 tttctttggt acatgcaatc gatatcgtgg tcacagatgt aatgatgcct gaattaaatg
    28261 gaatcgaatt atcgcggcta ttaaaacagc aacaacctga aacacagatc cttatcctct
    28321 ctagctatga tgaatttgat atggttaaag attcttttaa agaaggcgtc gctgattatc
    28381 ttttaaagcc tcgattgagt cctgattttt ttctaaattc cttaactgcc gtttcacaaa
    28441 aaatcaaaaa aaagcccaac aaaagcccaa tcaccaatcg ttatgagcag atctgcgaga
    28501 atctttcaca gcagattgct ggacaaactc gtccattgct tgaccctctt ctttttgaac
    28561 acaccttgtt tttctgttat ataccgaaaa acggccattc agttcaaaac aaggctctaa
    28621 acaaaaacag tcgatcaccc attcattgat ggatagcgac gattcgttta ctatttatcc
    28681 tgtttctacc ttttcggaag aatccggcta tgtaatcaat actgattcca aagatacact
    28741 taggaagttt gttcaaaagt ttcgtcaaga agtttttcca ggtgattttt ttgttgtttc
    28801 tgattctttt gcaatccaag atatcaatga aatattccaa gagatcagaa attgcaccaa
    28861 aggacaacgt ttctatcata agggaaagaa ccttgcttat cagcatgaac ttcttccgtt
    28921 attaaccaac cacgagcagc aagcaagtag ttatttaaca aaaatcgttc ataaagacta
    28981 cgctttagct gtcaaagaac ttgagaattt cttcacgacg atattggaac agcctattga
    29041 tccagcttat ttgaaacatg aggcaatcaa tcgtttctat gcattactca acgctttggc
    29101 tgacgagcat gttgggaatg aagaactttc tttgctcaag atcgcccttc cttctttatt
    29161 agccgatgct gagtatttcg aagatttttc tattcttgtc aaagaaacac ttgagaaact
    29221 cacccacttt tctgcaaatg caaaaaaaga caatttcgat atcttgactt tgatctacga
    29281 gttcgtccag gaaaattatg agaaagaaat caatttacaa cttttatccg aaaaatatca
    29341 tttttcatat agctatctct cagccatctt tactgaaaaa tttggtatca acttttcaaa
    29401 atatctaaaa aaagtccgta tcagtaaagc caagctcttt ttgacggaaa cacggcttac
    29461 tttgacggaa atctgtgaga aagtcggcta taccgaactg ggttatttct ccagagtttt
    29521 taaagaagag acaggaatga caccttctca ataccgtaaa ggaacttttg taaaatgaga
    29581 aaaaaaatca aagagcatga actctttgcc aaacttctgt tcattttgtt catcggtctt
    29641 tttctgcaag ccatcgtcat ttctttgttt atttatcatc gatcaaggga tgcctatatc
    29701 cagctgttca atcaatcgaa tgatgtggta ctaaaaaaga ttcaatctga atttgaaagt
    29761 ttgaatgata caatcgagaa tacattagcc gcttttgatt ctaatccagc tgtcaaaagc
    29821 tatttttcca atgatccagc ccaacatatg gaacagcttc agcaactaag aacaatccaa
    29881 aaaatgaatg actctctttc aaaaatccat ccaatgatcg attacgatgt attgatcttt
    29941 ggagaaaatg gaagaacttt tgtcggaaac gacatgctca ctgccgtatc tgccgattct
    30001 ttttttcaat ctgcgattgc tcagagagtg aatgaacgtg ctgctgatac acaaatgctt
    30061 tttgcacacc acggattgac tcttcgcgat aagaaagcac ccagcgtctt ctttgtcaaa
    30121 aaactgaaaa atacattgaa tcacgtttac ggatatgctg ttttatcgat ctcatccaag
    30181 caactagcga atctttttca atctgtagta aatcctgaga tatcgaagat ctccttcatg
    30241 aacgaagaac agacgatcat cgcatcaaat gaagaaaata caattgggag ccgatcaatg
    30301 attttcaaaa aactaacagt aggtgaaaca acgatttcaa atggacagca gttgacccgt
    30361 cttcctctgt accgccagga ttgtacatta ctcagtcaag tcgatcttca ttctttatct
    30421 aaccaaatgg gcgtgatttt tccgattatt ttgtataata tcgtgacact tgcttttgct
    30481 ggctcattgg tatttatgta tttggatcga cacacaaaat cgatctaccg cttgattgat
    30541 tcgctaaaac ggctagagga agatggctta aaagctactg ttcctgtaca aggaagccat
    30601 gaagtccaat tgctagggaa tacgatcaat caattgctaa gtaatgctca gcatcattat
    30661 gaacagacga tccaagacga aaagaagaaa cgttcgcttg aaatccaaac tatgcaagca
    30721 cagatacagc cccattttat ttataatacg ctgactagct tgaaattttt gatttggcag
    30781 aaaaaaacag atgaagcagt tcaaggaatc gacagtttca ttactttact aagaagtacg
    30841 attgggaaaa aagaagaagt gattccagtc aaggaagaaa tcaaaagcgt acaaagttac
    30901 atcgatcttt tgagtatgcg atatggcgaa agaatctcgg ctaaaataat gattcctgat
    30961 gaactgcagt cattatctat gccgaatatg atccttcaac caattatcga aaacgcctat
    31021 cttcatgctt ttcacaataa aacgcaagga ttcatcacgg tgtacggcaa aacaaaaaat
    31081 cagcttcttt tattcgaagt gattgataac ggcgatggct tcgatactga aaataccaaa
    31141 caaaaaaatg atcatttttc aggaatcggc atcagccatg tagatgagcg gatacgccta
    31201 atgtatggcg atgcatatgg cttaacgatc caatcagtga ttgggacagg aacgactgta
    31261 acgatttgtc taccgcttct tcctaaaaat caatcagaat atcaaaaata aagaatagcc
    31321 aagcgctgat ttttttattt taagttaaat aaatcaatca gtattcttga ctttcatcta
    31381 cgcgtctctt ctactgtcag cactggataa cagcccaata tttgactttc tcctccgagc
    31441 atcttgattt cttggagcgc attctctact aatgtcattg gacggtccaa caacaaatcg
    31501 atcacgaaaa aatattctcc caagctagtt ttcaatggtc gggattcaat cttacttaag
    31561 ttgatttccc gccagccaaa agctgccagc attttatgaa gcatccctgg ccgattagca
    31621 ggtagtgtca agatgacact catttttact ggagctgact gctggctggt catctttcgg
    31681 tcaccgacga tccaaaaacg tgtctggttc aactcattat cttggatatt ctcagctaag
    31741 atctccaatc caacatgctg tgctgtttct aatgaagcaa tcgctgctgc gtcttctttc
    31801 ggatgttctg ctacatacat tgctgcagca gttgttgatt ctgttgctac taaaggaaca
    31861 ttaggataat gcgtttctaa aaactgttgt gattgtgcta gtgcttgtgg atgtgaaagg
    31921 atttttgtaa tctttgttgc tggattgcct aaaagctgct gcttgatagg aagaacgatt
    31981 tcacttttaa cttcgacttc tggatgtttg gaaagaagat cgattgtgtg atgaacagct
    32041 ccttccaatg aattctctac aggaacgaca gccagatcga cttgtttgcg aaacaaagcg
    32101 cgcaaacaaa cagggatgga agcatacgca gtcaattgtt ctgtaggaaa tagctgacaa
    32161 gccgcctgat aagtgaatga actttctggc cctagatagc tgacgttcat atgcttttga
    32221 tcctttctat aatggtatcg atgatctttt ctggtgtttg attggtcgtg ttgattcgat
    32281 gtgtcgcact ttcttcatac caagaaagtc gttgtgccaa taatgctttt atttcttctc
    32341 ttgtttttcc aagagctaaa ggtcgaatag acgtttcatc ttgttggata cgatcaacta
    32401 gacagtccgc ttctgcctct aagtaaagaa caatcggctg tgtttttaaa aaacgccgat
    32461 tttctgcact ttggacaatc cctccaccag tcgcgataat tttgtcttca tttgacagct
    32521 ttcttaaaat atctgactcc acttttcgaa agcttttttc accgtaatag tgaaaatatt
    32581 cggcaatcga caattggata tgttcctcaa tctttgtatc taaatctaca tactcttttt
    32641 gtaaacattg agctaaccct ttaccgattg ttgtttttcc agcgcccatg aaaccaatca
    32701 gcaaaatgct agccatgagc ttctcctcct gacacaagtt ctgctaaatc gtcaaaaaat
    32761 gccggataag agattgaaac agcttcagct ccttctagtt cgcaaggtcc atcagctatc
    32821 aaagcagcta cttgcagcat cataccgatt cgatgatcgc ctctgctgga cacttttgcg
    32881 ccatgcagag gtgtcggccc atggatgatc agaccgtctg ctgtcgcttt tatctttgct
    32941 cccattttct gtaattcttc tgccaccgca tcaattctgt tggtttcttt tacttttaat
    33001 tcttctgcgt ctttgatgat cgtgattcct tctgcttgag tagccactaa agctagaatg
    33061 ggcaattcat caatcagacg cggaatgatt tctccaccaa tcgttgcttt cttcaaatga
    33121 ctcgtcttga cacttaaatc agccgattgg ttctgttcat tgtgattcgt ctgggtaatc
    33181 gctgcgccca tctcctccaa cacatctaag ataccagttc ttgttggatt gaccccgaca
    33241 ttttttaaca acagttggct ttccggaaca agaagtcctg ctgctagaaa gaaagcagct
    33301 gatgaaatat cacctggaac agttatctgt tgaccagtta atttttgcgg tccggtcact
    33361 atgatcgttt tatcttccac tgtgatcctt ccgccaaatt gacgaatcat ttcttccgta
    33421 tggttacggg aacgttcttt ttcaacgatt acggatgtgc cttctgcttg taatgccgca
    33481 aacagcaaag cagatttgac ttgcgcactg gctacaggca tattgtatcg gataggtgat
    33541 aaagagcggg ttcctgtcac agtgattggt agatactggt cattttcttc tccttgcaag
    33601 tcagcgccca tctcgcgtaa aggttccatc actcgcccca ttggacgttt agacaacgaa
    33661 gcatccccga acagagtagt cgtaaaaggc tgtccagcca aaatacccat caataaacgt
    33721 atggtcgtac cggagtttcc catgtcaatt gattcggttg gttggacaaa tgagtggcta
    33781 ccgcgaccat gaatcttgat cacctcttcc tcttcttcga tctcgacacc taattgttgg
    33841 aaggcatgta aagtactcag acaatcttct gcgcgcaaaa aattttgtat agtcgtcgtt
    33901 ccttcagcaa ttgctccaaa catgatgctg cgatgggaaa ttgatttatc tgctggtatc
    33961 ctaacagtcc ctcttaatcc atgtatttgt tgtaataatt gcatttatcc agcctcctat
    34021 ttgatccagc aatgataact ggtattagac ataatgattc tttttgcttg taacaaatcc
    34081 tgttggtttt taaaactgat ttgcagtatt cctaaaatat cttccctggt ctcttggatc
    34141 ttcaaattaa tcaaagaaac tcccgctttt ccgatcagtc cagttacttc tgcaataacg
    34201 ccaggttcat ctggcacatc gataaataaa tcaaaaaagg caggaatcgc gccttgctta
    34261 tggacaggca actggtctcg actttctttt gcccgattga aaaaagaaaa gatttccgat
    34321 tggttctcag tcatgatcca ttctgctatt tgtgtcattt cttgttgcca atctgccaat
    34381 cgttccaata atatctgctt attgctcaac aaaatatctg tccacatgac aggatctgag
    34441 gaagcgatcc tagtgatatc tcgaaagcct cctgctgcca gttgtttggc tcgcggatac
    34501 tcttgattga attgttctgc ctgattgact aagccagctg ccagaatatg tggaaaatga
    34561 cttagcattc ctgttatacg gtcatgttct cttgctgtaa gagttacata ttttgctttg
    34621 gttccgcaaa atagttcttg cagtttttca acatcttttt tcgtcttttc caaaggtgta
    34681 aagatgtaat aggcattttc aaatagttta gcatctgctg cctgtatacc tgatttatga
    34741 gaaccagcca ttggatgtcc accgacaaac cgaagatttt tctgctcagc ggatgtcacg
    34801 acttgctgct tcgtactgct gacatccgta atgatgacgt gatcagacaa gttgattctt
    34861 tcaaggacat caagataagc taaggttgta cggacgggaa cggctaataa gataacgtct
    34921 gcttgttctg cacctgtttc aaaatcactt gcaacgtaat caacgatctg ctgttctact
    34981 gcttgctcac gggtttttga ctgactatcc caaccaacta ctatcagttc tggattggtc
    35041 tgacgaatac aaagtgccat agaagcacca atcagtccaa gccccacgat aaaaacatgt
    35101 tgtgccatct tatttgcccc ctttttagta atgtttcaag tagtcacggt actccttcac
    35161 tgcagctttc aactcaacaa gcgaatcact tgggaatttt gacaatagct ctgtcgctac
    35221 cacagtggct acaacatttt ccgcaacgac gctagctgca ggaactgctg tactatccga
    35281 acgttctaca cttgctttgt aaggttcttt tgtatcgata tcaacacttt gaagtggctt
    35341 gtacaatgta ggaatgggtt tcatgacccc gcgaatgaca agtggttcgc cattggtcat
    35401 tccgccttcg aagccgccta gattattggt acgtcggaaa tagcctcttt cttcttccca
    35461 taggatctca tccatcactt ctgagcctgg taatgaacca gaagtgaatc cgaccccaaa
    35521 ttctacgcct ttaaaggcat tgatactaac cacagcttgt gcgattttgg catctagctt
    35581 cgtgtcccat tgtacgtagc ttcctaatcc agctggaacg cctccaacga gcacttctac
    35641 aattcctccg atcgtatctc catttttctt tgtctgatcg atcaaatctt ttattctttg
    35701 ctcaactgct ggatcaacaa ctcgtacttc tgacgcttca gaaatttctt tgatttcttg
    35761 aatcgagata tttccaggta tttctgcttg gattccacca agacaaatca cgtgtcctgc
    35821 tacgatgatg tcaagttctt ccagaatttt cttggcaatt gcgccgattg ctacacgcat
    35881 ggtcgtctct cttgcagagg accgttcaag cacattccga agatctcgat gatggtattt
    35941 cataccgcct accaaatccg cgtgtcctgg acgcggacgt gcaactcgcc gcagtgattg
    36001 gtctttttca ggcacttctg ctacagacat gacagttgtc caatttttcc aatctttatt
    36061 ttctaccact aaagtgacag gtgaaccaat tgttttgccg tgacggatgc cagacgtgat
    36121 ccgaacacga tctttttcga tcttcatcct tcctcctctg ccgtaaccag cttgtctgcg
    36181 agcgagttcg tgattgattt cttcttctga cagaggtaat cctgctggta gtccttcaat
    36241 aatggctgtt aattctggtc catgggattc tcccgcagtc atataacgca tcttacagtc
    36301 ctcctaagta atctttgaat gtctctgtgg gaacaggaac gatttttgct tgcccaatct
    36361 tttctagcaa gatgatattc aattggcttc ctcttgcttt cttatcatgg gttatcgcgt
    36421 ggaataattc atctggatga cttagatcgt acgtaatcgg cagatggaat ttttgaagta
    36481 tcctttctaa agcagctgtc gatccagttg gagacaagcc gattttttct gcatgagaag
    36541 tgatggctac cattccaata gctactcctt ctccatgact gactacccca tatcctgctg
    36601 tattttcaat tgcatggcca attgtatgac caaaatttaa aataagtcga tttccttggt
    36661 caaagacatc ttcttccact acttttcgtt tgatttccaa cgtagcagta atgattggaa
    36721 cagcatgttc cagaagatct ttttcgtctt tcagctcgtt cagctgtttc caaagcgttg
    36781 catctgctat agctgctgat ttgatgattt cagcgattcc ttctcgtaaa cgacgtaccg
    36841 gcaaagtttt tagaacctca ggatcaatca atacaccagc tggctggcta aatgttccga
    36901 caaggttttt cgctttgttt gtattgactg ctgtcttccc gccaatacta gaatctactt
    36961 gtgcaagtag cgtagtcgga atttgaacga aatggatccc acgcatgaac gtcgaggcca
    37021 cgaatgctgc tagatcaccc accattcctc caccaagtgc aagtatccca tcgctgcggg
    37081 taaaatcatt atctgctaaa aaatcgtata actcagctgc ttgttctaaa gatttactgg
    37141 tctcacccgc cggtacgatt gaaaggactg gttcaaaatt agcttgtctc aattggaaca
    37201 tgattttttc tgcatatagt ggagcaacgt ttgtatcagt gatcacagct actttttgag
    37261 gtctccaaat cgtttgaagc catgatccag cctgttctag tgcgcctttt tcaatatgaa
    37321 tatcgtattt ttggttatca acatgtacag taagcattcg tttcacttct ttcttttaaa
    37381 aagctggaat taatcgagtg tctgaatctt ttccattgct tctttcatga tcgcaacttc
    37441 tttcatcatg cgtgagtaag tcttttcatt caacgattgg ggaccgtctg accaagcatt
    37501 tgcaggatca ggatggatct ctacgatcat gccatctgct ccgcttgcca cacccgcacg
    37561 agccattggt ggtacaagtt cccaaattcc tgtaccatga cttggatcaa caatcacagg
    37621 gaaatgagtt aattttttca tcaatgggac agcacttaaa tcaaacgtat tacgtgtcgc
    37681 tgtttcgtaa gtccgaatcc cacgttcgat aaaaatgaca ggagatttgt cttcgactgc
    37741 aatgtattct gcagcattaa gccattcatt gatcgtaccg gaaattccac gtttcaatcc
    37801 aacaggttta cctgttttac cgacagcaga tagcagttta aaattttgca tattacgtgc
    37861 gccaatctgt aggatatcgc tgtactcagc caccatatcg atatgccctt catccatcac
    37921 ttcagtaatc actttcattc caaattcatc agccgcttga cggatgtatt tcaatccttc
    37981 ttcttctaaa ccttggaaag catatggaga agtacgtggt ttgaaggcac cacctcgcag
    38041 aatttttgcg cccccggctt tggccattcg cgcacattct ctgatttggt ctaatccttc
    38101 gatagaacaa ggaccagcca tcgtcacgaa actgccgtca ccaattttta ctccgtctac
    38161 atccacgact gtattgccag gatggaattc tcgagaagtc aatttgtatg tgtttaaaat
    38221 acgcacagca ttttctactc cttcatagcg tgtaaatgct ccttcttgta aacttcgcgt
    38281 atcacctttt agtccaatca ctactcgttc tgttccttga tcgattgaga ctttgaatcc
    38341 ttctttttcg atatgatcga cgactttttt tatttgttct tccgttgctt cttttttcat
    38401 aattactatc attgtttttc ccgctttctt ttgtttactt atctatcttg gctgattttc
    38461 tctacttttt caaggtggtt ttacattaat tcctactaaa tgaccgtgtc ctgttgttct
    38521 cattttcgct ccttctttcc tacttatgac ccagataatc aacaaaaaaa gatgtccctg
    38581 taggaaattg cctctacagt gaacatcttc aacgagcctt aaaaagcgct gcaaaaatat
    38641 aaaatagcca ctatagatga atttctctac gtggctgtaa gtcgtgttta aaaatcttta
    38701 gccatcatag atacaaacct ggtttgtttg tacccacgaa gtcatgaata caaacctatc
    38761 taaaatagct aaagtaatac gtattcagtt ttgtaagttt tctagatttc ataaaaaatc
    38821 gctccaaact gatgtaagaa ttgtacctag tatagacata aaaaaatcga ctgtcaatca
    38881 tttttatgaa acttttttta attttctgat agttaacttt gtttttcaga aaaataacaa
    38941 ttggttttat tgaagaaaat agtcatgaac aaaagtatct gagaactttt ttactctctt
    39001 gttcatgaca tatagaataa agcagctttg tcttgtaaac tccgtttttc aaataataat
    39061 ctaaagattg ctactgaata ataaagccga tttcatggtc ctcttcgctg ctgattcggt
    39121 aaggtgactc aacatccgct ccccagctat tgataccccc gactcctctt acttttgcaa
    39181 aaatcgtaag aaccgttcgt ctttctggcg gtagttcttc tatatgtgtt gcctgttcca
    39241 gttcttctgc tttgaatggt aaagcggaaa aagcaaatgg cgtatcctgc tgttcaaatc
    39301 gcaacgaaaa atcttcttga tctttatccg tattatccaa cgtttgattt cttgttagtt
    39361 ctacccattt cgtttccata tgcatgccgc attcttgggg aacaaggtat ggggaaacgg
    39421 ggagtcctgc cactttataa ataccttcga caccaccagc catccgatcc ggatatgttt
    39481 cccctgatag gccttcatac tcaaaaaatt ttgctttcgt tggcataatg aaccttaaac
    39541 ccaatgcggg taattccggt aatccttttt gtccataata atgcatattg acaagcattt
    39601 taccctctcc agcgatttgg taggaaattg tcacttgggt ttttggtact gtgttgattt
    39661 ccagtgtaaa aatgatttca accgtttcag cgtattcatt acttgaatat tgattattta
    39721 ctggagcaat cggcaaatcg atctttttat tgtccaccag tacttctatt tctttgactt
    39781 ttggatacaa tccggctcct aaccacatac cggatttaaa tccaaatccg ctacctctgt
    39841 cattatcggt cgttgctcgc caaaaaactg gaagtgtttc ccggtatagc cattcttttc
    39901 cgtttttatt gagcgattcc agtccgcctc ttgtataaga aaaaatataa tgcgtattcc
    39961 ccgatttgac acctaatgta cagtcgccga agacgattgc aactttttcc gtatccatat
    40021 gacttttatt tactgtattt ttcataataa tatcttacct cctgcatggc gggtttttct
    40081 tgtccatctg caaaaacgat cccattccct gagaattcat agtcacttgg tttatcgtca
    40141 aagtcaccgc catagcgcat cacttctctg cctgtgattg gatcagtcac ttggatcgcc
    40201 tgatctttaa aatcccatat atagcctcct tggtacattt cataacgatc aagcaacgtc
    40261 atgtaagaat ccattccacc taacgaattc cccatgtcat gcatatattc acataagatg
    40321 aacggttttt ccgctttatt ttcaaggtag tcaatgatct cttctggtgg cgcatacatg
    40381 cagcttttta catctgaaat cgaatcttcg aatgctggat ttggaaaatt cccttcataa
    40441 tggacaagtc gatcggggtc tacttttttg aagtaatcgt tcatttgtcg aatgttttct
    40501 tccgcataag attcatttcc aagagaccaa aaaagaatac ttggatggtt ttttaatgtt
    40561 tcgaaattcg tttttgctcg atcaagaaca gccgcctgcc actgaggtac tgcacctggt
    40621 acgttgtaag aaggctcaaa tgcgcccatt ttttgccatg agccatggga ttcgaggttc
    40681 gtttccgcca tcacatggat tcctgcttgg tcgcataaat aataccaagt gatctggttg
    40741 gggtaatgag atgtccgtac gccgttgatc tttgcatttt ttatgatagc caaatctttt
    40801 tccatatctt ctatagtgat ggcgcgtcct tttttcggat gccactcatg tcgatttacc
    40861 ccgttgataa tcatccgttt tccattgaag tagatgattt tatctttgat ttcgatttta
    40921 cgaaaaccga aggaatagtc aatatactcc gttactcttt tttcttcgtc tatcgtctcc
    40981 aattcaagct tatataaatt gggctgaaaa tgcccccaca gtgccacatt ttcaaatcca
    41041 tcgatgcgaa tcgtcatttg ctcttttaac ggatattgct gttgatagat ttccttccca
    41101 tcaggatcta gtatcgctac attcagctga ctgtttttgg ctacttgctc aaaagacaaa
    41161 tctacttcaa gtttgccatt ttgacatttg tcatctaagc gaggtttgat ccaaagatct
    41221 tctaaatggt tttgtcctcg agcaactaag aagacatctc tgaagatccc aaaaaatcgg
    41281 aaaaagtcct gatcttccaa gaatgcagct gtacttcttt tgtaaacctc cacagctaga
    41341 atattttctt cttccaaata aggtgttaaa tcaaattcag acggagtgaa cgaatcttcg
    41401 gcatacccga taaaatgacc attcaaccaa ataaacattg cttgttctac accagcaaaa
    41461 tgaatcgaga tttgttttcc ctttagacct tcttctaatc gaaaagtcgt caaatacgaa
    41521 ccaaccggat tgtctttagc agctgaaaag atcccttccg cctctttctc agggcttgtc
    41581 gtgaaagggg gacggcgaaa tacatgtccc tcccacggat acatcgtatt gatgtaatgg
    41641 atcttgtcat aatttgctaa ttcaatatga ctaggaacag tgatttcatc aaaagaacag
    41701 gcatcaaaat cttttttata aaatgttttt ggtcgtaccg aaggatttgg cgaataacaa
    41761 aaacgccatg atccatttaa agactgttta agggaggatg tggtttctac ttcttccctt
    41821 gttgcataac aaacatgatc gctatgtgca tttgattggt tcactctaaa aacttgtgga
    41881 tcatctaacc attttatatt tgctttcata cttttttcct tctctctcat aactttttga
    41941 aatgaagcag ttctgtttgg aaatcacctt gaaaatcggg gaagtaaagt ccaactgtca
    42001 tcaattcggc ccccgaaaat atccgcttcg aggtctgttc ctgataaagt gcctcatcat
    42061 ctaaccccat cagatatact tcatggaacg ctggttgagc tgaagctagt atcctcccta
    42121 aaaacacaat agcttcatct tgtttaggag acacaaacat ccatgcggca atattttctt
    42181 caaaaggact cgccaaacga taataatggc catactggat caccggacga atcgtttgat
    42241 aggatataat ttggtctttt acttgctttt tttcttcagg agacagcttt gtcaaatcca
    42301 gttcataacc aaaaacagcg ctcatagctg tttctcctct tgttttcaac gaagtagatc
    42361 tgtcggtctg atgatttggt acagctgata catgagctgt gatggacgaa atcggataag
    42421 caaggcttgt tccatattgg attttcatac gctccactgc atccgtgtta tcacttgtcc
    42481 atgactgcgg catataataa ataaacccgg catcaaaccg tccacctcca ccagaacagc
    42541 cttcccataa gatttccgga taatcagttg tcaaatgttc aagtagttca taaagcccga
    42601 gcatataacg atgcgctact tctccttgtc tctgtgggtc aagggtaatc gaatagacat
    42661 ctgaaagcga acggttcata tcccatttga tgtagtcaag aggaatcgta tctaaaatct
    42721 ttctcatttg tttctcaatc gtctgccgaa catctaacct tgtaaaatcc agtacatgtt
    42781 gtgatctgga agcagaaggc attctacctg gctcctgcat aagaaaatca ggatgtgttc
    42841 gatacagttc agaatcaacc gagatcattt caggttcaaa ccataaacca aatttcaatc
    42901 ctttgctatg agcataatct gctatttcac gtaatccatt tgtcaacttg ccttcatact
    42961 cgaaccagtc tcctaatgaa gaattgtccg catctctttt tccaaaccag ccatcatcta
    43021 atacgaacat ttcgatgcct agctcctttg cttcatcaat gatcgccttg attttttcac
    43081 tattgaagtc aaagtaagtg gcttcccagt tattgaccag aatcggccgt tcagcaaatt
    43141 gatgacgacc tcttgcgatt ctttcacgta gcaactcatg gtggatctga ctcattttgt
    43201 tcaatccctg acttgagtaa gaaaggatca tttcaggtgt ttgaaaagac tggcctgcat
    43261 tcaattccca cgaaaaacga tggctgttga tcccaccgac gactctcaat tgattgatct
    43321 gatctttttc aagttcgaaa gaatggttgc cggaataaac aaattgaagt ccgatcgctt
    43381 ctccagtatt ttctgtcgta tgatgatgaa ctaaagcaat aaagttattc atatggtgac
    43441 tgctcgttcc tcgacggctt tcaaatactt ttgttccaga taaaacagat tgccgtgaaa
    43501 tctgtctttc attgacatgt gctcctggca aagcaatgac ttcatcaaat cgcctagtat
    43561 gggcaaagtc tagctggaaa ctagccgctt tttctagttt gattgtttct ccggtttcat
    43621 ttcttatctt tacagatcgt gtgatcactg ctctatcagt aaatatggta tagaacaaat
    43681 cgaaataaat cgctgtttct ctatccttaa gtgaaatcac caacgtttgt gcctctgact
    43741 tgtccatcac ataagtttga ggcaagcctt cgatctctgg cttcccatct gtaatataat
    43801 ggctgtcata tctcagatcc agtaagtctg atccattctt gcgctgaatg atcgatgcgg
    43861 gcgttcgaaa gtccatgctg ccatgagaag agtattcttg cggtagtgta tctttcgaca
    43921 gagaccggtc aggatttaag ggaacatttc ctgaaaatcc acgatctctt cggggataat
    43981 ttttattgtc tttgtactgc ttgactggtt tgccaaaata aatatgcgcc aatacattgc
    44041 tttcttctat ctttatcaga taagaaagaa atgtatttcg taaatggaag actcggctct
    44101 tttcatcaaa taagatgaaa tttttatctg tttttctatg cgtaatatcc atcatttcac
    44161 tcccaacaaa acggaaaccg tttacttttt tttataattc ctgtatactg tttactaaac
    44221 aattttgtca ataaaaattt tttatttttt tgaggagtgt gtaaaaatgg ttggaattag
    44281 agatgtcgca cgaaaggcaa atgtctctcc tgctacagtt tcccgtgtgt tgaataacga
    44341 tcagacgatc catgtggctc agactactcg gcaacggata gtagaggcag ctgctgcgtt
    44401 ggattatgac atcagtactc gtaaatatat caaaaagaga atgccttcga ttggtgtgat
    44461 ctctacgatc ggcaaagcga gcgaagaaca ggatatctat tacaaacatt tgcgagtagg
    44521 gatggaagaa gaagctcgac gtctgcattt agggatgaat cgaatctata atctatctga
    44581 taatcctaaa caatgggaag atctagatca gttaggtgcg gtgatcgttg ttgggactgt
    44641 tacgaaagaa tcgattaccg ccttgttaaa acaaaacatg caaatagtcg tcgtggataa
    44701 ccctgacatc cagcaagagg tcgatatggt gtatgcagat cttgaacgga tgactaaaac
    44761 cgtactggaa ttgttcttag aaaatggtca taaaagaata gcctatatcg ggggatataa
    44821 agtcgatatg gatgaattcg gtgggaagca gatttctata aatgaaaaga ggatgcgtgc
    44881 ctatcagcat tttatgatcg atcatcactt agaaaaatat atgtcttatc atctaggtgg
    44941 ctggacagaa aaacaaggtg aggtacttgc gactaatcta ttagaaaatg aatcccctct
    45001 tcctactgcc attcttgtcg ggagcgatcc aatggcaatt ggtgtctatc gggcattgaa
    45061 acaaaaaaat atccaaattg gagaagagat tgttgtagca agctttgata atatcgagac
    45121 aatcgcaaac ctctcccctg gcttgaccag tgttcaaatc aatgcgaaat caattgggca
    45181 agcagctgtt cgaatggcat tggagcgaat tgacggtata cgggacgaac caatcatttt
    45241 gacttatcct gcacgattga ttgttaggga aagttttatc ccaagaacaa aaaggtattg
    45301 aaagagaggt tgtgaaaagc cgcttcgttt cttcatactc aaaacgaaac ggctttttca
    45361 caacctctta cttatactta ttcacaaaat ccaatgtctg ctggtaacaa ttttgcgatt
    45421 cttttagcgt gtaatcaaat tggtattcat gggtaatctc tttttttgta tctgtataaa
    45481 acaaggattg cacgggtacc tttaattctg tcaaccgatt ttctaatgct tgaccttgct
    45541 cctggaatga gtatgcattt ccgtcagtta tatacgttgg cggaaattcc ttagaaacat
    45601 ggtcagccac actagcttct ttcaattgct cactcgtttt ccaatctttt gttccaatca
    45661 acgaccacgc aacggttttg acgaaaaact tcataaatcg atcatccgaa tgctgctgtg
    45721 ctgttagctt caaatcaaca ggtccgcaat aagaaatggt tcctttcaga ctatcttttg
    45781 gaagtagctg tttgattttc atttgtttag catacgcatc attcgtttgt atagctgcat
    45841 attgcaatgc gatttgtgaa cctgcactat ctccaccaaa aaataattgt tccatgtcta
    45901 gcaatttgtc gtctttttct tcagctaata aatctttgat cagttcattc acttgaagaa
    45961 cttgattagg gtattctgaa tcaggcgcta attcataatt catcgcaacc actgcgatgt
    46021 tggcgtctga aactaatttt gtcgcaaatt ctttgacacc cgatttatct ccgccgacga
    46081 atcctccacc atgtacccaa aatagaacag gaacaggccc cttgctgttt tttggatagt
    46141 agatgtcata agtattctct ttatacttgg actcatacgt caaatctgtt tttttcgcca
    46201 cgtttttttc tgcttgatcg aacgtttttt tatcagtgat ttgaacagta tttgaaaaca
    46261 tatggttgat caagaatgct ccaggtctgg gggaaagctg gaaggcaaga aagattgcaa
    46321 acgcgatccc aagaatgatt ccgaagaacc atccgataaa tttaagaatt ttctttttca
    46381 taaggctccc tcagtagtta gtattaagca ttctgattat accatagtgt tatttcctat
    46441 caagctctcc tagatgattt aatgattttc cccaaacttc ttctgcaatg cttttgacaa
    46501 aagaaatttt ttccattgtt cttcaaacaa aacattttct tcttctgttg aagcaaatcc
    46561 acattgagga cttaagcaca aacgatccaa aggcagatat cgtgtcgctt cgtagatccg
    46621 gtcaatcact tgctggtgct cttccatttc gccatttttc gttacagtca aaatctaaga
    46681 caactaattt atcttcagag actttctcta aaagaacaaa actcccagca cgttcactat
    46741 cgtattctaa ataataggca cggacatttt cttgagtaaa ataatgggtc tgctacgtcg
    46801 tcatcccccc tgatgccgcc aaaatcgaat aataatttcc gcgtcaaaca tgtatattta
    46861 gtgttgaatc atttgagaat atgaaaataa aaatcttttt gatcaatcag ttaatcaaat
    46921 tgaaataacg aaaaatctga gacagaatga cgttcattct gacccagatt tgattttgca
    46981 caactcaaca gtccttattg gcaaatcgcc aaaataatgt agactaacta aaaagactaa
    47041 gaaacaataa caaaagttaa cacttcttag tctttcttta gcacgtctat ttattaaaga
    47101 ttttagggcc ttttctcatg tttttacgat cattcttagg tgtttccatt tgtttggatc
    47161 ggcctccacc ttgtttcttt ttgatcaatt ctttcatttt ttctttttgg tccattttta
    47221 ttctccttta acgaatgaag cgactaattc gatatgatgg gtttgcggga aaagatctac
    47281 aggctgaact ttcttcacgg cataacctgc ttctgcaaat aacttagcat ctcttgcaaa
    47341 agttgccgga ttacatgaga tatagacgat tcgttctggt gacatttcga ttgctgattc
    47401 aataaaatga tgatctaatc cttttcgtgg agggtcaact acgatcacag atggtttgat
    47461 ccctttttct tgccattttt tcataacttt ttctgctttt ccgacttcat aatgaacatt
    47521 ttcgatttta ttatttaaag cattgaactg tgcatcttca attgcttctg gaataacttc
    47581 catcccatac accttatcca ctctgtctgc taaagacaag ccaatcgtac ctattcctga
    47641 ataagcgtcg atcacaatgt cttttttatc taactcggca aactcgatgg ctgtttggta
    47701 cagatactct gcttgttgag tatttacttg ataaaatgac tttgctgaga ttcgataggt
    47761 ttttcctaat agctgatctt cgatatagtc tctgccatat aaaacttttt ctttgtctcc
    47821 taaaatcaca tttgtttttt cttcattgat attttggatg accgaaacca cttctggcaa
    47881 ttcttcatga atagcttcag caatttcctt ggatttaaag aatttttctt tacgagtaac
    47941 taaaacgacc atcatttcat gggaataatg accacgacgg ataatgatgt gacgaagctg
    48001 tccttcattt ttttcttcat tatatcctcg tacttggaac cgttgcaaga tatctctgac
    48061 tactttgatt gcttgatcga tgacaggatc ttggatataa aaatcttcga tagggactag
    48121 atcatggcta ttctttttgt aaaaaccggt ggctaactgt ccatcgatac ggcgaacagg
    48181 gatctgtgct ttattacggt agccgaatgg ttctgccatt ccgaccgttg gaagtacagg
    48241 aacttctggc atcttagcaa tcttcgccat tacatgctct acttgttttt gtttgaagac
    48301 aagctgttgt tcatacttca aatgggctaa aggtgcgata cctatgcgaa gtaatgcatt
    48361 gttttctaca ggctgacgat caggtgattg tgtaataaat gtttcgactt ttccatatcc
    48421 aaacttattg cctgctttta ctactagtat ttctacttct tctccaggga gtgcattttc
    48481 gataaataat ggataaccat cgatttttgc tacccccatt ccttcataac tcaagtcgac
    48541 gatttcgact gtatatcgtt cgtttttctt tactatttgc tgttctgtca tattttgctc
    48601 ctttaagcct atttttcaat cctactgaac tctactataa atcagctaat tacacaacta
    48661 aaaccggcac tgaccttact acgttcagca ccggcttttg attaattctt ttgattaggt
    48721 gtttcgtctg ctatttttcc gtcaccgtcg atatcttcat ccgttaaacg ttcgacttct
    48781 tttacgaatt ctttgctgac aacctctaat tcttcttcat ctgtacctgt gattgcttca
    48841 tcaggaatct gatcggtatt ggcaaagaac tcaatgtgtt gatgaagatt ttggaaatgc
    48901 atcggtgctt ccccgccgta ttcaccatct aagttgatca ttactggtcg attgatatca
    48961 tcttctgttt caacgaacaa actgcttgtt ttggtataga ttattcttgg atcatcgaca
    49021 tgtcgaccgc cattcagcat gagtgctgca atatgcaaga tctcaaagat atttgctgtc
    49081 ttcacaatga ttaaagaaaa cttcccatca tccaatttgg catctggcgc gatctgttcg
    49141 aatcctccaa cagagttcgt cagccctagg aaaaacatcg aagcatttcc gatatattcc
    49201 ccttcatcat acttcaagcg cattttgatt ggctttactc gagggagcat ctctgctcct
    49261 ttcgcaagat aagcaagata accaaaaata ctctttagtt ctgatggaac ttcataagtc
    49321 aattctgtca aatagccacc tgcagcgata ttgatgaagt aactgttatc agaaaggccg
    49381 atgtccattt ttacggtttg gtgttttttt atcacttctg ctgctgcttt aatattatct
    49441 ctaggaattt ttaatgctcg agcatagtcg ttcgttgtac cagctgggat gatggccatt
    49501 ttaggacggt gttcaagcgg agcgattcca ttaaccactt cgttgattgt gccatcgcca
    49561 ccagctgcca ctactagatc aaaacctagt tctgcgactc tacatgcttc attcttggct
    49621 gaatctggtt ctggtgtagt tgcataggcg ctggcttcaa atcctgcttc ttctaatgcc
    49681 tgtaggatat ccgccaaatt ttttttcaat aattcttttc ccgaggtggg gttataaatt
    49741 acgcgtgctt ttttcatttt cagcctctat cgtttcgcta attcatcttt aagtagctta
    49801 ttcacgacac caggatttgc ttgtccttta gttgctttca tgatttgacc gactaggaag
    49861 ccaaccgcac gatcttttcc atttttgaaa tcatcgacag attgttggtt gttatccaaa
    49921 atttcattaa tgattggcag tagtttgttc ggatcagata attgcaccca accatttttt
    49981 tcaactactt cttgggcttc tccgccattt gtgatcaatt cacggaagac ttttttggcg
    50041 atttttgagc tgatcgttcc atcggcgatc aaacgaatca ttcctgctaa gttctcaggt
    50101 gtcaattttg tctcagctaa ttccagtttc tcactgttca agtaagcaga aacttcaccc
    50161 atcaaccagt tagatgcttg cttggcatct gcaccatttt ccaaaacagc ttcaaaaaaa
    50221 tcagacattt cttttgtaag tgtcaatacc attgcgtcat actctggaag tccaaactca
    50281 cggatatagc gttcgcgacg agaagctggc atctcaggca aactagcttt tactttttga
    50341 atccattcgt catcgatcac gaatttcggg atatcaggct ctgggaagta acgataatca
    50401 ctagaccctt ctttgacacg catcaaaact gttttattcg ttgtttcgtc aaaacggcgt
    50461 gtttcttgtt ggatttctcc tcctgaaaga agaacttttg cttgacgttt ttcttcaaaa
    50521 gctaaacctt ttttcacgaa agataaagag ttcaagtttt tcaattctgc tttcgtaccg
    50581 aactcttctt gtccataagg gcgaagagaa atattggcat cacagcgcat agagccttct
    50641 tccattttca catcgctgac ttctgtaaat tggataattg atcgcaatgc ttctagataa
    50701 gcgtatgctt cttctggtga acgcatatca gcttcagaaa cgatttcgat caaaggcgtg
    50761 ccttgtcggt tcaaatcaac gtaagaataa ccgccaatac catgcatgtt ttttccagca
    50821 tcttcttcta aatggacacg ttcgatccgg atttttttct ttttaccttc tacttcgatt
    50881 tcgatccatc cgtcatgacc gattggttga tcaaattggg aaatttgata tgcttttgga
    50941 ttatctgggt aaaagtaatt tttccggtca aaatgagtat ctttagagat ttcacagttt
    51001 aatgccaaag ctgctttcat cccgaattcc agcgcagctt tattcattac tggcagcacg
    51061 cctggataac cccagtcgat cacgtttgta ttgctgtttg gttctgcacc aaaatgagct
    51121 ggcgctggag agaaaatttt tgagttggtt tttaactcga cgtggacttc tagtccaatg
    51181 actgtttcaa aattcattcg tctttccctc ctaaaatagc tggttgttgt ttatggaatt
    51241 ctgttgcttg ttcaaacgca tatgccgctt gatacatcgt tttttcatca aaataattac
    51301 cgatgatctg caatccaact ggtaatcctt ctgataaacc tgccggtaca gacatacctg
    51361 gaagtccagc caagttgaca gggatcgtta agatgtcact catgtacatt gtgattggat
    51421 cgttaatgtt ctcaccgatt ccgaatgcaa ctgttggtga agatggcccg atgatcaagt
    51481 cataatctgc aaagactttt tcaaaatctc gtttgatcaa tgtacggact tgtcctgctt
    51541 ttttgaagta agcgtcataa taacccgcgc ttaaggagaa tgtacctaac atgatacggc
    51601 gttttacttc ttcaccgaat ccttctgaac gtgagttgac atatacatca tctaatgatt
    51661 tcacattttc agagcgataa ccgtagcgga taccatcgaa tcgttgcaaa ttggaacttg
    51721 cttctgatga ggcaatgata taataaacag ccacaccgta ttttgaatga ggcaagctga
    51781 cttcttccac aacagcacct aatgaacgga atgtttctgc tgctttcaat acagcttctt
    51841 ttacgccagg ggccacacct tcgcctaagt attctttagg taaagcaatc ttcattcctt
    51901 tgatgtcgcc tgtcagtccc tctgcaaaat caggaactga aacgcctgaa gaagtaccgt
    51961 ctttttcgtc ataaccgctg atagctgtca atgctaatgc attatctttc accgtacggg
    52021 tcattggtcc gatttggtca agagatgaag caaaggcaat caaaccaaaa cgtgagacac
    52081 ggccataagt aggtttcatc cctacgatgc cgttaaaagc tgctggttgg cggatacttc
    52141 ccccagtatc actacctaaa gaaactggaa tttgtccagc tgcaactgct gctgcagaac
    52201 caccagaaga gccaccaggt acttttgttg ggtcccaagc attttttgtt ttcttgaaat
    52261 aagaagtttc tgtacttcca cccatagcaa attcatccat gtttagctta ccaacaggga
    52321 tcatatctgc ttgatagatt ttatccatga ccgttgcatc ataaattggt tcaaaattgt
    52381 aaaggatctt agatgcagcg gttgtcaaga tatcttttgt tacgatatta tccttgatac
    52441 caattggaat accagcaagg acgttgcttt catcaatccc tttttcatct aaagcttttg
    52501 cctgttccaa tgctttttct tcgttcaatg taatgaatgc ctcgacctca ggctctgttt
    52561 ctttgattcg ttcaaatgtt gtttttgtca aatcttgtac agtgatttct ttcgatacaa
    52621 gcatatgatg cagttcttct agagaacgat tgtgtaattc tgtcattatg cgccagcctc
    52681 cccattatcg atgattgcgg gtactttgat aaagccgtct tctgtttccg gcacattttt
    52741 caataattca tcgcggttcc atccagcttg cgcccgatct tgtcgcatca cgtttactgt
    52801 ttcaaccaca ttcgacgtaa atggaacacc ttccgtatct acttcttcca gcatttcaac
    52861 catatcgatg attttaccta attggtcggt aaaacttgca agttcttcat ctgcgaacgc
    52921 aagttttgct aatttggcaa cgtgtttcac ttgttcttca ctaatcgcca tactacccct
    52981 tctttctaaa cgagttaaaa actttgctct ctattttaac acaagagtgc cgtgtctgaa
    53041 attgtttatt gaaatttgtc tgaaaaaact catttttcat tcttctactg agcgactttt
    53101 agccaatgca tctgccattt gatcttctgt ccaaacttcg attcctaatt cttgtgcttt
    53161 ggtcagcttg ctgccagcat cttctcctgc taccacgata tccgtttttt tcgatacgct
    53221 accagtcact ttcccgccaa gattttcgat cgtctctttt gcttcttcac gagtaaatcg
    53281 tgttagttta ccagtaagga ctactgtttt ttctttaaaa ggagactcga cttcttgcaa
    53341 ttgggcattt cgaagccctt tgtattcgaa attgacgcct gcttgtttta attcattcat
    53401 caattcatgg acttcttcgt tactgaaata agtgactaca ctgtcggcaa tgatatcacc
    53461 gatcgtatca agcgcaataa tcgactcata gtcactttta ctcaatgttt ctagatcacc
    53521 gaaatgttct gctaaaattt ttgctgcttt cgctccgaca tgtcggattc ctaacccgaa
    53581 aatcaaccgc tcgaccgaat tatctttgct gttgtcgatt gctgtcaaaa tattatttgc
    53641 tgatttttct tttattttgt ccaacgtcaa taactcttct tctgtcaatt tgtaaagatc
    53701 agcaacatct gctactagtt ttttatcata catctgttct agtacgcgcg gccccaaccc
    53761 atcgatattc atcgcatttc ttgaaacaaa gtggttcaaa ccttctttca tttgagcagg
    53821 gcatttagga ttgatgcacc gtaaggctac ttcttcatcc aaatggacaa gttcacttcc
    53881 acaaactgga caatgtgtag gcaattggta ttcttcgctg tctttaggtc gtttatctaa
    53941 cacgacttgc gaaacttctg ggatgatgtc tcccgcttta taaatcagaa ctgtatcttt
    54001 caagcggata tccttcaact taatataatc accgttatgc aggctggcac ggcttactgt
    54061 cgttcctgct actcggactg gtgtcatgat agctgttggc gttactaccc ctgttcgacc
    54121 gactgtccat tcgatgtttt cgattagcgt ttcaacttct tctggcggaa acttgtaagc
    54181 tgctgcccat ctcggtgctt tgatagtaaa accaagttga tcttgtagcg agaattcatt
    54241 cactttgatg acgattccat cgatttcata gggcaaatcc acacgtttgt catgatattc
    54301 ttcaatgtat gaccacactt catcaatcga atgacataga cgtttttccc gattcgtgtg
    54361 gaatccaatt ttttctagct cttctagcgc atcatactgg gtttttgctt gcataggacc
    54421 gaaatcagct actgtgtata aaaaagtgtc cagatttctt ttcgctgtga ttttagaatc
    54481 taactgacgc aaagaaccag ctgcggcgtt ccgaggattc gcaaagatat cttttccctc
    54541 tgcttctctg tcttggttca attggacaaa tgaccgtttt ggcatgaaac attcgcctct
    54601 tacttcgatg ttcattggtt cttttaattt gattgggacc gaccggacag tttttaggtt
    54661 ttcagtgatg ttttctccta cagttccatc cccgcgagtc gcacctctca cgaaatttcc
    54721 atcttcataa cgcaaagaaa cggaaagtcc atctattttt agttcacagc aatagtcaac
    54781 tacacgtccc actgcttttt gtacacgttg atcaaaagct atcagctctt ctttgctgaa
    54841 aacgtcgttc aaactgtaaa gcggaatatc atgagtgact ttttcaaaac cttctaatac
    54901 ttttccgcct actcgttgag tgggagaatc agacgtgatc aaatcaggat attgttcctc
    54961 gattgctact aattctgcgt attctttatc ataaacatag tcttccactg ttggtttatc
    55021 ttctacgtag tattcgcgag accattggtt cagtcttgta cgaagttctt ctgctcgttt
    55081 agcagcttcg tcaatcgtca tttcatgttc catcatttac cctccactac taagcttttt
    55141 caattggcgc aaaggcagcc aaaagacgtt ttactccttg acttgggaaa gccacatcta
    55201 gttctagatc cttcgacgtc ccgcttactc ggacgactgt tcctagaccc cattttttgt
    55261 gtttgacttt atctccaggt ttccagctgt tattttcccc tccagtagca cttttagagg
    55321 gaacaaccgc tgtctctggc tgtttatagc tatatgctgt ttttttagcg aatacaggcg
    55381 tttttttagg cgtaggacgc atgccttcga tttccagtaa ctcctgatcg atttcttcta
    55441 caaaacgaga aggtcggtta tattgcgtcc gtccatataa tgttctggag aaagcatttg
    55501 ttaaatacaa cgcttcttct gctcgcgtga tccccacata tgcgagacgg cgttcttcct
    55561 ccagttcgct ttcttccatc aatgctcgag ataaaggaaa aaccccctct tctagaccaa
    55621 tcaggaatac aactggaaat tctaatcctt tggctgcatg caaagtcatc aatgtcactt
    55681 gggaagcatc ttcttctaaa ttgtcaatgt cggaaacaag tgccagatca tttaagaaga
    55741 ccgttagttt ttcttctggt gcatcagcgt cttcttcatt ttgctgttcg aattgtttgt
    55801 cgaattcttg tgtgaccgtt aaaaattcct ccaagttttc gatgcgtgct tgtgcttcta
    55861 atgtattttg gatttttaag tcttctagat atccgctacg atccaaaacc tctttcgtca
    55921 attctgtcac tgtcaagtat gggatcatct gagtgacttc ttggatcatc tctccaaatg
    55981 cacctaattg ctgtcctgct tttccaccga tatttgccag atccacattt tgcgctgctt
    56041 caagcagtgg ccattcatga agagaagcaa aactgcgtag tttttcgata gatcctggtc
    56101 cgatgcctcg tttgggcgaa ttaacgatac gctcaaaact gattgaatct tgaggattta
    56161 ccagcacatt caaatacgct aaaatatctt tgatttcttt acgatcgtag aacttgtgcc
    56221 ctccaaccat tttataagga atatttgctt tcaacagagt ttcttccatt acacgagatt
    56281 gtgcgttcgt ccggtacaag atggcaaagt cgccgtaatt ccgatgattg cttcgaatct
    56341 cttcttgcat ccggtctacg ataaaacggg tttcgtctcg ttcattatct gcacggtagt
    56401 aagtgatctt gtttccttct ttgttttctg tccacaagtt ttttggtttt cgattgctat
    56461 tgttttcaat gacttggttt gctgcgctaa ggatgttttt cgtggagcga tagttttgtt
    56521 ctagcaaaat tactgcagca tctggataat ctttttcaaa atccaagatg ttctgcatat
    56581 ctgcaccgcg ccagccgtaa atactttgat ctgcatctcc cacgacgcac aggttcctaa
    56641 atctacctgc caataaattg actagcgtat actgtgcgtg atttgtatct tggtactcat
    56701 ctacatgaat ataatggaat ttgttttgat aatacgtgag tgaatcagga tgttcttcaa
    56761 ataatcgaat cgtattcatg atcaggtcgt caaagtccat acattgatta tattgaagct
    56821 ctttttggta agcagcataa cattttgccg caatctcttc gtaataactg ccttgcattt
    56881 ttgcaaaatc ctgaggggtc tgcaggctgt tttttgcttg agagattgtt cctaatatac
    56941 ttcgaggatc atattttttc ggatcaatgt tcagttcttt tagtatccgt ttcatcaagg
    57001 tcagctgttc agaagaatcg atgattgtaa agttccggtt atacccgata aaatcaacat
    57061 ctcttcttaa aatacgcaca caaatcgagt gaaaagtcga tacccagaca tcttcgcctc
    57121 cagatgcgag aatcgcattg acacgttctt tcatttcttt tgcagcctta ttggtaaaag
    57181 taatcgctag gatgttccaa ggattgactt ctttttcctc aatcaaatag gcaatgcggt
    57241 gtgtcagcac tctcgtcttt ccacttcctg cacctgccat tagcagtaat ggtccgtcag
    57301 tgtgaagaac agcttctttc tgacgggggt tcatcccgtt taataattca gctttggatg
    57361 acattaacaa catcctttct tttatcaatc ttgttaatta tagcattttc ttggctttca
    57421 ttaaactagc tagttcaaaa tcatttgttt ctttttatgg atttttcctt gtcaattcgt
    57481 tgatttcgcg ctaaattaaa agaaagcgag gcgtttgaat ggaaaaaaaa ggaaaaatca
    57541 agaaattaga taagactcag atatatttta ttctaaaagg aatatttgtt ggactgctga
    57601 caggaatcgt cgttagcctg tttcgattat ctatcgaaaa gctaagcgat ttagttcgaa
    57661 gtatctatga aatgagtcgg gaacagccga tttacttaat tggcctagct atttgctgca
    57721 tccttgcagc atttttcgtg ggttatctgg tcaaaaatga accggatatc aagggaagcg
    57781 ggattccaca agtagaggga cagcttcgcg gggaattatc aatgaactgg ttctctgttt
    57841 tatggaaaaa gttcattggc ggtgtacttt cagttggtgc gggccttttt ctaggtagag
    57901 aaggaccttc tatacagctt ggcgcatcca ttggacaagg agctggacaa ttgtttcgga
    57961 gtccatcttc agaagaaaag attttaattt ctagcggtgc aagtagcgga cttgcagcag
    58021 cattcaatgc tcctatcgca ggattactct ttgttttaga agaaatccat cactcctttt
    58081 ctccacttgt ttggctgaca tcatttgctt cagcaatcac tgctaacttc gtctctcttt
    58141 actttttcgg cttgagacct gttttatatc taggtgacct tccttctttg ccgttgaaat
    58201 attatggttc cttagtgatc ttaggagtac ttctaggcat cttaggtttt atttatcaga
    58261 atgtcttgct ggctttgcca aaattttaca accgattgcc tttaccagct tattttcacg
    58321 ggttcgttcc tttcttgctg attttgccaa ttggttattt atggcctagt actctaggcg
    58381 gaggcaatca aatcgtttta gcttttggac atgcttcctt gccaatcatt atgattatcg
    58441 gtttgtttgt tcttcgattc gttttttcca tgatttctta cggtgggggt cttccagggg
    58501 ggattttcct tccgatattg acattggggg ctttaatcgg tagcttttat ggaaacatta
    58561 tggttgattt cgggatggat ccaatctaca tcaaggattt tgtcgttatt gccatggctg
    58621 gttatttcac agcgattgga aaagcaccgc tgacagcaat tattttagtg acagaaatgg
    58681 ttggctctct taatcatctg atgcctttag gattggctgc tcttgttgct tatatcgtca
    58741 atgaccttct cggcggtaat ccaatttacg aatcattatt ggaacgtcta cttggaaata
    58801 ctcagaccgc ctcactaaaa ggtgcaagga caacttttga ttttccagta acggcagaga
    58861 gttctttaga cggattaatg gtacgagact tcaattggcc taaaaatatg ctgctaatct
    58921 ctatacgcag aggaaatcag gaaatcttga ctcacggtga tacgatcatg aatgtaggag
    58981 atattttggt tatcttgact gatgaagcaa acttgccaag aatcaagcag gaaatcgagg
    59041 ctaaatcttc tatcacaaat ttgaagcaat cattttaacg aacatggaaa aactatttta
    59101 caacaactat tcatctacac tacttgattc tatatgattc tataaaaaaa gaagaggctg
    59161 ggaaaaccca gcctcttctt ttttatttct atgcttctgc tactgcagga gcttttctca
    59221 ataaaccata aatcacacca gatacgactg ctccgatagc aataaacacc aaatacatga
    59281 ccgggtgact cagtaagaat acaacgaaaa tcccgccgtg aggagccagt aatttgattc
    59341 cgaaagctcc gactaatccg ccagttaaag cagaaccaat aataaaactt ggaatggcac
    59401 gaatcgggtc agctgctgca aatggaatcg atccttccgt gacaaacgat agtcccatca
    59461 cgatgttcgt caaaccagca tcttgatctg ttttgctgaa tttattctta aacaaacgtg
    59521 tagcaacgaa aattgccaat ggaggtacca ttcctccagc cataacagaa gccatcacga
    59581 cacttccgcc agttgcaaca gtcgtagcta aagttgctgt accaaacaca taagcggctt
    59641 tgttgattgg accgcctaaa tctgccgcca tcattccagc taataatgca ccaagtaaag
    59701 cagcattcga accactcata cttgtcaaga aaccgttcaa tgcatcattg attgctttca
    59761 tagggacatt gaccagcaac atcaagaaac cagtaatcag taagccaaac actggataaa
    59821 agaggatcgt tttaattcca tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa
    59881 atacgatgac ataaccagct aagaatccac caatcagtgc acctaagaat cctgcaccgc
    59941 ctgtattagc taatgcacca gcagcaaatc caacgatcaa accagggcgg tcaccgatac
    60001 ttgaagcaat aaatcctgct aataccggca gcatgaaacc aaatgcagct tgtccgattt
    60061 ggttgaacca gctagcagct tgattgtaat tccctagatt tgataattga tcatgtggca
    60121 cgccaataaa ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca ccaataacga
    60181 aaggtaacat gtgtgatacg ccgttcatca aatctttgta aattctttgt ccgatagtac
    60241 catccgcatt tccttcttct gaactgcttt cttttccatc accatggaat actggtgcag
    60301 taccactaag ggctagattg atcaactctt ctggtttacg gataccatca ctgacaggac
    60361 gattgatcag ttcttttccg tcaaagcgat tcatctctac ttttttgtct gctgcaacga
    60421 ttacaccttc tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt ccttctgaac
    60481 cgttcgtttc gactttgatc tccacaccca tttctttcgc ttttttcttc aaagcatctt
    60541 ctgccatgta agtgtgagcg atccctgttg gacaagctgt aacggcaaca acgaattttc
    60601 tttctggatg agcagaagct tctgcagctt gtgtttcttg ttctgctttt tcttctgctt
    60661 ctttttgctc ttcagctgtt tggaacaagt cttggacttc ttctggtgta tttgcttctt
    60721 ttaattttcc tacgaaatct gcatcaatca gcaatcgaga tagtgctgct aatgcttgca
    60781 aatgtgtgtc gtttgcgcct tctggagctg cgatcatgaa aaacagataa gttggctgtc
    60841 cgtctaaggc ttcataatct actccttttt ggctttttgc gaataaaact gttgcttctt
    60901 taactgcact gtttttcgcg tgaggcatgg cgattccatc accaagacct gtagaagttt
    60961 gtgcttcgcg ggctagaatt ccttctttat atgtttcgat atcggaaatc cggccggcat
    61021 caaacatttt ttggaccatt tcatcgattg cgcctttttt atcagtcgct tgcaaatcca
    61081 tgatcatcgc ttctttgacc attaaatcct tgatattcat gattatcctc cttgaaacta
    61141 atcaattttt gtgatttcaa cttctggcag caactcatca atcaattcgc gagctgccaa
    61201 gtcatcagaa aaagctgttg cgcttccgca agcaactccc catttaaagg cttcgattgg
    61261 atctttcgta cggctgtaag ccccaataaa gccagcaatc attgaatccc ctgcacctac
    61321 tgaatttttc aatgggcgaa ctaatacatt cgttttataa gttccttgtt ccgtgaagag
    61381 caaagcaccg tctccagcca ttgagatcag cacattctgt gccccttcag ctagtaattt
    61441 tttcccatac ggaatgattt cttctacaga ttggaaggtt gtgttgtaaa gctcagctaa
    61501 ttcgtgattg tttggtttga tcaacaacgg tttcttaaca agtgcatttt ttaggtcatc
    61561 acctgttgta tcaatcacga aatctgcacc tttttctcga atgatatcaa tcagttcttg
    61621 ataaaatcct tttcgcaggc tagctggcgc gcttcctgac aatacgacga tatctccttg
    61681 tcctactgtt cctaatgctt gcttcaactc atcgatttct tcatctgaaa tagatgggcc
    61741 taagccattg atctcagttt cagtgtctga ttttagtttg atattgattc ttgtatcttc
    61801 ttttacggtt gtgaacaaag actggatttg ttctttttca agccacttcg aaataaattc
    61861 gccagtgaac cctcctaaaa accctagtgc tgtagattga gaatcgatcc gtttcaaaat
    61921 acgggaaaca ttgatccctt ttcctccagg taacttgaag tcttccttca ttcggttcaa
    61981 atcgcctagt tttaacccat ctacgcgtac gatataatca attgatggat taagtgttac
    62041 tgtgtaaatc attgtattgc ctccttaatt gttgtctttt cacggaattc attaacgaat
    62101 tctaatggac aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag
    62161 gacaactggt tgaatttggt atgatcaatc aatacaaatg tctcttcgga atttttcatg
    62221 gctaaacgtt taacagctgc ctcttctgga tccggagtcg tgaatccttg ttcaagatgg
    62281 gcaccgttca ttcccataaa tgctttatta aaatgaaaat tttttagttg ttctatcgca
    62341 ttagatccta aaacagcatt agttgccagc tttatcgttc cacctaaaat aatggtagca
    62401 agctccaaat caactaaatt tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt
    62461 tttccttgca gaaaaggaat catttctaac gtagtagagc cagcgtctaa ataaatgaca
    62521 tcttcgtcat taatggtttc agctgccgat ttggcgattt gctgtttttc gtgaacgttt
    62581 ttgagtgatt tttcactcat attctgctca aaattcaata attgctcttt cttagctccg
    62641 ccatgaactc tttctagaaa cccactattc tccagctctt gtaaatcacg tcttatcgtt
    62701 gattcagaag cattcaaaag gtttacaaga tcttgtgatt tcacgatttt ctgctgttca
    62761 agcaagtcta ggattttttg tctgcgttcc tctgtaagca ttttcacgat cacctcaata
    62821 caaataatag catatttttt ttctactagc aatataaatc agtcaaaaac gttcataaaa
    62881 attataaatc agtcaaaaaa aaaataaaaa accagtcaga aagactggtt ttttattttt
    62941 ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt tttcatataa
    63001 acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat cccgccaaac
    63061 atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa gagacagacg
    63121 gataataatg atgccggaac cattattcga taatagagtt gtttatccat tttcttcacc
    63181 tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa attgcacacg
    63241 cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt tctttattcg
    63301 tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt ctgacaggga
    63361 ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt catctgttac
    63421 ctctacttct agatattccc taatataata acaataagct tcgattccat tatccaaagc
    63481 aacagtcgga tcgataactt tatagcacat catgcgtgat cgattctcgt caacccaagg
    63541 aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat ctccttgttg
    63601 gatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacgg gttgcatttg
    63661 tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt caacgaatgg
    63721 cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa gctggtccct
    63781 aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt ctcgataaag
    63841 ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt ggatgtcgag
    63901 aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt cccaacacga
    63961 agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc aatcgactac
    64021 tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct ttttctcaaa
    64081 caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat aagctcgcat
    64141 ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa tcaaaaattg
    64201 cttttctttg aaaacagaga tagacaatac attttttatc aataattgat tatcttccaa
    64261 gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt ctcttaccga
    64321 tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt gttgtgtgaa
    64381 agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat cgcttatttc
    64441 ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata atttatcttc
    64501 gccaatcatt ttctcacctc tttcaaatct ttttataatt taattataac caatgataaa
    64561 gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat agactaagta
    64621 aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga taatttagat
    64681 aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa agaagagatc
    64741 gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa acaaaagtaa
    64801 gttaagctca aaaaa
//
LOCUS       DACEKG010000003        63305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEKG010000003 DACEKG010000000
VERSION     DACEKG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.003"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..970
                     /locus_tag="IW664_000126"
     CDS             107..970
                     /locus_tag="IW664_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0847524.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IW664_000127"
     CDS             complement(1099..2721)
                     /locus_tag="IW664_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0847525.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IW664_000128"
     CDS             2970..3491
                     /locus_tag="IW664_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847526.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW664_000129"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW664_000129"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0847527.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IW664_000130"
     CDS             5572..6390
                     /locus_tag="IW664_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0847528.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IW664_000131"
     CDS             complement(6493..6690)
                     /locus_tag="IW664_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847529.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IW664_000132"
     CDS             6859..7437
                     /locus_tag="IW664_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0847530.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IW664_000133"
     CDS             complement(8482..8985)
                     /locus_tag="IW664_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0847531.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9343..10368
                     /locus_tag="IW664_000134"
     CDS             9343..10368
                     /locus_tag="IW664_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0847532.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLVGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10691..11569
                     /locus_tag="IW664_000135"
     CDS             10691..11569
                     /locus_tag="IW664_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847533.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12045..12161
                     /locus_tag="IW664_000136"
     CDS             12045..12161
                     /locus_tag="IW664_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0847534.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13089..13427
                     /locus_tag="IW664_000137"
     CDS             13089..13427
                     /locus_tag="IW664_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847535.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13521..13751
                     /locus_tag="IW664_000138"
     CDS             13521..13751
                     /locus_tag="IW664_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847536.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14151..14522
                     /locus_tag="IW664_000139"
     CDS             14151..14522
                     /locus_tag="IW664_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0847537.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14796..15488)
                     /locus_tag="IW664_000140"
     CDS             complement(14796..15488)
                     /locus_tag="IW664_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0847538.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15491..16276)
                     /locus_tag="IW664_000141"
     CDS             complement(15491..16276)
                     /locus_tag="IW664_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0847539.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16513..17913
                     /gene="lacG"
                     /locus_tag="IW664_000142"
     CDS             16513..17913
                     /gene="lacG"
                     /locus_tag="IW664_000142"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0847540.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17938..18255
                     /locus_tag="IW664_000143"
     CDS             17938..18255
                     /locus_tag="IW664_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0847541.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18268..19942
                     /locus_tag="IW664_000144"
                     /pseudo
     CDS             18268..19942
                     /locus_tag="IW664_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            19956..21587
                     /locus_tag="IW664_000145"
     CDS             19956..21587
                     /locus_tag="IW664_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0847542.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22078..23643)
                     /gene="guaA"
                     /locus_tag="IW664_000146"
     CDS             complement(22078..23643)
                     /gene="guaA"
                     /locus_tag="IW664_000146"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0847543.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23702..24625)
                     /locus_tag="IW664_000147"
     CDS             complement(23702..24625)
                     /locus_tag="IW664_000147"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0847544.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24821..25426
                     /locus_tag="IW664_000148"
     CDS             24821..25426
                     /locus_tag="IW664_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0847545.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25734..26750
                     /locus_tag="IW664_000149"
     CDS             25734..26750
                     /locus_tag="IW664_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0847546.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26891..28192
                     /locus_tag="IW664_000150"
     CDS             26891..28192
                     /locus_tag="IW664_000150"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0847547.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28238..28900
                     /gene="deoC"
                     /locus_tag="IW664_000151"
     CDS             28238..28900
                     /gene="deoC"
                     /locus_tag="IW664_000151"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0847548.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28921..29319
                     /locus_tag="IW664_000152"
     CDS             28921..29319
                     /locus_tag="IW664_000152"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0847549.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29385..30467
                     /locus_tag="IW664_000153"
     CDS             29385..30467
                     /locus_tag="IW664_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0847550.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30679..32241
                     /locus_tag="IW664_000154"
     CDS             30679..32241
                     /locus_tag="IW664_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847551.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32234..33367
                     /locus_tag="IW664_000155"
     CDS             32234..33367
                     /locus_tag="IW664_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0847552.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33367..34323
                     /locus_tag="IW664_000156"
     CDS             33367..34323
                     /locus_tag="IW664_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0847553.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34422..35588
                     /gene="deoB"
                     /locus_tag="IW664_000157"
     CDS             34422..35588
                     /gene="deoB"
                     /locus_tag="IW664_000157"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0847554.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35612..36430
                     /locus_tag="IW664_000158"
     CDS             35612..36430
                     /locus_tag="IW664_000158"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0847555.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36447..37154
                     /gene="deoD"
                     /locus_tag="IW664_000159"
     CDS             36447..37154
                     /gene="deoD"
                     /locus_tag="IW664_000159"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0847556.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37452..38138)
                     /gene="gpmA"
                     /locus_tag="IW664_000160"
     CDS             complement(37452..38138)
                     /gene="gpmA"
                     /locus_tag="IW664_000160"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0847557.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38310..38891)
                     /locus_tag="IW664_000161"
     CDS             complement(38310..38891)
                     /locus_tag="IW664_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0847558.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39024..39707)
                     /gene="rpiA"
                     /locus_tag="IW664_000162"
     CDS             complement(39024..39707)
                     /gene="rpiA"
                     /locus_tag="IW664_000162"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0847559.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40026..40439
                     /gene="rpsL"
                     /locus_tag="IW664_000163"
     CDS             40026..40439
                     /gene="rpsL"
                     /locus_tag="IW664_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0847560.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40523..40993
                     /gene="rpsG"
                     /locus_tag="IW664_000164"
     CDS             40523..40993
                     /gene="rpsG"
                     /locus_tag="IW664_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0847561.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41087..43174
                     /gene="fusA"
                     /locus_tag="IW664_000165"
     CDS             41087..43174
                     /gene="fusA"
                     /locus_tag="IW664_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0847562.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43346..44533
                     /gene="tuf"
                     /locus_tag="IW664_000166"
     CDS             43346..44533
                     /gene="tuf"
                     /locus_tag="IW664_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0847563.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            44940..45248
                     /gene="rpsJ"
                     /locus_tag="IW664_000167"
     CDS             44940..45248
                     /gene="rpsJ"
                     /locus_tag="IW664_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0847564.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45279..45908
                     /gene="rplC"
                     /locus_tag="IW664_000168"
     CDS             45279..45908
                     /gene="rplC"
                     /locus_tag="IW664_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0847565.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            45936..46559
                     /gene="rplD"
                     /locus_tag="IW664_000169"
     CDS             45936..46559
                     /gene="rplD"
                     /locus_tag="IW664_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0847566.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46559..46849
                     /gene="rplW"
                     /locus_tag="IW664_000170"
     CDS             46559..46849
                     /gene="rplW"
                     /locus_tag="IW664_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0847567.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            46888..47721
                     /gene="rplB"
                     /locus_tag="IW664_000171"
     CDS             46888..47721
                     /gene="rplB"
                     /locus_tag="IW664_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0847568.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            47764..48042
                     /gene="rpsS"
                     /locus_tag="IW664_000172"
     CDS             47764..48042
                     /gene="rpsS"
                     /locus_tag="IW664_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0847569.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48065..48412
                     /gene="rplV"
                     /locus_tag="IW664_000173"
     CDS             48065..48412
                     /gene="rplV"
                     /locus_tag="IW664_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0847570.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48426..49082
                     /gene="rpsC"
                     /locus_tag="IW664_000174"
     CDS             48426..49082
                     /gene="rpsC"
                     /locus_tag="IW664_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0847571.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49085..49519
                     /gene="rplP"
                     /locus_tag="IW664_000175"
     CDS             49085..49519
                     /gene="rplP"
                     /locus_tag="IW664_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0847572.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49509..49697
                     /gene="rpmC"
                     /locus_tag="IW664_000176"
     CDS             49509..49697
                     /gene="rpmC"
                     /locus_tag="IW664_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0847573.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            49722..49988
                     /gene="rpsQ"
                     /locus_tag="IW664_000177"
     CDS             49722..49988
                     /gene="rpsQ"
                     /locus_tag="IW664_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0847574.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50046..50414
                     /gene="rplN"
                     /locus_tag="IW664_000178"
     CDS             50046..50414
                     /gene="rplN"
                     /locus_tag="IW664_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0847575.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50451..50759
                     /gene="rplX"
                     /locus_tag="IW664_000179"
     CDS             50451..50759
                     /gene="rplX"
                     /locus_tag="IW664_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0847576.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            50787..51326
                     /gene="rplE"
                     /locus_tag="IW664_000180"
     CDS             50787..51326
                     /gene="rplE"
                     /locus_tag="IW664_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0847577.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51345..51530
                     /locus_tag="IW664_000181"
     CDS             51345..51530
                     /locus_tag="IW664_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0847578.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51566..51964
                     /gene="rpsH"
                     /locus_tag="IW664_000182"
     CDS             51566..51964
                     /gene="rpsH"
                     /locus_tag="IW664_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0847579.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            51996..52532
                     /gene="rplF"
                     /locus_tag="IW664_000183"
     CDS             51996..52532
                     /gene="rplF"
                     /locus_tag="IW664_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0847580.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            52693..53049
                     /gene="rplR"
                     /locus_tag="IW664_000184"
     CDS             52693..53049
                     /gene="rplR"
                     /locus_tag="IW664_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0847581.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53070..53570
                     /gene="rpsE"
                     /locus_tag="IW664_000185"
     CDS             53070..53570
                     /gene="rpsE"
                     /locus_tag="IW664_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0847582.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53585..53764
                     /gene="rpmD"
                     /locus_tag="IW664_000186"
     CDS             53585..53764
                     /gene="rpmD"
                     /locus_tag="IW664_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0847583.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            53808..54248
                     /gene="rplO"
                     /locus_tag="IW664_000187"
     CDS             53808..54248
                     /gene="rplO"
                     /locus_tag="IW664_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0847584.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54248..55543
                     /gene="secY"
                     /locus_tag="IW664_000188"
     CDS             54248..55543
                     /gene="secY"
                     /locus_tag="IW664_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0847585.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55605..56252
                     /locus_tag="IW664_000189"
     CDS             55605..56252
                     /locus_tag="IW664_000189"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0847586.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56443..56661
                     /gene="infA"
                     /locus_tag="IW664_000190"
     CDS             56443..56661
                     /gene="infA"
                     /locus_tag="IW664_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0847587.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            56694..56810
                     /gene="rpmJ"
                     /locus_tag="IW664_000191"
     CDS             56694..56810
                     /gene="rpmJ"
                     /locus_tag="IW664_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0847588.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            56828..57193
                     /gene="rpsM"
                     /locus_tag="IW664_000192"
     CDS             56828..57193
                     /gene="rpsM"
                     /locus_tag="IW664_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0847589.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57221..57610
                     /gene="rpsK"
                     /locus_tag="IW664_000193"
     CDS             57221..57610
                     /gene="rpsK"
                     /locus_tag="IW664_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0847590.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            57690..58628
                     /locus_tag="IW664_000194"
     CDS             57690..58628
                     /locus_tag="IW664_000194"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0847591.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            58656..59036
                     /gene="rplQ"
                     /locus_tag="IW664_000195"
     CDS             58656..59036
                     /gene="rplQ"
                     /locus_tag="IW664_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0847592.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59308..60147
                     /locus_tag="IW664_000196"
     CDS             59308..60147
                     /locus_tag="IW664_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0847593.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60123..60992
                     /locus_tag="IW664_000197"
     CDS             60123..60992
                     /locus_tag="IW664_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0847594.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            60982..61779
                     /locus_tag="IW664_000198"
     CDS             60982..61779
                     /locus_tag="IW664_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0847595.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            61982..62728
                     /gene="truA"
                     /locus_tag="IW664_000199"
     CDS             61982..62728
                     /gene="truA"
                     /locus_tag="IW664_000199"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0847596.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        20065 a        11302 c        13493 g        18445 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacact
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc tttttttgtt gggaatccgg tttctttaac
     9121 aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg
     9181 gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg
     9241 aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc
     9301 ttggttcagc tttttttcca attactagga aggagggata gattgttcaa gacttatttt
     9361 cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt
     9421 attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta
     9481 gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg
     9541 tttctggtcg gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt
     9601 ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga
     9661 ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg
     9721 agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc
     9781 gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca
     9841 attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct
     9901 gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat
     9961 aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg
    10021 atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca
    10081 ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat
    10141 ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg
    10201 atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat
    10261 cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg
    10321 gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat
    10381 atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa
    10441 agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa
    10501 aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct
    10561 gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa
    10621 gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt
    10681 catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa
    10741 tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat
    10801 tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga
    10861 tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt
    10921 tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc
    10981 attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca
    11041 tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta
    11101 cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa
    11161 agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga
    11221 tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga
    11281 actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc
    11341 caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg
    11401 attattatca gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga
    11461 tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt
    11521 gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag tttagtactt
    11581 cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa
    11641 cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    11701 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
    11761 gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac tagctataac
    11821 aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa cgtaataatg
    11881 aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt ttaacaagtt
    11941 gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt tcgatttgtg
    12001 ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa aaaacagata
    12061 tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa cgtgcgctga
    12121 agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga aacttaatta
    12181 aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg tgtaaggttt
    12241 ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta gaatctttct
    12301 tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa ccacaaagca
    12361 cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa cctcttattt
    12421 tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg aaacattttt
    12481 agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt catgaaatat
    12541 ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata agcagttacg
    12601 aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa aaaaatcagt
    12661 gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg ccgaaaaaat
    12721 atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa taataagttt
    12781 tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag taataaagta
    12841 tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa ttaatggtta
    12901 aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg tgatactaga
    12961 ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa tgaaaaagaa
    13021 gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt agccaagtaa
    13081 cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt cgtgctacag
    13141 aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct agtgagccaa
    13201 ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt atggtggtaa
    13261 ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt gaaaaggtga
    13321 gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc attttaacgg
    13381 attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca ctagtgaaat
    13441 actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa tcgatcatca
    13501 aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt tattacgtga
    13561 atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt cgctactcaa
    13621 ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa actgtttttt
    13681 ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat tatgttagta
    13741 tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt tattgttttt
    13801 tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta tccagagtat
    13861 tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa attcagaagt
    13921 tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata atatttttga
    13981 tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt tggtttttaa
    14041 tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa gttggttacc
    14101 ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata atgagagatt
    14161 acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat gaattatctg
    14221 ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac tatattgata
    14281 tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata aaagctttta
    14341 tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta gtaggaaaat
    14401 taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct ttgtctatgt
    14461 tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg gaaatctttt
    14521 aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat gatttttaag
    14581 attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga tagagatgaa
    14641 cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt tcactaagga
    14701 tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat
    14761 ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga tgtggcagac
    14821 aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt tagctgtgcc
    14881 tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt tccgatcccc
    14941 gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca atcagccgga
    15001 gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt cccagggaaa
    15061 gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc gaaaatatca
    15121 aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg aatctgttgg
    15181 tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc gttttcattt
    15241 tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc gatctcagag
    15301 gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg accatgaata
    15361 ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat catatgcagc
    15421 caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc aaatataatc
    15481 cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt tgcagatcga
    15541 catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta atgagcagat
    15601 cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt ttattatcaa
    15661 cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct ccttcttcgg
    15721 tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat gcttttgtaa
    15781 actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg gtatttttcc
    15841 ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga atatcaaaaa
    15901 ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc gccaatagtt
    15961 cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag gaggctgctg
    16021 tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg tgagaaagtt
    16081 cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc agcttgcccg
    16141 atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct aattgattga
    16201 tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata gtaagcaatc
    16261 tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat ataaacggac
    16321 aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg tttgttacaa
    16381 tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt gttgacaaat
    16441 gtttcggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt atagatttga
    16501 ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc cacagcagcc
    16561 tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt
    16621 ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca tcaatatgca
    16681 aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc cattgcttgg
    16741 agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc tttttatcat
    16801 cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt
    16861 gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac aatagaagca
    16921 tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt gtggagtacc
    16981 tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt cccaccagga
    17041 atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc
    17101 cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt agttcactca
    17161 ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc ctttttagac
    17221 gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta ttcaactgaa
    17281 accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa
    17341 gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg gatcaatcac
    17401 taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca taacggtacg
    17461 ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat ttataaagaa
    17521 ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata tgacttgttg
    17581 ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga gaacgggatg
    17641 gggtataaag atcagtttga agatggcatt attatggatc agccgagaat cgactatttg
    17701 agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt gaaggggtat
    17761 tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa aaggtatggc
    17821 ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc ttactggtac
    17881 aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga agaaaagatg
    17941 aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg cgacgctcgc
    18001 agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca tgttgattcg
    18061 gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac aaaaatttta
    18121 gcggaagaag catctgggaa agagatggag atgggattca tttttatcca tgggcaggat
    18181 catttaatga ccactttatt gctgagagat ctgattcaag actttattgt actgtacaga
    18241 cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg
    18301 aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg tgacggattt
    18361 ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc tgcggttcct
    18421 gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa agtctataat
    18481 tattcgatgg gtcgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga
    18541 ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc
    18601 ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga
    18661 atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt
    18721 atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac
    18781 gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt
    18841 aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt
    18901 gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc
    18961 gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc
    19021 ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc
    19081 ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc
    19141 aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt
    19201 tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc
    19261 acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt
    19321 ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc
    19381 ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt
    19441 tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc
    19501 atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga
    19561 aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga
    19621 tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt
    19681 agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg
    19741 tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca
    19801 agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt
    19861 tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt
    19921 tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta
    19981 aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag
    20041 catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat
    20101 ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata
    20161 atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa
    20221 gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa
    20281 atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga
    20341 aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt
    20401 acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg
    20461 ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag
    20521 aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc
    20581 gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag
    20641 gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg
    20701 gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg
    20761 cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa
    20821 cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat
    20881 atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg
    20941 cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa
    21001 atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa
    21061 acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg
    21121 cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg
    21181 gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta
    21241 cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc
    21301 aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat
    21361 tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt
    21421 atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa
    21481 tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag
    21541 gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt
    21601 tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa
    21661 cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac
    21721 gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc
    21781 gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa
    21841 agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag
    21901 ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta
    21961 acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt
    22021 ttagctattg aagaatgttg atttataggg attccccctc catattatgt tggctcttta
    22081 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
    22141 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
    22201 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
    22261 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
    22321 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
    22381 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
    22441 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
    22501 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
    22561 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
    22621 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
    22681 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
    22741 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat
    22801 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
    22861 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
    22921 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
    22981 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
    23041 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
    23101 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
    23161 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
    23221 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
    23281 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
    23341 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
    23401 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
    23461 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
    23521 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
    23581 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
    23641 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
    23701 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
    23761 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
    23821 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
    23881 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
    23941 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
    24001 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
    24061 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
    24121 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
    24181 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
    24241 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
    24301 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
    24361 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
    24421 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
    24481 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
    24541 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
    24601 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
    24661 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
    24721 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
    24781 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
    24841 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
    24901 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
    24961 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
    25021 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
    25081 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
    25141 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
    25201 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
    25261 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
    25321 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
    25381 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
    25441 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
    25501 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
    25561 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
    25621 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
    25681 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
    25741 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
    25801 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
    25861 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
    25921 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
    25981 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
    26041 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
    26101 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
    26161 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
    26221 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
    26281 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
    26341 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
    26401 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
    26461 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
    26521 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
    26581 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
    26641 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
    26701 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
    26761 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
    26821 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
    26881 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    26941 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    27001 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    27061 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    27121 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    27181 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    27241 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    27301 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    27361 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    27421 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    27481 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    27541 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    27601 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    27661 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    27721 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    27781 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    27841 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    27901 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    27961 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    28021 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    28081 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    28141 tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa
    28201 agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga
    28261 ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc
    28321 aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga
    28381 acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa
    28441 cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt
    28501 cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga
    28561 tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttgttgaaac
    28621 atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc
    28681 tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg
    28741 tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa
    28801 cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg
    28861 agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc
    28921 atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat
    28981 gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat
    29041 caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct
    29101 tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac
    29161 acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc
    29221 cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt
    29281 gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct
    29341 gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat
    29401 tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca
    29461 acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga
    29521 tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag
    29581 aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat
    29641 cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa
    29701 cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata
    29761 caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg
    29821 caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg
    29881 aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg
    29941 ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat
    30001 tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta
    30061 tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat
    30121 tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg
    30181 accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat
    30241 tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg
    30301 acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc
    30361 taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa
    30421 tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata
    30481 gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt
    30541 atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt
    30601 caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg
    30661 ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc
    30721 aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa
    30781 attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc
    30841 ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca
    30901 agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt
    30961 gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg
    31021 cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca
    31081 gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt
    31141 ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg
    31201 acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag
    31261 tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca
    31321 gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa
    31381 ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag
    31441 cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa
    31501 gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc
    31561 gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc
    31621 ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa
    31681 catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca
    31741 ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg
    31801 ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga
    31861 acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt
    31921 atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga
    31981 ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac
    32041 aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt
    32101 gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat
    32161 gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag
    32221 ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact
    32281 ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc
    32341 catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga
    32401 aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc
    32461 agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt
    32521 ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat
    32581 cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt
    32641 tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac
    32701 atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat
    32761 gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg
    32821 cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa
    32881 gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta
    32941 tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc
    33001 agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac
    33061 ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat
    33121 cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca
    33181 gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt
    33241 tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca
    33301 agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga
    33361 actataatgt cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca
    33421 acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac
    33481 gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg
    33541 atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta
    33601 ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt
    33661 atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca
    33721 tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca
    33781 ggattagcag atattccggt cattggacca attttcttta aaaatacatt cttacctgct
    33841 tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc
    33901 ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta
    33961 tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct
    34021 gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc
    34081 atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca
    34141 ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct
    34201 tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt
    34261 gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa
    34321 taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt
    34381 gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc
    34441 tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg
    34501 ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag
    34561 agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc
    34621 aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact
    34681 gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg
    34741 atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt
    34801 atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa
    34861 ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt
    34921 tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata
    34981 tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa
    35041 gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag
    35101 aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa
    35161 tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta
    35221 tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg
    35281 gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc
    35341 cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa
    35401 acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca
    35461 gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa
    35521 cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat
    35581 taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga
    35641 aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg
    35701 ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga
    35761 gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc
    35821 tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc
    35881 catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat
    35941 tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat
    36001 taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat
    36061 gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa
    36121 agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg
    36181 accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt
    36241 agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg
    36301 aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt
    36361 tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac
    36421 attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga
    36481 tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt
    36541 ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat
    36601 ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct
    36661 atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg
    36721 gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca
    36781 gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca
    36841 atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa
    36901 atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg
    36961 aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat
    37021 accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa
    37081 caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag
    37141 cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca
    37201 agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt
    37261 ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca
    37321 atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc
    37381 aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt
    37441 tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa
    37501 acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg
    37561 atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg
    37621 tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact
    37681 ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt
    37741 gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc
    37801 cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt
    37861 aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga
    37921 tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa
    37981 gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg
    38041 attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat
    38101 tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata
    38161 ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc
    38221 catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca
    38281 gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca
    38341 ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga agaaataaat
    38401 tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg
    38461 atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta
    38521 cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg gaaatagatt
    38581 tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct
    38641 ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg
    38701 caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca
    38761 gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca
    38821 gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca
    38881 ataattttca tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa
    38941 tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta
    39001 ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct
    39061 ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc
    39121 ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc
    39181 agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc gtctgtacgc
    39241 aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga gccataaggg
    39301 atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg
    39361 atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg
    39421 ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg
    39481 actacgggga cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt
    39541 gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca
    39601 atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc
    39661 tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc
    39721 tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga
    39781 taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat
    39841 gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac
    39901 gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat
    39961 cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac
    40021 aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat
    40081 ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa
    40141 actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga
    40201 actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt
    40261 atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc
    40321 gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg
    40381 gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat
    40441 tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat
    40501 atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt
    40561 accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga
    40621 cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc
    40681 tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct
    40741 tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc
    40801 tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga
    40861 acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca acacaggtgc
    40921 ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca
    40981 ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt
    41041 ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt
    41101 ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac
    41161 tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg aaactcatga
    41221 aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc
    41281 tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca
    41341 cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt
    41401 tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac
    41461 ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt
    41521 atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa tccaattacc
    41581 aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat
    41641 gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga
    41701 attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact
    41761 tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg
    41821 ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa
    41881 aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc
    41941 agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag
    42001 cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac
    42061 tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa
    42121 aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat
    42181 ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg
    42241 ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc
    42301 agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc
    42361 tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg
    42421 cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg
    42481 tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt
    42541 ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata
    42601 cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa
    42661 tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa
    42721 agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact
    42781 ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc
    42841 tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc
    42901 tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg taactgctcg
    42961 tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat
    43021 ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg
    43081 tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga
    43141 aatcattaag aaaaacggcg gaaaagctga ataatttctt aaaaccggat agtcctgcaa
    43201 agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga
    43261 tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat
    43321 tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca
    43381 cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat
    43441 cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg
    43501 tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac
    43561 agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat
    43621 gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc
    43681 aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt
    43741 tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat
    43801 ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc
    43861 tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt
    43921 aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat
    43981 gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt
    44041 tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac
    44101 ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc
    44161 tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca
    44221 agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt
    44281 gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt
    44341 ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt
    44401 tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa
    44461 cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga
    44521 aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc
    44581 tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca ggatcaggaa
    44641 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
    44701 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
    44761 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
    44821 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
    44881 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
    44941 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
    45001 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
    45061 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt
    45121 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
    45181 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
    45241 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
    45301 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
    45361 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
    45421 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
    45481 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
    45541 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
    45601 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
    45661 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
    45721 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
    45781 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
    45841 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
    45901 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
    45961 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
    46021 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
    46081 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
    46141 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
    46201 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
    46261 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
    46321 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
    46381 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
    46441 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
    46501 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
    46561 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
    46621 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
    46681 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
    46741 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
    46801 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
    46861 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
    46921 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
    46981 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
    47041 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
    47101 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
    47161 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
    47221 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
    47281 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
    47341 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
    47401 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
    47461 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
    47521 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
    47581 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
    47641 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
    47701 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
    47761 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
    47821 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
    47881 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
    47941 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
    48001 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
    48061 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
    48121 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
    48181 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
    48241 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
    48301 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
    48361 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
    48421 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
    48481 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
    48541 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
    48601 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
    48661 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
    48721 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
    48781 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
    48841 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
    48901 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
    48961 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
    49021 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
    49081 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
    49141 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
    49201 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
    49261 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
    49321 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
    49381 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
    49441 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
    49501 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
    49561 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
    49621 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
    49681 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
    49741 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
    49801 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
    49861 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
    49921 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
    49981 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
    50041 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
    50101 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
    50161 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
    50221 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
    50281 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
    50341 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
    50401 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
    50461 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
    50521 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
    50581 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
    50641 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
    50701 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
    50761 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
    50821 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
    50881 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
    50941 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
    51001 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
    51061 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
    51121 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
    51181 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
    51241 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
    51301 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
    51361 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
    51421 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
    51481 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
    51541 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
    51601 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
    51661 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
    51721 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
    51781 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
    51841 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
    51901 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
    51961 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
    52021 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
    52081 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
    52141 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
    52201 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
    52261 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
    52321 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
    52381 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
    52441 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
    52501 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
    52561 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
    52621 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
    52681 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
    52741 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
    52801 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
    52861 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
    52921 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
    52981 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
    53041 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
    53101 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
    53161 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg tttggtacag
    53221 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
    53281 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
    53341 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
    53401 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
    53461 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
    53521 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
    53581 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
    53641 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
    53701 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
    53761 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
    53821 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
    53881 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
    53941 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
    54001 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
    54061 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
    54121 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
    54181 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
    54241 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
    54301 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
    54361 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
    54421 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
    54481 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
    54541 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
    54601 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    54661 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    54721 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    54781 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    54841 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    54901 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    54961 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    55021 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    55081 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    55141 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    55201 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    55261 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    55321 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    55381 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    55441 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    55501 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    55561 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    55621 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    55681 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    55741 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    55801 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    55861 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    55921 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    55981 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    56041 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    56101 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    56161 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    56221 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    56281 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    56341 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    56401 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    56461 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    56521 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    56581 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    56641 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    56701 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    56761 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    56821 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    56881 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    56941 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    57001 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    57061 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    57121 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    57181 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    57241 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    57301 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    57361 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    57421 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    57481 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    57541 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    57601 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    57661 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    57721 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    57781 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    57841 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    57901 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    57961 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    58021 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    58081 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    58141 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    58201 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    58261 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    58321 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    58381 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    58441 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    58501 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    58561 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    58621 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    58681 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    58741 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    58801 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    58861 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    58921 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    58981 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    59041 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    59101 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    59161 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    59221 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    59281 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    59341 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    59401 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    59461 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    59521 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    59581 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    59641 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    59701 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    59761 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    59821 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    59881 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    59941 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    60001 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    60061 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    60121 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    60181 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    60241 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    60301 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    60361 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    60421 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    60481 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    60541 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    60601 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    60661 gtagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    60721 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    60781 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    60841 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    60901 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    60961 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    61021 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    61081 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    61141 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    61201 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    61261 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    61321 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    61381 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    61441 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    61501 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    61561 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    61621 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    61681 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    61741 gtattattaa ctgtgctact gatctttttg agaggataaa cgttcagtgg gccgctatct
    61801 attcttttct tgctgatctg tttgcaagaa aggatgcgag cagtgtatga caagaaatat
    61861 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    61921 aaaaatttta taaaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    61981 catggtacgt tacaaagcaa tcattgcata cgacggaaca aattttaatg gattccaaaa
    62041 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    62101 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgattgg
    62161 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    62221 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    62281 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    62341 acgcagtcct tttcgacgcc attatgcaag ttattttcct taccctcttg atttggaaaa
    62401 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    62461 aggaagctcg atagaggata aagtacgtac gatctacgag gctaagatgg aggtaaatga
    62521 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    62581 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    62641 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacatc ctgaaggact
    62701 ttatttgtat gaagtgaaat atgaataaga ggttgtgaca gaagtggtca gcttcaagaa
    62761 ataagaagga atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    62821 aaggagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag
    62881 taataagaac aaccaaacaa aaatagctcg tcatattttt cgtttggttg ggcttattac
    62941 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
    63001 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    63061 taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    63121 tctgagtcac aagtaacaat ttcaaaaaac agcttctcat ctttgaggaa tatcgtgact
    63181 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    63241 aaacgatgga acgttgtttt gattgccctt aatgaggaca gtcggaacag gtttgtcatt
    63301 tttat
//
LOCUS       DACEKG010000004        60247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEKG010000004 DACEKG010000000
VERSION     DACEKG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.004"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..1156)
                     /locus_tag="IW664_000200"
     CDS             complement(137..1156)
                     /locus_tag="IW664_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0847597.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1178..2185)
                     /locus_tag="IW664_000201"
     CDS             complement(1178..2185)
                     /locus_tag="IW664_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0847598.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2178..2843)
                     /gene="pgmB"
                     /locus_tag="IW664_000202"
     CDS             complement(2178..2843)
                     /gene="pgmB"
                     /locus_tag="IW664_000202"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0847599.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2836..5130)
                     /locus_tag="IW664_000203"
     CDS             complement(2836..5130)
                     /locus_tag="IW664_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0847600.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5390..7555
                     /locus_tag="IW664_000204"
     CDS             5390..7555
                     /locus_tag="IW664_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0847601.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7859..8692
                     /locus_tag="IW664_000205"
     CDS             7859..8692
                     /locus_tag="IW664_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0847602.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8794..10494)
                     /locus_tag="IW664_000206"
     CDS             complement(8794..10494)
                     /locus_tag="IW664_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847603.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            11001..12161
                     /locus_tag="IW664_000207"
     CDS             11001..12161
                     /locus_tag="IW664_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0847604.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12177..12620
                     /locus_tag="IW664_000208"
     CDS             12177..12620
                     /locus_tag="IW664_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0847605.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13103..13633)
                     /locus_tag="IW664_000209"
     CDS             complement(13103..13633)
                     /locus_tag="IW664_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0847606.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13738..14289
                     /locus_tag="IW664_000210"
     CDS             13738..14289
                     /locus_tag="IW664_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0847607.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14442..14915)
                     /locus_tag="IW664_000211"
     CDS             complement(14442..14915)
                     /locus_tag="IW664_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0847608.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15053..16525
                     /locus_tag="IW664_000212"
     CDS             15053..16525
                     /locus_tag="IW664_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0847609.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16522..17232
                     /locus_tag="IW664_000213"
     CDS             16522..17232
                     /locus_tag="IW664_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0847610.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17464..17748
                     /locus_tag="IW664_000214"
     CDS             17464..17748
                     /locus_tag="IW664_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0847611.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18029..18448)
                     /locus_tag="IW664_000215"
     CDS             complement(18029..18448)
                     /locus_tag="IW664_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0847612.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18450..19034)
                     /locus_tag="IW664_000216"
     CDS             complement(18450..19034)
                     /locus_tag="IW664_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0847613.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19081..20556)
                     /locus_tag="IW664_000217"
     CDS             complement(19081..20556)
                     /locus_tag="IW664_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0847614.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20737..21048
                     /locus_tag="IW664_000218"
     CDS             20737..21048
                     /locus_tag="IW664_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847615.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21163..23499)
                     /locus_tag="IW664_000219"
     CDS             complement(21163..23499)
                     /locus_tag="IW664_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0847616.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23641..24309
                     /locus_tag="IW664_000220"
     CDS             23641..24309
                     /locus_tag="IW664_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0847617.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24312..25355
                     /locus_tag="IW664_000221"
     CDS             24312..25355
                     /locus_tag="IW664_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0847618.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25592..26473)
                     /locus_tag="IW664_000222"
     CDS             complement(25592..26473)
                     /locus_tag="IW664_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0847619.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26632..27360
                     /locus_tag="IW664_000223"
     CDS             26632..27360
                     /locus_tag="IW664_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0847620.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27516..27833
                     /locus_tag="IW664_000224"
     CDS             27516..27833
                     /locus_tag="IW664_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0847621.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27826..28434
                     /locus_tag="IW664_000225"
     CDS             27826..28434
                     /locus_tag="IW664_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0847622.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28489..29280
                     /locus_tag="IW664_000226"
     CDS             28489..29280
                     /locus_tag="IW664_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0847623.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29418..29906
                     /locus_tag="IW664_000227"
     CDS             29418..29906
                     /locus_tag="IW664_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0847624.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            29957..30829
                     /locus_tag="IW664_000228"
     CDS             29957..30829
                     /locus_tag="IW664_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0847625.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31005..31367
                     /locus_tag="IW664_000229"
     CDS             31005..31367
                     /locus_tag="IW664_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0847626.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31376..32128
                     /locus_tag="IW664_000230"
     CDS             31376..32128
                     /locus_tag="IW664_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847627.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32142..33944
                     /locus_tag="IW664_000231"
     CDS             32142..33944
                     /locus_tag="IW664_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0847628.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34221..35262
                     /locus_tag="IW664_000232"
                     /pseudo
     CDS             34221..35262
                     /locus_tag="IW664_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(35314..37458)
                     /locus_tag="IW664_000233"
     CDS             complement(35314..37458)
                     /locus_tag="IW664_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0847629.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(37815..39188)
                     /locus_tag="IW664_000234"
     CDS             complement(37815..39188)
                     /locus_tag="IW664_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0847630.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(39188..39583)
                     /gene="rbsD"
                     /locus_tag="IW664_000235"
     CDS             complement(39188..39583)
                     /gene="rbsD"
                     /locus_tag="IW664_000235"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0847631.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(39611..40528)
                     /gene="rbsK"
                     /locus_tag="IW664_000236"
     CDS             complement(39611..40528)
                     /gene="rbsK"
                     /locus_tag="IW664_000236"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0847632.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(40636..41649)
                     /locus_tag="IW664_000237"
     CDS             complement(40636..41649)
                     /locus_tag="IW664_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0847633.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(41730..42329)
                     /locus_tag="IW664_000238"
     CDS             complement(41730..42329)
                     /locus_tag="IW664_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0847634.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            42515..43216
                     /locus_tag="IW664_000239"
     CDS             42515..43216
                     /locus_tag="IW664_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847635.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            43226..45937
                     /locus_tag="IW664_000240"
     CDS             43226..45937
                     /locus_tag="IW664_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0847636.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            46388..48016
                     /gene="treC"
                     /locus_tag="IW664_000241"
     CDS             46388..48016
                     /gene="treC"
                     /locus_tag="IW664_000241"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0847637.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(48076..49257)
                     /locus_tag="IW664_000242"
     CDS             complement(48076..49257)
                     /locus_tag="IW664_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0847638.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            49509..51032
                     /locus_tag="IW664_000243"
     CDS             49509..51032
                     /locus_tag="IW664_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0847639.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(51101..51829)
                     /locus_tag="IW664_000244"
     CDS             complement(51101..51829)
                     /locus_tag="IW664_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0847640.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(51842..53123)
                     /locus_tag="IW664_000245"
                     /pseudo
     CDS             complement(51842..53123)
                     /locus_tag="IW664_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(53264..53866)
                     /locus_tag="IW664_000246"
     CDS             complement(53264..53866)
                     /locus_tag="IW664_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0847641.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            54111..54317
                     /locus_tag="IW664_000247"
     CDS             54111..54317
                     /locus_tag="IW664_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847642.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            54629..55348
                     /locus_tag="IW664_000248"
     CDS             54629..55348
                     /locus_tag="IW664_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0847643.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(55429..56112)
                     /locus_tag="IW664_000249"
     CDS             complement(55429..56112)
                     /locus_tag="IW664_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0847644.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            56330..58516
                     /locus_tag="IW664_000250"
     CDS             56330..58516
                     /locus_tag="IW664_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0847645.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            58862..59272
                     /locus_tag="IW664_000251"
     CDS             58862..59272
                     /locus_tag="IW664_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0847646.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            59286..59642
                     /locus_tag="IW664_000252"
     CDS             59286..59642
                     /locus_tag="IW664_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0847647.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        19383 a        10667 c        12134 g        18063 t
ORIGIN      
        1 gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttacaga taaacaatgt
       61 gttagaagta gtatcttctt ttacataaaa agctgaccat aggataaatg gtcagcgtat
      121 ttagtgagtc agctatttag tatgggtttt ctaaaggaac gactgtctct cttttgatca
      181 agcgatgagg gatgaccaaa cgtacgccac tgctcaattc ttcatggatc aattcgcgca
      241 gcttttgcat agcgacacgt cctaattcgg agatatcgat atcgatactt gtaaggtaag
      301 ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat gacggagagt gtctctggaa
      361 tgacatagcc atacatttgc gccaactgca tcgtgcgaag ggcgaagata tcgtcaatga
      421 ccaccaatgc tgtggcttcg ttttctttca acgttttctc aaaggacaga taatcctccg
      481 gatgctctag tactacactg gataacgcag gaagaccagc catcatcatt gctttttgat
      541 aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt gtttgtcaca aacaggatat
      601 tcttatgtcc attctggatc aaaaattctg ttgcttgttt acctagaagc tggttatcat
      661 tatctacata aacgatttcg ttctcatgat gataaggctg tccgatcaaa gtgaaaggga
      721 tgtggttttg gtataaataa tcaatgacag tatcttgact atctgaatac actagaataa
      781 aaccatctac ttgtttttgt aaatgcatac gctggacgtt ctctaacagc aattcaaaat
      841 ttttagcagt agcgattgct gtagtcatat cataattccg cgcttcttcg ttgattgctt
      901 ccatgatctc aagataaaat gggttaccga tccgctcttt tgaatcaaga gggggtaaga
      961 ttacgccgat ggcactggaa atgcgttttc ctagattgcg ggcagtcatg ttaggaacat
     1021 aacctagttc atccataatt tttcggactt tcttctttgt tgtctcggaa atactaggat
     1081 gatcgttgat gacacgagag acggtcgagg tggcaacacc ggcttttttc gccacatctt
     1141 ttacggtaat agccatgata aaaacacctt tctcttgtta ttgtgcagag aaccgataaa
     1201 tcgtctgact gatccgttct tgaccaggat ggaggttgat cgatccaaat ttggggaaat
     1261 gaacgatatc agggatttct tgcggttcga ttgctaatcc gtagtttgaa tgcatctgtt
     1321 ttccattgac agagaaatca gcttcgaatc cagtcgtaga aaataatacc atacttttat
     1381 tagaagttgc tatagtcatt tggcgaccag aatgcttctc gaataattgt aaggaagttt
     1441 tggaaagcct aggatgatgc aaaagaaata catcatctag tccatctgga taacatgcaa
     1501 ggatctcttg cagtgttggg gattttctaa agtcgattgg cagtttttct ttttttgtcc
     1561 aatttccagt aggcagtttt tcttgatcta attcaagcac accatctaaa aagaccgtta
     1621 actgatgttc atagatgtct cttgttccat ttcctgaaag gttgaagtag gcatggttgg
     1681 tcggattgca gatggtttca acaaggctac tagcagaggt tgtcatctct aattcgtttt
     1741 ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg ataggtagaa aattcatctt
     1801 ttaagtaaaa gaccacacct attgatgatg gagttttgaa aacttctact tcccaaaatt
     1861 ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga tccgcagttt ttctccaatc
     1921 tatgctcacc ccaagaagca tcccggatcc ttccggctac aggtccgaca gttgctccaa
     1981 aaaagctttt gtctgttaga acatcttcga aacgatcgta agacaataaa acattttctg
     2041 attttccttc tttgtctggt gcgaagattt ggtgcaggcg ggcaccgtaa ttgagaagaa
     2101 cgacttctaa gtagtcgttc ttcaatgtga ttttctttat ttcagtctct ggaactggta
     2161 cgacggagat ctctggatta tccatggtca tgccactttt ctttcaagta atcgatcgta
     2221 agaaaagtcg tatcagggac aagcgggatc tcctctccaa ggtcttcagc acgtcccacg
     2281 ccaattggtt gagcgccact tgagaggatg gcttggatac cggcttgcgc gtcttcgatc
     2341 ccgatacatt cttctgcttt tagtccgaca gcttttgctg caaggaggaa gatatctggc
     2401 gcaggttttc cgtttgttgc ttctgccggg tcggcaatag catcaaagta aggtgtaaga
     2461 cccatttttt caagtaggaa tggcccgttt ttacttgcag aagcaagagc aatcttgatt
     2521 ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg ggaagacatc ttttggttcg
     2581 atcgtctgga tcatctctaa ataataatcg ttttttcttt tcgctaattc tgcaaattct
     2641 tcctttgaaa aagagtgttc ttttccgcca taagctaaaa gcaaagagag tgagtcttct
     2701 cggctgacgc cttttagctg ttcgttgaat gtgtggtcga tcgtaatgcc gatttcctga
     2761 ccaagctttc gccaagcacg ataatggaat tcagcagtat cggtaatgac accgtctaaa
     2821 tcaaacaata cccctttaaa catttgcttc ctcctttctt agcgttattt ctagacgatc
     2881 cgctaaagaa taagactgtt catagacgta aatttctaga ggagaacctt ctaataactc
     2941 gaaagtaact tgttcatctg tgactgagat gaacaataaa cgtccgcgat aattgatatg
     3001 gaaagaataa gctttccaag tttttggcag aaatggtgcg aatctcaact gatcatgggc
     3061 agttttcatt tgagcaaatc cttggacaat cgacaaccag ctaccagtca tggaagtgat
     3121 gtgcaagccg tcttctgtgt cgttattgta attatccaaa tccaagcgag ctgttcgctg
     3181 gtacatttct acagctttgc tttccattcc taattctgct gctaaaatcg aatggatact
     3241 tggcgaaagg gaagactcat ggacagtcat tggttcatag aaatcaaagt ttttttgctt
     3301 ttcttctttt gtaaaagtat cattgaagaa ataaatcccc tgtagtacat ctgcctgctt
     3361 gatgaagcaa gatctaagga ttttgtccca agaccaatgc tgattcagag gaagatcgct
     3421 tgtgggaata tcggatacag gcatgagatc tttatctaag aatgtgtcat gctggacaaa
     3481 gattcctaat tcttcgtcaa ctgggaagta catgttctct gcgatatctt tccatttggc
     3541 tagttcttct tcgctgatag tgatagcagc ttctttttga taagttaaat aattttctcg
     3601 cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac caattgttat tgatattgtt
     3661 ctcatattcg tttgggccgg tcacaccgtg gatcatgtat ttatttgctc gttttgaaaa
     3721 atggacacgg tctgcccaga agcgtgcgat ttctgctaaa acttccagtc cttcattttt
     3781 caaatattcc gtatcgccgg tgtaattcgt ataattgtag attgcatagg caatcgcgcc
     3841 atttcggtga atctcctcga atgtgatttc ccactcgtta tggcattcga caccagtgaa
     3901 ggtgaccata ggataaagcg cgccttttaa tccttgttgc tgagcgttgt ggattgcttg
     3961 cggcagttga ttatgccgat atttcagcag attcttagtt acttcaggtt tggctaaagc
     4021 aagataaaga gggacagcat acgcttctgt atcccagtaa gtggctccgc cgtatttttc
     4081 tcctgtgaat cctttggggc cgatattcaa tcgttcatct tcgccgtagt aagtagagaa
     4141 tagttggaac aaattgaatc gaataccttg ttgtgcttct tcgtctcctt cgatgaccac
     4201 atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc tcttcttttg cttggatata
     4261 attgtttgtc tggttcgtta attctgagac agctttttct gcttgctgcg cttctggaat
     4321 atctctacta gtaagaacga taatttcttt ttccactgac catgtctcac cttgagataa
     4381 ttctgcagac aagcaaccat gtaatgctaa agtatcttcg tgatagtcag ggatgactga
     4441 ttttccatta acgaaatggc gcattgctgc agtaacagta aattgttcaa tcccaaagtt
     4501 gtttggaata gttttagtcg tcaaatagcc gatttcttct atagttccac gatcaatctc
     4561 attccaaaaa tgttcatcat agttgctgtc ttcgttttga acatttccat ccaattttga
     4621 tacgagtttg actgttccgc taccttcaag catttctata gttaaataga tataagctga
     4681 ttcttttttg acaatgctca gaaaacgttc gaaggaaatg cgtactttat tagtagaagt
     4741 agtgattgtg aatgtacgag ataatacacc atttttcata tctaattccc agtaaaagtc
     4801 ttctggttcc aaagaagcca agtcgatcag ctgatcgttg atcgaaatat ctatcgcgat
     4861 aaagttcaag gcgttgatca cttttccaaa gtattctgga taaccatttt tccaccagcc
     4921 gacacgtgtc ttatcgggat accagacacc cgccaaataa gtaccttgat ggtggtcccc
     4981 tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa ccgtttccaa tacttgttaa
     5041 agattcttgc agtcgaagat tttttttatc taattgtgtc gtgctgattt tccatggatc
     5101 aatagcaaat agtcgtttga tctgtttcat ttttctcctc ctaatgagct gttttatacg
     5161 ttcataataa acgcaaccga ttgcacaagt caatagacaa aagaaaatat ttcatagaaa
     5221 aaagcttaaa aaaataaatt tttaggagaa aattatagaa gggaacacac gtattatagg
     5281 ttttatagaa tggtatagac agaaaaataa aaaataaagc atttacaaaa aagaaaacgc
     5341 acgctataat acgtttaacg aaaccgattg cgtaaaggag ctaaacggaa tgaaaaagat
     5401 atttagtttt gagttttggc aaaaatttgg aaaggcttta atggttgtgg tagcagttat
     5461 gccggcagct gggttgatga tcagtatagg gaaatcaatt cccttgatca atcctgattg
     5521 gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt ggttgggcaa ttattggtaa
     5581 tcttcatatt ttatttgctt tagcaatagg aggaagttgg gcaaaagaaa gagcaggcgg
     5641 agcatttgca gctgggatct catttatttt aatcaatcgg atcactgggg ctatctttgg
     5701 tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc cacactctgt tcggtacgaa
     5761 gatcatggtt aaaggatttt ttacaagtgt attggaagca ccagctttga atatgggagt
     5821 attcgtaggg atcatcgctg gatttgtcgg ggcaatggct tataataagt actataatta
     5881 tagaaaattg ccggatgctt tgtcattttt caatggaaag cgctttgttc cgtttgttgt
     5941 tatcttatgg tcaacaatcg tggcattgat tcttgctgtg gtgtggccaa acgtacaggc
     6001 tggaatcaat aattttggtt tatggattgc agaatcacaa gaaagcgcac cgattctagc
     6061 accgttttta tttggaacat tagaacgttt actattgcca tttggattgc atcatatgct
     6121 gacgattcct atcaactata cacagcttgg cggatcgtat gagattttat caggcgcaca
     6181 ggcggggaca caagtatttg ggcaagatcc gttatggttg gcttgggcca ctgatttagt
     6241 gaatcttaaa ggtgcagggg atatgtcgca atatgagttt gtgttgcata actggacgcc
     6301 ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt atcttgatgg gaatggcttt
     6361 tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga tataaatcaa tgtatttttc
     6421 agcagcgttg gctgtctttc taacaggtgt aacagaacca ttggagttca tgtttatgtt
     6481 tgctgctgtg ccattatatg tcatttattc agtgatccaa ggggctgcat ttgcgatggc
     6541 agatatcttg ccattgcggg tccattcatt tgggaatatc gaattgctga ctcggacacc
     6601 gttagctatc aaagcgggtc taggtggaga tcttatcaac ttcgtgttgt gtgtgatcgc
     6661 ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc aaaaaattca actttgctac
     6721 accgggacgt aacggaaatt acgacaatga cagtgaagaa accgttgtat ctaacagcgg
     6781 aacaggaaca gcagatcagc aagtggtcca gatcattcat ttgctgggag gaaaagaaaa
     6841 tatcaaagat gtcgatgctt gtatgactcg gttacgcgta agtgtaaatg atcgagaaaa
     6901 agtagggact gaagaagcct ggaaacgagc aggggcgatg ggattgatcg tcaaagacaa
     6961 tggagtccaa gcagtttatg gaccaaaagc agatgtcttg aaatcagata tcgaagatct
     7021 gctgcaatca ggagcagaga ttccagagcc agaaatggtt tctacaggaa atggacaagc
     7081 aggtagtaag caatacttag gtatagaaca agaactagtt tccgcagctt ctggagaagt
     7141 gattccttta tctgaagtaa aagatccggt attctcacaa aaaatgatgg gtgacggctt
     7201 tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc gctggaaaag tgacaagtat
     7261 tttccctagc aaacatgcaa ttgggctgga aacgaaagat ggtatcgaag ttttgatcca
     7321 tatgggaatc gatacagtcc aaatgaatca gccagctttt gagattgctg tcaaagaagg
     7381 ccaagaagta ggaacaggaa cgaaattagc tgagatggat cttgatgtga tccaaaacga
     7441 agaaaaagat ccaacgatca tgatcgtatt tacaaacgat aaagtagaag aagtagtgat
     7501 caagcaatta ggtacagtga cagctggtaa agtgatcggc tccattaaaa tgtaaggaga
     7561 ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag ccaaacaaaa atagctcctc
     7621 ctatttttcg tttggttggg cttattaccg agaagcagca gtttgaatac tgtttagtgg
     7681 agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa cagccaaaca aaaaatagct
     7741 cctggtccat tgagcttatt actgaggagt tagcaggaga acactatttg ctggagaggt
     7801 tgtgacaaaa attttgtcac aacccttttg tttcgaaatg aagacaagga gaaaaccaat
     7861 gaaattcatg acactcaaca cgcatagctg gcttgagcca gaaccagaga aaaaattgca
     7921 agaattagca gataagatcc tgttagaaga ttatgaagtg attgctttac aagaaatcaa
     7981 ccaattactg gaatctgaag aagtagaacc tgcgaagcta atgaagttct gtccggtaaa
     8041 gaatcaagta cctatccgta aagataattt tgcctaccgt ttagttcagc ttttaaagga
     8101 acacggaaaa gagtattttt ggagttggga aatgagccac atcggttatg ataaatatga
     8161 agaaggaaat gcactgctta cgaaaaaaac attggaaagt caggtcctta ccgtctccca
     8221 atttcaaaag aaagaagatt atcagacacg caagatcttg attgggaaaa caaagataga
     8281 tgatcaggac atttttgtct cgagctgcca cttttcttgg tggacagata aaaaaagtgg
     8341 cttttatttt gagtggaaaa acttggaaaa ctactttttg gaaactcgcg taccactttt
     8401 tttcctaggt gatttcaata atccagtaga ctctcaaggt tattatactg taagagaaag
     8461 ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa aaaggaaaag cagctactgt
     8521 ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa ttgcgtatag attttatttt
     8581 cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt tttgatggga tcgatagtcc
     8641 aatcatcagt gatcactacg gggtggaaat cgaattagat gtgaacgaat aaaaaatata
     8701 acatataaca taaaaaaact gattaaaata aaactctcca aacgattata ccgtttggag
     8761 agttttttga tcggatgtat ttatcccttt tgcctaagca cgtaaaagct tgtatctatg
     8821 ataaataaat aaacatagca aggtaaacca gccaatcaaa gtaagagaaa gaatgaaata
     8881 gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg attccttttt gctgcttcac
     8941 gattggggtc ccgatatctg ttacttcata atcagtcaat gtttttccat tttgctgtag
     9001 cacagtccga tcgtagacga taacagggat atgaacagtt ttgtccccag tgtttttcca
     9061 cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa gttgtttcgt tttctagtat
     9121 ttcttcgtta taccggtcgt atttattgtc ttttgtctcc ttatagattg gcagatagtc
     9181 aggtgtggtt ttttggaaaa cgaacaagct ggtagacata tctttgctga agaatgtttt
     9241 ccttgcttct tccggcgtat cgaatagata agtatgtcga ctgacaaatg tttcattttc
     9301 ccacttttcc agatgctggt ataagttctg gatcgtctgt gctgaaaaaa taagaatgcc
     9361 aaatatactt aaaagacgga accacttttg ttcagaccac tggttcagtg aaagggcaag
     9421 gtaaagtaga agcaatagcg taaatggcac gaaaaatcga aatggaaact ggatcaaact
     9481 aataaaaggc acattttttt cgatcaatgt cgtccatgga atcaagctag tagaaagaaa
     9541 aaagaaaaca agacttgtgt acaacacgat tttttggaat aaggtgttct tttgttgaaa
     9601 gaaaataaaa agcaaaaaac ctgataatat caatatagga aaggccttag ggtaatggag
     9661 ccaataactg ccatccagat ctatcgcctt ttgactcatt tctctgttaa caaagggagg
     9721 aagaatctta ttcccactgt aaacaatagc taatcctgcc caaatatttg ctgtcagaac
     9781 aaaataaagc aagaccgact taatcagttg aagcagtgct tttccttttt cagtctggtt
     9841 aaaaaagtaa taaagataaa aagggatata catcaaagct aacatgatcg aagacagtaa
     9901 atggatctgt gtcataagtc ccactgagac agcgactttg ataacaggga actttttatg
     9961 aaggacgaaa tcagcaatag ggatcataca taaaggataa aaacaagtcc cccagctagt
    10021 gaatccttgg ttgatagacc agtattgaat agaatacgta ctcatgaata aaacggctac
    10081 tggtacactt acccaatttc gaacattgac atatctcagt aaaatcagca tagaaaatcc
    10141 tgaaataaga taaagaatga aatttgaaag aatctgataa tgaaaccagc tacctgcaag
    10201 caacacgatc aatccttgta aataagcgaa aaatgggccg taaacggcat tcacgatcct
    10261 gccactttgc tggaatccat aaagggaagg aaaataatgg aaatccccat atttaatttg
    10321 ttgcgcggca tcatagaacc gattgtaatg gaatggggca tcagcaccca aaattagttt
    10381 atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg aaaaaaattg ctaaaaatgg
    10441 gccatatttt ttcaattgtc gaactccttt gcaaacttta gataaattca tcatatatcc
    10501 tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa tatttatgaa ttttacaaga
    10561 aaaacataag tgaaagttct aaggagagag gatgcgacat aagttttgtt gcatctcctt
    10621 gttttgaaac agaatttttc ttaatactcg aaacaaagta gctttttcat gacctctcgg
    10681 taaacggtgt tcaatcagct gcttctcggt aataagctca ctagcctaga aaatatgaga
    10741 agctattttc tgactagcgc tcttattact caaagcataa cgcagttttc acaacctctt
    10801 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaacccaa caaaaaatat
    10861 gagtagctat ttttttgggc tgtacttatt acttgatgca ggacagcttt ttcacaacct
    10921 catcggcttt cattttctat ttttttcctg aacccatata ataaaagaaa gaacaaaaaa
    10981 acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa acagtctcgt cgacttttgt
    11041 ttcgtttgtt tttattaata tgtatcgtcg tagggttact gatcgcttta tatccatttt
    11101 atgtggattc tttaaatagt tttattgatc aaaaacgtat cgaacaagta caaaaacgga
    11161 ccgcagctga aattgaagtg cagcgaaaga aaatggagga gaaaaaccaa cgattaactg
    11221 accaaggatt caatcctggt actgatcctt tcaatgaaaa gaacagaaat gaaagtacaa
    11281 catccagcca attagaggaa tggctgatcg gaagtatcaa tatacctaag atccaattaa
    11341 atatttcact ttatgatcgg ctcaatggta ggatccttga aaatggagca ggcgtcttgc
    11401 aaggaacctc tttcccgctt ggtggaaatt cgacgcattc agtgatctct gctcacagcg
    11461 gtttacctaa tcgacggtta tttaccgaat tagatcgttt agaaaacgga gacacgttta
    11521 ttttgactgt tttgggggag aaattggctt atcaagttaa aaatatacaa gtggtactgc
    11581 cagatgacac gtctgttcta acgatttcag aaggagaaga cttagttacc ttgcttacat
    11641 gtacgccgta tatgatcaac acgcatcgtt tgctagtgac aggacatcgg attccttata
    11701 acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga aagaaccatg cggcaatggt
    11761 ttattcttgc aggaacgata atagctgtag tcatactgtt agtgtttatc ggacgactaa
    11821 tctatcagta tagattaagt aaaaaagtgc ttgatttttc atttattatt agtgattcgt
    11881 cagggaaacc ggtaaatggt agttcattca ttttgaaaca taagaaaaaa acacttacta
    11941 gaaatggtgt gccgttttca gtacaaagcg accatcatgg gaaagtcaag ctggatcaat
    12001 tgcctggtgg aacttatcga atcgtttcag ctgatgatcc taaagttgct gtttcttttg
    12061 gtattagaaa agtaaaacaa gagaagatat acttttttga ggggcgaaaa cttgtaaaag
    12121 agcttcaaaa aaatggtttt tattttaaaa tcaatgaata agtaaggaga gaaaaaatga
    12181 tccgcacaac aacaaaattg tctgccaaag aactggaatc tgttctatcg atttggctgc
    12241 atgctaactt ggattctcat gaattcattc catcaactta ttgggagaat caagtagaat
    12301 ctgttaggaa agctttacca gatgcagtga tttacttagc agaaatagat caagaaatta
    12361 ttggttttgc tggggtgatt gatcagttta tcgcaggaat ctttgtgaaa agggaatacc
    12421 ggcaacaaag aatcggtacg caactaatga aaaaaataaa agaaaccaat aaaacactga
    12481 ctttgacagt ctatcaaaaa aatcaatcag ctattcattt ctatctttca caaggtttta
    12541 caattcaaac acagcaaaaa gatgaagcag taaatgagga ggaacttgtg atgaaatgga
    12601 atcaaaacgc tgatttttga tgaacagaat aacgaaataa tgctcccagc ctaatttttt
    12661 gctgggagca ttatttcatt ttgaagtcac taatttaact gtgtgaatat gcagttgtta
    12721 caatctttga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    12781 aatatgagga actatttttc catttgtttc tctaatactc aggtcaaaac gctgatttca
    12841 caacctcttg ataaacggtg ttcaaacggc tgcttctcgg taataagccc actagcctag
    12901 aaaatatgaa aagctatttt ctaactagcg ttcttattac tcaaagcata acgctgattt
    12961 cacaacctct tgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    13021 caaaacatga ggaactattt ttccgtttct ttctctaata ctcaggtcag aacgctgatt
    13081 tcacaacctc tctttcacaa ccttatttta ctcgtttata attttcatcg aagtattttc
    13141 cgctacggat atcatctggc attccatcgt atggttcttc gctaataaca tcttcattgt
    13201 ctatacctgc atcacccata taagtgatcg tagcaaattc aggaacaagt aattttggat
    13261 aagtttcata tttttcacga gctgcttcca tttctgcgat atgttcgttt tggaaagtaa
    13321 cagtgatttc tggatgttct tcaacccatt tagggaaaaa gatcattccg tgaagtttct
    13381 tttcatttgg cataaagtcc aatgccacgt ctgtccctgt tggctctgtc caagtgattt
    13441 tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg gtcttttacc caacggcctt
    13501 tgaccattcc gccatggata cggtaatcaa cagtatggtc atttttggca taccattcat
    13561 attcccaacc attgtcatac gtatagataa agtgagttcc aataaaatct tccaattctt
    13621 taaattcttt catgtttaag catctcctta taaattaaaa catgtaataa actacatgtt
    13681 ttcgattact catttatgat agaatggatt taaagatttg tcaaggaggg aaaactgatg
    13741 gcaagaaaaa atacacttcg atatattttg ttagggttat taagtaagaa gaagatgaca
    13801 ggatatgaat tgaatcaatc gttcaaaaat gaaataggag aattttggca agcaaaacat
    13861 agtcagattt atcctgaact tgcaaaaatg gaagagcaag gaatcattca gcatcaagta
    13921 gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat taacagaaga agggaaggaa
    13981 caattggcag agtggattca tacacccacc aatgaactac cggttaatcg agacgaattt
    14041 gttctgaaat tgtatttcgt caaagacgtg aatgatccgg cattagtaga aattattcag
    14101 cagcagcgtg accttcatga agaaaaacga cttcatctat tagagcgaaa aaaaacgatc
    14161 tttcctactg aaaaagaaaa acatgaaaat tacggacact acctgatttt agcgcatgcg
    14221 atcaatcggg aaacagaata tgcaaattgg ctaacagatg tattagaaga aagtaaccga
    14281 aaaaaataaa aaaatatccg aacagtgatg gagaattcct ttctatatga aagaaacgca
    14341 gatcaccgtt cggatattct tatgttcaaa caggcctttt ttcaagtatt tatgtagcat
    14401 ctacttatta aaaagcttgg tacttaggag attagtggcg tttagagttc ttttaacaac
    14461 tgttcgtagt cagctttttc atcgacagga aggagcttgt aagttaattc gtctgtctcg
    14521 ttgtcatgaa tgatgtacat cttagcattc tcttctttga agttaccgat ttgtaaaatc
    14581 gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt ttgaaaaaac tttacggaca
    14641 tggtgcattt tcttttggcg gatttctgcc ttttctgcag cttttgacaa agtaaatccg
    14701 tcatctaaat attttttgat cagttctact tttatgactg taggtaaacg atattttcta
    14761 ggacaattcg catcagcttc gatcgaacga atgaatccct tttgttccca ataccttaat
    14821 tgacgtggag acacacctac gatatcactt aattcactga tgccaacaac caattgatca
    14881 ttttccaaca aacgtttaag ggaagcccca accatttttg tcactccttt tgtcctcttg
    14941 cttcccttaa ttatatccgt attaacttat gtgtcaagta ggttgacaaa tagcgaattt
    15001 aggactaaag tattctagta gtttcagaaa aaattcagaa agaaggagaa atatgagtaa
    15061 caaccaaccc gtagatattt acggaaaacc atataatcgg acacttttag ttgttgtctt
    15121 attaattggt acattttgta ccgttttgaa tcagacctta ttgacgacag cttttcctac
    15181 attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa tggttaacga ctggtttctt
    15241 gctagtcaac gggatcatga tcccaataac agcatggctg atcaataaat tcagttcaag
    15301 aaatttatat ctttctgcca tgacgatctt tttgatcgga acgattactt gctttacagc
    15361 acctaatttt agtacattac taatcggtcg tttgatccaa gcttgcggtg tgggagtatc
    15421 gatgccatta ttgcaaaata tcatgctatc tattttcccg ccagaaaaaa gaggatcagc
    15481 aatggggatg tcagggatcg ttattggact tgctcctgct ttaggcccta cattatctgg
    15541 ttatatcatt gataactacc attggcgtga cttgtttggt atggtactgc caatcgttgt
    15601 tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt attccattgt caaatcctag
    15661 cattgatatt ttatccgcta ttttatcaac gattggattc ggtagtttac tttacggatt
    15721 ttctagtgta ggagatgacg gctggggaag cttgaaagta attggtttct tagttgttgg
    15781 tgtgattttc atcgccttgt tctcatggcg tcaattacat ttggaacatc cattcttgga
    15841 acttcgtgta ttcaaatcat ctacattcac gattgcagct gttttagctg gtgtaacaaa
    15901 tatggcgatg gtaggggcag aaatggtcgt accattgtat attcaaaata ttcgtggaga
    15961 atcagcattc cattctggct tgatgttgtt gccgggtgca ttgatcatgg gggctatgat
    16021 gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa cgtttagcca tcacagggat
    16081 gttcattttg acagcggcaa ctgcaccatt tgctttcttg acaaaaacga ctccagtttt
    16141 atatatcgtg atcctttatg cgatccgtat gtttgggatc tccatggtca tgatgccggt
    16201 aacgacttct ggtatgaatg cattgccaaa taacttgatc agtcatggga cagcggtaaa
    16261 taatacattc cgtcaaatcg caagctcgat tggtacagct attttgatca gtgtgttgac
    16321 gaatgtgaca aaagcaaata tcccaagcaa atctgtattg gataatacgc cattagctta
    16381 taaagatcag gcaatcaatg ccacactgtc aggttaccat gccgcgttta tcgtagcgat
    16441 catctttggg attcttggct ttattattgc ctttttattg aagaaaaaag aacctgttct
    16501 aatgaaagcg ggggataatg catgattatt ggagttttga ttgtcagctt gcttgctttt
    16561 gctttaacga acgtgttcgc taaaaaaaca tggcaaacct ttttatcttt gattttcgga
    16621 ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt tgtccaatca ctttggaatg
    16681 gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca gtgctgattc ccaaggagca
    16741 gatatgcttt tgtataaagc tttagggaat ggaaaagaaa aagtgtatct ttaccgaaca
    16801 aatgaaaaac aagaaaaacc aaaagcaact ggtacggata acgaaacaaa taaagtagaa
    16861 aagacagacg gcgatgctga aaaagtgacg aaaactactt attgggaata caaaaacgac
    16921 atgtataaat tctggttcaa tattgctgat aacaatcacg aatatgacag ccgagtgaat
    16981 acattcaaaa tcccagagac atgggtagaa ttaagtacgg accaagcagc caagttagct
    17041 gaattagtga agaaacagca aagtacgatg gaaagtgaag caaaagctta tgttcaagac
    17101 ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca aagcagagca agaacaacgt
    17161 acgaaagcct tagctgctga attccaacaa caagcattcg caaaattagt gaaagaagca
    17221 aaaggcgaat aatattttga acaccgttta aggaagaggt tgtgagcctg ccgtttaact
    17281 ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgttgact attgtgactt
    17341 gtgaccgagg agttggcagg agaacaccgt ttattcgaaa aaaggaactg cgacaagact
    17401 tttgtcacag ttccttcttt tttttgctat gctatactag atgtaaaaaa taggagaaat
    17461 aaaatgaggg aaatttattc ttttgaatca aaaatctatg cttccgaagt cggcaagggc
    17521 ggggcatata ttatttttcc gtatgatata agaaaagaat tcggtgctgg aagagttaaa
    17581 gtccaagcaa cttttgacaa tgtgccatat gaaggcagta tcgtgaatat gggcgtgaaa
    17641 aatccagatg gttcagtctg ctacatcctt ggactacgaa aagatattcg taaaaacatc
    17701 ggaaaagaaa tcggtgatat tgtagcagtt acagtgaagc aaaaataaaa gaggttgtga
    17761 caaaagacgt gtcacaacct cttgataaac ggtgttcaaa agccagtttc tcggtagtaa
    17821 gtcaaattta tgcaaaaatg tggaaaccca tgtttacaaa atttttctta ctactcgaaa
    17881 cgaagcggct ttatcacaac ctcttgataa acggcgttca atcagctgtc cctcggtatt
    17941 agctcaaaaa cggcaaaata tgagaagcta tttttccatt tttttctctc atactcaggt
    18001 cataacgctg atttcacaac ctcttctttc ataatggttt ttcaccatct ttataataaa
    18061 ctggttcttt gatatggaaa tctatttttt ccaaccaatc taaaatcttg taacctaact
    18121 cttctgacat cggaaaagag gcgttgattt gtgcattgga gatatggtca acatggatac
    18181 cggaagtgat cgtacaactt cccggaacgg cagttttgat gatcacagct attttttcgg
    18241 ctaagacatc gtctttatgg aaccgaccat cgtggctagg gaaacgtaca gtttgcatct
    18301 ctgtatcacg agaaaatgta gtgactgttc cgatatgtgg tgtatctccg cctgttaaag
    18361 tgatcaataa gtctttaccg attactttaa cagcagcttg gatcgtgaaa cctagtttgg
    18421 ttacttcaac tatcgtttct ttttccatat taatcaccct gccaacgttt tgaatgctca
    18481 tttacaattt caaattgatc caaaagtttc attgtatggt gattaacaag atcttggatc
    18541 gtttgaggct gattataaaa agcaggaatt ggtggtatga tttgcacacc tagtcgtgct
    18601 aatttagtca agttttccaa gtgaatggca tttaatggcg tttcacgagg aacaatgact
    18661 aactttcgtt gctccttcat tgttacatca gcagcacgtg caatcaggtt gtcgcctaaa
    18721 ccaacagaga tacctgcgac tgttttcata cttgccggta cgatgatcat tccatcggtc
    18781 aaaaatgatc cgctggcgac agcagcacct aaatctttgt ttgaataatg gtaatcaaac
    18841 aggctttcga attcagttag tgataagtcg gattccagct ttaagttctg ttttgcccat
    18901 ggactgatga ttccgtgtgt ttctatcatc ggatattctt ttagtttctt cactaggttg
    18961 atggcataaa tggtgcctga agcgccactt acaccaatca cgattttttt cttccgttgt
    19021 gccaattgtt ccattttccg cactcctttt tatgatgtca aaacagttgc agatcattca
    19081 ttatttcaaa tatttttccc aatcttctac ttcttggaat tttgcacgta caaaatgttc
    19141 tttcatatcg aaaggaactg taccgtcaaa gatagttttt gcacttatcc cacggaaacg
    19201 aatagagcca ggattatatt ctggtaattc tgatgggtcc aatggatggt tcctcattcc
    19261 tgggatcacg atgatatctt tatctccttg gaaacgagtg ttgaaagtcc aaactacatc
    19321 gttcatgtcg aaaatatcaa catcttcatc gactaaaatg actgtcttca attctttgaa
    19381 ggctgataaa gcaagaatag ctgcttgacg ctgaatacct tcatccgctt cgttttcttt
    19441 acggatttgc atgatggtca tcaatttacc accacctgca ggaggattgt agacatttac
    19501 tacttttcct ggaatagctt tatcgaccaa ggcaagaata cttgcttctg taggaatacc
    19561 ggccatagat acgtgttctt cactgggacc aatcgttgtt tgcatgattg gattgtcttt
    19621 tcggtgagta actgctttga ctttcactac ttgtacagct ggattagcat ccccagtata
    19681 gccaggaaat tctggcattg cttttcctga gttcgtgttg atatcttctt gcattgtttc
    19741 atttggcatg atttctgctt caatgatgaa ttcagcacga gcaataccca cttcatcgac
    19801 tgctatacca tcaacgattt gaataggttc gttacgcaaa gctcctgcac accatagttc
    19861 attgtagcca agtggagtgg taggtggttc gaaagtcgtg ccaatggcaa ttgctggatc
    19921 taaaccaata ttgatagtga taggcattgg tttattcaag gcttcgaatt gtttttggaa
    19981 gtgaccaatg tggcgtccgc caggcatgat gtacataccg atcgtatctt tatcttctag
    20041 aaccatacga tggatcgtta catcgctcat tgttttttct ggatctgatc ctaagactac
    20101 acccattgtg atgtatggac cagcatcttc aggagtattt gtaggtgcag gcaaaatgtt
    20161 gcgaatatcg aagtctgcat cggtagctag atgaacgact tcttgtgctg gagcgtctgc
    20221 tttgtctact ttcacaggtt tgattggatt ttctacagcg tctttcaaga aacgaccaag
    20281 tgttcgataa tcatggtgca aaatttcacc tacacgttta cgactagcca ttgttccgat
    20341 gttcacacgt gtgccgggga aacctttgat attgttgaaa gtcatagttg gtccttcttg
    20401 agtcggacgt tttactgtac cgccagcacc gatataacgg taaacaccag ataattctgc
    20461 ttcaggatcg atttcaatat ctgtttcgtg atattgacct ggaagtgttt ttagttcttc
    20521 gatgactttt cttaaatcat acggttgatc tgtcatgaga gattcctcct tagttgtctt
    20581 tacctatcta tagtaaaagg tgaaaaagag gaaaacaatt caacttgttc ggtaaagtat
    20641 tctacaatgg aatggatgcg tttttttgat aatagaataa actatgatat acttatcact
    20701 aaaagaaagc gcatacttag tgttggagga aggaaagtga aaaaaatgga ggagtcattt
    20761 cctaaagcag taaaagtgga aaatatagcg aatattttga aagttacatt tgaaaacgga
    20821 gaagtaaaat acgtgaaatc tcattggaca gaagagataa ccgatgcctt gcaatttgga
    20881 aaaaaaggaa gaggaaaaag aaaaaatctt ctcgcacttt ctcgtaatat gtggattggg
    20941 actgaagtaa cgattgaagc agatggaaca gtattcataa atggaaaaga caggtatacg
    21001 ccagaagagt tgtggtacaa aggaaagaaa agtataccag aattataaaa aataattaaa
    21061 aaaaacagcg cgaaacaagg aacatattac ttcctcgttt cttgctgttt tctactattt
    21121 cagcgctctt atgactcaaa tctaaatgtg gttgtcacaa ccttattctg cacgcaaagc
    21181 gatagcggcg tcttttttcg ctgccattcg tgcaggaaga tgaccgccaa gcatcgtcaa
    21241 tatagtagag acgatcacta gaataatcgc atggacagga tttagttgag caacattttt
    21301 tagatccgtc atgttgtata agatgctatt gatagggaac gtagctagcc atgcaataaa
    21361 cacacctaat atcccagaag aaatacctaa aatacacgtt tctgcgtcaa atacgcgagt
    21421 gatgtctttt ttacgtgcac ctaaagcttt tagtacaccg atttcttttg tccgttcaat
    21481 cacagatgta taagtaatga tactgatcat gatcatactt gttactaaag agatccctgc
    21541 aaaagcaata aggacataag tgatagcatc catcaatccg ccagtcagtt cagtcatcgt
    21601 tccagctaaa tcagtataaa tgattttatc ttcatctgat ttgcctttat tgtaatcatc
    21661 tagataatcc aagatctttt ctttgtcttc aaaattgttc ggataaatca tgatacttga
    21721 aggcaagctg tctccaccta aataactaag caatgtttgt ttggcatttt cgtcaacttt
    21781 ttcagtagtt aagacattta caccactgtc tctttgagct ttgacgattt ctgattcttt
    21841 gttttcgttg acgatttgag tcgttaattg atcgctataa gcgattcctg gagataagag
    21901 attcattgta gaagaagatt tcacgcgtag gatccccgag atcttcaatt catcagaagc
    21961 attctgatac atcgcatcat aatctgtatt agggataaaa ttcccagttg gaagttttgt
    22021 atagtaagta ttgttgttta ctaatttgaa tgttgtacca acgatatcat caaaatcaag
    22081 tttttcgtct tctttcacat caaatccaag attcttcaat gcgttgatat tagtattatt
    22141 attgccgtct acgatcagca cgacatctgt tgctgaggct ggataacttc cggcaagtaa
    22201 agaataattg tcttttaaaa agttttcttt gctagtgtca agctgtgtag ggaaacttga
    22261 cactcccaca cctgtcattg ctgacatcgt actagataaa gaaagagatt ccgtatcagg
    22321 attttgattc gagaagctga caggctgtac tttgccattt acatcacgta aaagattgat
    22381 tcctgtagta cgggtaaatc cgatattatt acttaagtta ggatcgatgt ctgttacgta
    22441 atcgacgtat gtttgatcaa tcttattcgt gtgctgtgct ttatcttctt cgctgacttt
    22501 tgcagtaatc gttttgctgt ctggataatc cgctttgctt gcacccaatc cggcatcgtc
    22561 acgagtctgg ttagtcgtta ccttagaaat agtaatcgga aatttcgcca tcgtttcagc
    22621 ttgcgtgtta tcgatttgtt tttggaatcc agaagaaagg gacagaacga tcgcgattcc
    22681 gatgatccca atactggaag caaaggaggt caagaaagta cgtccttttt ttgttcggat
    22741 attgttgaag gaaagcttta acgctgtcca aaaactcatc tttgttctgc gtaagttaaa
    22801 ttgatcatct tttggtcgtt cgatgtgagg atgggaatct gttaagatct taccatcaga
    22861 aaattcaatg attcgatctg cgtattgatg ggccagttca ggattatgtg tgaccatgat
    22921 cactaatttt tctttcgata actcttggat caattccatg atctggatac ttgtttctgt
    22981 atctaacgct ccggttggtt catcgcacaa aaggatatct gggtcattgg ctagtgctcg
    23041 agcgatggcc actcgctgca tttgtccacc agataactga tttggctttt tatgcatatg
    23101 atttgtcaag ccgactcggt gcaatgcatc ttctgcccgt tttcgttttt cgtctttaga
    23161 aacaccactt aatgtcatcc caagttcaac gttttcaata atgcctagat gaccaatcaa
    23221 gttgtaactt tgaaaaatga aaccgataga gttgtttcgg taggcatccc agtcactgtc
    23281 cttaaagtct ttagtagact ttccgttgat taccatatca ccggaatcat aattatctag
    23341 tccaccaatc acattcagca tagtcgtttt accagaacca ctaggaccta aaatcgctac
    23401 gaattctttt tgtctgaaag cgacagacac accatccaat gctttggtgg tggtttcgcc
    23461 gactttataa tattttttta tatcttttaa ttgaagcata atcacccttc tcccgtattg
    23521 aaatattcgg tatactaata agtatatcag aacaatgatc gtagtatacg aaataaatgt
    23581 taactgaaga tgaactacaa gaaaaaatct agaaattttt caaaaaacag agggagatca
    23641 atgaatcgta ttttagtagt agaagatgat ccgcaattac agttgcttta ccgttctgtg
    23701 ttggaacgag cgggcttttc agtcatcacc gcattgaatg gagaggatgc gctgaaaaaa
    23761 ctagaagcga cacgtataga attaatcatt acagatatca tgatgccgaa aatggatggc
    23821 tatgacttgc tagactcatt acgtagcacc agatttgaca cacctgttct aatgattacg
    23881 gcaaaggccg attttgagga taagaaacgc gggttcaaat taggagcaga tgattatatg
    23941 acaaaaccca tcgatgtcaa tgagatgatc ctacgtgtgg aagcattgct tcgacgttca
    24001 aagatcaatt acagcagtag cttgataatc ggacaaacaa aattagagct agatgcttat
    24061 acagtgttac aagatcagca ttcgttgatt ctgcctcaaa aagaatttct gcttctgtat
    24121 aaattactgt cttatcccaa caagatcttc actcgccaac agttgatgga tgatatttgg
    24181 ggattagata cgaatacaga agaacgaacg atcgatgtac atatcaaacg tttaaggacc
    24241 cgttttgaaa agacggaaga ttttcaaatc atcacagtac ggggactagg ctataaggcg
    24301 gttatttaaa gatgaaaaaa aagctatttt cacaattatg gatctatttt gctagtattg
    24361 tcttcatttc gatcttagtg acactgctgt gttttctcgg ttttattttc ttattgtctc
    24421 gacattctat ctcgcctgct gaatcggctg gaaaaccaac gttatacccg ttattcgtct
    24481 ttgcggggtt cagtatggtc gtgggtacag gaatatcgat ttttgttggg cggcgtatcc
    24541 tgcatccaat cagtgcgcta gggacaaata tgagtctggt agcaacggga gatttttcga
    24601 ttcgaatgga tgaacagcag aaagtagcag aagtacagca actgtataaa gatttcaacg
    24661 ttatggtcca agaactcaat agtatcgaga cactgaggaa tgactttgtg tccagtgtat
    24721 cccatgaatt caaaacacca ctagcaacaa tccaaggata tgttcaattg ttacaagccc
    24781 caaatctctc tgacgaagaa cgccaaatat ttttatatcg gattatcgaa agtatcacgc
    24841 agctttcaca attaacagag aacatcttaa aattgaacaa actggaaaac cagcgtatcc
    24901 aactggaaaa gaaagagtat cgattagatg aacaaatcag agaagtaatc gttttttttc
    24961 agcctaaatg ggagaaagaa cagctagaat tggatatcga acttgctgca gtaaattaca
    25021 caggcaatga agagtttttg taccaagtgt ggctgaatat catggataac gccataaagt
    25081 acaatcaaat caatggacag attcatatca aactatttga gacagccaca gagattgtac
    25141 tggaagtgac cgattcaggt gtaggaatga atgaagagac cagagatcga atgtttgaaa
    25201 aattttatca aggagataca agtcgtcaaa tttctgggaa tgggctagga ctttcactgg
    25261 ttaaaaaaat tctagaactg catgatggac gtattgatta tagcagtatc gaaggagtag
    25321 gaacgacagc aatgatacgg ttaagtaaac aataggaaaa agaataaaac ccctaaaaat
    25381 tctattgaag gtttttaggg gtttttagtc gtttttttgt gattagggaa atgttcttac
    25441 ggttcttttg gcataaaaat cgctgctttc ttattgaact acgagaaatg ttagaggtgt
    25501 cattgaaaca aggacatgat gcaagaaaga gagcgaaagt catgcgtcgc tctctttttt
    25561 tgggtggata gatggacaga aaatgtaggg cttagttttc ttcttgattt aaatactccc
    25621 aaaatgtatc tgaagccaaa gaatgctcac cgactttacg agtaaatgca agataactct
    25681 ctgttgttgg agaaataggg agaatcacag tattttcttt taatagattc ttgactgttt
    25741 tttccataac aatagcaatc cccatgtcat ttgcaatcat attgatgatc aagtcgattc
    25801 ttgctccttt ataagagatt tttggttcaa atccagcttc gcgacacaga tctagtacag
    25861 gttgatacaa gttcgtagtg gggccaagga gcaaaaagtt ctcctttttc agttcgctta
    25921 gatccaattt ctttttacta gctaaaggat gatttttagg tagaactgct acaaaatcgt
    25981 ctgtttcagt aggcaaccac tcaatcgaac ttggtggggc ctggaaactt ctagcaaagt
    26041 ggatcgtgtc actatgttca ttggcgtcat tgtaagttaa ttcgacactt tccacttctt
    26101 tcaactggac tgtaaattca ggatggcgtt tcaaaaactc ggtgattttg gaaaaacctt
    26161 tataattgat cattgtcggt atcgttaaga tatttaatga aagattgttg tctttcgtat
    26221 aggtttcaag cacttcttgc atagcatggt attgttctac gacatttttt acatatggga
    26281 gcaataagct tccagcttcg gtcaattcga tttttctatt ggcgcgttcg aataatttga
    26341 cgtctaattc tgtttctaac gaaagaatct gtttagaaat atttccttgg gtggtgaatt
    26401 gtcgatcagc agttttggtg aaattcattg tttcggctaa atcaagaaaa acttcgaatt
    26461 tatggatatc catcatattc ctcctactta ttccaataaa gaatacttta acacaaaaaa
    26521 tgaattggtg taatcttctc tgtctgttac aatctttctt gcagcgaaag caataattac
    26581 agaaattttt ctgatttttt tcgcttcgta gaaaaagaga ggagtttttt gatgccaact
    26641 ggagttttga tcaatgtagg ttccgttttg tttggcggac taattggcgg attagtagga
    26701 aataaattaa gtgaacattt taaagcacag ctgacgatgg tttttggtgt gtgttcgatg
    26761 gggatgggaa tctattccat tgcgccgatg aaaaatatgc cggctgttat ttttgcttta
    26821 gtgataggaa cagcgattgg tttgattgtt catttaggta atgggataaa taagggggct
    26881 gcgctgatgc aagtacctat ttctaaaatc ttccctagtg aaaagcttgg tatgacccac
    26941 gatgaattta tcagcacatt agtgacggtc attgttctat tttgtgcaag tggtacgggg
    27001 atctatggaa gtttagattc tgggatgaca ggagacagta cgattttgat ttctaaatct
    27061 gtccttgatt ttttcacagc agctattttt gcgtgtaatt tagggtatgt cgtttctgtg
    27121 atagcaattc cccaattcat tattttttat attttgttct tattggcaaa atttatttat
    27181 ccattgacta caccagacat gatcttagat tttaaagctt gcggcggatt cttgatggtc
    27241 gctaccggtt ttagaatgat caacgtgaaa atgttcccag tagctgacat gatacctgca
    27301 atgatcgtta tcatgccact gagctggatg tggacaaatt ggattatgcc actgttataa
    27361 taaaaatcaa agagaagcca ttttttgctg actaaaaagg atcaaatctt ttagtcagtt
    27421 tttttgtatt tgacaaactg attttgaaac tatattatat gttgtataat atagattgaa
    27481 ataaaatatt acacttgaga atagaaggtg acgtaatgca catccagatt ccaactgttc
    27541 ttcttgatgg tactgtgcta gcagcattaa gcaaggaaga tatgtatggg tatgccttaa
    27601 caaaaatcgt acaagaaagt ctctctgtca gtgaatctac actatatccg gttttgagaa
    27661 gattgaaaaa agaaggatat cttgagacct atgatgaacc ttttgaaggc agaaaccgtc
    27721 gatattactc gttgacacca atgggaagtc gtcatttaga agaaattatt gatgaatgga
    27781 ttgtttttcg agattcttta gaccggttat tggaggagag cacaaatgaa tgaatatttg
    27841 aatgaagttg tggatcatct tcaagaaatt cctgaagacg atcgttttga tttgatccag
    27901 tattatgagg aatatttttt agattctgga aaaacattag atcagatcat tgatgaatat
    27961 ggtacaccaa agcagtttgc cttaaaatta aaaatcagtt acttttcaga agaagaacaa
    28021 gcagaaaacg agttagtgaa aaatccaaaa agacaaattc gcctgatttg gttaatcatt
    28081 atcggtttat ttgcttctcc tgtcctcatt ccactagcac tggggtttat attggttatt
    28141 gggggactat tggttgcttt actagcgctc atttttagta tctatgtttt atgtatcagt
    28201 atcttaggtg ttggcctatt catgatcatc tcaggaatca gtgtgtttgg acaatcgatt
    28261 ctgagtggat tattctttat cggtgtcggt ctgttcgcca caggaagcgc agtatttatt
    28321 tcgccactgt tgctgaaagg aacaaaaggg cttttttatg tcatgatgaa ctttgttaaa
    28381 tggatgggac gacgttttat tacaaaaaga gatctgcatt cagcgggcgt ttgaaggagg
    28441 acacataaat tgagattgaa acattttttg attaccggta ttggtctgat gcttactggt
    28501 ggcctcctag caggtgcaac ttatacatta ggcgctcaaa agagtctgac ttgggataat
    28561 ggtcctcggt tgatcgatat ggttactgag acaaaaaaaa tcaaggatgc aaacaaaatc
    28621 gtcatccatg cagaaaatca aacagtttcg gtcatacgcg gattggattt tgagataaaa
    28681 acgacttatg ataagacaga aaagccagaa ataaaggaga aaaatggcgt gttgactgtg
    28741 aatgcagtca aaaaatcaac aggtgcaatc attagtttag aagacacaag tgcacaaatc
    28801 actattgttg tccctgcaga tcatgaatta tctgaattga caatcgaagg gaataatagt
    28861 gcaattgata tcaacgaagt atcaatcaaa aaagtgaata ctaagacaca tgatggctgg
    28921 gtatcgttca atgaatttac aggaggaaca ataactgcga aagatgaatt aggctcactt
    28981 cgattagatg aggccaatgt ggataacttg atcgtcacag gtgaaaacag caacttccat
    29041 ctagaagaca gtttgatcag caaagaagga aaagtcatcc taaacggtgg caatttatct
    29101 ttgcatgaga tggaaaacag tggctatact atccaaacgt ctggtaaatc ttatattgaa
    29161 aaagactacc agccaataac aaatactttg ttagaacaag gacagaaaaa gctaaagatc
    29221 actgctaaag acacatcgat caatattacg acggatcaag aggacaatga aacagattga
    29281 tcctgtactc aactggatat tttaagaaaa aacagcaaag atccttccaa tctttgctgt
    29341 ttttcatcgc ttaacgcaca taactatagt aaaataagaa agaaaatatc taaaaagaaa
    29401 acagggtgaa agagtatatg aaggtagaag aaggtcaaag agaaaagtta aaaacagaaa
    29461 tggaaagatt acatacttat atcactcaac taagccaaac cttctatgat ccagacaaag
    29521 aaaaagtgat ggttaattac ccgaacaatt cagaaggtcg ccaattagag caagtgtacc
    29581 atgaagtttt caaacattta ttgacggtga agaaagagct ggattattat tctttaccga
    29641 ttatcgatac aggaatattg aagtatgatg ggaagaagga acgatttatc tttaaatctg
    29701 ttcgggaaga tttgcctctt tcagcaggga tggatctaga ggtcttggta gaagactatt
    29761 ttacagaaga aaagcactgg gttcgaacaa aattggatta tttaccgcag gcagccggag
    29821 ggacacaggc aagcggttgg tacatcactg aagacaaaga gttagaacta gaaggtgtaa
    29881 tggcgcgtat tcgaaaaaaa aattaaaaaa gtaacattag atatttattg aaggataaag
    29941 gagcgcagga agtagcatga gagaaaaaag agcattttat atcaatggtt atgcagggat
    30001 tgtcggattg gtcgtattag ctttagttgg attgtttttc ttctatttag gtatgtggca
    30061 agcgaaagtt tgggcacttt ttttaagtat ttttctttgg ttgatcacgt tattgttgtt
    30121 aagttcggca actgtagtca gtcctaatca agcaaaagta attttgtttt ttggacaata
    30181 tttaggtacg attcgagaaa atggcttttt ccttacgata cctttggcac aaaaaatgac
    30241 tgtttcgttg aaagttcgga atttcaatag ttctgtttta aaagtcaatg atttggatgg
    30301 aaaccccatc gaaatctcag cagtagtagt ctttaaagtc gttgatacag caaaggcatt
    30361 attcgatgta gcgtattatc aagattttgt agaaatccaa agtgagacag ccattcggca
    30421 tatcgctagc caatacccgt atgatacatt caatgagaat gatttgacgc ttcgaggaaa
    30481 cacgacggca gtgtctgacg agttgcaaaa agagctgcaa gagcgtttag cagtagcagg
    30541 tgtcgaagta atcgaaacac gcttgaacca tttagcctat gcaacagaaa tcgctagtgc
    30601 catgctccaa cgtcaacaag caaaagccat tttgtctgct cgacaaacca ttgttgaagg
    30661 agctgtcaca atcactcaaa tggccttaga gcaaatcgaa gatggacaag aaatcaattt
    30721 caccgatgat cggaaagtcc aattgatcaa taatttactt gtctcaatca ttacagacaa
    30781 aggaacgcaa cctgttatca atacaggcga catgaaagac cgtaagtgac atactttttg
    30841 taagatagga agaaggagtt taggacggat tattgtcttg aactcttttt ttagttgttt
    30901 taaagaaaat gacaaaattg aaagaagaat gatagttgat tcgaaggcag aattgttcgc
    30961 ttttttctat aatcagtata taaagtaaga gagaagggga aaaagtgaaa aaacttctga
    31021 tcggtctact tgttttcatc gttgtggtac ttgcaggggt gaaaatcgca gattacgtag
    31081 ttatgggtgg agaagaatac tatgtacaaa ttacgacaga tggtacaaaa acagtttcaa
    31141 aatcagactc tggacaagaa acagtcaact atagatatat cctgcctggc tatacgaaag
    31201 agggtaagga aaaagaactg gacttcaagg gacaattgac tcgaccgctt cgtaaaggag
    31261 catatttaaa aatcacctgg aacaaaaata aaggtgtcac ttcgtatgaa gaagtcgaaa
    31321 aaaaagacgt gccgaaaaca gcactggaaa aactaattaa agggtaggga aagaaatgaa
    31381 caaaatagtc gaagtacaga atgtaacaaa agtatacgga aaaaacaatg agaaaaagac
    31441 gcaagcatta agcggcatca gcttcgatgt agaaaaaggc gaatttattg ggatcatggg
    31501 cgcttctggt tctggtaagt cgacactttt gaatatttta tccacattag ataaaccaac
    31561 agatgggcat atccgaatca atcaaaaaga tgtgaccaca ttaaaaggaa atcaactagc
    31621 agattttcgt gcgaatgaga ttggctttat tttccaagat ttcaatttat tagaaagctt
    31681 gacagcacaa gaaaatatcg ctgttcctct ttctttacaa ggcgtcagac caaaagaaat
    31741 caagcaacgt gtccaaaaaa ttgcagaacg tttatccatc tctcatattt tggaaagcta
    31801 tccttctgag atttccggtg gacaaaaaca acgggtcgcc gccgcacgtg cattgatcac
    31861 acaaccaacg attctattag gggatgagcc tacaggtgcg ctggattcca aaagtgcccg
    31921 ggatctttta gatacgatgg acgaattgaa tacaaaagat catgtatcga ttcttcttgt
    31981 gacgcatgat ccgttttctg caagttattg ccaacgtatt ttattcatca aggatggcgg
    32041 tatccatcaa gaagtgaaac gtggagatca aagccgtgat tccttctatc gagaaatact
    32101 atctattctt ggaaacttgg aacagtaggg ggagattgac tatgctttgg aaattatcac
    32161 ttactgggat caagagccgt ttaagagact acttggtgtt attctcagga ttagttatgg
    32221 catctgctat tttttacatg tttgaatcaa tggcaagcaa cgaagctttt ttgaaaagta
    32281 atacgatcgt tgcggctaca gttatcattt tccgattcgg ttctgtttta ctagggatca
    32341 tcacattcgt ttatatttta tatgcaaatt cctttttgat gacgatgcgg caaaaagact
    32401 atgcaatgtt tatgatgctg ggagcaaaag gcagaaaaat cgcacagatg attttttctg
    32461 aaacattctt agtagggatc gcagctactt tagtgggttc agctgtcggt gtaggattga
    32521 caagtatcgt ccaccgatta ttagtggatc agttagatct caatatttcg cattttacac
    32581 cttttagtat tcacgggttg ttgatcactg ttttgttttt tgctgttttg tttttattag
    32641 ctgctgttgt caatgctttt tcgattgtca aaaaaccgat tttagcattg attcgtgcgg
    32701 atcagacacc aacaagaatc aagcagcata aattcttgtt tttccttgaa gtagtactgg
    32761 gaattgcttt tctaggctgc ggttactata tgatgaaaca cgtggggacc tatcaagtct
    32821 ttggtatagg ggtagcctta gttacgatcg tattaggaac ttatttcatt ttccattctg
    32881 tgatcatttt ctttttatca ttactaaaga aatcagaaca gatttcattg aaaaaattga
    32941 acaatttcac attgtcacag ttaaactttc gtattcgtga atatacacaa atgctctcaa
    33001 tggtcgctat gctgtttgcc ttggcactgg gcgctttgac ggtaggatta ggttttaaaa
    33061 atgaaattgc agacttgaca gaaaacaatt ctggatatga tctagtgatg aataatcctc
    33121 gggcagaaga tcagcaaaaa atcaaagaac tttcaccaac actttctgct tcttatactc
    33181 aaaaagaaga tgcgggaacg atttactata atgcaagtga atttgcggct caaccattga
    33241 tcatgataaa acatgaagat ggaacaccgc cgaagatcac gaaagaaaaa gttcctgcta
    33301 aaaaaatgaa tgaactgact gtacaagagg aacttcgaag ctatgaattg ccggaacagc
    33361 aagaaaaaga aatcaaggta gtcagtcaat cagaatttga tcaactgaac ttgccacaat
    33421 ccactttaca gctagttcag gtaaaagact tccaagcaaa cctgaaacca atcgaaacac
    33481 ttgttgctca aaataaaatg aacaatccat ctttgcaaaa agtagagtac agcaaccaga
    33541 aatatgaaat gtatgaagtg tataatggtc tattctctgg ttttgaattt atgggattct
    33601 tcctaggttt agctttcttg accatgcttg ctagctgttt gatgttcaag atcttgtcag
    33661 gaagcaagag tgacattgct cgttatgaaa tgcttgaaaa gataggaaca cgacgcgggt
    33721 tgcttaaaca atcgatccgt agagaagtag gcgtgttatt cttagcacca ggaatcttag
    33781 gtgtagtcca tgtcttattc ggattacaaa tgtttggtct attcatgcaa aatccataca
    33841 aagatatttg ggttccgttt acgatattct tcgtattgta tttcatctac tatgtactga
    33901 cgacatggtt atatactgga atcgtattga aaaagagatt gtgaaaaagc tgtcctgcat
    33961 caagtaataa gaacagccaa gcaaaaatag ctcctcatat ttttcgtttg gttgggctta
    34021 ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa aaagaggttg tgacaatatt
    34081 tttgtcacaa cctttttttc acggtctttt attagaatat agtaaatttt ttctcgttac
    34141 tcacggaata agctgttaag aaaacggttt ccatgtatgc tggaatcagt gcaaatgaaa
    34201 gaaaagaggg tgcaaggaac atggaaatca atgaacaaaa ttttggcgaa aactatcact
    34261 tgattacgat cacgaatcaa aatgggacat ctttgtctgt cagtgatctt ggggcacggg
    34321 ttgttcattt tcaaacaata atcgataatg aaaaacgaga gttgatctta ggttttgatg
    34381 aagcaaaaga atatctagaa aaagaccctt atatcggtgc ttcaatcgga cgtatggctg
    34441 gacggattac gaatggcaca ttcaaattag atgatgaaac gttcgaagta aaaacagacc
    34501 cggacactgg tcattggttt acatggtggt gcacctagct ttgaagccaa gcagtggcat
    34561 tatacagtca tcaatggtga aaacgaagca tcggtcattt tctatcagac tagcccagat
    34621 atggaaaacg gatttcctgg aactttagat gtagaagtac ggtatacgct gacgaacgat
    34681 gatacatggc gagtgacggt ccaagggatc agtgaccgca aaacagtatt caatcctaca
    34741 aatcatgtct atttcaattt aaccggtgat ccagcacagt caatcgacga acatatactt
    34801 tggttgaata gccgattatt tgctacgctc aataaagata caacgcctac aggagaagta
    34861 gtttcagtaa gtggcacagc atttgacttc caacgtccta aaaaattagg tgaggtcttt
    34921 gccagtgatt ttcgccaatc aatgatagtg ggcggcatcg atcatccatt tttgttgaaa
    34981 aaacaatctg gtttactaaa tgctgcggaa ttgacaagcc cagatgaaaa agtatcgatc
    35041 caagtaaaaa cagatgcttc aggaatcgtg atttttacag caaattttgg agaagatgga
    35101 ccggaaatgc gcgggaataa attagtaaat catggcggta tcacatttga gactcaagaa
    35161 ctgccaggag cggagcgact tgaaagtttt ggagatgtca ctctggaacc taaccaagta
    35221 cgcgagtttg taacggaata caaaataaat ataaagaaat agaataaatt aaaaagggag
    35281 tgtgacagtg tcacatcccc tttttaattt attctatttc tttaaaggca gatcaagcca
    35341 gaatgatttg tctaaactta acaaggttag tacaggaaca gttgcagctt taactgtacg
    35401 agtagttgtt tctggtatct gtcttgccgt aaaattgttc cacaaatccc agattgaagc
    35461 gacacggaag atggaagaag cgttgttgac gcgttttatc gttgaaaatt ctaatcctgt
    35521 gattggcacg ccatctgttg tccatttgat tggcacatcg aatcgcttag acatattgag
    35581 gatttcagcg tttgcttctt cagttgttgc tgcttctttt ttcggttcat catattttgt
    35641 caggtcgtac gcttcgatga tcgcgttcaa tttttgtgaa tattggcgat ctgtagcata
    35701 acgtccttcc aaatatttgg ttgcttcttt gtagtctttt gtttccgact tccaagttgg
    35761 tttgtagaaa tcaggattgc cagtcagtcc atttttgatc aagtcggcat agtcttccaa
    35821 actttccttg taagaaggat atttacggaa ttgtgcgcgg atttggtagc tttgtccgga
    35881 actatcttgt tcaagtgtat tgaaggtcac gctgcttcct ttatatgatc ctttgatacc
    35941 aaaaaggtta tgatttggtt cgcttgataa taagctgttt ccagatccac tttcaaggat
    36001 agcttgagcc atcatgactg atgcgtagat tccttctttt tgcccgattt cctgtgcatc
    36061 atctgcaatc tttttcaaga actgatcagt tgtttggttt tgactgaaat ggaacgagcc
    36121 atctaatcca tctaaaatag atgagccgcc tgtcagcgaa cctgaagcag caggtaaacc
    36181 tgctaagttt actcggacag caaaatcagg cgtgaaatct gaaatagaag ttactcgaac
    36241 tttatctcct ggttgaggcg catggataaa ttgtccgttc ccaatataga tacctacatg
    36301 gtgagtaaca ccggaagaag gtccccagaa gacgaggtcg ccaggttggg cctgacttac
    36361 tggaatctgc gtacctgcat attgctgttc gcctgtccag ccaccgatat aatgaccggt
    36421 tgctttcata tatacgtaat aagtcaaacc agaacagtca aaagtatttg gaccttttgc
    36481 tccccataca tatggttttc caagatgctt gaatgcttct cgaacgattg cttgttggac
    36541 attcgttgta tttgtaggca atacagcatc aattggtgat tttacaacaa aatcagaaga
    36601 agcagaagat ggattctcta taccgctttg atttgctgta tttggacgag atgaagcatt
    36661 gttcgttccg ccattcgatg aaccattgtt tggatggttt ggttctctct gagtatttcc
    36721 attgttattc gatccagatg ggtttgtttg attcgaagaa ttcccgctgt tcgaattatt
    36781 gttattattg tttgaattat ttgaaccatt attattgttg ttattaccca catctggttt
    36841 gtttggcgtt tccggatctg gtgtactagg gacttcttca ctaggatcag ttggtttctc
    36901 tgttgaagta tccggtccag ctttaaattc tgcagtaata gataaattgt tcgtacctac
    36961 tgtaaaggtc tttgcatcat aatttacttc tttggcacca ttaacagcga ctgaagcaat
    37021 ttgttcacct tcagctggcg taatagtgta agttaccttc gtttccgcta ataaaccttc
    37081 taccgttcca ctttccgtaa tatccaaagg ttgctgttca ggggaatccg ctgtcgaagc
    37141 agtcagttgg atcttaccaa tcaaagaagg agcaattgta agggaatatg tctcggctgg
    37201 cttttcattt tctgatggat cagccggatc aatttcagtt ggctcggtcg atcctgttgt
    37261 tgagttagtt gttccagttg ggtcttctgt aaaagaatcc gttgtttcac tagtagctga
    37321 gctggatgta gtcgtatctg ttgtgactgt ttgatcagct atttgattat ctacggcaaa
    37381 tacggtattg gtcgacaaaa cagacggagc aatcagccaa gtagcagcta gcaaacgtat
    37441 cgttttttta tttttcataa taaactagtc cttttccttt cattgatttt ctgtcttatt
    37501 cagcattcat tagacatttt ttggaacaga taatcgtgtc gaaaagcttt ttcttttcat
    37561 aaatctgtct gtgtattcta aataaaacaa tttgtaaata agcaacgagc attcattcgt
    37621 ttttctttga cgttcatttt attgcataga ccacctttgt ttggggataa acaagtgggg
    37681 ataaaaatgg ttatgcatat gtaaattata ccaaagtttg gaaagtatca caaaaattta
    37741 taaaaataat gatcctaaaa agaaaaagac tgaaatatgt gataaacatc tcatacttca
    37801 gtctttttca ttaactattc ataagacatt aggtcttcat caaacccgct aacgagagta
    37861 gtgatcattg ctacgactag actaacagcc agtcctgcta agtaccagaa aaaagtaccg
    37921 gaaatgaatg caggcaatgt ggtcagacta ccaaatacaa aagcgatcgc tttcacttgg
    37981 gcaaatccca ttacagctcc accagcagct gcaccaatgc aagcagcaac aaatggtttt
    38041 ttcaatggga tcgcaattcc gtaaacagtt ggttcggtca ctcccgcaag taaaatcgaa
    38101 gcaaaagcag aaccggagat cgaacgggtt tttgaacgtt tacttcgcag aaatacacct
    38161 aaagctgcac cagccattcc aaaattggct gctcctgtca acggaacgat tcggtcgaac
    38221 ccataagcag agatattact gatcatgaca gggtttacag cccattggat accaaatatt
    38281 accaaaatat tccaaccacc agcgatgatt gcgccactga tcggtccgtt aatatccatc
    38341 atccaagtaa ttgcggcagc taaaccttcg ccaaggtaaa cacccgcagg cccgaatacc
    38401 attaacgtca aaggaaccgt gataactaat gaaagtaaag gtacaaatac taattgcata
    38461 ttttcgcgaa tatatttttt gaggaatttt tcaacaaaag aataaaagaa aatacctaaa
    38521 atcgccggca tgacagtaga cgtatactgc atcagtacta caggaatccc cagaaaatca
    38581 gtagctgctt tgccactgtc tgaaagtaaa gaggtcagat tcggttcaat cagtgctgca
    38641 gcaatgacag ccgcaatata tccattcact ttaaacgttt tggcagcaga aaaagcaaga
    38701 aagattggca gaaaataaaa gacggcattc gacgcagcag acaaaatatg ataagttccg
    38761 cttgtttcac taagccagcc aatcgtagtc gctaaaacaa ccagaccttt taatatacca
    38821 gaaccggcaa acagcggaat gatcggcgta aagatttccg atatcaaacc aaatagtttt
    38881 tcgatcggat ttagcttttc tttcggagct gaatgatcga tttgacgcgt caattccttg
    38941 taagcaggag ccaatccctg atccatgatt atttctgcat atctatcgag taaaatgaaa
    39001 tacttaatgg aagggatttg ctgttttaat ttttctgcat tggctactgg ctgttttttc
    39061 aatttgatac gaagccgagt cgcacatgga aggacagaat caatattgtc ttttcctccc
    39121 aactgttcaa tcagcatatg aatctgctcg gacagtacca tctgttcagg attcattgtc
    39181 gtttccatta gaagaccact ccactcacta atataatatt ggcgtaaggt gtcatttcgc
    39241 ctgtacgaat aaatgcatga cagttattca actctttttt catttcttca tgtgggataa
    39301 agctaatcgg tgtatctggt aacaataatt tgattttttc caatatttcc gggttcattg
    39361 tctttatttc ttccgccaat tcaatcgatt gtacctcaag ttcttctaaa acgttttcta
    39421 aaacagataa aaaacttgga agaccgtttg agacagccaa atctattttt tcagtaccaa
    39481 aggggacagg catgcctgca tccccgatac ttaatttatc aaagtgcccc atttgtgcga
    39541 ttactcgtga aatatctgaa ttgattactt ttcctttttt catgtttgtt tcttcctctc
    39601 attgttaatt ttagactgct gattgttcta aagcttcgcg gtaagggata gaaggttgcg
    39661 caccataaga ctgcacagca acagaagagg ctagatttcc ataaaggatc gcttctttca
    39721 agttactaag gtctggctga agtgtgctgc ttaatgcgcc aataaatgta tcacctgcgg
    39781 ctgttgtgtc aacggcatcc actttaaaag cagggacgat accgtgttct ttttctgtat
    39841 gatagaaagc gccggcactg cctagtgtaa tgattactgt tccaatacct aattcatgga
    39901 gtttttcggc tgctgcgacc aaactgttat gatctgtcac tctgattcct gtaatgattt
    39961 ctgtttctgt ttcatttggt ataatgatat ctgtcttttc aagtagttca gtaggaatcg
    40021 tttcacgagc tggtgcagga ttcaggatcg ttgtttttcc tgcgtctttt gcaatagaaa
    40081 aagctaggat cgtcgcatct aagctacttt caaactgagc aataataaag tcactgtttg
    40141 taatcagttg cttgtttttt aggacttctt gttcatggaa agcattattt gcccctgcat
    40201 ggatcaagat actattttct cctgctgcgt ctaccataat cattgcttgt ccggttgtgg
    40261 cgccttggat ttcagctatc ccagatgtat cgatgttttc ttttgtcagt agatctaata
    40321 actgttgtcc ttcgctgtca gccccaacgc ctccaataaa agatgtccgt gcgcctgaac
    40381 gtttggctgc cactgcttgg ttggcacctt tacctccgcc agcgtgaaaa atctcatgcg
    40441 catgcatcgt ttcgcctggc ttgggcatgt tggttaaacg aagggtagtg tccatattaa
    40501 tacttccaat tactgtaacc gtgttcattt tacaaacctc caactatctc atttattttg
    40561 atgggaaaat tattttttaa aacgttttag taaaacgttt taatagttcg ataaaaatat
    40621 agcacacctt gaaagctatt tcaagggtgc agttgaaaat cttttttcta atcgtacagg
    40681 taatcttttt tcttcccatt ctttttctgg gaattggatc cgatctaaca ataacttggc
    40741 agatgtttgt cccaattcga agattggttg tgcgatcgtt gtcagtttcg gcttgatata
    40801 ctcgcacata tctatattat catagccaat aatgctgtag tcttcaggga tgctttttcc
    40861 agcttcctct aatccgcgat ataatccaaa agctaattca tcatttaaag caaacacacc
    40921 agttgcagca gaatcaagaa gttctgctgt gatctggtag ccaccttgtt ttgagaactg
    40981 tgtaggtaag aggatcagtt ggtcgtgagg gatttggtaa acatcaagag cagatttaaa
    41041 cccctcaatc cgagcgtgga cattttccgg tggattttca ggatagacaa gggcgatcgt
    41101 ttgatgtcct agactaagca agtgcatgcc cgctaggtaa ccaccgcgaa aatcgtctgt
    41161 tcgtacggca tcactaaatc cttcagattt tttctgatcc aaaacaatga agggccggcc
    41221 ttgttttttc aaattttcgt tgattgcatc tgtagagaca gcagaagtcg cgataatgaa
    41281 tccatccaca cctcgacgtg tgagctcggc tagatattcg atttcttttt gatgatcaga
    41341 atcagcatta cataatatcg tgacgaaatt ctgcttatac aagatatcct caattcctcg
    41401 catcagtgta ctgaagaatg gattcgtgat atcaggaacg agtacaccaa ttgttttcgt
    41461 ttccttggta atcatttgtc tagcaaaata gtcaggctga tagcctaatt cttctttggc
    41521 agctaaaact ttctgaacag ttttcggact gaatttctcc gtatttccat ttaatatcaa
    41581 ggagacagtt gtgattgaaa cgccggaaag tttagcgact tctttgattg tgatcttttt
    41641 tttcaccatg ttcatactcc tatctaaatc ctgtggttat tttcccatga aatcaaaaag
    41701 aagtaaatga aaatcacatg tgccagtact tatttgcttt gttggatagc ttcgattatt
    41761 tgtttcgttg agcgtactcg tgcagttttc ggaaatatct tttttatgaa caattgatgg
    41821 gattctttgt cacgatcact gcaagcatct tcagcaagca cgatatggta cccgtgttga
    41881 aaggcatcta atgcagtagc atatacaccg tttgttgtcg ctacaccgct taaaatgata
    41941 gtttcgattc cacgccggcg aagttgaaga tcaagagaag taccaaaaaa agcacttgga
    42001 ttgtattttg tgatagtcaa catattatct gtatctttca aagaatcctt caaaagtaaa
    42061 tcagtgaatt ctggaggtac agaagcagtc attccaccca tatcagtttg ttgactcaaa
    42121 taagggaaag tggagatgtc tacgttcact aaaactttca aggcagcggt acgtttcaat
    42181 gttcttacta acaaatcatt gtttgtcaga atagatgctg cagtgtgtgg agagagtgta
    42241 ccgttattta cgatctcttt ttgcatgtcg atcgcgacaa aagcagtttt tgataaatca
    42301 aaaatcatat tttcctttaa atcattcatg atgttccttc tttcaaataa caatgttttt
    42361 tatgtaagta aatcataaat cgtttatttg tcaactaaat taaaggattc ctttctttag
    42421 tctagagaaa ttcagtggta aaataaataa gtgtgagaaa acgcattcga tgcatagata
    42481 tagcgagaaa tatcgtgaaa atggaggaat cagagtgaag tatgtcgaag taaaaaatga
    42541 atacaaacga tacaaaatgg gagaaacgac cattacagca aatgacgata tttctttttc
    42601 aattgaaaaa ggcgaattag tgattatact tggtccaagt ggagcaggaa agtcaacgat
    42661 tcttaatatt ttaggtggga tggatacacc tgatgaaggt cagatcatta ttgatgacac
    42721 agatattgcg caattttcag ataaacagct tactgcttac cgacgaacag atgtagggtt
    42781 cgttttccaa ttctacaatc tcgttcctaa tctaacagca aaagaaaatg tggaattggc
    42841 aacggaagtt tctcctgatg cgcttgatcc tgtagaggta ttgaggcaag tgggcctagc
    42901 tcatcgattg aataactttc cgtcccaatt atcaggaggc gaacagcaga gggtttcaat
    42961 tgctcgtgca ctagctaaaa atcccaaact tcttttatgt gatgaaccga caggcgcgct
    43021 ggattttgaa acgggcaagc aagtattgaa attattgcaa aacgctagca gacaacacgg
    43081 caataccgtt ttgatcatta cacataattc tgctttagcg cccatcgctg atagagtcat
    43141 tcacattaat gatgctaaag taaggtctgt tgaattaaat gatcatcctt catccattga
    43201 tgagattgta tggtaggggg attttatgaa aacaagaaca tatcttaaag caggttttcg
    43261 cgaaattcgg caatctaaag gacgattcat tgcaatcatc cttattgtca tgctaggaac
    43321 acttttattt gtaggtgtca aaactgctgg acctgtcatg caaaaaacga tggatcacta
    43381 tgttggtaca gcaggacttt ctgatttgca aatcgtttct actggtggct tgacacaaaa
    43441 agatattgct caagcagaaa agatcccaga tgcaaaagta gaaacaggaa aacagatcta
    43501 ttattccaat actggcaaaa acgaagtgat tcaaatattt tcttataaca aaaataacaa
    43561 gcagaacaag ctacaattaa ctgatggaaa actcccggaa aaaccaaacc agcttgtatt
    43621 ggatgaaaaa gcggaagatg aagggtataa aatcggtgat atttatcaga ttgattcaga
    43681 tgaattgaaa gaaaaagagt ataccatcac gggctttgtc cgttctccat tgttcatcaa
    43741 caatttggag agaggatacg cgaacgtcgg aaatggtagc gtcgattatt ttgtctattt
    43801 acctgaatcc gtttttaaaa cagatattta ttctgtctta tatcttgatt ttacaaacgt
    43861 aaagaatttg aatacgtaca gcaaagctta caaagataaa atggagcaaa atcaagaaaa
    43921 ggcagaaaag tatttacaag atcgacccga agaacgctta gaagagctga aaaaatcggc
    43981 caacgaagaa ttagaaccgg cacagcaaaa aatagcggat ggcaaagcac aaacaacgca
    44041 agcaagaaca cggttagaag atgctaaaaa acagttagcg caacagtcag caactatcca
    44101 gcagcttcct gaagcgcaac gaacaatcgc tgttcaaaca ttatctcaac aagaagctca
    44161 acttgctgaa caagaaaggc aattagttga gaaagaacgt gaactggcct ctgcagagga
    44221 agaattgaat cgaaatgcag aaaaaattga aaatttaact aaaccatcat acctatacaa
    44281 tgaacgttca gaaaatgtcg gttttcaaga atttggtgat ctgtctgatc gtatcgctgc
    44341 gattgccaat gtttttcctg ttttcttttt ctttattgcc gcattgatca cctttacgac
    44401 aatgacacgt atggtagaag aaaaccgcag ggaaatcggg acattaaagg cgttaggtta
    44461 tacgaaagtt gaaatcgcaa aaaaatatgc aatctatgca agtctagcct cagggatagg
    44521 tattgtttta ggaacgatat taggaacgaa tcttttgcca aggatcattt ttgagctgtc
    44581 taacgagcgg tatgatattg ggagtgcgct tattttttat gactggccgc cgattttaca
    44641 agcagctgcc gcattcttca ttgccgcatt tggcgctgca atgatcgttt tattcaaaga
    44701 tttaagagaa cggcctgctg cattgcttca accgaaagca ccgaagccag ggaaacgcat
    44761 ttttcttgaa tacatcactc cgctgtggtc acgccttagc tttaatcaaa aaatcagtta
    44821 ccgaaatctc ttccgatata agtcaagaat gcttatggcg attatcggaa ttgctggatg
    44881 tgccgggctg atggttgcag gcgtgggatt gaaagattct ctaagttctg tttcagccaa
    44941 acaatttgga acgatcattg actatcaagc aatcgttaca ttggattcat ccacggaaga
    45001 tagtcgtcaa aaagcagcgg atatgctgca gaagcaagaa aaagtaaatg agatcctagc
    45061 tattcatact caaaccgtag aattgagaaa aaaaggacat gcttcgcaaa gtctgacagt
    45121 gatcgtacca agtaaaacag ctgatttgga tcgttttatc catttaactg atcttgaaga
    45181 gaacccacgc tccttgccta aaacaggaat cgctattacg caaaaagtag cagaactatt
    45241 tgacttatct acaggagatc atatttcgct ttatgatgaa gatcaaaaag aatggaaagt
    45301 cgaagtaaaa gaaatcgtga aaaactatct aggtagcttt atttatatgt cccctgaata
    45361 ttatgcttca gttactgatc aatcgatgat ggataatgca tttttgctgc gtacagataa
    45421 gatgacaaca aaagaagaag aaatgctttc cgagaaactg cttgccacag acgctgtgac
    45481 aaatacgtct ttcatgtcca aacaaatcga aacgcaagaa gaatcaatgg cgaacttaga
    45541 ctcggttgtc ttaatctttg tcgtattatc aggactgctg gcttttatcg tcttgtataa
    45601 tctaactaat atcaatattt ccgagcgtgt acgcgaatta tctacaataa aagtccttgg
    45661 attttttgat aaagaagtca cgatgtacat tgttcgagaa aatatcatct ttactctttt
    45721 aggaatcatc ggaggatttg gtgtaggata tatattgact gacttcatct tgcaacaagc
    45781 gtctatggag aatgtcatct ttccattagt gatcacatgg tgggcttatg tcttatcagc
    45841 aggattaact atcgtattta cggttattgt gatgattgtt acccatttca aattaaagca
    45901 tatcgatatg attgatgcgt tgaaatcgaa tgaataaaaa cagaggttgt gaaatcagcg
    45961 gtttgacctg agtattagag aaacaaacgg caaaatagtt tttcatattt tgccgttttt
    46021 tgagctaata ccgaaggaca gctgattgaa caccatgaat cagaggttgt aaaaaagccg
    46081 tcctgcatcg agcaaaaaga atactcaaat aaaattagct cctcatgttt tacatttgag
    46141 aagactgatt gccgtagata aaagcttttg aacaccattt atagaagaaa caagaatatt
    46201 ttgctatttt aaacaagagg atagacaaat acatgtctga tctcttgttt tttattgggc
    46261 aacagacctc ttagaatgta taaagataaa ttttacaaac gctttcggtt cagttataat
    46321 aataaatgat aacttgtata tataagttgt ggtaggtaat aaatcagttg aatggaggta
    46381 aatatccatg acatttaaag acaaagtcat ttaccaaatc tatcccaaat cttacaaaga
    46441 cagtaatggt gatggaattg gagatttaaa aggaatcaaa gaaaaactcc cgtatctgaa
    46501 tgatctaggc atcgacatga tttggctaaa ccctatttat ccaagccctc aaaaagacaa
    46561 cggttatgat atctctgatt acacagccat cgatcctatc tttggtacga tggaggaatt
    46621 cgaacagcta atagccgaag caaagaaata ccggatagaa atcatgctcg atatggtgct
    46681 gaatcacgtt tcgacagaac atgaatggtt caaaaaagca ctagcagggg accagcatta
    46741 tcaggacttc ttcattttaa gagaagagcc aacggattgg gtatcaaaat ttggtggaag
    46801 tgcttggtcg ccatttggag atacggggaa atattatttg cacttatatg atcggacaca
    46861 agctgacttg aactggcgga accaagcagt aagagaggaa ctattcaaag ttgttcgttt
    46921 ttggatggaa aaaggggtaa aaggattccg tttcgatgtt atcaatgtta tcggtaaaga
    46981 cgaacaattg aaaaataata aagaaaatga tggcaaacct gaatatacgg acaaaccaat
    47041 cacccatacg tatctaaaag aattgaacca agcaacgttt gggacggatt ccactatcat
    47101 cacagtcggt gaaatgagct ctacgacgat cgaaaattgt atcttgtata cagaacctag
    47161 tagagaagaa ttatcaatgg tctttaattt ccatcatttg aaagtcgatt atgaaaatgg
    47221 gaataaatgg tctactcctc catttgattt tgcagaatta aagcgactat ttcatacatg
    47281 gggagaaaag atgagtgagg gtaacggctg gaatgctcta ttttttaata atcatgatca
    47341 gccacgtgcg ctcaatcgat ttgtagctat cgatcagtat cgaaaacaag gagcagagat
    47401 gctagcaacc atgatccatt tgaaccgtgg gacgccctat atttatatgg gagaagaaat
    47461 cggcatgatc gacccagact attcatcgat ttccgactat attgacgtag aaagtttgaa
    47521 tgcttataag ttactccttg aacaaggatt cacagaaaaa gaagcgtttc gtcggatcaa
    47581 agcaaaatct cgcgataact ctcgaacacc catgcaatgg tcagacacag aacaagcagg
    47641 atttactaca gggaaacctt ggttaaaagt tggaggcaag ctagaagaga ttaatgtaga
    47701 aaaagaacta tcttctgaag attctatttt gtcctattac aagaaattga ttcggctgag
    47761 aaaaacatat ccaatcgttg cacaaggaga ttatcatgct tatgaagcaa atcatccgga
    47821 agtttatggc tttcttcgcc aatttgaagg acagcagctg cttgtattaa caaatttcta
    47881 tgataaagat acaaggatcg ccatcccaga aggattcaca gaagccaata ttcttatcga
    47941 taattattcc gatactgttc ttcatcaaag tctggagcta aaaccatacc aggcaattgc
    48001 tttgctgaaa aattaaaaca aaaacacaaa acgtcgttcc tacgagctat tctcgtaaga
    48061 tcgacgtttt gtgttttatt taaaataatc ttcttcagaa aatgaagagt tttcatactc
    48121 tcctttatcg ccataaaatt tattgtagcg ttgtttgaat ttacggaaaa taaaagtaac
    48181 gagtaatttg actaacgtat agataggaat accaaaaata acgcccacaa ttcccattag
    48241 atcacccatg acaagtaaaa cgattaagat cgtaatcgga tggatctgta aacgatcacc
    48301 cattactcga ggaatcacca agtcaccgtg gagaagttgt acggcaaacc agacaatgac
    48361 aaatttgaca gccataaaag tagagtcttg gaatgcaatg atcaaacctg gaaagaaggc
    48421 gataaacggc ccaatatatg gaatgataca taacaaccca gcagaaatag ctaaaacact
    48481 agcaaattct aatccgatca gtaagaaaca aacccagtag actgctccaa ggataaatga
    48541 agcaatgatt tgccccttta gaaatgaacc caattgttga ttagcaatct ttgtcaaatt
    48601 tttaaaatct gtacgaaatg ctggtgggac aatcgctaaa actgactgat aaaatttttg
    48661 aggatctctc aaaaggaaaa aagcgacaat tggaccagta aataaagtaa taaaaatagc
    48721 tgtaacagtt gaaaaaatgt tgctgatccg ttgtgcgcca ctttcccaat aatttccaat
    48781 aaatccagct atatcagacg tcacgtcgtc aatagaagcg aaaaattgat taaatgaatc
    48841 ggcaaaaggt gttttctcca aaaattgtcg gagactctct tgaaaatctt gaaacaaatc
    48901 tggcaaacta ctgatcaatg tctttgcctg ttcttcaaga aaaggaataa gccatatgct
    48961 tccaagcccc aataaaaata aaataatcac ataaacgata atgactgaca agttcctcga
    49021 aaggtgcttg ctcaaacgtt tgatgactgg ttccaaaaga taatacaaga tcagtgcaaa
    49081 caataatggg ggcaagatag tagcgataat gattccgaca ggcttgaata taaacgagat
    49141 ctgatggaaa aaataaatga tcagcgcaag taagataact attcctaaaa catagtaaga
    49201 ggtttttcct cctaaaaagc ggatcaaacg attcgttgta tctgtttctt ttttcactct
    49261 ttttcctcct ttatctataa cttcattata gacaaaagcg ggtataaaag gaaaatgata
    49321 gaggttgatt gcaaaaatat cgtcaatcat tcatataaac tttctcatca attaaaataa
    49381 gaaaacgctt gacagttctt ttaaccatat gataaattaa atcctgttgc taaaacgtaa
    49441 ctattacggt ttgcgaaaag cgaatcggaa cattcaaatg aaagaagatg agaagaggag
    49501 tgtcataaat gaaacgattg ataggtatat tggccatgct ggttttagca ggtatgttaa
    49561 ctgcttgcgg ggcaagtggg aaagccgagg acagtaaaga aaaattgagc gtgatgacga
    49621 ccttttatcc catgtatgat tttacaaaag caatcgtcgg ggatgaagga gaagtagagt
    49681 tattgattcc agcagggact gattcccacg attatgaacc ttctgctaaa gacatggcaa
    49741 agatccaaga tacagatatc tttgtttata atgatgaaaa catggaaaca tgggtccccg
    49801 caatccaaaa aacattacaa gaaggcaatg tccatacgat aaaagcgaca gaaggtatgc
    49861 ttttacttcc gggaagtgaa gaaggacatg atcatgacca tgaacacggg gaagaaggcc
    49921 atacacatga gctagaccca catgtatggc tagctccaag tttagcaatc aagcaggtag
    49981 caaacatacg tgatcagctg attgaagctt acccagaaaa acaggaagta tggacaaaaa
    50041 atgcagcagc ctataccgag aaacttcaag cactccatca gctttatcaa gaaaccttta
    50101 agcaagcaaa acaaagatct tttgtcacgc aacatgctgc attcaactat ctagcgttag
    50161 aatatggatt gaatcaagta tcgattgctg gtctttcttc tagtgaagaa ccgtctgcgg
    50221 cgagaattgc tgaactgaaa cactttgtta aagaacatgg aatcaactat atttattttg
    50281 aagaaaacgc caaagatagt attgccagaa cgttagcaaa cgaagcaggt gtatctttag
    50341 aagtattgaa tcctttggaa ggattgacga atgaacaaat agaaaatgga gaaaactatc
    50401 tttctatcat ggaagcaaac ctcgaagcgt tgaaaaaaac gacagagaca gaaaacccgc
    50461 tagaagatac tttgaccccc aaaaaagaaa agagcgtcta caacggttat tttgaagacg
    50521 aggatgtaca agatcgctct ctttcagatt gggcaggaac ttggcaaaca gtagatacgt
    50581 atgtagaaga tgggactttt gatcctgttt ttgaatataa agaaaaattg aatcaggata
    50641 agacagcaca agaatataaa gaatattata cgaagggcta tcaaacagat attgcttcaa
    50701 tcaagatcag tgagaaagag atggcattta cgtttaagaa tggaacaacg aaacgttcaa
    50761 attatcgtta tgtagggaaa gaaatcttga cctacgaggc aggaaaccgt ggcgtaaggt
    50821 atctattcga agcagaagat aaggaaagtg gcgcttttag atatattcaa ttcagtgatc
    50881 acacgattgc accggctgac tccgaacatt ttcatttata tgcaggaaat gaaagtcaag
    50941 aagtattgct gaaagaaatg gagaattggc caacatttta tccagataca atggatggac
    51001 gtgaaattgc acaggaaatg cttttgcact gataggcaaa aaagaaccga agggtttgga
    51061 tcggctggtc atttagtcgg tccaaactat tcggttcttt ttatgaaatc aatttcttta
    51121 atccacggat agaggctggg atggtctcgc cgtttgccaa cgtgacttct ctggtttttt
    51181 tattaatcga ccgcacattt tgtgtattga taacatagga tttatgacaa cggataaaat
    51241 tccgatgcat tttctcgatt tctttgatct tctcatagaa ttgtaattgg gcatgagtca
    51301 aatggacttc cagtttatgt ggcgctccta gcgaagtaga aaaatattgg atgtctttga
    51361 tcggtaaaaa tttgatctca tcattaaact ctattttgaa gatatctttt tggatttggg
    51421 acatttgaaa atcttcgaaa attttgctca ggatatgctc gacattttct tttaaatcat
    51481 aaagatcttc tttgacgata taatcagtcg gttcgatgtt gctttttaag attttcatcg
    51541 acatttccgt atgggaagtc acatagatta tttttcctaa aggatctaat tcacggatca
    51601 atttaccgat cgctacacca tccatttcac tgtcttctag ttcgatatct aaaaagtaaa
    51661 taccaaaaga agaagttctg atcttggctg cattgagaag ttcttccgga tcagaggtag
    51721 aaatatggac ataactgtct aagttttcaa tcatgattct atttttgatc accgttgata
    51781 aaacttctaa ttgttttgga tcgtcttcac aaatataaat gggaatcata tcattactcc
    51841 tttattttat ttgtatataa atcgtttgag aaaaacgctg gttttcaatt gatgtttcaa
    51901 ggaaaagata aggattggtc tttgtcaatt catccaagat aaacaagcca aggccttcgt
    51961 tttttggatt tgtttttgtt gagaaccctt tttgtttcaa ctggtttaac ggtaatgtat
    52021 ttgttgttgt attgatcaca cgaatcatca cggaatgatt cttcttgagt atggtcaatt
    52081 cgattttttt cttctttgca gcactagctc cttcaatcgc attgtccaag ataataccaa
    52141 gcatacgtac aaggtttacc gtatgtttcg tatccagttc gattggctca ggaaaatgaa
    52201 gctggacgat caaccctttt tcctgtgcca tcatgagttt taatgacagt aaactacgga
    52261 tctcaggaac tttcatatta tccaacatac ttagattggc cggaaaaagg ttcatcatct
    52321 ggcgtgtagg taatatcgtt tgatcataat aagaccgtaa tccttctaag tcgccttctg
    52381 aaatatagct gtgaagagaa aaaagcagat tttggtaatc atgtttgaat tctctcaagt
    52441 cttgatagtt tttttctaga tctttgatat aatcctgttg catcctgact tgttgctcta
    52501 ataactgatc agctaatttc ttattagaga ttttccaata ataaaccaag atgatcaggc
    52561 caacacacag atatagaatg aaaaaactcg tattcaaagc taagacatca ggtgtttcac
    52621 cagaaaaacg ggtataaaaa ataatgatat agtatgtaaa taatatcaat gtcccgatcg
    52681 taccgatcat acggcggtcc acagtgtgaa accaagtctc taacgatttt tttaaacaaa
    52741 aagctaataa agcagataaa aaagcggata agacaatgtg aaaatactgc aatgaatctc
    52801 ctggttcaat aaccgaagat ttcaaaacaa aaaaatcgcc gatgcttgtt aagtgatctc
    52861 ctaaaagcga cgtcaataac ccaaaaagaa agcgcgtaaa ccaattgttg acgcgaagtc
    52921 gaaaagaaga aaaaagtaag catcagcatt cctatagcaa ttagagcagt aaaaatccct
    52981 aaaatcgaat agacccatcc aatcaaaata caagaaatca gaatgactcc cgcaaacaga
    53041 tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa ttgtgtaaat caaatattgc
    53101 aacgctatgg accataaaaa cataaagtac agctcccatt gatttttacc aaattatagc
    53161 acaattggcg tattattttt ttaatttttc agggattttg aattcagaac tcataccaaa
    53221 gcagcagcca gatacaaaaa agtcagataa gttagttaat tgtttaatga ttgttttttt
    53281 catgaaagat tcctccaaaa ttcatcaagt agtttgtaat aggaagcaac gtagctattt
    53341 ctaaaacaat cccaagtagc aaataggtag aaaagtaaga agctgagaaa aataacgtaa
    53401 tgagagaaaa aattagaaac atgctgatcg ccttctttaa taatggggga cgtttctcgg
    53461 gttcaatggg attgattgct gtacctcttg atccatagcg aagcagtaac aaaaagcagc
    53521 tgattaacaa aaagaccatc attgggaagg agatagtcaa ttgcagatgt ttcatcacca
    53581 agggaacgcc ccataaataa agtagtgtcc acagcagaca aacgaattca gagtcggaat
    53641 gcaatccata agcatatctg cggatgcaga tataagaaag aaaggtgatt cctgtttccg
    53701 cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc tagttttcca agattattca
    53761 ataaaacttc taagacaaat tttacttgga catacaccat atcttcttcc gatttgtctc
    53821 cgctgaataa ggcttctaat aatttgttca caactttatc tttcatctca tacgtacctc
    53881 caccacaaat tttatgctaa aaaaaataaa aaacgtcttt tgtttcataa aaagtggttt
    53941 tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt tttccaagaa ccttaccaaa
    54001 aaaagtgaca gttttgatac gaaaagtact ttttatgaca agtttgtgct ttttcgagtg
    54061 atttatgtta aaaaattact gaccttataa aaggtgatgg agggattctt atggaaaatg
    54121 aacaattgtt tgaagtcggc gaaacatatg catttaaatc tgatttattt gaatcaccaa
    54181 taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat gatacgtgtc atgaaatgcg
    54241 cagtagaaga caatgatacc gctcataatc tgcactggca gttagttgta cgcaaatcaa
    54301 gtacaataga agaataaaaa cagataaaat gttgagagca taaagaggtt gtgagtctcc
    54361 cgtttagctc tgagttacaa ggaataatgt taaaaaacag cttttcatgt tttatgaata
    54421 ttgccttgtg accgaggtgt tggcaggaga atactgttta tttgaaaaaa gggctgcgac
    54481 aagacttttg tcacagcctt tattttcatg aataggagtt acttttggct acttttagag
    54541 aagtagcttt taaggagcag ataaagtcaa ggcttttatt tctataaatg aagtagtaaa
    54601 atgaagaaca gattttatta caggaaggat gccgataatt gtgatcattg ttgcaggaac
    54661 gatcggtgca gggaaaagca cactaactga aatgcttgcc caagatttag aaacaaaacc
    54721 tttttatgag aacgtggaag ataatgaagt attgccgcta ttttattcta atccagaaaa
    54781 gtatactttt ttattacaga ttttcttttt gaacaaacgc tttttggcaa tcaaagatgc
    54841 tttcagccat gatgacaacg tactagaccg ctccatctac gaagatagta tgctgtttca
    54901 tttgaatgca gatttagggc gtgtttctga agtagaagta aagcagtatg aaggtctttt
    54961 agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa aagaaaccag atctactggt
    55021 ttatattcgt gtatcctttg aaactatgtt agcgagaatc aaaaaaagag gccgggaata
    55081 tgaacagcta gaacaagatc ctgaactcta ttcctactat aaagaactca atcgacgata
    55141 cgaagagtgg tatgaacagt ttgatatatg tcccaaaata gtgatcgatg gggacaaata
    55201 tgatttcgta gccgatccgg catgtggaga acaaatcgta caagaaatca agatgcgggc
    55261 aaagaaaatg acagaagagg cagatgccca gtcgatccgc tacactacaa ataacccgaa
    55321 agcaaaggga atctttacaa ctaaataata taaaaactaa gcaaattgct cactcgcaga
    55381 tatttctctg tgagcagcat cttgcttagt ttttttgatt cattgagatc aaatagtgaa
    55441 ccgattcgta tgataaaatt gccataaatg aaatgtctga gtgagggcca tgatcaaaaa
    55501 taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga tgatgacaga aaacaaggac
    55561 aatagctcct gcgataaagg caagaacgac acttagatag tggaaaaatc gatcaaaatc
    55621 tgctttttct ttagaaatac aataatcata taagttcgtt gctacccctt ttaaatttcc
    55681 agttgtaaat aaattagtgt aggcacgccc gttgatttta tcaaaagcta cccactggat
    55741 cgatgcagta aaagcaatca aaatcgttac taatgcggga tgctcatgga cgggtgtcaa
    55801 actaacaaag aaaaaaatca ctgcttcata atatagaaca aacaagcgcc agaaatgttt
    55861 ttcttttttt ctaaaataat caattagata ctttgttaga agaatcccta agaaaaagaa
    55921 aagggtagac aatacttttt taccaacact tagccattct ccgtcaaatg cttggatgat
    55981 cgatagaatg atattgccag tttgagcaga ggcaaaagca ccatattgga tataagtata
    56041 actgtccatg gctcctccga taaatgtcag caaaagtcct attgtacgcc cctcgtgaaa
    56101 ggaagtctgc atttttttca tcacctttta tgaatatttt tgtcttcctt atcttagcat
    56161 aaacgattga aaaaaaggaa aaattttcag aaaactaaaa tcatatcgct tcccattttc
    56221 atttttagaa aggtctgtta gtataaagaa aaaatagaca acgaaacttt aaataaccaa
    56281 cttgaaataa acaaattcaa agaagaaaga tgggaaggaa atcaatcagt tgaatgaaaa
    56341 agaaattctc cgattacaaa aagaattgaa acgctgggag aacaagtata aaaagcttca
    56401 acaagtcttt cggctacaag aagaaatcat tgctacctat gaagcggctt atggcgaagc
    56461 acccggactt attgaggaag aagtacaagt aaaagaaagt gaaggaaaca gctctttctt
    56521 taaaatgatg gagaaacgtt cctataatcg cttcaatcaa gaacaaaaag cagctatcac
    56581 atatgatatg aagaaacatc ttcgaatcat agcaggagct gggagcggca aaacgcaaac
    56641 aatctgtgca aaagctgttt atctgatgac gcaaaaacaa gtagacgaag aacgcatact
    56701 aatgatcact tttacaagga acgctgcaaa tgaactaaaa aaacgagtag ataatttcag
    56761 ccagagaaaa accgctgtcc atatcggaac attccatagt atctttttta ggatatacaa
    56821 tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga atacaaggag acttttcaaa
    56881 ggatactgca caaaaagtga atgctgtttt gcaacagctg atacgtaaat ataatcttta
    56941 tatatttgat caatatggag aaaaaacgat tgcttctcgg ttagactatt ggcagaatat
    57001 gaacctttca gtagaagaaa tggttcagtt agttaaagaa aaatatgatt ccattgataa
    57061 gaatgcacga cagccgatat cagaacgact gtatggattg ctcacggaac tccaagaaca
    57121 aaaaagaagc cagcaattgc tagagttcaa tgatgtactc caaaatctga aaagcgcttt
    57181 ggagaatgag gagatccagc attatatcgg ccaaaaatat gattatctat tcattgatga
    57241 atttcaagat actaatccac ttcaatggga aatcgtgaaa cggatgacaa aggaaaacag
    57301 gatcaagttg attgttgttg gagatgatga tcagagtatc tatggattca gaggatcaga
    57361 gccagcatac atcaagaact ttgaaaaaga atatcctacc aaaactcttt tcttattaac
    57421 taattatcgt tctcgggcag ctattgtcca aggagcaaat agattgatca gctataataa
    57481 aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa gaagcaggtg ttatggaagc
    57541 ctatttgttt tctgatacta aagcagaaag tcagtggcta atcaaacaaa ttcagacatt
    57601 tattcaaaaa aatgggaaat ataaggaatc aatcatatta taccgatcag cttcacaaac
    57661 gcagcagctt gtccaatctc tgctgaaaac gaatattcct tttgtactgg aagcagatag
    57721 tccatatgaa ggtatctttg gtatcaagtc tttccaacat ttctatcaaa aaattgttca
    57781 ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc taccagcaat tgctccggca
    57841 gctgatggca gactgctacc tgaaaaaaag tgaatgcgat caatactttt ctcctaaata
    57901 tcaaaatata tccgttgcag attatatttt gtcgataagc cccaatttaa aatcgaaatc
    57961 tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca gaccagcatc aagaaatcta
    58021 ttcgcttgtt caaagctatc ttcatctgcc acgtatagct aaggaaatcg accagagtga
    58081 tcaagaatgg atacgagaag aagtaaagca gcatgctact ttcagaagac tccaagcctt
    58141 atcgcaagaa acccaagcga cagctaaaga attaaaaacc cgcttgattg cttacagaca
    58201 aggaaaactt gatgcactat gtatccagag cattcacaaa agtaaaggtc ttagttaccg
    58261 aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct tataacggag cgacagaaaa
    58321 gaaagtaatc gactttaatg aaataaaagc agaaccagta acgacgatag aagaagaacg
    58381 aagactattt tatgttgcga tgactagagc aagaaatcgt ctgtatctat gtgtacctca
    58441 aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc aaagaaaccg ggagtcgtgt
    58501 gaaaaaagga aagtgataga agagagggtg tgacagaagt ggtcagtttc aagaaagaag
    58561 aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat ttcagcttat tttcgaagaa
    58621 gctatttctg gaacacagtt tattcggaaa agggaggtgt gacaagaatt ttgtcacacc
    58681 tcccttattt attttgaaaa tgagaatact attatcaaaa tagagaatat atagaaaaat
    58741 tctcattatt caaacgtaaa taatcagtta ttttgatttt ttttatattt tattagctaa
    58801 taattggtat tttaaaagaa attactgtag aattggatta taattaaaaa ggagtgagaa
    58861 aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt
    58921 gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag
    58981 tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg
    59041 gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat
    59101 cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag
    59161 aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg
    59221 tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag
    59281 ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac
    59341 aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatctaa
    59401 gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg
    59461 cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac
    59521 aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg
    59581 atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat
    59641 agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac
    59701 ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg
    59761 ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga
    59821 catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat
    59881 agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa
    59941 ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa acttcaaaaa
    60001 agttcttgac aaataacaaa tgataagtta tgataaatga gttgctgtaa gatttcgaaa
    60061 aacgaaatca aaaaaacttt taaaaaagag ttgacaatta cttgtctctc tgatatgata
    60121 taaaagttgc tacggcagcc aaaaacgaat cggggaacac tcctgatgaa gtagaccttt
    60181 gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg tcttgattca attcaagaca
    60241 acaaaca
//
LOCUS       DACEKG010000005        57353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEKG010000005 DACEKG010000000
VERSION     DACEKG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.005"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(208..2100)
                     /locus_tag="IW664_000253"
     CDS             complement(208..2100)
                     /locus_tag="IW664_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847648.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2097..3824)
                     /locus_tag="IW664_000254"
     CDS             complement(2097..3824)
                     /locus_tag="IW664_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847649.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3840..4490)
                     /locus_tag="IW664_000255"
     CDS             complement(3840..4490)
                     /locus_tag="IW664_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0847650.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4694..4990
                     /locus_tag="IW664_000256"
                     /pseudo
     CDS             4694..4990
                     /locus_tag="IW664_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            5162..5782
                     /locus_tag="IW664_000257"
     CDS             5162..5782
                     /locus_tag="IW664_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0847651.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5874..7040)
                     /locus_tag="IW664_000258"
     CDS             complement(5874..7040)
                     /locus_tag="IW664_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0847652.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7052..7405)
                     /locus_tag="IW664_000259"
     CDS             complement(7052..7405)
                     /locus_tag="IW664_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0847653.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7533..8555
                     /locus_tag="IW664_000260"
     CDS             7533..8555
                     /locus_tag="IW664_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0847654.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8994..9428)
                     /locus_tag="IW664_000261"
     CDS             complement(8994..9428)
                     /locus_tag="IW664_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847655.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9538..11397)
                     /locus_tag="IW664_000262"
     CDS             complement(9538..11397)
                     /locus_tag="IW664_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0847656.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11515..12675)
                     /locus_tag="IW664_000263"
     CDS             complement(11515..12675)
                     /locus_tag="IW664_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0847657.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12982..14406)
                     /gene="araA"
                     /locus_tag="IW664_000264"
     CDS             complement(12982..14406)
                     /gene="araA"
                     /locus_tag="IW664_000264"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0847658.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14422..15120)
                     /locus_tag="IW664_000265"
     CDS             complement(14422..15120)
                     /locus_tag="IW664_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0847659.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15077..16738)
                     /locus_tag="IW664_000266"
     CDS             complement(15077..16738)
                     /locus_tag="IW664_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0847660.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16956..18356
                     /locus_tag="IW664_000267"
     CDS             16956..18356
                     /locus_tag="IW664_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0847661.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18608..19687)
                     /locus_tag="IW664_000268"
     CDS             complement(18608..19687)
                     /locus_tag="IW664_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0847662.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19773..20732)
                     /locus_tag="IW664_000269"
     CDS             complement(19773..20732)
                     /locus_tag="IW664_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0847663.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20744..22267)
                     /locus_tag="IW664_000270"
     CDS             complement(20744..22267)
                     /locus_tag="IW664_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0847664.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22277..22840)
                     /locus_tag="IW664_000271"
     CDS             complement(22277..22840)
                     /locus_tag="IW664_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0847665.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22846..23709)
                     /locus_tag="IW664_000272"
     CDS             complement(22846..23709)
                     /locus_tag="IW664_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0847666.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23723..24619)
                     /locus_tag="IW664_000273"
     CDS             complement(23723..24619)
                     /locus_tag="IW664_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0847667.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24794..26065)
                     /locus_tag="IW664_000274"
     CDS             complement(24794..26065)
                     /locus_tag="IW664_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0847668.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26055..27572)
                     /locus_tag="IW664_000275"
     CDS             complement(26055..27572)
                     /locus_tag="IW664_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0847669.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27770..28660
                     /locus_tag="IW664_000276"
     CDS             27770..28660
                     /locus_tag="IW664_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0847670.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28733..29557)
                     /gene="nadE"
                     /locus_tag="IW664_000277"
     CDS             complement(28733..29557)
                     /gene="nadE"
                     /locus_tag="IW664_000277"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0847671.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29569..31041)
                     /locus_tag="IW664_000278"
     CDS             complement(29569..31041)
                     /locus_tag="IW664_000278"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0847672.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31678..32040)
                     /locus_tag="IW664_000279"
     CDS             complement(31678..32040)
                     /locus_tag="IW664_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0847673.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32263..33015
                     /locus_tag="IW664_000280"
     CDS             32263..33015
                     /locus_tag="IW664_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0847674.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33155..34078)
                     /locus_tag="IW664_000281"
     CDS             complement(33155..34078)
                     /locus_tag="IW664_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0847675.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34093..34365)
                     /locus_tag="IW664_000282"
     CDS             complement(34093..34365)
                     /locus_tag="IW664_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847676.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34835..36460)
                     /gene="groL"
                     /locus_tag="IW664_000283"
     CDS             complement(34835..36460)
                     /gene="groL"
                     /locus_tag="IW664_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0847677.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(36511..36795)
                     /locus_tag="IW664_000284"
     CDS             complement(36511..36795)
                     /locus_tag="IW664_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0847678.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            37020..37682
                     /locus_tag="IW664_000285"
     CDS             37020..37682
                     /locus_tag="IW664_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0847679.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(37758..39050)
                     /locus_tag="IW664_000286"
     CDS             complement(37758..39050)
                     /locus_tag="IW664_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0847680.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            39220..39849
                     /locus_tag="IW664_000287"
     CDS             39220..39849
                     /locus_tag="IW664_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ0847681.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(39952..40761)
                     /locus_tag="IW664_000288"
     CDS             complement(39952..40761)
                     /locus_tag="IW664_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0847682.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(40816..41685)
                     /locus_tag="IW664_000289"
     CDS             complement(40816..41685)
                     /locus_tag="IW664_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847683.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(41686..43002)
                     /locus_tag="IW664_000290"
     CDS             complement(41686..43002)
                     /locus_tag="IW664_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847684.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(42999..44834)
                     /gene="walK"
                     /locus_tag="IW664_000291"
     CDS             complement(42999..44834)
                     /gene="walK"
                     /locus_tag="IW664_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0847685.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(44839..45543)
                     /locus_tag="IW664_000292"
     CDS             complement(44839..45543)
                     /locus_tag="IW664_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0847686.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(45733..46593)
                     /locus_tag="IW664_000293"
     CDS             complement(45733..46593)
                     /locus_tag="IW664_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0847687.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(46580..47149)
                     /locus_tag="IW664_000294"
     CDS             complement(46580..47149)
                     /locus_tag="IW664_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0847688.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            47530..47602
                     /locus_tag="IW664_000295"
     tRNA            47530..47602
                     /locus_tag="IW664_000295"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47563..47565,aa:Lys,seq:ctt)
     gene            complement(48098..48484)
                     /locus_tag="IW664_000296"
     CDS             complement(48098..48484)
                     /locus_tag="IW664_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0847689.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(48481..49002)
                     /locus_tag="IW664_000297"
     CDS             complement(48481..49002)
                     /locus_tag="IW664_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0847690.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(49004..50038)
                     /gene="sppA"
                     /locus_tag="IW664_000298"
     CDS             complement(49004..50038)
                     /gene="sppA"
                     /locus_tag="IW664_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0847691.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(50071..51486)
                     /locus_tag="IW664_000299"
     CDS             complement(50071..51486)
                     /locus_tag="IW664_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ0847692.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(51455..53608)
                     /locus_tag="IW664_000300"
     CDS             complement(51455..53608)
                     /locus_tag="IW664_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ0847693.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            53866..54102
                     /locus_tag="IW664_000301"
     CDS             53866..54102
                     /locus_tag="IW664_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0847694.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            54121..54336
                     /locus_tag="IW664_000302"
     CDS             54121..54336
                     /locus_tag="IW664_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0847695.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(55039..55728)
                     /locus_tag="IW664_000303"
     CDS             complement(55039..55728)
                     /locus_tag="IW664_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0847696.1"
                     /translation="MVLIKSMNYSGFKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDL
                     NHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNE
                     IVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDG
                     QYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQ
                     KIKEKLKESTV"
     gene            55852..57171
                     /locus_tag="IW664_000304"
     CDS             55852..57171
                     /locus_tag="IW664_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0847697.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT        16989 a        11787 c         9673 g        18904 t
ORIGIN      
        1 ttttgaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga
       61 tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata ccgaaggaca
      121 gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg tcacagcccc
      181 taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa actgactgtt
      241 ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc ctgtttctac
      301 gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag acaagcggtg
      361 agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt gaatcagtat
      421 ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat ctggatttgc
      481 taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat tgcttgcatc
      541 ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt ctacgtgagc
      601 agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat atttgatatt
      661 gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa actgtgcgcg
      721 caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac tacctccaga
      781 aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc
      841 tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca ttagcaagtt
      901 atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg tatctttttc
      961 ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt caaatacccg
     1021 ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt gagtgattgg
     1081 ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg tcatgttccc
     1141 atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat ttttaacgaa
     1201 attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag cggcatacag
     1261 attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga atgttttaac
     1321 aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta aacttttttg
     1381 ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga tcaagcttaa
     1441 agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca tcatcacgat
     1501 gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct gcagcgtgct
     1561 tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg tatgtgtatc
     1621 ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta attcataaac
     1681 tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata cagcagaaat
     1741 caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa
     1801 ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac ctttgaatat
     1861 ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca cggctgctat
     1921 tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc caaacaatcg
     1981 gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat ttctgccagg
     2041 tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg ttttactcat
     2101 gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc gtttgttgct
     2161 aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac aaggatcata
     2221 tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact ttctttcata
     2281 ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc tgagaaggaa
     2341 tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc cagacgctgt
     2401 ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa gccatcttcc
     2461 atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat catttcatct
     2521 gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa cagaactgct
     2581 ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc actaatgtcc
     2641 gttccattta tttggatcga accagattcg atgtcatata atcgagggat caaattgact
     2701 aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc cgacttggct
     2761 tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata attgacatga
     2821 ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt ttcagggtca
     2881 tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc ttgtgcacga
     2941 ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg catcgcataa
     3001 gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta gtgtccacca
     3061 aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg catcatcaat
     3121 gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc taaatcaaaa
     3181 cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc tgtcaatcct
     3241 tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa taatggaaca
     3301 gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac taagaagatt
     3361 tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa cgtgattggt
     3421 gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt cacatcattg
     3481 gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt tgtcagactc
     3541 tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga ttcacgagta
     3601 gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc gatcatcaca
     3661 aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc aataatattg
     3721 gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa aactgcagct
     3781 gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt cccttctttc
     3841 tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact gattgcttgc
     3901 tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat
     3961 acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt tactttcagc
     4021 ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc tttatgtgga
     4081 tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc acggtaatcc
     4141 atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat ctcataaatc
     4201 attttagaaa aataggattc gaatcctttg aatagatcac catcggattc tttcattagt
     4261 tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa ataaagatcc
     4321 tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa tttgacgata
     4381 ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc agcctcgatt
     4441 agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc
     4501 ctttctttca agatgacaca ttgtcatatg acgctgtgtc attataacac ctcctacctg
     4561 ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa aaagtgataa
     4621 gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac atactaaaca
     4681 aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat tgcctctcga
     4741 tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa accacttaag
     4801 ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa
     4861 gaaactttaa tatattctaa gctatacagg caggcgatca gaagcattag ccgatcaact
     4921 tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc ttcagtcgag
     4981 tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag
     5041 cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca atatgacttg
     5101 atagcctttt ctttttctca tagttataac aatcatagca ataaaaggag aaggtaagca
     5161 tatggcatct gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt
     5221 taaggcattg acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac
     5281 tgttttggaa ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg
     5341 ggaagcatta cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat
     5401 tgctcgtttc aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa
     5461 cgccttatgg aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt
     5521 agctggaaaa acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa
     5581 agcactgcac atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata
     5641 tgtaaaaggt attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg
     5701 aatcgattat gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc
     5761 tgctttaggt aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa
     5821 aatcttcaaa gcagcatttg taccttggat attttcccat ctgtctcttt ttgttattct
     5881 ttctgctttt tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa
     5941 acatgagtaa tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg
     6001 attccgaaca atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct
     6061 aactgattcc ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca
     6121 aaaacgatca gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc
     6181 ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc
     6241 cccaacgtga cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc
     6301 acagcaaaag ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca
     6361 ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt
     6421 gctaattgtt taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc
     6481 actaaaatag tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat
     6541 atatcttgga tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt
     6601 tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta
     6661 tctacatatt gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg
     6721 atcactacgc tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg
     6781 taaagccttg gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac
     6841 aagattcctg ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca
     6901 aacaaatatg aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt
     6961 aaggctgtga ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc
     7021 ttcttatttt ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac
     7081 caatagcagg cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg
     7141 gacaagcatc aaaaatcaat gatacgtgag ctactgtctc taaattccca ttggccatcg
     7201 gaccgacata atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca
     7261 aagaaaaagg ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg
     7321 ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa
     7381 tttctgtaat ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta
     7441 taacacattc tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac
     7501 tatttcatta caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct
     7561 atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat
     7621 tagccaacct ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag
     7681 ctggcaatct ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata
     7741 aggattttca ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg
     7801 ttgatgaagc acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct
     7861 accatatatt aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga
     7921 caggtcctca taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc
     7981 aacgattagt cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa
     8041 acggcaaatt gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc
     8101 ttgtctttca tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc
     8161 gcgtgctttc ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct
     8221 tgccagaaga attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca
     8281 agttccttta tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag
     8341 atggttccca cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt
     8401 tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga
     8461 cattatatcg ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg
     8521 taccagaatg tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc
     8581 agcaagacgt attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa
     8641 tcgaagataa agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact
     8701 actttgaaca atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag
     8761 aacatcagga caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc
     8821 cgagatggtt agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt
     8881 gttgttggtt ataggtatgt cattatcata aaggattttg atcactctat cataaaggta
     8941 atattacatt tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt
     9001 ttcaacaaca aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct
     9061 tgttttgctt cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt
     9121 tggatcattt cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga
     9181 atgcccattg cttttgctgg tggacgcaaa gtactgactt tcttaagcat tgcccgatat
     9241 ttttttgttc cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca
     9301 cttgtttttc cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct
     9361 ccgattgctt ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc
     9421 ggtaacatgt tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt
     9481 atcctcaaat gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta
     9541 ttcgtgctca aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg
     9601 gatcaattga tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata
     9661 ccactgtccg ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga
     9721 taaaagcagc tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc
     9781 aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact
     9841 gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt
     9901 atctgaatag atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag
     9961 attcttcaat tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag
    10021 ataattctct aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt
    10081 atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg
    10141 tgttttttct tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata
    10201 tggattattc tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa
    10261 tcctaatgtc tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc
    10321 tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt
    10381 tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc
    10441 agtcaatgct tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt
    10501 gccactatat gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc
    10561 tacgccgttt aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg
    10621 cgactcttcc tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat
    10681 ttctttgatc ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt
    10741 gtacaaaaac ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat
    10801 ccatagtgcc gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact
    10861 gatataccct agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg
    10921 ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc
    10981 ggcagctaag atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc
    11041 agtgatcatc ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata
    11101 atttgcaaaa cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga
    11161 tcctgctagt gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa
    11221 tttttccgta tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg
    11281 acaccactgc aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc
    11341 tgttcctttt aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg
    11401 cttgctcctt catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa
    11461 ttcgaaagta aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg
    11521 cctttctggt ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg
    11581 tttcacttga ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg
    11641 ctcattctta tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga
    11701 aaatatctct gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc
    11761 ttggaaaaac attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag
    11821 ataatctttt aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga
    11881 tccccctaga tgaggcaaca taagtatacg gtcattttct aagaactctt cttctggaaa
    11941 atcagtcaag tatttagcca gatgttgttt ttccaatgct tgcataacag catatttatc
    12001 aacaatttca cttcttccta cattgataag tacagcatct tttttcattt tttctatatt
    12061 cgcctgatcg atcaatcctt ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc
    12121 cgaggtagaa agaactgttt ctaaatctgc ttgccgaaca taatccaact gggcatcgcg
    12181 gatagaataa cccaagacat ccatccctaa gctatagcaa cttagcgcta cttttgtgcc
    12241 tatcgctcca agaccaagaa gaccgatggt tttgccttgc aattctcgtc ccacataagc
    12301 acttcttttg ttttctgcct gttctaaaat attaggacct gttagtgttt gtaccatacg
    12361 acttgcttcg attatcggac gggaagataa gagcaaacag cacaaaacaa gctcttttac
    12421 tgcattagca tttacacctg gtgtattcat gacgatcgtc ccgttttcag aagctttttc
    12481 aacgttgatc gtgttcaccc caacaccagc tcttgaaatt gctagcaaat ctggtgtaag
    12541 ccattcatct ttcactttcg tactacgaat aactattcct tcggctggca aatcatctat
    12601 tattttgaaa tattcccctt gtaacttttc tatctcttgt tctggaatag catcaatcaa
    12661 atgaacgtga tacataaaac cccctcctta ctaacaagta tctcgaaatt tctgcaatta
    12721 gacaactgtt taccatttga cttcctagaa gcaaaaagag cagttagagc agagtaggct
    12781 gagtgataaa aataaggcta tgacaagtta ctgccataag ccttattttc gagagcaaaa
    12841 tattgagggt gagatatctt gataaatggt gttccgttag ctgacattga taataagact
    12901 aaatttcaaa aaagttgaaa agcaaatttc gtcaatctct tcttatttct taaaactaag
    12961 tggctttttc acaacctctt tttatctaat aaccactgtt tccaactcaa ctaattttgc
    13021 ccacgtttca atttgatctg ttgttagatt tagcgagaca acggtatgat gaccgccacc
    13081 tgcctgaatc catgaatgga caccttcatg gaaattcggt ttagttttcc ataacacacg
    13141 tgcaacaggt aaattaggtg cttcagtcgt tggttcaaag gcttctactt cattgatcag
    13201 cagtttataa tgcgtaccga aatctgccat cgatacgact accccttctc ctgctttccc
    13261 atcaaataca agacgcgccg gatcttctct atctcccatt gataaaggag aaacaacgat
    13321 tcttggtttc gtcgcagcaa gcgtcggatc tacttccatc atatgggatt ccaagattgc
    13381 ttcttggcca ctagctagct cgtaagtata atcttccatg aatcctgtat ctttccctcg
    13441 agccatgatt ttcagcaacc gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa
    13501 accgtatcct tctgccatca aacgttgtac agctagtcca ggtagttgtt tcattccata
    13561 caggtcttca aaattagtcg taaaagcgtt atagcctcct tcttctaaga aacggcgaat
    13621 accgatttcc tgctgagctt gtacttttac atgttcctcc catgtctttt cttcataatc
    13681 accatagtcg aaggtataca attccttgta ctcttcaaat aattgatcga cttcttcgtc
    13741 tgatacgcga tcgattactt gtacaagatc tccgatacca aaataatcaa cagtccaacc
    13801 aaattgaatc tgtgcttcca ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc
    13861 accaaaacga gccactttta ttccaaaact ttcattatag gctactgcaa catccatcca
    13921 atcagcaatc tctttttgga tctcaggtcg tttccagtag ccaacgacga ttttattttg
    13981 tttatttaat cgagcattga tgaacccata ttcgcggtca ccatgagcgg attgatttaa
    14041 attcatgaaa tccatatcga tcgttttcca aggaatgctt tcgttatatt gcgtagctaa
    14101 atgaagcaat ggtttttgta gaagctttgt cccgcggatc cacatttttg ctggtgagaa
    14161 agtatgcatc caagtgatca cacctgctac ttcttctcga taattgacct ctttcatgat
    14221 ttttgtgatc gtatcagcag taactgctaa ttcttgaagg cggattggat aaggcaattg
    14281 gccattttca ttcagttcat cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc
    14341 ttcttcccca tataaatgct gtgatcctac aacaaaccaa aattcttttt ctccaatatt
    14401 caacataatt agccactcct tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt
    14461 tcgtaaataa tgtttatcta aaatccgttg aggcagctct tctgaaaatg aattgagctg
    14521 tctggtaaat aaattcatct tgcatacttc atctagtacg actgcgttca ttaccgcact
    14581 ttgcgcatct ttcccccaag taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc
    14641 taagggatca attttgcgtt ctttgaatgt ttctatgata acttttcctg tttcatattc
    14701 gtacccgctg tcgatttcct gttgtgtcaa aaatcgagca caaggtaccg atccataaaa
    14761 agtatctgca tgcgtcgtcc ccatcgcagg aacatccaat cctgcttgtg cccagatcgt
    14821 cgcccatgtc gagtgagtat ggacgattcc tccgatttga ggaaatgaac gatataaaac
    14881 agcatgagta ggcgtatccg aagagggatt gagtttacct tcaatgatat gattgtccaa
    14941 gtccacaacg accatgtcgt ctgctgtcat ctcttcataa ctgactccgc taggtttgat
    15001 aacgaaatat cgtgtttcag gatcaaaagc gctcacattt ccccatgtat atttgacgag
    15061 tccgtgttta ggcaagtcaa gatttgcttg gtatacttct tctttcagtt gttccagcat
    15121 tttctatcac tcctttttct tgcaattgat gaatcgctgt tttttcaatg tcgatcccct
    15181 cacggtaacg tctcataaat atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg
    15241 tgattccttg gtcttgtcca aacacgtctt ctgccaagaa gttttctaaa gacttcccat
    15301 gtcgatccat catataggct gctaataacg cgattcccca cgctccacct tctccggctg
    15361 tgtccatcac tgtaactggt gcctccatag cagatgctag aatccgctgt ccaacttctg
    15421 gtgttttaaa aatcccccca tgccccacta aacgatcaat ttggacattc tcagatttca
    15481 atatatccat tccgattctc attgcaccaa aagcactggc taaatggatc cgcatgaaat
    15541 tcgctaaatc aaaacggcta cctggtgtac gtacaaataa tggccttcct tcatttactc
    15601 ctgtaatgtt ttcaccggaa aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc
    15661 cttctagcgc tttgttgaat aataccgtga ataatttatc tctgcttatc tccataccca
    15721 aagaattggt aaattcttca aaaatactga cccaagcatt gatatccgaa gagcagttat
    15781 tcgtatgaac catgcctact agttctcctg aaggtgtagt gaccatgtcg atttccggat
    15841 ggacattttc tagttctttt tctaatacga tcatagcgaa ggcggaagta ccagccgaaa
    15901 tgttccctgt ccgtttggct acactgttcg tagcgaccat accggttcct gcatcccctt
    15961 ctggcggaca aaccgggatt cctggtctca aatttcccgt agggtcgatc agataagccc
    16021 ctgattcgct taacttgcca gcttcttctc ctgctactag aactttaggc aaaatgtgat
    16081 ccaagtcaat cgctacgtgc gctttttcga ttaactgacc aaaaatattt ttcatttctt
    16141 gatggtaggt tttcgttgaa ggatcgatag gaaacatgcc tgatgcatct cctacgccta
    16201 gtactttctc accagtaagc tgccaatgga cataacctgc aagcgttgtc aaaaaattaa
    16261 tttctttcac atgttcttct ttattcaaaa tagattgata caaatgagcg atactccatc
    16321 tctgcggaat attgtattga aatttttccg ttaatattcg ttctgcttct tcagtgattg
    16381 tatttcgcca agtacgaaat ggtactagta actgttcctt gctatcaaat gctagataac
    16441 cgtgcatcat tgcactgaac ccaatcgacc cgattgtcgt aagattctcg ccatagattt
    16501 cgaatacttc tgctgccatt tttcggtaac tgacgcgcag tcctttccaa atatcttcta
    16561 atgaataggt ccagatacca ttttctagtt tgttttccca ctcataacta ccagcggcaa
    16621 taggagtaaa agatgaatca atcaatacag ctttgatcct agttgatcca aactcaatcc
    16681 ctaaagatgt acgaccatct atgacatcta acatactttg cgcatttact tccttcataa
    16741 tgatttaact ccctccttct cgtcaaacct gatcaaaccg tctttttagt agcgcttaca
    16801 atgattattg tatacttttg tacgtacatg tcaagcggtt atttacaaga atacaataaa
    16861 aaacacttgt tttgtacgta cataagttat aaaatgaaat tgcttacaaa atctgatcaa
    16921 ccgccttctc gaaattctag aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct
    16981 catcgtttat ttattttttt ggcgcatttg gcggcattct atttggatat gatattggtg
    17041 ttatgaccgg cgccttacct tttttacagc atgattgggg tttggcaggt aaagcttctt
    17101 tgataggatg gatcacgtct tcggtcatgt taggtgcgat ccttggcggc tctttatctg
    17161 gtcttctttc tgataagctt ggacgacgaa aaatgattct tctttctgca cttattttta
    17221 tggctggttc tgtcctttct gcatctgccc ctcacaatgg ttcttatttt ctgattgcag
    17281 caaggattct tctaggttta gctgttggcg ctgcatctgc cctagttcct gcttatatgt
    17341 ctgaaatggc tcctgctaga ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg
    17401 cttctggcat gctcctttcc tatgttgcag attatttgct aaaagtgttg cctgaaacga
    17461 tggcttggcg cgtgatgtta gggctcgctg ctgtccccgc gctgatttta tttttcggtg
    17521 ttttagctct accagagtca ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa
    17581 aaagagtatt gaactatatt cggacaccaa aagaagctga acaggaattc gaacaaatcc
    17641 agctgaatgt caaacaagaa aagacaaccg gaacttcttg gcatactttg tttttagaaa
    17701 aatatcgttc cttagtcatt gccggtattg gtgtagcagt attccagcaa tttcaaggag
    17761 ctaatgctat tttttactat attccgctga tcgtagaaaa agcgactggg catgcagcga
    17821 gcgatgcatt gatgtggccg atcatccaag gaatcatttt agttgctggt tctttgctct
    17881 ttttggttat tgcagacaag ttcaatcggc gaactttatt gaaaataggc ggtagcgtga
    17941 tgggactttc attcatccta ccagctgttt taggaactgt actggatgct catacaaatt
    18001 cgttgttgat tctcttgttt ttatgtattt atgtcgcatt ttattcttgc acatgggctc
    18061 cactgacatg ggtaatcata ggcgaaatat tcccactagc tgtccgcggg cgagcatctg
    18121 gcctagcctc ttcttttaat tggattggtt ctttcctagt tggcttgctt tttcctgtga
    18181 tgactgcttc tatgtctcaa gaaatcgttt tcggcatttt tgggatcatc tgctttttag
    18241 gggttttatt tattcaagag atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg
    18301 aacaaagtgc atctaaaaaa acttatccta aacgtatttc tatccatcat tcttaaagag
    18361 gttgtgacag aagtggtcag cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca
    18421 aggaacaatt tcaaaaaaca gcttctcatg tttgatgaat attgtgactt gtgaccagtt
    18481 ggtaggagaa caccgtttag tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga
    18541 caaaagctct cacaaacatt gcgacgagta accgtaggag caataagagg cggtaaaaac
    18601 ccgtctctta tttttcgttt tctttcactg tttcccgttc aactagtaca ggttcataga
    18661 gaatatttgg ctgtgcctgt cctttttcaa tcgttgtgac gatccaccgt gcggcatctt
    18721 gtcccaactt ttcttttgga tgcgtcaatg ttgtcaattt tttattccct aaatggctta
    18781 aagaagaatc gtctgttcct acgatcgata ggtcatctgg aatatgatag ccattttctg
    18841 tcaacttatc tagcattaaa ctagctattt gatcgttata gcaaatgatg ccatctaaag
    18901 cttgttcttc tttcaaccgt tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg
    18961 tgaaagtaat gatatcttcc gtcaaaaaac gcacattata ctgttcacac gcgctgataa
    19021 aacctttcat cctgtatttt ccttgaagat cgtcgatttt tgtgatgaac aacagacgct
    19081 gatgtccttt ttttatcaaa tattctgctg ccataaaacc gactttcaca tcatctaaac
    19141 aaatataaga ggcatttaac tcttcataag cagcattgat catcaccatc ggaatgcctt
    19201 gttctctcaa agcaacataa agtgctaagt ttgggttata ctgattactt ttcgtaggct
    19261 caacgatcaa accatctacc ccataagcaa tcattttctt caaacactct ttctccaatt
    19321 cgtgatcgtt attcgtactg gctaaaagca atgaataccc agattctcgt aattcatttt
    19381 cgattcctcg gataatcgac ggaaaaatat aatcagagag atatgtggtg atgacaccga
    19441 tcgtttttgt tctgttcaat ggtgtttttt ctttcttata gctgtcatct acatatgtgc
    19501 ccgacccttt ttctttacgc agatagccct catttaccaa cacagcgatt gcttgccgaa
    19561 tcgtatgtct gctgacgtca tatatttctt gcagcttgaa ttctgaggga atggttgtat
    19621 ttacttcgta ttcctcacga aggatctttt gttttatctc ttctgcaata tcttgatatt
    19681 ttgccatttg ccttgcccct ttttatttgt acgtacatat atcataacga gtctgcaagg
    19741 tttattcaag caaaaatcaa ggtttcttgt gctcataagg catacggaac aggttcacca
    19801 aattctggaa atccttgctc attccacgta aagcattgcg cgttcgcgtg ccgattagga
    19861 ttgtctaacg gatccccttt ttggttcttc tctggtcgag catgatagat caaaatatca
    19921 aagcacccgt cttctgaaac cgtaaacgag ttgtgaccag gaccaaattg cttatttttt
    19981 tcagaagaaa cgaatactgg ctccgataat ttatgccatg attcttttaa taaaagattc
    20041 ttgtgctcat cagcccacag caggcccatg gcatagtttt catctgttgc actaccagaa
    20101 tacgtaatga atattttccc attgcgagta aggactgctg gaccttcatt tactgaaaaa
    20161 ccccgttttt cccattcata ctcaggtata gaaaggagga tctgcttccc ctttaacgtc
    20221 caaggattat ccatttcgga tagataaaga ttagaattcc cagtaatttc aggatcttgt
    20281 tgggcccaca cataatatag ctttccttga gaagagaaag ttgttccgtc taaactgaaa
    20341 gtttcccttt tcgtttttat ttgtcccttt tctatccaat ctgtattcat tggatctata
    20401 tgctgatttt caagtacgaa catgcggtga tgatgagatt catcacgtat agttttatga
    20461 ttgcttgcag caaagtaaat ataccatttt ccattgatat aatggacttc aggtgcccag
    20521 atcaattcac tcatcggtcc ttcttcatga gcctgccata caatcaggct tgcagcatgt
    20581 tctaattcat tcagtgactt tgcccttctt aattcaatca cttggtagcc tggaaccgat
    20641 cctgtaaaat aataataacc atctgtatgt ttatataccc aaggatctgc ccgttgccga
    20701 acgataggat tattataaag tgtcttcatc ataacaaatt tcctcacatt ttctttattc
    20761 taattacatt ccaagagtat ttttctatcc ctacatttaa ttcgtcatgt ttttctattg
    20821 aaaaatagtt tatttcaggt tttatttttt ctggttcttc acgtgtattt ttgtccaaca
    20881 gatcgctact ggttaatgtg tagtgctctc cttgatctag caaattgaaa tcatgttcaa
    20941 tcgttatctt ttgttgttca tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt
    21001 tatctacgat cacactgtcc acataaggaa cattttttat cttttcacaa tcataaacag
    21061 ggcaaatgat ttgttgtttc aatacttgac ctttagcata tttcgataag tgttgcatca
    21121 cataataagt tggattcctc catgcttgtc ctttagcatc tgtcagtatt aaaggtattg
    21181 tgtttactaa caatgattga caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca
    21241 gccctaatga accaaataat aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac
    21301 taccgacttc aaaatcccta tgtctttttt ccggttgggc atgtacattc cattcatcaa
    21361 acgctagata cattgttttc tcacttctca aatatccttt tacataatca caagtagcaa
    21421 cgatttcttc aatttgacga tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat
    21481 cacgctcatt tttaggaggt ttagtcaagt cttcttcatc atacttatca atatagttat
    21541 gcaaagctaa ataatctaca ttctcataag cttcttctaa tgcgatacga tcccattcag
    21601 ggtacgatgg taaacgtggc gtagagcttc caactaagat tgtttcaatt gaatcatcca
    21661 ctaacttcat gacttttgag gcttcatttg ctaacctacc atattcataa gcactcttag
    21721 cccccacttg ccatggaccg tctagctcat tccctaaaca ccataattta acgccaaaag
    21781 gctttttttg cccatttttt attcttaaat cactccagaa ggttcctttt ggaaaattac
    21841 aatattctac taaatttttt gcttcatcaa ttccttttgt tcctaaattc accgtgaata
    21901 tcggtttcgc ttgtacatga ctgatccatt taaaaaattc gtggagtcca aaagaatttg
    21961 gttctatacc tcgccatgct aaatcaattt tttttggtcg ttgttctttc ctacctacag
    22021 tatccaccca atcataacca gatgtaaagt ttcctccagg atagcgaatg acacctaatt
    22081 ctaattcctt tacataagag aggacatctt gtctaaaccc gtcaccatca gctgtttcat
    22141 ggcccggttc ataaatccct gtatagacga cacttcccat atgctcaaca aatgagccaa
    22201 acagccgaga actgatctct cctaccgtaa aatcctctct gatttttact ttcacttctt
    22261 cgtccaacag ctttccctac ctttcatttc ctatagtcaa gataatagat acaagtttcc
    22321 atacgccacc agcaaagata aaaataaaag gagggaaaag aaatatgagc agtacaaatc
    22381 ctaataacaa tacgcaacaa agaatatttc tcaaaaaatc aaaaaatact ttccatgcac
    22441 tttgttcaaa aatttgtaaa aatgtgatat ctttgttttc agaaatcaca tatatccagg
    22501 ataaaagaaa catggaataa ataagtaaaa ctacatatcc actaattgaa agtacgattg
    22561 tctgttcttc taataattga ttaacaagaa aataaataat gatcgttaac agactaagga
    22621 acaagttctt accaaaatgt tgctttattc cttgaaagaa aaaataaatg atatttcctg
    22681 atccctcacc tttccattgc gccattacat aaaaaacagc acatgtagca ggcacgatag
    22741 taataacagg caaacacacg attacccaga gaatacctaa taagatgtaa tcagaaacca
    22801 caatcaactg tccataaaat ttagtctcaa ctattttcat cttcctcact ctttcattcc
    22861 agacatagct gttccttgaa caaaatattt ctgtgcaaaa atataaacca ctagtaatgg
    22921 cagcattgaa ataaccgttc cagccattac aggtccataa aaagaaatgt tctgccctgt
    22981 aaataaagct aaccctgctg gtaaagttct catttttgaa ctacttgtca atatcaatgg
    23041 ataaagcaag tcattccaac tattcatcag cgtgaaaatc cctaatgata aaagagtagg
    23101 tttgctcaat ggcaacatga ttttcaaaaa aatattaaac tcacttaatc catcaatgcg
    23161 tgctgcctct tccaaagaat ctggtaaagt aataaaaaag gaacgcatca tgaagatacc
    23221 aaatgcagtt gtcatacgag gaatgatcaa acctgcataa gtatcaagca tgccaagcaa
    23281 attgacttca ataaatagag gaatcatgaa gacttggaat gggatcatca ttgtcagcaa
    23341 aacgaaatag aacaaaactg attttccttt gaaacgcata cgagcaaatg cataccccgc
    23401 aagagaatca aagaatacag aagtgatcac tgtccctcca gcaaaaatca ctgtattttt
    23461 gatgtaatct aataaaggga tcgttgtcca aaccttaata tagttatcta gggtatagct
    23521 atctgcaaat attctaggcg gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat
    23581 aatcagccac aaaaaaggaa acgcaataac caacaatata actagcatac taatcgttcg
    23641 aatgattttt ttaccactaa ctgcttttcg actattcaca gtaactgtaa tcgagctttc
    23701 tggtgccata caggtttccc tcctatttca tttgtttttc tttccgtgac atgtacacat
    23761 tagtggagat agctatagtt gcaacaatga taaacaaaac cgaagacagt gctgatgcat
    23821 agcccaattc ataaggtgct gtaaatcctc gactgtaaat atattgaaca gctgtttctg
    23881 tcttaaattg tgggccacca gcggttgcaa catatacttg gtcaaacact tgcatcgaac
    23941 caatcagatt cgttaaaata caaaaaccta aggagggaac gatctgaggt agcgtaatgt
    24001 taaaaaactg ctgtcgattt gttgcaccat ccatttgagc agattcatac aaagagggag
    24061 agattccctg aatagcagtc aagagaatcg tcatagtaac accaaaattc ttccaaacag
    24121 tcatgatggc taccgtcggc atagcaaggg ttggatcgcg aaaaaattga gggatcggca
    24181 agccgacttg tgaaaacaag taaggaatcg cccctacatt cggatcaagc aacatagaaa
    24241 aaataatcgc aattgacgtc aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa
    24301 aacgattgaa tctggtattt ttagataatg ctactgctgc taataaacca acgataattt
    24361 gtgttggcgt ttcgagtacg gtgaaatata aagaatgaat caacgaatta actgctcgtt
    24421 catcactaaa tatacgtgtg aaattttcaa aacttacaaa atttcttgat gtaaagaaaa
    24481 tattcaaatc tgtaaaacta atagcaaatg ttcctaaaag cggtatgaca gtaaataata
    24541 ataaaactaa aaaagatgga gttaaaaaca aatacgcagc acgatcagga cgattccaat
    24601 atttggtgaa tccgttcata tagtccacct catctttcat tttgataaaa tatacaatct
    24661 tggtttaaac gaatctgaat aagatatccg aacaatcgct gtgatttttg caataacacc
    24721 tatctgcaaa tactaggtcg atcattcaga tatctgtatc ttgccaacag tctatcctgt
    24781 ataaagttct gtactatttt tgcagtaatt gattgatggc atcatctgca tcgttcatga
    24841 gtgtctcagg atcttctcct gctaataatt tttcaatcag tggattgatg atatcgttat
    24901 tgatcgcaga gatttgggta aaacctggta aatatggctc tgcatagtcc atttgtttcg
    24961 ataattcaga tacaatagga tcatttttta cttcttcatc ttttgctact gaatccaagt
    25021 aggcaggaaa accattttcg atactccatt ttttaccaat ttcagttgta ttccaatatt
    25081 tcaaaaactc atagacagct tcttttttat tattatctgt acttgccgga atggcatatc
    25141 ctacgccatc taaaatagct gtttttttgt ctgaatcact cccttgtggt aaggtagtca
    25201 cattataatc aatctcgttt gctttcaatc cgttatttaa ccatggccca ttgatttcaa
    25261 tggctagttg accagcattc attaaattgt ccatttcggc gccagaaatc gattctggac
    25321 cagtcttgtc tacatggatc attttttgga tcgcttttaa cgttttaagg ttctcaggag
    25381 acgcaaattc agacttagta taatcatcgt tcactaattt accgccattt gctaacatcc
    25441 agttatataa cggtgcagtt ccatccttat tgaagacaaa acctgataca tttttactac
    25501 tatcagttaa ttttggtgcc atttctgtca attcttccca agtagtaggt ggttgttcag
    25561 tatctaatcc agcttgttta aacaaatcat tattccaata catatacatg ccttgtacct
    25621 gcatggggac aaaatactgt ttaccttcta cttcacctaa ttcaatagcc gtttctttaa
    25681 aatcagattt atctactcca tcatattccc agaaatcgtc taatggttgt accgctccgt
    25741 tttctacata ctgtgcaaaa tctggataat ttaatgcaat aaagtcaggt gcttttttag
    25801 aagaaatcgc tgtaggaagt ttttcattca aattggccca agtcatgata tccatttcaa
    25861 ttttgatgtt tttatcattt gttttattaa attcattaac gatttcttgc aatatttcac
    25921 catcagatgc agtaaaccca ttccaaaaag tcaaagtcac ttctttatca gaatcagatt
    25981 ctgctgaact tccgccaccg caagctccta aaatacctgt tgcagctacc aacaaagagc
    26041 caattaacca ttttttacct ttcatttttt gttcctcctt catctgataa acgacatctg
    26101 accacattcc aagatagcgg tttcaataaa caattgattt tcccatcttc agcttttaca
    26161 tttgttgaat gacttggttt tacttgttca cttttaggaa gattagttgc ttttgtatca
    26221 aatccactca tttcagaaaa atcaatcact ccctctaatg ataattcaga caagtgaaca
    26281 gttagattca tttcttcttc tccacggtta actgcaaata tgacaagttc tttgtcattt
    26341 attacggcta tactttctaa atacggcact tcttcaaaat cttccgtcgc atagtttgct
    26401 gaacgaattt gtgggcttaa cacctctcct tgtccataca cagcagcttg cataaatgga
    26461 taatagatcg tctgtctcca agcttctccg tttgtttctg tcctgattgg tgcaataacg
    26521 tttaccaatt gagcaagaca agcaatcttt actctatctg catgtttcaa taaagtgatt
    26581 aatagacagc ctaatagtaa tgcatcttca aaattatagt tatcttctag taaaggagga
    26641 gcaatctgcc aaggcgctgc ttgctcatct tgttccgctg aatgatacca aatattccat
    26701 tcatcaaagg acaggttgat ctgcttctta ctatgtttct tcgccttgac tgtatcgcac
    26761 atcgctgtca cttcagaaat gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa
    26821 agatcatttt tttggttgcc ataatattga tgcagagaca aataatctat ttgatcgtac
    26881 gcttgatcaa gaactgttaa ctcccaatca ccaaatgtag gcatttgccg attcgaactt
    26941 ccgcataaaa ccacctcaat tgaatcatct acgagtttca tcgccttagc cgtttcctct
    27001 gctaaccgtc catattcttc cgctgtttta tgtccaatct gccaaggacc gtccatttca
    27061 ttccctaaac accatgtctt aattccaaaa gggctctctt gaccattcga acgccttaaa
    27121 tcactccaat aagttccttt aggaaaatta caatattcga ctaaattacg agctgcatct
    27181 actccacgag tacccagatt gacagccata ttgatttcgg cccccacatg ctgacaccag
    27241 ttataaaatt catgaatccc aacttcattc gtttcaattg tccgccaagc taaatccaac
    27301 cttctaggtc tatttgctat gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa
    27361 tttccaccag gatatctaat catgggaatg ttcaattctt ttaccaagtg aatgacgtct
    27421 tggcgaaatc cacttttatt agctgatgga tgatctggct gataaatccc ttcatatact
    27481 gctcgtccca tatgttcaat aaatgaactg tacaaccgcg gatcaacttt gcctacacgc
    27541 ttgacactac taatcaacat tgtggcttcc ataactgacc tcctttaatt tatacatgaa
    27601 taatacacct cgttgaaagc gttgtcaata atataaatgt taaacttact tattttttat
    27661 cgattttaat taaccaaaat attaataatg attaaaacaa ataatttaat ataactttta
    27721 ttaacaaata tgttaatata taagaaaaac cttttaagag gtgatatata tgcaactaca
    27781 aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa ggatcaagat
    27841 catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag aacttggagt
    27901 gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca tcatcaaaac
    27961 agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg atattagttt
    28021 tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta ttggtcacta
    28081 tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt ttatcgggaa
    28141 agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca ttctctggtt
    28201 taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg atacattgga
    28261 gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg acaattggcc
    28321 ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga gccctggaga
    28381 ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc ttaatcaata
    28441 cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg gagaaccctt
    28501 gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt tgcgaatcga
    28561 agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag gattcggtaa
    28621 ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt ttctatttta
    28681 taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat tgttatttcc
    28741 agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg ttccaccatg
    28801 cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa tcatcaattt
    28861 cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca tcttcaagat
    28921 ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga agcaattgtt
    28981 tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca tcaccaaatt
    29041 tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg gctcctgctc
    29101 tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata tttcctttgt
    29161 taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca tcaacagctg
    29221 gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca gcttttgcat
    29281 cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga tagttttcat
    29341 cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct gctaaagtag
    29401 aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat tttttcaaat
    29461 atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt gttggtttga
    29521 cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct atgcttctgt
    29581 atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat ggttccagca
    29641 ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat actcttccca
    29701 tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag gtaattcgta
    29761 aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa agtctcgaac
    29821 agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt cttctcgagg
    29881 gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat ttcgagtgat
    29941 gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg ataacttgat
    30001 cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata ctgcaccaag
    30061 ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat caattttagc
    30121 tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag catagatttt
    30181 tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata tataggccat
    30241 atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt ctttcgccac
    30301 gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta agaacacgca
    30361 gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat aagattggac
    30421 taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag cacgtacatt
    30481 gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg ctgcatccag
    30541 ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga cagatttgat
    30601 acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca gtgctgtttc
    30661 tactaattgg cagtgcgcta aaggcccttc cacttggatc aatggttcat ttgcaaaaac
    30721 tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt gtaaatactc
    30781 caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg aatcattgaa
    30841 cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg cgtatccgtg
    30901 gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag ctcgacctaa
    30961 ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg tcaaactgtc
    31021 gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca tttcctaccc
    31081 attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa aaagcacatg
    31141 ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc tttcttattg
    31201 gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag acacgaagaa
    31261 attagcgttt tctatttttc ttacttgata aaataagtag attttcctac tacaatcgta
    31321 gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac ataatcaatg
    31381 gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc ccaaagaaac
    31441 ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca agatatacct
    31501 tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac aaagtaagaa
    31561 agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct cgccttgttt
    31621 tttattgtat acattagata tggagcaggt taccataaag atgattatta gtcgttatta
    31681 aatagaatcc ttcgtatttt tttgttctat cactacaata tctttatttg gacgtttgaa
    31741 ttgtattccg gcacgttcta gctcctgttt cactcctaca tggactttga catcttctcg
    31801 tgtcattttt aaaacgtgat gaatcgcata gtcgatttct tgccttgtga tttttttctt
    31861 cgtattgatc atcacgactt gctttttatc atatgtgtct accaatagca gttgtgttcg
    31921 atctaacgaa aataatttgt aataattaat agccgtattc ccaaaaaaga attccactac
    31981 tttaggataa aatgtcttaa tatttaaatt actattcaca ggtgtttcga ttagcttcat
    32041 ctcattcctc ttttctatat agtcgattta gatccaatct taagttatgt taccatcttt
    32101 cctagcatta aggaatcggt tacaattgaa cctttaaaga caaaaggatt taccttttat
    32161 cacttaatag tataacataa tttcatgaga aaaaaataca attttttctt gaccatctcg
    32221 ttacgtaaag gattatgctt acatttgtca ggaggaaaaa taatggagta cacaataaag
    32281 aaaatgtcag agatatcagg agtcagtccg agaaccttgc gcttttatga tgagattgga
    32341 ctgctaaaac cagctaggat caattcttca ggttatcgga tttatggcaa aaaagaagtc
    32401 gatcgtctgc aacacatctt attttatcga acaatggcat tcaagttgga tgatatccaa
    32461 gaggtcttag acaatccatc ttttgaccac cagaaagctt taatcaagca tcagcaaatg
    32521 ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa ctgtccaaca gacacttgat
    32581 atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat ttgaagggtt taaacaacaa
    32641 aagttgaaag aaaatgaaga atcctatgga caagagattc gacaaaaata cggaaaaaaa
    32701 aaggttacag catccaatga aaaatggatg aatatggata aagaaacgta tgaagaaatg
    32761 caaactgttg aaaagcagat gttatttgat ctcactcttt acttaaaaga accagaagat
    32821 gaaagcttag cagaccgtat cttccaagct cataagaagt ggttatctta cagctggcct
    32881 gaatatcagc caaaagcgca taaaggacta ggaatgatgt acgaagccga tgaacggttc
    32941 actgcatatt atgacgagcg aagtggtcat ggtgcagcaa aagctttaaa tgaaattatc
    33001 caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag tacacgtaaa atgacctaat
    33061 aacaaaacag cagacaccca agttagattt ttatgctaac ttttggtgtc tgctccattc
    33121 tttcacaagt tttttcatcc tactttaaat atattcatcg ttgaataaac gtataaggta
    33181 ttttcacttt atcctttttt tctgtaagaa acaattgaaa tgccatttct ccgcaagctt
    33241 ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct gatcagcaaa ttatcgcgtc
    33301 caatcaccac tggtgtctta tcttgcagat agctttgcaa gatgcctgct gcaaccatat
    33361 ccgaatttgt aaagacacca tcaactccaa gctctttaaa aaagaagcct gcttgttttc
    33421 ctttctctgc atctatacaa tcccaaaaaa caagtgattc atcaaaagat ggaaaaagtt
    33481 gatgacatag ctgcaaagtg atttttgagt tataacttag cctgccactt cgccccaacg
    33541 tgatgcccaa ttgctttata ccttgctgct tcaaataagt aagaccttct gtaattgatt
    33601 gttctcgatc aataaagaca cagcttgcct gttttccctt gatttcttca caaaaaacta
    33661 gaggtccata tttttgatat gccaataaat catctagtgt attggcacga gacgtaatga
    33721 tcaatccatc gaaagcttta gctgcaaatt cttcaagata ccttcgctct ttttctttct
    33781 gatagcccgt cggtaaaagt gtcactttgt aatcataatc aaacgctgct tcaatgatcc
    33841 cactgatcaa ctgatcaaaa tacggatgat tggtataagg cagaatcact ccaatgcttt
    33901 tcgtttttcc ataacttaac tgtctggcat ttgcattagg gatataatcg acttctttca
    33961 tgaccgcaag tatttctttc cttttttcct catctacata tggatgtcca ttgattacac
    34021 gtgaaatagt agaaactgaa taacctgtga gctttgcgat atctcgaatg tttgccactg
    34081 ctgcttcgcc acctatacaa aaagattttc ttgtaaatag tctaccacag ctggaccgca
    34141 ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg atccgctgcc cgccccaata
    34201 tatatctttt gtcaaatttt cgatttcatt cattggtatt tcttttacta tattcctatt
    34261 caacccttga aaactgtaca acgttaatac ggatcccttg aactcgacaa acgcataaaa
    34321 ataattgttc gttgtgccat ctgttatctc tacataagta ttcattgcaa gccctccttt
    34381 tgatactttg agtatagtgt atgaaacgcg tctcatagca aggagtttgt tttgccaaag
    34441 aaaagtttta ttcctctttc attattacca aagtctctta ttttaaattg aatacattat
    34501 aatgatactg taaatatatc tttattatta gtgaatggat atttactttt aatagataaa
    34561 atattataac tattaaaaat aactattttc attataatta cacacaaaaa agagcgtgag
    34621 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacagc ggaagcagaa
    34681 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt tccgtgaatt
    34741 tcttcttatt tcttggagtt aaacgcttct gttccagcct ctttttatct gtctttcact
    34801 aatgatcagt tgtagataag caagttgctt tttcttacat cataccgccc atcattgatg
    34861 gatccattgc aggagctgct ggcgctgctg gttctggttt atctgcaacg actgcttctg
    34921 tcgttaataa caaggcagaa acggaagcgg cattttgcaa ggcagaacgt gttactttag
    34981 ttgggtctac gatacctgct tcaaccatgt ttacccattc tccagtagca gcattgaaac
    35041 cagtgcctag ttccacgttc ttcaatttat caacaatcac tgatccttca taaccagcgt
    35101 tttcagcgat ttgacgaatt ggttcttcta aagcacgaac aacgatcttg atacctgttg
    35161 ccacgtctcc ttctgcttct acagcagaaa ctttgctgat aacgttcact agagctgtac
    35221 caccacctga gaccattcct tcttctactg cggcacgtgt agcattcaat gcatcttcaa
    35281 tgcgtaattt taattctttc aattctgttt cagtagctgc accaacttta acaacagcta
    35341 caccacctgc taatttagct aagcgttctt gtaatttttc gcggtcaaaa tcagaagttg
    35401 tttcagcaat ttggtttttg atcaattgca cacgagcttc gattgcttct ttttctccag
    35461 aaccttctac gatcgttgta ttgtctttat ctacaacaac tttagaagca tttcctaagt
    35521 tttcgattgt tgcatctttc aattctaaac ctagatcgtc agtaattact gtaccacctg
    35581 tcaaaatagc gatatcttca agcattgctt tacgacgatc gccgaaacca ggagctttta
    35641 cagctactac gttgaatgtt ccacggattt tgttcaatac aagagttggc aatgcttcac
    35701 catctacatc gtcagcaata atcaacaatg gacgtgattg ttgcaagatt tgttctaaca
    35761 atggcagaat atcttggatg ttagagattt ttttgtctgt gatcaagata tatgggtttt
    35821 ctagaacagc ttccattttg tcgttatctg ttaccatata ttgtgataag taaccacgat
    35881 caaactgcat accttctaca acatctagtt ctgtttcgat cccttttgat tcttcgattg
    35941 tgatgacacc gtcgtttcca actttttcca ttgcatcagc aatcaaatgt ccaactttat
    36001 cagaacccga agaaacagct gctacttgtg caatggcttc ttttgaatca acaacagttg
    36061 agatattatg caattcttcg actgctgctt ttgttgccaa ttcgatcccg cgacgaatgc
    36121 ctagaggatt agcacctgca gttacgtttt ttaaaccttc gcgaacgatt gcttgtgtca
    36181 gaacagttgc tgtcgttgta ccgtctcctg caatatcatt tgtttttgat gctacttctg
    36241 atacaagttt tgcacccatg ttttcaaaat gatcttctaa ttcgatttct tttgcgatcg
    36301 tcacaccatc atttgtgatc aatggagagc cgtaagattt ttctagtacg acatttcggc
    36361 ctttaggtcc taatgttact ttcactgtat ctgctaattt atctaccccg cgtaacatag
    36421 ctgcgcgtgc gtcttctgca aatttcaatt cttttgccat gattgattca cctcattgat
    36481 tatttttgat tattttttta tcttccaaat ttactcaaca atcgccatga tatcttttcc
    36541 tgcgacgatc agatattctt ttccttcgta ttttacttct gtaccggcgt atttttcgaa
    36601 cataacttgg tcgcctgctt ttacagcggc aggaactttt tcaccgtttt ctaacaaacg
    36661 gccttcaccg actgcaacaa ctgtcccagt ttggggtttc tctttagcag cagaagctaa
    36721 aacgattcct ccgacagttt tttcttcttc ttttgcgact tcgataatga cgcgatcacc
    36781 taatggtttt aacacgataa atccctccga taactctttt tttaatctgt tagcactctt
    36841 aaccttcgag tgctaaccta catttttaat aatactcatt ctccctcttt tttgcaagct
    36901 tttaaatcaa agaaaagaaa ttttattctg acagctgctt attgaaatta tgatataatt
    36961 gaaatcaatc gatataagct tgccttgcat gcttgttatc ccttaaaagg aggaattaca
    37021 tgtctttaaa aaaatatagc ttcctcagca ttttttgcta tggtctaatt tttatctcac
    37081 ccttgatcct agcttcgata ggacttgtaa aatctacgtc tgaattgatc acagcaactg
    37141 cagtcatgta tatactaggc gccgttgtcc tagctatttt ttattttaag caacgtgaac
    37201 ccttagcgat cgaatcagcg gtaaaaaaag cttctgctcc aaagattgtt atttatggat
    37261 tggttggaat ctttgtcgca ttgattctgc aaagcatcgc tgtcatgtta gaatctgtat
    37321 tgtttggcaa agcaacccct tccgagaata cacaaaatat catccagatg atcatggaag
    37381 cacccgcttt tattttggcg acaactgttg ctggacccat catggaagag ttcgtattcc
    37441 gccgcagtat actggggatc atcagtcgat attcaaattt ttgggtcgga gcagtgatca
    37501 gctctttgct gtttgcgttt gctcataatg atggtcatct gctaatctac tttttcctag
    37561 gtttcttctt cagcctagag tacaaagcaa ctggtcggat ctggacatcg atgatcacac
    37621 acgtaggaat gaacacatta gttgttcttg ttcaattagc tgtccaaaaa gggctagtat
    37681 aaacatgact ataaatactc ctgtcatcct agattggtga ttttcccaat ctaaagcgac
    37741 aggagtattt ttgtttttta gtcgttcatc ttttctgctg ctccatcatt ttttgtctgt
    37801 ctttcctttt tgcagtttgc agaatctatt tttttcaaaa atgggaaata aatgaaaaaa
    37861 ctaattgaca acacgatacc ttgtaaaatc gctgcaggca ctccgcctac taagaaaccc
    37921 gagaggatcg gcggtgttgt ccaagggact tggactgctg taaataaagg aacgaatcca
    37981 aaatataaag cggaataggt aatcaatcct gaaacgaccg gtgcgaaaat aaaaggaaca
    38041 gccatcatcg ggttcatcac gatcggtgtg gaaaaaataa ttgattcatt gatgttgaaa
    38101 aaggctggta aaagagcaag atttcctaat tctctgtatt tcttcgattt cgcaaaaacc
    38161 gtcataaaaa atacagcccc taacgtcaat ccagcacccg taaccgtcat gaactgatcg
    38221 agaaattgct gagtaacgat atgtccgcca tttgccaccg tcaactcttg tcctgaggct
    38281 aagatttctg catttgctaa tgaattcgct tgcaagacgg gactaaccaa cccattgacc
    38341 atcgcagatc catgaagacc aaataaccat aaaaacggaa caaaaaaacc taataaaagc
    38401 acaccaaaca acgtgtctat gaacgattga atgggaagct ggacatatgt ccatactcct
    38461 tgaacaacag tcattctgaa caaatgttga aaaaccgctg aaccgataag cgtaaaagtg
    38521 actataacaa tgattggaat caatgaagta aaagactgcc aaacattttc aggtatcttc
    38581 tctctttgct tccacccttt cgcaagtctt actaaacgtg catagcccca tccgatgatg
    38641 agcccagttg taactgctcc tagtatccct tcagcacctg tccagacagt atctatctga
    38701 gtatttagtg gctgaataag cagtagataa gcagacaagg ctgttatgcc gactggtaag
    38761 cgatcgatgt cttcttcttt tgcataagcg tatgcaatgg caaaaacgat ccaaaaggct
    38821 atcacatcaa atgtagcctg gtatacttgt gtaaaataca tagaaaatcc tgttttgtct
    38881 atccactgag cgatgtaatc cacaggaaaa tgagcaaata agaaaaacag cgagccaaca
    38941 ataattattg ggatcgtgta agtcattcca ttacgcaaag cagttaaggg tctaagattc
    39001 acaacagatg ttatcattgg taaaaaggca cgatctaacc actctttcat ttcttatctc
    39061 cttcaacttc tatttttatc aatcagtttt cttaatttaa ctatacccaa tgatcacgaa
    39121 ccatgaaagg aatgcgcact gagcttttca aaaatcggat tctttgacga atattcagca
    39181 actgcttaca atggacagga ataaaaaagg aggtttttta tgaaaattgc actcattgga
    39241 gctactggcc atgcaggttc atttatttta gacgaagcat tgtccagaga tttagacgtg
    39301 acggcaatcg tgcgtcatcc agaaaaattg ccatcagatg ttccgtttat acaaaaggat
    39361 ttatttgagt tgacaactac tgatcttgca tcttttgatg ttgtcgttga tgctttcaac
    39421 gctcccaaag gaaaagaaga aatgcatcaa acttctttgt cccatttgat tgagatgctt
    39481 gaaggaaccg atacacgctt aattgtcgtt gggggtgcct cttcgttatt cttagataca
    39541 gaaaagacga ctcgtatgat tgaccaagta cctaaaaatg cacccttcta tccaacagct
    39601 ttcaatatgt acgaaggatt gttaaaattg aaacaatcta aaaatctaca ttggacttat
    39661 atcagtccag cttctttatt tgatccacat ggcaaacgta caggtactta tttgttaagt
    39721 gatgattatt tacaaagaaa cgctgctagc gacagtacga tcagcatggc agattacgcg
    39781 attgcattag tagatgagat actggatgag aaacatgaaa agcagcacat cagtgtagtc
    39841 agtcgataat acggttattt ttttaatatg ctaaaaaaat aaaaatagaa gataaaacca
    39901 accctgattg attttatctt ctgcttttta tatttttatt gattgaactg tttaaatcga
    39961 aaatagctca gttgcttcat ctggatctgt atcatagatt tcaaaatcat gtccaacgcc
    40021 aaaatctttt tctttcaaaa cattttccat tgttaaatga gcaagttctt tcatattgtt
    40081 ttccttgcta agatggccaa gatagattcg tttggtccga ttaccgatca catcagtcat
    40141 gacaagtgca ccgtcttcgt tggaaagatg tcctctatct cctaaaatac gctgttttaa
    40201 attccacgga taaggtccca tgcgtaacat ctcaagatca tgattacttt ccatcaaata
    40261 accatcggca ttttcaatga cacctcgcat ataatcactg caatatccgg tgtccgtcaa
    40321 catgacgaat gaccgatttt ctttatggaa acgataaaat tgcggagccg ctgcgtcatg
    40381 ggagacgccg aaactctcaa tatcgatatc gccgaatgtt ctgactttcc ccatctcaaa
    40441 tagatgtttt tgttccactg gtacatttcc aatcagtggt tccatcgctt cccaagtctg
    40501 ctgattggca aaaacatcca aatgatactt acgagccaag acacccacgc catgtatatg
    40561 atctcgatgt tcatgcgtca caagtattcc atctaattgg tccggtgtcc gatcaatttg
    40621 agccatcaag gaagtgattt ttttaccgct aagtcctgca tcgaccaaaa gccgtttttt
    40681 ttcactttcg atataaagtg aattacccga acttccactt gctaggatac tgattttaaa
    40741 agctggtttt tcttcagcca tttgactcct acttcccttc ttactttgta aacttttttc
    40801 attatactcg cttttttatt gatcttccac ttttggtaca gtattgtttg tcacgatcgt
    40861 attgctgaaa gcgttgatgc tctcaatttg gaccacttta tcgcttgttt cgatcgctac
    40921 atgccaaaca ggaacataca catttttttc ccggactttc agaatacagg aataagctag
    40981 ctgacgccat aaaatttttg atcctcttgg tattttgtta ttgatgtaca atgtattgat
    41041 tgcttcctct tccgtataca gttccgtctt ttcgcgcagc tgctcgatat cagataaatg
    41101 cgtctgcatg tatttcgtga tgctataatc attatcgttt ttttctaaag aaagaacgat
    41161 ttttgctgta tcatcattga atgggatgcc ttcatactct tggcttgctt ggatttctag
    41221 tggttcttct tctaaatgag aaaagttttt cgtgtacaca taatcattac cgaataaaat
    41281 attgtctttg ttagataaaa aatcttccaa ggaactacta attttatctg gatcgatcac
    41341 gttcttttca gcatctgaac tggataaaga tttcgtcaaa acatcaccgt ctaaaaacac
    41401 accacttgcc agcaagccgc ttttaccagc cttgcgataa ttcgctaatg aagtgctaag
    41461 attggtctgc tccgcgctaa gataatagcc ttgcttgttc tcagacgaaa tcttccccgt
    41521 gtaagtgata tcgtcgctag ccagacgttg ttggattgat tcagtctgat tagagcgaat
    41581 gacattgttt tcttggtata ccgcgtcatg ataactgctg aataaaaaca cattcaaccc
    41641 taaaaaagcg acgaaaaata tccattcgat ttttttgaaa tccaattaat tcgcctccaa
    41701 tccaggtaat ttttccagaa ggtcgttagc tgaataccag ttatctttat atttcacata
    41761 ccattcaggt aagagatcaa cgacacgatt cgtttctttg acattttgcc atgtatagcc
    41821 aacgatcact gaaccaatct tgttgctatc tgctccctta gcttttaact gctggatgat
    41881 ttcattcgta cttgcaagtt ctacttcttc gtctgaaggt atcggcactt gaatcgtatt
    41941 caaactggtt tggatattca cttgcacaga accattcgtt tcaccgccaa cttccacacc
    42001 aatctgtcct ctgctgtctg tcccaaaaac cgggaagcct tccacgaata tccgataatc
    42061 gatcgtattg tcactgcggt caaaatatct gaggttccct aaagaactgc ctaatttgct
    42121 aatatagggg aaactttcag aaaatacact agcattttct tttttctctg gcagctctcc
    42181 acgaaaatca actacttgcg tttccgaatg gatagacatt gtttcattcc caccgtaaaa
    42241 aatcaattca ttgctttcat cattattttt cacgctatct ggtttctgga aaaaagcatt
    42301 tctaaaaagt gtatagggtt gctgtgacag gatataacta tacttcttca gtttgatcga
    42361 atgtttcgta gcatattgcg tgtcgatcgt cgatggttct gttgtcattt ctgtccattt
    42421 tgtgtctttt gctgttaatt ctttattgat atctgtccaa ttcaacgaaa tatttgcttc
    42481 atatacgaga tggttcgaat aattcaaaaa tcggattttt ttatttcctt gatcgaattg
    42541 tattctagta aaatacatat tttcgctttc ttttgccaag ttcgacacat ctaaatctaa
    42601 atcaaatgct tccttgtatt ctcttagcaa aaaaggcgca ttataggcca actcaaaacc
    42661 agagttcaga tttttcacct gttcaaactg tgatttgtct cctgaaacta cttcagaaag
    42721 ctttccaaat cttgctttat cgatgatcaa ttgcaatcgg tacacgagat tttcactgtt
    42781 ggtttcttgg atcattcccg aatggtgcca tgtaacatga agcggaagaa agacttcgga
    42841 agtttttcga ttgctttgag actcgatcat ttgagtgttt gtctcatcta cgttgattga
    42901 actttttcct gctgggctca gccagattgc ataagagaag tatatgctta acgcaaccat
    42961 caaaatgagt cctatacgaa cgactttttc agagattctc attcccacca ttcctcctca
    43021 taaggttcat atggcagcga gatataaaaa gtcgaccctt ttccttcctt gctctctgcc
    43081 caaatagcgc ctcgatgcgc cttaatcact tctctagaaa tagctaatcc aagtcctgtc
    43141 ccaccttgtg ctcttgcacg tgctttatct actcgataga accgctcgaa cactcggttc
    43201 agatcttttt taggaatccc caatccttga tcggtgatac ttaaaatcac gttattatgc
    43261 gtttctaata agcgacaagt aatcgttccc ccatctggtg aatacttgat cgcgttattc
    43321 atgatattat cgactacttg gatgatttta tctgtatcta cttcgaccca aagatctctt
    43381 tgcgtaaatt cacgacggat cttataattt ttttcctgat ttcccaccat catatcaaac
    43441 cggtcaagaa caaaattcac taactcgttg aaattgacat attctaattg aagctgggta
    43501 ttacctgtat ccatgcgaga tagatttaag aggtcattga tcattcgaat cattcggtct
    43561 gtttcatcta gcgttacctt caaaaaattt ggtgctactt cctcgtcttt ccacgcacct
    43621 tcgctcaatg cttcgatata actacgcata cttgtcaacg gtgtacgaag ttcatgggaa
    43681 acattagaga cgaattcacg acgttcccgt tctgtttttt cttgttctgt cacatcatgc
    43741 aagacagcta ctaatccgct gataaaacct gattcgcgac ggatcataga aaagtctact
    43801 cgcaagatca tctgatcact ttccaatgta ctggttgaac ggtcaagtac catttcgttt
    43861 ggatcttcta ataatttccg caacgtatat tcttcttcga tatctaaaag tgtcaaaatc
    43921 gattgtccga ttgcttcctc gtttttgata tctagcaagg aaacagccat atcattgatc
    43981 gtaatgactt tacccctgcg atctgtcgca atgacgccgt ctgtcatatg ggatagcacg
    44041 ctatccaaac gattgcgttc agcttccatt gtatcttgag cttcttcaat acgttcagct
    44101 aactgattga aggtatcagc cagctgtcca agttcatctt gtccatgcac tttgactttc
    44161 cgactgtagt ctcctctagc gattcgaatg gcttgttctc tcatttcacc gatcggctgt
    44221 gtaatcgaac gagcaactaa aacagatacg atcattgaaa taaatgccgc aattagtgag
    44281 gcagtaaaga aaatgactgc tgtgttattg atttccgaat atttttgttc gatattactt
    44341 ttgacgtaaa gagccccgat aacggcatcg ccggtcgggg acaggatcgg ttgaacattg
    44401 atattcaccc gtttatcgtc gttatctaaa gcaacataac gtttagtcgt gaaatcattg
    44461 atatcaacat aatcattttt ctttcccaca gcgctttgtt cattgacatc ggttgttcct
    44521 cggatgatcc ctttttcgtc aacgacccgt atttcgatga tatcgctagt cgcactgttt
    44581 tcaataaatc ttttgatatt cgcatttacc tcttcatcat ctgcattatc ttttcgattc
    44641 aattccgggc tgattgttgt tgctaatgat tccaccgttt gattcatatc tttaataaat
    44701 gtattgatcg tggaacgttc taaaccacgt ataaagtagg caccaataat ttcgatcgag
    44761 atcaataaaa tcagaataaa ggtaatagcg atcttaaaat tgactgatcg aaaaaagcgg
    44821 acttttcttt tcatccaatt actcctgttc tgggttacgc aaatagtaac cgacaccacg
    44881 acgtgtcact aaataatttg gatggcttgg attatcttcg atcttttcac gcaaacgacg
    44941 tacagttaca tccactgttc gtacatctcc aaaataatca taaccccaaa ccgtttgtaa
    45001 taaatgttct cttgtcatca cttgtccaag gtgcttcgcc aagtaaaaga gtagttcgaa
    45061 ttcacgatgt gttaactcga ttgtttctcc tcgtttcgtc accatgtaag cttctggatg
    45121 gatcgtcaaa tcaccaatcg tcaattccgc aggtgctgcc tcttctggtt ccttcgcagc
    45181 cgtggcaccg cgacgaagat ttgctttgac acgtgcaact aattctcggt tggaaaatgg
    45241 cttggtcacg taatcatcgg cgcctaactc taagcctagg actttatcaa tttcagaatc
    45301 tttggctgtt accataatga tcggcatatc ataagttttg cgtacctcac gagcaacttc
    45361 taagccgtcc attttaggta gcatcaaatc aagtaaaatc aaatcaggtt ctacctcagc
    45421 aactttttcc aaggcttctt ccccatcata tgcagtataa acgtcatagc cttctttggc
    45481 taaattgaat ttgacgatat ccgaaatcgg cttctcatca tcgacaacta aaactttttt
    45541 cataaagagc cacctcttat atattattgt cataccttct cattataccg tatttctgct
    45601 tttgtttcat ctttgcctat aaaacaaaag agaaaattaa aatgaaagtg caaaatactt
    45661 tattattttt tgttctaact tcaataaaaa aggaaaacac tgattttcac ctcagtgttt
    45721 cctttttttg atttatcctt ctaaaacttg tacaccgaca cccaataatc ccggaccagt
    45781 gtggactact aatgccggag aaatctggcc aaagtagatt tggtttgctt gtgggaactc
    45841 tcttttaagt ctcgcttcca tttcaattgc ttcttctttc gcatccccat gggcaacagc
    45901 taagttgaag acctttgcat taccgatttt ttctttgacg taagcaaccg ttttatccaa
    45961 acttttttta cgtcccctag cttttcctac tgtgtaataa atcccttctt cattacatga
    46021 gatgattggg ttaagcttta aagcggttcc tacaatagaa gcaactaaac cgatccgtcc
    46081 acctttttga agatattcca atgtcgcaac attaaagaaa acttttgttc tttcgactaa
    46141 ctcattcaga ttactgatca gttgatccca ttctactcct tcttccagcc attttgctgc
    46201 ttggatcgca gaaaaacctg caccaatacc aatatttttc gtatctagta catatgcttc
    46261 caattcttct tgctgttgtg caattaaacg aaccacatta tatgtcccgc tcaatccact
    46321 ggaaattgtt accgcaagta ccttttcata accgtctttt ttgatttgct caaagatttc
    46381 tgtgatcgac tcaccatccg gcaaagatgt accagggatt tcctctggca aacgttgata
    46441 gatctcttca ggggtaatgt caattttatc tgtatatacc tgatctttat aaataatttt
    46501 caatgggatc atatacatct cgtatttttt tatcaattct tctggaacat ccgtacctga
    46561 atctactaaa attgcgattt tattgttttt catttgtttt ctcctctttt aaatattcgg
    46621 tttctagaac aatcgttttt tccgcaagaa gtttcattgc aaacgataaa gttgccgctc
    46681 ttacagcaat atattctaca ggggttgcct ggtcgaattt ttgcggatga gatttacctt
    46741 gtgctaattg gctgaccatc cacacagctg cttcttgttc atcgcaaaat agattatagg
    46801 cttcacgaat tcctactgtt tttccttgga acaatatccc ttctttgatt tcaggaatag
    46861 tcaatacttg tttcaacgta gtgattgcaa gtaaaaatgc tacgtgttct ttgttatatc
    46921 tttttttaac tggtgctggt atcaaaccta gtttaacgta attgtttacc atagaagagg
    46981 taagcaatgg atgcttttct ccttgtatca caggtgaaag ataacgatca actaacgttc
    47041 tgacttggtc catataaagt tctaagtcag ggagttcttc ccagtgaggc agacggaatg
    47101 tttctagttc cttcccccac tccattaatt gctgacgcat tttttccatc ataaccgctc
    47161 cttttttcat ttcataaaca tgatatcaga cttatgataa acattcaatc tagttgcttt
    47221 aactaggtga tttgattagc gggattccat gaaaacaaaa aacaagaagg ctaagccttc
    47281 ttgtctttta tcgttaagaa caggagaccc gccggggaga cggcgggaaa ggagtaaaaa
    47341 atgaaaaaag tgttagggtt tgttctcttg caagaacttt gtttgttggt atgcttatat
    47401 aatagcgagc aaatgtgaat aatctatgaa tgatttccta taaggaagta aagatttttc
    47461 tgattttctt taaatgtagt cttgcatttt aaaattctta gtgctactat tattaagtac
    47521 tttttgtacg ggccgttagc tcagttggta gagcagctga ctcttaatca gcgggtcgcg
    47581 ggttcgagcc cctcacggcc cattgggtgc caaacccacg gaacaggtaa atgttggtgc
    47641 gaaagcattt cgaaaacatt tacgctgttc tttttttatt tttcaaaata actagggctt
    47701 tttattttta actcaaagtc cactgtagcc ctttggactt tgagttaaaa aagaagcata
    47761 attatgaaca gtaaagtttc taatataaca ataattcgag aagctttgca aaatcatgct
    47821 gccaatgaac tctctaaaca tataggatta aatctaagta ctataaaagg ttaaaaagtg
    47881 aagaatattt aatagaaaaa ctaaatttac ttgctgcttt ccccttttcc attaatacaa
    47941 caatcagaga gtgtcttttt ttcgaaaaaa ttagaataaa taaaagacac gtagtttttt
    48001 tatctacatg tcttccatcg actacaagtt atcatttctc aaaacgtatt tagcttttaa
    48061 ttgattaatc gtttcacgtt gtctacacat tgcctaatta agattttctt attagaagca
    48121 tcaatcccgc aataatacct aaaactccag gaattaccca caataatgtt aaaagaaaaa
    48181 atggaataat agataaaatg agtaaactaa taccatagac cactgttttt tgattttttc
    48241 ttactaaaaa acctattatg aaaatcatta tcgaaaaaac tagggtaatt cctccaaata
    48301 aaacaacatt cccgcttaga ttgactaaca tatctatatc cttactggtt agttctgtag
    48361 tatttaaagt agttgaatca atagaagata gaaacatacc acccatcaat gctaaaagac
    48421 tacataaaat ccctgttatt ccactaatta aaatcaaaat aaactctgtt tttctcttat
    48481 tcatttaatt ctctccagat taagtgtttt agaattagaa attttttgac catagttaat
    48541 agcttctaat gaacgaataa caaccactga cgtgttagaa aaataatccc caatatgttg
    48601 tttcttgggc gtgaaagcag ccaccaaata cccaatttga aatacaaaat tttttaatat
    48661 aaacctacat atacactcac gaataaagat agtttgccaa cttagctctt tttcctcaaa
    48721 gctcactact cttatcccaa aaatcatttt cccaattgtt tgtcctgaat tcagcttagt
    48781 caaaagtaca aaataggtta aataaatagc taatgacgct ataccataaa aagtaataat
    48841 ggatttgtca gattccagtc ccatgatttt gtaaaagaga cctatagtag catgggtgat
    48901 gatgctaata caaattaaat caataagaaa agcaaaaaat cgtaaccaaa atcccgcaaa
    48961 tacataatta ggcaaagttt caatgccact cttcttattc attttatagc cctccatagt
    49021 aatacattgg ttttggagta tcagaaaatc caagtttttc aaaaactgaa attacacgat
    49081 ctgatgtagt tgcctttagc ccttgccatt cagcaatctt attacctaac caagaagaag
    49141 caaagccagt tgtgtcattt ttatattcta taaccgtcgc atcagttaat ttcttctctt
    49201 tttttaggga atctaatgca tcttcagagt aaccaatttg atccaccaac ccattttcta
    49261 ctgcttggtt accatcataa attcttccat ccgcaagttc tttcactgca ttagtatcca
    49321 tatttcttcc ttttgctaca acattgacaa aacgatcata agcactcatc acaaattcct
    49381 gtataacttt attctcttcc tctgaaggtt tgtgttgagg tgacatcata tcttttaatg
    49441 caccactttt atacgtcgca tcagttacgc caagtttttc taataagcca gaataattta
    49501 aaccagaaat aatgacacca attgatccag ttgttgtttc ttccgtagca aatattttat
    49561 ctgaaccagc agaaatgtag taccctccac ttgccgctgt atttttaaat gcagtataga
    49621 taggaatatc tagcttttta atttttgcca tttctttagc aatctcagca ctttcatata
    49681 ttcctccccc aggtgaattt acttctaata acactccctt aaccgctttg tcttcttgaa
    49741 ttttctttag ctgtgttaaa aaattctgat ggttatattg ttccctacta aaaagattgc
    49801 tttcacctgt atctgcaata actccattga cggatagttt gacaatcttt ttatttgatg
    49861 cgccttcttc taacgtttct tctgtgattt cgttagatcc gtaaaaaatc ttatttattc
    49921 ctgttagggt tgtatttgat gcttcctctt tatcttttcc tggaattagc gcgacgataa
    49981 tggatagtaa caataatccg cacgctactc ccacagcaat ccatcttctt ttattcatat
    50041 aaactcctcc tatattctta gtaaaaataa ttatttttta tttagaaaca catcgacaat
    50101 ttgttgccaa tatgtttttt ctcctacaat taaagacaac tctccagtta atccataacg
    50161 aaacggaaac gattgagttg tttttaacgt tcctttcact gcataaaaaa cacctttctc
    50221 agtggcagta gaattttctg aaatttcatc taaagttccg tcgatcgtaa tcggagcccc
    50281 acctttttga tccaatttaa aatgaacctt catcccttct ttaatatagg tactttcatt
    50341 cgcaggtaaa agtgctgtaa atttcatttt tgatttttcg tggatcggat aaatttctgc
    50401 aagcactgtt cctttggcta gttctgcttg tccctttgtt tcttcattta aatgaaccgt
    50461 tccagataca ggagctttta gtattccttt tgaaagttga tcgtccaaac ttttgataac
    50521 tgactggttc ttctcttgtt tttcatttag ctctgtaact gtttgtttta cagttgttat
    50581 tgcttgttct agctcttgct ttcctttttc cgtttgacta attctgtcat tctcagaagt
    50641 agttggttta actaatttag ctttttctga ctgatactgt tctacttctt tttttagttg
    50701 ctcaatttgt tcatttatgc ttgctgaaat tgtcagtttc acttgatttt tttgatcatc
    50761 agaaacatta tttagttgtt cttgccaatt ctcatagttt gccatcattt cttttgaaaa
    50821 atcttttaat gattgattat tactccaagc tacctgtact tgttgccatt cttgtaattt
    50881 actatctgaa ctctcaattt gtttttctag ttgtcttttt gtcttttcgt atacttctaa
    50941 ttgcttttga tcatttaatt cactttgctt caaagcgtag cggagtgatt ctttttctga
    51001 caacatgctc tttaattgat tactgtagcc aaaactgtca tccgttgaaa acaaattttt
    51061 tcctttactt atactttcaa taaatagctg agccatcttt atttgttgag taatacgttg
    51121 gttttcttgt tctatctgag ccttttcatt ctctattaaa gaacaatcca atctaaccaa
    51181 cacatcgtcc ttatgaacaa attgattttc ttttaggaag ttttccatta ttttagcttc
    51241 taatggcact tgtaacttct ctattttttc ggggttcaat tgagccgttg tccgaataac
    51301 aacctctttt ttagcaaagg ttaaaaacaa tccaataata cataaaaaaa gaattacagg
    51361 gtataacacc caacggtaga agctatgatg ttgtttagta tagatactag aatgttcgaa
    51421 ccaattatta ttctgcataa attgtttccc tctcctagat ttcccataat ttttgataga
    51481 taccatcggc aaaacgaagt tcatcgtgcg ttcctttttc taccaaatgc ccttcatgga
    51541 gaactaaaat ttgatcacat gcttttgcta ttggtaaatg atgcgcaata aaaataatcg
    51601 ttttttcttt taaattgagg agataattca taattgcatg ttctaacaag gtatctaatc
    51661 cactagttgc ttcatctaaa attaatatgt ccggattttt taataaagcc cgtgctaagg
    51721 ctaatcgttg tcgttgccct cctgaaagat tatttccacc ttcttccaac aatgtctcgt
    51781 aacgtaatgg ctgttgatta ataaattcat ttagttggac tatctcacaa acttctatta
    51841 tttgttcgaa acttggtggg ctatctaaac cgaatataag attctcaatg attgttccac
    51901 taaagaaaaa tgactcttga ggcaaatatg tcactcgatc gcgtaatatc tgaaaaggaa
    51961 tgtctaagtg attaatgttt ccataatgga tcgatccttc tgatgcttca taaaaacgaa
    52021 caaataactt tgcaagagtt gattttccag aaccactaac tccaactata gccgttttac
    52081 tgtgcgaagg aattgtacat gaaatttcgt tcaacgttgg ggccttcatg ctatacgaga
    52141 atgaaacgtt ttttacagtg atatcttgtt gaaataattg ttcagaaagc atatattcaa
    52201 aagactggtt cgatttttct gaatcaatcg cgaaaatttc atttaaacga ttatttgcaa
    52261 cttgagccgt ttgcattttc acttgtaaat taataatgtt ttgtaaagga tctgtcaaaa
    52321 atactagtaa agcattgtat gtgataagtt gacctagact aattctatta tccataacaa
    52381 aataagaacc tagccataaa attaatgcac tgctaattaa ttgaatcaaa tgtttaattc
    52441 cttgttgaat attatcaagc gtaaccgtac gaaatgattt tttcattaat ttaacaaatt
    52501 catggtctat acggttatat actttctctt caccgctata tgctttaatt gtttctattc
    52561 ctttaagact ttctatgata ctagaattta cagctgcacc ggctgccatt tcttccctgt
    52621 tcgatttttc atagcttttc acgaaagcga atattgcaac tacataaaaa ggcaaagagc
    52681 atagagtgat taaaaacaac gtactattct gaatagctaa cgtaacccca acaagcaaca
    52741 ccataccaat atctaaaaat attgataacg tagcacttgc taaagcatca ataatcttac
    52801 tggcatcaag aaaacgagaa ataatttccc ctgattttct cgtagcaaaa aaattcattg
    52861 gtaaagttaa cacatgtttg aaatactgaa gcataattgc catactcatt cgctgtccta
    52921 aaataataag aagataacta cgactatatt caaacaatac ccgaaataaa tagacagcga
    52981 tcaagccaat ggaaattata tttaaagttg aacgtgcttg attagggatg aaaaaatcaa
    53041 gaataccttg aaaataatat gagcttccta tgccaaaaaa cgtaatgaaa atcgatgcta
    53101 aaacgatgtg gaatactaat cctttttgtt tccaaattat aggcaaaaaa gaacttaatc
    53161 cagctacttt ttctttaata ggaatataag tttctctagg cataggtaat aaaagaatac
    53221 ctgtccattc tttttgaaac gcttcaatcg tttttctatt ttttccatta gcgggatctg
    53281 caatccatag tacgtttccc ttcgttttat aaattacaac gtagtgcata tagctgttat
    53341 caaccaatat atgtgcaatt aatggtaaga taagatctct ttctttccag atctcactat
    53401 ctgcttgaat agccatacaa tcaaaagaca atttttcaaa tgtctttttc aatccaaatg
    53461 ctgaagtacc ctctaaatca gtcccagata ggtcccgtaa tttatgaatc gggatatcag
    53521 ttttaaaata ttttaaaatc atcgaaaggc aagcaacacc acaatctttt tcatcttgtt
    53581 gctgcacaaa aggaaatgat tttatcactt atctaacaca actccttcta aaaaattttt
    53641 atagaaaaat tctcaatata gctttaatac agtgaaaata ttgtctcata attccacaaa
    53701 aactttgaaa acagttcttg aacactaact ttttcattcc tgaatcaaat ttattttgga
    53761 aattttacta ttcaagaata aacttgataa gtttcaggaa gatttttact gttaatcaaa
    53821 tacgtatgtt acagtagcac cgtaaaaaat attatttaaa gaagcgtgtt taaacatgca
    53881 aaatgtaaag gaactaagta ttgtggaaat gcacaaactg ttggtggagg caataatgct
    53941 tggggaaaat tggggcaagt tgtaggtgga cttaccacag gggcagtagg tggtgcaggt
    54001 ttaggaacag caatttgcgg accagcttgc ggtgttgttg gagggttata tggagctgtc
    54061 gctggaggtg ctgcagctgg ctgggatgct cgaaaaaaat aaaaaaggag aataagtagt
    54121 atgcatatta aaaacacaaa aacaacattt atattatctt cagaagagtt gaaaaatatt
    54181 caaggtggga gtgccgtggg agttttaggc actacttttt ctggggcaac agcaggagta
    54241 aaactttgtt cagccggagg accttatgca atagctgctt gtggtgtagg tggagcttta
    54301 ttaggtgctg gattcagcat gtggaccggt gcgtaaacaa ttaaaaaata tcaaacctca
    54361 agattggttt gtgtcgatta cgaacatagg tatgttcttt atcttttcaa aagtttggaa
    54421 tggacagaac tttttactaa agcttcttgt gctagttttt ataattatat taaattttta
    54481 tcttttatta aagtcagata aaaattcttt ggaaatgtaa tggtagagat atttttacaa
    54541 aaggacttgc agcgctgtag ttggtgctgc aggaggacct atttcggccg ttacaggagg
    54601 tattggtaat gtacttgtcg gatgtgcatt aaagcctgtt cctgcaagtt aaatttataa
    54661 aaacaggaaa gtaattatct caaatcctat caagtacaat cataggattg cacttgacag
    54721 gacaaatttt ttaactaaaa aatgaaaaac actttctaaa aattagtaga atttgccaaa
    54781 tctttcgcat cttccaccat acatttaatt ttaattcccc tttatggtac ttaataaaaa
    54841 caagaaaaga actattcatt tggccctttt ttggttccag accatagaaa tattcctcca
    54901 gtaactaata ttgctgttat gcacgtaact aataacagct ataaaaattg gtacaacttt
    54961 aataggattc agaaaaattt gcactagtga aggaatctat tttattagaa aaatgcaatc
    55021 tccctaaaga aaaaaccact aaacagtgct ttctttcaac ttctccttaa ttttttgtgc
    55081 tttccctaaa gtaaagtttc tggccaactc tgaatcaaaa tatagtgaac gttttttaaa
    55141 atctatttct ttaacgttaa agatatttac taaacacgct cgactaattc ttgttaacat
    55201 aggatatttc ttttcaagct cactaattct tccataaaat tcgtactgcc catctttagt
    55261 acacaatgat aatctatgtg gtaattttga agcttctaca aaataaatgt catttatatc
    55321 aaaagtaaat gtttgagaac ctatggaaaa aacaaatgct tgattcgtaa cctgttttgt
    55381 tgcatctatc ctttcttgcg ctaataacaa taactcaaca atctcgtttc gatactcatc
    55441 tagtgtttga tcttttgtta caaatcctaa cgtttcaact cttcttttta ttgttacggg
    55501 taacatctca tcatgagtag ttgtaaaaat gatttttgct tgaacatcat attttctgat
    55561 tacttctgct aattctatac cgttaacttc atgatttaaa tcaatatcta agaaatatat
    55621 tccttgcttt ggtcggaatt gtttgaggta ttttttaact tctaacggac tttgtgtttt
    55681 caatactatc ttaaatcctg aataattcat acttttaatt aaaaccatgt gaaactgcct
    55741 cacggcagct tacaattact ttttcatctt cacccgttaa gtgatgaaaa aagaacccct
    55801 ttctgctatg gttaaagtga ctaaaccaac caaaagaaag gagttctttt catgactagc
    55861 ttacacaaaa accaagtaaa attcaattca aatatcacta tttctcatac aggtggtcaa
    55921 ttatcgagtg attcgggtct cgtcctagtg aaagagctga tgaacatctt tggtttttct
    55981 gaactggcta agcaacacat tcacattgaa gacaaacgag cgtattttac tcatgacaac
    56041 ttatccatac ttgagcagtt cattatgcaa ttaattgctg gttactcagc tgattctgca
    56101 gctaatctcc tgagacaaga tcctgtgttt aaagctgttt tagataggaa agaactcgct
    56161 tctcaatctt cactttcccg atttttagat cgactatctg aggagaatat ccatgaactt
    56221 caagctttga accaagaact tatcgataaa gcacgcctca tccgtaatga tacggaatta
    56281 atcattgatt tagattcgac ccattcagat acatttggtc accaagaaca aacgaattat
    56341 aatacacact accaaaccta tggttaccat ccattggtag cttttgacgg attgactggt
    56401 gacttcttaa aagctgaact acgttcagga aatcaatata catcaaaagg cgtaaaggag
    56461 tttctcacac ctttattaga gcactataat cactctctac caaacactga catcttggtt
    56521 cgtggagaca gcgggttcgc tacacctggt gtgtatgatt cgtgtgaatc aaaaaagagt
    56581 cattatgtta ttcgactgaa gaataatcgt agactaggtc aaatagctga gaaatcagta
    56641 ctttatggcg ataatcaaaa gtgggaagaa cgagaagttc agtacttctc gaccacttat
    56701 caagcacaat cctggtcaca aagtcgtcgc gtgtgtatac gctcaacacg tgaagcgggc
    56761 gaactactct ttcgacatga atttatcgtg acgaatctat cagaaaatgt ttctcctgat
    56821 accatctttt ctatctatgc caaacgtggc acaatggaga acttcattaa agaagcgaaa
    56881 gctggctttt actttgacaa aacagacagt cctcgctttt tggagaatca tgtccgaatg
    56941 atgctcagct tgatagctta caacttagtc aacttcttaa gaacgattgg ctttgaagaa
    57001 gtccaaaagg gaatgaccat tcattctatt cgattgaagt ttctgaaagt tgctgggaaa
    57061 ttagtccaaa cgggtagacg agtctatctc aaattatcta gttatcatgt gtatcagaat
    57121 gaattctaca gggtctttgc tcgcctgagg cgagccagtc aatggatcta atccaacaat
    57181 ctaacgattt ttttactggg aagttatcca gggagaagta tgctcaaaat gccttacacc
    57241 caaaatttat tcctcaatat ttttgaacag tgaatgtttg aagtaacttt ttagtcaaaa
    57301 tcagttgata gggatattag tgatacatat ttcaataaaa tgtgccaaag aat
//
LOCUS       DACEKG010000006        56296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEKG010000006 DACEKG010000000
VERSION     DACEKG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.006"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..189
                     /locus_tag="IW664_000305"
     CDS             <1..189
                     /locus_tag="IW664_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0847698.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IW664_000306"
     CDS             complement(1055..1585)
                     /locus_tag="IW664_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ0847699.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IW664_000307"
     CDS             complement(1582..2199)
                     /locus_tag="IW664_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0847700.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IW664_000308"
     CDS             complement(2781..3002)
                     /locus_tag="IW664_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ0847701.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IW664_000309"
     CDS             complement(3180..4712)
                     /locus_tag="IW664_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0847702.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IW664_000310"
     CDS             complement(4848..5933)
                     /locus_tag="IW664_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0847703.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IW664_000311"
     CDS             complement(5948..6667)
                     /locus_tag="IW664_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0847704.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IW664_000312"
     CDS             complement(6664..7974)
                     /locus_tag="IW664_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0847705.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IW664_000313"
     CDS             complement(8001..8591)
                     /locus_tag="IW664_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ0847706.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IW664_000314"
     CDS             complement(8909..9970)
                     /locus_tag="IW664_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0847707.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IW664_000315"
     CDS             complement(10538..11605)
                     /locus_tag="IW664_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0847708.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IW664_000316"
     CDS             complement(11625..12104)
                     /locus_tag="IW664_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ0847709.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IW664_000317"
     CDS             complement(12121..12582)
                     /locus_tag="IW664_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0847710.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IW664_000318"
     CDS             complement(12596..13846)
                     /locus_tag="IW664_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ0847711.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IW664_000319"
     CDS             complement(14224..15675)
                     /locus_tag="IW664_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0847712.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IW664_000320"
     CDS             complement(15996..16760)
                     /locus_tag="IW664_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0847713.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IW664_000321"
     CDS             complement(16788..17486)
                     /locus_tag="IW664_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0847714.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IW664_000322"
     CDS             complement(17498..18277)
                     /locus_tag="IW664_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0847715.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IW664_000323"
     CDS             complement(18293..19237)
                     /locus_tag="IW664_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0847716.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IW664_000324"
     CDS             complement(19283..20062)
                     /locus_tag="IW664_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847717.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20442..22517)
                     /locus_tag="IW664_000325"
     CDS             complement(20442..22517)
                     /locus_tag="IW664_000325"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0847718.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IW664_000326"
     CDS             complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IW664_000326"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0847719.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IW664_000327"
     CDS             complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IW664_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0847720.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24783..25682)
                     /locus_tag="IW664_000328"
     CDS             complement(24783..25682)
                     /locus_tag="IW664_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0847721.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25697..26098)
                     /locus_tag="IW664_000329"
     CDS             complement(25697..26098)
                     /locus_tag="IW664_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0847722.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IW664_000330"
     CDS             complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IW664_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0847723.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26581..28773)
                     /locus_tag="IW664_000331"
     CDS             complement(26581..28773)
                     /locus_tag="IW664_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0847724.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28797..29768)
                     /locus_tag="IW664_000332"
     CDS             complement(28797..29768)
                     /locus_tag="IW664_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0847725.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30473..31852
                     /gene="brnQ"
                     /locus_tag="IW664_000333"
     CDS             30473..31852
                     /gene="brnQ"
                     /locus_tag="IW664_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0847726.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(32008..32454)
                     /locus_tag="IW664_000334"
     CDS             complement(32008..32454)
                     /locus_tag="IW664_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0847727.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32481..32657)
                     /locus_tag="IW664_000335"
     CDS             complement(32481..32657)
                     /locus_tag="IW664_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0847728.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32819..33247)
                     /locus_tag="IW664_000336"
     CDS             complement(32819..33247)
                     /locus_tag="IW664_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0847729.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33339..34199
                     /locus_tag="IW664_000337"
     CDS             33339..34199
                     /locus_tag="IW664_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0847730.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34293..34586)
                     /locus_tag="IW664_000338"
     CDS             complement(34293..34586)
                     /locus_tag="IW664_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0847731.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(34659..36386)
                     /locus_tag="IW664_000339"
     CDS             complement(34659..36386)
                     /locus_tag="IW664_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0847732.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="IW664_000340"
     CDS             complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="IW664_000340"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0847733.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37670..39103)
                     /gene="ascB"
                     /locus_tag="IW664_000341"
     CDS             complement(37670..39103)
                     /gene="ascB"
                     /locus_tag="IW664_000341"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0847734.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(39103..40506)
                     /locus_tag="IW664_000342"
     CDS             complement(39103..40506)
                     /locus_tag="IW664_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0847735.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40736..41638
                     /locus_tag="IW664_000343"
     CDS             40736..41638
                     /locus_tag="IW664_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0847736.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41679..43565)
                     /locus_tag="IW664_000344"
     CDS             complement(41679..43565)
                     /locus_tag="IW664_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0847737.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43662..44514)
                     /locus_tag="IW664_000345"
                     /pseudo
     CDS             complement(43662..44514)
                     /locus_tag="IW664_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(44849..46633)
                     /locus_tag="IW664_000346"
     CDS             complement(44849..46633)
                     /locus_tag="IW664_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847738.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46633..48384)
                     /locus_tag="IW664_000347"
     CDS             complement(46633..48384)
                     /locus_tag="IW664_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0847739.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48526..48750)
                     /locus_tag="IW664_000348"
     CDS             complement(48526..48750)
                     /locus_tag="IW664_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0847740.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            49001..50587
                     /locus_tag="IW664_000349"
     CDS             49001..50587
                     /locus_tag="IW664_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0847741.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(50653..52428)
                     /locus_tag="IW664_000350"
     CDS             complement(50653..52428)
                     /locus_tag="IW664_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0847742.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(52639..53541)
                     /locus_tag="IW664_000351"
     CDS             complement(52639..53541)
                     /locus_tag="IW664_000351"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0847743.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            53820..56165
                     /locus_tag="IW664_000352"
     CDS             53820..56165
                     /locus_tag="IW664_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0847744.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        16684 a        11715 c         9117 g        18780 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    20401 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    20461 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    20521 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    20581 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    20641 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    20701 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    20761 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    20821 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    20881 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    20941 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    21001 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    21061 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    21121 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    21181 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    21241 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    21301 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    21361 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    21421 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    21481 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    21541 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    21601 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    21661 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    21721 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    21781 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    21841 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    21901 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    21961 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    22021 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    22081 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    22141 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    22201 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    22261 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    22321 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    22381 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    22441 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    22501 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    22561 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    22621 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    22681 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    22741 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    22801 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    22861 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    22921 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    22981 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    23041 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    23101 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    23161 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    23221 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    23281 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    23341 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    23401 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    23461 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    23521 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    23581 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    23641 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    23701 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    23761 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    23821 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    23881 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    23941 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    24001 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    24061 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    24121 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    24181 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    24241 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    24301 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    24361 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    24421 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
    24481 ggagcaaagc cggagctaag ggctgatttt gccgatggat ctctttgata taaaacatca
    24541 ttttgccaga agattctgta aagattttga ctaatttatc tttttctttg taattacggc
    24601 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    24661 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    24721 tctcaacacg cggattttta ttcagtttat ttctatacct aatttacaag aagctcagaa
    24781 tatcagtatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    24841 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    24901 tcttttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    24961 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    25021 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    25081 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    25141 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    25201 cgttcgacgt tgtttccttc tgtagcagag attggaatca cttgtgcaaa aggcatctgc
    25261 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttgtc gattttattg
    25321 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    25381 cgaccgcgtt tttgatcagc actaatcata aataagaccg cgtccacttc tttcaacgca
    25441 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    25501 gtatcaataa aaatcaactg agcttctggt gtagtgtata ctccttggat tttatttcta
    25561 gttgtctgtg ccttgtcact catgatggca attttttgac ccacgattcg attcaataat
    25621 gtggatttcc ctacatttgg gcggccgacg attgcgacaa accctgattt atgttctggc
    25681 atatatgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    25741 acccaacgat gacggtaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    25801 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    25861 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    25921 ctatccgatc aagttggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    25981 gtttcctcat gttgcgttct tcctcaaaaa cagttttgac tccttggacg gcaaattcca
    26041 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    26101 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    26161 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    26221 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    26281 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    26341 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    26401 acttggattg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    26461 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    26521 tggtttgttt catccatgaa ggtaatatcc attatacatc gcttctttct attcttcttg
    26581 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    26641 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    26701 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    26761 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgcccgta ctgccgcttc
    26821 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    26881 acggaattgc tcttccgtaa cttccgggtt gcgttccttt gccttaacgt agaaataacg
    26941 catcagcgtg gttccatgat gctgtctaca aatatcgatc accatttgcg gcatattata
    27001 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    27061 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    27121 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    27181 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    27241 cgtacctggt gcttcttcca gcaactgttt caataaagga tgattgggat tgctgagttc
    27301 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    27361 cgtcagcaag aatgaaaaca gtgcacctgt gaatccgcag actagcgtta accaagtcgt
    27421 tgcatcccca aagctcattc cttggtagat aatcaaaatg acatccatca tcacaggaaa
    27481 aataatgacc cacatagcag cggataaccc ttgttctgag atccgtttgc gtctgacgat
    27541 cgttgccatg agaccagaaa atacatagga catcaaaata actgtcagaa agttcgtgcc
    27601 aatcgaatca taaaaaatga aaagtgcgga tactgcttga aaaataccag ccatgatccc
    27661 tgctcttcga ttcaagaaaa agcttaaaac aagtggagca aaagccgcgg ggtaaaacag
    27721 cggaatataa gcagcttgtt ctgtttggaa gagttggaaa aatttcatta gtacgacgct
    27781 tactgacatt gccgtcacgt agaaaagtac gtattttgtt ctttctcctg cattttggaa
    27841 ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat
    27901 gagtgggaaa atagaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc
    27961 tgaagcatca atttgatttc cttcgcgaac aataatttct ccttggaaga tcatcactgg
    28021 ttgtacagct tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc
    28081 attaactacg atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac
    28141 attcaaatat tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg
    28201 aacttgttta ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat
    28261 ttccgtttca gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa
    28321 cgcaacgttt tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc
    28381 tgtagtaggt ttcgggatat tttcatcttc cttcgcctta tctaccttgc cattataaga
    28441 tttgttgatg gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt
    28501 ctggtcatct gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag
    28561 ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc
    28621 ttctgctact tggccttctc tgaaatcaat ggccttttgt ttgacactac taaatgtaac
    28681 gccgcagaca atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt
    28741 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    28801 tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    28861 acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt taatgttcgt
    28921 tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact tgtatcccca
    28981 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
    29041 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg
    29101 taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt atgatccaat
    29161 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc
    29221 agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg cgtcaaaatg
    29281 attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt
    29341 ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg
    29401 tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    29461 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc
    29521 gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
    29581 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
    29641 cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca
    29701 cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct
    29761 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    29821 gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca
    29881 agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat aaaaagaagc
    29941 tgagaatgta agttatatga aagtggagaa caaaatattt cgtttcattc aacaagtatt
    30001 ctctaggtat ttttcctgtt tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt
    30061 tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag cgtcgaggaa
    30121 aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa atgacaataa
    30181 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
    30241 tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt
    30301 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    30361 ctttttttta ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt
    30421 gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga
    30481 aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt
    30541 cttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg
    30601 ggtcagtgct ggcgtaggat ttgctatctc aggtgctctt ttcccattac tagctatctt
    30661 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    30721 gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc
    30781 acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa
    30841 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    30901 ccatccaaca cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt
    30961 attagcaatc atttttgtcg tttctttatt ccatccaatg ggaaatctaa atcaatctgc
    31021 tgcagatact tatcaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt
    31081 tgatgcagta gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg
    31141 tttcaaagat aaaaaagtca cagaattaac ggcaaaagct ggtagcctga gcgttgcgct
    31201 agaagtcatg atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc
    31261 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    31321 cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact
    31381 cgttgtttct tttgcgcaag atttccataa actgttccct aaagtcagtt atatgacatg
    31441 gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttag acaatatcat
    31501 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgatcttgct
    31561 ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt
    31621 tgctgctgtt cctgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca
    31681 aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg
    31741 acttggctgg atggtcccaa cgttacttgg ttacgctggc agcctactgt tttattacgc
    31801 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccaaatca
    31861 aacacaattt aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta
    31921 ttctttcgtc tagctatttt ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa
    31981 tgattgtccg cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt
    32041 tacttggttt ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc
    32101 aaattctttc attgatgaag caccagtttg tgtgattgct tcttgaacaa gttgtcgaat
    32161 ttcttcgtcg gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac
    32221 tttttcagct aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg
    32281 tttcatttcg cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt
    32341 aatctgctcg ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc
    32401 tttcgctttc atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc
    32461 aactccaatt catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc
    32521 ttgcgtctta cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc
    32581 gcttttgaca cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga
    32641 acgactgttt ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt
    32701 attgaaatat atctcaaaaa acaagactgt cctgtaaata ttatatctaa tgctgttttg
    32761 accgtcaagg aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt
    32821 attgacaatt ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac
    32881 cgagctgttc ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca
    32941 ccgtacagat aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact
    33001 tcatctctcc atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat
    33061 ttcgataaat cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt
    33121 ctttagcgga tacgtagcga ttgcgcttca ctaaataagt taacaatgct tctctttttt
    33181 tagtatattt aaaaccgtta tttttcaatg tttctagtga ttgttcaatc aatgtctgtt
    33241 ttttcatgac ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt
    33301 ataataaaca taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa
    33361 tcattacttt ttttgtcatc tctgactctg ctggagaaac tgcgacaaaa ttagcgcaag
    33421 ccactatggc ccaatatcct accgttgagt tcaatttatt ccgcagaaca tttgtgacag
    33481 atgagaaaac attgatgcag gcactgcaag atgcactaaa agaaaaagcc cttgtcctac
    33541 acacattgat caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat
    33601 tatttagttt tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca
    33661 agcccattcg gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatca
    33721 aagcaatgga atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag
    33781 cagatgtcgt tttgctaggc gtatccagaa cttccaaaac gcctttgagc ttgtttttag
    33841 ctaacaaaaa tttaaaggtt gccaatctac ctttgatccc acaagcacat attcccaaac
    33901 agctttggga gatcgatcct aaaaaaatcg tgggactgac gaataaccca gatattttaa
    33961 acaatatccg taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattccg
    34021 atatcgaaaa aatacgagct gaactcgatt ttgcgaatga tctttatgaa aaattgggct
    34081 gcgtagtaat caatgtcgct tctttatcta tcgaagaaac agcttcactt attttgaatg
    34141 ctttagacct agaagatcat agttattatg gaactgaaac caccgaagaa acagactaac
    34201 agtttttctt gtctttttct taaaaaaact ggactgtgtc agcgaagaag attcgttgat
    34261 acagtccagt ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagcgtc
    34321 tggttcaaaa tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca
    34381 cgatgggact tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat
    34441 tgttccattt ctactgcttg ggtgattgct gtcacatttt gttccccgtc tttcagatac
    34501 agcagaattt tgagacgagt ggggtcactc aacattttga atagttggct gactttctct
    34561 acttttgttt tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt
    34621 ctaaagttta ccactttttc catcagtctc aagttttttt attcttctgt catttttttg
    34681 ataatcgctt cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca
    34741 ccgatataaa atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt
    34801 tcttccagcg tatacttcaa tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct
    34861 tcacggtctg ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca
    34921 aattctgtcg gtgcttcttc acggaatttt tccatcaaag cagagatttt tgcagagcct
    34981 tcttgtccgc tcattgtcac agaaatcgtt ttttctgcgt agtagccata tgtttcaaaa
    35041 atcgtttgca agccgtcata caatgttttg ccttgttttt tatagtaagc tgctacttct
    35101 gctaataaga gcaatgcttg aatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa
    35161 ccgtaacttt cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga
    35221 attttttcag caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt
    35281 tccgcaattg ctgtaggcaa ttcgctagaa acgattgatt tcaataccgc tgcattttca
    35341 ggtaaagtgc ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag ggctccgatt
    35401 tggtttcctg tcaatacttc ataggagcca tttggcaaac gaacggctgc gcctaaacgg
    35461 tctgcatcag gatccgttgc aatcagtaag tcagcgcctt ctttctcgcc aagtcgcatc
    35521 gcatactcga aagcagagtg ttcttctgga ttcggtgatt tgactgtagt gaaatccggg
    35581 tctgcgacag cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca
    35641 cgttcgccca acatttttcc tgttccgtga agtggtgtgt acacaagctt caaatcttta
    35701 cccattgttt ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg
    35761 gcatccactt cttctccgat gatcgtgatc aatccgctat gttcagcttc gtcgtcagat
    35821 aatacttcaa cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg
    35881 tctgcaggag gcatttgtcc cccatcttct ccgtagactt tatatccgtt gtaagctgct
    35941 ggattgtggg atgcagttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa
    36001 gaaagctctg gtgttggacg taaactttcg aatacataag aagggatatt gtgatgtgct
    36061 aatgttttgg ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata
    36121 gcgacacctc ggcgttttgt ttcttcacct tgtgtatcca tgaaacgtgc aagtccttct
    36181 gttgcttgtc ttaccgtata gatgttcatt cgattgatcc caggtcctaa gattccccgc
    36241 atgcctgcag taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct
    36301 tcacccagat catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat
    36361 tgttcataca cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta
    36421 ataagtatag cattcactta ttagatatga aagtatgagg accaaaaaac gcttaaatca
    36481 agaaaagatt cttttatcaa gcacggttac aagtattcgc ataaatcgga ttcagtagat
    36541 ttcacgcaaa aaaaagagta aaagaacaat tgaaaccgtt ctttcactct ttttaaactt
    36601 atgcagttgt ttcttctaaa gattccaagt atttatacac ttgttgtcca gcaatcccac
    36661 catcaccaac agcagtagtg atttgtcgca aatctttttc acgaacatct ccgatggcaa
    36721 aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg aatccagccg gcttcgttcg
    36781 tcaacccagc ttctctaaat ggtgcagtta atggatccaa tcctacatag atgaatactc
    36841 cgtctgctgc caaatcaaac tcattgccag ttttgacatt acgtgctttc acacctgtca
    36901 ctaccatttc atttccaacg atttcgtcaa cgactgtatc ccacataaag gaaatttttt
    36961 cattcgcaaa tgcacggtct tggatgatct tttgtgcacg taattcatct ctacggtgga
    37021 cgatgactac ttcagaagca aattgtgtca aatagatggc ttcttctact gctgagtctc
    37081 ctcctccgat cactactaga cgtttgttac ggaaaaatgc accatcacaa actgcacagt
    37141 aagaaacgcc acgtccagca aattcttcct cgccaggtac gcctaattta cggtggatac
    37201 agcctgttgc gataatgact gtttttcctt cgtaagtttt atcttcacaa ataacttctt
    37261 tgtggctgcc gtgatccttg atatcttgca cgattccata agcattttct gtcccaaatc
    37321 ttgaaacacc ttcatacatc ttataagcta gatcaggacc catgattgaa tcgaaaccag
    37381 gatagttttc gatttctgcc gtattgttca tttggccgcc aggagcgcct tgttcgatca
    37441 tcaatacgga taagtttgaa cgtgaagcat ataaagcagc agtcatccca gcaggccctg
    37501 cgccaataac gattacatca tacatatgta gattctcctt tacttaatca agtaagcata
    37561 ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga atagctttat
    37621 tatactcgaa aaatccgctg aaaaacaata gacttttata aatcattgat caatcgttcg
    37681 aaagattttc tccatttgaa tcaattactt ttttatacca gttgaatgat ttcttcttcg
    37741 aacgggctaa tgtgccattt ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt
    37801 tcttcatctc acctgtacca gctgatacta aatcaataca tccccaagtc gtatagccta
    37861 atagatcgac tccatctaat tctactgcat ctttcattgc ctggatatgt gctgccagat
    37921 agtcgattcg ataatcatct tccacataac cattctcatc gggcgtatcg actgcaccta
    37981 agccgttttc tacaataaat agtggttttt gataacgatc ataaatatca ttcatcgtta
    38041 ttctcaagcc taatggatca atttgccaac cccattcact tgcttccaga tatggatttt
    38101 tgacagaagc aaagatattt cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa
    38161 ctcgagaaga ataataagaa aaggagataa aatcaacagt atgttctttc agcaatgcgg
    38221 catccccagg ttccattttg atctgaatgc catttcgttc catttctttc aatgcataag
    38281 cgggatattc tcctcgtgac tggacatcaa tgaagaaata attttctcga tctgactttc
    38341 tagaagccca aacatcttca ggattgcaag tgtatggata atatgctcct gctgccagca
    38401 tacagcctac tttgttctcc ggatctactt catgagcgat cttcgttgcg atggcacttg
    38461 caaccaactc atgatgtgcc gcttgatatt tgatttgttc ttcattttct ccttcttcaa
    38521 aatacagccc tgcgcccatg aatggtgcat gaaggatcat attgatctca ttgaatgtca
    38581 accaatattt gacaagtcct ttatagcgat tgaagatgac tcggcacaga ttttcataaa
    38641 aaccaacgag ttctctggaa cgccacgcac catattcttt gatcaaatgc atcggacaat
    38701 caaaatgtgt aatcgtcaca agcggctcga tcccgtactt ttgacattct ttaaagacat
    38761 cctcataaaa tttcaaccca gattcatttg gctctttttc atcgcccatt ggaaaaatac
    38821 gactccaagc aatggacatc cgataagtct tgaaccccat ttctgcaaat aaagcaatat
    38881 cttccttata atgatgatac atatcgatcg caatttttgc tggataaaaa tgattttcat
    38941 caaattcaaa cattttcttt tttccagtaa tgacaggaaa acggtcttct ccgattggca
    39001 caacatctac gttcgctaac ccacgtccgc cttcatcata accgccctcg cattgattgg
    39061 ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc catcatactt cctccattac
    39121 atgtgtattg cttgataatt ctcttgccag ttcatcttgg acttcgattc cttctgcttt
    39181 taaccggctg attgtttcct tgaattgcgg taaatactgc tgatgtgcaa taaacaactc
    39241 atccatgatc cgttttgctg tctttcctga tcgtataagc ggattgatcg taaacgcttg
    39301 caaagctgtt ccataatcac cagtcacggc tgcttcgatc gtcagcaatt ccatattctt
    39361 cattacttgc aaccagcctc tttcagcagc aggaagggaa ccaaacgcta catttcgtgc
    39421 gccttgtgcg ccaacatagg cagtgacttc tactacacaa tctgccggca gatcaggaac
    39481 agctccatca ttctttgtag ataccacgat ctgtgtttct ttatttccat agatcgaagc
    39541 aatcgtttca caggcagcat ctgaataata agcaccacct cgtttttgca attgttcagg
    39601 tttatgatcc aattctggat ctttgtataa ttcaaacagc tctgcttctg tttctttcac
    39661 ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca gcatttcttc
    39721 ttcacgatag taatagcggt ggtagccaca agggatcatg ttcatttgtt ttaattgttc
    39781 tttgaaaaac gggatgtcaa agatattttt cggcaatcca ttgtcttcat cgtataaacg
    39841 atcaatcaat tctgccgtgc ggtcatttcc tttattatct gctactttgt gccaatggaa
    39901 gtgatttaga ccggcaaact tgtagatcag ctcttctttt ttcttgccaa gcattgttgg
    39961 ttctgtcatc atcgccatta ctggcacatt gcataatccg atcactttat cccatttgcc
    40021 atatctgata actgcttctg tcaccatccc gcttgggttc gcgaaattaa tcagccaagc
    40081 atttggacat aaacgcttca tgtcttcaac gattgccaaa accactggaa cggtacgaaa
    40141 agctttgaat atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg
    40201 gatccgctcg tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa
    40261 atccgcatct ttcaatgctt cttcccgatc cagagttgca tagattttga cttcatacgg
    40321 agaagcatcc cacatacgct gagacatctg actgacgatc tcaagctttt cttttccttc
    40381 ttctacatct acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc
    40441 ttccatcaac tcgggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt
    40501 tgccatgttc tcactccttt actttttaca cttttattat cttttttgta aacaaaacaa
    40561 acaatagaaa ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt cttctggatt
    40621 tttgtaaata tcttgtcaaa aaatgtcttt attaagctat ataacataaa tccgtttaca
    40681 aatcattttc aaaaaagtta taatttacgt aaaaaaacaa gaagaaggta tctacatgtt
    40741 attacgtgaa aaaatcaaag aaatctcctt ttctccttca gaaaatgaag tgattcagtt
    40801 tctcttaagc gattctccag cacttggcga aatgaccatc caagagattg ctgagcgatg
    40861 ttatgtccac ccgtccacac tcatccgtgt agcaaaaaaa atgggttttg atggctgggt
    40921 cagttttcgt aatgcttttt tagctgaaac agcctatctt gaagggaatt ttcaagacgt
    40981 agatgcgaat cttccttttt ccaccacaga cggtattatg acgatcgcaa ataaaatggc
    41041 ttctttagaa cagatgacga ttcaagatac actttctctc attcatcatg accaactgca
    41101 aaaagccaaa caactaatgc ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa
    41161 tactttgatt tctcaagatt tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat
    41221 tgtcaccact tttggtgaat cgttctatga agcgtacaat tgcacaagtg atatgtgtgg
    41281 gattctgatt tcttatacgg gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa
    41341 acaaaatcat gtgccaattt tagctttgac cagtatcggt gattcaacgc tatcttcaat
    41401 gagtacagcc gctttgcgac ttacgacacg cgaacgactg tattcgaaaa ttggtaattt
    41461 tacgatcaat acatcgattt gttatcttct agacgtgtta tatagttgtg tttttgcaga
    41521 aaattatcaa cagaatttag tccatctaat ttctgtagga caaaaatttg ataaacgccc
    41581 tatcagctca gccatcatga aagagccttt caattttgaa aataaagaag tggactaaaa
    41641 agaatagtta agcctagtcc attctttcac ctaaccgttc aggtcagtgc ggtgataagt
    41701 gcatcgtctg gcccgaccgt ttccccttga ttttccaaaa tatccaaata atccatgtaa
    41761 ttgatgacaa tcacaggtgt ttctaaactg tatccttcct cttcgatttt ctttttgtcg
    41821 aagcgaagca acagatcgcc ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa
    41881 tatttccctt ccaactgaac agtatccaat ccgacatgaa tcagcaattc cagacctgct
    41941 tcagaaacca tcccgattgc atgtttcgtc ggaaacaggg acatgactgt tccgtcaaaa
    42001 ggggcacgta cttctccttt ttcagggtag atcaagattc ctcgacctag tgttccttgt
    42061 gcaaaagctg catcctttgc tgcattcagt ggagcgattt gtccttgtat cggtgttttt
    42121 acaatttctt tacctgatct tttctgtcta tcatctgttt catctgcttc ctccttccaa
    42181 aagaaaaaag tcagcgcaaa ggataccacc atcgcaatgg cgatcccaat caatacatta
    42241 atcacatctg ttaaatcacc atctggtcta atatagttgg gaattgtaaa gatgcctagt
    42301 ccaccttgat tccaagaagt taaattcatt aacccactat acgcgccccc taacgcacca
    42361 ccgacacagg taaaaataaa tattttttta cgtggcaatg taaacccata aatagctggc
    42421 tctgtaatcc ccacaatacc agaaacaatg gctggtattg caattgcttt gtcttttgga
    42481 tttctaagct taaagtagag tgcggtaagt gctgcagttt gtccaaaaga cactactgca
    42541 acaggtgtca taatattttg ccaaccattc acagaaaatt gcatcaatcc cattggtacg
    42601 attgcccaat ggagaccaaa catcaccaga atttgccaga agaagcctac gattgctcca
    42661 taaataactg gcgaaagtgc taaaagtgtt tcaaatccag ccattaaaat atcagttgcg
    42721 aatgtgaata ctggtccaac gagtaaaaaa cctaaaggca ttgtaataag aacggtgaag
    42781 aatggaacga agaaattcgc gatcatctca ggtactaact ttttaattgg tttctgaatt
    42841 tggctagcta acaacacaat aaagataatg ggaatgacag aagaagcgta gttacgcgca
    42901 atccagggaa tgccgaatac tgtttgataa ataggtgctt caaaaatagt gccagaaaat
    42961 atggtcgtta gtggtgcttc tgctgcacca ctcaaagctt ccagcgacaa gctaggattc
    43021 attaatgctg cgccaagcaa catccctacg aattcattta ctttaaattt acgggcagct
    43081 gttaaaccga taataacagg cataaattgg aatacagaat caccgatatt atcaaacata
    43141 acaaatgtgc cagaaccgcg gtcaaacgcg cctaagaaga caaggaatgc tgtcagtcca
    43201 cgaatcatcc cgtcagcagc taggatagca aggaatggtt ggaaacagcc cgatattcca
    43261 tcaataagca tatcaaacgg tctcattttt tctgttgaaa cagcctcatc aaaagtaagg
    43321 tttcctgctt ttataacctc ttcaaacacg actggcacat gattcccgat gacaacttga
    43381 tactgccctc ccgctcgcat taccgttaca ataccgtcca tatttttcaa gatctcatca
    43441 tttgcttttt cttcgttttt taatttgaaa cgaagccttg tgatacaatg tgtcaaacca
    43501 ttgatatttt ccttcccgcc gacattttct acgatttttc tcgctaattc ttggtatttt
    43561 cccatttcta ttccctccaa tttattagtg gaaggtttag ccaactgaat gtcactaccc
    43621 ttatttcata cgttcttgtt aatttatcct ccttttttta tttatttaca acgattctca
    43681 tgcactaaac gcgcagtatg gatcgttaaa tacatttttt cgtccgtgga catctggtag
    43741 ttgaacgtct cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat
    43801 tttttttgaa taacctgaaa caattcctca gaaacttctc ccacttctga tttttctcca
    43861 tctaggacgc gtgtcagaaa aaaacgtaag tgtgtgacga atcgaaaaaa atggactgaa
    43921 tcttcatcaa atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt
    43981 atagtgaaca ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc
    44041 aatgcgataa atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc
    44101 ttcactgttt tttttcccga tggcatactc tttaggaaat agttgtttga tatcatacag
    44161 caatacattt ggtacaaata ttcccatttt gtgacgttcg atagccgtat acaaatgatc
    44221 cgtcaatgca atatacacag aatcattcaa ctggacttgt agagcctctt ttgtatattc
    44281 aatgatttca caagaaattt ctaagtactc aagaggaatc gcttgtgcca attcttggaa
    44341 ctgttgtgaa agcccgtcat ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat
    44401 cgggtcaccc actttctttt tgaatgccaa accttttccc atcacgatca tttcctgatt
    44461 tctttcatcc ctggttatta cgacgttgtt attcagtact ttctcaatca gcatgttttg
    44521 ctcctttcca tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc
    44581 catggaacca tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg
    44641 tatcgttttc tttatattat cgaaagcaaa cgcttttgtc aacgctttct tagtggttat
    44701 tagccgcttt tgactagctg tcgtcgtctt ttttaaatcg ggtgactacg ccactaacgc
    44761 tcacagatga agaatctgcg aagctctgtt ttattttcac tctagcgctt agggcaaaat
    44821 gctactatgc tactgtcaca acctcttttt acacaccact gctttgcagt ttgtacatat
    44881 ctgcatacaa accattttgc gctagaagtg tttcatgatt cccacgctct actatatgac
    44941 ctttgtctaa aactaaaatc aattctgcat cccgaatcgt ggacagccga tgggcaatcg
    45001 ctatcgttgt ccgtccttgt cgcatttttg ccaatccttc ttggatcaat ccttcggttt
    45061 cagtatcgat atttgctgtt gcttcatcca atactagtat tttaggatct gtcacgattg
    45121 ttcgcgcaaa agtgatcagc tgtcgttgtc cactagaata gctggcaccg tgttcgatca
    45181 cttttgagtg atatcctttt ggtaattcat cgatgaactt gtttgcttga acaaactcag
    45241 ctgcatggcg aatctgttcg tctgtgatag ctggattcat catccggata tttcctgcaa
    45301 tatctccata aaacaagaag gcatcttgca agaccaatcc cattttctta cgtaactctt
    45361 ccattggata ttctcgaatg tcatgatcat caatcaagat ttggccttca taaaattcgt
    45421 aaaaccgcat caatacgttg atgatcgagc tttttccgct tccggtgtgc cccactaatg
    45481 caacggtctc acccggatta gcgacaaaag agatattgtt caatacattg tgctctccat
    45541 cataagaaaa gcttacattg cgaaactcga tcttacctga agtaatcgct tgatctgctc
    45601 caggattttg ttgcggtgca tattcttctg tatccatgat tttcaaaata cgactaccgg
    45661 caaccatccc atctgtaaag gtgctcaaaa agtccatcat ttgagacatt ggattaaaaa
    45721 attcctggac ataggtcgta aatgcataga tcagtccaac ttctacggga gaatgcaacg
    45781 catcgatccc aaacaaagtc aacgccaacg caatcgccaa tgcatagaga aaattgatga
    45841 ttggacttaa cagaatcgaa ttcgttttga tcatcgcata gcgcgtgtct aaatactcgt
    45901 tgttcacttg ttcaaattct ttagctaaac gagcttcttg gcgaaactgc tggataatac
    45961 gcatgccgga aatcgattca ttcaatttcg tgttcagctg acttaatttt tcccgcatat
    46021 ttcggtaaat acgagaactg aatttctggt aataccagat gacgattcct aaaatcggca
    46081 agaacaccaa agtccacaag gcaatttctc cattgatttg aaacatggca acgaaagaag
    46141 aaacgactgc aaagatccca gtcaatacca tcaaaaagac ataccaaaat tcaaatagcg
    46201 tttctgtgtc attcgtcact cgagaaacgg tcgatcctgc cggtgtctgg tcaaaataac
    46261 gcatacctag tgtatgcagc ttttcaaata atcttacccg aacatactga taggtcttca
    46321 atgaagccat ggaatacaaa taccattgga aaaaccaaat gatacttttg actatgactc
    46381 caaacaagta cacaccagca aaaaacaaaa taacttgtgt cgtagctgac tgtttagcca
    46441 gatgatcatc cataaaagtt tggatgatcc ttggcaatag gacattgatc acagacaggg
    46501 caaacgcaaa taagatagca acgatgaacg ttcttctaaa aggtttggcg aacttcatca
    46561 gtcgtttgat gatcttcttc tgttctttaa gtggaatcga ttttgtccat tccgattgat
    46621 aattttcatc catcaggcct cgcctccttc tatcttcgct tctaattgct gtttttccca
    46681 cattcgttca taccagccac ctaatgcaag cagctcgtgg tgggttcctc gttcaatgat
    46741 tttcccttga tcgacaacaa tgatttcttt tgcatgcatc acgctgctta aacggtgagc
    46801 tgcaatgatg gttgttttct cttttcttat ttcttttaaa ttatgcaaga tagcttcttc
    46861 tgttttggca tctactgcag aaagtgcatc atctaagatc agcaattcag gatttttgat
    46921 caatgctcga gcaatcgaaa tcctctgttt ctgtccgcca gataaagaga ctccacgttc
    46981 tccaactaag gtatcataac cttttggcaa ttccttgatt tcattgttga taaatgctaa
    47041 agaagccgca tgctctactt cttcttctga aagttttgga ttagaaaaac ggatattatc
    47101 tcgaatggtc atcgaaaaca ggaagtgatc ctgaggcaca taaccgattg atcctaataa
    47161 agcattcaat gaatattctt tgatcgaacg tccgccaaat tggattttcc cttgataatc
    47221 atcgtattca cgcatcagta atttgatgat tgtcgttttt cccgcaccag ttttcccaac
    47281 aatacctaat gtttctcctt cttggatcgc aaatttgatt tgttctaatg ctggtgattc
    47341 ttctttcgga tagttaaaag aggaaatagc tacattcagc tcgccttttg ccggggtctg
    47401 cactgcaccc agttgttcca cgatatgcgt cttttcatgc aaaagttcat tgacgcgatc
    47461 ataagaagca tttcctcttt ccaatacgtt gaacaatcga ccaatcgcaa acatcggcca
    47521 aacaagcata ccgatatagc taataaatga gaccatctgc ccgattgaga tcgtgccttc
    47581 caagacatac tttccaccta aaataattgt tgcaacataa gacagaccga taatcaaggt
    47641 aatgaatgga tcaaataagg agtctaaaaa attgactcgt ttgtttttaa taattgcatc
    47701 ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct tgtccaaatg ttttaatgac
    47761 tttgatccca gtgatacttt cttgtgtctt atcattgata ctagaaaaag cttcttgcga
    47821 atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt gaagtaacgg ctagcaaagg
    47881 cagaggaatc agcgcaatca gtgttaagcg ccaatcaata aacaaaatca tcgcaatgat
    47941 cgttgttccg cccgtaatca ctgaatctgc aaaggtcaag attccagctc ctgctacatt
    48001 ctggattgca ttcaaatcat tcgttgcatg agccatcaaa tctccagtcc gatatttttg
    48061 gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc tgctgccgaa gttctctctc
    48121 caaccgagct gcacttcccc aaatattcgt ccgccagata taacgaaaaa gatattgtaa
    48181 gaaagctgca aggatcaata ataagatcca ccaggaaatg atctttattg ataaattctt
    48241 atcggcgatc tcatcaatga tgatcccaat cacttttgga ggaactaact gtaccaaggc
    48301 cactgcgaat agggagaaaa tcccaattaa ataatgtttc ttttcctgac gaaaaaacca
    48361 acctaatttt ctaaaaatcg acattttact cctcctttgt ctttatcaag agcataggat
    48421 tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc taacaaatta cgaaataaaa
    48481 aacggactga aaaaatcagc ccgcaaaaag tctttgggtc agaacctatt ttttcccttg
    48541 gttcttcatt tgctgcatca tctggcgaat tttcttctca gatggttttt gccccattga
    48601 catcatcatt gatcgcaaca tgtcctcatt aacgggagga tttttttcta aatagtcttt
    48661 catatattta cgcgctagga aaaagccacc tactgcgccg attagcaaag caataatagc
    48721 aataagtacg acaatacctg ttgataccat ataaatctct cctttctatt tccgaattgc
    48781 tctcaatgat ttattttact aaagtttatt gaaaaaagaa aggcgtattc tttagtttca
    48841 tgcaaaaaat agtggttttt ttagagaaga acaaaatttc tttcattaac cagcaagaca
    48901 caccacgaaa aaaaattagg cataccgaat ctttttcttt aattttttct ttctgaggtg
    48961 tagaatgtaa tcgtagataa ataggaaagg tggttttttc ttgaataatc aatccaacaa
    49021 acaatttcat aagcatcgac ttattgaaca tacaaatggt ttatctttag ctgaagtaaa
    49081 tggaacagtg gatgtaccaa agggaaaaag tttttttaaa acacttcttg cttactctgg
    49141 accaggtgca ttagtagcag ttggttatat ggatccaggt aactggtcca cttcgatcac
    49201 tggtggacaa aatttccaat atttattgat gtcagttatt ttgatgtcta gtctaattgc
    49261 tatgctttta cagtatatgg cagcaaaatt aggaatcgtg acccaaatgg atttggccca
    49321 agcaatccga gcacggacaa gtaagacatt gggtgtcatt ttatggatct tgactgaatt
    49381 agccattatg gcaacagaca tcgcagaagt cattggtgca gcaatcgccc tctacttact
    49441 ttttgatatt ccattggtga ttgccgtctt tattactgta ttagatgttt tcgttctctt
    49501 actactgaca aaagtcgggt ttcgtaagat cgaagcactg gttgtctgct taattttcgt
    49561 cattttattc gtttttgtct atcaagttgg cttgtctaac ccagattggc acagtttatt
    49621 tgctggcttt atccctaaca gtcatacatt ctcagactca ccggtcatcg gcggcgaaac
    49681 tccgctgact ggagctttag ggattattgg tgcaacagtc atgccgcata acttatatct
    49741 tcattcagca gtttcacaaa ctcggaagat cgatcaccaa aacgaagaag atgtagcaca
    49801 agctgtacgt ttttctactt gggattctaa tatccagttg tcaatggctt ttctagtcaa
    49861 tgcactgcta ttgatcatgg gtgtcgctgt cttcaaaacc ggttctgtcc aagatccttc
    49921 cttttttggt ctgtatgaag ctttatctga tccatcagct atgagcaatg gtattttgat
    49981 tgctgtagca aaatctggta tcttgtccac tttatttgct gtagcacttc ttgcttctgg
    50041 ccagaactct actattactg gtacgttgac cggtcaagtc atcatggaag gattcatcca
    50101 tatgcgtatc ccgatttggc taagaagact ggtcacacga ttgctttccg tgattcctgt
    50161 attgatttgt gttttgttta caagtacaaa aggaacgatc gaagaacatg ccgctttgaa
    50221 taacttaatg aatgaatccc aggtgttctt ggcttttgcg ttgccatttt cgatgattcc
    50281 tttactgatg atgaccaaca gccaagcgga gatgggtagc cgatttaaaa attcattcat
    50341 cgtaaaactt cttggctggt tctctgtgat tagtctaact tacttgaact tacgaggatt
    50401 accaggacag atcgaaagtt tcttcggcga gcaagccact tccgctcaac tagcattggc
    50461 tgatcacatc gcttatgtaa tcattgttct tgtccttctc ctgttggcat ggacgattgg
    50521 tgatttatat aaaggaaaca aaaaatatga acagcttttg caggaaacgg tagaagaaat
    50581 cgaataaaaa ctgatttttc cacacaaaga cccttgatag ttaggttaaa aatctagcta
    50641 ccaagggtct ttttatttag aagataaagc ttttttcaga tttaatttac tgaaaactag
    50701 catgcttgtt actacgatga attgcgttaa tggcacgcct gcaccagtgt agacacttaa
    50761 aaaatagtcc tttttttctg gcgcataaga acaaaaccat gagacaccga aaagaaaaac
    50821 caattgtgtc agtattaatc cggtgaccac aatcaaagag gaactataat aggcacgatc
    50881 agcttttgca aatacggttc ctagtatcat aagtaatatc cctgctattg aagcaatcag
    50941 cacgggaata gccagaactt ggtaaatttt tatgatgaca ttcgcgactt ttgctgattt
    51001 tgttaattgg aaattaggtg ttctcggttc attactatcg atccaattct ccattagcgg
    51061 aaggtttaat aattcttgag cctgtttgac ttgttcttgc gttccgaaac tataatttcc
    51121 tccttgatgg aaatctttat atcgccatgc ataagcgtat cctgattgaa ggaaatcgaa
    51181 tacgattggc agatcactca tcgtctttcc atctcctgat cccatcagat agaactcttt
    51241 tttctttttg atggttccct tgtgataggc ttcttctaat tcttggttga cttttttcca
    51301 aaaatcattg gtttcttttg cattgttttg gtaataacca gattcgctta ccacatctcg
    51361 caatgcccaa aagatgatat ctccgggtaa ttcttttact tttccacctt gagtccacgc
    51421 gtgagtcgta tataattcgt caatacgggt agaaacagta tggaatgtag gagatgcggc
    51481 taatgcttta tttagtgcag catgtgagac ccatatctca ctgttttctt tattcaagtt
    51541 cgtttcatca tctattcgga tcagttgttt catcaaacgt ccaaattctc cactggaacg
    51601 gtcattgatg gttgcaatac cgtatgttgt ctcattttta tattcgatcc actgatagac
    51661 gatggaaaag ccaactatag gaagtaaaaa caatgaaagt ttcagccatt cccgtttcga
    51721 gagaagaaag agtcgttttt ttaacttctt tacttctttg aaagaaaagt cttgccctag
    51781 caagatctgg acgaaactga tacccaaagc tactacagta aaaggaagaa gccagataga
    51841 atcttcgcga atgaaccaaa agaaaggaaa agcgacagaa agccctaaaa accatggcag
    51901 caattttctg ctagaaccgt atttgcggta ataaatagct aataaacaag cgataatgat
    51961 ctcaacagcc ggaatcacga gggcattacg atatgttcga gtggaatatt catgtgtaaa
    52021 actaatcgga gaaaacaaaa aataaaaata gatacttaaa agaagatact tgttcttcac
    52081 aatcggttta aacgcgagtg caaccaatat actggcaaaa atactgaaca tgccgaacaa
    52141 aactgcataa gaaagatgca gtttatgtgc aagtactaaa aataagctgt aggagattcc
    52201 tttggaaagt gttgtagtcg tatactcccc tagccattta ccatccaaaa tattttgagc
    52261 atagtgaatc agcagctgat catcataggt cgccgacata tctgttccat agattgcttt
    52321 actatataaa tacaaccgaa taattgtaaa taatacggct atgccaagaa taaagtatgt
    52381 atgtttcttt tttagttggt cgatcaaggg gtttaaaatg ttcagcatct tttcgtcctt
    52441 tcaatcacgt tttgctactt gtttgtgaaa cttttcgttt tttacttttc tatcagtata
    52501 ccctgtttgt ctgaatttaa aaagaggggg caaaaggagc atggcgaaaa cgaatgcaaa
    52561 aagcactcct ttgtttctta aacaaaaaag gacaaggcag actgcccaat ccttttttgt
    52621 tgctgtcagg tagaaaactt aaagtgtgac ttcttctgct aataactcag ggaacacttc
    52681 gggatcgaat gcttgcgctt tgaacatgga aatttctttt ccatatggtg cgtaagcttt
    52741 ctttttatct ttaccgacat atggggtttc taatattttc gaaatgttgt caaacattgg
    52801 atcatggacg attttgttca atgtgtcaaa tccaattgtt ccaaaaccga tattggcatg
    52861 ccgatcttta tgagaacctt gtggattttt agaatcattg atatgcagta ctttaagccg
    52921 atctaatccg atgaccttat caaactcttc cattacacct gaaaaatctt cttttatatt
    52981 atatccggca tcattcgtat gacatgtatc aaaagtaacg gataattttt cattcaattt
    53041 tactccatcg atgatttgcg ctaattcctc aaatgttcga ccgatttccg tacctttccc
    53101 tgccatcgtc tccaaagcga tctgtgccgt ctgttctttt gtcaaaactt cattcaaccc
    53161 ctcaataatt ctagcaatcc cagcttctgc ccctgctcct acatgagcgc ctgggtgaag
    53221 ggtgatttga gtagcaccta aagcttccgc acggacaatc tcttctctca gaaattgtac
    53281 agcaaatgga aacatttctg gtttgatcgt atttcccaaa ttaatgatat aaggcgcatg
    53341 aacgacgata tttgttagtc cgttttctgc cataaatgct tttccggcat caatattcat
    53401 ctcttcgata gcttttcttc tagtattttg aggagcgcct gtatagatca taaaggtcga
    53461 agatccgtaa ctggctgctt cttctgccgc gcctaacagc attttctttc cgctcatgct
    53521 gacatgtgag cctaataaca tggatggatt cctccttgaa aggcaattgt ttcgaacaat
    53581 tcctgtatat tgataagatg atgtacaaag aaaatggttt gtttttcttt gccctgttct
    53641 ccatagtaac tgaaactttc cctaaagaaa agcttaagaa ctctttcata aaaaagatca
    53701 tttcctcacg ttttatctca ttcgaaaaag aaattcctgt caaaaacagc cttgtctgtt
    53761 tttttgtcct attatttgct ataattagcg aaataaaacc attagttgag gtgacccttt
    53821 tgccacgcaa aaatactcga aaaaaacaga atagaagaaa aaaagaaaaa tggtttgttc
    53881 ctaagattat ttttagagtg tttcaatcat tgactgtttt tattactgtt cttcttttgt
    53941 tgttcgcagc tctaggtatc ggaataggtg ctggctactt tgcctatctt gtcgaagaca
    54001 cacaattgcc gactaaaaaa gaactgcaga cagaactagg aaacattacg gaaacgtcaa
    54061 agattgttta tgcagataat acagagatat caaaaatcca gacagatctg atgcggacga
    54121 ctatttcatc tgataagatt tcgcctttat tgaaaaccgc gattatcagt acagaagatg
    54181 aatacttcga taaacatcaa ggttatgtcc ctaaagctgt tttacgagca ctagtttcag
    54241 aagcgactgg catcggctcg tctggaggat ccactttgac acagcaatta gtcaaacaac
    54301 agatcctaac cgatgaaaca acattcaaac gtaaagcaaa cgagatcgtt ctttctgcac
    54361 aagtagaaaa acacttttca aaaaatgaga tcattactac gtatttgaat gtctcaccat
    54421 ttggtcggaa taataagggg caaaatattg ctggagtcca ggaagctgct agaggaatct
    54481 ttggtgtaga tgcaaaggat gtgactctgc cacaagctgc ttatatcgct ggtttgcctc
    54541 aaagccctat cacgtatagt ccatacacga atacaggcgc gctaaaagaa gatctttctg
    54601 ccggtcttgc cagaaaagat tttgttttat tcagtatgta ccgtgaagga caaattacga
    54661 aagaacaata cgaagaagcg aaagcctatg atcttacaaa agactttctc ccacaacaaa
    54721 tcgcggaaca gactgacaga gaattcttgt attataccgt gatgaacgaa gcaacaagga
    54781 ttattgcaca acaattggca gaaaaagata atgctgatat gtcagattca tcggtcagtg
    54841 aagcttatta tcaaaaagca cagcaaacga tccaaaacaa aggatatacg atccattcga
    54901 cgatcgataa agatatctat gctgcattac aaaccggcgt agaaaattat ggctacatgt
    54961 tagatgatgg tgctggtagc caagtggaga ctggaaatgt attgatggat aacagaaccg
    55021 gacgtatcta tggcttcgtg ggtggtcgga attacagcca gaatcaaaat aaccacgcct
    55081 ttgatacaga acgtcaagct ggttcatcca tcaaaccggt tcttgtttac ggtcctgcaa
    55141 tcgatatggg acttgtcgga tcagaatccc gtgtatcaga ttatgcaacg acttggcaag
    55201 aaggaaaaaa tgctggtgag aaaatcgtca atgcgaccaa tgaagggtcg aacaccttcc
    55261 aaaccgtccg tgagtcactg gaatggtcca acaatatccc agcttatcac ttatatcaag
    55321 atgtgttgaa ttccggtgga tcgaaacaat acgcttacga gcactacttg gcgaagatga
    55381 actacccagc aaatgataat tggggcgttg aatcggctcc tttagggaca gtagatgtaa
    55441 cgacgcttca acagacaaat ggattccagg ccttagcaaa tggtggagtc tacgaagaag
    55501 gctatatcat tgattccatc acagataatg caggtaatgt gatttataaa catgaaagta
    55561 attctgtccg tatttattcc gaagcaacgg catcgatcat gaatgacatg atgcgtagtg
    55621 tgatcaatga aaaaatcact acaccattca aagatgtcat ctcaagctta aacggaaatc
    55681 taggaaatgc agattgggtc ggaaaaactg gttctaccaa tgaataccga gattcatggc
    55741 tgatcgtctc aactccatct atcactatca gtagttgggc aggacacgat gacaatactg
    55801 gaatggacag ccaagcaaga atacgttcgg ctaattatct tgccaatcta atcaatcaag
    55861 cttaccaagc aaaccctgag attttcggca ctgatgagaa atttgactta tcatccgatg
    55921 tgatgaagaa aaaagtatct gggttcacag gacaaacagc tggaaaagtt actgtcaata
    55981 aaaaatccat ccaaacgcca ggaaaaacag tggagtcact ttgggcaaaa aatggtccga
    56041 agaaaagcgc ctttaaattc ggcgtaggcg gtactgatga aaactataca gattactgga
    56101 acaccgcttc agcttatgct agagcgaatc cagagaccaa agaatcagac gataaagatg
    56161 attgatctaa tagattaatc agaattaaag ggagctgcga caaaaatctt gtcacagctc
    56221 cctttttccg aataaatggt gttcaaaagc ttacatctac gatattaaga caaatttatg
    56281 taaaaatgtg aaaaac
//
LOCUS       DACEKG010000007        54448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEKG010000007 DACEKG010000000
VERSION     DACEKG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.007"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..590
                     /locus_tag="IW664_000353"
     CDS             <1..590
                     /locus_tag="IW664_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847745.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(830..913)
                     /locus_tag="IW664_000354"
     tRNA            complement(830..913)
                     /locus_tag="IW664_000354"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Leu,seq:gag)
     gene            1296..2513
                     /locus_tag="IW664_000355"
     CDS             1296..2513
                     /locus_tag="IW664_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0847746.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2697..3533
                     /locus_tag="IW664_000356"
     CDS             2697..3533
                     /locus_tag="IW664_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0847747.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3609..4757
                     /gene="nagA"
                     /locus_tag="IW664_000357"
     CDS             3609..4757
                     /gene="nagA"
                     /locus_tag="IW664_000357"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0847748.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4978..6036
                     /locus_tag="IW664_000358"
     CDS             4978..6036
                     /locus_tag="IW664_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0847749.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6055..7074
                     /locus_tag="IW664_000359"
     CDS             6055..7074
                     /locus_tag="IW664_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0847750.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7176..7892)
                     /locus_tag="IW664_000360"
     CDS             complement(7176..7892)
                     /locus_tag="IW664_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0847751.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7861..8646)
                     /locus_tag="IW664_000361"
     CDS             complement(7861..8646)
                     /locus_tag="IW664_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0847752.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8637..9386)
                     /locus_tag="IW664_000362"
     CDS             complement(8637..9386)
                     /locus_tag="IW664_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0847753.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9684..10208
                     /locus_tag="IW664_000363"
     CDS             9684..10208
                     /locus_tag="IW664_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847754.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10224..11723
                     /locus_tag="IW664_000364"
     CDS             10224..11723
                     /locus_tag="IW664_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0847755.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11811..12413)
                     /locus_tag="IW664_000365"
     CDS             complement(11811..12413)
                     /locus_tag="IW664_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0847756.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12517..13473
                     /locus_tag="IW664_000366"
     CDS             12517..13473
                     /locus_tag="IW664_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0847757.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13778..14734)
                     /locus_tag="IW664_000367"
     CDS             complement(13778..14734)
                     /locus_tag="IW664_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0847758.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14908..15468
                     /locus_tag="IW664_000368"
     CDS             14908..15468
                     /locus_tag="IW664_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847759.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15461..16285
                     /locus_tag="IW664_000369"
     CDS             15461..16285
                     /locus_tag="IW664_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0847760.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16295..16912
                     /locus_tag="IW664_000370"
     CDS             16295..16912
                     /locus_tag="IW664_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0847761.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16930..17613
                     /locus_tag="IW664_000371"
     CDS             16930..17613
                     /locus_tag="IW664_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0847762.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18296..19579
                     /locus_tag="IW664_000372"
     CDS             18296..19579
                     /locus_tag="IW664_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0847763.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19595..20482
                     /locus_tag="IW664_000373"
     CDS             19595..20482
                     /locus_tag="IW664_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0847764.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20499..21335
                     /locus_tag="IW664_000374"
     CDS             20499..21335
                     /locus_tag="IW664_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0847765.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21338..21961
                     /locus_tag="IW664_000375"
     CDS             21338..21961
                     /locus_tag="IW664_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847766.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21958..23250
                     /locus_tag="IW664_000376"
     CDS             21958..23250
                     /locus_tag="IW664_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0847767.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23609..23706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23822..24403
                     /locus_tag="IW664_000377"
     CDS             23822..24403
                     /locus_tag="IW664_000377"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0847768.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24406..25713
                     /locus_tag="IW664_000378"
     CDS             24406..25713
                     /locus_tag="IW664_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0847769.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26129..26617
                     /gene="purE"
                     /locus_tag="IW664_000379"
     CDS             26129..26617
                     /gene="purE"
                     /locus_tag="IW664_000379"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0847770.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26601..27734
                     /gene="purK"
                     /locus_tag="IW664_000380"
     CDS             26601..27734
                     /gene="purK"
                     /locus_tag="IW664_000380"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0847771.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27747..29042
                     /gene="purB"
                     /locus_tag="IW664_000381"
     CDS             27747..29042
                     /gene="purB"
                     /locus_tag="IW664_000381"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0847772.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29222..29599)
                     /locus_tag="IW664_000382"
     CDS             complement(29222..29599)
                     /locus_tag="IW664_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847773.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29855..30583
                     /locus_tag="IW664_000383"
     CDS             29855..30583
                     /locus_tag="IW664_000383"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0847774.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30583..30837
                     /gene="purS"
                     /locus_tag="IW664_000384"
     CDS             30583..30837
                     /gene="purS"
                     /locus_tag="IW664_000384"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0847775.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30839..31510
                     /gene="purQ"
                     /locus_tag="IW664_000385"
     CDS             30839..31510
                     /gene="purQ"
                     /locus_tag="IW664_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0847776.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31511..33733
                     /gene="purL"
                     /locus_tag="IW664_000386"
     CDS             31511..33733
                     /gene="purL"
                     /locus_tag="IW664_000386"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0847777.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33718..35157
                     /locus_tag="IW664_000387"
     CDS             33718..35157
                     /locus_tag="IW664_000387"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0847778.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35189..36232
                     /locus_tag="IW664_000388"
     CDS             35189..36232
                     /locus_tag="IW664_000388"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0847779.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36229..36807
                     /gene="purN"
                     /locus_tag="IW664_000389"
     CDS             36229..36807
                     /gene="purN"
                     /locus_tag="IW664_000389"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0847780.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36804..38339
                     /gene="purH"
                     /locus_tag="IW664_000390"
     CDS             36804..38339
                     /gene="purH"
                     /locus_tag="IW664_000390"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0847781.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38355..39629
                     /gene="purD"
                     /locus_tag="IW664_000391"
     CDS             38355..39629
                     /gene="purD"
                     /locus_tag="IW664_000391"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0847782.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39751..40665
                     /locus_tag="IW664_000392"
     CDS             39751..40665
                     /locus_tag="IW664_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0847783.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40798..42363)
                     /locus_tag="IW664_000393"
     CDS             complement(40798..42363)
                     /locus_tag="IW664_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0847784.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42356..43807)
                     /locus_tag="IW664_000394"
     CDS             complement(42356..43807)
                     /locus_tag="IW664_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0847785.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43821..44837)
                     /locus_tag="IW664_000395"
     CDS             complement(43821..44837)
                     /locus_tag="IW664_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0847786.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45057..46145
                     /locus_tag="IW664_000396"
     CDS             45057..46145
                     /locus_tag="IW664_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0847787.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46199..47725
                     /locus_tag="IW664_000397"
     CDS             46199..47725
                     /locus_tag="IW664_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847788.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47727..48932
                     /locus_tag="IW664_000398"
     CDS             47727..48932
                     /locus_tag="IW664_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0847789.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49189..50166
                     /gene="guaC"
                     /locus_tag="IW664_000399"
     CDS             49189..50166
                     /gene="guaC"
                     /locus_tag="IW664_000399"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0847790.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50430..51854
                     /locus_tag="IW664_000400"
     CDS             50430..51854
                     /locus_tag="IW664_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0847791.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52315..53424
                     /gene="pdhA"
                     /locus_tag="IW664_000401"
     CDS             52315..53424
                     /gene="pdhA"
                     /locus_tag="IW664_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0847792.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53427..54404
                     /locus_tag="IW664_000402"
     CDS             53427..54404
                     /locus_tag="IW664_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0847793.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17403 a         9316 c        11921 g        15808 t
ORIGIN      
        1 tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat
       61 ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc
      121 tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa
      181 gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac
      241 tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca ttattactat
      301 gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta
      361 ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg
      421 cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg
      481 cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat
      541 taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa
      601 atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca
      661 cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta
      721 catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa
      781 cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg
      841 ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt
      901 ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag
      961 ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg
     1021 aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct
     1081 aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga
     1141 caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt
     1201 ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa
     1261 gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca
     1321 aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc
     1381 aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg
     1441 aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact
     1501 cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac
     1561 attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca
     1621 cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact
     1681 atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg
     1741 atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga
     1801 caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat
     1861 tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa
     1921 tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg
     1981 atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag
     2041 taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga
     2101 atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg
     2161 cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac
     2221 aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa
     2281 aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg
     2341 aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat
     2401 ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga
     2461 aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa
     2521 aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga
     2581 gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac
     2641 ttcctaaagt aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg
     2701 aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga
     2761 cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata
     2821 atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga
     2881 ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac
     2941 tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt
     3001 ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg
     3061 gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag
     3121 cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga
     3181 ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt
     3241 tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt
     3301 cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt
     3361 tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca
     3421 atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag
     3481 cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt
     3541 agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag
     3601 agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac
     3661 atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg
     3721 agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata
     3781 cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag
     3841 tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt
     3901 caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac
     3961 caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag
     4021 gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag
     4081 aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag
     4141 agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta
     4201 cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca
     4261 atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag
     4321 aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa
     4381 tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg
     4441 gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa
     4501 cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta
     4561 aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga
     4621 ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg
     4681 cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg
     4741 aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt
     4801 tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt
     4861 aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat
     4921 tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg
     4981 aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact
     5041 tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa
     5101 tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt
     5161 cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt
     5221 tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat
     5281 aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc
     5341 ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc
     5401 aatattggct accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag
     5461 atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc
     5521 ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa
     5581 ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag
     5641 ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca
     5701 ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta
     5761 gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg
     5821 aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca
     5881 gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc
     5941 aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta
     6001 ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga
     6061 atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac
     6121 cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag
     6181 gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa
     6241 atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat
     6301 ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat
     6361 gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg
     6421 acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc
     6481 agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg
     6541 tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa
     6601 aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt
     6661 catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa
     6721 atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct
     6781 attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat
     6841 gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc
     6901 cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca
     6961 gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa
     7021 actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac
     7081 atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta
     7141 tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc
     7201 cgcccattct ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt
     7261 ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt
     7321 gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata
     7381 aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat
     7441 ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct
     7501 ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa
     7561 cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt
     7621 atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt
     7681 ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc
     7741 agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg
     7801 attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga
     7861 tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag
     7921 atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt
     7981 gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc
     8041 tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt
     8101 cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct
     8161 ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa
     8221 aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag
     8281 catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc
     8341 aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg
     8401 ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg
     8461 aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc
     8521 cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc
     8581 taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa
     8641 atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc
     8701 atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag
     8761 aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag
     8821 attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct
     8881 tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg
     8941 atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact
     9001 tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca
     9061 taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa
     9121 aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg
     9181 taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct
     9241 aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca
     9301 gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt
     9361 ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa
     9421 acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt
     9481 aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat
     9541 tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa
     9601 aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg
     9661 tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt
     9721 atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg
     9781 attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat
     9841 ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg
     9901 gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac
     9961 atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga
    10021 tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca
    10081 ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag
    10141 tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa
    10201 aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg
    10261 cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac
    10321 cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg
    10381 aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc
    10441 aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt
    10501 atacggaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat
    10561 ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg
    10621 aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg
    10681 tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg
    10741 tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca
    10801 aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag
    10861 atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag
    10921 aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag
    10981 aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag
    11041 cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta
    11101 aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa
    11161 agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag
    11221 aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg
    11281 aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag
    11341 cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca
    11401 aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc
    11461 gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca
    11521 gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt
    11581 ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt
    11641 tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac
    11701 aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa
    11761 gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg
    11821 ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat
    11881 cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca
    11941 ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt
    12001 acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac
    12061 aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc
    12121 acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag
    12181 cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta
    12241 gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat
    12301 gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca
    12361 gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt
    12421 ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt
    12481 attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc
    12541 aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat
    12601 tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg
    12661 cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt
    12721 ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg
    12781 cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat
    12841 tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg
    12901 attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg
    12961 aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa
    13021 tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc
    13081 attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg
    13141 ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg
    13201 ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta
    13261 ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt
    13321 attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc
    13381 gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag
    13441 gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg
    13501 tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc
    13561 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    13621 ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc
    13681 ggtaataagt ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
    13741 acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt
    13801 gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt
    13861 caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc
    13921 cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg
    13981 ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt
    14041 ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga
    14101 cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt
    14161 gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc
    14221 ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga
    14281 aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact
    14341 gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt
    14401 ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata
    14461 gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta
    14521 cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag
    14581 cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa
    14641 ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac
    14701 caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat
    14761 tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact
    14821 tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag
    14881 tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt
    14941 gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt
    15001 ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag
    15061 aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa
    15121 acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca
    15181 cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa
    15241 aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg
    15301 ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga
    15361 gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag
    15421 gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt
    15481 tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc
    15541 gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa
    15601 agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac
    15661 gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga
    15721 tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata
    15781 cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc
    15841 tggatttcca ttagtgatgg tctcagctta ccggtctgta tcatcccaac aacaagtttt
    15901 cgaacaaaat gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg aggcgacaaa
    15961 gattactaaa gaaacgatta cggagccagg atacagcgaa caccacacag gtctggcagt
    16021 cgatgtcgtc gatcaaaact ggtacaacag ttatccttct actgtattgg atgcaagtta
    16081 cggagatcag cctggagcta aatggatcgc agataatgct gcgcagtatg gatttatcgt
    16141 tcgttatcca aaaggacacg aagatataac taagatcaca tatgaacctt ggcacttacg
    16201 ttatgtcggt aaagaaaacg ctgagtatat caccaaacac caattgacgc tagaagaata
    16261 tctgaatgaa ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt ggaaaaagca
    16321 aaagcgcaag aatatccgtt ggccagcagt ggttgccggt gtagcagtga tactcttagc
    16381 ctttgttttt tccttgaaga atctgtcttc tccttttact gataatcagc aagaaaatga
    16441 agcgcaatcc catcagcaat ttattgaacg gcttgttccg catgcccaag aactacaaga
    16501 tggttatgga gtgcttccta gtatcatttt aggacaagct atattagaat caaattgggg
    16561 aaaaagtacc ttggctagca aatacaataa tcttttcggg atcaaggctt atggggatca
    16621 gaagaaagta agcctggaaa caaaggaatt tgttaacgaa gaatggataa ctatccaagg
    16681 agacttcaaa gtgtatgatt catgggaaca gtccatggat gaccatacac aactgttcgt
    16741 ccaaggagta gattggaatc ctgcgctgta tgaaaaagtg attacggcca ccaactatca
    16801 ggaagccgca caagcattgc aagatgcagg ttatgcgaca gaccccggtt atgcgcaaaa
    16861 aatcatccag gtcatcgaga cgtatcaatt gaatcagtat gaccggagat agcaaaggag
    16921 aagtggctga tggcagaaca atttacgcca gaaatcatga cggaattgag aaaagatatc
    16981 gtaagagtac cggaaatcat tttagaagca agcgggatca agatatttgg aaaattgatc
    17041 cgttcgatca tctttactac ggatattgcg atcatacgta atacaaatgc gaatgcagta
    17101 attgctgtgt atccttttac acctcatcca gcgattacta aaagcattat tgaggcagct
    17161 gacattccag ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg ttcttcctac
    17221 atgagcatgt tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg tcctacgcct
    17281 ttagagacag taaaagaagt ttgtcgagta gttgatattc cagttatcag tacggtaacg
    17341 tcgaaatata cagaaataga tgaaaagtta gcagctggta tttcgattat caatatcagt
    17401 gcaggtaaag aaactgcaga agcagtacgc cattttcgta aaaaatatcc tgaacttcct
    17461 atcattgcga caggtggtcc aacggaagaa agtattgctg agaccattca agcaggagca
    17521 aatgccatca catatacgcc accaagtagc ggagagcttt ttagtcgtaa aatggaaaaa
    17581 tatcgggaaa ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag acgaggaaga
    17641 gcagatagtg gctcttcctc gtcttttgta ctccagtact atttgttcag aattcaaaaa
    17701 aaagagaaat ttttttgttt ttttacctgt aatgtacata agaagagtta aaaccttttg
    17761 ttattattag cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt ctatagaatg
    17821 aaaaacgaat aaaaactaaa aaaggcaccc gacaatttag tagcagctaa attgtcgggg
    17881 cttgaaaagt cagcgggaac tgaccaatca agttgccata gtcaatgctt gtgcatcgac
    17941 gtgtatattg tacctaattt catacagaat tactagttat ttttctgttt tttagggaag
    18001 agcgcacacg aagaagcatg cttatttagc tatgtcaagg atattgggca gttgtctgat
    18061 atcctttttt ctttgcgtaa ttatcctccc atcattgaag caagaagtgt ttttagcgat
    18121 gaaagaagaa tcagcactat ctaatttttc ttgaaagtat tgagttgttc atcctaggca
    18181 gaacgataag gcaagtcttg gtttttattc cccttgaaaa agatcgttta ataaatatgt
    18241 tctgtataaa aatacagtat attgaaaaat aacaatggaa gtagaggtgc agagaatgaa
    18301 gaaaagaatg agaatcatct tagctttaag tgttatgtta ttcttcttga cggcttgtgg
    18361 gcaagaagat gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc aagtccgaca
    18421 cgatgcaaca gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg tgacgatcga
    18481 ttatgaattt tatgattatg aaggatatta cacaaaatta ggtacactag cagcatcaga
    18541 tgatatctgg gacattttcc aactgaacaa tcaatttcct caatacatca atcagattga
    18601 accattagat gaatatgtgg agcaagggat aattgatatt tctaatgcag atgaatccta
    18661 tttagcaacg acgacttacg acgggcaatt agttggactg tctaacgggg tgaattcgtt
    18721 tgcgattgct tacaataaaa atatatttga acaattaaat atccctgaac cagcttcaaa
    18781 ttggacatgg gatgaatttg aacaaatcag ctatcaaatc actgaagaat taggaatcta
    18841 tgctatttca agattcgaag attttatagc tggctgtatt attcaaatac cacaagttga
    18901 gaaaggattg aatttttata atcgggaaga tctatctaac agcttgggat ttgaaaatcc
    18961 agactatcta acagattttt tccagctgcg taaaaatctg gtagacaaag gggcccatcc
    19021 agaaccaggt ggtggagctg caagtacgac ggatctgtct tttcaggcgg ttagggatag
    19081 agaagcggca atggtgtttc tatcaagtaa ccagttgaca gaaatattgt caggggctcc
    19141 tgaagatatg gagattcagt tgattaatcc acctcgcaga aaagcagatg gagaatctgg
    19201 tatcccttta cgttcttcgc aaatgctgag tatggcaaaa agctcagccc ataaagaaga
    19261 agctgctaag tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa ttttaaaagg
    19321 agaacgtggt gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg cagatgatgc
    19381 aactttgcaa gcgacatttg attatttaga aatgatttcc gatatggaca acggcgaagt
    19441 gaatgtcttt gatagtgctt tgaatccgga aattcaagac caatacaatc ttttagttga
    19501 aaaagtcatt tatgaagaag taacagcaga agaagcagca gagagtcttt atgagtttgc
    19561 gtcaaacatt ctaggatagc agcaggagga aggcatgaaa aaagaaaaac taaaacggtt
    19621 cttggatcaa gacaatgtgg tcggttacgt atttgcttct ccattcatcc taggatttct
    19681 attgataaca gtgataccga tgatcttgtc actttattat tcgttcaccg attatcgttt
    19741 gggaaatcca gtctcatgga tcggcttgga taattatata aagatgctga gtgatgcccg
    19801 ttttttgaac tccatccgag tcacttttct ctatgtattt gcatctgtac ctgtgaaact
    19861 gatctttggt ctattgattg cttatctttt aacgaggaat atcaaaggaa cgactttctt
    19921 tcgttctttg tattatgtgc ctagtttgat cggtggcagt atttctatcg ctctagtttg
    19981 gcgggagttg ttttctaaaa acgggttagt gaatattttc ctggaacgat taggcgcacc
    20041 tgcgctttcc tggtttagcg atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc
    20101 ttggcaattc ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga tcccaggctc
    20161 gtattatgaa gcggcagaga tcgatggagc gaacaaatgg cagacgtttt acaagatcac
    20221 gttacctctt ctttcgccga tcattttgta taacttggtt atgcagacaa tcgcggcgtt
    20281 tatgactttt acgcaggcat ttatcattac ccaaggtggt ccgaatgatg ccacgaactt
    20341 ttatgctctt tatgtgtata accaagcttt ttcttttaat tctatggggt atgctagtgc
    20401 tatttcgtgg atcatgctgt tgattatggg agctgtcacc tttctgattt tcaaatcatc
    20461 aaaaaaatgg gtcgttgtgt gaatgagagg aggaagaaat gaataataca gtaacgaaat
    20521 ggtttcaata cggttttatt ttgctattag gtctagtcat gatttatccg gtcttatgga
    20581 tgatcgcagg ttcttttaaa acggatcaag agattatgag tggttcgctg aatttgattc
    20641 cagagacttt tcaatgggaa aattacgcaa aaggatgggc ggggtttgct ggaatttcat
    20701 tttttacttt ctttaggaat tcgtttgtca tctctgcagt ttcgacgata gggacagttc
    20761 tgagctcgac ttgtattgct tacgcattat cgagaatcga tttcaaagga aaaagattct
    20821 ggttcgtcgt catgttagta acgatgatga ttcctgcaca agtgattttg attccgcagt
    20881 ttattattta caatcggctg aatttggttg ggacgtatgt gccgctgatt ttaccacatt
    20941 tttttggaca agcatttttc atttaccaga tcatgcaatt catggtcagc atccccaaag
    21001 agttagatga atcagcgatt attgacggat gcagcaagta tagtgtcttt accaaaatta
    21061 tttttccttt gctgaaacct tcgatcatca cgacgatcat catccagttt tattggaaat
    21121 gggacgactt catgggacca ttgatttatt taaacaagcc gagatcttac acggtatcga
    21181 ttgccatcaa attatttgca gacgctggat ctagtacgac ttacgcgagt atgtttgcga
    21241 tgtctacgtt gtcgctgcta ccggtttttc tgattttctt attcttcaat aaatatcttg
    21301 tccaggggat cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa tagaagagat
    21361 gtttctttac aagctcaact actatgtctg tcggattttt ttaaccagtc ttatcagtaa
    21421 tgctcttttc gctttagttc taggatgttt gttcacttta ggattagtgc tgtctgctcg
    21481 tgttgtctat ctgatttatt ttttgatgat gtatccttct tattatctat ctgtccatta
    21541 cttggcagaa aaaaaggtat gttggaggaa aggaaaaatc aaacggatgt gtttcatcat
    21601 tacgttgttt cagctgtttg tcattatttt tgcagttgat tggtatttat tagtgggaaa
    21661 gattggagct tggtatctga ttccttttct attgtgtctg ttgctttttg tatcccatag
    21721 tttttttagt acgctaattg atatctgcaa agaagataaa gcagatagtc ttgtttattt
    21781 gatcaaaagg aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg
    21841 ccttttgtat ggactgttct ggtgtataca gcatgatttt ttactcactg cattactgta
    21901 tccagggctg ccatttgtca cagtattttg cttaaaagaa aaggagaatg actttaaatg
    21961 aagaaaaaat atgtacaagt cggacttggt gggagagcgc gtttttttta tgaggcaatt
    22021 gcctctacgt atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg
    22081 atggattatg caaatcggat acttgtgaac aagtatggac agaaaaaagt aaagacatgt
    22141 atgccatcac aatttgagca actactggat gacgtacggc cggctgaagt cattattacg
    22201 tcagtagatc gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt
    22261 atttgcgaaa aacctctgac tatcgatgag gaaaaagctc aagaaataat aacggtccaa
    22321 aaacagacgg gaagaagcct gcgagtcact tttaattatc gttatgcacc gtatcacacg
    22381 aagataaagg aactgataga aagtgatgtg attggtgatg tgttttctat ccacttcgaa
    22441 tggctgttaa atacgcaaca cggggcagat tatttcagaa gatggcatcg aaataagcac
    22501 aatagcggcg ggcttcttgt tcataaatct acccatcatt ttgatctagt gaatttttgg
    22561 ttgaacagcc gtccccaaaa ggtatttgca ttcggtggat taaactttta tgggatgcat
    22621 aatgcggaga aacgcggagt agaaatattt tacagtcgat gctatggaag cgaactagct
    22681 aaagctgatc catttgctat agacctgtca gccaatgagg aattgcgaga aatgtattta
    22741 gaagcagaaa aagacagtgg atatattcgt gaccgcagtg tattttccga tgatatctct
    22801 attgaagata cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg
    22861 ctaaacgcgt atatgccgtg ggaaggattc aatgtttctt tcaatggcag caaaggacgg
    22921 atcgaaatga aagtgatcga aaagtcttac atcaatgcag gtggagataa aaaggatgaa
    22981 ggagcagccg ttcttcagtc catccatgtc tatccgatgt tcggtgagcc atatgaggta
    23041 atcgtcgaac aagaagcggg cggacatggc ggaggagatc cgcgtttatt agatgatcta
    23101 tttggtgaag aaaaagaaga agatccgtgg aatcgggcag caactcacgt agatggtatt
    23161 ttgtcgattt tgacaggaat cgcagcaaat cattctattg cttcaggaaa agcagtagtg
    23221 attgacgagc ttgtcagctt aaaagaataa gaatagattt acggaaatta cttcccgtag
    23281 tttataaaat cttgccttat tgccgatagg ctgtcttttg aacactattt attcggaaaa
    23341 aggggctatg acatgctttt tgtcatgcct tctttttatg tgggctgctt ttttagatag
    23401 gagaaaataa aaataactat ttaaacccga acaaaaagga ataatgcatt aaaaatcatt
    23461 agattttaca aaaaaataag taaaccacgt tgtcagtttc agaaagtagt gttagactgt
    23521 tttcaatatt tgaaacaaca ttttttattc aaatatggca aactgccttc acgtgcaaaa
    23581 cagttgcaag aaaatgaatt tacactatta ttagttttac atacctactt atatatcgtc
    23641 ataatatgga tgacagtttc tagccagtac cgtaaatgct ggactataag taaaagattg
    23701 gctatttcac ccttcttttc ttatgataaa gtggagggtg tttttcttta acagaaacat
    23761 agtaggtttt tgcacaaaag cggttatttc ggtattaata aatgataaag gagcaaataa
    23821 agtgaaagaa ttagttgaac gtattcaaaa agacggacgt atattaggcg agggcgtatt
    23881 aaaagtcgac agcttcgtca ctcaccaagt agaccctgta ttgatggaac aaatgggaaa
    23941 acggtttgca gaagtattta aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc
    24001 tgggatcgca ccagctttat ttgctgcaag agaattagat gttccaatga tcttcgctag
    24061 aaaagcaaaa agtttgacga tggatgaaga attattgaca gcttctgttt attcttttac
    24121 gaaacaagtg acaagtacga tttctatttc tcgtaaattt ttatcttcag aagacaaagt
    24181 attgattatc gacgatttct tggcaaacgg acaagcggca aaaggattga tcgaattatg
    24241 ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga
    24301 tggacgtcag ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc
    24361 cttgacgaat ggacaagttg agtttcttga ggaggacgca taatcgtgga aaaagaaaca
    24421 caaaatggaa aagctgcggt tttagggctg cagcatctgc tagcgatgta tgcaggagca
    24481 gtagcggtgc ctcttttgat cggtacagga ctaggcttta atcaagaaca gatgacttat
    24541 ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat tattgcaatt gacagttaac
    24601 cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca
    24661 ctgatcttga tcggaaccaa tgaaggagta ggagctatct acggatctat tatcgcttct
    24721 gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact attccctcct
    24781 atagtaacag gaacggttat caccgttatt ggtttgactt tgattcctgt agcaatcgag
    24841 aaaatgggtg gaggtaatgc aacagcagct gattttggtg acaaaacaaa tctgctactt
    24901 gcatttgtga cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt
    24961 tcaatcgcgg tattgattgg tttagtcgga ggaagcatct tagctgcttt catgggaatg
    25021 attgatctga gcgcgattgg agatgcgccg ttgttccatg ttccacaacc cttttatttt
    25081 ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga tcatcatttc catggtcagc
    25141 atggtagaat caacaggtgt atattttgca cttggtgaca ttactgggaa aaatgttgga
    25201 gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt aggcggtatt
    25261 ttcaatacat tcccttatac tggcttttct caaaatgtcg gattggttca actttcggga
    25321 atcaaaacaa gaaaacccat ctatttctct gcattcttct tgattgtttt aggattattc
    25381 cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg tacttggtgg aggaatgtta
    25441 gtcatgttcg ggatggtggc agtccaagga atccgtatgc tgttggaagt tgatttcagc
    25501 aatgataaaa atctattgat tgttgctgtt tctgttggat ttgggctggg tttcaatgtt
    25561 acgccgacat tattcagtca aatgccgcaa acattgcaaa tgttcactgg aaatggcatc
    25621 gtaatgagta gcattacggc tatcatattg aatttgatct ttaatggatt gaaaaaagat
    25681 gaagaaatat tggaagaagc cttcaagaag taatagagca aaagaaaggt tgtgaaatca
    25741 gcgttttgac ctgattgtta gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt
    25801 tgtttgagct aataccgagg gtcagctgat tgaacaccgt tgaaggaaga ggtcgtgagc
    25861 ctgccgttca tctccgagtc acaaggaaca atttcaaaaa aaagcttctc atgtttgttg
    25921 aatattgttc cttgtgaccg aggagttggc aggagaacac cgtttatcca taaaaaggaa
    25981 tgtgacctaa cttttgtcac attccttttt tttatcttta ataaacacga acaataaaat
    26041 gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat
    26101 gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag
    26161 tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc
    26221 ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca
    26281 aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct
    26341 gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg
    26401 aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt
    26461 agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat
    26521 gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc
    26581 taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga
    26641 tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg
    26701 gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt
    26761 ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag
    26821 atgtcattac ctatgaattc gaaaacgtca gtatagaggc gttaaatgcg attttaccca
    26881 tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa
    26941 aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga
    27001 cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag
    27061 gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca
    27121 tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga
    27181 tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa
    27241 acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg
    27301 tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg
    27361 ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc
    27421 ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg
    27481 cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg
    27541 cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata
    27601 agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg
    27661 gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga
    27721 atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga
    27781 atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg
    27841 aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg
    27901 catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg
    27961 cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc
    28021 taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata
    28081 ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca
    28141 aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat
    28201 tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg
    28261 caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc
    28321 catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa atttctactc
    28381 aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa
    28441 gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag
    28501 aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga
    28561 tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct
    28621 atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca
    28681 tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta
    28741 aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga
    28801 tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt
    28861 atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc
    28921 tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct
    28981 ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat
    29041 aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa
    29101 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
    29161 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
    29221 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
    29281 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
    29341 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
    29401 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
    29461 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
    29521 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
    29581 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
    29641 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
    29701 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
    29761 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
    29821 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
    29881 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
    29941 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
    30001 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
    30061 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
    30121 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
    30181 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
    30241 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
    30301 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
    30361 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
    30421 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
    30481 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
    30541 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
    30601 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
    30661 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
    30721 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
    30781 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
    30841 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
    30901 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
    30961 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
    31021 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
    31081 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
    31141 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
    31201 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
    31261 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
    31321 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
    31381 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
    31441 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
    31501 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
    31561 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
    31621 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
    31681 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
    31741 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
    31801 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
    31861 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
    31921 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
    31981 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
    32041 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
    32101 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
    32161 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
    32221 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
    32281 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
    32341 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
    32401 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
    32461 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
    32521 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
    32581 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
    32641 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
    32701 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
    32761 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
    32821 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
    32881 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
    32941 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
    33001 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
    33061 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
    33121 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
    33181 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
    33241 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
    33301 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
    33361 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
    33421 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
    33481 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
    33541 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
    33601 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
    33661 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
    33721 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
    33781 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
    33841 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
    33901 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
    33961 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
    34021 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
    34081 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
    34141 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
    34201 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
    34261 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
    34321 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
    34381 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
    34441 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
    34501 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
    34561 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
    34621 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
    34681 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
    34741 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
    34801 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
    34861 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
    34921 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
    34981 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
    35041 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
    35101 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
    35161 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
    35221 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
    35281 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
    35341 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
    35401 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
    35461 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
    35521 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
    35581 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
    35641 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
    35701 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
    35761 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
    35821 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
    35881 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
    35941 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
    36001 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
    36061 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa
    36121 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
    36181 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
    36241 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
    36301 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
    36361 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
    36421 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
    36481 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
    36541 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
    36601 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
    36661 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
    36721 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
    36781 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
    36841 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
    36901 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
    36961 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
    37021 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
    37081 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
    37141 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
    37201 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
    37261 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
    37321 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
    37381 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
    37441 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
    37501 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
    37561 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
    37621 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
    37681 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
    37741 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
    37801 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
    37861 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
    37921 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
    37981 agccgactcc agcagaagca aaagcattag tgtttgcttg gagagctgtc aaacatgtga
    38041 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
    38101 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
    38161 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
    38221 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
    38281 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
    38341 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
    38401 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
    38461 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
    38521 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
    38581 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
    38641 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
    38701 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
    38761 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
    38821 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
    38881 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
    38941 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
    39001 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
    39061 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    39121 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    39181 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    39241 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    39301 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    39361 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    39421 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    39481 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    39541 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    39601 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    39661 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    39721 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    39781 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    39841 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    39901 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    39961 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    40021 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    40081 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    40141 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    40201 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    40261 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    40321 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    40381 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    40441 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    40501 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    40561 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    40621 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    40681 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    40741 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    40801 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    40861 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    40921 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    40981 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    41041 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    41101 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    41161 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    41221 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    41281 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    41341 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    41401 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    41461 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    41521 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    41581 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    41641 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    41701 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    41761 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    41821 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    41881 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    41941 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    42001 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    42061 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    42121 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    42181 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    42241 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    42301 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    42361 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    42421 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    42481 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    42541 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    42601 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    42661 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    42721 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    42781 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    42841 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    42901 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    42961 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    43021 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    43081 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    43141 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    43201 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    43261 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    43321 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    43381 gattgatgat ctcttcaatg gtatccataa aatcttttgc caagatatta ttgatcagct
    43441 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    43501 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    43561 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    43621 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    43681 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    43741 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    43801 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    43861 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    43921 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    43981 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    44041 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    44101 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    44161 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    44221 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    44281 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    44341 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    44401 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    44461 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    44521 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    44581 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    44641 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    44701 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    44761 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    44821 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    44881 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    44941 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    45001 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    45061 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    45121 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    45181 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    45241 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    45301 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    45361 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    45421 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    45481 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    45541 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    45601 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    45661 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    45721 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    45781 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    45841 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    45901 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    45961 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    46021 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    46081 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    46141 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    46201 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    46261 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    46321 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    46381 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    46441 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    46501 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    46561 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    46621 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    46681 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    46741 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    46801 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    46861 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    46921 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    46981 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    47041 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    47101 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    47161 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    47221 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    47281 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    47341 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    47401 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    47461 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    47521 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    47581 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    47641 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    47701 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    47761 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    47821 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    47881 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    47941 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    48001 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    48061 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    48121 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    48181 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    48241 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    48301 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    48361 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    48421 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    48481 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    48541 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    48601 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    48661 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    48721 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    48781 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    48841 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    48901 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    48961 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    49021 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    49081 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    49141 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    49201 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    49261 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    49321 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    49381 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    49441 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    49501 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    49561 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    49621 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    49681 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    49741 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    49801 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    49861 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    49921 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    49981 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    50041 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    50101 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    50161 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    50221 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    50281 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    50341 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    50401 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    50461 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    50521 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    50581 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    50641 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    50701 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    50761 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    50821 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    50881 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    50941 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    51001 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    51061 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    51121 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    51181 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    51241 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    51301 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    51361 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    51421 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    51481 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    51541 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    51601 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    51661 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    51721 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    51781 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    51841 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    51901 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    51961 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    52021 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    52081 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    52141 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    52201 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    52261 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    52321 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    52381 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    52441 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    52501 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    52561 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    52621 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    52681 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    52741 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    52801 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    52861 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    52921 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    52981 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    53041 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    53101 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    53161 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    53221 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    53281 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    53341 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    53401 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    53461 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    53521 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    53581 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    53641 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    53701 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    53761 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    53821 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    53881 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    53941 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    54001 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    54061 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    54121 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    54181 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    54241 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    54301 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    54361 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    54421 acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DACEKG010000008        52640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEKG010000008 DACEKG010000000
VERSION     DACEKG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.008"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..971
                     /locus_tag="IW664_000403"
     CDS             117..971
                     /locus_tag="IW664_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0847794.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="IW664_000404"
     CDS             972..2309
                     /locus_tag="IW664_000404"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0847795.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="IW664_000405"
     CDS             2314..2538
                     /locus_tag="IW664_000405"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0847796.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="IW664_000406"
     CDS             2540..3430
                     /locus_tag="IW664_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0847797.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="IW664_000407"
     CDS             3439..4257
                     /locus_tag="IW664_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0847798.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="IW664_000408"
     CDS             4364..4813
                     /locus_tag="IW664_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0847799.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="IW664_000409"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="IW664_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0847800.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="IW664_000410"
     CDS             complement(6568..6684)
                     /locus_tag="IW664_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0847801.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="IW664_000411"
     CDS             complement(6757..7713)
                     /locus_tag="IW664_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0847802.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="IW664_000412"
     CDS             7884..8252
                     /locus_tag="IW664_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0847803.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="IW664_000413"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="IW664_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0847804.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="IW664_000414"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="IW664_000414"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0847805.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="IW664_000415"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="IW664_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0847806.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="IW664_000416"
     CDS             10240..12432
                     /locus_tag="IW664_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0847807.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="IW664_000417"
     CDS             12458..13420
                     /locus_tag="IW664_000417"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0847808.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="IW664_000418"
     CDS             13434..14795
                     /locus_tag="IW664_000418"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0847809.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="IW664_000419"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="IW664_000419"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0847810.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="IW664_000420"
     CDS             15924..17144
                     /locus_tag="IW664_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0847811.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="IW664_000421"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="IW664_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0847812.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW664_000422"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW664_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0847813.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="IW664_000423"
     CDS             19906..20583
                     /locus_tag="IW664_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0847814.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="IW664_000424"
     CDS             20597..21196
                     /locus_tag="IW664_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0847815.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="IW664_000425"
     CDS             21211..21498
                     /locus_tag="IW664_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0847816.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="IW664_000426"
     CDS             21547..22329
                     /locus_tag="IW664_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0847817.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="IW664_000427"
     CDS             22350..23057
                     /locus_tag="IW664_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0847818.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="IW664_000428"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="IW664_000428"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0847819.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="IW664_000429"
     CDS             complement(26166..27692)
                     /locus_tag="IW664_000429"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0847820.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="IW664_000430"
     CDS             complement(27707..28354)
                     /locus_tag="IW664_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0847821.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="IW664_000431"
     CDS             complement(28538..28867)
                     /locus_tag="IW664_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0847822.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="IW664_000432"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="IW664_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0847823.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="IW664_000433"
     CDS             29788..30801
                     /locus_tag="IW664_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0847824.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="IW664_000434"
     CDS             30801..32078
                     /locus_tag="IW664_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0847825.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="IW664_000435"
     CDS             32141..34843
                     /locus_tag="IW664_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0847826.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="IW664_000436"
     CDS             34995..35312
                     /locus_tag="IW664_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0847827.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="IW664_000437"
     CDS             35342..35662
                     /locus_tag="IW664_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0847828.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35748..37208
                     /locus_tag="IW664_000438"
     CDS             35748..37208
                     /locus_tag="IW664_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0847829.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37276..37497
                     /locus_tag="IW664_000439"
     CDS             37276..37497
                     /locus_tag="IW664_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847830.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37528..37710
                     /locus_tag="IW664_000440"
     CDS             37528..37710
                     /locus_tag="IW664_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0847831.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37710..38123
                     /locus_tag="IW664_000441"
     CDS             37710..38123
                     /locus_tag="IW664_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0847832.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38246..39427
                     /locus_tag="IW664_000442"
     CDS             38246..39427
                     /locus_tag="IW664_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0847833.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            39816..39889
                     /locus_tag="IW664_000443"
     tRNA            39816..39889
                     /locus_tag="IW664_000443"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39850..39852,aa:Arg,seq:cct)
     gene            complement(39958..41097)
                     /locus_tag="IW664_000444"
     CDS             complement(39958..41097)
                     /locus_tag="IW664_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0847834.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41396..42031)
                     /locus_tag="IW664_000445"
     CDS             complement(41396..42031)
                     /locus_tag="IW664_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847835.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42144..42779)
                     /locus_tag="IW664_000446"
     CDS             complement(42144..42779)
                     /locus_tag="IW664_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ0847836.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42813..43274)
                     /locus_tag="IW664_000447"
     CDS             complement(42813..43274)
                     /locus_tag="IW664_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ0847837.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(43404..43835)
                     /locus_tag="IW664_000448"
     CDS             complement(43404..43835)
                     /locus_tag="IW664_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0847838.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(43853..44173)
                     /locus_tag="IW664_000449"
     CDS             complement(43853..44173)
                     /locus_tag="IW664_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0847839.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            44472..45248
                     /locus_tag="IW664_000450"
     CDS             44472..45248
                     /locus_tag="IW664_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0847840.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            45263..45466
                     /locus_tag="IW664_000451"
     CDS             45263..45466
                     /locus_tag="IW664_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0847841.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            45482..45820
                     /locus_tag="IW664_000452"
     CDS             45482..45820
                     /locus_tag="IW664_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0847842.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            45807..45986
                     /locus_tag="IW664_000453"
     CDS             45807..45986
                     /locus_tag="IW664_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847843.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(46029..46499)
                     /locus_tag="IW664_000454"
     CDS             complement(46029..46499)
                     /locus_tag="IW664_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847844.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            46586..47284
                     /locus_tag="IW664_000455"
     CDS             46586..47284
                     /locus_tag="IW664_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0847845.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            47462..47803
                     /locus_tag="IW664_000456"
     CDS             47462..47803
                     /locus_tag="IW664_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847846.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            47796..48467
                     /locus_tag="IW664_000457"
     CDS             47796..48467
                     /locus_tag="IW664_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0847847.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            48473..49159
                     /locus_tag="IW664_000458"
     CDS             48473..49159
                     /locus_tag="IW664_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847848.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            49162..49911
                     /locus_tag="IW664_000459"
     CDS             49162..49911
                     /locus_tag="IW664_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0847849.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            49923..50192
                     /locus_tag="IW664_000460"
     CDS             49923..50192
                     /locus_tag="IW664_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847850.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            50354..50656
                     /locus_tag="IW664_000461"
     CDS             50354..50656
                     /locus_tag="IW664_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847851.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            50653..50814
                     /locus_tag="IW664_000462"
     CDS             50653..50814
                     /locus_tag="IW664_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0847852.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            50811..51116
                     /locus_tag="IW664_000463"
     CDS             50811..51116
                     /locus_tag="IW664_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847853.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            51116..51472
                     /locus_tag="IW664_000464"
     CDS             51116..51472
                     /locus_tag="IW664_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0847854.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            51432..51677
                     /locus_tag="IW664_000465"
     CDS             51432..51677
                     /locus_tag="IW664_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847855.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            51674..52093
                     /locus_tag="IW664_000466"
     CDS             51674..52093
                     /locus_tag="IW664_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0847856.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            52090..>52640
                     /locus_tag="IW664_000467"
     CDS             52090..>52640
                     /locus_tag="IW664_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0847857.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVV"
BASE COUNT        17789 a         8801 c        10970 g        15080 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcg ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattattc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgcttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggattttt tattttgtta
    35701 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    35761 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    35821 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    35881 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    35941 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    36001 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    36061 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    36121 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    36181 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    36241 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    36301 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    36361 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    36421 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    36481 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    36541 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    36601 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    36661 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    36721 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    36781 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    36841 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    36901 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    36961 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    37021 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    37081 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    37141 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    37201 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    37261 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    37321 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    37381 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    37441 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    37501 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    37561 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    37621 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    37681 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    37741 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    37801 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    37861 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    37921 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    37981 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    38041 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    38101 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    38161 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    38221 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    38281 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    38341 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    38401 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    38461 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    38521 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    38581 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    38641 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    38701 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    38761 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    38821 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    38881 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    38941 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    39001 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    39061 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    39121 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    39181 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    39241 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    39301 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    39361 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    39421 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    39481 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    39541 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    39601 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    39661 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    39721 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    39781 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    39841 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    39901 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    39961 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    40021 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    40081 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    40141 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    40201 atatttgctg acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt
    40261 atgagggttt ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt
    40321 taagagatca atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt
    40381 ttttccccat tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc
    40441 gaaatcgata tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc
    40501 agcaactaac acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt
    40561 cattaaagat ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc
    40621 ttttcctttg attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc
    40681 atctttcaac gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc
    40741 tttagcatac ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac
    40801 cgctggcata tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg
    40861 agatactgtt ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag
    40921 atttttgcga tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc
    40981 ttttgcttct ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc
    41041 ttttttgtat gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat
    41101 tgaatcagct ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt
    41161 tattattttt tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta
    41221 atggtataat tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg
    41281 atttttaaaa gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc
    41341 tggtttttta ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag
    41401 taagagttga gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt
    41461 tgccgacatc acctaatact atggagctaa tcgttttttc taacagacca aaatcagata
    41521 atctatttaa gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag
    41581 tgaaattttc gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta
    41641 attgattcag tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa
    41701 gtaaacaatt cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt
    41761 ctacctcgtc atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga
    41821 aaacatcttc tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga
    41881 atttctcgat gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa
    41941 cgccaccaat ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt
    42001 ttttggttag tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta
    42061 caagcattgt gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta
    42121 actcttagtg gggcgtggtt catttatttt ttacttactt tactattaat catatcccat
    42181 tgagcatagg atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact
    42241 ttatcgccct ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa
    42301 actttaggtg ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata
    42361 atggctattc tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca
    42421 ctaatatatt tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta
    42481 tctaagaaag caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg
    42541 cttttattgc tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg
    42601 ttattgtcat ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc
    42661 ccgataataa ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt
    42721 aacatgatta aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt
    42781 aatagatcct ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta
    42841 aaatcgaatt cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt
    42901 tcatctgcac ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc
    42961 tctatatatt tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg
    43021 ttttcatctt tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta
    43081 aattgtatat aaccattact catacctggt ttttttaatt gtacggctga tatattttta
    43141 aaggggatag ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta
    43201 ataattgaaa tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa
    43261 atctcttttt ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca
    43321 tatatgagga aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt
    43381 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    43441 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    43501 tccataaatt tttcgagatt aaactgagat ttatcagtca gttcgttctc aatatagata
    43561 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    43621 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
    43681 tgagataaat gaaaggcttc tgcagtttca ccgtatagat tttcattcaa aaaaatgatt
    43741 ctgggttttg gatagtagaa accaggctct ttcatctcca tataaactac tttcaaattg
    43801 tattcgctca acatttcttt caatttcaaa tacatacaaa ccatcactcc aactattcat
    43861 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    43921 ttccatcaaa agaaaaaaca gtatcgtctt ctgataaatc cacatgttta ggggtttctc
    43981 tttcttctct acctagaagg tagtctacag agacatcgaa atagtcagca atccgactta
    44041 attcatctga attaggagta ttatttttcc actttgctag ataaccgtta gaatatccaa
    44101 aattaatttc taactgccgt atagatatct ttttcctctt cgccaagtct tttattattt
    44161 cataggtatt catcgataaa acaacctttc taaatgttta caaaaaaagt ttagaaaaat
    44221 acgcagaaat aatttgacta attctgagta atgagctata ctatatcttg taaacagatt
    44281 taacaactaa aaagacaaca aaaaacacta ttgatatgta aatgcagacc gccaagaaag
    44341 ctttaaaatc aatgttttta tgtcttattt aattatgctc ttagtataga gtattactca
    44401 gatactgtca attgaattta gaaaaaagtt gttaaatttg tttacgaata taaaagaaag
    44461 gaatgagaaa tatgaacaca ccgcaaattt ttaatttcga acaaaacgaa gttcggacaa
    44521 ttttagtaaa tgatgaacca tattttgtag gcaaagacgt tgcgagtgtt ttgggttatt
    44581 caaacactaa agatgctttg tcgcggcatg tagatttgga agataagatg gggtcgcgaa
    44641 tcacgacctc aggtcaatca agagagatga caatcatcaa cgaatctggt ttgtacagtt
    44701 taatcttaaa atcaaaactt ccctctgcca aaaaatttaa acgttgggta acaagtgaag
    44761 tgttgccagc aattagaaaa catggaggtt atctaactcc agaaaaagta gaagaagctt
    44821 tgcttaatcc agatacaatc attcaattag caactcaact aaaagaagaa agaactggaa
    44881 gattaatcgc agaacaaaag attgcagagt acgaacctaa aatctcctat ttagatagca
    44941 tattatcttc tacagattca gtaacaatta gtcagattgc agcagattat gggatgtctc
    45001 cacaacagat gaataaatta cttcataaac taggtattca gaaaaaagtc ggtaaccaat
    45061 ggttattgtg caaaaaacac atgaaccaag gatacacaaa atctcataca actgagatcc
    45121 cgaaagccga tggtggcact aaaattgtaa tgaataccaa atggacacag aaagggcgtc
    45181 tatttatcta cgaattacta aaaaaagaag gatattaccc tcaaatggat ttagaggaaa
    45241 ttggttagaa aggagtttta gtatgactga cattgcagaa atcactcaac gagatagaga
    45301 aaaaatcaaa gaatatgtcg aaagttcaaa gttcttaact tacaccatgc ttgctgaaag
    45361 atttggaatt agcaaaagct acttatcttt aattttaaac ggtaaaaaga cttctgcaga
    45421 agcaaacaga attatagatt cgattatcac tatgtacgaa ttgtaaaggg aggaacagct
    45481 aatgcaatat ctagaagcaa aaatcccaat tccagaaggc tatgtaatta tctcccaagt
    45541 ggattatgag gagttaaaaa aagctgatga tactggtaga tggatgacgt tgccagaagt
    45601 gctagaacgg attaacagaa aatatgattg gtttacttct agagttttaa agaacccaag
    45661 atatagaaat attatcgata tcgagaaaaa caaaaatgga tttgtctatt atccagttga
    45721 aggaagagac acatatctat ttttaagaag taaaacactt gaatttttag aaacaaattt
    45781 ttcggaaatc ttaaggaggc aagcggatgg gaaaatttaa tagagcgcta gtgttcagcg
    45841 caccgctaat catctacgct ttaggacttt ggggaagcag gcaagcgttg ataggaacga
    45901 ttgtttacat ggtttggatt tttatggggc ttgatgaagc tgaagctgag tacagagcga
    45961 aaaagccaac cgagggggct gactaaaaat gaatagaaaa gagaaactag aatgtatatt
    46021 actattactc agtttaattc tttcactaat ttctctattg ggaagtttgt atttttgata
    46081 tcaacaattt tgtggatttg ggttctattt agagtaaata tcatttttgt ttggtgatta
    46141 aggagtttat taaaattaat tttccctact tcaaatgcaa ggtaacaaga tatagcactt
    46201 ttaggaggaa tatttagcgg tacagcttca gagtatattc tattatcatc gactgatcct
    46261 cctttagcaa ttaatttctt aaatggagta gctgaccatc tgcggtttaa gttagcggat
    46321 gaaagttcta aattaactaa catagctggt tctgttgaat agttagaaat gattactttt
    46381 gtataaatca tatctgcagc gaagtaagaa gcattaagtt caacttctag ttgagggcgc
    46441 attcctttta tttttatccc tgttaaaaac gtactcaata taaaacttat tattgacatc
    46501 cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt ttaactaaat
    46561 tataccaaaa aggagagaag aaataatgca agaattagta attttgaaaa ataaagaagc
    46621 tgtgactacg agcttacaag tggcagaaag cttcgagaaa aaacatcaac atgttttaag
    46681 agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttg tagaaggtaa
    46741 tgaaccagat tcatatggca gaaatcgacg agttttcttc attagtagag atggattttt
    46801 cttgctggct atggggttta caggaaagaa agctatctac ttcaaacaaa aatacattga
    46861 agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga
    46921 tatgatgatc tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa
    46981 agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa
    47041 agttatggga gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt
    47101 attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt
    47161 acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttgc cagaaactgc
    47221 gatccgtatg gaaattgatc aactgaacag acaacagaga ctttttggtg atgacaatga
    47281 atagagctga agcgctaaga atagggacgg caattgctaa ttgctggtgg aaatactata
    47341 aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa caaataaaaa
    47401 agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca
    47461 tatggaaaaa gaactttcca ctctagatca atatttgatt gatcctgatt ggggcaagcc
    47521 gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga atgaagaact
    47581 agcttgggat caagatgatt taggcaacca tgtaactatt tgggatcatg tttatcttat
    47641 ccatctatcg aagcattcga ataaacctga atatatttac gtcatcgaag atggcttgat
    47701 tgatgcgcta gaagagtacg acagagataa cttgattgat atctcttatt acggaccagg
    47761 taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaatcaaa gggacaacga
    47821 actttgaaaa actttttagt cgtaagttaa ataaaattct caagaaaaaa ggaaattttg
    47881 attatttatc ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg
    47941 taactattaa tgagtacaaa cactacaggg ttgtttctgg aactcatcaa gactttcttg
    48001 ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct gtctcagtaa
    48061 cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga aaccaaccag
    48121 ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt gtgaaagcat
    48181 tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca acaccaccta
    48241 gaatcgatac aaagaaatta aacatgctag agacgattct agaagctttc aatgagcaga
    48301 tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca gataaattag
    48361 ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa tgtgccttga
    48421 tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat gagtgtttaa
    48481 accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag acataagcgc
    48541 aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg gctacgatgg
    48601 tgatcgagtc attgaaattc gttttattga tcctagacgt ttcaccgtac agcaacgaaa
    48661 cttcatctat gcgctgatag gcgatatttt tatcgataca ggcatgccaa cggacttctg
    48721 gaaggaattc ttctactttc gttttgaagg tgtcacaggg cgcaaaataa gcctgaaaga
    48781 cgaatcgaat acaactgtga gtgatgccaa tgtcttagca aatatcatct tagatttcat
    48841 ctttgaacat catattcctt tcaaagaagg ctatgagatt ttacctgcga atcaagaata
    48901 ttacttctac aaatgcatca caaaaagagt ctgctgcatc tgtggcaaaa caggagctga
    48961 catcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcatgcaga
    49021 gtacacattt gcagcactct gcagaatcca tcacacagag aagcacaaaa taggtgtgat
    49081 taatttcaaa aataaatatc aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa
    49141 gttaaatatt ggagggtaaa aatgacagaa catcgaagtt attatgcgat tataccagcc
    49201 aacgtaaggt acgacaaaag acttaaacca aatactaagt tgttatacgg agagataacg
    49261 gccttgtgta atgaaagagg cttttgttgg gcaggcaatg agtactttgc agatttatat
    49321 ggtgtgaata aagagaccat atcgcgatgg gtaagtgatt tgattaagtt tggatacttg
    49381 aatcgggaaa tcatttacaa agagggtacc aatcaaataa tcaataggta cctacgaatt
    49441 aatcaatacc ctattgacga aaaacgcaat acccctattg acgaaaaagt caaagataat
    49501 aatacatcta ttaataatac atttaataat acaaaagaat atataagaga gttaccgcct
    49561 tcgaaaaaat cgaaggctaa gcccgtccgt cataaatacg gagagtataa aaatgttctt
    49621 ttgtcagatg agcagatgga gaaactcaaa acagaattcc ctaatgatta ccaagagcga
    49681 atcgaacgac tgtcagagta ttgtgaatca tctggtaaga cttataaaaa ctatttggca
    49741 actattcgaa gttgggcaag gaaagaaaaa agtgaaccta agaacgcaag cagtggatac
    49801 aagcgcacag gaagacgaga gaagcttcct gaatgggcaa tcgaccaaga agcctatctc
    49861 aagaaaaaag cgctagaacg agctaataga caatcaaaag caccattcta agaggtggaa
    49921 aaatgaagat cgattatcta gaactaatta atgaaatagc gaattataaa aagggcgagg
    49981 aattagacgt cctgagagac gtatatgatc aactcgaaga agctggaatt gaaggaatta
    50041 agaatgatcg ttcgagttgg agtaagctca gatactattt cgcactttat atcgatacaa
    50101 cacaattaag aaatttagca tataccaaat tactatttgt tgattgtgtt aaaggcttgc
    50161 aaaaacatct taatgaactt gagcaggtgt aatcagatgg acctaaagac atttacagca
    50221 cagattgaac taatgcatca agaagcttta agacaaagtg tatcgtacga agacaagtgg
    50281 ctcaacacgt tccatggcgg acgtgagagc gcacttgatc aagtactcaa attactgaaa
    50341 ggagaatgtc gggatggata agaaagcggc aatgcagcga attatcgaat tgacttattc
    50401 agaagattgg caaaatgaca aagaagctgc ttcagaagtg atgaggcttg gaagagcgat
    50461 gtgggcagac aagagcaaca agccaagacc acgaaaaatc gcaatttggc acggtgacaa
    50521 acttctagtg atagggacag ctgaacagtt agcaagtctc acaggcttgc acgagaaaat
    50581 cgtgaggaaa agagcaaggt gtggctacac agacgctaag aagagaacgt ttagatacgt
    50641 ggaggaatca tcatgacaac agaagaagtg attcaaatgc gtattcgaag ccttcagcgt
    50701 gagattgacg atctggaacg aacaaaggca gtgatggtca atgaaacggc gagaaaggca
    50761 atcgatttgc acatagagaa tttaagaagg gaaatccatc gattggagga atgagcgtgg
    50821 ataagaaagc agcaatgaaa agaattgctg aattaaccaa gtcagaatct tggcaagaag
    50881 acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca
    50941 aacggagaac gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta
    51001 ctgctgaaca gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta
    51061 ggagcttatg gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga
    51121 tctcattaca caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga
    51181 taaaaaatca aaagaacgag ctgaattagt taagttagaa atggctgaaa caggagcagg
    51241 agtgcgaagt agcaggcatt ggaaagcagc agcaaacatt gaattttatt acaacgaaat
    51301 tcaaaaaggg ttcgatcaga tgcgtgagct ggatcggcaa acaaattgga gcaagaaact
    51361 tcatcaagat cgtttcaaat ttgtagagaa gtataaagaa atattagaag agtatttgag
    51421 gaggacagca aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat
    51481 caggagcaac actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc
    51541 agattatttt gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag
    51601 aatcaaaagc ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg
    51661 aggaggacag cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag
    51721 agatgtagcc gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat
    51781 aaatcctacc gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg
    51841 aatgaaagat aagaatggtg tggatatatt tgaaggggat atagtattag tcagcgtgcg
    51901 aaatggcttc gattacttag ataataaagt ctgtattgtc aaaaattcaa tagattattc
    51961 cggattagtt tgtgccactg ttgatgaaga cttagagtat caaattttta acacagagct
    52021 gtttgaagaa tacacgtacg aagtcaccgg aaatatatac gagaatagcg agttattgga
    52081 ggaacagcga tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agcgatagga
    52141 aatgatgcaa tagctgcttg ctataacgag agtataaatt caggtattac gttaatgaaa
    52201 aaaatagacg aaccgcagaa accagttatt ccacaactcg tggccggttg gcttgagaaa
    52261 tctacggacc cttttacaaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt
    52321 gattcatatt atttctgtga ttggtttgta cgagatggca tagtgacgca agagcaagga
    52381 gaagaattac tcgcttgggc aacaagacaa tcatatgaaa cactattgag cctatacaac
    52441 ggctacgagg ttgagaaaga gccgttatgg gcaataaaga atgccgatgg aaactatctt
    52501 actaaatgtg ctttatgggg aaaagatgga gtaaattata gttttgaatg caatccatct
    52561 catcgattgc ttttcactga taaagcaaca gcggatgctg cagcattgtt ggtgaatgga
    52621 acagtggaag aggtggtaga
//
LOCUS       DACEKG010000009        52101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEKG010000009 DACEKG010000000
VERSION     DACEKG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.009"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            290..1135
                     /locus_tag="IW664_000468"
     CDS             290..1135
                     /locus_tag="IW664_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0847858.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IW664_000469"
     CDS             1416..2636
                     /locus_tag="IW664_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0847859.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IW664_000470"
     CDS             2633..5353
                     /locus_tag="IW664_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0847860.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IW664_000471"
     CDS             5337..6281
                     /locus_tag="IW664_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0847861.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IW664_000472"
     CDS             complement(6350..7360)
                     /locus_tag="IW664_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0847862.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IW664_000473"
     CDS             complement(7540..7884)
                     /locus_tag="IW664_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0847863.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IW664_000474"
     CDS             complement(7877..8311)
                     /locus_tag="IW664_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0847864.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IW664_000475"
     CDS             8449..9186
                     /locus_tag="IW664_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847865.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IW664_000476"
     CDS             9179..10390
                     /locus_tag="IW664_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0847866.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IW664_000477"
     CDS             10597..11376
                     /locus_tag="IW664_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0847867.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IW664_000478"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IW664_000478"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0847868.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IW664_000479"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IW664_000479"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0847869.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IW664_000480"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IW664_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0847870.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IW664_000481"
     CDS             14978..16027
                     /locus_tag="IW664_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0847871.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IW664_000482"
     CDS             16024..16302
                     /locus_tag="IW664_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0847872.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IW664_000483"
     CDS             16277..16747
                     /locus_tag="IW664_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0847873.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IW664_000484"
     CDS             16764..17066
                     /locus_tag="IW664_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847874.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IW664_000485"
     CDS             17083..17454
                     /locus_tag="IW664_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0847875.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IW664_000486"
     CDS             17426..17785
                     /locus_tag="IW664_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847876.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IW664_000487"
     CDS             17957..18964
                     /locus_tag="IW664_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0847877.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IW664_000488"
     CDS             18989..20173
                     /locus_tag="IW664_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0847878.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IW664_000489"
     CDS             complement(20610..22193)
                     /locus_tag="IW664_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0847879.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IW664_000490"
     CDS             complement(22771..22914)
                     /locus_tag="IW664_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0847880.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="IW664_000491"
     CDS             complement(23136..23615)
                     /locus_tag="IW664_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0847881.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="IW664_000492"
     CDS             complement(23688..23972)
                     /locus_tag="IW664_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847882.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="IW664_000493"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW664_000493"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="IW664_000494"
     CDS             complement(24316..25602)
                     /locus_tag="IW664_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0847883.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="IW664_000495"
     CDS             25740..26411
                     /locus_tag="IW664_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0847884.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="IW664_000496"
     CDS             26377..26865
                     /locus_tag="IW664_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0847885.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="IW664_000497"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="IW664_000497"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0847886.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="IW664_000498"
     CDS             27826..28590
                     /locus_tag="IW664_000498"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0847887.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITKITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="IW664_000499"
     CDS             28823..31036
                     /locus_tag="IW664_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0847888.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /locus_tag="IW664_000500"
     CDS             31047..31493
                     /locus_tag="IW664_000500"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0847889.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="IW664_000501"
     CDS             31664..32923
                     /locus_tag="IW664_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0847890.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="IW664_000502"
     CDS             complement(33012..33959)
                     /locus_tag="IW664_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0847891.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /locus_tag="IW664_000503"
     CDS             34353..35645
                     /locus_tag="IW664_000503"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0847892.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="IW664_000504"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="IW664_000504"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0847893.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="IW664_000505"
     CDS             37538..38413
                     /locus_tag="IW664_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0847894.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="IW664_000506"
     CDS             38679..40562
                     /locus_tag="IW664_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0847895.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="IW664_000507"
     CDS             complement(40637..40921)
                     /locus_tag="IW664_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0847896.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="IW664_000508"
     CDS             complement(40911..41645)
                     /locus_tag="IW664_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0847897.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="IW664_000509"
     CDS             41774..42397
                     /locus_tag="IW664_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0847898.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="IW664_000510"
     CDS             42589..43710
                     /locus_tag="IW664_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0847899.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="IW664_000511"
     CDS             43717..46845
                     /locus_tag="IW664_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0847900.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="IW664_000512"
     CDS             46864..50058
                     /locus_tag="IW664_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0847901.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="IW664_000513"
     CDS             complement(50204..50911)
                     /locus_tag="IW664_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0847902.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51676..52074
                     /gene="spxA"
                     /locus_tag="IW664_000514"
     CDS             51676..52074
                     /gene="spxA"
                     /locus_tag="IW664_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0847903.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17097 a         9065 c        10780 g        15159 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacgaagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    51181 taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta
    51241 tagtaaattt tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat
    51301 aagcgttata ataaagaaca gtattattta aaatgacaga aaacattact attaaagaaa
    51361 taaaaaacat aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaataatag
    51421 tacagtttta tacgctgttt catatgttta gaaaatttga attagatttg aaaactctaa
    51481 tttttaaatg ataaaaaaag cgagaatgct ggttttactg tatattaacc ttagataact
    51541 ttcatttttt taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa
    51601 tagaaacaga tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa
    51661 aaggagtggt gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag
    51721 gctcgtgcgt ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa
    51781 ccattgaaca gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa
    51841 atcatctcga cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg
    51901 ttacaagaat tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg
    51961 attgatgata aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg
    52021 cgcgatgtac gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat
    52081 aatacgaaac ttggaaaaga c
//
LOCUS       DACEKG010000010        51561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEKG010000010 DACEKG010000000
VERSION     DACEKG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.010"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            350..1453
                     /locus_tag="IW664_000515"
     CDS             350..1453
                     /locus_tag="IW664_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0847904.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1737..2417
                     /locus_tag="IW664_000516"
     CDS             1737..2417
                     /locus_tag="IW664_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0847905.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2507..3541
                     /locus_tag="IW664_000517"
     CDS             2507..3541
                     /locus_tag="IW664_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0847906.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3555..3890
                     /locus_tag="IW664_000518"
     CDS             3555..3890
                     /locus_tag="IW664_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847907.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3874..6354
                     /locus_tag="IW664_000519"
     CDS             3874..6354
                     /locus_tag="IW664_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847908.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6410..7969
                     /locus_tag="IW664_000520"
     CDS             6410..7969
                     /locus_tag="IW664_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0847909.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7966..9330
                     /locus_tag="IW664_000521"
     CDS             7966..9330
                     /locus_tag="IW664_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0847910.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9501..10571
                     /locus_tag="IW664_000522"
     CDS             9501..10571
                     /locus_tag="IW664_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0847911.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10586..11254
                     /locus_tag="IW664_000523"
     CDS             10586..11254
                     /locus_tag="IW664_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847912.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11274..11852
                     /locus_tag="IW664_000524"
     CDS             11274..11852
                     /locus_tag="IW664_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0847913.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12255..13166
                     /locus_tag="IW664_000525"
     CDS             12255..13166
                     /locus_tag="IW664_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0847914.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13422..14339)
                     /locus_tag="IW664_000526"
     CDS             complement(13422..14339)
                     /locus_tag="IW664_000526"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0847915.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14541..14978
                     /gene="zapA"
                     /locus_tag="IW664_000527"
     CDS             14541..14978
                     /gene="zapA"
                     /locus_tag="IW664_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0847916.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15036..15584
                     /locus_tag="IW664_000528"
     CDS             15036..15584
                     /locus_tag="IW664_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0847917.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15699..18059
                     /locus_tag="IW664_000529"
     CDS             15699..18059
                     /locus_tag="IW664_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0847918.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18146..18460
                     /gene="trxA"
                     /locus_tag="IW664_000530"
     CDS             18146..18460
                     /gene="trxA"
                     /locus_tag="IW664_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0847919.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18642..20420
                     /gene="uvrC"
                     /locus_tag="IW664_000531"
     CDS             18642..20420
                     /gene="uvrC"
                     /locus_tag="IW664_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0847920.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20565..21464
                     /locus_tag="IW664_000532"
     CDS             20565..21464
                     /locus_tag="IW664_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0847921.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21457..22719
                     /locus_tag="IW664_000533"
     CDS             21457..22719
                     /locus_tag="IW664_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0847922.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22882..24780
                     /locus_tag="IW664_000534"
     CDS             22882..24780
                     /locus_tag="IW664_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0847923.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25079..25798)
                     /locus_tag="IW664_000535"
     CDS             complement(25079..25798)
                     /locus_tag="IW664_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0847924.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26135..26467
                     /locus_tag="IW664_000536"
     CDS             26135..26467
                     /locus_tag="IW664_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0847925.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26454..28457
                     /locus_tag="IW664_000537"
     CDS             26454..28457
                     /locus_tag="IW664_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0847926.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28476..28946
                     /locus_tag="IW664_000538"
     CDS             28476..28946
                     /locus_tag="IW664_000538"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0847927.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28967..29551
                     /locus_tag="IW664_000539"
     CDS             28967..29551
                     /locus_tag="IW664_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0847928.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29566..30552
                     /locus_tag="IW664_000540"
     CDS             29566..30552
                     /locus_tag="IW664_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0847929.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30542..30853
                     /locus_tag="IW664_000541"
     CDS             30542..30853
                     /locus_tag="IW664_000541"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0847930.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30875..32656
                     /locus_tag="IW664_000542"
     CDS             30875..32656
                     /locus_tag="IW664_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0847931.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32649..34025
                     /locus_tag="IW664_000543"
     CDS             32649..34025
                     /locus_tag="IW664_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0847932.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34032..34667
                     /locus_tag="IW664_000544"
     CDS             34032..34667
                     /locus_tag="IW664_000544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0847933.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34697..36052
                     /locus_tag="IW664_000545"
     CDS             34697..36052
                     /locus_tag="IW664_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0847934.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36244..37638)
                     /locus_tag="IW664_000546"
     CDS             complement(36244..37638)
                     /locus_tag="IW664_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0847935.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37730..38701
                     /locus_tag="IW664_000547"
     CDS             37730..38701
                     /locus_tag="IW664_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0847936.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38698..39480
                     /locus_tag="IW664_000548"
     CDS             38698..39480
                     /locus_tag="IW664_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0847937.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39649..40020)
                     /locus_tag="IW664_000549"
     CDS             complement(39649..40020)
                     /locus_tag="IW664_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0847938.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40154..40597
                     /locus_tag="IW664_000550"
     CDS             40154..40597
                     /locus_tag="IW664_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0847939.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40640..41449)
                     /gene="yidA"
                     /locus_tag="IW664_000551"
     CDS             complement(40640..41449)
                     /gene="yidA"
                     /locus_tag="IW664_000551"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0847940.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41468..42835)
                     /locus_tag="IW664_000552"
     CDS             complement(41468..42835)
                     /locus_tag="IW664_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0847941.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42819..43649)
                     /locus_tag="IW664_000553"
     CDS             complement(42819..43649)
                     /locus_tag="IW664_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0847942.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43812..44249
                     /locus_tag="IW664_000554"
     CDS             43812..44249
                     /locus_tag="IW664_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0847943.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44325..44945
                     /locus_tag="IW664_000555"
     CDS             44325..44945
                     /locus_tag="IW664_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0847944.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45134..45640
                     /locus_tag="IW664_000556"
     CDS             45134..45640
                     /locus_tag="IW664_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0847945.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45657..46556
                     /locus_tag="IW664_000557"
     CDS             45657..46556
                     /locus_tag="IW664_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0847946.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46643..47527
                     /locus_tag="IW664_000558"
     CDS             46643..47527
                     /locus_tag="IW664_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0847947.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47719..47790)
                     /locus_tag="IW664_000559"
     tRNA            complement(47719..47790)
                     /locus_tag="IW664_000559"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47755..47757),aa:Arg,seq:ccg)
     gene            47850..49184
                     /gene="rpoN"
                     /locus_tag="IW664_000560"
     CDS             47850..49184
                     /gene="rpoN"
                     /locus_tag="IW664_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0847948.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49387..50424
                     /locus_tag="IW664_000561"
     CDS             49387..50424
                     /locus_tag="IW664_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0847949.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50465..51466
                     /gene="gap"
                     /locus_tag="IW664_000562"
     CDS             50465..51466
                     /gene="gap"
                     /locus_tag="IW664_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0847950.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        17045 a         8919 c        10915 g        14682 t
ORIGIN      
        1 ttgaacaccg ttttatcgag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa
       61 attttgtgaa aatggctttc cacatttttg cataaatttg acttaatacc gagaaaatgg
      121 cttttgaaca ccgtttattc aaaaaaaggg actttgacac tacttttgtc aaagtcccat
      181 cggtttttta tctattttta ttacaatcat ttcttccaga gtagtgacat ctggaatggc
      241 tgaaatgtaa acagattatt tgcctgtatc aatgtcgaag agtaatataa aaagtgtaaa
      301 cgctatcata ttgttcatca ttcaaatttt atagagagaa ggcattgaga tggaaaaaac
      361 atatcaagca ggtacaaatg aaggaatagt agattttatc aatatggaag atttagaaat
      421 cgcggcttca caagtgattc cagctggtgg ttatggttac atcagcagtg gtgctggcga
      481 cttatttact tatcaagaaa atgaacgggc attcaatcat cgtttgatta ttcctcatgt
      541 attaagagat gtagaattac ccgatacaac tacacatttt gacgaagaaa tgctgactgc
      601 accaatcatc atggcaccag ttgctgctca tggattagct catgtgaagg cggagaaagc
      661 ttctgcaaaa ggtgtagcag attttgggac gatctataca gcaagttctt acgcttcttg
      721 tactttagaa gagataagag aagcaggcgg ggagaaagca ccgcaatggt ttcaatttta
      781 tatgagcaaa gataatggaa tcaatttgga tattcttgaa gtggccaagc gtaatggagc
      841 aaaagcaatc gtattgacag cggatgcaac agttggcgga aaccgtgaaa cagaccgcag
      901 aaacgggttt accttcccgc tgccaatgcc aatcgttcag gcttatcaat caggagtagg
      961 ccaaacgatg gatgctgtat ataaatcttc caaacaaaaa cttagtccaa aagatgtaga
     1021 atttatcgct gctcattctg atttgccagt ctacgtgaaa ggtgttcaat cagaagaaga
     1081 tgtctatcga tcattagaat ctggtgctgg tggaatttgg gtatctaacc atggtggccg
     1141 tcaattagat ggggggcctg ctgcgtttga ttcgttgcaa tatgtggcag aagcagtaga
     1201 taaacgtgta ccaatcgtgt ttgacagcgg tgtacgacgt ggacaacatg ttttcaaagc
     1261 aatcgcatca ggtgccgatc ttgttgctat tggcagaccc gttatttatg gattgtcttt
     1321 aggaggaagt acaggagtcc atcaagtgtt tgatttcttt aaaacagaat tggaaatggt
     1381 catgcaatta gctggaacac aaacagtaga agatatcaaa aaaataaaat tgcgtgagaa
     1441 tcgttttatt taagaaataa atgacgaaaa aaataggagt gcggcagatg atgccgtact
     1501 cctattttta tagttcaagg atgtcactaa tagaataatt tgaaacgaaa aaataaaatt
     1561 gaaatatttt ttcatactta ataaaaaagg aagaatattc atgtaaacga ttttattgtt
     1621 tttttctctt ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat
     1681 tttttgcata ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga
     1741 aaaagaacat gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac
     1801 cagcttttgc tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa
     1861 ataccgttga gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg
     1921 aaaatccagc aactggccag acaggttctt taacattgga tgtagttcct gaattgccat
     1981 ttggtacaca cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg
     2041 atacacctta tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag
     2101 ggtggaacgt gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg
     2161 ctgaattaga ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac
     2221 caactagtca aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag
     2281 cagcaaaaga tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac
     2341 tgaaagtgcc gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg
     2401 ctggcccagt agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg
     2461 tgaaggattt tttaccgcag ttgttgttga aaggatgtgg tggacaatga atcaaagtaa
     2521 acgactgttt gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag
     2581 ccaagcaaag gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat
     2641 agataaagca atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga
     2701 agtaattatc agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc
     2761 ggtaacgagt gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac
     2821 attgccgttt gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc
     2881 acatgcagaa acaactatac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg
     2941 tgtattagcg ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga
     3001 aggagcacaa atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca
     3061 agagacgata gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa
     3121 gccaactgtt cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt
     3181 tacgagcaag atcttttatg aaaatcagct ttatattgaa aatacgtcta atgaattttt
     3241 agttgcacca aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc
     3301 cggcgattac cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac
     3361 acaaaacttt acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc
     3421 agtacaagaa caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt
     3481 atggttgatt ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg
     3541 atgaggtgat ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt
     3601 tcttcagctg gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt
     3661 atgatctaag tccagttccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga
     3721 cacagctacc gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaag
     3781 aaaagatccg ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt
     3841 tgttatggag aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt
     3901 tttcgctcct tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg
     3961 gcagaagctg ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat
     4021 atcgtacctc caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact
     4081 ggggctaacc aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct
     4141 tggaaaggtg gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt
     4201 gatttggtga atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa
     4261 catgtatcaa gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt
     4321 gccggtgccc ccatcaaggt tactagcggt agtttattat cagcaggaag ccataccttg
     4381 cgttttggga tggggtcgtg ggatacaaat actctattgg cagatattat tgctattatt
     4441 acgcgaggcg aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc
     4501 gcgaagatga cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag
     4561 ggtaaattac aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg
     4621 aataatatta tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt
     4681 tatcctaaat taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca
     4741 gagataaata cgtggaatag ttacatctca acaagtgatt caacaggaaa atataataca
     4801 tttgctgata ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat
     4861 agtcggacat tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc
     4921 tttgatcgcg tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact
     4981 catactccag caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa
     5041 gatgcgcaag gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta
     5101 gacggaacgg ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct
     5161 tctgcatcgt accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc
     5221 cattacaggc tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt
     5281 ttccaaacga tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca
     5341 ttggcttcag ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg
     5401 tatccgtctt ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa
     5461 ataacagtgg ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga
     5521 caacctgaga caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg
     5581 acattcacgt taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt
     5641 cctgaaaata ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat
     5701 accggcattc aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc
     5761 attcatctta cttacaacta tcaattaaat actgtagata cttctgtaca atccgtcagc
     5821 atactgacaa aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt
     5881 cctaacccag ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca
     5941 ttagttaatg taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca
     6001 agttattatg aagcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc
     6061 acgtctcctt ggcaactgac agggacattg acttctttat ttaagaatga tcaaggtcaa
     6121 gaattaagcg gaacaaaatt atacttcaat cattctggat caaaacaatt gattcagcaa
     6181 ggtcaaaaca cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac
     6241 tttcccgata ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc
     6301 gcaacgtatc aaggcatggt cacatgggaa ctgacagctg ggccaaccag ctaagtgatt
     6361 agaatttgta tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa
     6421 acttctgctg ttaaaatcag aaatgcttgg aggagtggac ttggaacttt tcagtttgct
     6481 tgaaaaaaat gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga
     6541 gatttccgtt aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga
     6601 tctgttttat ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga
     6661 agggaatatt ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg
     6721 ctacttgcgc tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt
     6781 tgatcggcag aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt
     6841 gaaggaatta aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac
     6901 cggtccagaa agtcaaattt gttattttta tttccaatta ttttggtttg gacgtcctta
     6961 tcttgaaaac aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca
     7021 aaaagagttt agttttacta ttacagaaga aggacgattg aaatttgaaa tatggctttc
     7081 cattatgaag aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc
     7141 tgttggattg tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta
     7201 cttctttcaa tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat
     7261 gatcagtaac tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga
     7321 aaaatacaaa gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga
     7381 atatttctct ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat
     7441 gcaaaatcac gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg
     7501 attaaaacag cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt
     7561 ggtcagacaa acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt
     7621 tgaattattt gctcgctatg catctatttt gcggacgctg atcaagcagt tagaattacg
     7681 tctgcaaatt gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat
     7741 catccaggaa caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa
     7801 gcagtatgat atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa
     7861 agtttttgta ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt
     7921 aaaagaatgt tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc
     7981 attggtgctt cccatgcagg catcacagct gccctaaatc taagaaaact tcaaccgaaa
     8041 gcagaagttt tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga
     8101 atcaaccttt atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg
     8161 actcttcaag aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca
     8221 gacaaacatc agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat
     8281 aaattaatcg tagcaacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa
     8341 aatgtctaca cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa
     8401 gcaaaagaag tagtgatctt cggtgctggt tatattggat tggaattagc agatgctttg
     8461 agaaataaag gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat
     8521 ttcgataagg atatgatcaa cagttttcaa aatcagctgc aaactaagca aatcaatttt
     8581 tatccaaatg aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa
     8641 cttctatctc aagcaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat
     8701 acgacattgt taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa
     8761 tatctgcaga caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt
     8821 gacaaaaata aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga
     8881 gcagtagcgc taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca
     8941 gcagcagtca ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca
     9001 ttcttggaac aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgat
     9061 gaagaaaaca aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt
     9121 ggcggacagc tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc
     9181 gtgaaacaca aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa
     9241 cacacctctc gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca
     9301 aatagcaata gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga
     9361 tataataaat attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt
     9421 ttttgagcat aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt
     9481 atttcaggag gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac
     9541 gttatgcgtt agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg
     9601 ggttagctta tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt
     9661 cgattgtgct ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga
     9721 tagcagaaga agtcgaaggt gggaaagtgg cgtatcttgc ccagacacct ggagtcgtga
     9781 ctagtaaaga cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa
     9841 atcaattcat catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag
     9901 taggcgatat tagtttgaaa gaagaatatg gtctagccgt aggagataca gttaagttat
     9961 ctggtagtga caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct
    10021 caccagttct ctatacaacc atcaatgctt accaagagat ccgcttcgaa aaagaagata
    10081 caagtgaaaa tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg
    10141 aagatttaga acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc
    10201 aagtattgac ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg
    10261 ggattttcat ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac
    10321 aagggatcac aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat
    10381 ttgttggtat tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg
    10441 cggttccatt ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgctggtag
    10501 tagctgtgtt aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg
    10561 caattggata agaaggagga agagcatgaa agcaatagaa tttaaagaag tagaaaaaag
    10621 tttccaagat ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa
    10681 aggggaattt gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt
    10741 ggcaggcgga ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag
    10801 taatcaaaaa gaaagtgaac gcgttaaact acgttttaaa gaaattggct ttattttgca
    10861 agcttccaat ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat
    10921 caaacatgaa catcgtcaaa aagaagcaaa agaattattt gagcagcttg gcgtggataa
    10981 gttgatcaac aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc
    11041 tcgtgcattg tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga
    11101 ttcagaaaag gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa
    11161 agcaacgatc atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat
    11221 gatggaagat ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc
    11281 atcgatacaa caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt
    11341 ccaagggata tctcggcact tcaacaagag agatcgcaca aaaagttggg atcacccaac
    11401 ccaatctcta tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc
    11461 acttaaaaga tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc
    11521 aagtaacctt gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc
    11581 tgatgcttca cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc
    11641 tatggaagaa aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc
    11701 taagaaatgg gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt
    11761 acatcactcg gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt
    11821 tatatttgaa tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaaatagtta
    11881 ggtgaagaag caagatatga cgaagatcgt tatatcttgt tttttttata taggtagttt
    11941 ttcataaata caaatttgtt attctctcat gattatcagg gtatattttt atttattatg
    12001 tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa
    12061 ggaaactatt tattgggtag atgaatcacc acgaatagtc aaaaggagat ttcaggatat
    12121 gccaaaaatg aaaaaaatca ttttagcagt aatgttattt tctacggtta tatcttttac
    12181 agcccctttt ttgcgtactt aagctgattt taagttttga cccttagtct ggatctacga
    12241 ggtgagagtc aagaatgaaa aaaataattg gagcttttgt atttgcaatc attatcatta
    12301 ttatctgttt tcaaatagat gtttttggtc aaaaaaagat agttggacaa acggtcagca
    12361 gtacgaacag tacggttaca aagacaaagg aatcatttga tgatacagaa caagagacat
    12421 taatcaaaat cggaaaaaat gaccgtacac tatatacaga tgactcgcta actaccccac
    12481 ttgcaactat aaacggcgga gagctggcgg agtttctttc tgaaacaaaa aatgcttatc
    12541 agatcaagac aaatgatggc tatactggat atttagccct cgaagatgga accaaagtca
    12601 caaagaatat acaaacaaaa ccggaagagc tatctgatgc agtgattgta ttagatcctg
    12661 ggcatggcgg aaatgatact ggggcattaa gcaatgatga acaaacagaa gaaaaagata
    12721 taacattaag tacagccata aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt
    12781 atttgaccca tactacagat gaattagtac agcttggaga tatatgcgac tacagtgaag
    12841 aaaaaaaggc agatgtcttt atcagtttac acgcagactc tacggaatat gcgaatgaag
    12901 caactggaat cacgacatat tattactatg gacaagagga aacattagca cagacaattg
    12961 ctgactcctt cactgatctg cctctggatt caagaggaat ctccactggg aactatcaag
    13021 ttcttcgaga aaatcttcaa ccttctattt taatcgagat gggttatatg aataatgata
    13081 gtgatttaaa ggaattagta acagacagtt atcagcagca gattgctgca agtttgacac
    13141 aagcattgac cacttatttt cagtaataaa taagcccagc caaacaaaaa tagctcctca
    13201 tatttttcgt ttggccgggc ttattatcgc agatgcaagc ttttgaacac cgtttaagga
    13261 agaggttgtg agcctgccgt ttaactccgc gtcacaagga gcaatttcaa aaaacagctt
    13321 ctcatgtttg atgactattg tgacttgtga ccgaggagtt ggcatgagaa caccgtttat
    13381 tcggaacaag ggactgtgac atgacttttg tcacagttcc attatttttt tagcagcttc
    13441 aaagcttttt ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg
    13501 cctttctcca atagttttgc cgctacttgg tcagatttcg ttccagctcc tgatggaagg
    13561 gtgattccag cttcagctga tgctttgtgt aactcttcca aaaagctggc acgactgatg
    13621 atagaagcag cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt
    13681 tcttgtaatt gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac
    13741 tgatcgatca aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc
    13801 gcttgattgt gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg
    13861 tatttttctg gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca
    13921 agctggatga tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat
    13981 tctgcaagct gatcacgttt cacataagca gcacatactg ttagcggacc aaaatagctg
    14041 ccatttccga cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa
    14101 gaggctgttt ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct
    14161 attgctgcat tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc
    14221 ccgtcttttt ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga
    14281 tagtatgctt tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata
    14341 aaattctcct cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa
    14401 gagattgcga gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa
    14461 atatttgtgg agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca
    14521 aataggagga aatgattcgt atggcgcacg aaaaaacacg ctacaaagca gtgattgcca
    14581 accaaacata tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc
    14641 tgatcaatga acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag
    14701 cagcaatctt gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct
    14761 tggagctgga agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg
    14821 aaaatcgtgt gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa
    14881 atggtcaagc agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga
    14941 aacgaaaagg acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa
    15001 aaatttaaaa aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt
    15061 tattgctaat tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg
    15121 cgattggata tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc
    15181 atttggaatt atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg
    15241 atcaggtatt tgcttttcac ctagatgaag ctttttatgc aggaactgct tttctgatta
    15301 ttttgctaat tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt
    15361 atgtgccgat cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg
    15421 cttacgttac tatatttttg attttgcagt tacttgcctt tgttccatta gatgtggtcc
    15481 aaaatcaatt cggcgagcat agtttcgctc gttttatcgt tgagagaacg ccatttctta
    15541 ccaacaagat gtatgagctt tgggtaacga atattttgaa ttaaggaaaa tagtgctgga
    15601 aaatatttcg ttttttgtac agagtagttt atttaagaag gctgcgtagc gtatgtccgg
    15661 cacagctctt ttttcatagt gaaagataag gtgataaaat gaataaccga atccttgaaa
    15721 cattggaatt tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact gcacaaggaa
    15781 aagaagaact ggcagaattg gtaccagtga gtgagaaaca aacgatcaca aactggcttc
    15841 aagaaactga ggatggattg aaagtgcaac gtctgcgagg cgggatccct atacctaaac
    15901 ttgaaaatat ccgtccccat atgaaacgga tcgagatcgg agcagacttg aatggagtgg
    15961 agcttgcaca agtttccaga gtattgtcta cgacaagcga attgaatcgt ttcattgatg
    16021 atctttctga cagtgaaatc gaatttgcac gtttgtatat gtgggcagat cagttagtca
    16081 cgatccctgt tttgagccgc cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg
    16141 atgacgcttc tcctgagtta aagagcataa gacagaacat tcggcgaagt gaacaagctg
    16201 ttcgtgaaca gttagatggg atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga
    16261 tcatcaccat gcgaaatgac cgctacgtta tcccagtcaa acaagaatac cgcggtgtat
    16321 ttggcggtgt agtccacgat caaagtgctt caggacagac tttatttatt gaaccaaaac
    16381 aagtggtgga tttgaataat cgtttgcgtc aataccaaat cgctgaacga aatgagattc
    16441 aacgtatttt aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa atcatccaca
    16501 atgcttatgt gattggtaaa atggatctga tgaatgcaaa agcacgattt ggtaaagaag
    16561 taaaagccat cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa caagcaagac
    16621 atccactgat cgatcaggaa aaagtagttt ctaatgacat cacaatcgga aaagactatc
    16681 aagccatcgt cattacagga cctaacacag gtgggaaaac gatcacgttg aagactttag
    16741 gtctgttaca actgatgggg caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa
    16801 tcggtatttt tgaggaagta tttgcggata taggagacga acagtcgatt gagcaaagtc
    16861 tatcgacctt ttcttctcat atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa
    16921 aaagtctggt attatttgac gagctcggtg caggaacaga tccgcaagaa ggtgctgctc
    16981 tagcaatcgc aatcttagat gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc
    17041 attatcctga attgaaagtt tatggataca atcgggcaaa tacgattaat gccagcatgg
    17101 agttcgatgt ggatacacta agtccaacgt atcgtttgct gattggcgtt cctggacgaa
    17161 gcaatgcgtt tgaaatctct agccgattag gtcttgatac agaagttatt gatgaagcga
    17221 agcagttgat gaatgatgag agccaagatt taaacgaaat gatcacagat ttagagaatc
    17281 gccggaaaat ggcagagacc gaatatctgg aaatgcgtca ttttgtttct gaggcacaag
    17341 aacttcacga tgatttgaaa gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa
    17401 tggaaaaagc aaagaaaaaa gcaaacgaag tcgtgtcaga ggcggaagaa aaagcagaaa
    17461 aaatcattgc tgatattcgt aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc
    17521 atcagttgat cgatgcaaag acccaattag caaatctaca tcaagaagaa actttgaaga
    17581 aaaataaagt attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt
    17641 tggttacgac ttacggccaa agagggacgc ttttacgtaa aaacgggaat cagtggcaag
    17701 tagaaatcgg tatattaaaa atgaatgtgt ccgaagacga gctgacgcct gttgctcctc
    17761 aaaaagagcc gacacaacga gttatccatg ctgtacgatc tgaatctagt agtcacgtgc
    17821 cgaatcaatt ggaccttcga ggaaaacgat acgaagaagc gctaagtgag gtagatcaat
    17881 atttggattc tgccattcta gctggttatc ctcaagtgac tattgtccac ggaaaaggaa
    17941 caggcgcact tcgcaaggga attactgatt atcttaaaaa ccatcggagc gtcaaaagct
    18001 ttgagtttgc accggcaaac caaggcggaa atggtgcgac aattgttaaa tttaagtaaa
    18061 gcttactaaa ggtaagcaga tggttcgcta tccaagtata gaaatgctat actaatcata
    18121 gattttaaaa atggaggtct ttgatatgtc acaagtaatt acagataaga catttaatga
    18181 agaaacagat aaaggacttg tattgattga tttttgggca acttggtgtg gtccatgtcg
    18241 tatgcaagca ccgattttgg atcagttgga acaagaatat gatgaagaag aattcagaat
    18301 tgctaaaatg gatgtggatg aaaatccaga aactcctcaa caattcggta tcatgagcat
    18361 tccaacatta atgttgaaaa aagatggtca agtagtagaa aaagctgttg gtgttcattc
    18421 aaaagaacaa ctacgtcaaa tgatcgatca atatttataa aaacttacag caaaaataag
    18481 ctgagggtgg gactgaatcc agttccgtcc tcggtttttt tgcggttgag ttaaggttat
    18541 tcaaattaat attttttgag tataattacg ttgtcaggct ggattgtttt tttgacagac
    18601 atgctataat aactttaatt gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa
    18661 acaaattagc tttacttcct gatcagcccg gctgttactt gatgaaagat aaaaatggaa
    18721 cgatcattta tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca tattttactg
    18781 gcagccataa tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt gaatacatcg
    18841 ttacagaatc gaatatcgaa gcattgctct tagagatcaa tctgataaag aaaaatgatc
    18901 ctaaatacaa cattatgtta aaggatgata aaacctatcc tttccttaag ataacgaatg
    18961 aaaaatatcc ccgtttggtg attactcgta aagtactaaa agacaaagca ctgtattttg
    19021 gaccatatcc cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat cgattatttc
    19081 ctctgagaaa atgcaaacct agtcagacga aggagccttg tctttactat catttgggac
    19141 aatgtctgtg tccttattat ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg
    19201 aagtgaaaaa gtttttaaac ggaggacacg aaaaaatcga gtcagagatc catcagaaaa
    19261 tggaaaaagc tgctgaaaac atggagtttg aaaaagctgc agaataccga gaccagatcc
    19321 gtgccattga gacaatcatg actcgccaaa aaatgacaaa tactgattta ctggatcgag
    19381 atgtatttgg ttatgcgatt gacaaaggat ggatgtgtgt ccaagtgttt ttcgtaagac
    19441 aaggaaagct aattgaacga gatgtttcaa tgtttccttt ctataacgaa gcagaagaag
    19501 atttcttgac ttacctcggt cagttttatc aggaaaatga gcatttcatt cctaaagaag
    19561 tcttgattcc tgatacgatc gacaagccca gtgtagaagc tttgttagct acgaaagtgc
    19621 tgcaaccaaa acgaggagaa aagaaaaaat tagttgaatt ggcgaataag aatgcctcag
    19681 ttgctttgaa tgaacggttt gatctgattg ccagaaaaca ggaacggacg atcggagcag
    19741 ttgaacgttt aggaaacgcg atgaatatcc cgaccccaat acgtatcgag tcttttgata
    19801 actctaatat catggggacg aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac
    19861 cggcaagaaa agaatatcgg aaatataaaa taaaaacagt caacggaccg gatgattatg
    19921 catcaatgag agaagtcatc tatcgcagat attcacgagt gattcgcgaa gggttgcctt
    19981 tacctgattt gatcttgatc gatggaggaa aaggacaagt agatgtggca aaagatgtct
    20041 tagaaaatca attaggaatc gatattccta ttgctggcat ggcaaaaaac gataaacata
    20101 aaacaaatga gttgctattc ggtatagatt tggctgtagt accgcttgag cgaaattcac
    20161 cagagttttt cttactccag cgaatccaag atgaagtcca tcgattcgca atcactttcc
    20221 atcggcagct acgcagtaaa aacagttttg cttcccgatt agatgaaatt gaagggttag
    20281 gaccaaaacg aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat atcacagcag
    20341 ccacgacaga agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg
    20401 ataaattaca tcaggaatga ggaaagggca ctttacaaag aaagtaaagt gtcctatcat
    20461 tttatgtaca acaaaaagag cgtttacgtt ttgttggtaa ataataaatc gcttacgtct
    20521 ggtgtctaag gatatatgtg atttcttgaa gaaggagtga agaaatgtta gaagtcaagg
    20581 atcttgttaa aacttttggg aactacacag ctgtcgatca tgtctctttc caaatccccg
    20641 atggaaaaat catggggcta attggccaaa acggggctgg gaagacaacg acttttcgtt
    20701 tgatcttgga ttttttgaca caagaccaag gagaagtgtt atggaatggc catcatcttg
    20761 gggagaaaga ttatgatgtc atcggttatc ttccggagga acggggactt tatccgaaag
    20821 taacgattca agaccaactg ctttattttg cagaacttag agggaaatca agaaaagaga
    20881 ttgaaccaaa aatcgatttt tggatggaaa aatttcaggt aaaagggaaa aagacggata
    20941 aagtaaaatc tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc
    21001 atgaacctaa attgatcatt ttggatgagc cattcagcgg gttagatccg gtaaatgctg
    21061 aattgctaaa agatggaata ttggaattga aaaaacatgg ttcatgtgtc attttttcta
    21121 gccacaatat ggataatgta gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa
    21181 aaatggtttt gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt acgaagttat
    21241 tcttggaatc tggtttaagc caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga
    21301 tccttcatga agacaatacg ttagagatta ccttagaaga tcctgaagct gggaaagaaa
    21361 tctttactcg tgcgactcag ttcggctaca ttcctatgtt taaccagcaa ccgccaactt
    21421 tggaagagat tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg gattattacc
    21481 aaagatgtct atttaaaaaa tgttaagtcg atttctttct tgatcatgat attagtccct
    21541 tttatcatgc tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc
    21601 aataaaatcg gcgtattggc tgataatgag caaatcagtc agcaaatgag ccaagtcaaa
    21661 acagatgatt ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa agagctttcg
    21721 gaagaaaaaa tcgatgcgta tttagtagtc tcaactgaga atgataagat aaatgggatg
    21781 ctttatagcg aaaattcatt aggacaaagt acacaactac tgatacaaca acaactgact
    21841 ggcttgcaat cgatgcttcg agcaagtcaa ttaggcattt caccagaaga agtagctagc
    21901 ttgagccaac aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg
    21961 acagttggag aagataactc gacagtccaa tatgtcgtaa gttatgtagc aacgattgtc
    22021 ttgttcatta ttattttgac gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa
    22081 ggcacgcgga tcatggaagt gatcctgtcc agtacgactg cacaaaagca tttttacgga
    22141 aaactaacag gtgttttact tgttgcatta acgcaaatgg cattatacgg tattattttt
    22201 ggtataggat acagtcaatt caaagatatg gaaatggtca aaaatatatt agacggtatc
    22261 tcattggata acttgattgg accattcttg tggtattctc ttctattcat gatctttggc
    22321 attttgattt tttctgtttt agcagcttta tgtggctctt tggtcaacaa agcagaagat
    22381 acagcaaaag caattttgcc agtgacttat ctatctctag gcggatatat gcttggattg
    22441 atactaggag cttcagatcc aaacaatatc gttattcgga tcacttcgta tattccattc
    22501 ttatcttcat atattatgcc ggttcgctta gcaaatgaga cagtcggtat gggtggagca
    22561 ttgatttcct tgttggtact tgtagccgta acattcttat tgatgatctt gtctgcaaac
    22621 atgtacaaat cgaatgtact cgtgtatagc gaaggcggat tatggagttc attgaaacaa
    22681 tcgatttcaa ttatgagaaa tgaacgaaaa aaagcataaa atatagaaaa atatagaagg
    22741 aagctggaca tttattgttc ccagcttctt ttttgtatag acctatttct atttttttat
    22801 tatattggat atatatattc attgaaaata gagcataaca actttataat gttgagtata
    22861 ccaatttttg gaggcgtttt aatggaccgc tatttagatt tactttcaga gaagttccct
    22921 acaacggaag atgtcatcac ggaaatcatc aatctcgaag caattttgca gcttccaaaa
    22981 ggaacagagt tatttttgag cgatatacat ggcgaatttc ctgcttttga tcatattctg
    23041 cgtatcggtt caggaaatct taaagaaaag atcaaagacc ttttctctga acaaatgacg
    23101 gaaacggaga tcagccaatt cactattttt acggcttatc cagaatatgt gttgacgact
    23161 gattggtaca aaaaacaaga taaatcccag ctgatccatc gtttgatcga tcttttacgg
    23221 tttacgacag tcaaatacac tcgatcaaaa gttcgaaaag gattgccgaa agaatatagc
    23281 tatatcattg aggaactctt gtatatcgat gatcgtatca acgggaaaaa agactatgta
    23341 aaaaaaatca ttgaacaact tgtcttgatg aaggaagaag aaaaattttt aacaaaactt
    23401 gcgcagacga tccaaaaatc tgtcatcgat catttgcata tcgtgggtga tatttttgat
    23461 cgaggaactc aagcagccaa agtaatggat cgaatcatgg atttttcctc tgtagatatc
    23521 cagtggggga atcatgatat tttgtggatt ggtgcgtatt gcggctctga ggcatgttta
    23581 ttgacgcttt tacggattgc cgcacgctac aactatttat ttgaattaga gaaagaatat
    23641 ggattaaatt tacgtccatt attttctttt gcccatcaaa cctatcagaa gaatccagta
    23701 tttaccccta aaaacaggaa agatctatct gaaagagagc aagaagaatt agaaaagatc
    23761 catcaagcac tttctatcct ccaattcaaa tcagaacatc agttgttgaa tcgacgacct
    23821 gaatttaaaa tggacgatcg taaattgttg gaaaaaatca actatgagga atcaacgatc
    23881 gttttagaag gtcagctcta tgagttgaaa gggacttgtt ttcaaacgat ccagccagat
    23941 gatcctaaaa aattcttgga agaggaacaa aaagtgatcg atagtttgat gtattcattc
    24001 cagcactcgt taagaatgca aaaacacatg acgtttttga tcgaaaaagg gggaatgtat
    24061 ctcacgaaca atcagcatgt actgttccat ggctgtatac ctgtagatga aaaggggcag
    24121 ctcttagcat ttgaattaga ccaatcttat cgcggaaaag aactgctggg attttttgaa
    24181 aaacagataa ctgaaagcgc aaaagatttg actgcaaatg aagatttggc gaccgatttg
    24241 atttggtatg cgtggagcgg gccattttcg cctttattcg gcaagagtaa aatggcaact
    24301 tttgagcgct attttctcac agagcctcat acgcacgtcg aaaaaagcaa tccctattat
    24361 cacttgcgag atgaagagtg gttcagtgaa aaaattttgg cagaattcga tgcaaaagaa
    24421 gaaggttcag caatcatcaa tggacacacg ccagtaaaag taaaaaaagg agagtcaccg
    24481 atcaaagcaa acggaaaagc atttgttatt gacggaggtc tatccaaagc ttatcaaaaa
    24541 acaaccggga ttgctggcta ctcattacta tgcaattctt acggtttcca aatcgtgaca
    24601 cattatccgt tcttgcctat tgaacaacaa tttgcaaaaa aatctgatca aacgaatgta
    24661 aaaaaagtga tcgagcagca gctcccacgc aaacttaatc ggaatacgac aaaaggaatg
    24721 gaattacagc aacagattac tcaactccaa aggctgttgg attatcgaaa ggacgattga
    24781 gaaaaatgct agccaacatt gctcaataga ctaaaaagcg actgcaacaa aaggcgtgtc
    24841 gcagtctcct tcggaattag ctcaaaaaac ggtaaaatat tagaagctat ttttctgttt
    24901 atttctctaa tactcaggtc agggcgctga tttcacgacc tcttccttaa acggtgttct
    24961 cctgccaact cctcggtcac aagtcacaat atccaacaaa catgagaagc tgttttttga
    25021 aattgctcct tgtgactcag agttgaacgg caggctcacg acctaatcag aaaaacgatt
    25081 aaaattcatt cacaaagaaa tcgaataaac gctgttcaga tgaatctaca cgatgagaaa
    25141 gttccggatg ccattggaca ccaaggattt tgtgcatatt atctttactt tggactgctt
    25201 caatgagtcc atcgttggaa aaggcgatgg cttttaacaa aggagaaaga tctttgactg
    25261 cctgatgatg ataggaattc acaaagtatg aatcagggag aaggtcactt aataaactgt
    25321 ccgaaacgat tttgacttcg tgagttgcaa actgcggggc agtgggctgt tgttcgtgtt
    25381 ttacggacca ttttggatat aatgaaagat cttgataaag tgttccctcc aacacgacat
    25441 tcaatagttg aagtcctcgg cagactgcaa aaatcggttt tctttgtttc aatgcttcca
    25501 agatcaatgc ctgttcgaat tggtctcgtt gaatatttgt ttccgtcagt tttggatgag
    25561 ggtcttccat gtaaagttgc ggactaacat cctgaccgcc tgcaagaatg agtttgtcga
    25621 ttttctcaat tagttgaggc gctgaatctg gagacatgac tggcagaata agaggtaatc
    25681 cttgagcatc taaaactgca tccacaaatc cctgcggtgt atagcttact tgatttcctt
    25741 ggaaagtatc tgtcgctcga atcagttgat tcccggcaat gccgatgatt ggtttcattt
    25801 ttattccccc taaagtagac taattgtaac aaggatttaa aaaaatttaa actatgtaag
    25861 acttgcccct atacttatct gttcttgctg tgttactatc tgtaaagaga attttcgtgt
    25921 gaaattcatg acgatagttg aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa
    25981 ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc aaggggtgag aaggacattt
    26041 tattgaaggg gcctgcctat actaagttat ctttagaggt acatactttc aatgaaaaaa
    26101 gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag caagtgttag aagctttgga
    26161 acaaatccga aaagcagaag aagaaaatga gaagcgtgct cgaaccctac ggcagcagtt
    26221 gaagacctat gcagaagaaa aagagcaaga attggagata atccaaacga acagtcggca
    26281 acagctgaaa cagttggtag aagaaaaaga acaattcgaa aagcaagaat tagaaaaaga
    26341 aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca actttgaaag accaatatca
    26401 aaagcatcag agtcaagcaa tcggtgcaat cattgaaagg gtgaaagaaa cttatggccg
    26461 tcactaagat ggaaaaagtg accctcattt cagataaaag aaatcaggag aggattttac
    26521 aagcagtaca aggaatccaa aatgttgaaa tccgggatct gtatcaaaat agaacaaaca
    26581 accagtgggt agaaaaatat tttccagata ccacgattat tgataaagaa gcaaaattaa
    26641 gcgctttagg caatcggctg aaagacatcc aagaagctat ccaattcata caacaccacg
    26701 gcgatagccg tcaaaagaaa ctccatttga aacatcaaga actttcctta caagacttgg
    26761 aagcaaatta ttccgaagaa aatttcatga ataaattaga agaagtcctt gatttaaaag
    26821 aaaagtggga acagctaact gaaaaacgtg atcgtttaga agaagaggaa gaatggcttc
    26881 ttcattggca acagctggat attgctccaa gtatgtatgc aagtaagacg accatttttg
    26941 agatggggac tgtgagtgca gcaaattttg aaagtttcaa ggaagaacta agtaagcttg
    27001 atgacgttta tatggaagaa atagattata cgccaaaaca agtttatctt tcgtatgttg
    27061 ctttaaaaaa atcagttgct gctatagaag aaattgctgg gaaatacggg ttcaataaag
    27121 aaacgtatcc ttatgaacaa aatcctaaag aaaaattgca agaaaccaaa cagcaattat
    27181 ccattgttca cgagcagcaa aaaaagataa cgacagcgtt aggtcagtgc agcggctata
    27241 tccaagaatt tgaatgggta gaagaagtca cacttgccgt tgcagaacgg gaaaaagtaa
    27301 aagaacggtt tatccatgcc acttatctga tcgttttgca aggttgggta gatgcagaag
    27361 aaaaacagaa tttgatgcat gtgttaaacg aaactgtatc agaagaagat atatacgtta
    27421 gtttcgaaac accaaactca gaagagattc agcaggaagt ccctactaaa ttgaaaaacc
    27481 atccgcttat cgaacctttt gaattgctga ctgaaatgta cagtctgcca aaatatgaag
    27541 aagtagatcc aacgccttgg atgacaccat tttatcttgt cttcttcggg atgatggtcg
    27601 cagatgttgg ttacggattg ctgatgctca ttgggacaat actagctcaa aaactatttg
    27661 ttttaccccg cgggatgaaa cgtttcgtaa aattctttga gattctctcg gtaccatcga
    27721 tcatctgggg attgatctac agttcatttt tcggacaggc attaccaaaa gaactatttg
    27781 gtatccgctt gcctttccca atcctatcta cgacggatga cgtcaatacg attttgattt
    27841 tatccgtgat tttcggtctg atacagatct tggttggatt attcgtggca gcaaaggaaa
    27901 atatcaggag gaaagattac ttgagtgctg tcagtgatgg ttttgcctgg caagggatcc
    27961 tgattgggat cgttattgca ttgattggtg tcatgttgat aaaaaataca gcatttgttt
    28021 atcttggcgc ggggctatcg atcattgctg ctttatgtat cttaatcgtg ccgattatcc
    28081 aatcgccgtc taaagtcaag ggtgctgcca aaggagcgta taatctttac ggattgactg
    28141 gttatatcgg tgatttagtc agttatacac gtttgatggc actaggtatt tcaggcggaa
    28201 gtattggcgc ggcattcaat atgctagttg ctttcatgcc gccagccgct cgattcagtg
    28261 tagggatcct tttgatcatc gctttacacg cactgaatat gtttttgaca ctgttaagtg
    28321 cctatgtcca tggtgcacga ttgcagtatg tcgaattttt tggaaaattt tatacaggag
    28381 gcggacgtgc ctttgacccg ctgaaaacgg cggaaaaata cgtcaacatc aatcataaga
    28441 aaaaaaacaa taaataattg gaggattttt atacaatgat ggattactta attagtcaaa
    28501 atggtggaat ggtatttgca gtattagcga tggcaacagc gacgattttt tcagggattg
    28561 gttcagctaa aggtgttgga atgacaggtg aggcagcagc agcgttaaca acaagtcaac
    28621 ccgaaaaatt cggacaggct ttgattctgc aattgcttcc aggtacgcaa gggttgtatg
    28681 gattcgttat cgcctttttg atttttatca acttaggaaa tgatatgtca gttgtacaag
    28741 gattgaactt tctaggcgct tctctgccga ttgcttttac aggtttgttt tcaggtatcg
    28801 cccaagggaa agttgcagca gcaggtatcc aaattttagc gaaaaaacct gaacatgcta
    28861 caaaaggaat catttttgct gcgatggttg aaacatatgc gattttaggt ttcgttattt
    28921 ccttcctatt agtattgaat gcctaagcat aaaggaggaa aagcagatga acgccattga
    28981 aaaaatcatc tctcagatga atgaagcagc agagcaagaa agagcggcgt tagagcaaga
    29041 agagcgaatg aagatcgatc aaaactttga acaaaaacgt acgcaaatcg aaacagaaca
    29101 tcaaaaacaa aaagaaaaac aaatcgaatt acttgaaaaa aaataccgtc agcttaggaa
    29161 tcgccaacaa gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat ttttacgacg
    29221 attgtttgca gatgcaatag ctgaaatgga gaattgggat gaatctgaac aaatccagtt
    29281 tatcaaaaat tcattgtatt cattaccttt gacaggcaaa gtaacattca ttgctggaga
    29341 aaaatcagca gcttatcttt ctcagacatt gctggatgaa tggaacaatg aacttccctt
    29401 tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg ggttttctga taaacgatca
    29461 aggagttcag tataattttc ttttcagcag ccttgtacaa gatatccaag gaacgatgag
    29521 ttttgagatt gccaatcaat tattcgaata aggagggatc agtgtatgga ataccacgaa
    29581 ctaaatccct tgattcgcgg aagagagctt gaacttttgt ccaatgagac atttgaacgg
    29641 atgattcaaa caaattctgt cgaagcactt ggtgaaatat tgaaatccac gatttacagt
    29701 ccatatattt atgacggata tgaatatgac ttcgaacaga atcttttaaa ggagcagagc
    29761 cgtctatttc gctggttaaa ggaatcagca ccggagcctg aaatcgtttg gatctatacg
    29821 atgcgctata ctttccataa tctgaaggtt ttgacaaaag ccgagatgac aaatcaaaat
    29881 ctggaccatt tgtatatcga cgatggattt tattcattag aaaccttgaa ggatgccatt
    29941 catacgcaag tttcaaatga actccctgaa agcattatgg acagtatacg agaagtacgt
    30001 gattattttg aagagtctac cattcttcag ggaattgacg tgatttatga tcgacagttt
    30061 ttgacagagc agcgacggct tgccgataag ctaggatatc aagaattgct gacagaagtc
    30121 attgctttta ttgacttgac caatatcaca acgatggctc gaggaatatt gcagaaaaga
    30181 acatatgggt tcatgacgac ggtactttcc agctcgggaa gtatcgaaaa agaagtattt
    30241 ttgaactttg tagataaaga tatggaagat tacacgaatt ttctattaga gacagattat
    30301 gctgattttc tgcgccctgt catccgtgag ggaaatcttg atctcgtaaa gcttgaacag
    30361 ttgaaagatg attatctaag ttcattgtat caagaagcac aaacccaggc tttcggacca
    30421 ctgccactac tagccttttt gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg
    30481 gttatcggca aaagaagcca tttcgaacca gaaacaatca gaaagcgggt gagacaagtc
    30541 tatgacgcat aagatcggtg tagttggcga caaagattct gttttgccat tcaaattgtt
    30601 tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat
    30661 ggcaaagaat gagtatggcg tgatttatgt cacagagcaa tgtgcagcat tgagcccaga
    30721 aacgatcgaa cgttacaagg aacaaatgat acctgctatc gtattgattc ccaatcatca
    30781 aggatcatta gggatagggc tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca
    30841 aaatatctta tagtatgaaa aggagataga aattttgcaa attggaaaaa tcataaaagt
    30901 ctccggacct cttgtaatgg ctgagaacat gtctgatgcc agtatccaag acatgtgtct
    30961 agtgggagat ctaggagtta tcggagaaat tatcgagatg cgtggcgatg tggcgtctat
    31021 tcaagtatat gaagagactt ctggaattgg tccaggcgaa ccggttcgct ctactggtga
    31081 agctttatct gttgaattag gaccagggat catttcacaa atgtttgacg gaatccaacg
    31141 gccattagat acctttatgg aaatcacaca aagtaatttc ttagggcgcg gagttcaatt
    31201 gcccgcattg gaccatgaga aaaaatggtg gttcgaacca acagtagaag ccggagaaac
    31261 ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca aaagttataa aacataaaat
    31321 catggtgcca aatgggataa aaggaacgat caaaacaatc gaaaaaggtt cattcactat
    31381 tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac aaaaatttga cgatgcttca
    31441 aaaatggcca gtaagacgta gtcggccaat caaagaaaaa ttaaacccgg atgcacccat
    31501 gatcactgga cagcgagtga ttgatacatt cttcccagtg acaaaaggag gagcagcagc
    31561 tgtacctggt ccttttggtg cagggaaaac agtcgttcag catcaaattg caaaatgggc
    31621 cgatgtggac atggttgtct atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt
    31681 tttaaatgaa ttccctgaat tgattgaccc taatacagga gaatcgttga tggaacgtac
    31741 ggtattgatt gctaatacat caaacatgcc agtagcagct cgagaagctt ctatctatac
    31801 gggaatcaca attgcggaat atttccgtga catgggttat gacgttgcta tcatggcaga
    31861 ctccacttca cgatgggcag aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc
    31921 tggtgatgaa ggttatccag cttatcttgg ttcacggctt gcggaatact atgaacgatc
    31981 tggaagagtc attgcattag gatcagaagg tcgcgaagga agtataaccg caatcagtgc
    32041 tgtatccccg tctggagggg atatttcgga accagtcaca caaaatacat tgcgggtcgt
    32101 aaaagtattc tggggtcttg attccagttt agcccaaaaa agacattttc cttcgatcga
    32161 ttggatacaa agctattctc tttattcaac agaagtaggg aagtatatgg atcatatcct
    32221 tcaagctgac tgggcatcca tggtcaccga gggcatgcgt attttgcagg aagaacagca
    32281 gctgaatgaa atcgtgcgtt tggtagggat tgattccctt tcagataatg accgtctgac
    32341 attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt
    32401 cgatacattc acttcaagag agaaacaatt caaaatgttg aagatgatct tgacctttgg
    32461 tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta agtgagatca tggacggtac
    32521 cgtatcgatt cgtgaaagga tcggacgaag caaatatatc ccggaaacag aattagaaaa
    32581 actagatcaa attattggtg aaatcaagca aacgatgcaa acgatcgttt cagaaggagg
    32641 catgagcaat gattaaagaa tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg
    32701 ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga agtccgtatg caaaatggcg
    32761 agattcgtcg tggacaagta ttggaagtcc aagaagataa agcaatggtc caaatattcg
    32821 aaggaacaag tggtatcaat ttacgtgact cttctgtacg atttttaggc catcctttag
    32881 aactaggtgt atcggaagac atgatcggtc gagtattcga tggtttggga cgaccgaaag
    32941 ataacggacc agagatcctg cctgaaaagc atttggatat taacggagaa gtaatcaatc
    33001 cagtagctcg ggattatcca gatgagttta tccaaacagg tatctctgca atcgatcatt
    33061 tgaatacatt agttcgtggg caaaaattac cagtattttc tggctctggt cttcctcata
    33121 aagaattagc tgctcagatc gcgcgacaag caacggtact cgattcatca gatgatttcg
    33181 ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc tgaatttttc atggaagatt
    33241 ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat gaatttagca aacgaccctg
    33301 caatcgaacg tatcgctact cctcgtatgg ctttgactgc tgcagaatat cttgcttatg
    33361 aaaagggaat gcatgttttg gttatcatga cagacatgac caactatgca gaggcattgc
    33421 gagagatttc tgcagcacgg cgagaagttc caggaagaag aggatatcct gggtatcttt
    33481 acacgaactt agctacttta ttcgaacgag caggaagaat ccgcggacta aaaggatcgg
    33541 ttactcaaat tccgattcta acaatgccag aagacgataa aactcatcca atccctgact
    33601 tgactggtta tattacagaa ggacagatca tcttgactag agagctttac aagagtggga
    33661 ttcagccacc aattgatgta ctcccgtctc tttcgcggtt aaaagataag ggaactggac
    33721 ccggaaagac aagagaagac catgcagcta cgatgaatca gctttttgca gcctatgcac
    33781 aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga atctgcttta tctgatgtag
    33841 acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga atatgtcaat caaggattct
    33901 acacgaaccg ttcgattact gaaacacttg atctagggtg ggaactgcta tcgatgttgc
    33961 caagaaccga attaaaacga atcaaagacg acatgctgga caaatatctg ccagaaggga
    34021 agtgagttct gatggcacga ttgaatgtga atccgacccg catggaattg actcgattga
    34081 aaaaacagtt gacaactgcc acaagagggc ataaattatt aaaagataag caagatgaac
    34141 tgatgcgtca attcatcctg ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag
    34201 agtctatgac agaagcaatg tctgattttg tattggcaaa tgcttcgatc acggaacctt
    34261 tcatcgaaga attatttgtt ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa
    34321 atatcatgag tgtaaaagta cctgtgatga attttgaata tgatgaacag ctgacagaca
    34381 cacctttgga atacgggtat ttgaattcca gtgccgaact ggatcggtca atcgatcggt
    34441 ttactaccct gcttccaaaa cttttggaac tgacagaaat cgaaaaaact tgtcaactga
    34501 tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc attagaatat atgacgatcc
    34561 ctcaattaga agaaacgatc tattatattc gtatgaagct ggaagaaaac gagcgcgcag
    34621 aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac tgactaagtg gtatcttttt
    34681 ttaaaagagg aaaacgatga ttaaacaaag caaacaaata aagaaacgtt tgccggctgt
    34741 tcagcttctc gcattaggat tttttctcct gattttagtt ggcggcggcc tacttaccct
    34801 accatttttt agtaatagtg ggcatgggac aaattttatc gatgcgcttt tcactgcaac
    34861 ttctgctgtc tgtgtaacgg gattgacaac attgaataca gcagagcatt ggaacagtgc
    34921 tggtcaattt ttgatcatgc ttttgattga gatcggcggt cttggtttta tgatggttcc
    34981 gattctatgc tttgcattga taaagaagaa agtcagtctc agcacacgta ttatattaaa
    35041 agaagcgctg aatctagaag aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa
    35101 attggccttt gttatccaat tgattggtac ggttgcttta tcttttgtat ttgtgcctga
    35161 atttggctgg ttgaaaggta tctggtacag tttgttccat gcaatctcta gtttttgtaa
    35221 tgcagggttt gatttgttag gagacagttt agccaatcac cagcaaaatg tttatttgat
    35281 catggtggta tctgctttga tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt
    35341 actggaatat catcagaaaa agaagatgac gattcattca aaaatcgcat taaccgttac
    35401 aggtaccctt ttattaggcg gattcgttgt gttctttttc acagagcgca atggggcaac
    35461 gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc
    35521 tagaacggct ggatattatt cagttgatta cctccatatg agccatgcag gtctgatttt
    35581 aacgatgttt ttgatgtaca tcggcggaac atctgggtcg acggccggcg gattgaaaac
    35641 aactacgtta ggtgtgttgc tgatccagat gcgttcgatg ttgaaaggac gtacgagagc
    35701 ggaagcattt ggccgtacca tccgacaagg cgcagtgttg cgggcactta ctttattttt
    35761 tgttacgttg acgttgtgtg tagtcgccat catgatttta tcggtgactg aaacgatacc
    35821 taaaacttct ggcatcgaat atattgcttt tgaggtcttc tctgcgtttg gtacagtagg
    35881 tcttacgatg ggattgacgc cagacttgac tgcaataggg aaattgatca ttatagcgct
    35941 tatgtacatt ggacgagttg gtgtattgac tgtggtcttc tctctgatga caaaagcaca
    36001 aagaacacaa tcaaaataca aatatccaga agaaagtgtc atgattggat aatttcatct
    36061 tctgacagaa acctagtgat ttagtaaaat aagaaaataa aataattttt cttgcttctt
    36121 ggatacttta agtgcaaaat gttcgtttat tttttgaggc actacccgtt gttgaacctc
    36181 gggtagtgcc tcttttttat tgaaaggaat caataagatt tgattccttt atttatcaat
    36241 aggttataat tcatattttt ttattttttc ttttacgata cttagtccag aagctaaaat
    36301 ttcaaggtta ttagaggata gggaaattcg aacgaatgca tctggttgcg cacgtgtagt
    36361 aaagcgtctg gaatgataaa gtcggatacc gttagcaaga aagtcattct caattatctt
    36421 agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa gggacacgat tcgttggttt
    36481 ttctactttg aaaagagaat caaaaagctg attggctctt tttgtaagac ttaatttagt
    36541 ttctaagatc ctatttgctt catttgaatg gaggatttga gtgatgactt ccacatctaa
    36601 tccagaagtt ttcacattta tattaaaaat tgcttgttct atcttgcttt ggacatgtac
    36661 tggaactaca agataagcaa ctcgcaatcc agaacaaata aattttgtca ttccagctaa
    36721 gtagattgtt tggttaggta ataaattttg gaaaggagga atgatcgtat gttgatagta
    36781 agttgttaaa aatgaatgga tatcatcttc aataacccat agaccttctt tttcaatcac
    36841 ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac cctataggat tattacaaga
    36901 aggcataaga aagacaccat gaatcttctt ttttctacat tcctgaatca atatatcagg
    36961 tcgcattcct tgctcgtcaa aatcaattgg aacgatctct aaatgatata actgagccaa
    37021 ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt ctatcacctg gagagaaaag
    37081 tgcagaaagt gtgatagcca atccattttg aacaccggaa acaatagata gagattctaa
    37141 ggacggaatg actccttgag tttttaacca atttgctgca gtagtcaatt gaaaatttgt
    37201 gcctttggga tcttggtaat taagcaaacc aacaagattt gggttattac tagttgattg
    37261 aataaacggg acaacgaatt tattacattc ttcaaaagaa ctaaccagac caaaatcaat
    37321 gatatgttcg gttacttcat tggtagagat ggtgatggac tcaaagacat tgggagaatt
    37381 aaatgtgcca cttccaatat tcgtatacag caaacctttc tcaattccat atttataagc
    37441 cttgccaacg gtagtaaaat ttaaatctaa ataatcagct agctctcttt gggagggtaa
    37501 ctgagtgttt ttctgtaatt tattctttaa aatatcttgt tctaattgtt gaatcaacga
    37561 tcgataataa ggacgcttca atttttgttt gtctggtcgc caagttaacg ggtaattgtc
    37621 ataagaatta ataggcattt tctccactcc ttaaaattgt ataccataca attttaccat
    37681 tgattgtatt gattttagaa gttaatcttt tgtgaggagg gaattgtcta tgaaaattat
    37741 taattttgag gaagtagtag cttcttttag ttttgaagaa gcgattcaag taatgaaaaa
    37801 atgttttttt gattatgaaa gtggtaaaat cagccaaaat cctcgattgg tggaaatttt
    37861 acctgacgga aaaggcaaaa atatgtttgc gatgatgcct gcttatttgg gggagaacag
    37921 atattttggc acaaaagtca ttaccgcttt tccggataat catcagtatc atttaccttc
    37981 tcatttagga gagattttct tgtttgatgc tcaaaacgga ctacccaaag cattgataaa
    38041 cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct gcgctggcaa cagattactt
    38101 agcaaatccg catgcagaaa cacttgcttt aattggagct ggtcaacaag cattgtcgca
    38161 tttgactgcg atttctacca ttcgtcccat cactacagtg tttgtttacg atattttaga
    38221 agaaagacga aatactttta tcgctaaagc taaaaaacag tatcctaaaa taacgtttat
    38281 aaatgcaata tcagtagaag atgcagtacg tccagcggaa atcatttgta cgttgacctc
    38341 tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa gacgcacata tcaatgctgt
    38401 gggcacattt actcctgata caagagagat aagtacatcg ttgatggaaa cttctcaaat
    38461 ctatgtagat gattactcag ctgccttaac agaaagcggg gacctgctta tagccatggc
    38521 agaaggagat ttagaggaat cttgtattgt tggtacatta ggcgaattag tttctgagaa
    38581 aaaaacattt tataaaggag aatcgaggcc gacgattttc gacgcagttg gtctagctat
    38641 tgaagatctt tgttgtgcag aatatattta taacaaatta gagaaagggg ctttaaaatg
    38701 aacttagatt attacgtagt tgatgcattt gcagatgagg tgttcaaagg aaatccagca
    38761 gcggtttatg tgctagaaga atggctgcca gaaggtacga tgcaaaaaat tgccattgaa
    38821 aacaatttat ctgaaacagc atttacagtg aagaaaaacc aagaatttga gcttagatgg
    38881 tttacgccag atcgagaaat cgatctttgt ggtcacgcga cattagcaac agcatttgtt
    38941 ttatttaact attacaaaat acctgatgaa acgattaaat tttctactca aagcggtaat
    39001 ctatttgtga caagaattaa agattattat tacatggatt ttcctagtat tatgccgaaa
    39061 aaagtgccga tattgtctga atatgaagaa gcgattgggg ctaagattaa agaagcctac
    39121 ttagcacgtg atttattttt cgtattagaa gatgaaaaaa cggtggctaa attgcagcca
    39181 gattttacag ctatcaaaaa ttttgaatta ggaataggag tcatcgtgac agcagaaagt
    39241 gtgcaaaaag actttgtttc tagaaccttc tttccaaaat taacaatcaa tgaagatcca
    39301 gtatgtggat cagcacattc taatcttatt ccttattggg cagaaagaat gaataaaaca
    39361 aaattgtcag cttatcaaat gtcatcaagg ggcggggatc tagaatgtga attaaaagaa
    39421 gatcgtgtga taataggagg aaaagcagtt ttgttttcta aaggtacctc gtttatttga
    39481 tttctttcaa atgaattaaa aataaattgg tggagagtgg caaccttata gctacaatgg
    39541 ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa gtttgcgtct cattttaatt
    39601 attggtgttt gttttttatt caatcaagag taaaatgccc catactaact atcctcggat
    39661 gacttcgatt ttataaccat cagggtcaat gacaaagtag taagaaggcg cagtgccagg
    39721 taatcctttt aagtcagtaa tctcaagacc ggcagcttta tgttgctcgt gtaatttttc
    39781 tagatcatca gctgctatag cgatatgacc atagccgtct cctagatcat acgcaccatg
    39841 atcatagttg taagtcaact ctaattcata gtcatcccca ggaagtgtca aataaacaag
    39901 tgtaaatttg ttttctggaa aatcacggcg gcggctttct tcaaaaccaa atgccttttg
    39961 ataaaattcg attgatgctt caagatcttt cactcgaaca catgtgtgag ccattttcat
    40021 agattcatct ctccttttcc ttttactcaa tcataacaaa aagaagtaag gaaatagttg
    40081 atttgtgctt gttcatgaga aatttctcat gtaaaatgga tagaaaataa aactgttaag
    40141 gtggaaacta ttcatggata aaccaatttt tggtgaaaaa aagcaatttg ctgattatca
    40201 aacccgttat gcagcatata ttgtcataga aagagatcag cagattgcag taatcgaggc
    40261 tccaaatggt gctttttttc ttcctggcgg ggaaattgaa ggagaagaga cgaaagaaga
    40321 agcgattgtc cgtgagctga tagaagaaat gggaattgtg gcagaaattt cttcttatct
    40381 gggagaagct gatgagtatt tttactcgaa ttatcgtgca acttattatt ataatccagg
    40441 atatttcttt gttgctgatt cttggaagcg tataggagaa ccaactgaaa agaccaataa
    40501 aatttggtgg gtgagccctc aagaagcgct tgtgaaattg aaacgtggta gccaccgatg
    40561 ggcagtcgag agatggatcg aaaaaaataa aaactgaaag aaagcgagta gatcagcttt
    40621 ctctcagttt ttttatttat tacagtacat attttttgat tgcttcagct acaccgtgct
    40681 cgtcgtttga tgaagtgatg acatttgctg cttctttgac aagagaaacg gcatttccca
    40741 ttgctactcc tagaccagca tattcaatca tggataagtc gttctcgtta tctccgatag
    40801 ccatgatttc atcttgatca ataccaaggt gctgtgcgag attcgcaact gccgcacctt
    40861 tacttgcttc tttatttagt acttcgaagt aatagggcgc acttttgacc gttgtgtatt
    40921 tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc taaaatttct ggttcgtcga
    40981 tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc ttcaggtgtt cgatatttta
    41041 gcggcatctt gactaaactt gcttcgtgga tcgtataagc gctgatatct cgattaggcg
    41101 tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc gacacggcgt gccatcactt
    41161 cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc gatgatttta cctgatttcg
    41221 tttgttgtac gagtgccccg ttataagtaa taacgtaatc attatcttga aaaaggttca
    41281 actcgtttaa ctgttcctgg acacctggta agggacggcc agtgcataaa acgatcgaaa
    41341 caccttttgc aacagcagcg gcaatcgctt cttttacttc ttttgttact tctcttttct
    41401 catttaataa agtaccatcg atgtcgatag cgactagttt gatagacata atttcctcca
    41461 atatatttta atttatttca atcaatgcgc cattacggat atggctggca aattcttgat
    41521 aagtttcgtc aaataaatca taatgatcac gcaaggacgg atcaaccatt tcttttggaa
    41581 agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc agcaacaagc tgactaacct
    41641 gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc gatttgtgtg cgatgtctgc
    41701 cttgttctgg gcgataaaaa tcataaggta gatcgtagct agaattcaca gcagtgtaat
    41761 aaacaggatt atatccgacc ttttctacta acaaagtgag ttcttggact aattttgaat
    41821 cccggttgcc agaaaaagta gcagatttca gtggtttacg attcaaaaaa cgtttagcta
    41881 aatcatttaa aataggatca gcagattcag accattgagt aaagtaagtg cccaagaccc
    41941 catcatcaag tttgaggtat tcttctaaag taaagcttcc gtcaagaaat ggcagaagta
    42001 attgagaatg gagttcaaaa ctgctggcat cttcttgata caactcgtgt gcgcgatgaa
    42061 gcagatgctc caaaattact tccataccac gggaaacagg atggaagtac acttgcacat
    42121 acatttgata tcggctaacg atataatctt ccactgcatg catgccactc atggaaaaag
    42181 cgatcccgcc ttcataagga cgaatcacac gtaatattcg ggtaagatca aatgttccat
    42241 attctgttcc agtgaaataa gcatcccgaa gcaaatagtc cattcgatct gcatcgatct
    42301 gactagaaat catttggaca acttgtgggt tggggtaagt tttttggatc acactagcta
    42361 ccttttcagg aaaaccttct tctactcggt taagaatctg atacacttct gtttctggag
    42421 aagtgatgat ctctacagtg atggcttcat gattcgtatg gaagatatgt tcaaatgtat
    42481 gagagtaagg accatgaccg acatcatgca acagtgccgc acataaagtc accagccgtt
    42541 cctgatcatc ccagcctccg ttgcctattt tttcttttga aaaatttcga gaaaagatat
    42601 cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg agtaaaacgg ctatgttctg
    42661 ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat gcgtcgtaga cgttggactt
    42721 ctttcgaatt gatcaaatct aagattactt gatgctggac atggatatag ttatggactg
    42781 gatcacgaaa aactttttcc ataggcagca tttgatattt ataaggggtt gtcataatgt
    42841 agctccttta tgttattacg ttgttccatt ctctggattt ggttgttcca ttgctccagc
    42901 tggatttctt tttttagcca gtcggtagaa tcctgtaaat cacttgcaga atacagcttt
    42961 tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa aaaggggttg gtcaagcagt
    43021 tcttcaagtg ttgccattgt agcaggatcc actgcaggat agccatttgt accaaagtct
    43081 tcttttaaag cagattgata aaatcgcttc atgacttgcc cgcgtttttc ctgattaccg
    43141 ttgacactta agtacatcat aacagaaatc ccatttttga tccgacgctg agcgataccg
    43201 gcaaattttt tgccatggat gcttaaatca taagttccag gacaataaga atgggtgatt
    43261 tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag tcaataggag catttgctcg
    43321 taagcgtcat ctatggttat tttttttgtg tcactttggg ggaaaatcaa agaaatattc
    43381 aaaattcctt catctgatac gatccctaat ccacctgaat ttctgatcac tggagcgtaa
    43441 cctgcatctt tcagtgtttg gatcccgtca cttagaaaag gagttcgaac atccttcata
    43501 cctaagatga ctgtgcagtc atattgccag aaatgtaaaa agatttgctg gtgttcttta
    43561 gaatattccg ttaatatatc tgttaatgca aaaggggaaa agtcgttttt ttgataaatg
    43621 tgctgatcta gtcgaatcgc agggttcatt caaccggctc ctttgtataa attcatattt
    43681 tctacaatat tatactctaa aatcaaagag ttgttttgga aaaaaggttt tctgtaaaaa
    43741 tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa tgtagctatg agaagtaaag
    43801 gagaatgaat aatggaatta tcacaaggaa caccgatttc aatcaaattg cgtacaaaag
    43861 taaaacagca aggggaagtg caagattttt attttgattt gaaagggcaa atggtgacaa
    43921 ttggcgatac gctgtacata cgttataaag agattcagga ggagacgggc gatgagattc
    43981 ctgtaacgat caaattagtt ccagatggtc atgtacagct gatccgtgca ggagaaatgc
    44041 ggatgcgttt gaaatttggc tataaagaac ggttagaaac atcctatcga acaccatacg
    44101 gtatgatcca gattgctact tttacgaaag aactgcatgt tagtttgaaa gaccgtccca
    44161 caggagggaa ggttcgaatc gattatgatc tgttcatggg accggaaaaa atcggcgaat
    44221 attatttgac cttggatttt acagcgtaaa tataaaagaa aagtttgatt ttttgtgtct
    44281 gattttgtat gataaggagt agactgtgaa aggacgtgtc ctgattggaa attagtgtat
    44341 ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt agcacacgcg atcttagaac
    44401 aacgtggcga tgtcatggat ttttctgatt tagctaacca gatccaaaac tatcttggga
    44461 aatcagacag tgaaattcgt gattcactcg cacaatttta tactgacttg aatattgacg
    44521 gcagctttat ctcattaggg gataatcgtt ggggcttgcg ttcatggtat gctatcgatt
    44581 cgatcgatga agaagtaaac catggaatgg acgaagagga tgaagatact ccacgtcgcc
    44641 gtaaacgcaa gaaagtcaac gcctttatca atgatgatga agacgcgatc gattacaatg
    44701 acgatgaccc agaagataca gatctgacag aagaagatga cgatgatctg tttgatgacg
    44761 atgatgatga agatgaagaa atcgctgctt ataattctga cttgcaagaa atcggagcag
    44821 atgacccgac agatgatgaa gaagacttgc caggaaatat cgaagaagat ttaagtatca
    44881 tagacgaaga cgatgatgat gaagattatg aagaggaaga attttctgac tctgaaaaag
    44941 aataaacatt attttcttac gagaggttgt gacagagacg atcagcctat cagaataagg
    45001 aggtgtgaca cgatttttgt cacaccttct tttttgtata taaaaatccc aagtaaatac
    45061 ataaaaaata aaaaaattga aacttttagc gcaccttgta cgtcatatta agtgaggcag
    45121 aaaggagtgg agaatgattt ttactggata taagaaaaaa cggcagatcg aaaaataccg
    45181 tcacctgctg aatggactca tcgaaactga ttatcaaaaa atgttccgta tcacgatgaa
    45241 ttacgtacat gataaagaag aggccttaga tgtgatgcaa gatagttttc ataaagccct
    45301 gaactatttt gaaaaagaaa aagagatcga gcattttagt gcatggtttt atcggatatt
    45361 gatccgcacg gcattagata gttggcgcag acaaaaaagg acgcctgtgg acttatttga
    45421 aattcaagat atcccccaac agttaccttt agaaacttcc gttgcagagc tccatatgat
    45481 tttagataaa atagacagtc cggaaaaaga gattctgatc ctctactttt ttgaaggatt
    45541 tcgtttgaaa gaaattgcga ctatcttgga tatgaatgaa aacacagtta aaacgaaaat
    45601 gtaccgctca ttgaatgctt tacgtcaaat cctagaatga aggagagaag agtgccatgt
    45661 attcccaaaa agaaaattta aaaaaactaa agaaaactta taaagaacaa gcaattccca
    45721 aagggcttaa agaggagatc cgccagcgtt ttattatcga agagcaatca tttataaaag
    45781 caaagcggag aaagagacgt ctccaagggt tcggagtatc tttggctatc gtaatgatta
    45841 caacaagtag ccttcttttt aatagtcaag ttcgaagctt tgcagaagat ttacccatct
    45901 taggatcagt cattgaattg atcttaggcg aacgctttac tgatcgatct gaaaaaatcg
    45961 atattcaggt accgaagatc aatacacaaa atgagaaaga aaataaaaca attcatggat
    46021 taaatcaaaa atactttcgc gaaggacaag cagattttga aaaagctgaa aaagaatatg
    46081 gaaattttga aacggatcat taccaagttt tgggagatta tcagaaaatc gtagacgata
    46141 accggtttct cgtgattgaa aggcagatca cccaaacagc agcagatagc catgttgaaa
    46201 aaagatacga cacaatcgac aagaaaaaca gtgtccagct ctcacttcca cttttattca
    46261 aagatgacac ctatctatct gttttgacaa aagaagtcaa acggcagatg gccgaacaag
    46321 tcaaggaaga tccatctaag tactattgga ctgagcaaga tattcaagaa gggacgatag
    46381 aaaaaccaac acttgttacg cctacacgaa gtttttattt gaacaaagag catcaattag
    46441 ttttgacttt ttctcaatac gaaattgctc ctggttatat gggtacacca gaatttgtca
    46501 ttcctaagtc cgtgacaaaa acgattttgg catcggaaga ttatttggat cattagacga
    46561 gaagtagttg cgactagcag tttcaatttt caagtaagta caagtaaata ataagaagag
    46621 aaaaagagaa agaagagata tagtgaaaag aaattattta gttatcagta ttattgcttt
    46681 atccgtgctt ttgtttttcg aaattacttc ggtggcctcc ttgatcaccg aagtaaaagg
    46741 acaaacacac tcttcggagc attcgtcaca atctttcaaa caatcagaac aatcaaccat
    46801 taaaagccaa tcaactgaaa cagctaacgt caaagaagcc attcaagaat cagcggaaaa
    46861 ggtacaaagt cagtcatcac aatcaacaca agacagtaca aaaacgagcc gagatccggc
    46921 tgtcaaaagc gtcgcaatca gctttgatga tggaccagga gcgaccacaa caccgcagct
    46981 tctgcgaatc ttaaaagaaa aaaacgtgca tgccactttt ttcgtcttag gagaaaatac
    47041 ggcgcagcat cctgaaatcg tgaagcagac agctgaagca gggcacgaga ttggcaatca
    47101 cacatacgat catcagaatc tggccactct ttctgcccag tcgatgacgg aggaagtgac
    47161 gaaagcagac actgagatca aaaaagtcac tggcaaaacc cccacttttg ttcgtccgcc
    47221 ctatggttct gtcacgaatg taggagcaac gatcatccag cgaccgatca tcgaatggtc
    47281 tgttgattca gaagattgga agacaagaaa tcctgatttg attttgcaga agatccaagc
    47341 aacagtatac gatggggcaa ttattttatt ccatgatatc tatcctgaaa cgattcgtgc
    47401 cgttcctcaa gtgatcgatt atctgaaaga gcaagggtat cgcatcacga cagttgggga
    47461 tttattaggg catccaacgg ctgtcgagaa ctattatggc agaaacgatc atcggccagt
    47521 tcagtaagtg agacaagtat ctttaataaa taacacattt taataagtag ttgaacacat
    47581 aactacctta cttgcaaagg cttaacaata tctatacagg tacaacaaaa aactgtccac
    47641 caattttctt tggcggacag ttttcactta aaaataaaga agcaactatt cgtaaattat
    47701 gctaaaatag cagtagaacg cgtcctgagg gaatcgaacc cccgtctcaa gaaccggaat
    47761 cttacgtgat atccactaca ctaaggacgc aagcactggg ataattgtaa ctaaacagat
    47821 aaaaaattac aaggggattg gacaaaaaaa tgaaatttga gcaaaatttt tcacaaaaac
    47881 agcagcagac acagaaacta gccatgacgc aacagttgca acagtccatc caagttttac
    47941 aatataacag tgaggagctg ttggcattcg ttgagaatca agcaatggaa aatccattgg
    48001 tggaagttgt tgagccggaa tggcagccag attacataaa agcttcttca tcttcttatg
    48061 aaggagaaga gacaaattac ctaaatcaga ttcctgatac aaaagggtct ttatttgatt
    48121 cattgatcga acaagtccat ttgaattacc gagatacttt tttgaggaaa atcgttcttt
    48181 atcttgtaga atacatcgat ttgaacggat ttttgacgat tagtttagaa gaagccggaa
    48241 aagagattgg agctacgcct atccaaatgc tcgatgcgtt gactctgatt cagcaattag
    48301 aaccagctgg agtaggtgca cgtagtttgc aagaatgtct catgcttcaa acagaacgag
    48361 atgattatgc gccggaatta gcttatatcg tgttggaaga atgttttgag gaactggtgg
    48421 agcgaaaatg gaaagaaatc gcacaacgtt tttctgttga tctacatgct gttcagcaga
    48481 tattcgacta tttacaaacg ttgagtccaa gccctggccg gattttcgac cgtagcagcg
    48541 aattgtacat tattcctgat gtccgggtat tagtggacga cgataaaaat gtacaagtta
    48601 tctcaaaccg aaaaaatcag ccaaatatac gctttcaaga gagttacttc aaacaaatgt
    48661 cacaacaagc agataaagaa actgaaaatt atttaaaaga acggaaacag gaattcgaat
    48721 ggttgaagaa aacgatccta caacgtgggg atacaatttt gcgggttgct caagtcattg
    48781 tttcccggca aaaagctttc tttatcgaca aggaacgtcc aatcaagcct cttacgttaa
    48841 aggaagttgc aactgagata gatgtgcatg aatccacggt cagtcgtgca gtcaacggga
    48901 aatatttgga aacagacttc ggtgtatttg aattaaaaaa atttttcact acacgaattc
    48961 ctacgaatag tacagaacag acagaagacc tttcagcaga tacagcgaag aaaaagctgc
    49021 aggaattagt cgatcaagaa gacaaaaaca agccgttgtc tgaccagaaa ttggttgaat
    49081 tgctgaaaaa agatgagata gcgatctcaa gacgaacagt agcaaaatat cgtgatttat
    49141 taggaatccc gagttcatca aaaagaaaac gttatgataa ctaatcagtc aaaagactag
    49201 aaaaagtatt tcactttttc tagtcttttt agttgaaaaa cgagagcaaa actgttagac
    49261 ttacagtcgt gaggttgaga tttcccttta ttgatgtgaa attcgaatag ttaatttctt
    49321 tttttatcgt atatgggtcg ttttaagtct acattggacg taaagtgtcc aactaaggag
    49381 atcgctatgt tggatgaaat aaaaatgatt gaatctgttg caccagatat tatcgaagtg
    49441 ttgcaagaac gctataaaat tttacgaaat atctattgga tgcagccgat tggacgtcga
    49501 agtttatcgg aaagtatggg gatcacagaa cgagtattgc gaacagaaac agatcttttg
    49561 aaaaatctga atttgatcga cacctcaaaa agcggtatga cgctgacaac taagggcgaa
    49621 gaagtttatc aaagccttga aaacttcatg gatcagcttt taggtacgca tcaaaccgaa
    49681 caacagttgg cagaatattt tggtaaccaa cgctgtatcg ttgtttcagg aaacagcgaa
    49741 gagcagacca aagtggctga tgcattcggc gaagcgctga gcgaagccct agaccgacta
    49801 cttccagaag gcgagaatat catcgctgtg atgggaggaa ctactatggc tgtggtagcc
    49861 gaacaattgt ctaatctgga aaataagaaa cggcataacc tgtttgtccc tgcaagaggc
    49921 gggattggtg aagcgataac tgtacaggca aattctgtca gtgcaagaat ggctgctaaa
    49981 gcaaacggta accaccgtgc actttatgtt ccggaacaat taagtctggc aacttacaat
    50041 tctttactca atgagccatc tatccaagaa gtcttaaact tgattagtga agcgaattgt
    50101 gttattcata gtattgggcg tgcattacac atggcagcac gtcgtaagat gactgaaaaa
    50161 gaattagtca tgctgaaaca agcaaatgct gttgcggaat cttttggcta ctttttcaat
    50221 gaaaaaggtg aagtcgttta taaggtccct agaatcggca tagaattgca agatttggaa
    50281 aaagtcccga tcatcatggc aatcgcgggc ggaaaatcaa aagccaaagc aattcgagct
    50341 tatatgaaaa atgcaccaaa acaaacgtgg ctcatcactg atgaagctgc tgcaaatgag
    50401 attttaaaag gggtaaccct ttaaaataaa tatttttttg attttcataa ggaggaaatc
    50461 ttaaatgaca gttaaagtag gtattaatgg ttttggacgt attggacgtc tagcattccg
    50521 tcgtatccaa gatgtagatg gaatcgaagt agtagcaatc aacgacttga cagatgctaa
    50581 aatgttggca cacttgttaa aatacgacac aactcaagga cgtttcaacg gaacagttga
    50641 agttcatgaa ggttcattca acgtaaacgg aaaagaagtt aaagtattag ctaaccgcaa
    50701 cccagaagaa ttaccatggg gcgaactagg cgtagatatc gttctagaat gtactggttt
    50761 cttcacttct aaatcagcag ctgaaaaaca tttaacagct ggtgctaaac gcgttgttat
    50821 ctctgctcct ggcggaaacg atgttccaac aatcgtttat aacacaaacc acgaaacatt
    50881 aactggtaaa gaaacagtta tctcaggtgc ttcatgtact actaactgtt tagctcctat
    50941 ggctaaaaca ttgaacgaca aatttggtgt tgttgaagga ttaatgacaa ctatccacgc
    51001 ttacacaggt gaccaaatga ctctagacgg acctcatcct aaaggtgact tccgccgtgc
    51061 acgcgctgct gctgcaaaca tcgttcctaa ctcaacaggt gctgctaaag ctatcggttt
    51121 agtaattcct gaattgaacg gtaaattaga cggagctgct caacgtgttc ctgtaccaac
    51181 aggttcatta acagaattag taacagttct tgaaaaagaa gtaactgttg acgaaatcaa
    51241 tgcagcaatg aaagaagctt caaacgaatc ttacggatac aacacagacg aaatcgtttc
    51301 ttcagatatc gttggtatga cttacggttc attatttgat gctacacaaa ctaaagtaat
    51361 gacagttggc gacaaacaat tagttaaaac tgttgcttgg tacgacaacg aaatgtcata
    51421 cactgcacaa ttagtacgta ctttagaata cttcgctaac ttataagata gacaaacgta
    51481 caagtgtaaa ttatagacat gtacatttaa aagcggggaa gcaatcgcgc ttcttccgct
    51541 ttttttgata ataagtttat t
//
LOCUS       DACEKG010000011        49891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEKG010000011 DACEKG010000000
VERSION     DACEKG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.011"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..86)
                     /locus_tag="IW664_000563"
     CDS             complement(<1..86)
                     /locus_tag="IW664_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0847951.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDIL"
     gene            complement(88..285)
                     /locus_tag="IW664_000564"
     CDS             complement(88..285)
                     /locus_tag="IW664_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0847952.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(679..2406)
                     /locus_tag="IW664_000565"
     CDS             complement(679..2406)
                     /locus_tag="IW664_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0847953.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2399..3592)
                     /locus_tag="IW664_000566"
     CDS             complement(2399..3592)
                     /locus_tag="IW664_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0847954.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3779..4423)
                     /locus_tag="IW664_000567"
     CDS             complement(3779..4423)
                     /locus_tag="IW664_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0847955.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4517..4651)
                     /locus_tag="IW664_000568"
     CDS             complement(4517..4651)
                     /locus_tag="IW664_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0847956.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4653..6785)
                     /gene="feoB"
                     /locus_tag="IW664_000569"
     CDS             complement(4653..6785)
                     /gene="feoB"
                     /locus_tag="IW664_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0847957.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6782..7255)
                     /locus_tag="IW664_000570"
     CDS             complement(6782..7255)
                     /locus_tag="IW664_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0847958.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7656..7880
                     /gene="nrdH"
                     /locus_tag="IW664_000571"
     CDS             7656..7880
                     /gene="nrdH"
                     /locus_tag="IW664_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0847959.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8039..10198
                     /gene="nrdE"
                     /locus_tag="IW664_000572"
     CDS             8039..10198
                     /gene="nrdE"
                     /locus_tag="IW664_000572"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0847960.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10248..11213
                     /gene="nrdF"
                     /locus_tag="IW664_000573"
     CDS             10248..11213
                     /gene="nrdF"
                     /locus_tag="IW664_000573"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0847961.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11302..12441)
                     /locus_tag="IW664_000574"
     CDS             complement(11302..12441)
                     /locus_tag="IW664_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0847962.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12750..13463)
                     /locus_tag="IW664_000575"
     CDS             complement(12750..13463)
                     /locus_tag="IW664_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0847963.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13717..14418)
                     /gene="nagB"
                     /locus_tag="IW664_000576"
     CDS             complement(13717..14418)
                     /gene="nagB"
                     /locus_tag="IW664_000576"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0847964.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14652..16184
                     /locus_tag="IW664_000577"
     CDS             14652..16184
                     /locus_tag="IW664_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0847965.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQINWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16375..17577)
                     /locus_tag="IW664_000578"
     CDS             complement(16375..17577)
                     /locus_tag="IW664_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0847966.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17659..18348)
                     /locus_tag="IW664_000579"
     CDS             complement(17659..18348)
                     /locus_tag="IW664_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0847967.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18454..18822)
                     /gene="rplL"
                     /locus_tag="IW664_000580"
     CDS             complement(18454..18822)
                     /gene="rplL"
                     /locus_tag="IW664_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0847968.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18875..19378)
                     /locus_tag="IW664_000581"
     CDS             complement(18875..19378)
                     /locus_tag="IW664_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0847969.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19788..20477)
                     /gene="rplA"
                     /locus_tag="IW664_000582"
     CDS             complement(19788..20477)
                     /gene="rplA"
                     /locus_tag="IW664_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0847970.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20581..21003)
                     /gene="rplK"
                     /locus_tag="IW664_000583"
     CDS             complement(20581..21003)
                     /gene="rplK"
                     /locus_tag="IW664_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0847971.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21346..22014
                     /gene="sdaAB"
                     /locus_tag="IW664_000584"
     CDS             21346..22014
                     /gene="sdaAB"
                     /locus_tag="IW664_000584"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0847972.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22027..22899
                     /gene="sdaAA"
                     /locus_tag="IW664_000585"
     CDS             22027..22899
                     /gene="sdaAA"
                     /locus_tag="IW664_000585"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0847973.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22990..24009)
                     /locus_tag="IW664_000586"
     CDS             complement(22990..24009)
                     /locus_tag="IW664_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0847974.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24182..25069)
                     /locus_tag="IW664_000587"
     CDS             complement(24182..25069)
                     /locus_tag="IW664_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0847975.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25146..25691)
                     /gene="nusG"
                     /locus_tag="IW664_000588"
     CDS             complement(25146..25691)
                     /gene="nusG"
                     /locus_tag="IW664_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0847976.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25792..25962)
                     /gene="secE"
                     /locus_tag="IW664_000589"
     CDS             complement(25792..25962)
                     /gene="secE"
                     /locus_tag="IW664_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0847977.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25986..26138)
                     /gene="rpmG"
                     /locus_tag="IW664_000590"
     CDS             complement(25986..26138)
                     /gene="rpmG"
                     /locus_tag="IW664_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0847978.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26300..26944
                     /locus_tag="IW664_000591"
     CDS             26300..26944
                     /locus_tag="IW664_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0847979.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27000..27503)
                     /locus_tag="IW664_000592"
     CDS             complement(27000..27503)
                     /locus_tag="IW664_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0847980.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27516..27562)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28100..29065
                     /locus_tag="IW664_000593"
     CDS             28100..29065
                     /locus_tag="IW664_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0847981.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29164..30336)
                     /locus_tag="IW664_000594"
     CDS             complement(29164..30336)
                     /locus_tag="IW664_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0847982.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30323..31468)
                     /locus_tag="IW664_000595"
     CDS             complement(30323..31468)
                     /locus_tag="IW664_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0847983.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31482..32406)
                     /locus_tag="IW664_000596"
                     /pseudo
     CDS             complement(31482..32406)
                     /locus_tag="IW664_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32523..33602
                     /locus_tag="IW664_000597"
     CDS             32523..33602
                     /locus_tag="IW664_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0847984.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33634..34227
                     /locus_tag="IW664_000598"
     CDS             33634..34227
                     /locus_tag="IW664_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0847985.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34337..34564)
                     /locus_tag="IW664_000599"
     CDS             complement(34337..34564)
                     /locus_tag="IW664_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0847986.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34667..37255)
                     /locus_tag="IW664_000600"
     CDS             complement(34667..37255)
                     /locus_tag="IW664_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0847987.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37275..37934)
                     /locus_tag="IW664_000601"
     CDS             complement(37275..37934)
                     /locus_tag="IW664_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0847988.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37983..38486)
                     /gene="trmL"
                     /locus_tag="IW664_000602"
     CDS             complement(37983..38486)
                     /gene="trmL"
                     /locus_tag="IW664_000602"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0847989.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38707..39573)
                     /locus_tag="IW664_000603"
     CDS             complement(38707..39573)
                     /locus_tag="IW664_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0847990.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39682..40323
                     /locus_tag="IW664_000604"
     CDS             39682..40323
                     /locus_tag="IW664_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0847991.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40385..41746)
                     /gene="mgtE"
                     /locus_tag="IW664_000605"
     CDS             complement(40385..41746)
                     /gene="mgtE"
                     /locus_tag="IW664_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0847992.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41764..42663)
                     /locus_tag="IW664_000606"
     CDS             complement(41764..42663)
                     /locus_tag="IW664_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0847993.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42665..43462)
                     /locus_tag="IW664_000607"
     CDS             complement(42665..43462)
                     /locus_tag="IW664_000607"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0847994.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43440..44144)
                     /locus_tag="IW664_000608"
     CDS             complement(43440..44144)
                     /locus_tag="IW664_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0847995.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44275..44850
                     /locus_tag="IW664_000609"
     CDS             44275..44850
                     /locus_tag="IW664_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0847996.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            44968..45645
                     /locus_tag="IW664_000610"
     CDS             44968..45645
                     /locus_tag="IW664_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0847997.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45724..47532)
                     /gene="pepF"
                     /locus_tag="IW664_000611"
     CDS             complement(45724..47532)
                     /gene="pepF"
                     /locus_tag="IW664_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0847998.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47708..48706)
                     /locus_tag="IW664_000612"
     CDS             complement(47708..48706)
                     /locus_tag="IW664_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0847999.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48932..49585)
                     /locus_tag="IW664_000613"
     CDS             complement(48932..49585)
                     /locus_tag="IW664_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0848000.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14934 a        10472 c         8763 g        15722 t
ORIGIN      
        1 tcaagaatat cagaatattt aggatcttta cttatagata tatcattata taattcatga
       61 acaatttgct tagcattttt tttcatttta gcacttccct ggaattgctc cacctaaaaa
      121 accacctata gacattcctg caatacaagt agttgccttg gcccaatcga ccgtacattt
      181 atttttagtg caatacactc catttccata atattttcca ctatgagtgg tacccccata
      241 gataagttgt gtctctttaa tagacaaaat ttttaaatgt ttcataatct aatcacccct
      301 aaattaatgt ttttagacta ttattctaac attcattaaa tttcaaaaaa caattccttc
      361 ttgaaacgta gcaaaataat tcctgaataa gaatttaatc acttttaagt atataaacta
      421 tgtatattct ttaatatttt ttgagaatgc acttcgtaca taataaactt taatcacatt
      481 agtctttcat acctacgatc gatctctttg actcgcaccc ataaattcat aatagtttgt
      541 cggcgtcttt ggacgtttcg aagatgagct acgcattctc tttttttata gacaaaaaag
      601 atagagacga acatggagct tccaaatttg gatatttgat ccaattttgg aagctccatt
      661 tatttcgtgt ctgtcttttt attttttcca gctttcgact aattcttggt tctctttgat
      721 ccagtctttt gctgcttgct gtggatcttt tccattgttg atttccaaca taactttttc
      781 catatctttt tctgaccaat ggaaattgtc taatactttg taagcttctg gctggtcttc
      841 tttcaatcct tttcgaacca tcgtatggat ctgctcttcg cttcccattc cgttttcagg
      901 atcttctaag tatttcagat catatttggc aaacatccaa tgaggtgtcc atcctgtaac
      961 tacaattggt tcgtgttttt tgatcgcttg acctagtgca acggtcattg ctcctgaaga
     1021 agatgtctct actttccagt ctttcaagtt gtcatatttt tggatcgtgt tttcagcagc
     1081 cgtaactaca ccagctcctg gctcgattcc tatgattttc ttacctgcct gatcggttaa
     1141 ctcatcgatc gaatttacat ccatgtaggc tggcactgct aaaccaactt ttgctcctgt
     1201 caaattggca cctaggtctt cgacttgatc tttatattgt gcatattgtg aaccatgagt
     1261 tttaggtagc caagctgaaa ccatggcatc tgactcgcca tttgaaacag atttccacat
     1321 gattgaatta tctaatggcg tcatcgttac atcatagccc atttgtttca atacttcacc
     1381 gactacattt gttgaagcaa cttctgtatc ccattcaaca taagataagt tgatacgttt
     1441 tgtttctttg gcagatgaaa aactccctat accaatcgca cctaagatca ctactactac
     1501 agcaccaatg atcagtcctt gacgtttttt acttttctgt gttgttgctc ctgcagcttt
     1561 tttattgttt ggctggttca aatgctgtgt aaaacggtca atgatgattg ctaagataac
     1621 taatgcaacc ccgttgacaa atccattccc gacttgtgct ctttgcaatg ctgataatac
     1681 gccgcgtcct aagccgggtg caccgatcat agaggcaata acgaccattg aaagtgacag
     1741 cattgttgtt tgattgatcc ctgccatgat cgtactttta gctaaaggca attctagttt
     1801 gaatagtttt tgacgacttg tgctcccgaa tgaatcagaa gcttctacta attctttagg
     1861 aacttgtcga atccctaaat tcgtaaaacg aacggttggt ggcagagcaa agatgactga
     1921 ggcaaatacc cctggaacca tcccgatacc gaaaaatgca actgctggaa tcaaataaac
     1981 gaatcctggc attgtctgca taaagtcaag gatcggtttg ataatacttt gggctttact
     2041 gctttttgcc atcaagatcc ctaatggtac cccaatgatg atcgagatca cactagataa
     2101 aagaacaagt gtgacagtat tcatcaaatc attccataga ccttggttat aaataaacca
     2161 tagaccgatt aatgtgaaca acgtaagtcc tggacgtttc ttagaaataa agaaagctgc
     2221 taccgtcaat aacaagataa acaaaggagc tggaatcact aacagtaaat ttgtaatccc
     2281 cgacatcact gcctgtccaa tcgtttgtaa aaatgaaaat aggccagaaa aagttgtcgt
     2341 aaaccattct gtgatatttt ctacccagtt tgccactggt aattgatagt tcaatagatt
     2401 attcatgttc actcacctcg cttgtttctg ctaatgcttc aatcacacta cctcggatga
     2461 cgacaccttt taattttcca tcctctacaa ctgcaagtgg agtcggtgag tcataaataa
     2521 tattgaaaat atcacttacc accatgtctt tcccaatcac cgtcacattt tcatctagga
     2581 agtcgatcaa tggacgtttt tgcttcctag cttctaacgc ttgatctgca gtgatgatcc
     2641 ctttcaggtg tctctttctg tcaacagcca tcagcatgct gacttcttca ttacgcatcc
     2701 ttgtcaatgc aacgtttggt ccatcagatt cgatattcgt cgtcaatgca ggtaccatga
     2761 tgttttgagc cgtaagcact ttggaacgat cgacttcttc cacaaattca cgtacataat
     2821 catttgctgg attggtcaat atttcttcac ctgttccgat ctgcatgatt tcgccgtctt
     2881 tcatcaaggc gattctgtcc ccaatccgta aagcttcatt caaatcatgg gtaatgaaaa
     2941 taatcgtttt ttgaacattg gcttgcaggt ccagtaattc gtcttgcatt tctcgccgga
     3001 tcagcggatc aagagccgag aacgcttcgt ccatcagcaa gatctcagga tcatttgcta
     3061 aagctctggc taggccaacc cgttgttgca tcccaccaga taactgacta ggatattggt
     3121 ctttaaatga aagcaaacta gaattttcta atgctttttc tgctttttct tgtcgttctt
     3181 ctttaggtac accacggact tctaacccgt attctgtatt ttccaagatc gttcgatgtg
     3241 gaaacagacc gaagttttgg aaaaccatat tgattttatg acgacggact tctcgcaatt
     3301 cttctttgct taacttcgat acatcttctc catcaatata gatactgccc gaagttggct
     3361 caatcagacg attcaataga cggatcaatg tggatttccc actcccggaa agccccatga
     3421 tcacgaagat ttctccttct tttacgtcaa aattcacgtc atacacacca accgttgcac
     3481 cggttttctt taaaatctcc gttttcggct ggtgatcatt catcatatct aaagcggctt
     3541 gtgtcttttt accaaatatc tttgttaaat gttttacttc tacttttacc aaacaaattc
     3601 tcctttttta tgtaaattca tttttctttc tgaaaaaagt gaccacacta tgttaacatc
     3661 atgtagtcac tttgtcaaaa atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc
     3721 taaatctttg taaataaaag gtttagccaa gttaatattt ttttaatatt tcacacaatc
     3781 attctgcaag ctgatgctcc gggtaaaaaa agtttgtcat acgctgtagt gtatattcat
     3841 ttccaataaa atgaacgatg tcccctgccg taaatttagc ataggggcca ggtgaaacaa
     3901 gcagttcttc attatgagaa attgcgataa ctgtggcgcc agtcgcctgc cagatattca
     3961 actcgctaat tgaacggtcc agatgcattg ctttttctga taaagataat tcaaacggaa
     4021 gtaaaggatt catcttatgt attcgcttcg tttgattgac cagctgatcc aataattgag
     4081 aaaaatgatt cagctcatct tgttgcttct ccacacttgc catgatttct gcttttgtat
     4141 cttggatgga ttgtacatct tgatattgca ataggaaatc ttttgctttt tccttcgaag
     4201 caacaaatgc cccacttcca tgccgtactt ccatgattcc aagatctacc agcacattga
     4261 tagccttgcg ggtagtctca ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat
     4321 ggatcttctc acccacttta tatcggtttt caacgatacg ttcggctaaa tcaacagcaa
     4381 tctgctggta gcgcgggcgg acgatttttt tctttccaga cattttcttt cctctttctt
     4441 tttttacttt ccctcatctt actatgctct agcaaaataa gcaaaaaagg ggagaatagc
     4501 ctcttgcaac gactgatcat cgcttgatct gttccttttt tactgggcaa gctgtttgac
     4561 agtcatcaca attcttaccg gattttaaac gagtatagac aattgcgcct gcacttccaa
     4621 agattaacac acttaataag aatgtcgcca tcctattcct ctccttctaa tgacaatata
     4681 gtataaaact ggttcttgga ttttggctga cggaacaaaa cataaacgag tcctatcatc
     4741 agtaaaatag caagatacgt ccctaacgct acctgtccac cctccaataa gtgtcccaac
     4801 tgataaatca caaaactaac taaataagct aaaccgcatt gatagcctat ggcgatccaa
     4861 gtccatttct tggcattcat ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca
     4921 caaagtaaat tgaaggctaa caatgaataa gctgctgcgg gagtatatgt gtgttgtagc
     4981 acttgccaga tttctctgcc attttcggat acttccgcat gaccaaacaa aataccaaat
     5041 gtgccaataa tattttcttt agcgaccaat cctgtaatcg ctgcgactgt tccttgccag
     5101 tttccccaac caagcggctg aaaaacaact gccaggattc gcccgaaaaa ggctaagata
     5161 ctttggtctt cagaaacagg ttgagcataa aaattataag aagagctgaa ccagatcacg
     5221 atactagaaa caaagataat cgttcctgct tttttaacaa aagacttgct gcggtcatag
     5281 gtttgtctta ggaccgctga ccattgagga aaatgatatg cgggcaattc catgataaaa
     5341 ggagctgtat ctccagcaaa caaacgtgtt tttttcaagc tgattccaga gaaaataatt
     5401 gcagtcatac caagaaaata ggcagaagga gctacccaag attgatgagg aaaaaaggcc
     5461 cctgctacta aggcaataat cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg
     5521 atcgtcaaac gacgatcttg ctcgttttca atcgtgcgac tggccatgac gcctggcact
     5581 ccacatcctg tagcaatcag cataggaatg aaggattttc ctgaaagacc gaatttcctg
     5641 aaaagtcgat ccatgacaaa agcgatcctc gccatatatc cgcagtcttc cagaaagctc
     5701 aagcataaaa acaatataat caattgcggt agaaatccta acactgctcc cacgcctgcg
     5761 atgatgccgt tcaagatcag ctcctgcatc catcccgcta cttgccattc agctaacaat
     5821 ctgccaacat gttctggtaa ccatgtacca aacaatgtgt cattgatcca atctgtcccc
     5881 attgttccta ctgtttgaat agccagataa tagatcagcc acatcacaaa tgcgaagatc
     5941 ggcaaggcta accaacgatt cgttgcaatc tgatcgattt tgtcactcat cgataattgg
     6001 aaagaatcat ttgacacaac acagagtgta catagttggg taataaattc ataacgttca
     6061 ttgaccagaa tcgtctcact atcgtcgtcc aatagtttct ctgttaactg gatcagttgc
     6121 tcgatctctt tttgctgaga aactgctatc tcgtaatcct ctcttacctt ctgatctcgt
     6181 tcaaaatatt tcattgcata ccagcgtttc ttgccgcttt gttctcctag tacatcttca
     6241 atttcatgta aagctgcttc taaacgtgga tcatatgtac agggaacagg aatagaaggc
     6301 cgcttttttg cagactcctg aatcttttcg attagttttt cgattccttg ctttttcaat
     6361 acgctgattg gtaaaactgg tacaccaaga ccataggaaa gcttttctag attgattttt
     6421 tttccttctt ttttgatgac atccatcata ttcagaccaa tgatcatagg aatgtctaaa
     6481 tccaataact gcgtagttaa ataaagattt cgttctaaat tggtagcatc aataatgttg
     6541 atgatcagtt caggctgttc ctgaaccaaa taatctcttg caatcacttc ttctggtgta
     6601 taaggagaca aagaatagat gccgggtagg tcctgaatat gaatgttcgg attttttttc
     6661 aacgatcctt cttttttttc tactgttacg cccggccaat tccctacata ttgattcgaa
     6721 ccagtcaaga gattgaacaa gctagttttc ccgctgtttg gatttccgac taacgcgata
     6781 ttcattcttt ttcctccctt tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca
     6841 gttcataccc tcgcactgtc agttcgattg gatctcccag aggggctagt tttcggataa
     6901 ctatttttac atttcgcgtc aatcccatat ccattaaccg tcgcttcact gcgccttgtc
     6961 cgtggattgc tacgacgatc ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc
     7021 tttcagagga agcagtcttt ttattgacaa agatttgacc tagtatttcc cgacttactg
     7081 ccactcgatg actttttacc agtaaaatgg cattgttgcc tgataaattg acgacgacga
     7141 ttttacttcc ggcaacgatc ccgatatttt gcaaatggcg ttttgttttt tcttctgttt
     7201 cgattcgttc gacttgataa acgaaattct gctttgcctg cgctaaggtc tgcatattga
     7261 ttcctctctt gtgaaataaa tcatgtatta aaatcattca ttatatgttc taattttctt
     7321 acgtgttacg caaataaatg agaatcattc tcacatagaa tactctcggt aattgagaat
     7381 gtcaattata cttgtgatat tatgagcgaa tctttagtat tgatagataa aaatgagaaa
     7441 aaaatatttc ttcatagttt tccacaattt tttctctatt aaatttgcta ttttcccaca
     7501 cgatacttgt tttcagctct tttttcaaaa acacaaaact tagtattcca cagtttccac
     7561 aacacaatat gtagtgtttg ttgtttgact tttttactaa gagttttata ctatctatta
     7621 tagataataa gaatgtgtta gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa
     7681 ataattgtat gcaatgtaag atggtcaaac ggtttttaac agaaaatcaa attgcttttg
     7741 aagaagtcaa tatcgatgaa cagcctgaag cagtagactg gttgaaagca caaggattcc
     7801 aaagtgttcc tgtcattact tctgatgcag aaacagttat cggattccgt cctgatcagt
     7861 tgcgtaaact tgccagctaa catgactaaa agaatcaaaa cgtgatacat ggatttgaag
     7921 ggcttgagac agcaccagtt tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa
     7981 caatgcaatg atttgcaatc aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat
     8041 gagtcttaaa aatttacaag atgtcagtta tttcaagctg aataatgaaa tcaatcggcc
     8101 agtcaatggt caaatccctt tgaataagga taaagaagct ttagttgcct tttttgagga
     8161 aaatgtcaaa ccaaatacga tgacttttct atctattatg gaaaaaatca attacttgat
     8221 cgatcaagaa tatatagaag cagaattcat tcaattgtat tcaccatcat ttattgaaaa
     8281 gctatatcaa tttttggacg agcaacattt ttctttcaaa tcttttatgg ctgcttacaa
     8341 attttattct caatatgcgt tgaaaaccaa cgacggttcc gcttatctgg aaacttatga
     8401 agatcgagta gccttcaatg cgctttactt tgcgaacggg gatgaagaat tagcacttgc
     8461 tcttgcagac gaaatgatcc atcaacgata tcagccagct acaccttctt tcttgaatgc
     8521 tggacgtaaa cgtcggggcg aattggtgtc ttgtttcttg actcaagtca ctgacgatat
     8581 gaactctatc gggcgctcaa tcaactctgc tttgcaattg tcccgtattg gtggcggcgt
     8641 agggattact ttatcaaatc ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc
     8701 tgcaagtggt gtcgttccag tcatgaaatt attcgaagat agtttcagct attctaatca
     8761 attaggtcag cgtcaaggag ctggcgtggt ttatttgaac gttttccatc cagacattat
     8821 tgctttcctt tcagcaaaaa aagaaaatgc agatgaaaag attcgcgtaa aaactctttc
     8881 tcttggcgtg atcgtacctg ataaatttta tgaattgaca cgtaaaaacg aagatatgta
     8941 tttatttagt ccatatagtg tcgaaaaaga atatggtgtc ccattctctt atgtaaatat
     9001 tacagctgag tatgatcatc tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc
     9061 gcgtgatctt gaaaatgaaa tctcaaaatt acaacaagaa tctggctatc cttacgtaat
     9121 caatattgat acagcgaacc gcgagaatcc aatcgatggc aagatcatca tgagtaattt
     9181 atgttcagag attttacaag ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga
     9241 agtattagga acggatatct catgtaactt aggctcgaca aatatcgtga atttgatgca
     9301 aagccctaac tttggtcaat ctgtgcgtac gatgactcgt gcattgacat ttattacaga
     9361 tgcatctgat atcgatgtcg taccaccgat ccaaaacgga aataagctga atcatacgat
     9421 tggattaggt gccatgggct tacacactta tcttgcaaaa gaacaaatgg aatatggctc
     9481 agaggattca ttagacttta caaatattta cttcatgctg ttgaattatt ggacacttgt
     9541 tgaaagcaac aatatcgctc gtgaaagaaa acaagttttc cataattttg aaaaatcagc
     9601 ttatgctgac ggcacttatt tcgaaaaata cgtaactgga gaattccagc caaaatcaga
     9661 aaaagtcaaa gcattgtttg aaggcatttt tatcccaact gctgaagatt ggaatgcttt
     9721 gaaacaagca gtaatgaaag atggactgta tcatcaaaat cgcttggcag tagcaccaaa
     9781 tggctcaatt tcatatatca acgatacgag tgcaagtatt catcctatca ctcgcatgat
     9841 cgaagaacgc caagagaaga aaatcggtaa aatttattac ccagcgcctt atctatccaa
     9901 tgagacgatt ccgtattaca cttctgctta tgatatggac atgcgtaaag taatcgatgt
     9961 ttatgcaatg gcacaacaac acgtcgatca aggcatgagt ttgactttat tcatgcgttc
    10021 tgaaatccca gaaggcattt atgaatggaa gacaacaacg aagcaaacga cacgtgattt
    10081 gaatatttta agacattacg ctttccacaa aggaatcaaa tccatttatt acgtacgtac
    10141 ctttactgat gatgcagaag aaatcggaag caatcaatgt gaaagctgcg tgatctaata
    10201 tcatagatag attcgcttat tcatatttta attaaaggag cattctcatg tcagaaacat
    10261 attatgaagc aataaactgg aatgaaattg aagatatcat tgataaatcc acttgggaga
    10321 agctgacaga gcaattttgg ctagatacac gtatcccact ttcaaatgac ctagatgatt
    10381 ggcgtacatt gtctcaattg gaaaaagata cagtaggaca tgtatttggc ggtttgacac
    10441 tgctagatac tgtccaatct gaaagtggca tggatcagtt gcgtaaagat gtccgcactc
    10501 cccatgaaga agccgttttg aacaatattc aattcatgga atccgttcat gccaaaagtt
    10561 attcttctat ttttagtaca ctgaatacga aaaaagaaat cgaagaaatt tttgactgga
    10621 caaatacgaa tccttatctg caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg
    10681 gtacaccgct agaaaagaaa attgccagcg tctttttaga aacattcttg ttctattcag
    10741 gtttttatac tcctctttat tatttaggaa acaacaaatt ggctaacgta gcagagatca
    10801 tcaaactaat catccgagat gaatctgtac atggaaccta catcggttac aaattccaat
    10861 taggattcaa cgagttacct gaagcagaac aagaaacgtt aaaagactgg atgtacaatc
    10921 ttctttatga gctttacgaa aacgaagaac gttatacaga agaattatac gatccaatcg
    10981 gctggacaga agaagtcaag acattcctac gctataatgc caacaaagca ttgatgaacc
    11041 tagggatgga tccactcttt ccagatactg ccaatgatgt caacccaatc gtaatgaacg
    11101 ggatttcaac aggcacaagc aaccatgact tcttctcaca agtcggaaac ggctatttgt
    11161 taggtcatgt agaagcgatg aaagacgacg attatttgat tggattggaa taaggattta
    11221 ttttaattca aaaatttttc gaaaaataaa aagagtttag cgtacagata ttttaaatgt
    11281 acactgaact cttttttatg cttattcact tgtttcttcc agcacttttg gactgtctaa
    11341 cttttccgaa acatgttcca ttcttctcaa taaatagatt ttccggatat ttaatattat
    11401 cgtcttaatc actgcataga caggtatgca aatcagcatg cctaatattc ctgcgacact
    11461 tcctgctcca atcaatacca agatgatcgt caaaggatgg atattcattg atttcccaaa
    11521 taacaacggc ttgatcaaat ttccatctaa ttgttggatc actaaaatcg acaacgccat
    11581 atacaaagct tgaaatggag agataaacaa accgacaatg attgctggta ccgctccaat
    11641 aaaagggccg acatacggga taatattcgt gattccgcaa atgaccgcaa gcaacaagct
    11701 ataaggctga tcgaatattc tcattgccaa aaagctcatt acaccaataa tcaaagcatc
    11761 taagatcgta ctgctgatat aggcagataa tgtttcgttt aattctttta ccgtttggcg
    11821 taactcttta cgaatcgctt tcggaaagaa gtgagacaac gcatcaataa atttgtgccc
    11881 atccttgaac ataaatagca aaataaatgg aacggtaaat aacaaaataa aaaattgcat
    11941 cataaatgaa atgatttttg agacacttgc agataaactg actaatatga tattgaacaa
    12001 attactaaga gataaatttg ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc
    12061 ttcccgctgc gccaattcat ttaaccattt gcttgtttca tctgcataca ttggcaagct
    12121 ctgggtcaat tgaattgtct gttccattaa ctgaggcact acattcatga cagttaaaac
    12181 aactaaaatg attacgacca aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg
    12241 cttttcgaga aaaacaacga ttggatcgaa catataatag ataaaaccag caacaagcaa
    12301 tggtaaaaaa actgccgaaa gcattttcaa tattggacta aaaatattcg gcatttgtaa
    12361 aataaagtac acgccgatga tcgtgagtac tagctccact gtccagaaaa ataattttga
    12421 ttgtttaaaa cgtgattgca tacatctcct ccaaggtcct ttgatacttc tattctatca
    12481 tttaacaaag agttacgcgc tgagattttc atttgcttaa aaaaaacaaa aaaaataaac
    12541 atattttaat gagtagacga gtacttaaac aaaaacacgg tttggagtag tgacaaaaat
    12601 tatactgcag tccctttttc caaataaacg gtgttcaaaa gcttgcatct tcgtaataag
    12661 cccagccaaa cgaaaaatat aaggaactat ttttatttgg ctgggcttat tacttgatgt
    12721 aggacatcta ttttacaacc tcagatcatt tattgcgcaa tactgactaa atgcaacttt
    12781 gtgatatttt tgtgtacgga caactcctcg atcacatcgg cataagtgag cccttttgga
    12841 agatcaatcg tatagatatt tttgtacact cggtagtcat caacgaattt tacgtcaaaa
    12901 ttcacatctt cgattacgat atttttctct tcaaaataat cggtaagaaa atctttggtt
    12961 tcttttctat ggataaattg gatttctaac tttttagtcg taggtacatg gatcacgcgt
    13021 ttcactactg tgagtgcaat agcaatcccg ataaaactga tgatcgcaat ggaatagtaa
    13081 cccatgccga tggcgatccc taatccagca attgcccata aagaagcggc agtcgtaagt
    13141 cccgtcaccg attgtttcgt gacgatgatc gttcccgcgc ctaagaaacc gacaccactc
    13201 accacttgag caatcagcct tgcttcatct gaacgaatcg ttccagcaag ctgtggatac
    13261 tctaaagcat ctcttaatgc accactagcg atctcaactt ggatcaatgc aatgatacat
    13321 gctcccatac aaactaaaat atgcgtacgc atacctgctg gacgattttt atattgtcgt
    13381 tcaaaaccga ttgctccgcc aaatagcata gcgatcccta aacgtaaaat aatctctgga
    13441 attgtcaata ccatatcatt catctaaaca ccctccagtc caatatatca tgtctgaaag
    13501 gagcagacaa aaaaagtgca atttttacct cttacgaaaa aaggactgtg acaaaatttt
    13561 tgtcatagtc ctttttatga ataaacggtg ttcaatcagc tgaccctcgg tattactcaa
    13621 caaacggcaa aatatgggga gctatttttc ctttgtcttc tctaatactc aggtcaaaac
    13681 gctgatttca caacctcttt agcctactat tgaaaattac aacttacttg ctgcttcttc
    13741 atcgatgatc acaacaacat tgtcgtgatt ttgcaataca cttgctggca aatgattcgt
    13801 tacaggtcct tcaatcatcc ctttgattgc atcagctttg ctttcgccaa atgccatcaa
    13861 gacgatttct ttacttttca taattgaacc gattcccatt gaaacagctt ttgttggtac
    13921 atcttctgct ttatcgaaga atcgtttgtt tgcattgatt gttgattctg ttaagtcaac
    13981 tactgaagtc gttccgtcaa atgaagcacc aggttcattg aaaccgatat gtccattttg
    14041 cccgattcct aagatttgga tatcgattgg atgtgcatcg atgatttgat catacttttt
    14101 gcatgctgct tccagatctt ctgcttttcc atctggtaca aacgtttctt tgaatggttt
    14161 tttatcaaat aattggacat ccatgaaatg acggtagctt tgttcatctg aaccgcctag
    14221 tccaacgtac tcatctaagt ttacagaagt catttcactg aaatccaaat cgcttgaagt
    14281 catttcttta tataaagtaa ctggtgtgct tcctgttgcc aatcctaata cttttgcacc
    14341 tttgttcata ccttctttga tgatttcaaa tgcttttttt ccgccttctt cagcgttttt
    14401 tacacgaatg atttccatag tgattgctcc tttatatttt ggtatagccc aattatagca
    14461 ttcttttttt ctttttacaa taattagtat agaccaaatt gcattttttt cattttcttg
    14521 acaataaata ggaatccgtg ttaggtttca taagatggtt caatcaaaca atccatgttg
    14581 catgaaaacc aagcaagtga ttcactgtgg acgctttatg tctgcagttt ttttatgaaa
    14641 aaggagttat tatgagtata ctgacattaa acaacattac ccagcaattt ggcgacaaaa
    14701 tcctctatga aggtgtcgat ctgcaattaa atgctggcga acatcttggg ctgattggac
    14761 aaaacggagc cggtaaaagt acattgatta aaatcatcac tggtgagatc ttaccagatg
    14821 acggacagat caattggcaa aaaaatattc acaaaggtta cctcgatcaa tatgtggaag
    14881 tcgatgaaac attaacaatc gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa
    14941 aggaagaaaa aattgctttt ctttaccaaa catatagcga gaccttcgat gattccttgc
    15001 tggaaaaagc tgggaaactc caaacggaat tagaccaagg agtgttttat caaatcgaca
    15061 ccttagttgc tgagatgagc agcgggttgg ggatcgatgt cttaggttta gattcactgt
    15121 tgaaaaatct aagtggtgga caacgttcaa aggtgatttt agcaaaacta cttttagaaa
    15181 acccagatgt tctgatctta gatgaaccaa ccaatcactt agatgatcag catatccaat
    15241 ggctgacgca gtttcttcag gattttgacg gtgcgtatat cgtgatttcc catgaccaag
    15301 aatttcttga tcagatcacc acccatatcg cagacatcga gtttggaaag attactaagt
    15361 atactggcca tctaaaacaa gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc
    15421 gccaatatca tgctcaacaa aaacaaatcg aaaagaccga agcttatatt cgaaaataca
    15481 aagccggttc ccgatcaacg atggcaaaaa gccgacaaaa acaattagat aaaatcgaac
    15541 ggctgacgcc tccagcttca gcaagtaaag ctgtctttga ttttccattc tcgcctattg
    15601 tcacgacatt agcagtggaa acaactgatt tagtcatcgg atatgatcga ccgctacttg
    15661 cacccatcaa tttgaccatc cgctttggtg aaaatattgc cattcgaggc ttcaacggga
    15721 ttggaaaatc tactttatta aaaacactga tcggtgagat tgaaaaaata agtggcgatt
    15781 tccacttccc agataacaca aaaatcaatt acttttcaca agatctgtac tgggaaaatc
    15841 cattagaaac accactacaa tatttaagca gtcaatttcc aaaagcgact atcaaagaat
    15901 tacgtcgcca actagctaaa gcagggttag ttaatcaatt agcaagtgaa cctctcacta
    15961 cattaagtgg tggggaacaa actaaggtga aacttgcaca gttgacaatg aaccaaggta
    16021 atttactgat tctcgatgaa ccgacgaacc atatcgatca agaaacgaaa gaaagcttac
    16081 aagacgggat acagaaattt ccaggaacag taatcatcgt ctctcatgaa caagaatttt
    16141 atcaagatct tgtgcaacga gtcattgaga ttgaaccaaa ataaacgttt gattgaatag
    16201 ttgaaaaaaa ggagctgtga caaaagtttt gtcacaactc cttcttctga ataaatggtg
    16261 gccaattagc tgaccttcgg tattagctca aaaaacggca aaatatgagg agctattttt
    16321 atttggctat tcttattact tgatgcagaa cagcttttca caacctcttt tcgtctattt
    16381 tttattttcg agatttctct ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc
    16441 ttgaaaaaca agcccgctaa ccgttacacc taaccagcca tactgctgcc acattgtcgt
    16501 ccctagcaat gaaccagccg cacctccaat aaagtacata aacataaata cagtattatt
    16561 tcggttgctt gctgcttcac ctagcccttg tactcgtgtt tgattggcga cttgaccaaa
    16621 ttgattcccg acgtctaata aaataatagc taaaagcaaa atggccaaat ggcttccgcc
    16681 aaccaataaa gcaataaagc ttattgtttg cataacaagt cccatataca cgatttttct
    16741 ttcagaataa gaatctgcca gtcgtcccac gataggtgca gcaactgctc cagacaaacc
    16801 aaaaatcgct aatatccctg cttcgaatgt tccccaattg taggcgggac tgctgatata
    16861 aaaaatcaac gtagaccaaa agatcgaaaa tgttccaaac atgaaaaatc cactgactgc
    16921 cgcttctctt aatagacgtt gagaagcaac taaatgcggc aaacttttaa gtgaatcaag
    16981 gtatgaaaca gctgtactac tttgctggac agaacgcggc attttccagt aaagcagaag
    17041 tgtaagaatt ccgactgcac ataaagcaaa taaatagatc atccgccaag aaaaaagact
    17101 ggcaacaata ccagataccg tccgagaaag caaaatcccc acgagtaaac cgctaagcaa
    17161 tgtacccatg accttccctc ttgcttttgg tcccgctagt accgcaccgt aaggaatgat
    17221 gatttgcggc acgatcgaaa gtaatcctat taaaaaagaa gcgcatgcaa ataacgtaaa
    17281 cgaaggcgca aaaaaagcgg ccaacagaga gacggcagat aacgctgcca ttcgaatgat
    17341 caattttgga cgatccacca cgtcccctaa aggaactaaa aacagtaacc ctaacgcata
    17401 gcctaattgg gtcaacatcg tcaatatccc gactgcactt gtgctaacag ataaactagc
    17461 tgctactttt gttccaatcg gctggatgta atacatattg gcaacaacaa cgccacaagt
    17521 gattgataat agtaaaataa gcgaagttgt taaaggtttt ttttcattac tcttcattga
    17581 tttcccccat ttcctatctt agtttacaac ttatggaaaa gattggaaat agaaaaatac
    17641 aactattcgc cacacttatt atttgtgctt ttgttttcct tgttttttct tgcgatcttt
    17701 ataaatgaag acaactaacc ctactagcac agtaacactt gcaaccaacg tgactgcagt
    17761 tttgttatat ccgtgaaaca gagcttctcc accacacgca tacaacacag acgaagggat
    17821 gactccaatc acgatcgaca atatgatttg ctgtagaggc aatttcatcg catctgcagc
    17881 aaaattgacc atagaagatg gaataaccgg tatcatatag cctaacatta cacctatttt
    17941 tggatgtttc atttgtcgga ttgcttgcac ccaatgattc gtttcggttt tcttatctaa
    18001 aaattttaag cgatgcataa ggaaaattgc agtgatgtta ccaagagcat tacccaaaat
    18061 gttgatgatc gcacctgtca gtggtccaaa gctcaaacca gcaatgactc caactactga
    18121 agtaggaata cctggaacag cacacatcac cgctgtcaat acgatcaaga gtatcgatgc
    18181 aaaagtccca tgcgaacgaa tatgtgcgag tagctctgcc ttactcgctc tcggatttaa
    18241 aaataattgg atgtctgtct gatattctaa atacaaccga tagcccacta ccagtatcag
    18301 aatgattccc aacgagatca atatcaatcg gattattttc tttttcatat ttcctcctga
    18361 aaaaaatcta tattcattaa ataatagttt aacataaaaa caaaaaagca ccgaaatcca
    18421 acataggatc tcagtgcttt taaatagtaa aaattattta acagttacag atgcgccaac
    18481 ttcttcaagt ttagctttca attcttcagc ttcttcttta gatacgcctt ctttaactgg
    18541 tgcaggagcg ccatcaacta cagctttagc ttctttcaag cctaaaccag ttgcttcacg
    18601 aactgctttg ataactttaa ctttttggtc tccagctgca gttaattcta cagtgaattc
    18661 tgtttgttct tcagcagcag cagcgccacc agcagcagct acagctacag gagcagcagc
    18721 agatacgcca aattcttctt caatagcttt aactaggtcg ttaagttcta ggatagttgc
    18781 ttcttttaac tcagcaacga tgttttcaat gttcaatgcc attataattt cctccgttta
    18841 attgttcttt tttatttaat gctttatgcg gcaattaagc tgcatctcca tcttctttgt
    18901 ctgccactgc tttgacagcg taagccacgt tgcggactgg cgcttgtagt acagatagca
    18961 acatagatag aagtccttcg cgatttggca attttgccaa tgctgtgatt tcttccaatg
    19021 aagagacttt accttcgatg ataccgcctt tgatttctaa tgcttttgct tctttagcaa
    19081 attcgtccat gattttcgct ggtgcgacta catcttcgtt actgaatgca acagctgtag
    19141 gtccagtgaa tacttcgtcc atgccttcaa ggccagcttt tttagctgca cgtgaaagaa
    19201 tagaattttt gataactttc atttctacgt tagcatcacg aagttgttta cgtaaacgtg
    19261 ttacttcatc aactgttaat ccgcggtagt caacgactac tactgatgca gctgctgtaa
    19321 atttttcgct tacttcatca acgattgctg ctttttttgc gataattgct tcactcattt
    19381 ttgagtttca cctcctgaat tttgatggaa gagttttttg cactgaaaag gcaagtcact
    19441 tttatagtta ctttgctcaa ccagtattat tcatcaccta ttcacttcgt tcatagtgat
    19501 tctactcgct ttgctcgaaa ggaaactcct ttcgctttgc tcaaccagta ttgttcatca
    19561 tctcttcact tcgttcatag tgattcacaa taaaaaactc tatgccaccg cagacataga
    19621 gggactattg aatatattca ataaacgtcc tcggtaggaa attaaggctt gatgccacct
    19681 actgtcttcg gtacaagtta attattaacc tcaactagct tagcatgcta aactagttga
    19741 gtcaagaatt atttttcagt tggatcaatc aacattctaa tatttaatta gaaagaagct
    19801 tggtcaacat ggataccagg tccaaatgtt gttgtgatcg ttacattttt catgtattga
    19861 cctttagctg ctgatggttt agcttttagc aatacatcat ggattgctgc aaagttttca
    19921 actaattttt catcgtcaaa tgaaactttt ccgattggta catggatatt tccagctttg
    19981 tccacacggt aagttacttt accagctttc acttcgttta ctgcttttgt tacgtccatt
    20041 gtaactgtac cagttttagg gtttggcatt aagccttttg gtcctaatac tcgtcctaga
    20101 cgacctacag tagccatcat atcaggtgtt gcaacaacta catcaaagtc aaaccatccg
    20161 ttttggattt tttgaaccat gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct
    20221 tcttttgctt tgtctccttt agcaaaaact aaaacagttt gtgttttacc agttccgttt
    20281 ggtaatacaa cagcaccacg gatttgttgg tctgcttttt tagggtctac gttcaaacgg
    20341 tatgcaactt caactgttgc atcaaatttt gcaatgtttg tttcttttgc taaagctact
    20401 gcttcggcaa caggataaac tttacttgaa tcaacttttt ttaatgcatc ttgcattttt
    20461 ttgctctttt tagccatttt ggttcctcct tgattgtggt tataacggtt gtacctccca
    20521 cgtgttctat atctttcaat atagagccaa ctgagaagct gctgtcttag ggtgtataga
    20581 ttattctaca gtgatcccca tgcttcgtgc agttccttca accatgcgca ttgctgcttc
    20641 aacgttagct gcgtttaggt cttccatttt taattcagcg atttctctta cttgatcgct
    20701 agaaactttc gcaactttat ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc
    20761 ttttttcaat agaacagcag ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc
    20821 gtatacagaa ataacaaccg gaatgatcaa acctgcttga tcagctgtac gagcgttgaa
    20881 ttcttttgtg aatcccatga tattgatacc cgcttgacct agtgcaggac ctactggtgg
    20941 agctggagtt gctttacctg caggaatttg caatttaacg attttttcta ctttctttgc
    21001 cacgagacat acctccttaa gtccgtgatg tggttaattg gagatgcata tttctcctcc
    21061 cacgttttat ctgatctgaa aaaatccaga caaataatgt gcaaaacaat gcacactggt
    21121 acagtatagc aaatttattt gttttttcaa gatttttttc tgctattttt acattttgct
    21181 tgaataaacg ctaaatttat tctaaaaaaa caacaatttt tcgttctcat ttcttctttt
    21241 atgttacact tgcgctagca tggttcaaca gatgaaagca aacaataaca cggtttttta
    21301 gacccttgta caaagattcc tacataacaa aatgaggtgg agaaaatgaa agatttaagg
    21361 tatagaagtg tctttgatat tattggacct gtgatgattg gtccaagtag ctctcacact
    21421 gccggagctg ctagaatcgg gaaaatcata cgtagtatat ttggcgaaca gcctgattct
    21481 gtagatattt atctatatga atcatttgca aaaacctatc gggggcacgg aacagatatt
    21541 gcattagttg gcggcctgtt agggatggaa cccgatgatg aacgtttagc tgattctttg
    21601 aaattggcat atgaagccgg tatggaagtc ttgtttgttc ctaagagtga aaaagccgaa
    21661 catccaaact ctgtaaagat gcttctaaaa aaaggaacac gaaagctttc agtcactggc
    21721 atttcgatcg gcggtggcaa tatccagatt tctgaattga atggattcaa aatttcttta
    21781 agcatgggta ctcctacctt gatcgttgtc caccaagatg ttccaggtat gattgctaaa
    21841 gtgactagta tactttctga gaccaatacg aatatcggca ctatgacagt gacacgggaa
    21901 tcaaaaggtg aaaatgccat tatgataatt gaagtagatg atcctcaagt agaagaaacg
    21961 gtaaaaagac tcaaacagct gccaaatatc gacagcgtaa attatttcga ttaaggagct
    22021 aaaaaaatgt ttttatcgat tgaagaatta gtagatcaag caaaagctta tccaagtgta
    22081 gcagaattga tgattgcagt tgaaatggaa atgagtggcc gttcccgtag tcacatcatc
    22141 gagatcatgg aaaaaaacct ctcagtaatg gaacaatcaa tcaacgaggg aaaagaaggt
    22201 gttacctccg tcacaggaat taccggtggt gatgcaaaaa aattagatga ttatttaaat
    22261 gaggcagact ttttaagtgg agaaacaatc ttaaaagctg tacgcaatgc tgtagctgtt
    22321 aatgaagtca atgcaaaaat ggggttgatt tgtgctacac cgactgccgg aagcgcgggt
    22381 gttgttcctg gtgtgctgat ggctgtccga gatcggcttg atttgacaca tgaacagcaa
    22441 ttggaattct tatttaccgc tggcgctttc gggttagtca tcgcaaacaa cgcttctatc
    22501 agtggtgcag aagggggatg tcaagcagaa gttggttctg caagtgcaat ggcagcagca
    22561 gctttggttt gtgcaaaaaa tgggactcct catcaagcag cacaagcagt agccattaca
    22621 ttgaaaaata tgatgggact gatttgcgat cccgttgcag gcttagttga agtcccgtgt
    22681 gtcaaacgta atgctttagg ctcttctcaa gcttttatct ctgctgatat ggcattagcg
    22741 ggtatcgaaa gtgtcattcc gccggatgaa gtcgttgctg caatgtacca agtcggacgt
    22801 caaatgccgt cgattttcaa agaaacagca gaaggtggct tagctgttac gccaactgct
    22861 aaacgacttg cggaagagat tttaaataag agggcgtgat aaagctcctt aatttcgagt
    22921 attaagagat tagttatctt cataacaaaa aacgaagaga ggactaattg cccttctctt
    22981 cgttttttat taatccatca atttcgtatt ttttacttca tcgatcgttt ttgttcctgc
    23041 aagttgcatc gtgatcgaca attctttgtt caggtgttca aaaacagatg ttactccttc
    23101 tgccccacct agattcaacc cataaatcac tggtcggcca atcgcaacta agtcagcacc
    23161 acttgccaga gctttgaaga catgctctcc tcgacggaca ccagagtcga aaatcactgg
    23221 tacacgtttg ttgactactt cggcaatttt tggcaatact tcaaaggacg caggacctcc
    23281 atctagttga cgtccaccat gattggatac ccagatacca tctgcccctg cagagatagc
    23341 gattactgca tcttctggcg attgtattcc ttttacgata actggaagat gggtgatttc
    23401 tttgattgtt ttgatgtcat ctggtgtcaa gccttgtttt gcagctgcat aaatttctgc
    23461 gattcctttt ccttcgccat taccacttgc actttgttcg ctatacgcag ctaagttagg
    23521 cattggtaat ggaaattgga attgattgat gacgtcttct tcacggtaac caccgagcgt
    23581 tgaatctgcc gttaaaatga tagctttagc ccctgcttct actgctttgt tcaaaatgaa
    23641 ctcattgaat ctgtcgtctt tactcatata taattggaaa aactgcggtg cgcctggcgc
    23701 tgcatcggct gcgtctttga ttgtcgtatt tgcataagtg ctgatggaaa agattgatcc
    23761 agcagcagct actcctttag ctgtatcagc ttcccctttt tcatgtgcca atccttgggc
    23821 cgctgatggc gcttggataa tcggtgtatc taattcaatt ccgaaaacag atgtatgcaa
    23881 atcggctgag tcgattccgc gtaatatccg tggcatgatt ttctttgtat taaaagaagt
    23941 cgtattttct ttcatcgtcc actcatcttc agaaccaccg cgaatatagc caaaagctcc
    24001 tgcttccatg cgctctttta cacgaccttc tagttaggca atgtttacaa tatcgattgg
    24061 atgttcttca ttacttgtat gataagtcat agtagatcct tctttcatct tcttgattac
    24121 atataaatca tactaaactt acaaaaagta agcaaacatt tactctcaga tacttttatc
    24181 gctattcttt ccaaataaat cgacttacaa gctgatctac ttcttggttt tcatgaagca
    24241 tactatgaga ggcattttca cctgtcacta ctttttcctc tatttcattt ccgcgttgct
    24301 tcaacaacgc atagaccgct aatgcactgt ttaacggtac tgttccatca ctcaagtctg
    24361 tttcatctag ttgaccagca atcaagaaaa atcgagtggt tataggaata ttaccgatca
    24421 tttgctggta atcttgatag cggttagaaa cgacagcagg cccctttgct aactcatctg
    24481 tcagagactg cgcactatca tcgacaaaat cattaaaagg cgcgccaatt gctgttaatg
    24541 tctgaacttg tggcaatgac gtatcctgtc catacgttcc taaatagcgt agtgcactaa
    24601 ctcctcccat ggaatgcccc actatgttga ctttatctat gccgtagttt tctttcagat
    24661 acaaaagaca agttttgatc cattctgctt gattccactc gttattttta ttatcttcaa
    24721 acaatacttg gaccatcgga ttgtctgctt ttccagacaa atctcctgtt gcttgtattt
    24781 gtccatctgt tcctactgtt aaaaccaatt cttttactgc ttgctgattt ttttccaaac
    24841 gccgcaacat ccctttaaag gaattttctg tcccgctgta gccgtggaaa aaaagtgttg
    24901 gaatagtggt tttttctatc actttactgg attctgccgt tttttcttta tcagaaactt
    24961 cagtagtatt tatcttcgtc gtctggcaac cacttaacgc aaacaaactg ataaggacta
    25021 aagctgctgc agttgcacgt ttgcataaat ttttgattgg ttctgtcata cacctcttcc
    25081 tttccatata gaaaaaagaa agacttgaaa ggccaatcat atgatcgaca tttcaagtct
    25141 ctttattaat cgattttatc tacttgatcg aaatcaagtt ctgtactggt ttcacgtcca
    25201 aacatatcga tgttgacttt caatttttct ttttctccgt caacttctgt tacttgtcct
    25261 tctaatccag caaatgcgcc ttcgatgatt ttcacgactt cgccaacttc tacttctaga
    25321 tcattttgtc gcaggctcat accgatagaa cgcaagatat gattgacttc ttctggaagt
    25381 aatggtgctg gtttgcttcc ggctccgtgt gaaccaacga atcctgtcac accaggtgtg
    25441 ttgcggacaa cataccatga agcgtctgtc atgatcattt caacaagtac gtatcctggg
    25501 aatgttttat gaacgatttc tttttctttc ccatttttga cttcagtttc tttttcttca
    25561 ggaacaacga cacggaaaat aaagtcggcc attttcatac tttgcgcacg agattcgata
    25621 tttgctttta ctttgttttc atatcctgaa tatgtatgca agacatacca gtttttttca
    25681 aaagattcca ttgaaggctc ctcatttcat tgtaaaataa aaaaacctca gtacacccga
    25741 agtttttctt tcgcttcatt atagcactaa aagtacgttg tggctagcga gttattttaa
    25801 aatccagcca aataagcttt ggatggctgt atccattacg taaaacatca atccgaaaat
    25861 aatagaagtt tcgatgacta ctaacgtatc tttacggagt tgttttctgc ttggccaagt
    25921 tacttgtttg atttcatctt ttacgctttt tagaaatttc atgagacaac ctcccttgct
    25981 actgtttatt tcgtttcttt atgtaatgta tattgattac aatatttaca aaacttattg
    26041 atttccaaac gttcgccgct ttttccttca ctgactgatt ttgtgtagtt gcgagaccca
    26101 caaacggaac aagcgagtgc tgcttttttc tttgccattt acttctgccc ccttgctgag
    26161 aatcagactt atacttcata acagtatcac gtaacttttt acttgtcaat ttgtgaaata
    26221 aactatctat tgttttggat aaaattatga tatgattaat ttgaaattaa gctaaaatga
    26281 gacaattgga ggtatagcga tgctattaaa aacacttgta tataagaaaa aagatctgac
    26341 aaccgttaca gagactagta cattagaaga agcattaaaa attttagaaa cttccggata
    26401 ccgttgtgtg ccgatcttag attcttcagg taatattttc cgtggaaaca tttacaaaat
    26461 gcatatctat cgtcataaag caaatggcgg tgatatgacg cttcctgtca ctcatctttt
    26521 gaaaaacgct acaaagttca tttacctgga tacttctttc ttcaaagtat tctttacgat
    26581 caaagagctt ccttatattg ctgtccttga cagtgaaaat catttttatg gtattttgac
    26641 acatagtacg ttgcttaata tgctctctca atcttggagc gtagaacaag gaagctatgt
    26701 actgacaatt gcttccacag gaaagcaagg agatttggct gctattgcaa aaatcatttc
    26761 aaaacacagc agcattgcca gctgtattac tcttgatatc gaaaaagacg aatacattcg
    26821 acgtacattg atcacgctac cggctaacac tgaagcgaaa gtatgtgaaa cgatcgtcaa
    26881 ccacttagag aaaaagaatt ataaagtggt agaaattgaa aatttgcaaa accaagaagc
    26941 ataatcaaaa agcgaaaaaa gaaaacagct aatctgcttt cttttttcgc tttttataat
    27001 tagacacgct tttcaaaatc aagcgcgcga tctaaataat acatcaatgg tgctgaaaca
    27061 gccatgacga taaactcact taatgcaagc gtgccatagg ttgtccaaaa tgcttggcca
    27121 cctgttggaa ctaacatata agcaatcagt cccatgctga tggtaaagaa gatcgtgttc
    27181 aatactaaac gcatctttac atttttcact ttgttttgca ataaagctgt caggcctaaa
    27241 gaaagcaatg tttggccgcc accaaaaacc acatccatga taccaaaacc aaagaaggca
    27301 ttatagacga tgaccccgcc taaaactccc caaagcaatt tacggttaaa gacaactaaa
    27361 tgattcaaac tttctgaaat acgaaactgg atcggccctg aggatacagg cgcaactaaa
    27421 atagttaacg ctagataaag agccatgatg atgccattta caacgattac ggataaccgt
    27481 tgtctgcttt tcgttttttg cattttctct cctcttttct cctagttttt ttaacgcggg
    27541 atggttgatg aaccgcggtt atgtgaaaca aacaataatt ataacagact tttcatttat
    27601 ttcaatccgt cttatctgct tttaaaaaag caaaagctta aacaatgtaa acgtttgcta
    27661 aaaatcgtgg gatatggtat aaagtaagtg taaggggtca taagaattcc cagtaaagca
    27721 gacgaagtag gatctgcaag aacagaattg ttgtgaaact taaataaata aaaaaggatg
    27781 tgggaatttg agcaacctct tactagagaa cggtctagca agcagctgaa gatctaagtg
    27841 gacatgccga accttggaac ttctccttac gtaagtaatg gatcatgcgc ttgctaggct
    27901 tttttgtgtt tttcggtctt cggacctttt tcattttcct agacttctgc tataataacc
    27961 aagaattaat tttgtaacag atttttttga aaaaacaagt ttctttcatt caaaaaatag
    28021 aaaaagcttg ctaattattg gttaaccagc aaacaggagt attaaaatat cttgttttat
    28081 tgaatagcag gaggaaatca tgaaattcca ttatcatata ttgatcaatc catcggcagg
    28141 cagcggaaat ggctataaag tagcagaaag aattttaccg gtattaaaaa ataagcatat
    28201 tgactataca cttcattata gtgaatacaa aggacacgat gctgagattg ctgaaacttt
    28261 ggcaaaagaa acattgattc cttggataga agaagaacag aaaacaatcg atactttccc
    28321 ccttttgatc atcgtaggag gtgacggcac cttacatcag gtacttgata ctttctatca
    28381 gatggaagta gaattcccag ttgcttatat tccagcaggt tctggagatg attttgctcg
    28441 aggggcaggc cttccaaaaa atcccaaaaa aggacttcag ctgattttag cagctcaatc
    28501 accagaaaaa gtccacataa tggcttatga agaaaaaata agtgaaaaaa aaggaatagc
    28561 tgttaataat ttcggtatag gattagatgc agctatcgtc catgcgacaa atcattcttc
    28621 cacaaaaaag cgattgaaca aatacaactt aggatcattt tcgtatttat tctctattct
    28681 ccgtgcttta ttcactcaaa aaggatttcc aattctggta gatgcaggag gaaaacaatt
    28741 aagtttttca aacgcatttt tatgtacagc aacgaagcat cctttttttg gcggaggaat
    28801 cgcgattgtt ccaactgctg atgcgtctaa accagtcatt gattttgtcg tggtcgaacg
    28861 aatcaatttc tttaaaatct tgtggctcat tcttttactt atacagaaaa aacaattgaa
    28921 atcgaaatat ttccatcact acacaacaaa cagattacgt atcgtttcaa caacaccgca
    28981 acatggtcaa gaagatggag aagaaatgga aaaacgacct tttgatatca caataaccac
    29041 aactaaacag ttgttttggt gttaataaaa gaggttttgt cagaagctac atctggaaca
    29101 ccgtttattc ggaaaaaagg actgtgacac gacttttgtc acagtccttt ttttactatg
    29161 cagttaattc tttgcccaat tgggttgcca tcgatagttt ctttctttac ccaaaataaa
    29221 ttggatcatt gaaaacaccg cagcaaaagc aactaagaca caaccattga accaaaaaga
    29281 ttctgcaaat gtttgattgt aactgagtgt ttcactgatc aagttattat tttgaacaaa
    29341 ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt tctggcaagt attctgctgg
    29401 tacaaacacc ccaccgataa agcaactgcc tgtgataaaa atatctttga tcccaccgct
    29461 ggtttctgag ttttttacca atgaagtgat acaagccgaa aagctaatga tcggcaataa
    29521 aaacaagaaa gtatttaaaa tatgatacca taccgttata tcccacgagg tgtggatcac
    29581 taacagaacc attagaagta agaagccact gaacaacagc agcgagtaac taatcaaact
    29641 aaaggttact ttgcgattca agtttcgttt ggtaatgggc gcactctggt ttcgtttact
    29701 gatctctttg cgattaaatg ctagattgat gacgctgaaa ccgctaaaaa tggatagaaa
    29761 caacccataa gacaagaaat taaaggttgt gcccgtcaac agctttttca tccgctgact
    29821 atatcccgca cttagctcaa cttctccagt aaccgagagt gccatttgtg tctgttccat
    29881 agctgcatcg attgttttat cttcttcttg aaacagaatc agtgtttgca aaaaggtgtt
    29941 cacttgctgt gtaacaactg ttttagaaaa ggcatctggg cgactctgta tcttgatcgt
    30001 cggcatcttt cctacttcga cttgttcggt aaagtctgca ggcagatgta agataatgtt
    30061 tacacgatta aaataaagag catcatcgat ttctttttgt gaggttccat ccaatgtaac
    30121 gactgtttcc ttgtcattca aatatttacg aaggccatcg actacaggcg actcttcttc
    30181 actaatcaag gcgattttcg catcttgcag agaggtattt tcaacatcaa tattttgcgc
    30241 aaaaaagaat gtggcaacaa cagtgataat tacacctatc agtaaattcg atttatttgc
    30301 tttaagcaat gtccaaatcg ttttataaag cgtcataacg cacccctctt tccatccaag
    30361 catttcctaa cgcaaaaagg aaaataaatc cgatcaacca tagaagattg gtataaaaac
    30421 tagccaaatc agtgtagaaa taaagtttat acaaactttc gctgatcaag ttgacgagat
    30481 ttaattgacc aacaatcgga atatgaagat caatccaata cttcacttgt tccgacccca
    30541 tcataccagc aaaaaagctc atcgcaatcg tcactgaaat caagatgcta tccttttgca
    30601 ccagattcat ttttggtatc aaattaccaa ccaatgtacc taacaaaatc gcacacaacg
    30661 accccagagc ggtaacaatc atcaaccatt gccaacgttc accaaagtct acttgataga
    30721 taaaacgaaa gacagctaac ataatataaa tctctgtcaa aacaatcgta aagcttgctg
    30781 ccaaattagc gattgaaact aacgattgct ttctaggaat caaggacaag cgaataccat
    30841 ttgacgattg atttgcttgc tgatcctgta cattgcggac cccccacatc gtcccgtaga
    30901 gaatcgccat tccaatcaga gtgaagaaat aaaaactttt gaaactcata ttacgacctg
    30961 catgaacttc ttgaaaagat acttcttgtt caaaaacagt catgatgggc gcgagatcaa
    31021 cgttctccac ttctgccact ttactcgaaa ttataccaat acgctgtaaa aattgatcca
    31081 tgacgttttt catcaacgtc tgactcattc ctgcttgacc cacatggagc gaaacagatg
    31141 aaccatcaat gagataaaaa ccagccaaat catctgcatt tagcttttca ttcgcttcta
    31201 cttccgatag ttcctgtact tcaaataatg gtttatcatt gacttcgact tgttctaaga
    31261 ctgtggtaaa attttccgaa agcgctgttt gatccgcttt gattacaatt ccagtattaa
    31321 tcgtttcgag attgccacct tgatcaatct cgccaaaggc cgccataaac atcaatccta
    31381 aaagaattgg aaaggccaat gtccaaaaca acatggattt atttcgcagc agtatttttg
    31441 tgcgatattt ataaaaatgc caaaacatta tttcccctcc tctagtctct caaatccttg
    31501 ccagtaatct ctaaaaagac atcattcaag gacgcttctt tggttgctat ttttccgtaa
    31561 ctgatctttt cttcttgtaa gattgtaaga atcggcaaca atactgtatt tagttcagta
    31621 gttgttactt ggagctgacc ttttttataa gcgattccat cagaagctat tgttcgctga
    31681 caacacgttt tctgggagta atgggatgtc gatcgtcaaa cgatccgccg tccgtaccat
    31741 atcttttaga gattctttgg ttcctttagc aatcacctgt ccttgatcca tgatcacgat
    31801 ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt gtatagacaa tggttgctcc
    31861 ttgtcgattc agctccttga tactttctaa aattttattc cgactttggg ggtctacagc
    31921 tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg gcaatcccac atgcgatgtt
    31981 caaccgacgt ttcaagccac cactaagttc tcctggacga aacgaaacat atttttctaa
    32041 tcccactaat tgaatcacct cttcaatcaa ggctttcctt ttctcttttt cggaaacata
    32101 taaaccgcaa aaataatcga tattttcttg cacacttagt tcgtcaaaga ccgcaacgtc
    32161 ttgaggaacg accccaatca ttgccttgac agcataattt tcaggagtca ttgttgtccc
    32221 gaacaattta atggttcctt tgtcataatt taacaaagaa agcatacagt tgatggcagt
    32281 cgatttccca ctgccgtttg gtcctaacag acctaagact tctccttctt gtacttgcag
    32341 atcaaaatga ttcaatgcta caaaatctcc atagcgtttg actaatttat caatttcgat
    32401 aatcatcatg aaaaactcct tccaaatttc ttgttgatct gactataata aagtgcaaga
    32461 aaagaaacta gtgacgattg gctaaaccac acatgacatt tgtcacaatg aaggagtctg
    32521 ctatgcataa atctcggcta ctcaacgaaa ctagctttct actggttatc tcattgcttt
    32581 tgctggtaag tcaaggattc gttgctatgc aagttatcta tttattgatc gccgttttat
    32641 tcactggtgt cagttattta ctgcctacaa agccaaattg gattttccaa gttattctat
    32701 ttcttttatg tgccttttta cctgatcttg tcctgtttct gccagcgacg atgcgagtga
    32761 tcgcccctga agaaagccac cttgattact tgtttttgtt aggttttttg ttgatttcga
    32821 cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt aattttgctg tttttttcct
    32881 tttacttacg aaaaaaagat caacgaacga ccgatcttga gaaaaagtac attgtctcca
    32941 aagatactca ttgggaaaaa caacaagagt tatcggaaca aaataaagca ttggccctgt
    33001 cacaagaaac acaattagcc ttgcaaattg tggaagaacg gaaccgaatt gcccgagaca
    33061 tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt acaaattggt gcgcttgaaa
    33121 tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa tttaagtgcc accgttcaca
    33181 ctgggatgga tcggattcgt caaagtgtcc atgacctcca tgagacatcg atctcttttc
    33241 aacactcctt agagtttctg atcgctgatt ttccttcttc tgttacgatt gaaggaaact
    33301 tgtttgaagg attgaatgaa acgcagcaaa actgcttctt aatggtcatc aaagaagcct
    33361 taaccaacag tatcaaacat agtcatgcca aaaccgttgt tttacatttt cgaacgttgc
    33421 cggcctttta tcgcttgcaa atcacgaatg atggcaccag cccacaaaaa aattctgctc
    33481 ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga acaaaaggga caagtccata
    33541 ttttccaaga aaaccagcaa tttcagttaa cgatcatttt accaagggag ggacaccaat
    33601 gataacagta gtcattattg agatgatcca tttgtgacga aatcgttaca aaccatttta
    33661 gaaagcacga aagagatccg tgtgttaggc attggtcatt gtgccaagga tgcgctagtc
    33721 ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca tccgcatgcc taaacaaacg
    33781 ggcatcgatg ctgccaaaga aattttggct aaatttccca aagccatcct tttgcttttg
    33841 acgacattca aagatgaaga atacatccgc gaagcatttt ccatcggtgt caaagggtat
    33901 ttaatcaaac agaacttgca agcgattatt ccttctgtca aatccgctta taatggacag
    33961 gccgtatttg gtaatgaaat catcgagact ttcactcaaa tgatgaagaa taaaccgacg
    34021 attgatagtc atgcttatgc ttcttttagt gaacgtgagc ttgcaatcat caaagaagta
    34081 gctgccggga aaaacaataa agaaattgca gatgccttgt acttgagtga cggcactgtt
    34141 cgcaattaca tcagccaatt actagaaaag ctagatttac gggatcgcac gcaattggct
    34201 atctattttt atcagcattt gcaataagtc ttatctaaca caaaaaaacc tgaatgatgc
    34261 tccttcgcat cattcaggtt tttttcacat cttctattca attgtcccgc aaaccatttc
    34321 ctcacgattg aaagaatcaa ggattcgcag ggctgccagg attcgttgga ttgttcggcg
    34381 tgttaggcgg aaaaataggc gccttgtcta acgttttttc tagttttgat tcttcacgat
    34441 cgtctcgttc tacatcagga ctttcattca atttgaattc tttttccatc tcatctaact
    34501 ttttaaagtc attgccagat tcttttgcta atttcgctaa tttatcttcg gtttctttag
    34561 ccataaagta acgcccccct tttgatttca gcttagcata tcacactgaa aaaacaaaag
    34621 aaaacgctaa ccaacgatca tttttagcta tttctcatct ttaaaatcaa gcattttttg
    34681 ccggtataaa atcgtaggga gaactgtttt ccataccaat catcggatct actaactggt
    34741 tatcgatcac ttctgctgta agaacgaaat ctttagaaac agcaggttct acctcttctt
    34801 tcttttcttc tccaacaaat gaagaaaaca gatctggttc cactgtttcc attacttggt
    34861 ttttcgtttc attcgtccca ctgtcatctt ctacaggtag cttttctttt acaacccgtg
    34921 gctcttttgt atagttccca tcttcaaatg gatcatttcc gaccaaagaa tcttcataag
    34981 ctgctagttt ggtccggaga gtgatattca ttttatcatt atccatgatc cttgatttca
    35041 acgtagtcat ccggcttgct gattcattcg ctacacaagt gacataggct tgctcttccc
    35101 ctaacaaaat caacagttcc ttactgcgag tcactgccgt gtagagcaaa ttacgctgaa
    35161 gcatccgctg atattgatgg accatgggca aaataaccat cttgaattca ctgccttgtg
    35221 atttatggat tgagcaacaa taagaaagag tgattttatt ccattcgttt cttttataag
    35281 tgacctcatt tgcatcaaac tggataacta attcatccac tttatcttca gaatcttttg
    35341 ccaagatgat acccgtgatt tctcccatat ctccattgaa tacattcaat tcgggagtat
    35401 tgactaattg gaggactttg tctcctatcc gataaacggt gtcgttccaa tgtacttcct
    35461 tttttccagg tttccctgga ttgaatatct cctgcatcat tttatttaac gcatcgatcc
    35521 ctgcaggtcc ccgatacatt ggtgctaata cttggatatc ttgtgcacgg aatcccttat
    35581 ttttcgcttt ttccacaatt ttagagatca agggttcaat ttgataagca tcacttcgga
    35641 aataggaacg atctttttga ttctttgtga aatcttttgg cagacgtcct tctttgatct
    35701 catgggcaag aggaataata ctagaaccat caccttgccg ataaatctga ttgagttcca
    35761 ttttgggaat ttgttcgatt tccaaaagat catggagtac ttgaccagga ccgacagagg
    35821 gaagctgatc tttgtctcct actaagataa cttgcatatt cgtcggaatc gctttgaata
    35881 atgtgttagc cagccatgta tcgaccatag acatctcatc aacaatcaaa agtccgcctt
    35941 ctaattcttt cgtactgact gaaggtgttt tttcccgtcc attcagcccc agaagacgat
    36001 ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg tttagctgct cggccagttg
    36061 gtgctgcaag tagaatagga aaaatttctt gggtatattt tgaaggatcc aattccagtc
    36121 cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac agtagttttc cccgtacctg
    36181 gtcctcctgt taaaataaat agaggggaac ggattgcttc agcgatagct tgttcttgcg
    36241 aaggtccata ctgtatgccg aatatttttt cgatacgccg aatattcttc tgcacttctt
    36301 tttcaggata atggatttct tttttacgac tgagaaggcg ctggatcgaa ttaccgatcc
    36361 cccattcaga aaagtacaaa ctgttttcgt aaagattagt ctcttcttgt tgtatctttc
    36421 cttcttcaac aagttggata attacagtag caacctgatc aggctggatt tcaattgggc
    36481 gacttgtttc taaaatacgg atcgtttgct gaagcaatgt ctccgccggt acgtatgtat
    36541 ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat agctgctcga attcttctcg
    36601 gtgaatctgc tgcaataccg atttgttcgg ctagattatc tgcacgtttg aacccgattc
    36661 cttctatgtc ttcgactagc tggtacggat tttcttcgat gatatccaaa gactcgttct
    36721 tataggcctg gtagatactg aaagctaact gactaccgaa cccgtaacga ttcagtccca
    36781 tgatcacttg atccatcccg tggtttagcc gaatcgtctc agcaagcatt tctctttttg
    36841 gcttcgtcaa accggagatt tgttttaata ccgaaggatc agcaatgatt ttttcgatag
    36901 catcttctcc cagcaattct acaatttttt ctgctgtttt cttcccgatt cctgggaatt
    36961 tttcgctgga aagatacgct acgatcccat ttgcagttgt tggcttttct tgctcatatc
    37021 tttcgagttt aagctgcctt ccgtatttag gatgatccac taattctcca taaaaacgat
    37081 aagtctcatt ttcttgaata tcgccgaaac ttccggtgac tacaatctct ttttcacggt
    37141 aatcagctgt ggtttcactg atgtgtacca aaagcacttt gtaaaaatta ctaggatttt
    37201 gaaaaaaaat cgcggcaact gtaccaacga aataaggcat ttcttctggc tgcatcggaa
    37261 gccgcctcct tcttctatag taaaggttct ggtgctgttt cagttcctaa aaaatctgtt
    37321 tggttccata ctttcagttc atatggcaat aatctgcaag gctcttcagt ctctaaaatc
    37381 gtcaaactac tattgaacaa tccacccatt tcacgcaact cacttagctc ttttcctgtc
    37441 atccattgaa tggctgcagt taaagaagct ccatgtccta cgaataaaag tgggccatct
    37501 ttatgttggg caaccgcatc tgctacaaca gtttcgatac gtgtgattgc cgattcaatc
    37561 gtttctccgt gaaaaggaga aggatcatac agatctaaat gattacgcaa attcggcaga
    37621 ttatccggat atttctggta catttcatcg atcgactgac cttctaaagt acccagccct
    37681 agttctctta gcttatctgt ataaaaaatc tctaccggac gagcaagctc tttgttgata
    37741 ccttctgctg tatcacgtgc tcgctttgct gtactggaat aaattgcttt aaaaggaaca
    37801 tattttacct ttttcccaaa tgctttgatt tcttcgtaac tttgtggcaa aagcggagaa
    37861 tcgccattgc tcccttgaaa acgtctttcc tgattccatt gtgtcttccc atgtcgggta
    37921 aaatatagtt ccatacgcag caaactcctg tctttctttt attttactct ataataaact
    37981 gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt ctaaattagg gaatccttgc
    38041 tggcgaagtg cttcatacac tatcattgca gctgtattcg ataaattcaa tgagcggaca
    38101 tgctcatcat tcatagggat gcgtaaacac ttttcttcgt tttcacgcat aaatgtttct
    38161 ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct cttctccatc agcaaaattt
    38221 tcgtcactat atactttatg ggcaaatttt gagatcaaat gtagtttacg attacctaaa
    38281 aatgttaaaa aagcttctaa attactgtgg taagtaatat ttacatcatt ccaataatcc
    38341 aaccctgccc gttttagatg tttgtcatca gttgaaaaac ctagtggctc gattaaatga
    38401 agcggagtat tcgtggctgc acacgtacgg gcaatatttc cagtgtttgc agggattaat
    38461 ggttcaaata aaacgatatg attcatcagg atcattcctc tcatatttat gttcgtttct
    38521 attttctgac aatttgattt ttgcataaaa aaaaacgtat caactcggtg tcagtcactg
    38581 cgagtcgtta cgttagtgaa gctcttctca ctaactttta tcagaacagg aacctgataa
    38641 aaaccaatga aaaaacaact tgttgctaat ataacaccct caactttttt ttgcaacctc
    38701 tactcattaa aaactgctag aaaaacgttt tctcttagtt ccttttaata attctatgtc
    38761 aattgtaatc ttcttcaaag gattcttata taagtctgct ttgattcgct cttctacctg
    38821 ccattctaaa ggtgacatgg ccaaaagatc tttttgattt tcctctggta tctcaaattc
    38881 ataggttaca cgctgacgtt cagttaattt cattgcttca gcaaacttgg ctactacacg
    38941 ttcattggaa taactctgtt tttcaggttg attcggataa aaagcctgac gtagttcctt
    39001 taaataattt tcttgtggca ccaccttcaa cacttctcca tcttctgaga gtacacgttg
    39061 gaattcttgg taatgagaag gcgaaaagat atttaaaacc gcgttgaatg tttgatcagc
    39121 aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct tcggcagatt gttctgtagc
    39181 tgcgtagact ccttcttttg aaatatcaaa tccaacagca ttttgaatct ttttcaattt
    39241 tgaaagttca ttcaagaagc taccttctcc gcagccaaca tctaatagcg agacttcttc
    39301 aggtaaatgc tgcgcaatag tctcaataac gggctgatac atgccagatt ggatcattct
    39361 acgccttgga acgaacattc cttgatcgta ttctgtttta accgcatggg ataaaaaata
    39421 aagtgttcct tttttggaaa ggtcaaaacg gtgattattt ttgcatatca agccattatc
    39481 agactttttt acttcttctt ggcaaaccgg acagcggaac agctgatgat tttctgccaa
    39541 aaactggcgt cctcggtcaa ttttttttaa catagttccc cctcatttct gttttatctt
    39601 accgttagat gctcactttg gcaactgcta aaaatatgat acaatgccta gaagaaagtg
    39661 aaagataaag gagaagatta gatgatcaaa gaattttgcg ctgaaaacta cacatctatt
    39721 cctctagcaa ttgctagagg agccgatcgg atcgaattgt gtgacaactt agcggtcggt
    39781 ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa gttatgctgg ggaaaaagat
    39841 atccccgtaa tgacgattat tcgtcctaga gggggcgact tcgtctataa cgatatcgaa
    39901 ttgaaaatca tgcatactga tttgattgaa gcaaagaaat tagggacgga tggcgtagta
    39961 tttggttgtc ttactccttc aggctggcta gacgaagagg ccttagaact attgatcgaa
    40021 acagcagaag ggttgcagat caccttccat atggcttttg acagtattcc tgaagatcgg
    40081 caattcgaag caatcgattg gctggtagaa catggtgttg accgtatctt gactcatggc
    40141 ggtcctgctg acaaaactat tgtagaaaat ttcccacggc ttaaagaatt gattgcctat
    40201 acagatggcc gcttaatcat tttgcctggt ggtggtgtca ctgcagagaa tgcagcacat
    40261 gtagctaaag aattgaacgt aagcgaagta cacggaacca aaatcgtgga tcttcagtta
    40321 taatcttata aacaaaaaaa catttgcaac aggcataatg cttattgcaa atgtttttgt
    40381 ttttttacat aaaatagccc ataaataaag aagcaatatt gaaataaatc agtacactcg
    40441 tcaaatcgct caaagtcgtg ataaagggac cgcttgctac tgctggatca aatcctagac
    40501 gatccattag catcgggatt aaactacctg caagattcgc cactgtgatt gcaaatagca
    40561 tagccatccc tatgacaaac cccaagataa aattatgttt ccaaaaacct acgattagaa
    40621 aaatcgtgat ccccgtaaca gctcctgtga caacgccggt caagacttca gccaaaacaa
    40681 gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag tcgacgaaca gccacagcta
    40741 gcgactgggt tccagcatta ccggcagttc ctgtaatcag cgaaatgaag actgctaaaa
    40801 tactcgcttc acttaccaag tcttcatagt gactgattaa agaagcggtc gacataccta
    40861 aaaacaacag agtgatcagc caaggcaaac gtcgagacgc tgcctttact ggattttcat
    40921 taatttcttc cacattgacc ccggcaagtc ctgaataatc gctagcagct tcatcatcaa
    40981 taacgtcgat gatatcatcg actgtgacga ttcctaacaa atgatcatca taatcggtaa
    41041 caggaagagc taagaagtca taatctctaa atgtttgtgc cacatcttct tggtcgtcgc
    41101 ctacatgaac tgaaagcact cgttcgctca tcagatcaga aatcattgcg tcgtcatcat
    41161 tcacgattag gtcgcgcaga gaaataaccc ctactagctg accttcttga ttaacgacat
    41221 agatgtaata gattgtttca gctacatctg cttcgttctt cagcacgtac atcgcagagc
    41281 gaacagtttg attggctacg atcgaaacaa attcagttgt catgatcgcg ccagccgttt
    41341 catcctcgta atggagaagt tccttgattt cactggcatc gtctgccgac atcaaactta
    41401 aatatttagc aatttgcttt ttatccagcg tattaagcaa gtcaacagca ttatctgtat
    41461 acatctcaga aagcatatca gctgcatagc taggacgcat ttcatctagg tattctttca
    41521 tatgttcgtt gtcttcttca atcacatcga acatatccgc taattcttta ggcgacagat
    41581 aggaatagat cagctgcctg ttctcttctg taagagattg gtagaattgc ccctgctcat
    41641 agatatgcat ctctaaaaaa ctgtttctga atgcctgaat attattttcc cttaaatctt
    41701 gagtcaactg ggcaaatctt tcctctaatt cttgattttc atccaaacca atctcacctc
    41761 gccttattct tcaaaccgtc tcacaactgc ctccatgtct gcggctaaag gctgcttgaa
    41821 ctccagcaac tcttctgtaa atggatggta aaaacgcaga tcataacaat gaagggcttg
    41881 tctttttatt cccagatcca ttcgcccgcc atacatatca tcaccgagca gggcacagcc
    41941 aattgcggaa aaatgaactc ttatttgatg tgtccttcct gtatgcagct ggatatcaac
    42001 taacgcgatt tctgttagtc gcttctctat ccagtactca gtctgtgccg aacgtccttc
    42061 atcagagacc attcgcttca atagcgaaga ataatctcgg gcgatcggca aatcgatatg
    42121 tccatgtgcc gataaatctg aaagccttcc actgactaag gcctgatatt ttttcacaaa
    42181 cttcttttca cgaagttgca tatctaattt tgcgtgcgca aatccatgct ttgcaaaaag
    42241 catcaaacca gatgtatccc gatctagtcg cgtcaccaca tggattactt ggttttcgta
    42301 accttgtttt ttataataag cctttacacg attagccatt gtcccatttg gatggtattg
    42361 ggcaggaata gacgaaacgc ctgctggttt atttactacc agaacgtgtt catcttcata
    42421 cacgatatcg atcggtgttt catccaaaag tacggtttca tgttctcctt cagcaggaat
    42481 cacaatggtt accttatcat ccttagctag agaaaacaag acattttcta cttgatcatt
    42541 aactttgatt tgaccgcctt gaaattttat tttcgctaac agaccctttg agatcccttt
    42601 ttcttttaaa aaatatttta cttgctgaga atgatctttc tcataatccc atgtaaactg
    42661 catatcaatc ctcactaata aaggcatctt tcacacgatg ccaaaaatgc atatgtcgat
    42721 aagaagcaaa atggatccgt tcttctgcta ttcgataata aattgagcgg atatttgctt
    42781 ttgcgacatt caattgatca accgttacaa gataatcttc tgtattttcc aattttattt
    42841 ctagccattc atcatgggca atcacgatcg gagaacccaa agtacgaaac actcgattat
    42901 tcagtgaagc aatctctgcc aactgaaagg cattgatact aggatggatc acagcaccgc
    42961 cgacactttt attatatgct gtcgaaccag taggcgtaga aatcgtcaat ccatctcctc
    43021 taaaactttc aaacagctcg tttttgatgt atacatcggc taccatcgta cgattggccc
    43081 gtttaatcgt tgattcattc agtgctaaaa aatgctggtc cggtgtttca tccaaataac
    43141 tgatccgaac atccagcaag ggataactaa cactttgttc ccggttcgtt cgtaagctgt
    43201 ctactagttc ttccagctcg taatcccgcc aatccgtgta aaaccctaaa tgacctgtat
    43261 gcacgcccaa gaaacgcaca tcattcagac aatgactgta gtgatggaac gccgacaata
    43321 atgtgccgtc tccgccgact gagatgacca attctggctc atgatcatct cgttgaatcc
    43381 ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc tatagtcttt gcttctttat
    43441 tatgaacgat cgctactttc atcccctgcc tcctctcttt cgctttcttt cttcaaaagc
    43501 gttcgatagt agacatcatc tgattttcct cttccatggg agaataaccg ctgtgcttct
    43561 tggatttctt cacggatact ggacatctct tcatccaact gataggcagc ttctgccgca
    43621 cgctgaagtc gttcactcat ttcttctgga aaaacgcctt gatatttata attcagcgaa
    43681 tgctcaatcg ttgcccagaa attcatcgca agagtccgaa tctggatttc cgccaaaatt
    43741 ttcttctcac cgctgatcag ctgtacagga tactcgatca ccagatgata agaacggtag
    43801 ccacttgctt ttttatttgt gatgtaatct cgttcttgga gaactttcat gtcttttctt
    43861 ttacggatga tctcaacaac ttgatggata tcttctacaa actgacacat gatccttaac
    43921 ccggcaatat cagacatttc ttcttctaaa cgtcccattg gaatatgccg taattgtgat
    43981 tttgttacaa tactatcgac tggcttgact ctcccagtga caaattcaat cgggacatgt
    44041 cgattttgtt cacggaactg tttgcggatc ccccgcagtt tcactttcaa ttctgataca
    44101 gtttgttcat atggtgctaa aaatgcttcc caatctcgtt ccatagctga tacctctact
    44161 tcattaacat tcattgttta ttctaccata tatgcaaagt tttttcgtac tcttgattta
    44221 tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa aaggagtcga tcaaatgaca
    44281 caaatgcttg aaatcgaata taaatctatg ctgacaaagc aagagtacga gatattcatc
    44341 gctcactatc aattaacaga taaagatttc cgtgtacaga ctaatattta tttcgatacc
    44401 gttgatgaac agctgaagaa acaaaattgt ggcctacgga tccgtttcgt tggtcatcaa
    44461 gctgaatata cattaaaaac acctgctgaa aaagggcgtc tcgaaacaac ggataccttt
    44521 ataacagaag aagcagaagc atttatcttc gaaaaacgtc tgcctcgttc aggcgcagtt
    44581 tttcaaaaac tttctgattt gaatatcgat cctgcttcat taatccaaac cggaaagctt
    44641 acaacaaaac gggctgaatt ccctattgaa gaaggacttt tagctattga tgaaagctgg
    44701 ggagacaatc tacacgattt tgaactggaa ctagaggtag gggatgcatc tgtgggaaaa
    44761 attgctttta taaatttttt aaaacgtttt tctttacctt atcgtcctgc taagaacaag
    44821 atccaacgta tgcttgaagc taaaaattag aaacctttca ttttcacaag gcacttgagt
    44881 tcattttttt acttttttct gttaagttat aggatgtgaa agaatctagt cactagttgt
    44941 atagaatcta ttttggggga gattcatatg atcgaaatct atttatttgt taatccttta
    45001 ggggaatact gtttcgaaat ggaacaacag ttattacaat ttatcgaaga agagtacggg
    45061 acaccatcaa aagaaaagat gcaatttcgc tttcttcctt tggtcaattt gcagacaatt
    45121 ggggatgtca tgcaacaaaa aggaatttct cagaacaatc ttgaggaacg taatcactta
    45181 ttctcaatga cttattctgc agcattggac tgcaaagcgg cgcagtttca aggaaagaaa
    45241 aaaggccgcc gttttttgat caaattgcaa gaagcagtcg gctgtaatga cataccatat
    45301 tctcaagaac ttgctgaatc catatttgaa gaaatcggcg gcgatttgaa tatgtttaaa
    45361 gaagatcgtc agtctgactt cgttaaagaa gtattctttt ctgatcaaaa aattgctcgg
    45421 gaaatgggca tcacaaaaca cccttctgca gtcgtttaca attatgcctg cgaccgtgat
    45481 tttggtgttt tagtagaagt aaaaggtaca agtagccttt ggagaatcac agagctgtgt
    45541 aaaacaacag ataactcata tcaagttttt aatggtgaac atttacatac aaatgattct
    45601 cagttccatt caagaactag ccatttacac ttgctttcta attaaaaaaa tacgtcgact
    45661 cccttacaag agagccgacg tatttttttc gtttctattc gagaaaatca aaaatagaaa
    45721 gccttataaa cgatcgatca attcttctaa ttcatttaga cgttgttcaa acatcgacat
    45781 tgcatcttca atatatgcag cctgtgtcat atcaactcct gcttttttca ttacttccac
    45841 aggataatcg ctgttacctg cttttaggta agccaagtag ttctctagtg cctctggttc
    45901 ctggtttagt attttcttcg ccagtgctga agcagcagaa aagccagtcg agtattggaa
    45961 aacatagtaa ttataataaa aatgcggtat ccgtgaccat tcgaatttaa tttctgggtc
    46021 ttcttcgact gctggaccat aatattttgc attcaattta ccataactat cacttaggta
    46081 ttcactagtc aatggcacac ctttttcatc ttccgtatgc atgaaatgct cgaattcagc
    46141 gaattgtgtc tggcggaaaa ctgttccttt aaacccatcc aagtaatggt tgagtacata
    46201 agcccgtaca cgagggtctt tttctgtttc caataaatac tccgttagga tattttcatt
    46261 cgttgtcgaa gcaatttctg ctaaaaagat ggagtagtcg ccgtacacat aaggctggtt
    46321 cgaacgagtg aaataactat gaacactatg tcccatttcg tggacaagcg taaatagctg
    46381 atccagcgta tcatgccaat tcaataaaat atatggattt gtgtcatagc ttcccgaaga
    46441 ataagcaccg cttcgtttcc ctttattttc gacaacatcg atccaacgtt cagagaatgc
    46501 tttttctacg atggccatgt attcttcacc cattggtttc agtgcttcta aagctttttc
    46561 ttttgcttct tcgtacgtaa aggtaattgg agcttcccct aagaccggtg tataaagatc
    46621 atacatgtgc agtttttcca cttctaataa acgtttccgt aattccatgt atcgatgtaa
    46681 caaaggcaaa tgcttgttta ccacgtctac caaagtatcg tatacacttt caggaatatg
    46741 attattgctc aaagacgctt ctctggcaga gctgtaatta cggactttcg ctttaaaatt
    46801 atgtcctttt atatgtgtgc ctaaagtaga agcaaatgta tttctaaatt gttcgtaaac
    46861 actgtacaat cccttaaatg ctgcttcgcg cacccttcga tccgtgcttt ctagcaactg
    46921 accatacacg ccatgagata attggactat ttcaccattt tctccttcaa tcgttggaaa
    46981 aactagatct gcattattca aaacagcaaa tgtatcactt gaagcatcaa agatttcacc
    47041 tgctccagca agaagagatt cctgctcagc agataagaca tgggctcgat tatctactat
    47101 ttgctggata taatggcgat agacttccaa ttttggttct tctttgaaat attgccaaat
    47161 ctggtcatct gacaattgca atatttctgg ttcaaaccag gaaacggctt cactaacttt
    47221 cgaaaacaga ctgcttgctc ttgcataaag cgcttggtaa tctgtatttc cagtgtcttg
    47281 atcgttttta aggtgcgcat atacataaat gacttcagtt tggcgataaa ccctcaatac
    47341 gaattcaatc gcatttaaaa attgagaagc gccttgatca agtgttcctt tgtgtttttc
    47401 agattgtttt aactcttctg ataattccaa atatttctca tcaaactctt ggtcgcttga
    47461 aaagatcttg gtcaagtccc aagttaaatt ttcaggcaat tcttctcgtt ttggcaactg
    47521 ctttacttcc atgaaatcca ctccttttct tcccttagct ctatctatca aaatagatca
    47581 tagtctaaca tccacatgtc acagcatcta acttttctac tcattcatgt gtctgcttcc
    47641 atcaatttta gcataactaa atcttgtttt acatagaaaa ccgattccta gcctattttt
    47701 atacttacta ttttttcaaa gaagacaagt gtatgcattc gttaaaaaac tgctgataga
    47761 ccatctcttc atcaatcata ggaaaaagcc attccctttt atgttctttt atttggcaaa
    47821 gccatgagtt gaaatcagga actttctggt tttccaaggc ttcttcgtac aacattcgat
    47881 aaagaaaaac ctgttcttgt aaataaaaga aaaaccgact tttttgatag atccacgatg
    47941 atagatgcaa aagatgttta tgacgcaagt agcattgttc ttgtatggat actgtattct
    48001 tttgcttgtg aataagttgg cgtgtcagcc atttcttccc atcttcactc actttttcta
    48061 tttttttcat ttggttcatt ttaatttcgt tactagtgaa tatttcttta aggtccctac
    48121 tagaaaaagt ccagcagcta ctgcaaaagg aaatatgccc tgatacagtc tctgtaatat
    48181 gatgatacaa ataaagcttt tgtttcttga gatcagcttt ccagcaatgc actcctcttt
    48241 ttctattata gtagcagcaa cttttttcta tcaatgagaa ttgcgattgt ccaagaaaag
    48301 aattaccaag tatccaccat acttggtaac tgttattcaa ataattttgg gttctttcaa
    48361 taaatctctg gtgagacagt ctagaacatt ggatttctat tgctagattc gccaacaaaa
    48421 tatccggcct ttgccgcaac ctcggcaaat aggcttccaa aaatactggt tctactgact
    48481 gtcccagcca atccatgaag cattccttta actgtaagtg ctcttttgat tctctttcac
    48541 taaagaccga acagttatct gatttttgat gggcaaaatg agcacatttt ttctttccct
    48601 ttttcaaaat cactcttttt cgacagccac gacaataata tttctttcct ttttctgcat
    48661 gtttagcaaa acagcactta ttatattcat tcattgcaag cagcattttt ctccctctga
    48721 acttatttac gtctaagaat ttatttacac gaaaaaagag ggcgtgaaaa agctattctg
    48781 catcaagtaa taagaacagc taaacaaaaa tagttcccaa tatttttcgt ttggttgggc
    48841 ttgttaccgc agatgcaagc ttttgaacac atttatacga aaaaaagact gcgacacaat
    48901 ttttgtcaca gtctcttact ataatgctga tttatacaaa ataaaatttc gttcgctcga
    48961 tagcattacg cgccatcaga cattgtccat attcattcaa tgtttctggt gtacgatcgc
    49021 tcttttcagc aaactccaaa attcgagcaa tctgattctc tacatccgct tggttgagat
    49081 tttccatcca gaagtgaacg actaggtaat agcgctcttc gaataaatac aaatcagtcc
    49141 aagcagactc tagttcgaca tctgttgcaa gttggatcac ttgttcaaaa tcttcaaaag
    49201 caaaagttgc ttgtctttcc tgttcttcta gggcgcttcc agataattct tcctccggat
    49261 cgctgccaat aatctgctga cgaagaaagt cactcacatc ttctgaatca acagtagaat
    49321 cttctgattg agaaatttct tcatccggaa gatttttact gataaacagt tctaatccgt
    49381 ctccttttgg caaaacttgg aaagtgacag cttcgcttcc tcggaactcc tcttcgatat
    49441 caacttcttc caaaatacta taaaagaaat tttcgatttc tttgtgattg ccaagtaaat
    49501 ccaaaaatga aacgccccgt tcctctaaat cttcatgacc aattagtaca cggatcgtat
    49561 tttcattaat atgttccatt tccattttgc tacacctcac tttgccacaa tgtttaatat
    49621 agattcattg tagcgaaatc agcttaaatg taaagaaaaa aaatctcgct gttaagcgaa
    49681 tttaataaaa ccagcggatt tttcaacctt tcaagctatc ttatttaact ataacatttt
    49741 tggcagacga atactatata gccgactttt cagctaattt tccctctcta tttttcgtgc
    49801 aatcatcatc ttctactacc tttttataca tctaatcaag aatacttttt gtaaaaacaa
    49861 aaaaacttgg aaaaactatc tttgtaatag t
//
LOCUS       DACEKG010000012        45192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEKG010000012 DACEKG010000000
VERSION     DACEKG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.012"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..2095)
                     /locus_tag="IW664_000614"
     CDS             complement(23..2095)
                     /locus_tag="IW664_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0848001.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IW664_000615"
     CDS             complement(2028..2405)
                     /locus_tag="IW664_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0848002.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IW664_000616"
     CDS             complement(2534..3094)
                     /locus_tag="IW664_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0848003.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IW664_000617"
     CDS             3244..4536
                     /locus_tag="IW664_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0848004.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IW664_000618"
     CDS             complement(4596..5714)
                     /locus_tag="IW664_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0848005.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW664_000619"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW664_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0848006.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW664_000620"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW664_000620"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0848007.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IW664_000621"
     CDS             8920..9519
                     /locus_tag="IW664_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0848008.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW664_000622"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW664_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0848009.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IW664_000623"
     CDS             complement(11214..11558)
                     /locus_tag="IW664_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0848010.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IW664_000624"
     CDS             complement(11695..12831)
                     /locus_tag="IW664_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0848011.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IW664_000625"
     CDS             complement(13020..13994)
                     /locus_tag="IW664_000625"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0848012.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IW664_000626"
     CDS             complement(14074..14949)
                     /locus_tag="IW664_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0848013.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IW664_000627"
     CDS             15079..16986
                     /locus_tag="IW664_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0848014.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IW664_000628"
     CDS             complement(17527..20805)
                     /locus_tag="IW664_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0848015.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IW664_000629"
     CDS             complement(21076..21621)
                     /locus_tag="IW664_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0848016.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IW664_000630"
     CDS             complement(21633..22325)
                     /locus_tag="IW664_000630"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0848017.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IW664_000631"
     CDS             complement(22344..22628)
                     /locus_tag="IW664_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848018.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IW664_000632"
     CDS             complement(22641..23204)
                     /locus_tag="IW664_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0848019.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IW664_000633"
     CDS             23432..24094
                     /locus_tag="IW664_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0848020.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IW664_000634"
     CDS             24218..25414
                     /locus_tag="IW664_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0848021.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IW664_000635"
     CDS             complement(25515..26891)
                     /locus_tag="IW664_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0848022.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IW664_000636"
     CDS             complement(26928..27794)
                     /locus_tag="IW664_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0848023.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IW664_000637"
     CDS             complement(27883..28908)
                     /locus_tag="IW664_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0848024.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IW664_000638"
     CDS             complement(28926..29600)
                     /locus_tag="IW664_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0848025.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IW664_000639"
     CDS             complement(29784..30677)
                     /locus_tag="IW664_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0848026.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IW664_000640"
     CDS             complement(30751..32448)
                     /locus_tag="IW664_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0848027.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IW664_000641"
     CDS             complement(32441..33754)
                     /locus_tag="IW664_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0848028.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37272)
                     /locus_tag="IW664_000642"
     CDS             complement(33754..37272)
                     /locus_tag="IW664_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0848029.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37565..38023)
                     /locus_tag="IW664_000643"
     CDS             complement(37565..38023)
                     /locus_tag="IW664_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0848030.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38321..38839)
                     /locus_tag="IW664_000644"
     CDS             complement(38321..38839)
                     /locus_tag="IW664_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0848031.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(38841..39458)
                     /locus_tag="IW664_000645"
     CDS             complement(38841..39458)
                     /locus_tag="IW664_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0848032.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39455..40309)
                     /locus_tag="IW664_000646"
     CDS             complement(39455..40309)
                     /locus_tag="IW664_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0848033.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40306..41217)
                     /locus_tag="IW664_000647"
     CDS             complement(40306..41217)
                     /locus_tag="IW664_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0848034.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41258..42592)
                     /locus_tag="IW664_000648"
     CDS             complement(41258..42592)
                     /locus_tag="IW664_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848035.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            42800..43630
                     /locus_tag="IW664_000649"
     CDS             42800..43630
                     /locus_tag="IW664_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0848036.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(43874..44123)
                     /locus_tag="IW664_000650"
                     /pseudo
     CDS             complement(43874..44123)
                     /locus_tag="IW664_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44314..44508)
                     /locus_tag="IW664_000651"
     CDS             complement(44314..44508)
                     /locus_tag="IW664_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848037.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        13071 a         9722 c         7970 g        14429 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgtggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg ttaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta tcattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttccttttct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    37561 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    37621 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    37681 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    37741 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    37801 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    37861 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    37921 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    37981 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    38041 aaaaatattt gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta
    38101 atttaatttt aattttaaat caaatgatat cctttttagt taaaaaacac caattataca
    38161 acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa caaaacttaa
    38221 gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa
    38281 atgcgcattt acgcactcat gctttattcg tacaattaat tcagataacg gcagcgcata
    38341 ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa
    38401 gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa
    38461 ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag
    38521 gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag
    38581 gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac
    38641 tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag
    38701 caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga
    38761 tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt
    38821 tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg
    38881 ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt
    38941 ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg
    39001 cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc
    39061 agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta
    39121 gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg atagtcgatc
    39181 aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaag agagaagttc
    39241 tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga
    39301 taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac
    39361 aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa
    39421 taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat
    39481 ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga
    39541 caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt
    39601 gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga
    39661 aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca
    39721 atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt
    39781 cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat
    39841 tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga
    39901 ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca
    39961 aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag
    40021 aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat
    40081 agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg
    40141 ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca
    40201 tccaaagcaa tagaatgatc aaaatgattc ctattactgt catgaggaca taacttagta
    40261 ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc
    40321 tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca gaaccaacac
    40381 gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc
    40441 cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt
    40501 tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac
    40561 atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac
    40621 gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc
    40681 taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt
    40741 cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt
    40801 gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc
    40861 tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg
    40921 attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac
    40981 gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt
    41041 gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc
    41101 aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga
    41161 gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt
    41221 ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt
    41281 tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa
    41341 tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg
    41401 tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg
    41461 ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac
    41521 ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag
    41581 aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc
    41641 aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg
    41701 gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc
    41761 cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta ttgatcgtat
    41821 ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag
    41881 tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg
    41941 cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc
    42001 cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat
    42061 cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat
    42121 ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg
    42181 gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg
    42241 cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag
    42301 ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta
    42361 catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc
    42421 gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt
    42481 cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta
    42541 ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc
    42601 tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa
    42661 taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat
    42721 tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa
    42781 tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc
    42841 agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg
    42901 cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa
    42961 atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc
    43021 gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca
    43081 aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc
    43141 tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat
    43201 tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag
    43261 acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc
    43321 tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt
    43381 agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa
    43441 agacattgct tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg
    43501 ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat
    43561 cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt
    43621 caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca
    43681 aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta
    43741 taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc
    43801 atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct
    43861 taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt
    43921 gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc
    43981 cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg
    44041 atctcctgaa tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg
    44101 aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct
    44161 ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa
    44221 gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa
    44281 tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt tataaatgga
    44341 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
    44401 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
    44461 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
    44521 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
    44581 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
    44641 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
    44701 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
    44761 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
    44821 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
    44881 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
    44941 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
    45001 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
    45061 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
    45121 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
    45181 tttagaattt tt
//
LOCUS       DACEKG010000013        44711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEKG010000013 DACEKG010000000
VERSION     DACEKG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.013"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            102..1370
                     /locus_tag="IW664_000652"
     CDS             102..1370
                     /locus_tag="IW664_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAQ0848038.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1378..2784
                     /gene="lpdA"
                     /locus_tag="IW664_000653"
     CDS             1378..2784
                     /gene="lpdA"
                     /locus_tag="IW664_000653"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0848039.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2946..3221
                     /locus_tag="IW664_000654"
     CDS             2946..3221
                     /locus_tag="IW664_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0848040.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3227..3994
                     /locus_tag="IW664_000655"
     CDS             3227..3994
                     /locus_tag="IW664_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0848041.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4162..5991
                     /gene="typA"
                     /locus_tag="IW664_000656"
     CDS             4162..5991
                     /gene="typA"
                     /locus_tag="IW664_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0848042.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6252..8024
                     /gene="recQ"
                     /locus_tag="IW664_000657"
     CDS             6252..8024
                     /gene="recQ"
                     /locus_tag="IW664_000657"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0848043.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8138..8440
                     /locus_tag="IW664_000658"
     CDS             8138..8440
                     /locus_tag="IW664_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0848044.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8443..9606
                     /locus_tag="IW664_000659"
     CDS             8443..9606
                     /locus_tag="IW664_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0848045.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9632..13060
                     /locus_tag="IW664_000660"
     CDS             9632..13060
                     /locus_tag="IW664_000660"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0848046.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13081..14205
                     /locus_tag="IW664_000661"
     CDS             13081..14205
                     /locus_tag="IW664_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848047.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14195..14632
                     /locus_tag="IW664_000662"
     CDS             14195..14632
                     /locus_tag="IW664_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0848048.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14616..14954
                     /locus_tag="IW664_000663"
     CDS             14616..14954
                     /locus_tag="IW664_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0848049.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15073..15633
                     /gene="rsmD"
                     /locus_tag="IW664_000664"
     CDS             15073..15633
                     /gene="rsmD"
                     /locus_tag="IW664_000664"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0848050.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15630..16121
                     /gene="coaD"
                     /locus_tag="IW664_000665"
     CDS             15630..16121
                     /gene="coaD"
                     /locus_tag="IW664_000665"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0848051.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16111..17154
                     /locus_tag="IW664_000666"
     CDS             16111..17154
                     /locus_tag="IW664_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0848052.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17313..18041
                     /locus_tag="IW664_000667"
     CDS             17313..18041
                     /locus_tag="IW664_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0848053.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18157..18402
                     /locus_tag="IW664_000668"
     CDS             18157..18402
                     /locus_tag="IW664_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0848054.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18399..18617
                     /locus_tag="IW664_000669"
     CDS             18399..18617
                     /locus_tag="IW664_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0848055.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18753..19292
                     /locus_tag="IW664_000670"
     CDS             18753..19292
                     /locus_tag="IW664_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0848056.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19285..21558
                     /locus_tag="IW664_000671"
     CDS             19285..21558
                     /locus_tag="IW664_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0848057.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21596..22642
                     /gene="holA"
                     /locus_tag="IW664_000672"
     CDS             21596..22642
                     /gene="holA"
                     /locus_tag="IW664_000672"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0848058.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22731..22985)
                     /locus_tag="IW664_000673"
     CDS             complement(22731..22985)
                     /locus_tag="IW664_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0848059.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23385..23654)
                     /locus_tag="IW664_000674"
     CDS             complement(23385..23654)
                     /locus_tag="IW664_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848060.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24124..24579)
                     /locus_tag="IW664_000675"
     CDS             complement(24124..24579)
                     /locus_tag="IW664_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0848061.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24717..26645)
                     /locus_tag="IW664_000676"
     CDS             complement(24717..26645)
                     /locus_tag="IW664_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0848062.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26842..27675)
                     /locus_tag="IW664_000677"
     CDS             complement(26842..27675)
                     /locus_tag="IW664_000677"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0848063.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27867..28571)
                     /locus_tag="IW664_000678"
     CDS             complement(27867..28571)
                     /locus_tag="IW664_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0848064.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28594..29220)
                     /locus_tag="IW664_000679"
     CDS             complement(28594..29220)
                     /locus_tag="IW664_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0848065.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29546..31921
                     /locus_tag="IW664_000680"
     CDS             29546..31921
                     /locus_tag="IW664_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0848066.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31945..32436)
                     /locus_tag="IW664_000681"
     CDS             complement(31945..32436)
                     /locus_tag="IW664_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0848067.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32623..33543
                     /locus_tag="IW664_000682"
     CDS             32623..33543
                     /locus_tag="IW664_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0848068.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33697..34041
                     /locus_tag="IW664_000683"
     CDS             33697..34041
                     /locus_tag="IW664_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0848069.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34231..34731)
                     /locus_tag="IW664_000684"
     CDS             complement(34231..34731)
                     /locus_tag="IW664_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0848070.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35259..35342
                     /locus_tag="IW664_000685"
     tRNA            35259..35342
                     /locus_tag="IW664_000685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35293..35295,aa:Leu,seq:cag)
     gene            complement(35666..36304)
                     /locus_tag="IW664_000686"
     CDS             complement(35666..36304)
                     /locus_tag="IW664_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0848071.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            36639..37073
                     /locus_tag="IW664_000687"
     CDS             36639..37073
                     /locus_tag="IW664_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0848072.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            37096..37416
                     /locus_tag="IW664_000688"
     CDS             37096..37416
                     /locus_tag="IW664_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848073.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            37557..37748
                     /locus_tag="IW664_000689"
     CDS             37557..37748
                     /locus_tag="IW664_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848074.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(37819..38076)
                     /locus_tag="IW664_000690"
     CDS             complement(37819..38076)
                     /locus_tag="IW664_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0848075.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            38234..38497
                     /locus_tag="IW664_000691"
     CDS             38234..38497
                     /locus_tag="IW664_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848076.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            38521..38709
                     /locus_tag="IW664_000692"
     CDS             38521..38709
                     /locus_tag="IW664_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848077.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(38792..38977)
                     /locus_tag="IW664_000693"
     CDS             complement(38792..38977)
                     /locus_tag="IW664_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848078.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            39192..39563
                     /locus_tag="IW664_000694"
     CDS             39192..39563
                     /locus_tag="IW664_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0848079.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(39713..39785)
                     /locus_tag="IW664_000695"
     tRNA            complement(39713..39785)
                     /locus_tag="IW664_000695"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39750..39752),aa:Gln,seq:ctg)
     gene            complement(39831..40259)
                     /locus_tag="IW664_000696"
     CDS             complement(39831..40259)
                     /locus_tag="IW664_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848080.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(40319..41077)
                     /locus_tag="IW664_000697"
     CDS             complement(40319..41077)
                     /locus_tag="IW664_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0848081.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            41372..41569
                     /locus_tag="IW664_000698"
     CDS             41372..41569
                     /locus_tag="IW664_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848082.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            41675..42157
                     /locus_tag="IW664_000699"
     CDS             41675..42157
                     /locus_tag="IW664_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848083.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(42215..42811)
                     /locus_tag="IW664_000700"
     CDS             complement(42215..42811)
                     /locus_tag="IW664_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0848084.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSDGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            43198..44664
                     /locus_tag="IW664_000701"
     CDS             43198..44664
                     /locus_tag="IW664_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0848085.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        14769 a         7669 c         9153 g        13120 t
ORIGIN      
        1 cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca gaatcagctg
       61 gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa ggaatcgcag
      121 aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa gatgatacat
      181 tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc acagggacag
      241 ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta ttagtagaaa
      301 tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct gaagcaccaa
      361 aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat ccaaataaac
      421 gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta gatatctcac
      481 aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa aacttcttgg
      541 caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag
      601 aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc aagtccaact
      661 taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc gctaaagcaa
      721 tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta gaagtgtcta
      781 aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt acgaaactta
      841 ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac ccagtattga
      901 atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac aatatcggta
      961 ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca gaccgcaaag
     1021 gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat gacggcaaac
     1081 tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga tctgtaggtg
     1141 gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt gtaggaacga
     1201 ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac
     1261 tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata
     1321 atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga taaacaaatg
     1381 gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg acctggtggc
     1441 tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag
     1501 tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact gatagctgca
     1561 ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag tgaaaacgtc
     1621 aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca tacgttgact
     1681 tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc
     1741 ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac ttattcgttc
     1801 aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt taaattcgga
     1861 ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa gaaatttgtg
     1921 atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca
     1981 gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa agatctagta
     2041 aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg
     2101 aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa
     2161 gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat
     2221 atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac ggtggataaa
     2281 caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc aggggcggca
     2341 ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa
     2401 gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga attggcttct
     2461 gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc atataaattc
     2521 ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt catccgtcta
     2581 gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt
     2641 gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga agatatcgcc
     2701 ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc ggaattagca
     2761 ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac tgaaaaatca
     2821 gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa aaatatgaca
     2881 acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa ataggagagt
     2941 tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac gaaatggtca
     3001 ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata aacaatgaag
     3061 cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt gaggaaaaac
     3121 gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca gtgcagcaag
     3181 caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg aacagatgat
     3241 tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt ctcaacaaac
     3301 attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg atcgaaacat
     3361 ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa tattggcaga
     3421 agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca tcgatcctat
     3481 tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc tcgctgttta
     3541 tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg aacttttttg
     3601 gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac cagcaaataa
     3661 atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata ataaatatgg
     3721 aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg ctggtcttga
     3781 actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta gcccttggga
     3841 ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg gtctttttgg
     3901 tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt gtgcatatgc
     3961 agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc attcacaaaa
     4021 atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt tactatattt
     4081 catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca taaagaacca
     4141 ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt cgcaattatt
     4201 gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca atcagataca
     4261 ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct agaaaaagaa
     4321 cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat tcgcgtaaac
     4381 atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat catgaaaatg
     4441 gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca aacacgtttt
     4501 gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa caagattgac
     4561 aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt catcgaatta
     4621 ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact aaatggtacg
     4681 tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat ttttgataca
     4741 attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca attccaagtt
     4801 tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt atttagagga
     4861 acaatcaaag taggagacca agtagcatta attaaattag acggaacagt taagaaattc
     4921 cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca agaagcaaaa
     4981 gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga aacagttact
     5041 cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac attgcaaatg
     5101 actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt aaccgctcgt
     5161 aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg agttgaacca
     5221 acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt gtctatcttg
     5281 attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga agttattgaa
     5341 aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga tacaccagaa
     5401 gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat gcaagatatg
     5461 gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg cggtttgatt
     5521 ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa ccatactttt
     5581 gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg ggcgcttgtt
     5641 tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga acgcggtaca
     5701 gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga aaacagccgt
     5761 gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt acggtctgct
     5821 actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga agaatctttg
     5881 gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg cttgagaaaa
     5941 caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata atctgtaaaa
     6001 ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt tgtccaactc
     6061 ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac gccgaaaggc
     6121 gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa taagataatg
     6181 tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg gcaagagaaa
     6241 ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag tttagacctg
     6301 gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga atcatgccta
     6361 ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat gggcttactg
     6421 tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg aatcaattag
     6481 gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct agacgcttcc
     6541 aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt tttatcatgc
     6601 cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt gacgaggcac
     6661 attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg gcgaaccagc
     6721 tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg actgtgcaag
     6781 tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt cagactggtt
     6841 ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa caatatctaa
     6901 tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca acgagaaaag
     6961 aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt agatatcatg
     7021 gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac gatcgcttgc
     7081 agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat gtccgttttg
     7141 ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct ggtcgggcgg
     7201 gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat atccaagtcc
     7261 aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa gaatatgaaa
     7321 aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa tacatcctaa
     7381 actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta gatgacagag
     7441 aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa cgtatgggtg
     7501 agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa caaaagctgc
     7561 aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa tcgcaaaaag
     7621 aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc atcaatggtg
     7681 agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga caagaatctg
     7741 tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc gatacactat
     7801 ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt cccccgtatg
     7861 tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca agcagattgg
     7921 agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc gaagcctttt
     7981 tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa cgaatacgta
     8041 aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa aatcaaatat
     8101 gttataatta aggaattaga atgggaggta agcacagatg gattctttgt cgaaaaaact
     8161 tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc gaaatcaaaa
     8221 caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata cacagatgta
     8281 cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa agaacgaggg
     8341 ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg aggtttacca
     8401 agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa gaaagtgaaa
     8461 aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg tgtattggga
     8521 ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca aaatccgtcc
     8581 gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat cgcactactt
     8641 tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc catcgcagtt
     8701 ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg ggcgaaaggt
     8761 tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat catttctatc
     8821 tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga ttttcttggg
     8881 actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc tattcttccc
     8941 gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt agccagcgga
     9001 gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag tacttttacg
     9061 atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca gtatatctat
     9121 aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg gcatcaacta
     9181 gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt aggtaacagt
     9241 atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc aatcgtagtg
     9301 gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt tatgattgtg
     9361 cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt gtgcattggg
     9421 atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac tggcgttatt
     9481 cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt gctgatgctt
     9541 tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa gagcttaggt
     9601 ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg ctaacagggg
     9661 tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa ctgttggaat
     9721 ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag catatttagt
     9781 aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca tttctatcgc
     9841 gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg aaaacttgaa
     9901 ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac tgcatcattt
     9961 agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg ggattgcttc
    10021 gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt ttgcgaaaca
    10081 atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg gcatgcgtgt
    10141 agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg aagcaaaagc
    10201 agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta agcatatcga
    10261 agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa gagattgttc
    10321 tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta tgaatgaaca
    10381 gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg ggtatgtcaa
    10441 cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg aagtcaaccc
    10501 tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata tcgtaacaac
    10561 gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc ctcagcaaga
    10621 aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag acccgttgaa
    10681 taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg gttttggcgt
    10741 acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt ttgattcatt
    10801 gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga aaatggaacg
    10861 ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta tgctcaatgt
    10921 catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg atagtacagc
    10981 aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga aatatatcgg
    11041 tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat tttatgaaga
    11101 accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta agaatgtatt
    11161 agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa atttgttgtt
    11221 gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg ttcggacaaa
    11281 ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct tttcaagtga
    11341 gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag atccttggca
    11401 acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc ttttcagagg
    11461 ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat ttgtcaaaga
    11521 agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact ggactccaca
    11581 aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg cagctatttg
    11641 ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt attataaaga
    11701 catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag atatggctgg
    11761 gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa cgacagatct
    11821 gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt attctgctgc
    11881 ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg ggaatactag
    11941 tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc tgccagaaat
    12001 cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca tgtattacaa
    12061 accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg aaatgccagg
    12121 aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc atcgctggga
    12181 tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg ttaaagtaac
    12241 tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg atcttacaga
    12301 agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg tgactttctt
    12361 ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa agattattct
    12421 taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag tcgacttcaa
    12481 cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag acgaagtatt
    12541 aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata atcaatttgg
    12601 cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg gtgaaacaat
    12661 caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa tcggagaacc
    12721 cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac gtgaaatcac
    12781 tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg aaccgacaaa
    12841 ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag tgaaaaaagg
    12901 cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa tggaaacggc
    12961 catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag gagacactat
    13021 ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag gtggggaaac
    13081 atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc attctacatg
    13141 gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca acaaatatca
    13201 aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc tacttatatc
    13261 ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg attaactagc
    13321 ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac atttgaagcg
    13381 aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc tgctgatatc
    13441 gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac tatctatact
    13501 aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga agaggatatg
    13561 aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt ttttgatcaa
    13621 acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt gaaactattg
    13681 ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc ggctgattgg
    13741 gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat gcttagaaga
    13801 gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag tcagctgcta
    13861 ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat ggctgattgg
    13921 caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa tgatgaattg
    13981 gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat tcatccggcg
    14041 attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca tgaccgtttc
    14101 atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat gttagtctta
    14161 ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag agctgtttca
    14221 actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa cgatggcttc
    14281 ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt tgaaagaagc
    14341 gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt atgcacctga
    14401 ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata tggatgagca
    14461 tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc aaattgtcca
    14521 agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc cgttttttga
    14581 aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat agtgaatttg
    14641 aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa ttaaagaatc
    14701 tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc gtgctttact
    14761 taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat tttgttcgaa
    14821 acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa ataggaaaca
    14881 aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa atccgattag
    14941 ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac ttggataacg
    15001 atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt accatttcta
    15061 aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt aaaaagttta
    15121 gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt caatatgatt
    15181 ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg tggtttagct
    15241 atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa ttaccaagcg
    15301 ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt taccacaata
    15361 aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca attcgactac
    15421 ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca taaaatggaa
    15481 gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa aacagtcgaa
    15541 ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg cataacggcc
    15601 gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca ggaagttttg
    15661 atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta ttcgatgagg
    15721 tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca gaagaaagaa
    15781 tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg catcaagaaa
    15841 atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc agaggcatac
    15901 gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat catctatatg
    15961 atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg agttccagta
    16021 ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg ccagtgatca
    16081 acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt gaaaacagta
    16141 ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt gccgtattat
    16201 gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa tgataaggaa
    16261 gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc tacaggtttc
    16321 ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga agaattgact
    16381 ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga ttcttcccaa
    16441 aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat ggaattcaaa
    16501 ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct ttcagttgga
    16561 gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt tatggattat
    16621 gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa tggtgaagaa
    16681 aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg aataggtatc
    16741 acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa ttctggctct
    16801 atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca agtcactggt
    16861 aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag tgaaggagaa
    16921 gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc tggcgtagag
    16981 attttctttg caccagatga tacgatttca gacgatgtta agaaagaata tcctgaaata
    17041 aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc aatgaaaatc
    17101 gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa atgaaactaa
    17161 ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc gtcaaaaatc
    17221 ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg aagggacgat
    17281 tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat gatttttact
    17341 ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata ctagcttcac
    17401 ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca agtggttttt
    17461 tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa gaaatacttt
    17521 cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta gaagaggatg
    17581 ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct ggtatctatt
    17641 ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga ttattagaag
    17701 aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa gtcctatata
    17761 tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc tcggaaggaa
    17821 aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc actccatcag
    17881 aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt cgatttagag
    17941 aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt atcggtgaaa
    18001 agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca aaatcgactg
    18061 gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta agagcttggc
    18121 aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa agaaacatta
    18181 aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca tattatgaat
    18241 acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa agatgtactt
    18301 agctatacag cagataaagt atttattctc attgggacga atgatctagc agagcataaa
    18361 caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag ctgaaaaaag
    18421 actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag acaaaacaaa
    18481 aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa gtagcaaaac
    18541 gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg attcgaagaa
    18601 tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt aggatctttt
    18661 gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg agtacttttt
    18721 aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca aaagcatgcg
    18781 atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct tgggaccagt
    18841 actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt ttagaagtag
    18901 gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga gcagtcagtg
    18961 gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc ttgcgaacga
    19021 tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg acggaaggtt
    19081 cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg ttgcaagctg
    19141 gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc caagcgttga
    19201 tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat ttttcaaaac
    19261 ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt tcgaaatcgg
    19321 ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac tgacaattgg
    19381 ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga aggacgtttt
    19441 atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag gctacaagag
    19501 tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac agatacgatt
    19561 cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa acaaaagctc
    19621 cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa cttccgagga
    19681 aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa gcggaatctt
    19741 tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg gttccaaatt
    19801 caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca gagaaaaaga
    19861 ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt atatattaat
    19921 tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat ttttagggag
    19981 agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta tttagggtgg
    20041 ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat tccgttgagt
    20101 ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt gcgtgctagt
    20161 ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc caccatggac
    20221 ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct ttttcatgcg
    20281 ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt tgatggagat
    20341 tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc gttgattgta
    20401 tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat tgcaccggta
    20461 ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg ctggctgccg
    20521 gaaactttac ttttgttgat agaagaggga ttaatcttat ttgagcgaat aatcgatttt
    20581 tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg gatgtatatt
    20641 ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca cttgttttat
    20701 gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc ttcaacgatc
    20761 gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt tcgaaaagaa
    20821 acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg ggcgcaaggt
    20881 atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca ggggataagt
    20941 gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga attacccacg
    21001 ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg acgaaatgag
    21061 ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat tgaaatcaaa
    21121 catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt tgaaaaggga
    21181 gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag gcgcttttta
    21241 tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta tcctaatctg
    21301 aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa cacagatttt
    21361 ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa tcgttatggt
    21421 cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac atggcgaacg
    21481 gatcaacaag gaatgatcac ctatcgctgg catccagttt ttcatcctca tgggctagtg
    21541 gaaacaatga tagactagta attctcatct gctcatggta ggataatttt tgaaagtggt
    21601 gagtaatttg agtatacaag cagagttaca aaaaatacgt aaagaaaaac ttgcatcttg
    21661 ttacttgatt ttaggaacag aaaagtttct ccaagatcaa gttcggacag aaattgtcaa
    21721 aaaaatccaa ttagagggcg aagatgactt aaatttttta agttttgaca tggaaaatgc
    21781 aagtttagat gaggtagtag ctgaagcaga gacgttgccg tttttcggag atcaacggtt
    21841 agtattcgta gagaatcctt attttttaac aggtgaaaaa gtaaacaatg gaatcgaaca
    21901 aaatactgat ttattgacgg cttatttgaa agaaccatta gaatcgactg tattagtatt
    21961 ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat tgaaaaaaac
    22021 agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat atctgatgaa
    22081 taccttagag aattcctcaa tcaaaatgga acgaagtgca attgatttat tcttgaggtt
    22141 gaccgactta gatttatcca aaatgatgag agagttacag aaattggtcc tttatggaca
    22201 aaatcaacag caaatcacgg taagagaagt agaagaatta gttcctaaaa cgttagaaca
    22261 taatattttt gacatgaccc aatatatttt aaaagggaag acggaacaag ctttgcgttt
    22321 atacgaagat ttagttatgc aaggggaaga aacaatcaaa attaatgcga ttttgctttc
    22381 tcaattacgt ttatttttac agacaaaatt tcttgtaaag attggttatc agcaggcaaa
    22441 tattgctgaa acattgaaaa tccatccata tcgtgtaaaa cttgccatgc aggaagtaaa
    22501 aaaattcgat gaacatttgc tggtacagtt gtttgatcaa ttagttgaga tggattatca
    22561 aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt tgcgcacagg
    22621 gcagttgtta agaaatcgat aagaagaaag acgagggcaa agcatgcggg aaggtcgttt
    22681 gtttttgtcc tttttttgac aaataaaaaa atcggccgaa gccgattctt ttatttagcg
    22741 atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat tagacctttt
    22801 gattcagcca tatcaatagc tttaacagct tctttatata acgcatctac gttgtcagca
    22861 cctgaagcta cagcatcttc aaattttttg attgcagtac gcatagcgct tgtttgagat
    22921 gaatttttca cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga ttcaatgttt
    22981 ggcattagtt tcacctccga taacttaagt tcttacgcgt gttaccgcat aattcaacat
    23041 tcattattat acctaaaggc agcgtgcttt gcaatatgaa catgacaagt ttttttcctt
    23101 aacagtcttt tattaggatt ttttttcagt aaaaacggat agtacaagct gaaaaaaatc
    23161 aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa ctagattttt atttatctat
    23221 ttaaacaaag tttgaatggg aatgtttggt actaagttat tatataacaa agagaaccgt
    23281 ttgcaaagtt ttttttgaaa agggatacga ttccctgttg ttctttgttt ttctattaag
    23341 aaactgcagt tattttataa ccagtatcct ttgatcagta aaggctattc tggtgtaccg
    23401 attattttaa tgtattcatc gatatttttt atcaacattt tttttatgac caactggttc
    23461 cgacggttcc atttgccact tacttccagc acatatttat ttaggggcaa acgtaagata
    23521 tattttgcca aatagcgatc tttaagcagg ccattgatca cacggtcctc aagctgcaag
    23581 ttgatcccag tcagcatcat tggctgttct tgtaaaacgt acgtatcaat taaaagacct
    23641 tcaaaagttt ccacaagcgt cttcctttct tttcaaagta acctgaaaac aggcactaat
    23701 tcttccattt tgatattcgt tgaagtggat gcgatcattc tttcttttgt ccactgtaat
    23761 ctagtataac aaaaaagagc agaagagcca aaaagaaaaa agagcatcga attctatagg
    23821 aagtctcatt ggcacggcct aaattattac aaaacattga ttaaaaaaag cccctgcatc
    23881 gcaggagcat cccctatatc gttttaatag gtcaatcaaa aagcagtatc aaacacattt
    23941 tgtatcaaat caggaaataa tagataagct aagcccagaa tcaaaagtac cgctagaaga
    24001 aaccagccga ttgttcctat gaaatacttt aatttttcga acattgtatc acctcgttgt
    24061 cagtatatca cggatcagta aattgggggt gttaagaatc tcctaaaata gacaaaattt
    24121 gttctatttt cctaaagcaa atacttgtaa gatacagtaa gtgataccag aagcaatgac
    24181 cggtccggcg gcgattcctc ttaagaagac aacacctaaa atcgtgccga aaacaagtgc
    24241 aacagtgatt tgtggatcag aagcaatcaa tcctacaccc ttggaagata agacagccac
    24301 tagcccaccg caaaaaacag ctacccagcc aataggggat ttaaaggctt ctaatagatc
    24361 acgtagaccg atttctcctg ttgcgatagg gattagaata gccgctgtga tgatcgtcac
    24421 cccaatattg attcctttgc cttgaagcca agggaaaaag gaacgggagg ctggtatgag
    24481 cttcataatc aaaacaaaag ctgccgctat catcagactt tgatttttag ctaaaattgc
    24541 tacaagtaaa ataattccta aaaaaagcca actttccatg aaatatattc ctcactttct
    24601 tcgaaacaat cataacatgt ttttagaaag ttacaaaagt aggaattcat ccttcaagac
    24661 ctattcataa gctgtttttt tcataaaata aacaaaatca taaaaaaaca ctttgcttat
    24721 ttgacagaag acagccgcgc tttgtatagt ggacaagact tacatttttt tgaagaatca
    24781 gaaagatcag gtaaaggtct tagtaaacgc aagccattca gaataaccaa tattgtgctt
    24841 ccttcgtgtc ctacaactcc aagaggtaga tttaaaaatt gcaggaagtt agaagcaatc
    24901 aacaagagaa taacaagaat cgaaaaaata atattttgtt tgatgatccg ccgcattttt
    24961 aaagatagcc gatgactgta gacaaattta tttaagtcgc ttttcattaa gacgatgtct
    25021 gcgatatcca tagcggcatc tgtcccttgc cccatagcga cacctaaaga agcggctgct
    25081 aaagcaggcg catcattgac tccatcacca accatcgcat ttacttggta aagttcattc
    25141 tcttttttga ctagttgtgt tttttcttcc ggagtacagc ttgcatagta atgttcaatc
    25201 cctacttgtt cagcaatggc ctttgctgtg ccagaatggt cgccggttat catacttgta
    25261 tggatgtttt gggatttaaa gtaggaaatt acttgttggg tatttgcttt tggaatatct
    25321 agcagaccta aaacagcaat taactgctga tctttagaaa gataaatcac ggttttgcct
    25381 tgattttcca aacgttcgat catttccgca atctctttgc tgattatcat cgtctcagga
    25441 tcatatgcgt gcttgccaac tttccattgc gcacctaaat aggtagtttg taatccaaaa
    25501 ccagtaattt cttctattgg taagtattgg atttcttcag gtattgtgca atcgaaatat
    25561 tgcaaaatcg catgagcaag tggatgcgtc gttttttgtt ccatagcaac aagaagttgt
    25621 tgtgccaaat ccttttcatc aaagaaaaag ctgtcagtta caactggttt tcctttagtg
    25681 agcgttcctg ttttatcaaa ggcaatcgct tttaattcag aaagcttttc taaatggata
    25741 ccgcctttga ttaaaatccc attttttgca gaatttgaaa gagcagctag agaagctggt
    25801 gtagcagatg cgacaagcgc acaaggagaa gccacaacta ataataccat tcctcgataa
    25861 aagctttcat cccaactcca acctaggaaa aagtggggga gaaggatcat tagaggaaca
    25921 gcaagcaaaa tcaattttac ataattattt tcgattcgtt cgataaatga agctgtttga
    25981 gttggcgtat tctgcgcttc ttctactaat tggataatct tagcaaagac agtttcggaa
    26041 ctcgtttttg ttactcgaat cgtgatggga tttccttgat tcagcgttcc tgcatacatc
    26101 tcatcgccta aatatttttc tacaggcaca gattcaccgc ttaatgcaga ctcatcgatc
    26161 gttgcctgtg tgctttctaa aaagccatct atcggaacag ctgctccttt agcgacaaat
    26221 accaagttat ccacctttag ttgagaaaca tctacttctt gcatttgtcc attttctttt
    26281 aataattggg ctttttgtgg ttgaagattc atcaaagcag taatttcttt ttgactttta
    26341 ttagttgtgt attcctctaa tgcaccgctt aaacaaaaga tgaatgtcaa cattgctcct
    26401 tcaaagtaat taccgatcaa acaggcacca atagcagcta aagccatcaa taaatccaca
    26461 ttcagtgtcc gatcattcca caattctttc aagccttccg atgtttgttt atatccaccg
    26521 atgataatgg cactgatata aaaaagtggg gtgatggtaa gagagaatcg gtcgaagata
    26581 aaaccgaaga tcataaataa aaggcagccg attgttgctg caaaagcttt tttctgatta
    26641 gtcatcgtta ttagacctcc ttgtcaaaac aaggatagca taataaaaat gtaattgata
    26701 ataataacag ctaaatgaga acgataatgg ttatcatttg gtgattaagc ttgatgttgt
    26761 ttttattatt tataatgatt attattaaag aaaataaaaa accagaacat ttctcaaata
    26821 agagaagtgt tctggttttt tctacattgc aataaaccaa taaccaatca aaatgatacc
    26881 aaggataata cgataccagc caaaagcggt aaagtcgttt ttcttcaaat agcttagtaa
    26941 gaatttgata gctaagacgg aaaccacaaa agatacgaat gttcctacaa gaagaacaac
    27001 agtttcttcc attttgaatg aattcccttc agctaaatat ttgacgatct tcaagccgct
    27061 tgcgccaaac atgacaggaa tccctaagaa aaatgaaaac tctgtagcga caaaacgcga
    27121 agcaccaatc aagatagatc ctaggatcgt tgcgccagaa cgggatgttc cgggaacaag
    27181 tgccaagact tggaataacc caacaagaag agctgcttgg tacgtaaagt cattcagaga
    27241 agtccaacga ggtgttttgt ttttgttgcg tttttcgaca ataataaagg caataccgta
    27301 aacgatcaaa acaatggcaa ctggtaagaa cttgtggaaa tttgcttcta accaatcatc
    27361 caaaggcaaa ccaatgatga tggctggtat gcaagcaacg atcactttaa accaaagtgt
    27421 ccacgtatct tttttttcta ccgttgattt acttggagaa aatgggttta acttgtgaaa
    27481 ataaagaacg acgacagcca agatcgcccc taattggata acgacattga acatcgacat
    27541 aaattgtgaa ctggcatcca gtttgatgaa ttcatctgct aggatcaaat gaccagtact
    27601 actgatgggc agccattccg ttattccttc aataataccg agaatcactg ctttgataat
    27661 atttgcaaaa aacataaatt ccttccttcc caattaagat acatcttata gtataccgtt
    27721 attttttgag aaacccaatc tgttttttaa tattaagaaa aacttgagaa ataaagcaga
    27781 taagtacacg aagagaaaaa agaagacaaa gccctcgttc agactttgcc ttttatgatt
    27841 ctctggtaaa aattaaaata tccccattat agaaacgagt cgcgactatg tgttttcccg
    27901 ttatcgatat aagacgtatc attgatgccg tggatagaaa atcgaccgtt tgcataagtg
    27961 acctttgtta cgctgccatt taaaagccca gcacgaacag gctgtttagg atcgatcaga
    28021 ttaagcaaaa aggcgatcgt caaaccatga gatacgacta aaacatttcc accaccattt
    28081 ttttcgcagt gatgggcgat atcttcaaat cctgtccata cacggtctcg caatgtttca
    28141 taagtttccg cccacccagc ggtatctaag ttgtatacag cattagcaat ttgttcaaaa
    28201 gaaacttctg tggtaaacat ttcatcatat gttttaaaat tcaatacacg agggatcaca
    28261 ccccacattt ccatgtcata tccaccttca agagacccga aacaccattc tctaatcctt
    28321 ttttcaataa aataaggaat ttcttttccc ttagaatgtt ctcctagaat aatacgggca
    28381 gtttcgattg cacgaccact gtcactagaa taagcaaatg aaaagtcgac attttttaac
    28441 ccgcgaccta aataatgaat gccttcttgt ccttgctttg tgaggggtgt atcacaccag
    28501 ccttgtactc gttcaattgt attgaacatc ggttttccgt gtcgtacgat ataaagtgta
    28561 gtttctgtca ttttattctc ctcctaagtc gttttaacga ttgaaaaaag gatttgtcct
    28621 tttttcctgt tcaatcgtcg ttgcatcgcc atgtcctgga tagactgcaa attcatcagg
    28681 taatacaaac agctgggtcc gaatactatg aagtaattgt tctaaattcc ctgtatacag
    28741 atcggttcta ccgatacttc ctctaaacag ggcgtcgcca gaaacgacaa aatcatcaaa
    28801 aataaagctg acgcttccta tagaatgccc tggggtaggg acgacgcgaa aagacatgtt
    28861 tcctaaagta taagttttca tttcaaattc atattctgca ggcgagacaa taacatcagc
    28921 aatatcatca tgtctaccga gtccagaaag attcaaaatc ggatcaccta gccatttttg
    28981 ctctagtggg ctgacataaa ctgggatttg ataatacgcg cgtaaaggtt ccactgcacc
    29041 gatatgatcg tagtgagtat gggtaagcaa aatcgctacc ggctgttggt ttgttttttt
    29101 gatttcttgt ttgattcgtt ccgcatcttc tccagggtca atgatcaaaa gtgcttcttc
    29161 gttgtagacc aaataacagt tttcttcaat cattcctgtt tttaatcttt cgatatgcat
    29221 gtgcattcct ccttgatagc caaaacttct ttcatccatt agtataacgc attcattgct
    29281 aggaaacaaa aacaggtact tatttctctt tacttatctg aattatcaga ctatatgaaa
    29341 agtatttgga tattctaaag agaaggaaag taagttttca tagcatttta atatgtcctt
    29401 gttacattag attcatccaa aaatccaaat tctatatcct atatccctgc ctaatcagca
    29461 gggtgttttt ttatgtttgg aaagagagct ctttttgcta aactaggttt tttcaggtaa
    29521 tcttagagag aaagaggtgg tggaaatgat caaacagtca aaaatatctg tgcggcactt
    29581 agtagaattt attttgcgac gtggaagtat cgacaatcgt aaaaaaagta accatactgc
    29641 attagaagga gcgagaatcc accgcaagct ccaaaaagat gcgggagaaa actatgaaaa
    29701 agaagtcttt ttaaaaacaa cagttcaatt agatgaatat tacctgatcg ttgaaggccg
    29761 tgcagacggt attttcaaaa gagacggtat ttattatata gatgaaatca aaacctccga
    29821 accacgattt gaagatttag aaccagaaca agtggaactt ttctttcatc aagcaagggt
    29881 gtatgcctat atctatagtc aagaacatga tttacaagaa ataaatctgc aattgactta
    29941 cttccagacg gcagaagaag tgatcacgag aaaggttgaa catcagacaa gagagcagtt
    30001 agaaactttt tttaaaaagc tgactgaaga ttataaagaa tggcttgttt ttcaagaaaa
    30061 ctggcgcact gtacgcaatg cctctttgat ggctttgaaa ttcccccatg atgagtatag
    30121 gaaaggtcag cgtgaactag cggtagctgc ttataaaacg atccgtacga aacagaaatt
    30181 gtttgtagaa gcacctaccg gaacaggaaa aacaatctct acgttattcc ccgctttgaa
    30241 agctgtaggg gaagaagagg gagaaaagat cttttatcta acggctaaaa caataactag
    30301 acaagtagct gaagatgcta tgacagcatt aaaagataca ggagctgaag taaaaagtgt
    30361 cactttgacg gcgaaagaca agatctgctt tttagatgag acgacctgta atccagatca
    30421 gtgcccatat gccaatggtt attacaatcg gattaatgaa ggtttatggg atcttttgaa
    30481 tcatgagaat cagatcactc gtgaagtcat cgaaacttat gcaagaaagc atacactttg
    30541 tcctttcgaa ctatcattag atgtcagtat ctggtgtgac gtaatcattg gggattacaa
    30601 ttatttattt gatccaactg tttatttaag acgttttttt gaagatgaaa aaaacgagga
    30661 ctatctgttt ttgattgacg aagcacataa tttggtgaat cgttcccgag agatgtattc
    30721 ggcagaacta tcttatgaaa aaacgaaacg aagtaaggaa gctataccaa aggaattcaa
    30781 aaaacttcat cgccgtttca acaaactatt aaaagaattt gattcgatcc gagaaatagc
    30841 aaaagaagat cattgggatt atcatcatca aaaagcaccg gctgaatctt tagtaaaggc
    30901 aggttatcaa ttaagcgaaa aaattaaaga atggctggca gaatttcctg agcacccatc
    30961 acaagaacaa ttgcttcctt attattttga tttgcttcat tttttgaagg tcagtgaatt
    31021 ttatgatgat cactacgaaa caacagtcga aaaaacgtat cgcgatctga tagtaaaaga
    31081 gttttgtatt gatccaagct tgtttcttga gcagtcgttg gataaaggaa aaagcagtat
    31141 tcttttttct gctagttttt caccactctc ttattatcag gaaacactgg ggggacaaaa
    31201 aagtctggct tacaaactgc cgagtccttt cccagaagga aaccagcaag ttttcatcgc
    31261 aaattaccta gagacgactt atcgaaaaag ggaacacagt ctggcaaaat tagtagagac
    31321 gattcatgct tttgctgatg gaaaggtcgg gaattatttc gttttctttc cttcttatca
    31381 atatttagat cttgcggtgg aagcattcag acagcaatat cccgacagca atgtgctaat
    31441 ccaagaaacg gacatgaatg aggaagaacg cgaacgattt ttaacaaaat ttcaaacaaa
    31501 tcccgctgaa acattgattg gattttgcgt acttggcggg attttttcag aaggaatcga
    31561 tttgagaggg gaccgattga tcggcagtat gatcgtaggt gtaggtttgc cgcagatgaa
    31621 tcatgagcag gaactgatca aatcatatta tgatgagaag gaacaccttg gatttgctta
    31681 cgcttatcag cttcctggaa tgaacaaagt cttgcaagct ggtggtcgag tgattcgtgg
    31741 catggcggat caaggaatcg ttgtattggc agatcaacga ttttctagtt tttcttatcg
    31801 acgtttattc ccacaacatt ggcaaacagc aagagaagta cgaagtgttg gggaattgga
    31861 agaaggaata cagcgttttt ggttatcaaa agaagcagta aatcaaaaaa acgaggaatg
    31921 acagacgtca gcctcgtttt ttgattaacg atagttagtg aattgcaact cgattggtaa
    31981 gtcaagttta cgtaataaag caatcacagc ttgtagatca tcacggttct tacctgtcac
    32041 tctaatctga tcttcttgga tttgtgcttt tactttcagt ttttcatttt tgatagctgt
    32101 agtgattttc ttcgcgtttt cacgatcgat tccgttgacc aaatcggcag tttgtcgagc
    32161 tttgccacct agagcgtgtt ctgattcgga aaaatggata tttttgattg gcacatttct
    32221 tttgatcagc ttaccaaata atacatcttt gatctgttcg attttgaaat catcatcgct
    32281 caatacaact agattgctgt tctctaattt gatttctact gtagaatcct tgaagtcaaa
    32341 gcgattggac aattcttttg ttgtttgctg aatcgcattt tttacttctt ctgtattgac
    32401 ttctgataca atgtcgaagc tagcatcttt tgccatagga accctcctga tatacatata
    32461 aatatttacc atacatctat cttatcatag gattagggca gattcttacc tgtttgctaa
    32521 aattcttctc atttgagttc gaagtatttt attttaatag ataaactgtt attcttacat
    32581 ttggaatacc aaagtagggg agaggtgctt gtgagtaaaa ttatgactgc gtatcataaa
    32641 aatgaaagat taaagaaaat agtggttatt ttgataaccg gattgaccgc tgcggtcgct
    32701 ttaaattatt ttttgattcc agccaatgtc ttttctgcag ggatgaatgg gatcgctcaa
    32761 atcatttcct cgcttttata tgaatggttt catatcaaga tagacacagg aacatttatt
    32821 tttcttttga atatccctgt gtttgtatta gggtttctca aagtaggaaa acgagcaacg
    32881 atccttagtt ttgtgaatgt tgtatgtgta tcggtgatga cgatgctgct tccagtcgga
    32941 cagatcactg ataatatttt gatgaatgcc ctagtaggcg gtgttttatt aggtcttggc
    33001 gtaggattat ctttaaaaat gggctttaca actggtggga tggatatcat ctctcttgta
    33061 ctttcacaaa caactggaaa aacagtcgga aactacatgc tgattctaaa tggtatcatt
    33121 gtagtcatcg caggcttctt ctttaactgg gaaagtgcct tgtatacgat tatttccatt
    33181 tatgcaatga ctcaagtaat tgatgccatc cacacgagtc atcaaaaagt aacagcaatg
    33241 atcgttaccg tcaagccaga agaggtaaca gaagaactag caaaacggat ggtacgtgga
    33301 atgacattac tgccgtctgt cggtggctat tcaaaaaaac aagggaacat gatcatgatg
    33361 gtcatcaccc gctacgaaat gtatgatctt gatcagatcg ttcatgaaat cgacgacgat
    33421 gcatttatca atattttacc aactcattca gtatttggcc ggtttgcaaa tgaaaacgaa
    33481 caaaaaatgt atcgaacaac tggcgtattg ccagaaataa aaaatcgttc acaaaaaaaa
    33541 tgaggaatca ggtttatttt agaataaatt taatctatta catctaataa gaacattttt
    33601 ataaatttaa acgagaatct tacttctaaa ttctcattta caagcagcta aaaaaagagt
    33661 atgataaata aaaatcgtgt taaggagtgt cgtacgatga gcgaagcaaa ccaagaacgg
    33721 tctggacaag aaatagaact aatcaaagaa cgtattcttg ctgccttgga aatggtgatt
    33781 gatcctgaat taggaatcga catcgtcaat ttaggcttga tttatgatat agaattcaat
    33841 cctgaaaatg gagaaacagt cattaagatg actctaacaa ctatgggctg tccattagca
    33901 gatattttaa cagaatctat ccatgacgca ttaaaagaag tgccagaagt aacgaaagca
    33961 gaagtcaaac tagtctggta tccagcatgg acgacagata agatgagccg ttatgcacgg
    34021 attgcgttgg ggatacgata gaaatcttgg ctgactagct atgagaagca aataggaaaa
    34081 acaaaaaagc cttttatcct ttctagaaga aaagagataa aaggtttctt tgtttataaa
    34141 attttgtctg ctatattata agttaattaa ataaatgtgt tctattgata aaggacattc
    34201 aaatcccttt ccttttggaa tccttctttt ttacatcagt ttgattttcg tatagtattc
    34261 tttctcatct tcacgataaa tcgcagcccc atttttttgt tgtgctttaa gactgccaag
    34321 atttttttta tttacagaca aaagtgcctc atcaatgccg attttttttg cctcttgtag
    34381 tactaatgct aatccttgtg tggcatatcc ttttttacga taattagggg agatgccgta
    34441 accaatatgg ccagcgccgt ttcttaatgt ttcgttcagt ttatggcgca aattaaaaat
    34501 tcctacatac tcatgttcat cactttctaa aataaagacg gttgcgggaa cgtaactctc
    34561 aggaagatcg attcctaaag acgttttttc gcattttttt acataatcta aaaattcagc
    34621 aaatgtatag ccataagcat tgttgataaa tccactctca tttggtccaa agctagtttg
    34681 tagctgataa gctttcttta aatctgcttc ccatagtttg atcagtttca tcgtattcac
    34741 ctctttaagt aaccttattc atttgttttc tactatagca ctattgttgt aaatagccat
    34801 gcctattttt atttattgtg tcatatcgta ttaagaatca tcgaacaata caaatatgaa
    34861 ttacaataaa aaaattattt gaagttcgtt cattcgttac ttatgatctc tagaagaaag
    34921 aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat agtaatcaac agaagaaata
    34981 atcaatatcc aaatgaaaga agatagctga atcgagaaaa agaggaaaaa agaaatgaag
    35041 aaaaaaataa aatagttact aatcccagag tcgttcgttt aacattgaaa tcgatcatat
    35101 ggataaaaca gattttttca ccaaccctca atagatgatg gagttgctcg gatgaattat
    35161 ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa agaacaaaaa tatgttcttg
    35221 acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc ggctatggcg gaattggcag
    35281 acgcgctagc ttcaggcgct agtgagggtg acctcgtgga agttcgagtc ttcttagccg
    35341 cataatttgt ttttttataa agagaaggta cacctggagc gatgagaaag aaaataacat
    35401 attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa ataaatcaaa
    35461 aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag attgattcaa atagtagatt
    35521 tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag actaaactta
    35581 aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat attttgaatg
    35641 tgtttaaacg ttcaaataat gaatcttagt accaaccgtt tgcttcccag aatgctttcg
    35701 ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt tcttggtttg
    35761 cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacggccg atgtaacgtc
    35821 cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct tttgcagagc
    35881 ttgtacttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt gtttcagcaa
    35941 caggcgctgc ttgtgtttcc gtttgaaccg tttcttgtac aacaggttgt tcagcttgaa
    36001 caggttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca gctgcttgtg
    36061 cagagccttc tgttgggatt gttaattgtt gtcctgagta gatcaggtta atatcagaaa
    36121 ttgaatttga ttctgcaatg gcattgatca aggagttatc accaacgtat ttttgagaaa
    36181 tcgttgatag agtatcacct gattgaacgg tataagcttc gtccgcatga gcagtcgttc
    36241 ccatgaagaa tgcagcgccg gcagccaaag ttgttccaaa aagtaaagtt ttaagtgatg
    36301 tcatgaaaaa tctcctttat tcgttaaaaa gttttatttg ttgtgttcaa cgaggaatac
    36361 tttaacacgt gaaaatattt cataggtaaa agaagcatga caatttatga caaatccacg
    36421 ttttttgtaa cagaatgatg ttcgctcgat gtgaaaaatg atgaggaatt tcacataatg
    36481 cagaaataat gggattagaa caggtgttct gatgagaaaa gaaatgtata aataacttga
    36541 aagaaattgc ttttgttcat cattttattt acatattgcg tatatttttt gtatttttgc
    36601 ttgagatgtt acaataaaag aaaaaggagg cggttccaat gagtaaaaga taccagaaca
    36661 tcatggtagc agttgacgga tcaagacaat cgatcaaagc gttcgctgaa gcccttgact
    36721 tagcaaagga caatgaagcc cacttattta tcgtgtcaat catcaacaaa gttgagctga
    36781 cccatagtgc ctatgccttt tcaaaaatct atgcagatga aaaacaaaga acagaagtag
    36841 ccatgctgaa aaaaatcaat gatgccaaag agtacggcat tgctgagatc cacgcaatag
    36901 ttgaaactgg agatccaaga aatttgattg ccaatgtcat ccctggacaa gaaaatattg
    36961 atttgatcgt tatgggagcg acaggaaaag gtgctataca acaagcatta gttggatcga
    37021 ccgcttctta tgtcgtatca catgcccctt gcagtgtgtt ggtcgtgaag taatgagata
    37081 agggaggaat agaaaatggt tcgtcctcaa gaagtccgtg cgccaaaaga aaaaatagaa
    37141 attttagcaa ttattgaaga cggcacacaa acaaagaagg gatactcgat cgctttagta
    37201 aagtggaaag gaaaaaaagg tgttgctctg agatgggatg gagataacca acaagataaa
    37261 ggattccctg tcacagctaa cggttatcat ccagcgtggt ttgttctgcc tgataaattt
    37321 atggaattat acagctatga ttatgcaaga gccgcagcat caatcaaagc attagataaa
    37381 ttaacagaag agcagaatga aatggaggaa aaataaaaaa gacgaaccaa tcagccttta
    37441 gaaaactgat tagttcgagg agtaaaaatg aaaaaaggtt atttgggatg aaactagaat
    37501 aatgcgtata acttaaataa aacttaaagc aataaaatgg caagagggtg aggcaaatgg
    37561 aagcaggaaa agtattacag gaagtaaaag cactatgtgc agataaaaaa tatgaagaag
    37621 cgaaaagcct gatcgaaaag aacaaagaaa atttggaaga aaagtacccg atcgcacaag
    37681 aatttataga attaaaacaa ggaacgatcc ttcaccgatt caaacatttt tttggtataa
    37741 cagaatagaa aaaacgatta aagatcggca gaaatttgct gcatctttaa tcgttttctt
    37801 tttaaacatg cgagggattc aattctgatt cttatataaa aaatagatcc aaataataag
    37861 aaaaattact gaaagcgtca attcgatgat gtgtgttatt aatacagacg ttgtttccca
    37921 atgcctaaaa gaaaaaattt gataagtaaa atatacagca ggtaaagagg ccagtaaaac
    37981 tgaaaacttc ttttttgtcc attttatgta atagaaaaga atgcttatca taataaaaaa
    38041 aggaatataa aaaacaaatt gaccgatcgc gatcatacta ttgcctccga taaataaagt
    38101 ccatgataca aaataacact ttgtgcgaaa tggcgcaagc agttttgtgg atttatccgg
    38161 taaaatctgt atcgattttg aaattagagg agaaacttgc tataatcaaa gtgaaagaag
    38221 gagtgggatt ttcatggatc atgtagtgtt agaaatcgat ttgcatttga tcaataatat
    38281 tcgggtgatt tactatgttg taagtaacag tgtcgaacaa cgtgtactga cgaataaaat
    38341 aaatggtatt ttagcaaaga aagacgttca ccgattcaat aatggtgaag gaagttatta
    38401 tagtatccca gtagaaaaaa ttatttatac gacagtcaaa gtcagagaag atctagatac
    38461 caaaaaagca tatgaaccta tttttacaac ttattgattg acagcagatg gaggaaaaaa
    38521 atgaatccat atgaacaact tattcaagat ttgatcgatg gaaaaatcga aaagattgat
    38581 gtaaaaagag aagaattaat ggcttttcga gaagcgtggc tgaagttgga gaatcgtaaa
    38641 tatgtggtgg gaaaagcctc actgaacggc aatgtgactt atcattacga tccgacacgt
    38701 ttattttagt atgaagtatt cagaaaagaa ccaaaggagg acgatgcagc agcattgtcc
    38761 tcctttgatt ttacaaaagt gaaagtttta cttatttgaa tagattttta aatttgtttt
    38821 tgacactgtc gatatttaca tcttttaaaa gattttgggc tttctcttta taaggtccta
    38881 attgttcttt atgttcttcg ataaatcgtt ttgcgccatc gaaattgccg ttcttaatca
    38941 tttccttgac tttttgaaat aattcttctt gattcatttt gaacacatcc tttgctatca
    39001 acatgatttt ttacatggac aaaacgagaa aaactgattt atccacacgt caataatatc
    39061 aaataacgat catttttact aaaaaaacac tcgattctcg cttctaaagt aggagaaaaa
    39121 tctccaactt gggacggatg gaatttcttt aactgaccca tatactgaat atataacaag
    39181 gaggtagaaa gatgaaagtg attaaatatt tgattggact ggcggcgatt agtggtattt
    39241 tgctatttgg actgaaatat tatactgaag gaagttatgg agaaatccct ggtatacttg
    39301 atcagctaaa tccattagtt gaaaaaggag aggtctatgt aaagacacaa aaaccagagg
    39361 aagtaaatga atatggtaca gcaaggtatg tgcagaaagc tgctgatgcc aatgggaaag
    39421 aacgaacggt tgaatttaat ggtctttctg tgttaaaaga aaatcattat ttgaaaatta
    39481 gcaataaagg agcacacata gaaacgtatg aggaagttcc tagagaaaaa gttccggaga
    39541 aagcattagc aattattggg taaatcagtt tttgtattaa gaatacgccc gattttatta
    39601 ttcataattt aattatgtaa actaaataca agtagtaaaa ataatacaat aatccccctg
    39661 caaaaagatt gttcttttac agggggttca ttttttcagc aagcacatca aattgggatg
    39721 gcagggttcg aacctacaat tgcttgattc agagtcaagt gccttaccag tttggctaca
    39781 tcccatttat tcaacaaaat aatcatagca ctttcctaaa aaaacgcaag ttatttttct
    39841 tgatcggata gttgttgctt tactcgtaaa aggcggcgta ctagtttctt ttctaatcca
    39901 gcaggaaggg caatcagttc tgctactgac aagacggtgt ccaataactg atattctttg
    39961 acaggttcat tcataatatg atagatgttt gctaaacgtt caatatattc aaagcgagta
    40021 tactctgttc gagcaattag aaatttcaat aaatcggtgg ctttctcgta ctcttgatct
    40081 gcgtaataaa caagagattg acgatacaat ttgatttcca gttcattgag ggtagcaggt
    40141 ctgcgacgct cgttccactc attaatagag actttacctt gaacatttcc cttgcagtca
    40201 cggacaaaaa taatcggaat ttcatttgga tccatttttt tatgtgtgaa tttttccatt
    40261 atctacgctc ccttcaaagg tttggtatct tgagtataag ggatttttgt ttgaattact
    40321 agcttttctc caaagttagc atctgtccac ccattgcagt gaaaatcgcc gaaaaatcgt
    40381 tgatcggtac tgctcgattt tttacaccat agggatcgtt gacataaata ttttctttgt
    40441 catatccagt gactacacaa gcatgaacaa gaggagtgac tcttacttct ccattatttg
    40501 tggtccaagt cataaaatca gtttcatctg ggacttcaaa attgatagtc acagaagtcc
    40561 agacaggatt tcctttagat agtgcttttt tgatatcatc gagttgagca tcggtttttg
    40621 gaaaagtgtg ataatccgtt tggacgattt gctgagcaag ggaggcaact gggggaacat
    40681 agacgaccat agcccagtct ccgccactga tatcaccaac aaatccttca ttaggatcgc
    40741 catgagtccc atctgcattg aaaacaggga cgtaatccaa ttgctcagct aactggtttt
    40801 tagttgtttc ataaccataa aaagataaaa gcatagaaag cgaagtgact tcacagccat
    40861 tttctaaaga aggagaatca aactggctct ctaatggaac atctagtaaa acttctgctt
    40921 tctcttttgt ctttttcgtt ttttgtgttg aacttttttg tgcagtgttc attgtatgtt
    40981 ctggagaatg gatatgtagg taataacttg taccgcttaa taaaagagca gctgtgcccc
    41041 ctagaaacaa tagttttttg aacgtttttt tcttcatttg aaatctccct tttcagctgt
    41101 ttatctttcc tattataaat gtatttttat tggaaatgga agtattatat tttaatatgt
    41161 aggaaataat tagtttttaa ggaatatgtt ttaatttttt gaaaaaaatt cttctaaata
    41221 caacagctgt tttaattgtg aaaacgaaaa cagtacgcta taatgcctgt aaggttgatt
    41281 tttaactgta aataaaaatc aatgagttaa atctccaaag aaatagcagt ggtaatgtgg
    41341 agaaaagttc cagcagttta ggaggatttt gatgacggaa gaaatgatta atttaggaga
    41401 acaatatgca tgcaaaccaa ttggttttac aaaaacggtc attggtgaag ttgtatcaaa
    41461 aatgacaaac tgcgccgtgg tcaaagttgc ccaatgtgct gcagaggacc aagaattatt
    41521 agatgaaaaa gcaagcatgg ttgttgcaaa atatgataca tttgaataaa gaaaacaatt
    41581 ggacgtgcgg cgtgctgcaa gtccaatttc tttgagaagg taaatcagta ttcttttcct
    41641 attttctgga gaagaaaaag cgaggcgagt tgtcatggta acattcttta cggttctatt
    41701 gtttttattc gttgttgtga tgtttttatt tctttggctg gttcgaaaag aaattattta
    41761 tcggactgtg cgaaatcgat gggtctactt ggtgataccc ttcttagtgt cactagtcat
    41821 ttggtataca ctcatcagtc atcctacagc agatgaattg gcaaaaggga tcttgtcagc
    41881 aatgattttt attagttttt tgttggacag tagaggaatc acagaagaag gacttgtttt
    41941 gaatagcttt gacaaaaaag gtgttccttt ttctgagatc aataaaattg tattatatca
    42001 gccaaaaggt tctaaaatcg taaaaatgaa ttttttccga aacggatggc gaggaccgat
    42061 gctgaaattt tctgcatcat tagaagaatt agttccattt ttgtcccaac gcttaaacga
    42121 tgaggcagag attgatatta tgattgatcc agaataaaaa cagaggttgc gacaagatgt
    42181 ttgtcacaac ctctgttttt tatttcaaat aaaattatcc aaatacgcgg aagcgatcgg
    42241 catcgttcat gtattctttt tccccagccg gtgtttctgg tgaaccgaat actaattgcg
    42301 aacgtagttt ccagtttgaa gggatattcc attcttttgc aactgcttcg tcaataactg
    42361 ggttgtaatg ttgcaagttt gcgcccaatc ctttatcaac taatgctacc caagtattag
    42421 ctgttgcgat tccgtctgat tgttctgacc aatctgggaa gttgtctgca tataattcaa
    42481 attgttcttg tagacctttt accacgtcag tatctttgaa gaataatact gtaccataac
    42541 catttttgaa accagctaat ttgttttgtg tatttgggaa agcttctgct ggagtcaatg
    42601 ggcgaagtgc ttcttcagtg atttcccaaa gtttttcatg tgcatctccg aaaaggataa
    42661 ctgcgcgtgt tgattgagca ttgaaagcag ttgggctttc tttaattgct tctttgatca
    42721 aagttgttaa ttcttcatta gaaagtgaaa cattacgtcc tagatgatag attgaacggc
    42781 ggtttttcaa agtgtcagta aaagttgtca taataaaatt tctcctttga tttgcgtttt
    42841 gcattttttt aattgattac gaaatgaagt atagcacctt acaaaaagta agtaaacaga
    42901 tatgctcaca aaaaataagc gaagttcaaa aaacaatttt aataagaaaa atcgttatgt
    42961 aaggattttc ttagttatcg ctttcattgt tgtgttttag gaatttttgc ttatttgaaa
    43021 aaaacagctg atatataatt attaaatctc tatcttcttt tttttatgat attgccatta
    43081 aaaattactt gcttttatag aaaattagtt tatatttctt aaataagttt tttagttaaa
    43141 tttttcggta aagatgtata atattttctg aaaatttaaa gaattggggt atagattatg
    43201 gaaaaacagc aagacaaggg cttcttcggg cagccgaagg gattgtcaac tttgttcttc
    43261 actgagatgt gggaacgatt tagttattac ggtatgcgtg cattacttat ctattatatg
    43321 tacgatacag ttgccaacgg gggacttgga ttaccacgag caacagcatt agctatcatg
    43381 tcgatctacg gatctttgat ctacatgtct agtatcgtcg gcggatgggt atctgaccga
    43441 gtgttaggag caaaacgcac tgtattcttc ggtggagttt tcattatgct tggccacatc
    43501 gtcttggctt taccatttgg tgtaagtaca ttgtttgttt cgatggcatt gattattatc
    43561 ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg gacatcttta ttcaaagact
    43621 gacttgcgaa gagatgcagg attttcgatt ttttacatgg gtgtcaactt aggggcatta
    43681 ttagcaccac taatcgttgg tacaatcggt caagaatata actatcattt agggttctct
    43741 ttagctgcta ttgggatgtt ctttggatta ttacaataca tcatccaagg acgcaagaca
    43801 ttagacggag tgggtacaac accgccgaat cctttgacaa ctgaagaacg taaaggattc
    43861 atccgtaact tgatcatcgc acttgttgtt atcgctgcag tattcggcgg ggcacaacta
    43921 actggtcatc tgacaattga cttttttgtc aatacaatca gtgtattggg gattatctta
    43981 ccgttgtatt actttatcaa aatgctgact tcaaaagatg taactgctga agaaaaacca
    44041 agagtatggg cttatatccc attattcctt gcagcaatca ttttctggtc attacaagaa
    44101 caaggttcat ctatcttagc tttatttgct agtgaacgta cacaatctac gctatttggt
    44161 gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa ttttcatctt tattctgaca
    44221 ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc aaccatctac agtagtgaaa
    44281 ttctctcttg gacttttatt tgcaggatta tcgtacttgc tattgatgtt gccgggtatg
    44341 ctttatggta caagtggaaa agtaagtcca ttgtggttgt tgggaagttt cttcatcgtg
    44401 gttattgctg aattgtgtct ttcaccagtt ggtttgtctg tgacaacaaa attagcacca
    44461 cgagcatttg aatcacaaac aatcgctatt tggttcttag ctgatgcttc ttcacaagca
    44521 atcaatgcac aaattgcacg cttctacaca ccgggaacag aatctgctta cttcggtatt
    44581 atcggtgcag tagcaatcgt cggtggtatc ttgctattta ctgtgaaaga accaatcaaa
    44641 aaactaatgg gctctattca ttaatcaatg aacataaaaa caggaattgc ttatttgcag
    44701 cagttcctgt t
//
LOCUS       DACEKG010000014        44658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEKG010000014 DACEKG010000000
VERSION     DACEKG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.014"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(261..1058)
                     /locus_tag="IW664_000702"
     CDS             complement(261..1058)
                     /locus_tag="IW664_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0848086.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1055..2239)
                     /locus_tag="IW664_000703"
     CDS             complement(1055..2239)
                     /locus_tag="IW664_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0848087.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2264..3511)
                     /locus_tag="IW664_000704"
     CDS             complement(2264..3511)
                     /locus_tag="IW664_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0848088.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3726..5471
                     /gene="dnaX"
                     /locus_tag="IW664_000705"
     CDS             3726..5471
                     /gene="dnaX"
                     /locus_tag="IW664_000705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0848089.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5491..5805
                     /locus_tag="IW664_000706"
     CDS             5491..5805
                     /locus_tag="IW664_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0848090.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5830..6426
                     /gene="recR"
                     /locus_tag="IW664_000707"
     CDS             5830..6426
                     /gene="recR"
                     /locus_tag="IW664_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0848091.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6490..7134
                     /locus_tag="IW664_000708"
     CDS             6490..7134
                     /locus_tag="IW664_000708"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0848092.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7149..7478
                     /locus_tag="IW664_000709"
     CDS             7149..7478
                     /locus_tag="IW664_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848093.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7492..8430
                     /gene="holB"
                     /locus_tag="IW664_000710"
     CDS             7492..8430
                     /gene="holB"
                     /locus_tag="IW664_000710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0848094.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8466..9290
                     /locus_tag="IW664_000711"
     CDS             8466..9290
                     /locus_tag="IW664_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0848095.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9283..9630
                     /locus_tag="IW664_000712"
     CDS             9283..9630
                     /locus_tag="IW664_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0848096.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9699..10571
                     /gene="rsmI"
                     /locus_tag="IW664_000713"
     CDS             9699..10571
                     /gene="rsmI"
                     /locus_tag="IW664_000713"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0848097.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10680..11801
                     /gene="dinB"
                     /locus_tag="IW664_000714"
     CDS             10680..11801
                     /gene="dinB"
                     /locus_tag="IW664_000714"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0848098.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11855..12457)
                     /gene="nrdG"
                     /locus_tag="IW664_000715"
     CDS             complement(11855..12457)
                     /gene="nrdG"
                     /locus_tag="IW664_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0848099.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12772..14961)
                     /gene="nrdD"
                     /locus_tag="IW664_000716"
     CDS             complement(12772..14961)
                     /gene="nrdD"
                     /locus_tag="IW664_000716"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0848100.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15287..16054
                     /locus_tag="IW664_000717"
     CDS             15287..16054
                     /locus_tag="IW664_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848101.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16044..18086
                     /locus_tag="IW664_000718"
     CDS             16044..18086
                     /locus_tag="IW664_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848102.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18367..18516
                     /locus_tag="IW664_000719"
     CDS             18367..18516
                     /locus_tag="IW664_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0848103.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18527..20041
                     /gene="dltA"
                     /locus_tag="IW664_000720"
     CDS             18527..20041
                     /gene="dltA"
                     /locus_tag="IW664_000720"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0848104.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20038..21243
                     /gene="dltB"
                     /locus_tag="IW664_000721"
     CDS             20038..21243
                     /gene="dltB"
                     /locus_tag="IW664_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0848105.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21288..21521
                     /gene="dltC"
                     /locus_tag="IW664_000722"
     CDS             21288..21521
                     /gene="dltC"
                     /locus_tag="IW664_000722"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0848106.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21524..22792
                     /gene="dltD"
                     /locus_tag="IW664_000723"
     CDS             21524..22792
                     /gene="dltD"
                     /locus_tag="IW664_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0848107.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22933..24015
                     /locus_tag="IW664_000724"
     CDS             22933..24015
                     /locus_tag="IW664_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0848108.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24015..24818
                     /locus_tag="IW664_000725"
     CDS             24015..24818
                     /locus_tag="IW664_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0848109.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24888..25628
                     /locus_tag="IW664_000726"
     CDS             24888..25628
                     /locus_tag="IW664_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0848110.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25639..26643
                     /locus_tag="IW664_000727"
     CDS             25639..26643
                     /locus_tag="IW664_000727"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0848111.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26688..28532)
                     /locus_tag="IW664_000728"
     CDS             complement(26688..28532)
                     /locus_tag="IW664_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848112.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28744..29262
                     /locus_tag="IW664_000729"
     CDS             28744..29262
                     /locus_tag="IW664_000729"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0848113.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29390..30073
                     /locus_tag="IW664_000730"
     CDS             29390..30073
                     /locus_tag="IW664_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0848114.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30382..30455
                     /locus_tag="IW664_000731"
     tRNA            30382..30455
                     /locus_tag="IW664_000731"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30416..30418,aa:Arg,seq:tct)
     gene            30614..30973
                     /locus_tag="IW664_000732"
     CDS             30614..30973
                     /locus_tag="IW664_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0848115.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31096..32727
                     /locus_tag="IW664_000733"
     CDS             31096..32727
                     /locus_tag="IW664_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0848116.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32789..33430)
                     /locus_tag="IW664_000734"
     CDS             complement(32789..33430)
                     /locus_tag="IW664_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0848117.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33712..34020
                     /gene="rplU"
                     /locus_tag="IW664_000735"
     CDS             33712..34020
                     /gene="rplU"
                     /locus_tag="IW664_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0848118.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34047..34391
                     /locus_tag="IW664_000736"
     CDS             34047..34391
                     /locus_tag="IW664_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0848119.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34404..34697
                     /gene="rpmA"
                     /locus_tag="IW664_000737"
     CDS             34404..34697
                     /gene="rpmA"
                     /locus_tag="IW664_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0848120.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35104..37938
                     /locus_tag="IW664_000738"
     CDS             35104..37938
                     /locus_tag="IW664_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0848121.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38129..38620
                     /locus_tag="IW664_000739"
     CDS             38129..38620
                     /locus_tag="IW664_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0848122.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38723..39712
                     /gene="manX"
                     /locus_tag="IW664_000740"
     CDS             38723..39712
                     /gene="manX"
                     /locus_tag="IW664_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0848123.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39745..40548
                     /locus_tag="IW664_000741"
     CDS             39745..40548
                     /locus_tag="IW664_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0848124.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40569..41483
                     /locus_tag="IW664_000742"
     CDS             40569..41483
                     /locus_tag="IW664_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0848125.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41566..41940
                     /locus_tag="IW664_000743"
     CDS             41566..41940
                     /locus_tag="IW664_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0848126.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42079..42324
                     /locus_tag="IW664_000744"
     CDS             42079..42324
                     /locus_tag="IW664_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0848127.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42496..43560
                     /locus_tag="IW664_000745"
     CDS             42496..43560
                     /locus_tag="IW664_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0848128.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43806..44231
                     /locus_tag="IW664_000746"
     CDS             43806..44231
                     /locus_tag="IW664_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0848129.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44224..>44658
                     /gene="nusB"
                     /locus_tag="IW664_000747"
     CDS             44224..>44658
                     /gene="nusB"
                     /locus_tag="IW664_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0848130.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKEL"
BASE COUNT        14181 a         7694 c         9376 g        13407 t
ORIGIN      
        1 acagcttttc atgtttgatg aatattgtga cttgtgaccg aggagttggt aggagaacac
       61 cgtttaccaa gaggttgtga aaaagctgtt ctgcatcaag taataagaag agccaagtaa
      121 aaacagtctc ttatgttttt aacttggctg gacttatcac cgtagatgta agcttttgaa
      181 cgccgtttat ttgcaacaaa aggggctgtg acacgatttt tgtcacagcc ctttttttgc
      241 gaacattcat ttagcaattt ttagaaactt ctgccgccac cgccaaaagt cccgccaccg
      301 ctagaatggg tagtacttcc gccaccgccg cttccaggag gtgggctttt ggggattctt
      361 cttgtcgtga caaaagaatt tgtcagacga tcggtttttt cagttaattt gatactggat
      421 ttttctcgga atggatattt gtagatgcct gatttcaatt gataacgcga aacagtcaca
      481 aaataaaaga caagactaag gataagagcc aaaacaacag caatgatgat ttctactgtc
      541 gtcaaaacct tatagcgtgt aattttgcca gtttcttcgt ctacacgata atgtcctcct
      601 ggaactccgt cttccacata tgttgctgat ttagacaggt aagcttttgc agcattgtaa
      661 taggcagcat ttgtcatctg atcataaaca tcatctaaga tcgaatcgat tctgctatca
      721 gtcaaataat cgatcatatt accagaagtc gaaatgtaga tttcacgctg tcccatatct
      781 aatagcaata ctgagccatt ttgatcattt ccgactgcgt ctcttaagta attatcagca
      841 aagtctcttg gttcttctga attagatgta gtcgttacga tcaagactcg tcctttgatt
      901 ttttcattca gcttttctgc ctgttgttcc aaagattgga tttgatctgt tgtgaataac
      961 ccagcctcat catttaccgt agctgtatct gcatgagtag ccaccggcca gccaacactt
     1021 actagcaaaa gaaagaataa aaaaaatttt ttcatcatat caagtaacct ccgatcagaa
     1081 agaggatcgc tagcccagcg aaaatcccaa gagcggtcag tcccaatttt ttctggctga
     1141 tcggcaggac accgctgacc tttcctgttt gtccattcat tgcgtagtag tagacttttt
     1201 tacttgagtc attgcttcga taagtaacaa gccataccgg cagtaatacg taattattta
     1261 cttcgtttgt gattgaagca gaagttcgca caccggtcaa tgttgtatat ccatttgcgg
     1321 tatcttttaa cagcttctct gaatagtctt ttaattcaga ttgaatctgt gatttgattg
     1381 cctcatattc gatatctctt ttttctgctt ggaacccggc taaatactgg cttttaaaat
     1441 caattgcttt atctaacggg aaaggttgca cgccttcgac catcttttgt tgcgtatttt
     1501 ttgataaggc attttttact agttctttaa aagaaagctt gcctgctctt gaaacagcaa
     1561 attgcttcgt ttctgtgtat tcgatgtcac ctatccgcca tatgcggata actgtaccat
     1621 tcgcctgcat ggcaccatct acttcagcat ctgtagccca ataagggaaa tacacacctg
     1681 tcaacttatc gatttgatcc ttattgaaaa aagctttggg aacaaaccat tttttcttgg
     1741 tccaagcgag aaatctgttg attgcttctt ctttctcgac agcaaaagga agtactttat
     1801 ttggcaagaa ttctccgctt aaacgtcccg atagtacgac gggattatgg cagtagtaac
     1861 aataagtcgc agcagttgtc gcatcagtca cgatctctgc accacagttg ggacaaagaa
     1921 aaagatctat cgctgcggta ttatctgcag attcgtccgc tgtatctgaa aaaccgcctg
     1981 cctcgtcaac cgctttgcgc tcggcttcat tcatatcggc aagctgttct tgggctgtaa
     2041 aagtaaaatc ttcggtaggt gaaggttctt ctttgctttc ttcgccagcc atacgcgctt
     2101 ctttttgctt ttgctcatat tggctgactt cttcttctgt atagatattc aggcagtatt
     2161 cgcaatgaaa tttttggtct ttgggatcaa aagtcaacgg accgccgcaa ttaggacatt
     2221 tatgtgtaag aacatccatc agctgcacac ctttctaaat aaattaatta acggggattc
     2281 ctttaaacgg tttaccacag tttggacaga atttagggat gccgttggaa agatccacga
     2341 tctcattgca ttcactgcat cgcatttgta gcttaggctg aacgttttct acaggtttgg
     2401 cagctccgca gttggaacag aattttccgg aattttctgt cccgcaaact ggacatgtcc
     2461 aagtatccgc atttccttgt gcagtttgtt gattttgttt ttctgcttgt tgtttgattt
     2521 gttcttggtt attttgagct gcttgagaaa gataagaacc atttgcattc attcccatac
     2581 ctacacccat aaagccggtc attgctcctg cttcattttt tccggctgct tccataccac
     2641 gtgcaatgga accttgcacg tatccttcac gtacgctagg atcacctagc atcgcccctt
     2701 cgttacgcat attgatcagt ttcacagaat catcggtgta agaaatgctg gctacagcca
     2761 cagaaacaat ctccatccca cgtaattcac gccaagaatc atccaatgca gtatccatgt
     2821 atttgcttaa ttccaaactc ttagatggta cataagaaat ccgttgacca tctgcagaca
     2881 tctgattgat tgctgattgt agcgctgtta ggaattcagc taaatactgc tcattgatat
     2941 cattgatttc cacttgtgtt ttgtttttag ggattgcgtt tgtataaaat aaaatcgggt
     3001 caacgacatg aatagaataa gtgccatgtg cccgtaagaa taattctgcg ttatagaaat
     3061 tgtcaaagta gttcaacggc gcgcttgtac cgaatttgat tccctttatt tcttggagat
     3121 tgatataaaa aacttgttgt ttctgcggtg tgacgccgcc aaatttgaat cggtcaaaag
     3181 tttcagcaat tgcttctttc agtgttccat tgaacatgga aggagctgcg tcatttttaa
     3241 tggtgtaata accttcctca gcggtataat cgatgatttt tccaccatcg accaaaagca
     3301 tcatcatgtt tggatagaca tggacgactg ttccgtctgt taaaacatct tctgttcctt
     3361 tacgattgga gccacgtttg tcatctttgc ggactttgac accttttgtc atgaccgttg
     3421 tatcactcat attgtctggt tcgatgactt ctagccattg atctgccaga ccgccgccaa
     3481 ccatacttgt tgctgctttg ataagaccca taaaaaattc ctcctctgat tcagctagtg
     3541 ccaaacaatt attgtttatt tattatacca taggaagatg tgaaacttct cggactagag
     3601 acggaattga aatggaaaat tgcgtattgt ttaaatacct gaaaaagccg agaaggatag
     3661 tagttttttt acctaatatc acgtataata agagatagtg aattttttaa aaaaggggga
     3721 tccttatggc atatcaagca ctttatcgag tctggcgttc gcagcgtttc gaagacatcg
     3781 ttggacaaaa agctgtgaca cagacactaa aaaatgctat tgtttcccac aaaacctcac
     3841 atgcctactt gtttacagga cccagaggaa ccggaaagac gagtgcagca aagattttcg
     3901 caaaagcaat caactgtccg aacagtaaag acggtgagcc atgcaacgaa tgcgaaatgt
     3961 gtcgatcaat tactgctggt acgcaagaag atgtgatcga aatcgatgct gccagcaata
     4021 acggagtcga agaaatccga tttatccgtg atcgggcaaa ttatgcgcct acaaaggcag
     4081 cgtacaaagt gtacattatt gatgaggtac atatgctttc aacaggagct ttcaatgcct
     4141 tgttgaaaac attggaagag ccaaaagaaa gtgtcatatt catcttagca acgaccgaac
     4201 ctcacaaaat accagctacg atcatttcac ggactcagcg atttgatttt aaacggatca
     4261 atacggcaga tattgtcgaa catcttgaat ttatcttgaa agaatcgact atcagctatg
     4321 aaccagctgc attgaatgtg attgcccgtt ctgcagaagg cgggatgcgg gatgcattga
     4381 gtattcttga tcaggcaatc tcgtttagtg aaggaacaat tactttagat gatgccatgc
     4441 aagtgacagg tagcttaact gacgaaatga tggacagtta cttgagtgct tgcgtgaaaa
     4501 aagaagtacc agaagcattg gaaacattga accaaattct cgcagccggc aaagaatcac
     4561 gtcgctttct agaggatatg ttacaatact gccgtgactt gctgatgtat cagcaagcac
     4621 ctaacctagt tgaggaacaa tcgagtacga tcagaagtac atttacagaa ttggcacaaa
     4681 ctgtttcagc ggatcagctg ttttcaatga taaagatttt gagcgatact caaaatgaaa
     4741 ttcgttttac caatagtgcc gctatttatt tggaagtcgc tacagtcaaa cttgcacagc
     4801 taccgtctgc ttctgttcct tcagcagatg tagcatttgc agatgatcag atagtaaaac
     4861 agctacaaca agagattaag gaactaagaa acgaaatcaa ggaactacgc agtaatacag
     4921 gaattgagaa tacaaaagta cagccggtac agcaaacaaa tcataaaaaa cagacaagta
     4981 cctatcgtgt accgacggaa cgagtatata aagttctgcg ggaagcgaca agacagcatc
     5041 tgaaccaagt aaaagaagta tgggaagact tgctgatgag tctatctgtc actcagcgag
     5101 caatgcttaa agcaagcgaa ccagttgcag ctggacctac aggactagtg attgcgtttg
     5161 attatgagat tgtttgtcaa agagcagcaa atgacgaaga attgcagctt tcggtacaca
     5221 acaacttaag ccgaatgatc aaagattatg cgcctgatcc tgtttatatt acaagagaaa
     5281 gctggccaga acttcgaaga aattatttga cgcaggcaca agaagaggga acagaaaact
     5341 cttttgcttc tgaagactca gaaggagaaa tcgagttgct tcctcaagaa caagaagtac
     5401 cagaagaaac acttgtagta aaagcagagg aactgtttgg ctccgaagct gttacagtgg
     5461 tagatgatta ataaaaagga gtagataatt atgatgcgtg gaatgggcaa tatgcaaggc
     5521 atgatgaaac aagtacaaaa aatgcaaaaa gaaatggcag aagcacaaga gctgctaaat
     5581 aaaacagagt ttacaggtaa agccaccaat gaattggtca aagcggtttt tacaggcgat
     5641 cgtcgcatga aagatatcca aatcaatgaa gaagtcgttg atcctgatga tatcgagatg
     5701 cttcaggatc ttgtcgtaat ggcagtcaat gatgcgatcg cacaaattga tgtggaaact
     5761 gagaaaaaat tagggaaata cgcaaaaggt ttgccaggat tttagtttta aactgaagga
     5821 gtaatgaata tgcaatatcc agaaccaatt gcgaaactga tcgacagcta catgaaactt
     5881 ccaggaatcg gtcagaaaac agcgacaaga ctcgcctttt atacgatcga tatgaaagat
     5941 gaagtagtaa atgaatttgc taaatctttg ttaagtgtaa aacgcgattt gcatttttgt
     6001 agtatctgcg gaaacatcac agaagaagat ccctgcgaaa tatgtagaaa tccttcacgt
     6061 gatcgaagcg ttatcctagt ggtcgaagag ccgaaagatg taatggcaat ggaaaaaatg
     6121 cgagaatatc acggacttta tcatgttctc catggtgtgt tatcaccgat ggaagggaca
     6181 ggtccagaag acatcaatat ttcttctctg ttgcagcggc tgcatgatgc agatgtaaaa
     6241 gaagtgatta tcgcaacaaa tgcaacaaca gagggagaag caacagctat gtatctctca
     6301 cgtctgatca aacccgcagg tatcaaagtg actcgtctag cacatggtct gtctgtaggc
     6361 tctgatatcg agtatgcaga tgaagttact ttattgaaag ctgtcgaagg aagacgtgag
     6421 ttataaattt ctaattaagt gttttttcag atacaatata aaagcagcac aagagatggg
     6481 ggcaacaatg tgaacggact atttattaca atcgaggggc ctgatggtgc aggaaaaacc
     6541 agcatcttga atgaacttta ccctcttttg aaagaagtag caaaaacaga aatcattcaa
     6601 acaagagaac caggaggcat tccgattgct gaagagatcc gagcagtgat ccttgatcca
     6661 aaaaatgatc ggatggatga acgtacggaa gcactgcttt atgcagcagc aagaagacaa
     6721 catctagtcg aaaaggttct gcctgctctg gcaaaaggga aaatcgtttt atgcgataga
     6781 tttgtcgaca gttctcttgc ttatcaaggt gcaggccgac gcatcggcgt taccgagatt
     6841 gctcggttga atgagtttgc tacagaaggt actacgccgg attttaccct ttatcttgac
     6901 gtagattcag acaccggatt aagaagaatc aagaaaaaca gacaaaatca aatcgaccgt
     6961 ttagattcgg aaggtttgga attccaccag cgtgtacgtc atgcttactt aaagctggca
     7021 gaagagaatc cggaaagaat ccacaaagtt gatgcaagaa aaagctttga agaagtgctg
     7081 caaacaagtt atcatacaat tattgaacag tatccgcaat tttttgaaaa ctaggaaggt
     7141 gaacgtttat gaaaattatt ttagcgatta tccaagacaa agacagcaac cgtttatcaa
     7201 atgaattgat tgatgcaaat atccgtgcca caaaactatc atcgacaggc ggcttcctta
     7261 aggctggaaa cagtacgttt atcgtgggta ttgaagatga ccgtgtagaa gaagcattgg
     7321 aaatcatcaa aaaaacgtgt gaatcaagaa agcaatttgt atctacacct gttacactgg
     7381 atatttcgat ggatgggggc gtcccttatc cagtagaagt agaagtaggc ggtgccacag
     7441 tcttcgtctt acctgttgaa ggattccatc aattttagga ggagctgagc tatgaatcaa
     7501 gaaataaact tggctcaaat gcaaccgatc gtgtatcagc agcttcaacg gagttttgag
     7561 catgggcgta tcgctcatgc ttatcttttt gaaggggaga aaggaacagg aaaacatgaa
     7621 gtaggtatat ggcttgcaca gcacctgttc tgtacccaaa tgaaagacaa tctgccttgt
     7681 ggaaaatgca acaattgtca gcggatcgaa aagaaagaac atccagatgt tttggttatt
     7741 gaaccggaag gacaaaccat caaagtcgat cagatcagac gtttgcagac ggaattcagt
     7801 cgcagcggtt acgaatcaag aaaaaaagtt tttttgatca aagaagctga aaaaatgaat
     7861 agcagtgcgg caaacagttt gttgaaattc ttggaagagc cccctggaga ttttttagcg
     7921 gtgttagaaa cagatgcagt aggaagaatt ctccctacta ttcaatctcg ttgtcaagtt
     7981 ctgcattttc aagaactttc gaaagaagca ctgatccatc gattgcaaga agagcagatt
     8041 ccattggaaa aagcaaaact tttggctttc ttaacaaaca gtttaggaaa agcagttgaa
     8101 atctctcaag atgaatggtt taatgatgct aaggacttga tttatcaatg gttcgtttac
     8161 ttgcaaaagc aagatactca agcctttatc tatgtccaaa aaaaattggt aaaatcattt
     8221 aaagaaaaaa cacaacagtt tacaggatta tctgtcttga tgttttatta tcaggaagca
     8281 cagcgtcaag cagtagcaaa cggcgaatat ggcaaagtga aacagataaa ccagataatt
     8341 gaacggatat tgatcgcaga gcagaaacta cgcagcaatg tcagttttca ggctgttgcg
     8401 gaacagttcg ttttgcatac gattttttag cttgccgcaa aggatgatga tcaaggagga
     8461 tggaaatggt agaagtagta ggtgtccgat atagagatgc aggtcatatc tattattttg
     8521 caccaggaga ctctgaatat gtttatagtg acaaggtatt agtcgaatct cagcaatcct
     8581 tacatattgc tactgttgtc cttcctaaaa aagagattga tcccaatgac cttcctgaag
     8641 ctatccgtcc gatcttgcat aaagcgtcac aagctgaatt agaaaaagac aaaaaaaaca
     8701 aaaaagatgc ttatgatgca ttgaaaatcg caaaagaaaa agtgcagttg cgtgagttgg
     8761 aaatgaaaat ggtcaaagcc gaatacacat ttgatcgaaa taaattgatt ttttctttca
     8821 cagcggacgg gcgaattgac tttagagaat tggtaaaaga tctggccgct gtatttcgta
     8881 ctcgtattga gcttcgacaa attggtgtcc gcgatgaagc aaagataata ggcggaattg
     8941 gtccatgtgg gcgtccatta tgctgttctt cttttttagg cgatttcgtg cctgtatcaa
     9001 tcaaaatggc aaaagatcaa agtttatctt tgaatccaac aaaaatttcc ggtttatgcg
     9061 gacgcttgat gtgctgtctg caatatgaga gcgaagaata cgagacagcg aaaaaagaac
     9121 tccctgactt tggaaaagta attgctacac ctgatggtaa agggaaagtc gtagggttga
     9181 atctgcttag ccgtatcgtt agtattcggc tgactggacg ggaaaatcct gtcgaatatg
     9241 aatgggatga attgaaagaa tttatccaag taggtgagat gaatggataa gagatcatta
     9301 tacgacggat tgaatcaatt agaatcagag ttgagaaata cattaacaga acttgttgaa
     9361 atgaagcatg ctttgcatga gatcgtagaa aaaaatacaa ctttggagat ggaaaatcaa
     9421 cgtctgcggg aacacctgcg ggaattgaac caagttgcac aagctccagc tgaaaatgtg
     9481 aagcaggaac tttctaagtc tcgtatgaat cttgaaaaaa tttatgaaga aggattccat
     9541 gtctgctacg atttatatgg atcaagaagg gacaatgacg agccttgtgc gttttgtcta
     9601 gaagtgatct atcgcgaaag cggtcgatga ccaataacaa gaggagactg gagcggagaa
     9661 atactgctcc agtcttttta ttaaggagat tgtgacagat gtataatcaa aaaagtttca
     9721 agggaaatcc ggtatatggg gatttatatc ttgtaccgac gccaattgga aatttagaag
     9781 atatgagcta cagaagtgtt cgtatgctgc aagaatccga tcttatcgca agtgaggata
     9841 cgcgtaatac acaaaagtta ttgaatcatt ttgaaatcca tacaccccaa aaaagtttac
     9901 atgaacataa ttataaagaa cgtgtccctc aattgattgc tttgttaaaa gaaggaaaaa
     9961 cgattgctca agtcagtgac gcaggaatgc cttccatcag cgatcctgga catgagctag
    10021 ttttagcctg cataaaagaa gggatttctg tgatctctat cccaggacct acagctggga
    10081 tgacggcttt gattgcttca gggctagtgc ctcaaccatt tttattttat ggcttccttc
    10141 cacgtaagaa aaaagaacag aaagaaacat tgaaaaagct aaaagaacag acagttacgc
    10201 tgatttttta tgagtcacct tatcggatca ctgctacatt gacgaatttc ttagaaattt
    10261 ttggaaatcg ccaagtcgtt ttgtgtcggg aattgacaaa ggtccatgaa gaatacctta
    10321 gaggaagtac ggaagagcta ctcgcttata tcgaagaaca tccagtaaag ggtgaatgtt
    10381 gtctgcttgt agaagggaat ttgttcgaaa tgccagtgac ggaagttgat gaagagttgg
    10441 gaacgttaaa ggaacaagta gaagcaaaga tcgcttcagg cgaaaaacca aatgctgcta
    10501 tcaaagcagt agcgctgaaa aatgggctga aaaaacaaga agtctaccgt cagtaccatg
    10561 aattagactg agatagtata agttgtctat aatgaaagac taatgaggaa atcagctact
    10621 aacttttcaa atctcttgaa cgcgaacgaa tgttcttgta taatgaataa gaggtgagga
    10681 tgatggagct gcaatttcct ttacataatg atacatcaag aaaaatcatt catattgata
    10741 tggatgcttt tttcgcttcg gttgaagaac gagatcatcc tgagtttcgc ggacatccat
    10801 tagtaattgc cagacacccc agtgacactg gtgggcgtgg agtagtgact acagcaaatt
    10861 atgaagcaag aaaattcgga attcattcag caatgagtgc acaaaaagcg tatgagcttt
    10921 gtccgcaagc catctttaag cctggcgacc atcataaata cagcgatatt tccaaacaag
    10981 tacgggaagt attctatcgt tatacagata ttgtggaacc agttagtatc gatgaagcgt
    11041 atcttgatgt gaccgaaaat aaaatcaatt gcaagtcagc catcaaaatc gcccgaatga
    11101 tccaagcaga tatttgggag agtgtccagc taacatgttc tgcgggagta agttacaata
    11161 agtttttggc caaattagca tcagactatc aaaaacctag aggaatcacg gtagttgccc
    11221 ctcaagacgc tgtgccattt ttaaaagctt tgccaattga aaaatttcat ggggtaggga
    11281 agaaaactgt acctagaatg catgagctag ggatttatac aggaaaagac ctatatgaat
    11341 gcacagaaat gatgttgatt cgaaattttg gaaaaatggg ctattctctt tatcgtaaag
    11401 tcagaggaat ccatgattct ccagtaaacg ttacaagaga gcgaaaatct gtgggaaaag
    11461 agcatactta tgggcagccg ttgcaaacgg aagaagcagt gctgactcag ttgcgccaac
    11521 tagctgaaaa agtagaagaa gcactgagaa gagtccaaaa acatgggaaa acagtagttt
    11581 tgaaggtcag gtacacggac tattctactg tgaccaaaag agtgacattg ccagaatata
    11641 tctataaaaa agaagcactc ttttatcaag caagccttat atgggaggaa atcttgggag
    11701 tagaaaaagg aatccgattg ttagggatta ctttgacgaa tctggacccg atgacttatg
    11761 aaaacatcgt ccttccatta tgggaaaatc aagaaatata aaaacagctt tgagagttga
    11821 aaccggaaat ccgttttctc tcccaaagct gtttttatat caagtcttgc tttttgattt
    11881 gttcataggt ttctgttgca tccgtacatt tttcccaaat cacaacttga ttggattcta
    11941 aagatttttg gacgtcaatg atccgctggt tgctacttcc gcgaaattgt agttttaggt
    12001 cacgtttgct taattcaaag cgtccgtcaa caaggatatc gatttgcgaa aggagttcta
    12061 atttatcttc tgaatcttgt agcaattcat caaaggtata gcctgtccat gaccagatat
    12121 cttttgtcgt accgaactct tttcggatac gtcgtacgac ttgcagacaa acatctgtat
    12181 tcaaaaaagg ttcgccacca agtaaagtta atccttgcac gtaatcgttg cgcaagtcag
    12241 cgatgatttg ttcttctaat tcaggtgtga atggtttgcc atatcggaaa ttttgtgcag
    12301 ctttgttgaa gcagccttcg caggcaaata agcagccact aacgtaaaga ctgttgcgga
    12361 caccttcacc atcgacaaaa ttaaacggtt tgtagtcagc aatatagttt tggctgtagt
    12421 cttttgctaa ccattctttt ggttttggat ttctcataaa aaaacttctt tcatttaagg
    12481 ttcaaagata tagctattga agataaaatc tactcgacca cataaataag aacaaactga
    12541 cctaaatcca ttgttcaaga aagcttgttc ttattttgat agagggcgtt caaagataca
    12601 aagccatcga aagagggtgg tgaaaaaaga cttgtcatag cctcttattc tgaataaacg
    12661 gtgttcaaaa gcttgcatct gcagtaataa gcccaactaa acgaaaaata tgaggaacta
    12721 tttttgttta gctattctta ttacttgatg caggacagct ttttcacaac ctcatttcat
    12781 gtgtttcaca cgtgaggaaa tttctttatg gcggccatgt accatcggac gagcttgcgg
    12841 atttcctaaa tagccacaag tacgtttgac tacatcacat gtttttggat catgatttcc
    12901 acattctgga cattcgaacc ctcgttctgt cggtttgaaa tcaccttcga aaccacattg
    12961 atagcaatga tcgattggcg tattcgtacc aagatacccg attttgtcat atgcaaaatc
    13021 ccatacagct tctaaagctt tcggattttg ttggagcata gggtattcac agtagtggat
    13081 gaatccacct gaacaatatt ttggataatc cttctcaaat tccaattttt caaatggcgt
    13141 tgggttttta cgcacatcat agtggaaact gtttgtataa tattctttat ctgtgatatt
    13201 ttcgatgatg ccgaattttt ctgtatccaa acggcagaaa cggtcagtta gactttcact
    13261 tggagttgaa tagacactga aatgataacc atattcatcc ccccatgcat ctgcattagc
    13321 ttttaattct ttgacgatat caagtgtgaa agcttttgct tctgggtttg tttcccattc
    13381 gccaccgaag aaagctgaag caacttcata caatccaatg tagcctaacg atacagtagc
    13441 tcgacggttt ttgaataatt cattgacaga atcggtacgt gaaagacgtt tcccgaaagc
    13501 accatacata tataaaattg gcgcatttgc tggaatggct tctttacaac gttctacacg
    13561 atagagaagc gcatctttca tgatttctaa gcggtcagac agtaactgcc agaatttatt
    13621 ctgatctcct tttgattcga tcgcgattct tggcaaattc aatgtaacaa cacctaaatt
    13681 cattcgtccg acatttactt cttgaccatt ttcgtctttc cacccttgca agaatgaacg
    13741 acagcccata ggtactttga aacttccagt taattcaacg attttatcgt aattcagaat
    13801 atcaggatac attcttttcg ttgcacattc caatgctagt tgtttgatgt cgtaattagg
    13861 atcttcaggg tttaagttca cacctttttt caaagtgaag atcagtttag ggaagatagc
    13921 tgtccgcttt tcactcccta atccattgat tcgaatctgt aaaattgctt tttgaatttc
    13981 tctttcaaac cagttttctc ctagtccaaa tcctaatgaa gtgaaaggag tttgtccatt
    14041 tgaagtaaat aaggtattga tttcatattc caaactttgc atagcatcga agatatcttt
    14101 tttggttttt gcttttgcat attctttttg gcgttctggc gaatcgatcc attcttccgc
    14161 gtcttttaga tgttttttat agttcaattc tgcaaaagga gcaagcaatt catctgttcg
    14221 gtcggcagaa cagccaccat attgacttga cgctacattg gcaataatct gtgagatttg
    14281 tgctgtggcg gtctggatag attttggcga ttcgacttcg gcattgccga ttttgaaccc
    14341 attgttcagc atgcctttga aatcaatcaa gcagcaattc gtcatcggtg tataaggatg
    14401 ataatccaaa tcatggtaat ggatatcgcc tttttgatgc gcatttgcta tatgtggtgg
    14461 caacattttc agtccgattg attttccaac gatcccggct gtcagatctc gctgtgtatt
    14521 gaaaacatta ctgtctttat ttgcgttttc gttcacgaca gtttggtcct tgttgattag
    14581 cttttcaatc gaaaagttga tatctgttgc tttactgcga gcaaaatctc gtttggtacg
    14641 ataattgatg tactcttctg ctaattcata ttcgtttttt tctagtaata tatgttccac
    14701 gatgttttga atctcataga ttttgacatt ttcagcaaac cggcttttga tttcttggac
    14761 aaccaagtct gcaatcattt gaattttttc ataagtaagt ggtgataatg agccgtgaat
    14821 tttcttttct gctttgatca gtgcttcatt gatcttttgt tcatcgaaat caacatggcg
    14881 gccatcgcgt ttgatgactt tcatctggga tgtctctggc tgctgagtaa cgacagtttt
    14941 ttcttcataa tttactttca ttatcttcaa ctcctgctaa attgttcttg tctatcataa
    15001 aacaaaatcg gggaaggagc aactaaatat cggctatata tagtattcaa tgtgctgtat
    15061 aattggattt ctatactata tgtagtgtat attttattgt gaaaaaaggt gaaaacttgt
    15121 agctggtgga tttgtttttt gcatatttaa ttttgctgaa taaaataatt gaataaaatt
    15181 ttttattcag tccaagtaaa agtgacaaaa acataagagc aaattattcg tagatatgct
    15241 actatgcaaa taaatagaaa tgaaaaatga aagaggagga gaagaattgg aaaagatgat
    15301 cgaagtgaaa catctaagta aaagctacgg cagcaaactt tctcccgtca aagccttaga
    15361 taatatctca ttcacagtag aagaaggcga gtttgttggg attatgggcc caagtggtgc
    15421 aggaaagaca acgctgatga atatcttgtc cacgatcaat ttgcctacga tgggcagtgt
    15481 ctcgatccaa ggaaaagaaa tcacaaaaat gaaaaacagt gagctcagtg actttcgtcg
    15541 aaaaaaacta ggattcattt ttcaagagtt caatttgatc gataccctaa atgcaaaaga
    15601 caatatccta ttgcccctag cagttgaacg gatgactaaa gaggaaatgg aaaagagagt
    15661 aagacatgta gctcaattgt tgaatataga agaactgctc aaacggtatc ccgatgaact
    15721 ttctgtcgga cagagacaac gaattgcagc tgctagagca cttgttgttc agccacaagt
    15781 gatttttgca gatgaaccaa ctggctcttt ggactcgaag tcggctacag aattgttgaa
    15841 ctacttgaag accatgaatc aaaaagaaaa agcaacgata ctgttagtca cccatgatcc
    15901 atatacagca agctactgcg atagaatttt atttattaaa gatggtgtga ttttttctga
    15961 agttgtcaga agagggacaa gaagagagtt ctttgagaaa gtaatagaca tgcaggcgac
    16021 gattggcgga ggaggaaaaa tgaatgctgt ataaactagc cgttaaaagt ttttttcggc
    16081 agtcacgagg ctatcttgtt tattttttca gtttgacttt atccgtaatg atctattatt
    16141 ccttcagcgc aatgacctac gatcaatctt tagtcagaag agcaagtcaa gatacttcga
    16201 tagatggtgg attgagtttt ggtggattta tcattactgt tgttttgctt tttttcgtat
    16261 tcagtgccaa ccgatttttc ttgaatagaa ggcaaaagga aataggcatc tatcagcttt
    16321 ttggtatgaa taaactgcag atatcaggta tatatgtgtt agaaatcatg ataattggat
    16381 tgttcgcctg tatttctggg atattactcg gtattatttt ttccaaactc ttttcaatga
    16441 ttttggttcg aatgatggat atggatctta ctagtccatt ttttatctcc attccatcag
    16501 tagtagatac tttatttgct tttttcatca tccttttagc agtatctgta tattcaatat
    16561 ggaaaatatg gcgctatcca atgattcgaa gttttggaga aaaagaacaa gcagatagtt
    16621 caacaatgcg cttcaggaca aggcaccgat tactcgcagt catcgggtta ctacttttaa
    16681 cgagcggata ttttggagct agtcatttta gggaaatcac ttcatggatg atcagttatc
    16741 attatcttga tacattagtt ttcttttttc cttttttaat ttttttcgtt tgtgtggtcg
    16801 gtacttattt gtttttctgt tatacatttc ggttattttt gatcatattg agtaaatgga
    16861 agatttatta ctatggtatc aattttttga taatgggtaa cacgcaagtt catttgctca
    16921 aaagttggcg tacaaattct ctgattacag tcattctcgg aatttcttta atgacaattg
    16981 gcggcacggc tagtatctct actattttat cctaccagga gaaagtttcc tccccgatgg
    17041 actatcagtt tgatgttgag acagagaaaa aaatcaagcc gattctagca gaggaagggc
    17101 agaagataac taaagaaatc accttgcatt ataaagtcat tgggagtatc tatagtcaaa
    17161 atctcgaaga acctatgttt caaactgcta atctgatcac ggaaacacaa tatcaagcct
    17221 tcagaaaagt taatccagat ttgccgaaca tttctttgaa atcacctggt catactgttt
    17281 tgcttgatca aatgcaaact ctttttcgga atatttcatt atatggatct agtattggac
    17341 tgccagataa tcaagaattg aagattcaac aaatgcttcc aagtgtatta ggtgacgaaa
    17401 caatggtata tctaagtcct gtactgattg tagatgatac cgtttttgaa aaagcaaaag
    17461 gtgtggatta tcaagtagtc aatgtagatg tatcaggtgg aaataatgaa aaaatagatg
    17521 aacgactgag caatgacctg tcgataaagt ggctcaatgc tgtttattat tcttatgata
    17581 tcgtaaatgg aagtctacaa ggagaaatta gcacaaagaa acttccggat gaccaaatga
    17641 aggaagcaca atttagccaa gaaagctctc gtttgaattt ctcttccagc tattcgaaat
    17701 tgcgtgcagt taatcggcga atcggtatca atatttttgt cacactattt gtcgggatga
    17761 tcgtgattat tgctactggt agtattttga cagtccggca attagcagaa gcagaggaag
    17821 aaaaagaaaa ttatcaccta ttgactaagc tggggattcc acaaagaagg atcagacgga
    17881 tgatcttcga gcaaaacgca cttaccttct ttccacccat gatattaggg atcactcacg
    17941 cagtatttgc aatcaatgtc ttcactcagt atataaaaac tgccgattac tggttggctt
    18001 atcttgtgag tggcttattg atccttatct atcttttgct ttatgtcatt acgagtcagt
    18061 tatattgtcg gattatcgag gaatgatata cggaataaga caagataata aatagaacta
    18121 aattttgaat accatttatt tggaataaga ggttgtgaca cgatttttgt cacaacctct
    18181 ttatttttta tattttttaa aagtacgaga aattataaag aaaaaagcag tgatttgatg
    18241 tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa aaaaacggtt tctgtaagct
    18301 agacacatgt ttctttttgt caaaattagt tacactttgt gtgaggttat atgaggaggg
    18361 aatccaatga acaaatggcg ttctaatcca aaattgaatt attggggaaa attcatcatt
    18421 cgaacaattt tttatagtgc agtactgctg ttcctgattt atctgtatca ctataaaaat
    18481 attcaaggag gaacttttat ctataacgaa ttttaggagg aagagtatgg aaatcaaaac
    18541 gattattgaa gcaatcgatg attggggaat aaatgaacct gatcgagttg cttatcaggc
    18601 ggatgagacg catacttttg gtgaattgaa acaagcatca gacgcacttg cttattattt
    18661 agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt ggcaatttgg agtttgaaat
    18721 gattgtctca tttctagggg ttgtcaaatc tggccatgcc tatttgccga ttgaagagca
    18781 tacacccaag gagcgtatcc tatcgatctt tcgggtggct aaaccatcta tggtcatcag
    18841 tatcggggac tggatagaag atattccttc ggttcctgtc ataacaaaaa aagaatttca
    18901 agaaatcaag aagataccag taggatttta tcccagaaat agcgtaaaag gtacagaaaa
    18961 tttttatatt atttttactt ctggtactac tggagaacca aaaggcgtac aaatcagtca
    19021 tgataatcta gtcagctttg ttcaatggac gatggaagac tttgggatca agcctggtat
    19081 gcatttctta gcccaagcgc cattttcttt tgatttatcg gtattttcta tttatcctgc
    19141 attagtttct ggcggaatgc tgaagccgtt aaataaagca gttgttcaag actttcgtca
    19201 attattcgcg acattgccag ccttgaagtt gaatgtgtgg gtttctactc catcatttat
    19261 ggatatttgc ttgatggaac ctacattcaa tggagaaaat gtgccggaat tggacatgtt
    19321 tttattttgc ggagaagaat tgacgaaaaa aactgcagaa agtctacttg atcgattccc
    19381 aaatgcacgg atatataata cttacggtcc aacagaagca actgtagcta tttcaagtat
    19441 tcagattgac cagcatgtac tagatagtta tgaccgacta ccgattggct acgtaaaaaa
    19501 agacacgaaa gtttctattg tccaagaagg taaacctgtt tcaaaaggag aaacgggtga
    19561 aatcattatt gcaggaccaa gcgtatcaaa aggttacctg aacaatcccg aaaagacagc
    19621 tgctgcgttc ttttgtcaaa atgacttagc aagttaccat acaggggatg caggtagact
    19681 agatgaatat gggcttttat tttatgaagg acgtatggat cagcaggtca aattacatgg
    19741 ataccggatt gaactcgggg atatcgaaca ttatctgctc caagacaacc gaatcaaaca
    19801 agctgtcgtc gtaccaaaat accaaggaac aaaagtacag cagctggttg cttttgtcgt
    19861 attagaaaac aaaacgaaac agccggattt tcagttgact aaatctatca aagaacaatt
    19921 actagaagtt gtaatggatt atatgattcc tcagaagttc cattatgtcg atctgctgcc
    19981 tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca gctgaggtga atcctgaatg
    20041 atgttacctc atatgatccc ttatgcagag ccgttttatt tttttctttt agccattgcg
    20101 cttgtaccga tcgttctatc tttattgata tgggaaaaaa gattaccagt ttatcaatca
    20161 gcagtgacac ttttcttttt atttgtcagc tttggaggta attattggca acagggagcg
    20221 gcgctgattg ggtatgtttt gtggcagacg atacttgtat ggggctattt tgtttatcgg
    20281 caaaggaaaa atcagacagg atggtttttt gccgccgtta ttttagcgat aattccactg
    20341 tttttaacga aagtgactcc tttttttgaa catggaaaag catcgttact tggtttttta
    20401 ggaatctctt atttaacctt taaatctgta caaatgatca tggaaataag agatggcatg
    20461 atcaaaacat ataagccatt tcgttatatc cagtttctgc tgttctttcc aacaatctct
    20521 tctggaccaa tcgatcgcta taggagattt gaaaaagatc agctccatcc gcctgttcgt
    20581 gataaatatg ttgatctttt gggaaaaggc gtccaaaata tttttgtcgg ttttttatat
    20641 aaattcatta tcggttatta ttttggtcaa gtactgcttc ctgtagtggg acgcattgcc
    20701 ttacagcatg gtggtatttc atgggcatta gtaggttata tgtacgtgta cagtatgtat
    20761 ttgttttttg attttgctgg ctatagctta tttgcagtcg gtacaagcta catgatggga
    20821 tatcagacac caatcaattt caataaacct tttttatcat ggaatatcaa agaattctgg
    20881 aatcgttggc atatgacgct gtccttttgg ttccgtgatt atgtatatat gcgattgatg
    20941 ttcttcatga tgaagaaaaa attttttaaa agcaagatcg taatgtcaaa tatcggttat
    21001 tttgctttgt ttcttctaat gggtatatgg catggattga cgtggtacta catcgtatat
    21061 ggactgtatc atgcttgtct gatctgtctg acagatgcat ggatccgatt taagaagaag
    21121 cataaagagc agttgccttc taacaaattt acgcatggct tagccgtatt tttgacattt
    21181 caagcagtat gttttagctt cctgattttt tcaggatttt tagataaact tttttttagc
    21241 taacgaatag tgacatatta atttatataa caaaggagaa aaggaaaatg gaagaacaaa
    21301 ttttatcaat cttagaagaa ataacaggaa cagatgaagt aagaaaaaat cgtgatattg
    21361 atctatttga tgaagggttg cttgattctt tagcatctgt tcaattatta gtcgagctag
    21421 acggagagct ggatatccaa gtccctgtat ctgaattcga gcgagaggat tggagcacac
    21481 cgaataaaat cattcaacaa gcaactgcat tgaagggttg agtatgaaaa agaaactttt
    21541 tgctattttc ggcccggttg ttttagctgt tggcttactg tttgtgtttt ttacctcctc
    21601 ctatcaagta gatatctcta atccagaaac gattagacgt gcctcttctt ccatgtcagt
    21661 gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta tctgaaaaac aatatattcc
    21721 tttttttggc tcaagtgaat taagcagaat cagtccattt catccgtctg ttctggcaga
    21781 aaaataccag cgaaactatc gtccattttt gctgggagca cccgggacac aatcgttaag
    21841 ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa aacaagaaag tcgtctttat
    21901 tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact gattacttta atacgtatta
    21961 ttctgaactt caaacatacg actggttgtt ttcaatgaag aaagtaacgc ctgctgatcg
    22021 ctatttagct agaagattat taactttctc aaaagtaaaa gagaatgata cgttgacggc
    22081 gattctgcaa acgatcaaaa aagggaagct acctttacct gaatctttga atcagctgcg
    22141 cagccaatgg aacatgctga agagagagga tgaagtattc agcaatattg ggttgaaaga
    22201 tcgacagcaa aaaatcgatc atgagtcaaa acgattacca aaacaatacc aagaaacaga
    22261 attatccatt ttagcaaatc aaattggaga acgagaaacg acgaataatc cattcgggtt
    22321 gaaaaacgat ttttatacgc atcgtatccg agcccacgag ccagagctca aacagtcaca
    22381 aaaaaattgg gattatcgtt tttcgccaga attttctgat tttcagctgg tcttggatca
    22441 attagcaaaa aaccataacg aagtattgtt catcatccca ccggttaacg agaaatggag
    22501 cgattatacc ggactatctc aagaaatgtt acaaggattt gccaaaaaga tcaaatttca
    22561 attaaatagc caaggattca atcgaatagc tgattttgtc aatcaagcgg gtaccaacta
    22621 ttttatggaa gacacgatcc atctaggctg gaaaggctgg ttggctgcag atcaacagat
    22681 cagaccgttt ttagaagaaa atcatattac tgctagtaag tatcatcttg acgatgcttt
    22741 ttttagcaaa tcttggcaac atcaaatacc agataaacta caattaaaat aaagaaaaca
    22801 ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt ctgaataaaa gataaaaata
    22861 ccaagtttgc ctgaacaaaa atcataagca atggtataat cacaatatga atatagaaat
    22921 gggggaaaac atttggatac aaaagaagaa cagctactta ttgatttaac ttctgcaaaa
    22981 ggagtacctg gaaatgaaga agaggtaaga gaagtattca gagaatatgc caaaccattc
    23041 gctgatgata ttttttatga cggtttaggc agtgtgattg ccaaacatgg tgctggcgga
    23101 ggccccaagg tctttatttc tggacatatg gacgaagttg gttttatggt aacaaaaata
    23161 accgaaaaag gattcttgga attccaaact cttggaggtt ggtgggggca agtcatgctt
    23221 gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt gattggttcc
    23281 aaaccaccac atgttttaac acctcaagta agaaataaac cttatgaaat aaaggatatg
    23341 ttcattgata ttggcgcatc aagccaagaa gaagccaaag agtggggcat acgtcctgga
    23401 gatatggtca ctccatatat tgaatacaaa cgaatgaatg gcagtaaata tttactggca
    23461 aaagcatggg ataatcgtat cgggacagct gtttctctgc gagtattaga gaatctatct
    23521 aaagaagcac acccaaatgt tttattcgca ggaagcgatg tgcaagaaga agtaggcttg
    23581 cgaggtgctc gaacaagtac acaccttgta aatcccgata ttgcttttgc tttagatact
    23641 ggtacagcag gagacactcc aggaatgaca ccaaaagaag cagattcgat tttaggaaaa
    23701 ggcccacaga ttttgatttt tgatgcatct atgatcccgc ataaaaaatt attgaatttt
    23761 gttattgatg tagcagagga aatggatatt ccattccaat atacagtgat ttctggcgga
    23821 ggaactgatg caggacaaat gcatttgaca agagatggtg ttccgtctgt agccattacc
    23881 gttcctgttc gttatctgca ttcccataca tcagtgattc atgaagatga ttatttgaat
    23941 acagtgaaac tagtcacaga attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt
    24001 cggagttatg aataatgaat aatagaatac ctatttctga atatcggaaa aaagcacgag
    24061 tttctttgga aaatcaatgg ggaatcaata cttggcttgt tttcttatct ataatattta
    24121 cgagcgtcat ccagaacttt tttgacggtt tcagtccttt tccggaagaa agcatgcagc
    24181 agcacttatt gagttttcta gtcgataatt tacttttatt cgcattcaac tatgcattgt
    24241 tttacattgc gctagtagtt gtacggggtg gcagaccaaa aagtaacctt ttattttcag
    24301 ttttccaaag aaagtatttt ggttcgatac ttggcatcaa tttgatcaat tcagtcatca
    24361 actggctact caacagttta atctttctgc ccatgttttt aatcagcgga gtgagtactt
    24421 atatgcagct gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca gaagattttc
    24481 ttgatatcag ctttgtttta tcgatgatag ggatgatttt tgtttcagca ctcatcatga
    24541 gtattgttgg cggtttattc caatttgctg catggacaaa gttcgactat cctgatttgt
    24601 cggttattca atgtttgaaa tatggctggt ttttattgaa agatagaatt ggtacgtata
    24661 ttttgttgca gctgtctttt atcggctggt atttgctagg agcaatcgct ttgttcatcg
    24721 gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc agaattttat gaacaagcac
    24781 ggattgaaaa aggaggtcca gacgcctatt ttacttagca tgtaagcagt ttattcatca
    24841 aaaaaataac ttttgtggta ggataaatct ttgaggggga atgtacgatg aaattaactg
    24901 ttttaggatg cttaggagcg tatccttata aaaatcaagg gacaacaggt tatttgcttc
    24961 aatctgacgg gttcaatttg ttgatcgatg caggtagtgc tacgctgatc aaattacaag
    25021 aacaccttga tccacttgat ctagatgctg tgatccttag ccattatcat catgatcata
    25081 ttgctgacct tggtgtatta cagtattact ggcaacttca tcctaagaga aatccggaga
    25141 cattgctttc aatttatgga cacacaaaag attccattca ctttgaagaa ttgacgttgg
    25201 aaaatgtctc aaaaggacat ccttattttg aagctgaaga attaaaaatt ggtccttttc
    25261 tgattacttt tatggaaaca atccatccag tagtatgtta cgccatgcgt atcgtagaag
    25321 aaacaacggg gaaagtattc gtatttacag gtgattcagg ttatttggaa agtttcgctg
    25381 attttgcgaa agatgctgat ttgtttctag cagataccta tctttttgaa ggaaatgaac
    25441 atcatcatgc ccatttcact tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa
    25501 agaaacttgt acttacacac ctacctcagt ttggtgattt agaacaattg aaaaatgaag
    25561 cgcaaaaggc agcaggcaaa gggattgata tagaacttgc tgcagttgac aaagtgttta
    25621 acatttagga ggaagaacat gatttttgta ccaaatgaaa accacgaccc aagaatcaat
    25681 ttagcaattg aacaatattt gctgcaagaa atgcctttag atgaacctat tttgctcttt
    25741 tatatcaatg aaccttcaat catcatcggc agaaaccaaa atacgcttga agaaatcaat
    25801 cgagattatg tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg aggcggagct
    25861 gtctaccatg attttggaaa cctgaacttt agttttatca tgcctgatga tggggattca
    25921 ttccgtgact ttgcgaaagt aacacagcca atcatccaag cacttcatga attgggtgtt
    25981 gctggggcgg aattgaaagg ccgaaatgat ctagtcattg atggcatgaa attttctggc
    26041 aacgcgatgt atgctaccaa cggtcggatg tttgctcatg gaacaattat gttcgactct
    26101 gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga
    26161 atcaaatcga tccgttctcg tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa
    26221 aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga aaattttcgg cgtagagtca
    26281 gtagaagaag taaaaacgta cgaattgact gatgaagatt ggaaaaaaat caatcgaatc
    26341 tctgaggaat attatcgaaa ttgggattgg aattacggga agtctccaga tttcaatttc
    26401 tctcgccaaa aacgtttttc tatcggttcg attgaagtcc atttgaatgt ctcagaagga
    26461 atgatcaaag aagcaaaaat ctttggcgat ttcttcggac taggagatat ctctgacgtg
    26521 gaaaaaaaat tggttggaca gaaatatgat aaagcatctc tatcaaaaat agtcagccaa
    26581 attgatgtga agaaatattt tggtgcgata gaaccagaag aattgcttac cttactttat
    26641 tgaaaataaa aaatagcgga atccacatat atggattccg ctatttttta tacaggattc
    26701 gggtaatttc gagagagttg aacagtgatc cgatcaatga aagtatcttt ttcctgcgtt
    26761 agtgttcctg tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa acgttcctga
    26821 tcatctaatt cttctgtgat gcctcgacta aataggtttg tatcgtaatg tgcactatgt
    26881 tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat ttttcgatac
    26941 agtggatttt cgatggcatg agtttctcta aaggaatgct ctaaagcgtg gatcaaaagt
    27001 tgagccagat cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg gaaggttatc
    27061 tgaatattcc gtttggcagc taacttctgg taacgcgcta agataagatt tgtcacctgg
    27121 tggttgcaaa aagaacacag catttcgaaa ttttttgctt cttctaatat cttttccaca
    27181 tagtttttag cttttgtttg gtccgtatgc attaaatctt gcagattctc catctcttgg
    27241 acaaattcca ggcgaagtgt atttacttct tggatatgct gtcgaagctg ctcataatgt
    27301 tgctccaaca aatcattttt gatttttaag aaatctactt gtctccgatc tttgtcggta
    27361 tttaagatgt gatcgattag attgtagctg ataataacga ttagaatgat aatatataaa
    27421 atcgtagacc aattgatcgt tgtttgatag atacctaatg ccgtttggat gtaaatcaag
    27481 caatggataa tggctaccaa gacgatccaa ggtgtacaag ctacataaaa acggttattc
    27541 ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag tgcttataac actagcaata
    27601 ttagcaatcg gcattaattc aggaagttct gcaggagtaa aagttgcata aagcagaacg
    27661 ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt accatttttt atagtaaaac
    27721 attcggctcc aatagtagca gatgatgaaa gctgacgtta agataataaa taaatgagat
    27781 aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta aaatatcttc tgatacagct
    27841 tcaaatccta atgccaaagc aaagcaactt aagagaataa gtgagtattc aagacgtttt
    27901 tgtttataca tcaagtagat cgtatagaaa aaggtcccta tagcgataaa aacagcgatt
    27961 atcatcgtca atacttgcgg cactgattgt gaataaataa aactaatcaa tggtccagtt
    28021 gttcctagat aaactttcgg tggcagtcca gcatagttct gataaggaga tgagactgta
    28081 agggtcaatt tttttccgat ataatttgct ggtaaatcta tcacagccaa tgagagtccg
    28141 ggattgcttt cttcctgata atctttccgt tggtataaaa tcttttcgcc tagatataca
    28201 gtaatccatt gatggtttcc tttgattagt aaatttgctt cgtccaacgg tttttcaagt
    28261 gtcgtttcca taatgaatgt ttcgtcttta tccacggtag gaatcgtacg agtgtacttg
    28321 ctgtcaaact taaaatcagg atcatctttt gtatagaaaa cccacgaatc ttttatttct
    28381 ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa agaaataagc aattcctgtt
    28441 aaaaataaca atgataatat aatgattatg ctccacttta tacgtaaata aaaagattct
    28501 tttttattat atatgttatt aaaaaaagac ataaaggtat tcctttctaa aacttgtaat
    28561 gttagggtaa acgtttttta aactaacgtc aattatttat aataaaaaat gagataaaga
    28621 tgaggaaata gaattagaaa catataatta ttaaagggaa acatttttac agctataatt
    28681 tgaaaaaaat ttatcgttat tatatgatta agaaggattg aataaagaaa gaagttgaaa
    28741 acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat tatattttta
    28801 attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc ttatattatt
    28861 gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt acgaccgaaa
    28921 aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc aggaaaaaaa
    28981 atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta tacaaaagct
    29041 gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat cggtagtccc
    29101 atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc atggattcag
    29161 gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa tacctttgta
    29221 gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta aaaacataaa
    29281 tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca ctcatgcagc
    29341 ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta tgcgcaatga
    29401 aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta cccagcaaaa
    29461 aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag aagtaccagt
    29521 tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc agcctgaacg
    29581 aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg
    29641 agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga aagctaaaaa
    29701 aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc aagcagcaga
    29761 tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg caaaatcaaa
    29821 atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg ttttgatggt
    29881 agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc cccgtggcaa
    29941 acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc atcccgattt
    30001 gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg aatcagaaac
    30061 aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat cgagggctca
    30121 attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata caggaaatct
    30181 aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat aaccttagaa
    30241 cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc aaaataattc
    30301 attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt atctataatt
    30361 attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc cgccttctaa
    30421 gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag aaactgaggt
    30481 ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag gcaaataaaa
    30541 taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac tcatcagaaa
    30601 agaggaagaa gatatgacga tactatctag aattttagtg actctagttg caattgaatt
    30661 tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa gtagggtttt
    30721 taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta agaaccaagg
    30781 agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt cttccgcatc
    30841 taaagaaatc acaagtatgc tattaatatc tatttttttt gttgcattat atggcagttt
    30901 gactagcgat cgttggattt tactcaaaca aggtgggcta gctctgttgg cattgctttc
    30961 tattttctta taaatataga aaaaataaga atttatctaa caggaaactt tgtgaaaaat
    31021 attactttat ctcatgaaat atgatattct aatgaatggt attttagaaa atttcatgaa
    31081 aaaggagtta gataggtgtt caaaaattat gtttcctcac taaaaaaaga attttcaggt
    31141 tatgatcttt caagatttca gaaagatctt ttggctggga tcacagtagc tgctgtagca
    31201 ttgcctttag ccttggcttt tggggtatca agcggagctg atgcggccgc aggtttgatt
    31261 acggctatca ttgccggtat cgttattggc ggtttatcag gcggttttta tcaaatatca
    31321 ggtccgacag gtgcgatggc agctatcttg atgtctattg cggcaacaaa gggaatgcaa
    31381 ggtgtattgc ttgctacttt tttagcgggt ctatttttac ttgcagcagg acttttccga
    31441 ttaggaacac tgacttcttt tatcccagca cccgtaatta cggggtttac ttcaggtatt
    31501 gctatcatta ttgcattagg tcagatcgat aatttatttg gtgttcactc ggaaggtaca
    31561 aacgttattg aaaaactaag tagttatcaa acgataggat ttccgataaa cacagcctct
    31621 ttgatcatgg gagggctagt cattttaggg atgtttatct atccaaaaaa atgggcgaaa
    31681 cgaatgccta gttcgctgct ggctatcatt ttggcaacag cagttatgct attgttccat
    31741 ttaccagtag caactgtggg taagattcca caaacattga tcagtagtaa tcgtttgaat
    31801 atgggagatt ttagcttttc tgccttgcaa caagtggcag ttccagctat tagcattgct
    31861 ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg ctggccgaat ggcaaataag
    31921 cccttagata gcaatcaaga attaatggcg caaggaattg gaaatctgtt gcttcctttc
    31981 tttggcggga ttcctgctac tgcggccatc gcccgtacaa gcgtggccat caaatctggt
    32041 gcacaaacta gattagctgg tatgatacat gcagttgtct tactgctctc catgcttgtt
    32101 tttgcgccag tgatgtcaca gattccaatg ccagcacttg caggtgtttt gattgtgacg
    32161 gcttggcgta tgaatgaatg ggaaacgatc aaggagctat ttactaataa atattggtca
    32221 gcagttctat tgtttatcct tacgatgatt tgtacagtta tctttgattt aagtattgct
    32281 atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg tcaaaagcgc tgctatcacg
    32341 atttcattag aaacgatcga ttggcaaaga atggggctgc cagaaacgga aaagctgaat
    32401 aactggacag tagtgtatat tagtggtcca ctgtttttta tgtcttcaga aaaactcaaa
    32461 aataaattgg aagaactaaa tcatcacgat ggtgtcattt tctccatgcg aggcgtacca
    32521 agtattgatt tgacggcacg tgcagttttt gatgaatttc aagaaaaagc agagcaaaga
    32581 ggacaaacga ttatctatac ttccttgcag ccagaagtag aaaaacaact ttctcacttg
    32641 tgggaacata cattcacgca tcaacatcaa acagttgctc atgcgttgac agctttacat
    32701 gcgaaatata ctgcaattag ccattaaaac aatgaaaaag aacagacaag actgtaaact
    32761 gaagatttta tggtcttgtc tgttcttttt aatattgctg taaaagtgaa gctagtactt
    32821 gccagtcttg atggataaca ggtacaaggg agcgattgta gaaaatagct gctggatgaa
    32881 aaagtggaag aattcgatag tttttttctg accattcgta ggcatccttt tgttctgtta
    32941 attgtaagac aggccctgta tagagttgac catgattttt ggtaaccgta tattctttgc
    33001 caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat taattttggc tggaccttca
    33061 tcagttcgta atccaatatt ggtgcatgcg caaatacttc ggaacgagta ggtgtccgat
    33121 ttggataaac agtttctgac tgttgtgtcc gtttattgat ccgatgagtc acgcgatacg
    33181 ggcggctcct tacagcactg gtgatataca cctcttctcg tgttaagcca gttgaagaaa
    33241 gtgcctgcat caattctttt ccagcttgac cgctaaaagg aacatgcgta tcaatctctg
    33301 ttttccctgg ggcttctccc acgagcatca gtttaggatg aagaggtcct tgcccagcga
    33361 taaatccttc tacaggacgg ccgttcattc ttaaagaagc ttcttgtatc agttcttttg
    33421 ggtattccat ttatgctcct cctttttctt ttattataag aataaaagaa acacaatgcg
    33481 actattttat ttggtagaac tgtgaggata gaaaagcaga agtattgctt gaatttttgt
    33541 tttacttttt gattttaaat ttagttgact aacggttgct gttctggtaa gctagtactg
    33601 ttgtaattgt ggactccaca gctacaaccg ctcagaacaa gtaataagca cagcatatgc
    33661 tgtcacttcg gatggcgagt ctaagtatat cagaaggagg tgctttatag catgtacgca
    33721 attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg gacaagcaat ctatgttgaa
    33781 aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg aagttatctt agtaggcgga
    33841 gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa ctgttgaagg tactgttgaa
    33901 aaacacggca aacaaaagaa agtcgttact ttcaaataca aacctaaaaa acattcacac
    33961 cgtaaacaag gtcatcgtca accatataca aaagtggtta tcgacgcgat caacgcttaa
    34021 tagcgtttga gaaagaaggc ctatatatga tcaaaggaac atttaagcga aatgacgcag
    34081 gtcagatcgt ttcattcact ttgaccgggc atgctgatgc tggtccttat ggaagtgata
    34141 tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt caacgggatt gcctcattag
    34201 ccggttttga gccgattgtt gaaatgaatg aagaagaagg cggttatctt tataccgaag
    34261 tcacttcagg aatgacacaa gaacaaaaca atattgctga gattctatta gaaaatcttt
    34321 tattaggatt acaatcgata gaagctgaaa actcagaata tatccaaatt aaaacaatta
    34381 atgataaata ggaggtgcag accatgttgt tatctatgaa tctacaatta tttgcccaca
    34441 aaaaaggtgg cggttctaca tctaacggcc gtgactcaga atcaaaacgt ctaggtgcta
    34501 aacgtgctga tggacaaaca gttacaggtg gatcaattct ttaccgtcaa cgcggtacaa
    34561 aaatctatcc aggtgtaaac gtgggtatcg gtggagacga cactttattt gcaaaagttg
    34621 atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa acaagtgtct gtttatccag
    34681 tagctcaaga agcttaattg aatcttatga ttaaagaggt tgtgacaaga cttttgtcac
    34741 agcctttttt gtttataaaa ataaaagttg tagtggaggc tacgaagttt actttgtctt
    34801 tttacactta atattttttt gccaatttgg aatcaggatg ttttgataac tatcgttttc
    34861 aattgatgga agctatattg tttgctatca gtgaaataat tatatggtaa acagtgttct
    34921 ttttcttgtt tccattataa aaatcgtatc gggatttttc gttcttttaa attgaacatt
    34981 tctatctatc cgcgtgttat caacaaaaca aaatcaggta aatagttgac gaaacagaaa
    35041 taaatacata gtattatgtt ttgaagccta agtacaatag tctatagaaa aggagcgata
    35101 ccattggcaa aacgcattga ccgaatttat atgtatgtca aagaaaaaac cgctcattta
    35161 acaaaggcag aatatgatca aggagtaaca acacaggaaa ttgctgaagt gttagggatt
    35221 cagcgaacaa atagcagtaa agatctgaat cagctagtgc gcgaaggaaa actgataaag
    35281 acagacggac gaccagtacg ttacatatat caaacactcg ttacgcacgt aaagccagtt
    35341 acaaaacatg tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa tacactccaa
    35401 atagatacta aggatatctt tgcaaaaatc attggagcta acggcagcat gaaaaattca
    35461 gtggaacagg caaaagctgc gattttgtat ccgccaagag gattaaattg tttgattaca
    35521 ggacctaccg gatcaggaaa gacttatttt gcacacgcga tgtatcattt tgcaaaagcg
    35581 catcatgtaa tagatgagga aacggaactg attgttttca attgtgctga ttatgccaat
    35641 aaccctgagc ttttgatgag tcatttattt gggtacgcaa aaggtgcttt tacaggcgca
    35701 gatgaagaaa aaatggggat tatcgatcaa gcggatggcg gtatgctgtt tttggatgag
    35761 attcaccggt tgccgcctga aggtcaagaa atgatctttt actttatgga tcatggaact
    35821 tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg
    35881 accacagaag acccaggttc ttctctatta gaaacttttg ttcggcgtat cccaatcaat
    35941 atcaaacttc cggcttttga aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc
    36001 atgattgccc acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga agatgtagca
    36061 aaagcattga tcggcagtgt gacctatgga aatataggac aattgaagtc aaatatccag
    36121 ttagtatgtg cacgaggatt tctaaatcat atgcaatcac ccgaaatctc gatcacaatc
    36181 gatgatttga cagaagggat tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca
    36241 atggcagagc tttctaaaat attggagcca aagataactg tagcaccaaa tgatacgatc
    36301 atgaaaatcc aatcggactc ctatgagtta ccttataatc tatatgatat catcggagat
    36361 aaagcagctt tactgaagtc ggatggtttg gatcaagaag caatcaatca tttcatttct
    36421 acagatatca acatccatct gaagtctttc tataaagacc atggtttttc ttttaatgca
    36481 gacagcaaat tagcagaatt tgtagatccg aaaatcatag aagtgaccaa tcagatttat
    36541 gtaatggtca aaagatcttt accatatgag tttcagcaaa actttattta tgctatgagc
    36601 ttgcatatca gttctttttt gaaaaaaatc aacttaggag aagaacggca tacgaatgat
    36661 aacatccgtg agatggcttt tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt
    36721 agttatatcg aaacgtattt tcaagtggag attccagaaa gtgaagacta ttacttgacc
    36781 gttctactcg tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca
    36841 gctcacggaa acagcacagc aagcagtatg gtccaagttg tccaacagtt gttagatgca
    36901 gagaacgtac gagcagtaga catgcctttg gatatggatc cgaaaacagc attggaccga
    36961 attgaacgca atgtacaaga agtagatgaa ggaagcgggg tcatactctt agtagatatg
    37021 gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc agtacgtact
    37081 gttgatatgg taacaacttc tctagttttg gaaactgtac gaaaggtctc agtattaggg
    37141 acagatctag atatgttgca tgaatcattg aaaaattttc gaggttacgc agaaatatct
    37201 tctgtagaat ctgttcagga agcaaaacct attcataaga aaaaagctgt tttggctatc
    37261 tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg agcggtaaac
    37321 aagcgtcaag aaacagaatt agaagttcta gctttgtcga ttgtggaagt gaaagaagaa
    37381 ctgccagtta tccaaaaaga atatcagatc atagctacta ctgggattac tgatttgaat
    37441 atccaagccc catttattcc tttagaacgt tttattgatc aaaatatcga agtcattttg
    37501 gatcagttgc tgatggaatc agaattggag gaaactgaat tcatctccct agatgaagag
    37561 tcagctaaaa atacgtgtgt tgagtttatc tctgataact tcatctttat taatggtagt
    37621 aagttaatcg atccaatgtg gcaattttct acacaaatct ctcagacgac gggaatcggc
    37681 gatgaagaat atggattcaa aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt
    37741 attattcgga atgaaccatt gactgtggaa gaaaacgaat tgacaaatac aacgaatgat
    37801 cctctttata gccaattggc agcctcagtt gttctgttgg aagatcagat aaaagtaaaa
    37861 gtgccgatag aagaaatgta ttatctgcta cgtctagtcc ataatcagtt agataaaaaa
    37921 gaatacactg tgccatgaat acacaaaaac gatacacttt tatagtgtat cgtttttgtg
    37981 tattggttaa gaatccttta aattcaatta gtgtatcgta aaaaagttgg cacgcttttt
    38041 gcttatagta gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg ggtttctgtg
    38101 aagtagtgag gaagaaggag ttttgcagat ggatattcga ttggcacgta tagatgatcg
    38161 tttaatccac gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg aacgtatcat
    38221 tgtgatcagc gatgtagtcg ctcaagataa attgagaaag tttctactaa aagaagctgc
    38281 accgccgggg atcacagcaa atgtaataac cgtgcagaaa atgattcaaa tctatcatag
    38341 tggattatta tcacgagcaa aagtgatgct tttattcacc aatccccaag atgttgaagc
    38401 ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc ggcggtatga gttttactga
    38461 aggcaaatca atgattacca attttgtttc agtaaatcaa caagatattg attcctttcg
    38521 ttacttgaac agtcaagaag tggagttaga gattcgaaaa gtacctgctg acaggaaaat
    38581 ccttttgatg gaactactag cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa
    38641 ggtcaaataa ttctgttgta atggttagca attattaata tttttgaatt tgttacaaca
    38701 aaagtaaata ggaggtataa atatggtagg gattatccta gcaagtcatg gacaatttgc
    38761 tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt
    38821 gattttgaaa ccaagtgaag gtccagatga cttgagagcc aaattggaag aagcggttgc
    38881 ttcttttgat aatcaagacg aagtgttatt cttagttgat ttatggggcg gaacaccgtt
    38941 caatcaatca aacacattat tcgaagagca caaagataaa tgggcaatcg tcagcggttt
    39001 gaatttacca atgttgattg aagcctacgc atcacgcttt tcaatggaat ctgctcatga
    39061 aattgcagca cacatcattg aaacagcaaa agacggtgta aaagtaaaac cggaagaatt
    39121 agaaccggca gaagcaccga aagcagcagt tgaagacgca caacctaaag gagcacttcc
    39181 agaaggaact gtggtcggag acggaaaaat caaatatgta ttagcacgtg ttgactcacg
    39241 tctgttacat ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa accgtattat
    39301 cgttgtttca gatgcagtat caaaggatga tctgcgtaag agactaatcg aacaagctgc
    39361 tcctccaggt gtaaaagcaa atgttattcc tattagcaaa atgatcgaag tagcaaaaga
    39421 ccctcgtttt ggaaatacca aagcattgct tttatttgaa aatcctgaag acgtattgac
    39481 agctgttgaa ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg cgcattcggt
    39541 cggtaaagtc gttgtaagta aagtattgtc tatgggacaa gaagacgttg aagcgtttga
    39601 gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg attcacgcga
    39661 taacatggat gatattttga aaaaagcaaa agctgaatta gcaaaagcgt aataaataat
    39721 attaaaattt ataggaggct tatcatgtct attatttcaa taattttagt cgtattagtt
    39781 gcctttctag ctggtatgga aggtattctt gatgaatttc aatttcatca accactagtg
    39841 gcatgtacat tgatcggttt agttaccggt aacctcgaag ctggtatcgt acttggcggt
    39901 acgcttcaga tgatcgcttt aggttgggca aatatcggtg ccgctgtagc accagatgct
    39961 gcattagcat cagtagcatc agcaattatt ttagttttag gtggacaagg tgttaaaggt
    40021 gttccttctg cgatcgcgat tgccgttcct ttggctgttg cagggttgtt cttaacaatg
    40081 atcgtacgta caatcgctgt tccaatcgtt catttgatgg atgcggctgc tgaagaagga
    40141 aacatccgta aggttgaaat gtggcacatc attgctgtat gtttacaagg ggttcgtatc
    40201 gcggtcccag cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc attccttgaa
    40261 tcaatgccag catggttaac agatggtatg gctatcggtg ggggaatggt cgtagctgta
    40321 ggttacgctt tggtaatcaa catgatggct acaaaagaag tatggccatt cttcgttatt
    40381 ggtttcgttg tagctgcaat ttctcaatta acattgattg ctctaggtgc tttaggtgtt
    40441 gcattagctc ttatttactt gaacctttct aaaatgggtg gttcatcaaa taatggaggc
    40501 ggaagcaact ctggtgaccc tttaggcgac attttgaatg actattaatc ttgaaggagg
    40561 aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt
    40621 ggcgctctac cttcattcaa ggttcatgga actacgaacg tatgcaaaac ggtggctggg
    40681 cattttctat gattccagca atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag
    40741 cattaaaacg tcacttggaa ttctttaata cacacccata cattgcttct ccaattttag
    40801 gggttacatt ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga
    40861 ttcaaggggt taaagtaggt atgatgggtc ctttagctgg tgttggtgac ccagtgttct
    40921 ggtttacagt acgtccaatg ctaggtgcac taggtgcttc attagctatg ggaggaaata
    40981 tccttggacc aatcatcttc ttcttaggtt ggaacattat tcgttgggca ttcatgtggt
    41041 atacacaaga atttggctac aaagctggtt ctaaaattac tgatgacctt tctggcggat
    41101 tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta gctgcccttg
    41161 tacaaagatg ggtatcaatc aaattcttac cagttgtatc aactgtaaaa ttagataaag
    41221 gtgcttacat cgaatgggat aaattgcctt caggtggtga aggaatcaag agtgcatttg
    41281 aacaagttaa taatggctta gccctttctc ctgaaaaggt aacaacatta caagacaact
    41341 tagaccaatt gattcctggt ttagctgcat taggattaac attgttatgt atgtggttgc
    41401 ttaagaaaaa ggtttctcca atcgttatca tccttggttt attcgtagtc ggtgttgttg
    41461 gacacgtcat cggactttta taatccataa aaatatagct cgggcgttgt cccgagcttt
    41521 ttttatatag tatactgttc ataaacaaat atctggaggg agaaaatggt tcaatcaatc
    41581 aatacaaaag tagacttggt aattgatgct acttcgttta cgggtctgac agattacggg
    41641 caaatcatga taggtgataa tggttttgag ttttaccatg cgcgagatag acgcaaatat
    41701 atccaaattc catgggaaga agtggattat gtactagcat cagtcatgct aaagggaaaa
    41761 tggattcctc gatatgccat tcaaacgaaa aaaaatggta catacacatt ttcttccaaa
    41821 gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag atgaagatag aatggtacaa
    41881 tcattgagtt tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa
    41941 aaaataaaga aaacttctaa gaggaggttt tctttatttt tttactttgc agaagaaaat
    42001 atgaaatagc aaaaccaatt atttgtcccc tttaaatccc atatgctaaa attgattagc
    42061 aaagaaggag gttatattat gagttggtta tggacattga ttgttggagc cgtgatcgga
    42121 gcaattgcag gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac gaatatcatt
    42181 gctggactag tcggttcaag tattggacaa gcgattttcg gcgaatgggg accaaaagct
    42241 gcaggtatgg cacttattcc atctatttta ggcgcagtga ttttggtagc agttgtctca
    42301 tttttctttg gaaaacgatc ataagttttt acaaaatagt aaactaaaag gagttgcagc
    42361 gagactgtga ctcctttttg gctttattta tttaaaataa aaagaagttt tccggaaatt
    42421 tttatttaac tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc gtgcaaagaa
    42481 agagggagtt tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat gcaagaagaa
    42541 aatttggatt catttttggt taccagtccg tacaatcttc gttacttaac gaactttaca
    42601 ggtacgacag gattagctgt tattactttg gaaaaagcat tttttattac tgattttcgt
    42661 tataccgagc aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa tgtcggacct
    42721 atttttgatg aggtagcaaa cttagtacgc aaagaagaac tgcactcatt agcttttgag
    42781 gaaacaacag tctcattttt agaatattcg gttttagaag aaatcatcga tgcggaatta
    42841 gtacctgttt cacagatgat cgaagaactc agagaagtga aagatgaaga agaaatcgct
    42901 attatcgaaa aagcatgtca tattgcggat atggcatatg atcacatcct gaaaatgatc
    42961 caaccgggca tgactgagat tgaagtcgca aatcaactag acttttatat gcgttctctt
    43021 ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg gattgcgttc ggctatgccg
    43081 catggtgtag caagcaaaaa aatcatcgaa caaggtgact tgatcacatt ggactttggt
    43141 tgctattacg aagggtacgt ttccgatatg acacgtacat tcgctattgg tgatcctggg
    43201 gaaaaattaa aagaaattta tcaaatagtc ttagatgctc aattagcagt tattgatgct
    43261 gcaaaaccag gaatgactgg tgtccaatta gatgcagttg ctagagatta tatttcaaaa
    43321 catggttacg gggaagcatt tggacattct acaggacatg ggatcggttt agagatccac
    43381 gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa tgtgatcact
    43441 gatgaaccag ggatctactt accaggaatc ggtggagtac gtattgagga tgacttgttg
    43501 atcacagaag atggaaatcg tgtgttgact cgttcaccaa aagaattgat catcttatag
    43561 tttcatcaat agaagaagta agcgtaaaaa tcagtgttca cagactttgt tatagaaaat
    43621 cactcgattt aatgatacac tatagaagaa cttttaagct gtaagagata ataaagttgg
    43681 gtgttttctc caattcatag cagtttgcag ctgatcggcg gcagttttct taccacgtga
    43741 tcactatgcc gcatcataga gaattcggat gtatctcgtg cttaggcaaa gggaggaata
    43801 aataaatggc agatgaaaaa aatttgattt taaacgcaaa ccaagattta ggagagatcg
    43861 tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc agcttctaaa gtagaaggcg
    43921 tatacggaat gcgcggaact tttgccagca atgtcactga attattagga cgtgcagctc
    43981 acggaaaagg tgtttactta gcaaacgagg aagatggctt gaaagtagat ctttactgtt
    44041 atctagaata tggtgtttca gttcctaaag tagcaatgga aatgcaggaa cgggtcagcc
    44101 agcaagtcct tttcatgacg gatatcgatt tatctgaagt caatatccat gtagtagctg
    44161 ttgttcctga aaaattagaa caaccaacaa tcgaagaact ttttgaagac gaagaggaag
    44221 agaatgaata aagaattatc aagacacgag attcgggaaa tggcattgca ggctttattt
    44281 ccattagact tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc aatcgaatta
    44341 gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg ttcctgttta tcttgatacg
    44401 ctagttggcg gtgtctgtgc aaaaaaagat gaattggata aagtgattga aaaacattta
    44461 aaaaacaatt ggcacatcaa tcggatctca aaaatggatt tggtgatctt gcgtatcgcg
    44521 atttttgaaa tgacttatgt gacagatgta ccagcggctg ttgctttaaa tgaagcaatc
    44581 gagttggcaa aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg tgttttatcc
    44641 aatgtattga aagaatta
//
LOCUS       DACEKG010000015        43708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEKG010000015 DACEKG010000000
VERSION     DACEKG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.015"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..629)
                     /locus_tag="IW664_000748"
     CDS             complement(105..629)
                     /locus_tag="IW664_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0848131.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW664_000749"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW664_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0848132.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW664_000750"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW664_000750"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0848133.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IW664_000751"
     CDS             complement(2351..3031)
                     /locus_tag="IW664_000751"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0848134.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IW664_000752"
     CDS             3157..4026
                     /locus_tag="IW664_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0848135.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IW664_000753"
     CDS             4052..4567
                     /locus_tag="IW664_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848136.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IW664_000754"
     CDS             complement(4614..5177)
                     /locus_tag="IW664_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0848137.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IW664_000755"
     CDS             complement(5232..5411)
                     /locus_tag="IW664_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848138.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IW664_000756"
     CDS             complement(5488..5997)
                     /locus_tag="IW664_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848139.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IW664_000757"
     CDS             complement(6093..6473)
                     /locus_tag="IW664_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0848140.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IW664_000758"
     CDS             6803..7552
                     /locus_tag="IW664_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0848141.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IW664_000759"
     CDS             complement(7736..9013)
                     /locus_tag="IW664_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848142.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IW664_000760"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IW664_000760"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0848143.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IW664_000761"
     CDS             complement(9935..10879)
                     /locus_tag="IW664_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0848144.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IW664_000762"
     CDS             complement(10881..11567)
                     /locus_tag="IW664_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0848145.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IW664_000763"
     CDS             12041..12361
                     /locus_tag="IW664_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0848146.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IW664_000764"
     CDS             12515..13420
                     /locus_tag="IW664_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0848147.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IW664_000765"
     CDS             13417..14190
                     /locus_tag="IW664_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0848148.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IW664_000766"
     CDS             complement(14371..15795)
                     /locus_tag="IW664_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0848149.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IW664_000767"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IW664_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0848150.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IW664_000768"
     CDS             complement(16517..18499)
                     /locus_tag="IW664_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0848151.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IW664_000769"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IW664_000769"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0848152.1"
                     /translation="MSKINLENLTTERINQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IW664_000770"
     CDS             complement(19412..20473)
                     /locus_tag="IW664_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0848153.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IW664_000771"
     CDS             complement(20684..21211)
                     /locus_tag="IW664_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0848154.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /locus_tag="IW664_000772"
     CDS             complement(21278..22780)
                     /locus_tag="IW664_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0848155.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IW664_000773"
     CDS             complement(23038..24159)
                     /locus_tag="IW664_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0848156.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="IW664_000774"
     CDS             24285..25226
                     /locus_tag="IW664_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0848157.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="IW664_000775"
     CDS             complement(25376..26272)
                     /locus_tag="IW664_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0848158.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="IW664_000776"
     CDS             26541..27881
                     /locus_tag="IW664_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0848159.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="IW664_000777"
     CDS             28099..28542
                     /locus_tag="IW664_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0848160.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IW664_000778"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IW664_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0848161.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="IW664_000779"
     CDS             complement(30867..32090)
                     /locus_tag="IW664_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0848162.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="IW664_000780"
     CDS             complement(32121..32960)
                     /locus_tag="IW664_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0848163.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="IW664_000781"
     CDS             complement(32977..33657)
                     /locus_tag="IW664_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848164.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="IW664_000782"
     CDS             complement(33659..34732)
                     /locus_tag="IW664_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0848165.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35379..36050)
                     /locus_tag="IW664_000783"
     CDS             complement(35379..36050)
                     /locus_tag="IW664_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0848166.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IW664_000784"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IW664_000784"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0848167.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="IW664_000785"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="IW664_000785"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0848168.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="IW664_000786"
     CDS             complement(39933..40799)
                     /locus_tag="IW664_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0848169.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="IW664_000787"
     CDS             complement(40959..43052)
                     /locus_tag="IW664_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0848170.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12620 a         9156 c         7723 g        14209 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaatttaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatgaca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttat gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgttttt
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac
    43681 acggtgttca atcagctgac cttcggta
//
LOCUS       DACEKG010000016        41209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEKG010000016 DACEKG010000000
VERSION     DACEKG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.016"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..328)
                     /locus_tag="IW664_000788"
     CDS             complement(26..328)
                     /locus_tag="IW664_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0848171.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IW664_000789"
     CDS             complement(353..2446)
                     /locus_tag="IW664_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0848172.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IW664_000790"
     CDS             complement(2935..3699)
                     /locus_tag="IW664_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0848173.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW664_000791"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW664_000791"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0848174.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IW664_000792"
     CDS             complement(4704..5780)
                     /locus_tag="IW664_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0848175.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IW664_000793"
     CDS             complement(5804..6397)
                     /locus_tag="IW664_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0848176.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW664_000794"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW664_000794"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0848177.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IW664_000795"
     CDS             complement(8067..8366)
                     /locus_tag="IW664_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0848178.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW664_000796"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW664_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0848179.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IW664_000797"
     CDS             complement(10121..11032)
                     /locus_tag="IW664_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0848180.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IW664_000798"
     CDS             11152..12009
                     /locus_tag="IW664_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0848181.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IW664_000799"
     CDS             complement(12196..12558)
                     /locus_tag="IW664_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848182.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IW664_000800"
     CDS             12904..14343
                     /locus_tag="IW664_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0848183.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IW664_000801"
     CDS             complement(14350..16902)
                     /locus_tag="IW664_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0848184.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IW664_000802"
     CDS             complement(17016..18347)
                     /locus_tag="IW664_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0848185.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IW664_000803"
     CDS             complement(18313..19791)
                     /locus_tag="IW664_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0848186.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW664_000804"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW664_000804"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0848187.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IW664_000805"
     CDS             22401..23828
                     /locus_tag="IW664_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0848188.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IW664_000806"
     CDS             complement(23920..24285)
                     /locus_tag="IW664_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0848189.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IW664_000807"
     CDS             complement(24282..24647)
                     /locus_tag="IW664_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0848190.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IW664_000808"
     CDS             complement(24839..25087)
                     /locus_tag="IW664_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848191.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IW664_000809"
     CDS             25086..25214
                     /locus_tag="IW664_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0848192.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IW664_000810"
     CDS             25271..26965
                     /locus_tag="IW664_000810"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0848193.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="IW664_000811"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="IW664_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27170..27361
                     /locus_tag="IW664_000812"
     CDS             27170..27361
                     /locus_tag="IW664_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848194.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="IW664_000813"
     CDS             27739..28872
                     /locus_tag="IW664_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0848195.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="IW664_000814"
     CDS             28903..29223
                     /locus_tag="IW664_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848196.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="IW664_000815"
     CDS             complement(29252..29716)
                     /locus_tag="IW664_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0848197.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31104)
                     /locus_tag="IW664_000816"
                     /pseudo
     CDS             complement(29847..31104)
                     /locus_tag="IW664_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(31133..31450)
                     /locus_tag="IW664_000817"
     CDS             complement(31133..31450)
                     /locus_tag="IW664_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0848198.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31476..31796)
                     /locus_tag="IW664_000818"
     CDS             complement(31476..31796)
                     /locus_tag="IW664_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0848199.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32260..33675)
                     /locus_tag="IW664_000819"
     CDS             complement(32260..33675)
                     /locus_tag="IW664_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0848200.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33692..35008)
                     /locus_tag="IW664_000820"
     CDS             complement(33692..35008)
                     /locus_tag="IW664_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0848201.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35158..35856)
                     /locus_tag="IW664_000821"
     CDS             complement(35158..35856)
                     /locus_tag="IW664_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0848202.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36145..37134)
                     /locus_tag="IW664_000822"
     CDS             complement(36145..37134)
                     /locus_tag="IW664_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0848203.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="IW664_000823"
     CDS             complement(37214..38047)
                     /gene="msrA"
                     /locus_tag="IW664_000823"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0848204.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38222..39856)
                     /locus_tag="IW664_000824"
     CDS             complement(38222..39856)
                     /locus_tag="IW664_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848205.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39843..41177)
                     /locus_tag="IW664_000825"
     CDS             complement(39843..41177)
                     /locus_tag="IW664_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848206.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12504 a         8475 c         6959 g        13271 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gcaagaaagc
    30841 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
    30901 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
    30961 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
    31021 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
    31081 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
    31141 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
    31201 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
    31261 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
    31321 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
    31381 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
    31441 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
    31501 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
    31561 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
    31621 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
    31681 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
    31741 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
    31801 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
    31861 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
    31921 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
    31981 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
    32041 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
    32101 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
    32161 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
    32221 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
    32281 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
    32341 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    32401 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    32461 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    32521 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    32581 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    32641 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    32701 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    32761 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    32821 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    32881 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    32941 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    33001 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    33061 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    33121 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    33181 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    33241 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    33301 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    33361 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    33421 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    33481 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    33541 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    33601 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    33661 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    33721 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    33781 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    33841 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    33901 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    33961 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    34021 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    34081 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    34141 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    34201 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    34261 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    34321 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    34381 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    34441 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    34501 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    34561 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    34621 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    34681 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    34741 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    34801 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    34861 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    34921 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    34981 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    35041 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    35101 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    35161 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    35221 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    35281 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    35341 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    35401 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    35461 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    35521 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    35581 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    35641 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    35701 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    35761 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    35821 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    35881 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    35941 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    36001 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    36061 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    36121 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    36181 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    36241 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    36301 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    36361 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    36421 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    36481 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    36541 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    36601 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    36661 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    36721 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    36781 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    36841 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    36901 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    36961 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    37021 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    37081 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    37141 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    37201 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    37261 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    37321 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    37381 tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt
    37441 ccgctaattc tttttgtcgt tcgccttgat aaaaaatcac tggacgatat tgcttccctc
    37501 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    37561 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    37621 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    37681 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    37741 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    37801 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    37861 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    37921 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    37981 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    38041 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    38101 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    38161 gagaatcctt atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa
    38221 tttacctttt atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa
    38281 gataagaaat aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt
    38341 tgactccgct tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat
    38401 ttatttctac attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct
    38461 cattagttac tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt
    38521 gactgccaat ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag
    38581 gagaataatc atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt
    38641 tgtttacaac aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag
    38701 aaacagttaa agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct
    38761 tattgtatct tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt
    38821 gcaagcttaa gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat
    38881 accatggcac caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt
    38941 tttctatgtt tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa
    39001 ctagtcccat tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca
    39061 cattagatgc atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca
    39121 tttgttcaaa catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac
    39181 taaataattt tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac
    39241 gaataaaaat aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt
    39301 acactagcaa actcatactc aatgctagct ttacagacgg atacttttct tcttttccag
    39361 agatgatttg ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg
    39421 caattgcgga acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac
    39481 taaataaaaa tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga
    39541 gtgttgtaca caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg
    39601 tcatccaata taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac
    39661 catatccagt ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc
    39721 ccttgattcg agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt
    39781 tgaaaaagat gaaagaatat atactactaa ctataattaa tccactcaat attactactt
    39841 ttttatttat tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc
    39901 aacttattag gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata
    39961 ctgatccata aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt
    40021 tctccagcat ttacatccac attcactaag ccatgttttt tataagagtc atttacgact
    40081 ttaccgttta cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc
    40141 tttttgttgc ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt
    40201 tcccacgatt ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct
    40261 tctgttgggg cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt
    40321 tcttcattga taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt
    40381 gctgctatag acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat
    40441 tttttattca tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt
    40501 tggataccta agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa
    40561 aaccatggaa ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt
    40621 ttttcttttt tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct
    40681 aataacccca tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa
    40741 atagaaagtg cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc
    40801 atctgctgca acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc
    40861 tttactaaat tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa
    40921 cgaactataa tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta
    40981 tacacaagta gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc
    41041 aatccataag cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt
    41101 gcggatcgat aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat
    41161 aaaaaagcag catctatctt tttaaaaaac ttttctccat aataaaaac
//
LOCUS       DACEKG010000017        39960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEKG010000017 DACEKG010000000
VERSION     DACEKG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.017"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="IW664_000826"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="IW664_000826"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0848207.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="IW664_000827"
     CDS             1013..2260
                     /locus_tag="IW664_000827"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0848208.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="IW664_000828"
     CDS             2336..2680
                     /locus_tag="IW664_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848209.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="IW664_000829"
     CDS             complement(2797..3123)
                     /locus_tag="IW664_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848210.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="IW664_000830"
     CDS             complement(3496..4698)
                     /locus_tag="IW664_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0848211.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="IW664_000831"
     CDS             4815..5330
                     /locus_tag="IW664_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0848212.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="IW664_000832"
     CDS             complement(5529..5975)
                     /locus_tag="IW664_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0848213.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr"
                     /locus_tag="IW664_000833"
     CDS             complement(6022..7500)
                     /gene="msr"
                     /locus_tag="IW664_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0848214.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="IW664_000834"
     CDS             complement(7854..8483)
                     /locus_tag="IW664_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0848215.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="IW664_000835"
     CDS             complement(8467..8931)
                     /locus_tag="IW664_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0848216.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="IW664_000836"
     CDS             complement(9418..10686)
                     /locus_tag="IW664_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0848217.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="IW664_000837"
     CDS             complement(10917..11654)
                     /locus_tag="IW664_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0848218.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="IW664_000838"
     CDS             complement(11721..12290)
                     /locus_tag="IW664_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0848219.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="IW664_000839"
     CDS             12418..13290
                     /locus_tag="IW664_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0848220.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="IW664_000840"
     CDS             13632..14504
                     /locus_tag="IW664_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0848221.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /locus_tag="IW664_000841"
     CDS             14501..16459
                     /locus_tag="IW664_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0848222.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="IW664_000842"
     CDS             complement(16710..17561)
                     /locus_tag="IW664_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0848223.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="IW664_000843"
     CDS             17684..18151
                     /locus_tag="IW664_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0848224.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="IW664_000844"
     CDS             complement(18414..18896)
                     /locus_tag="IW664_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0848225.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="IW664_000845"
     CDS             complement(18996..19859)
                     /locus_tag="IW664_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0848226.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20457..21305
                     /locus_tag="IW664_000846"
     CDS             20457..21305
                     /locus_tag="IW664_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848227.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21476..21817)
                     /locus_tag="IW664_000847"
     CDS             complement(21476..21817)
                     /locus_tag="IW664_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0848228.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21903..22610
                     /locus_tag="IW664_000848"
     CDS             21903..22610
                     /locus_tag="IW664_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0848229.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22597..22920
                     /locus_tag="IW664_000849"
     CDS             22597..22920
                     /locus_tag="IW664_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0848230.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23009..24046)
                     /locus_tag="IW664_000850"
     CDS             complement(23009..24046)
                     /locus_tag="IW664_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0848231.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24493..25476)
                     /locus_tag="IW664_000851"
     CDS             complement(24493..25476)
                     /locus_tag="IW664_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0848232.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25892..26662)
                     /locus_tag="IW664_000852"
     CDS             complement(25892..26662)
                     /locus_tag="IW664_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848233.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26655..27590)
                     /locus_tag="IW664_000853"
     CDS             complement(26655..27590)
                     /locus_tag="IW664_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848234.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28184..29833)
                     /locus_tag="IW664_000854"
     CDS             complement(28184..29833)
                     /locus_tag="IW664_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0848235.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30028..30924)
                     /locus_tag="IW664_000855"
     CDS             complement(30028..30924)
                     /locus_tag="IW664_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0848236.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30917..31597)
                     /locus_tag="IW664_000856"
     CDS             complement(30917..31597)
                     /locus_tag="IW664_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0848237.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31598..32380)
                     /locus_tag="IW664_000857"
     CDS             complement(31598..32380)
                     /locus_tag="IW664_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0848238.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32373..33290)
                     /locus_tag="IW664_000858"
     CDS             complement(32373..33290)
                     /locus_tag="IW664_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0848239.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33293..33577)
                     /locus_tag="IW664_000859"
     CDS             complement(33293..33577)
                     /locus_tag="IW664_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0848240.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            34365..37592
                     /gene="fss3"
                     /locus_tag="IW664_000860"
     CDS             34365..37592
                     /gene="fss3"
                     /locus_tag="IW664_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0848241.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            37705..38019
                     /locus_tag="IW664_000861"
     CDS             37705..38019
                     /locus_tag="IW664_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0848242.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            38032..38406
                     /locus_tag="IW664_000862"
     CDS             38032..38406
                     /locus_tag="IW664_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0848243.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            38416..38751
                     /locus_tag="IW664_000863"
     CDS             38416..38751
                     /locus_tag="IW664_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0848244.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            38834..>39960
                     /locus_tag="IW664_000864"
     CDS             38834..>39960
                     /locus_tag="IW664_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0848245.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
BASE COUNT        12156 a         8143 c         7384 g        12277 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatatactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctactg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt
    20221 atttatttct aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc
    20281 atctttaaaa cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga
    20341 atgaattttt gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt
    20401 tcttaaacgt ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga
    20461 ataattggct tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc
    20521 acggttttgt ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca
    20581 cggaagatta taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc
    20641 tatatgccat agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag
    20701 aacgtccaat aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg
    20761 ctagttcctc tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcacttta
    20821 tcgaacgctt caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa
    20881 ctatcatcca taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt
    20941 tcatcatgga gatgcgaaag ttcatccaag aagcagttga atcgcttatt cagcaaaatg
    21001 cctatcttcc cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt
    21061 gggagcctga ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag
    21121 aattattagc agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc
    21181 aagtcgatca tactgacgcc tcttattcaa aacgctactc tataaaaatc ctgaaagaag
    21241 actttcggga aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa ctaaagaaac
    21301 ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac gataacaacc atcctaaatc
    21361 cacaagaaat acagcagacc ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag
    21421 aagacttaac gcgtacccaa tattagtcat ttgactatta ggtacgcgtt ttgttttata
    21481 agtcatgaca aatttccttc gttgctggat agattttccg gcattgttga tagattttat
    21541 gatacttttc tacattttgt aagagaggtt ccgctgcatc tgtatattgg ataaaattct
    21601 gacaacaact ttcaggtgtg tcgaaccacc ctaaccctag tgcagcaatc attgttgcgc
    21661 ctaatgcagg tccttcctcc acacttaaac aatagaccgt tgcatcgaaa atatcagctt
    21721 ggatttgtag ccatgcaaga tttttcgcgc ctcctcccac cgaaacaata cgtgtaaatc
    21781 tgcgacccgc tagttcttta cgtttcaatc ccaccattga caaatgctca ttaaaaattt
    21841 agaatattca caagtttaac gacaattatg aaaatttatg tcgtgctaaa ggagttttgt
    21901 tattggatga aaagttagat ataaaaactg cgatcaaaga cacgttacct accgttttcg
    21961 gttatatcgg tattggactt gcatttggta tcgttgggaa agctgccgga tttcatccac
    22021 tagtcgtcac gttgatgtcc ctgctggtct atgctggttc tgcccaattt atcacagtca
    22081 gcatgcttgc tagtcacagc ccattgcttt ccatcgtttt ctcgaccttt ctagtcaatt
    22141 cccgaatgat tctgatgagt atgacgattg ctccttattt caagaaaaat cggctgcttc
    22201 aaaatctgct gattggcaca ttgctgacag atgaaagttt cgcactggga atgaataaat
    22261 tgaactatac tggacaaaag ttaaactttc gttggatgaa tacagctaat tggatttctt
    22321 atttgacgtg ggtgggttct tctcttgttg gcgcactttt agggaatttt attactgatc
    22381 cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt tatcgggtta ctttatcttc
    22441 aagtcatttc tgatagaaac acctctaaac gattacagtt gatcctgatt ggtctcactt
    22501 tgattctggt atatgtcgga ctgattttta tcccaagcaa cttagtgatt gtagttgtaa
    22561 cactgattgg gtgcggattg ggggtgtgga tcaaacatgc cttcttttga gtttacattg
    22621 ctgactattt taggttgtac tttagcaact tggctttcta gggtcctacc atttgtctta
    22681 ctgaaaaaat tcgatttacc gcaaccgttg cttgagtatt taagttttgt cccgatcgtc
    22741 attatgtctg ctttatggtt cagcagtcta tttacccaaa atatcggaca tctgccgcag
    22801 atcaatctcg aaaatgcttt ggcttcagtt cctacacttt tagctgctat tctttcaaaa
    22861 agcttattgg tgatcgtctt ggcaggaatt ctttcccttt cgttgattcg cttattataa
    22921 ataaaaaaac aagagcttat ggcaaattac tgtcatgcgc tcttgttttt atcaaagtca
    22981 aataagaaaa aaatcggcgt attcttgctt atccgttgaa cggaccacta acaacgactt
    23041 ttcctaacat atgtcctgtt tctacgtctt tggttgcttg tttcaagttc ttcgcattca
    23101 tcccatctga atagaccttg ctcaatgtac tgcggataat cccttcattt gctaaagcag
    23161 caatgtgcgc taaagcttct ccttgcgttt cgatttcata gttatagtct gttttagcga
    23221 acatatactc ccagtcgaag ctgatagatt ggtttttcca aacacctata tctaacggct
    23281 ctttgatccc tacgatcgtt cccacatgtc caaatggtcg gacaaggtgc ttgatttgat
    23341 caaagtagcg ggtaatatcg aataatacag cgacatactc tacttgatcg attcctaatt
    23401 cacgaaaact tttacttaag tcgtgatgat aatcgattgg atagtctaca cctgtttttt
    23461 tcagccattc aaaattcttc ggacttgcag tcgcgtagac ttctaggcca gcccaatgcg
    23521 ctagttgatt caaaatcgaa ccgacgcccc cggatccgtt gatcactaag atttttttcc
    23581 cgcgattggc attttcttct ggagttagac caaatttttc aaataacaat tcataagcgg
    23641 tcaatgaggt caacggcaag gctgcagctt cttcatctga aagatttttt ggtgcaagag
    23701 cgacaatacg ttcgtcaact agttggtatt cttgattgct tcctgctctt gttgttgtac
    23761 ctgcgtaaaa aacgcgatcg ccgacagaaa atttttgcac ttcgtcacct acagcaacaa
    23821 ctttcccgac tccatcaaaa cctaagatac gtaaagcatt tcttatcccg ttatcttggc
    23881 gaagtttcgt atcgacagga ttaacagata ctgcgttgac ttttaccaga agatcccgtg
    23941 aactgggtac aggtatctgt tctttttcat caataaagct ttttggatca tcgatcggca
    24001 acccttcata aaaaccgact accttcatta attccttttc tgacatacga attcctcctt
    24061 agcttcatca agttggagca tccatacagt gttcatttca atatatatag tatgctattt
    24121 gttccacctt ttttattcat cataattatt ctcttactta aagtaaacta tttgcacttt
    24181 tttggttaaa aaagaggttg cgacaagact tttgtcacaa cctcttataa acggtgttct
    24241 cctaccaact cttcggtcac aagtcaaaat attcatcaaa catgagaagc tgttttttga
    24301 aattgctcct tgtgactcag agttaaacgg caggctcaca acctctgata taaacggtgt
    24361 tcaatcaact gaccctcggt attagctcaa aaaacgacaa aatatgaaga gctatttttg
    24421 tttggctgtt cttattactt gatacaggac agctttttca cgacctctaa ttcacggtgt
    24481 tcaagcaaga aatcattgtt cactgaaaag cgttggatgt tctttttcaa aggtctctaa
    24541 gatctctcgt acttcatcag tagatttcgt attcatcaac tgaactcgca agtcatttgc
    24601 tcctggaaat cctttgatat agattttaaa gaaacgatgt agtcctgtga tggaacgcgg
    24661 gatgatttct gaataatgat cttgcagatc tagttgcaga cgaagtagtc cgatcagttc
    24721 tttttctgta tgttccctcg ggtctttttc aaatgcataa ggattcttga aaatccctcg
    24781 gccaatcatg atgccatcaa cgccatattt ctcggccaat tcttctcctg tctggcgatc
    24841 ggggatatct ccattgatcg tgattagtgt ttgtggagcg atttggtcac gtaaagcaat
    24901 gattttagga atgacttccc aatgtgcatc tactttactc atctctttac gcgtacgcaa
    24961 atggacagaa agattggcta tatcttgatg aagtaggtga ctgatccatt cttccatctc
    25021 agacatttcc gtatagccga tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc
    25081 tgcttcaatc aattgtgccg ctacttctgg acgaaggatc aagccactgc cttttccacg
    25141 ttctgctaca ttagggactg ggcagcccat gttgatatcg attccttgga agcccatttc
    25201 tgccatagca atgctcattt ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac
    25261 catcggctgt tcatcttctg taaatgttaa tcttcctcgt acactgtctt ttccatctgg
    25321 atgacagaaa ctgtctgagt ttgtaaactc agtaaagaag acatccggtg ctgctgcatg
    25381 ctttacgaca tggcgaaata caacatctgt tacatcttcc attggtgcta aaacaaaaaa
    25441 aggctttggc agctctttcc aaaaattagt agtcatttat ttgttactct ccattctatc
    25501 aagcagtgaa gtcgcaatct ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag
    25561 aagcaaagtg aacagcttaa aagatgtttc tgattgagca aaaaaaagac tgtgacaaaa
    25621 atcttatcac agtccatttt ccggaataaa tggtgttcaa aagcttgcat ttgcggtaat
    25681 aagtccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga
    25741 tgcagaacag ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt
    25801 attcgctcaa aaaacaacaa aatatgagga actattttcc cgtttgtttc tctaatactc
    25861 aggtcaaaac gctgatttca caacctcttt tctaacttct caatcctctt cctttattga
    25921 tcaaatacca agtaacggcg tacaaaacaa cgatgaataa cacaagtacg atcatcgatg
    25981 ttgctactgg cacatcgatc gttccaagga atccgtaacg gaaaccggag atcatataaa
    26041 cgatcggatt gacttttgaa acagcttgcc aaaagggcgg taacatagag atggcataga
    26101 aaacgccacc tagataagtt aacggctgca agacaaatgt aggcacgatc gatacatcat
    26161 cataagactg agcaaaaatc ccgttgagca gtcctgctaa tgaaaacaag attgccgtca
    26221 tcaataatgt aatgatgacc atgaaccatg aatacacatg aagtggtaca aagaacaagg
    26281 aaatgatcgt tacgagagag ccgacgagaa tactgcgacc cacaccacca atcacaaagc
    26341 cccaaataat cacatgcgta ggaacaggtg ctaccaagat ttcttcgata tttttctgaa
    26401 atttttgaga gaagaaggac gaagaaacat tggcatacga actagtgata gcagacatca
    26461 taatcaaacc aggaacgatg aattccatat atgaaaaacc acccatgtca ccgatccgcc
    26521 caccgatcat ttttccgaaa ataacaaaat acaaggatgt cgtgatcact ggcggaacca
    26581 gtgtttggac ccatatacgc aaatagcggt tcgtttcctt gatagctaaa ctttttaatg
    26641 cagtaaaata aagattaaac atttttctcc tccgtatgat tattttcttc tgtgattttc
    26701 aagaacagtt cttctaaacg attcgactta ttcctcatag aaagaacttt gattccttgt
    26761 cttgtaagct gatcgaagat tccgttcacc ccttgatttc gttcaacttc gacagcgatt
    26821 gtctgctcat cttcaaatgt acaagcatat ccttcaatca caggtttggt atcatatggc
    26881 gctagatcaa agataaatgt ttcaaattgc agttttgaca gaagctcttt catacttgta
    26941 ttctcgatca gttcacccga ttggatgatt ccgatgttgc gacaaagcat ttcagcttct
    27001 tctagataat gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc tcgtagaaac
    27061 tcccacatct cgcgtctcaa ctcgatgtca actcctgctg ttggttcatc caagatcagc
    27121 aaacgtggtt catgcatcaa tgccctagca atcatcagtc ttcgcttcat tcccccggaa
    27181 agcatacgtg cacggacatt tctcttttcc caaagattgg actgcttcaa atatttttcg
    27241 ctgcgtttca tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt cacgacgatt
    27301 tgctggaccg tttcaaaagg gttaaaattg aactcttgtg gaaccaatcc aatctgttgc
    27361 tttgctcgaa ctagatcctt gtccagatca taaccgaaga cattcacttt tccagatgtt
    27421 ttattgacta aagaagtaat aatcccgatc gttgtagact tcccagctcc atttggccct
    27481 aatagcgcat agaaatctcc ttctttcacc gaaagatcga ttccccgcaa tgcttcgacc
    27541 ccacttccat agatcttttt caaattattt atttcaagcg catttgccat ttatttccta
    27601 tctccttata gacatgattc ttaattgtat catggaatac agcagatgaa aaaacaactt
    27661 ggcttccaca caaaaacaca cctgctaaca aagaggagat gtgttttcta gttcatgatc
    27721 atcttcgata gaataactat ctaaacaact ctggaacttt taaaatcata aaagtaccga
    27781 cagcaactac tacgatgaca tatagaagat ccttataact gaggttcttg tcttttttat
    27841 tcatgttaag ctttcctccg tccttttatc gaaagctttt ttcttttatt ttaaccgaaa
    27901 taaataaaat ggaaatagtt attttcaatt tctgttttcg ctgtttgttt tacggagaac
    27961 tttgccatgg aacgtgcgtc tcgtcgctga tgctaagttt tttcgataaa agctgctaga
    28021 aggaagacag cgtggtttat tttgtatcgt cttttgctaa atcttccaag atcatctgga
    28081 cagcagactc gttcccattg aattctgcca cttttttggc atcaatgtac ttcttttgat
    28141 aatcgtcttt ttggacccac gcatcatgct tcgtttgata aagttaatca aacggatacc
    28201 actgacgctg ttcatcgaac aacaaacttt ctttttgggc ctgttcaact atggatttag
    28261 gataattcaa aactttcagc aaggcaatcg catttcgggt ccttgaaggt ccttctttta
    28321 atttgaaatc aaaactgatt ccttttcctt ctgttacttg ttcttcaaag tggacattct
    28381 cacaataatt ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag
    28441 atggatactc cgcaagccac cgaacgactg aagcggaaga agcaatccgt tcgatcgtat
    28501 ttgttccttt taaaatctca tctacgaagc agtagcagcg ttctttggaa gccacttggt
    28561 ccagcaaacg tttgatcgat ttgatttctg aaacaaaata actgtcgcct tcaaataaat
    28621 catcttcgat tgccatggaa gtcaacacat gtccaggttg cagggtaaac tgttctgcaa
    28681 tcgctgtttg gatcgtctgt gctagaatac agctgatggc tatacttttg acatacgtag
    28741 attttcctga tgcattggaa cccgtgacta atgtattctt ctgccagttg actggattaa
    28801 ctaccgcttc ttttaacaat ggatgataga tatctttcgc tgtcacaccg ccctcagtaa
    28861 actctggtcg gcaagtgatc ggcatatagg tgcgataatt caaaacggct gctgcaactt
    28921 ccaattctcc cagcaattcc cacagcttga tggcttcagt tgaatgcttt tctaatttag
    28981 tgagaacgaa atgataggag ataaatggca gcatgaacat gatgttgaca tattcaaaaa
    29041 tcaattctgc ttcactcccg ctcttcattc gaaatgaaaa acctaattta gggatgtctc
    29101 ttaatggttt agtttgctgt ttcagctctt tttgaagtgg tgtcgtcatt tttgctaact
    29161 gattggcggc tgaaatcgtt gaaaccaaat aactcatgct attcagctct gtttctagtg
    29221 tttgtttctt cactagataa tagaccgtat tgaatactaa gccaccgatt gctagataga
    29281 ttcctagtac ttgccctaaa agaagcaaac ctacacccac cagcggaagc aagccaagaa
    29341 aaataaataa acctacgcgt ccaatctttt tactttttcc atccgctaga tattgttttg
    29401 caaaattatg atcttttttt cctaaacgag cgaattgata ttggattctt tctctcagtt
    29461 gaggattttc ctgataaaaa gcaattagtt tttctaactg ataatcttct ccaaaatcaa
    29521 agctacgtaa acgctgatac aacgcttctg aacctacact agaataggta ctgttgatcc
    29581 cttcaaatac ttcgaacata tccaagtcat accaagtaag atcgtcgatt tcactatccc
    29641 atgaacggaa ttttttctcc gtgagccacg catccttcaa actttcttct ttatcaaatc
    29701 tcggttgata aggaattttt ccccaattac tacgaactgt ttcctttact ttcaaacgtg
    29761 aaaaaatgtc taaaacaact aataaaataa caactgcaat aataattaaa acgataaatt
    29821 ctctttggcc catatccctc tccgttcctt gatttgttag ttttagtata gcactgttgc
    29881 gctatttgaa aagggggtac aaaaataact gtcttcgtta cctaattcgt atcaatcaca
    29941 ccaaaataaa aacaccttcc aaaaaagaag atgtctttaa aacgagatac aattttttgt
    30001 cattcacttc aggtaaatta gagccaatta aaccctccgc aacttgacgg agatggagaa
    30061 gatgcccccg tctaatctag cggtcatctc tcctcccatt ccttccacca atttttttga
    30121 caggtaaagc ccgattccgc tagattccgt atttgtgcgg ctggtatttt cggtataaaa
    30181 acgctggaag actttttcaa cagaggtttg cgctcctggt ttgataggat tggccactgt
    30241 taatcgaatg aatgaatcct cttttgaaaa tgtcaccttt gcttgtccat ctgcataacg
    30301 caataaattc tgtatgatat tttgcagcac tcgcagcaac atgagcggat ctgctaatac
    30361 agttgtattt atcgagtgtt cagcgaacgc tacctcgata tttttctcag aaaattgttc
    30421 ataaaaagag aaaaatactt cttccacctc tttagaaaaa gaaagcgtat ggagatccat
    30481 ttctttttgc ttcgtctcta ttcgagcaag atcataaaac tgatccgtca tactgagtaa
    30541 tcttgcaaca gattgtttga tgatcgttaa atacttctcc cgtttctccg tatcctctac
    30601 ctgttccagt aattgtacat aaccgttaat agcagtcaaa ggagttctga gatcatgaga
    30661 aaggccgacg atcgatagtc tgatgttttc ttccatttct tgaaatgctt gtcctttttg
    30721 ttcattttca tccacccatg tattcagtgc atctactagg tccattagat tcttttctcg
    30781 aaattcgact gaaagacgac caccatattc ttgtcgttca gaaagagtgg cgattttctt
    30841 agttaatctc ttgatttccc tttgcaggaa gaaaataaaa cttcccataa agataacaac
    30901 gagaagcaac agccacttaa tcaaatctaa accccactcc ccacaccgta tgaatataca
    30961 cagcatgtgc ctttgctagt ttccctcgaa gattggaaat atgcatattc actgttttct
    31021 cactatcaaa atatggttct tgccaaactg tttcataaag atttgctcgc gaaaatactt
    31081 ttttcggatg ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc aaatggacag
    31141 ccacaccatt cacatttaca tccctcgact ctttgtccag taagatttcc ttgtaataga
    31201 tgcgttgcga cagatctttt acttggacag aagtctgatg acgaagttgg atgccgattc
    31261 gtaacaacag ttcttcgata tcaaatggtt tcgtcacata gtcatccgct cctgctgcca
    31321 acaaggaaac tttatctctt tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg
    31381 atgtctgtcg gatttctgct aacaatgctt cacctgaaag ccctggaagc atgagatcta
    31441 gaaggatcaa atcgacagga tatgtgctta acaataattt tccttccgta cctgaataag
    31501 catcaagaag tttgtatctc tgttcaagaa cctcctccag taaagaatga acagcttcat
    31561 cgtcttcgat aatcaaaata gtttccagca ggaacaccta cttaatgtca gatttttgga
    31621 aaataaacat tcctagagct gatgatacca cgattgttac tagactgacc agaatcattt
    31681 tcggtgcttg cgaataagtt gcttgttgcg caaagtccgt atacatgagc cacgtttccg
    31741 taaaccactc cgttagaaat tccaagcctt tgatttttgc gaaaacaccg ggaacgaagg
    31801 ccgttcccat gatataaaga aaagaaagaa gcaaactgac actggtactt ctagtgatga
    31861 atgtcaaagc cactggaaca gataatgtcg ctaatatcag cagaagcaat acaggaaata
    31921 cttttagaac ataatttcca tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa
    31981 gtgtgtaagc aatcatggct agccccgtaa tcaaaaccac gccaagtgtt gtgatcaaaa
    32041 gcgtaacgag aagtttcgac acataataag tcattcgatt ccgtcctaaa gataacgtat
    32101 tgcgaatcgt accgttttga aattcttccc caatataaaa gcacgcaaat gctgcgaaag
    32161 ccagaagaat ccatgcagcc attttttcca ccattaaaat ccctgttgca ccgttgatga
    32221 ctgtatccgt tgcttggttt aattcattgc cgtattttgt atgcatatac cattcgttcc
    32281 aagaataaaa aagcggaagt acgaccatca gtcctagtga gacccacatt ttacggctct
    32341 tccatacttt tattttttca gcagttaata gattaaacat gtggattgcc tccagttaat
    32401 tgtaagaaat aatcttccag tttttgccca actaggtgga taccatccac aggaactttt
    32461 gcctctacca atgcttgatt gatggctgct acttggtcaa ttccttcaaa aagctggata
    32521 acacggctag attggacaaa ataatcccta taaccaagag agtcgagaac cgttactgct
    32581 tgttccgtcg ccgaagtgtc aatttttata aattgacgag attcttgtgc caattcttct
    32641 ttcgataatt ctttgatcaa gtgtccttca tgcaaaatac cgtagtgagt ggcgatctgt
    32701 gacaattcat ctagcaagtg gctagaaagc aagacggtga tgccccgttc ttttaccaaa
    32761 cgttggatga tttctcgcat ttctgccatc cctgctggat ccatcccatt cgtcggttca
    32821 tccaaaatca aaaattctgg attcgtgatc agtgcatttg caatagctaa acgctggcgc
    32881 atacctaaag agaaattctt tgctttcttt ttccctgtat tttccaatcg catcattttc
    32941 agcaggtctt cgatttctct atccgacaca ccgccgttcg ctgcttggat acgtaggtta
    33001 tctcttgcag ttaattctgg ataaagagca ggggtttcaa tcgattgacc catacgtcgt
    33061 cttgctgcct gcaaatccgt cgttccaaat aattcgatcg aaccttcatc aatcgagatc
    33121 aaacccataa tagttctcat aaaggtagac ttacccgctc cattctcccc aatcaaacca
    33181 tagatcattc cacgtttgat ttcgatcgat actttatcta atgctttggc agcaccgtat
    33241 tttttggtta taccagttgc tttcaagacc gtttcatttc caaaatccat acttatcctt
    33301 ctttcttatt gataagtgaa gtatagttga cgggtataaa gaaagcagcc agaaatgtat
    33361 aaagaaagtg taaattttcc aattgaattt caagaaaaat cagactgtct cgcagacgtg
    33421 gttgagttgt ctgccaatca aagagcgcac caaacagtgc taatccaagt ggactggcca
    33481 ccatggtaat cgttgttatc gttgacatca gacgtccttg tatatgcgtt ggaacttctc
    33541 gctgaagcca gaccatataa aaagcaaaag aaaacattaa tccttaaaaa cggacttatg
    33601 aaaaataata catgccgttt gggatttaca attagctacc aaataagagg ttatgacaaa
    33661 agacttttca caatcccttc ttccgaataa acggtgttct cctgccaact cctcggtcac
    33721 aagtcacaat atttatcaaa catgagaagc aatttttgta aattccttct tatttcttga
    33781 agctgaccac ttctgtcaca acctcagaag cggataaata atattgtttg atgatacttt
    33841 atctttttcc cgtttgttta tagatcgaat gatattatag accaagaatc ctacctatta
    33901 acatagctaa aaacacaaaa aaggcacttt ccaaattgtg gaaagtgcca gaaaagttag
    33961 gattaaggta actgcgctca gcattgcttc gcccagtacg attctcgtaa ctcgtgaact
    34021 cgaacgataa tcgcaattaa cgttttgaga actgtgaagc tttacgggct ttcttaagac
    34081 ctggtttttt cttcgtatca ttgaataagt ttgaaaaacc ttgatttata aaggttttcc
    34141 cgtaaatata ttttattata tttgattaaa tttgaaatgt ttgtgttcta aaatgtgttc
    34201 taatttaaag gcagtatatt tcgtatttta gattaaattt ttaggctcat tcattctcaa
    34261 atccattcta ttcagtcaat aaaaatttta aaaagacttt ataaacaaag atgttcaagc
    34321 gagcatctta tttttttgtg cttaatttga aaggagaaca tttcatgtat aaaaaacaaa
    34381 aaaagaaatt atgtgtatta actattttga gtttggctgt tcaagcaatt gttggagcaa
    34441 ttgctcctac gattactttt gctgatgaaa ttacccatcc acaaacggta accgtagaac
    34501 tagatttagc acatcaatat gctgttgaag gaacttttag tgatggtcgt ccaatgtcag
    34561 aagtcactgt tccgcattat gcagtgtaca acggctttaa acaagatatt ttctgtattg
    34621 aaccaggagt cccgatttac aatgagttca ctcctggcta tgagaaaaac ccgttaccag
    34681 atatgtccga taaagccaaa ttagtttccg ttttatggaa aaaagtaggt acagatgcag
    34741 atactcatat tgtggcacag aagatgatct ggcaagaagt taatgggtat acccttcact
    34801 caataaaacg atcagatggt agtgccgtaa atatcgcagc cattgaggca aaaataaatc
    34861 aagccattga cgattatcag aagaaaccaa ccttccataa tagtacggct aaaacagtat
    34921 tagggcaatc gactactgtg acggatacga ataatctgaa tttgtcagag ttcgatgagg
    34981 ttgtggaaaa tgccgcaaac attgattatc gtgtgaatgg taatcagttg ataatcaccc
    35041 caaatgccaa ttcaaaagaa agtggtgtgc tgacgcttaa gaaatctgct ggtaccggaa
    35101 cacctgtcgc ctataaaaag gctggccaac agactttaat ggccggggcg attgataaac
    35161 ctaataccta cacaatcaaa attgatgtgg aaacggaggg ttctttaaag atcaaaaaag
    35221 tcgataaaga atcgggtgat gttgtaccag gaacggtttt ccatttagac tttggaaaaa
    35281 ctttacctgc aaaagacgtg actactgaca aagaaggcat tgctacattg gatgagattc
    35341 ctcatggtac aaaagtaacc attaccgaaa aatcagtacc ggcgccttat acgattgata
    35401 ccacccccat gactaccacc attaaagcag gcgaaaccat ttacgtgact tcaaaaaacg
    35461 cacgggaaaa agggcaaatc attctggata aaagcggagt tgaaacaggg agtgatcttt
    35521 ggaatgacaa ttattcctta gctggtaata cgtttgccat tcgcaaagac agtccaaccg
    35581 gtgaaatcgt tcaagaaatg accacggatg aaaacggtca tgcggaaaca ccaaaagaga
    35641 ttgctaatgc gttggaattg ggaacctatt atgtgaccga aactaaagct agtcatgggt
    35701 tcgtgaatac gttcaaacct gtaaaagtcg aattgaagta tgccaatcaa accgtagccc
    35761 ttgttaccag taacgtaaaa gggcaaaatc aagaagtcac tggcgaaacc actttgacga
    35821 aagaagacaa agatactggg gataaagcac aaggtaaggc agtctttgag ggtgctgaat
    35881 acaccttgtt tactgctaag gatggaaaag ctgttaaatg gagtgaggct tttaaaccag
    35941 agttagcgaa gggaacgaaa ggttctgatg aaacggtcac tttgacttta gacgaaaaga
    36001 accaagttgc cgttaaacat ttagctatca acgagtatta ttggcaagaa acaaaagcgc
    36061 ccgaaggata tagccttgat gaaacaaagt atcctgtatc catcaaaaag gtggatgata
    36121 gcgaaaaaaa tgccgtgatt acccgaaatg ttacggcaaa agaacaagtg attcgctttg
    36181 gctttgattt ctttaaattt gctggatcag ctgcgggcac taccgaaact ggcttcaatg
    36241 atctgacctt taaagtgtcg ccattggaag ggaccaatga aatcacaggt gccgaagatg
    36301 aagcgaccac agcttataac gagcaattag ggtttgatgg ttatggcaag tttgaaaatc
    36361 ttccttatgg ggattattta cttgaagaag tagaagcccc agaaggtttt caaaagatta
    36421 cgccattaga aatccgttct acttttaagg aaaacaaaga agactttgtg aagagtgagt
    36481 atgtctttac cattaccgaa caagaccaaa aacaaccaat taaaacggtg accgttcctt
    36541 acgagaagct gacgaacaaa gcattttctg ttagtttgaa ccgtttaatg ctctatgatt
    36601 tgcctgagga agaagatagt ttaacttctc ttgcgacttg gaaagacgga aacaaagaat
    36661 taacttctct ggattctacc gagctagttg ataaattgag ttacaatcta catgaaatca
    36721 aagaagactg gtatgtggta gcgcaagcca ttgatgtgga tgcgacaaaa gctgcccaag
    36781 aaaaagatga aaaagccaaa ccagtggtga ttgcagaaac aagcgcaacg ttggcgaaca
    36841 aagagaaaac aggaacttgg aaaattcagc ataaattaac cgccgaacaa gttttaaata
    36901 aaactattgt cttattcaac tatgtgtatg aaaataagga agcttttgaa gcaggcgata
    36961 agccagtagc gaaggatgtt agcttgaata accaagccca aactgtcagt tgtacagtgg
    37021 aacaccatgt ttctatccaa acaaaggccc acctagaaaa cggttctcag acctttactc
    37081 atggtgatgt ggtggatatg tttgatgatg tgtcgattac tcatgatgta ctggatggct
    37141 caaaagaagc tttcgaaaca attctgtatg ctttattacc ggatggtaca aacaaagaaa
    37201 tttggaaatc tggcaagatt gattatgaag taaatgataa agaattcacc aaaactgtgc
    37261 ttgcgaaaaa agtagatacc ggaaaatatc cagaaggaac caagtttact tttgcggaaa
    37321 tcaattacga taaagatggg aatatcaatg gaaaacacaa tgaagatttg aaagaaaaat
    37381 ctcaaacctt aacaccaaaa gaagttccaa ccatactaag tacgccgaaa caaccggaaa
    37441 caccaactgt tccaagtggc tctcaagaat ctagtcccac agtgaagacc ttccctcaaa
    37501 ccggggagaa aaattccaat gttttactgt tcattggttt tactttgatt tttgcgacgg
    37561 cgggctatta tttctggaat cgccggaact aaggtgatga gatgatacaa gtaaatccta
    37621 agaaccaacg tgcgcgaccg cctcctaaaa aagtagtcgc gctatttttg tatccaaaac
    37681 taacaatgaa aagaggaaaa gaagatggaa ttgaaatttg tcgtgccaga tatggctgaa
    37741 acctttggaa agttaagtta tgccggtgaa ggagaaatta taacagaagg atatggacgg
    37801 aatactacgg tgattggtcg cagttatcat ttgtattcca gtaagcaacg ggccgatgat
    37861 attgaggtgg tagtagctgc ggaagccggt gaaaaggatt ttgatcaaga ccaaccgcta
    37921 aaagccgtga atccccattt ggttgccaaa ggctatgaga ttgagaatcg tgggtttact
    37981 gattacgtgt tgtatgttga tgatttagtg aaagcatagg aggaaaagaa gatgagatta
    38041 acagaaggta ttgtcgtaga ttcaggttta acatttggga agttgcgttt ttccgcatta
    38101 cgccgagaag tacggaagca aaatgaggat ggaacaattg gtaatgaggt aaaagaacga
    38161 acctataatt taaaatcctc tgcacaaggg cgaatgattc aagttagtat cccagctaat
    38221 gtacccttac gcgagtttgc ttatgatgca gaggtagagt tggtcaatcc aatcgtggat
    38281 accgttgcca attatgtttt tcgagaagga actaccgtta attggttcat taaggcggat
    38341 gatttagtct tgaagcggca gccgaatcaa ggaaatccta cgaaccaaaa tgaaagaaag
    38401 aaataggtga agattatgga aattaatgtt gaacatattt tagattgtct cgatcaatat
    38461 ggaaaaggcg agctgactga ggaacaactc gttaaagcat taacctctga tgaaaaaatg
    38521 tttctgatta tacatcaagg tttacttgga gaaaatagcg acagtgaaga agactctgat
    38581 gtactagact ggttaggaga agaagagtct ttctttatag ttgtcgaagt aaatgaaaac
    38641 ttatgtcggc aggcagaatc cattttggaa gaaattggtg tggagatgcc ggatgctatt
    38701 gaaggctttt taaatcaatt ggttgaaacg aaacaactcc cctcctcgta gcagtaaatg
    38761 actagcataa ctttttgaaa ttgcctaatg aaatcctatt gggctttttc gattggaggt
    38821 ggaaaccatc aaaatgtaca aaggacatcg catacgcgca ggagatcagc acttggttta
    38881 tcactttgtt cttggctggc tactcgctct gttcatcggt tggatgagcg tcttttattt
    38941 tcaagagttt agacaatttg atatttctaa gctatcactt tctactattg aaatcgtttg
    39001 gtctataaaa gatttagtat gtctgcttgg aagtctcgct ttttcaggag ctatgatatt
    39061 gctttatatt cacttttttc ttgatcattg gcgaagcttg tggcatcgac aaaagctagc
    39121 tcggatgatt ctagagaacc attggtatga agtgaaacaa actcaaagtg aaggcttttt
    39181 caaagatttg aatagtagcc gaaccagaga gactatcagt tacttcccca aaatctatta
    39241 tcgaatgaaa gatggattac tgtctattcg cgttcaaatt tcacttggga aataccaaga
    39301 tcagctttta aaattggaaa agaagttaga aagcggactg tattgtgagt tagtagaaaa
    39361 ggaactcaaa gactcctatg tggagtatac tttactgtat gatatgatcg ccaatcgaat
    39421 tgggatagac gaagtagtgg cagaaaatgg gactctgcga ttgatgaaaa atcaagtatg
    39481 ggcttatgat tctttacccc atatgttaat cgctggtggc acgggtggag ggaagaccta
    39541 tttcctcctc accattatcg aagcactatt gaagtcagat gcagaattgt ttatcctcga
    39601 tcccaaaaat gcagatttag ctgatttagg tacggtgatg cctcatgttt attcacaaaa
    39661 ggaagaaatt tctgcttgtg tggaagattt ctatgaacgt atgatggctc gtagcaaggc
    39721 aatgaaagaa atgtctaact acaaaacagg agagaattat gcctatcttg gacttccacc
    39781 caacttttta atctttgatg aatacgtggc ttatatggag atgttaacga cgaaagaaag
    39841 tgcggtgatt ttgaataagc tcaaacaaat cgtgatgttg ggtcgtcaat ctggtttctt
    39901 tctgattttg gcttgccaaa ggccggatgc gaaatattta ggagacggaa tccgcgatca
//
LOCUS       DACEKG010000018        39751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEKG010000018 DACEKG010000000
VERSION     DACEKG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.018"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1082
                     /locus_tag="IW664_000865"
     CDS             33..1082
                     /locus_tag="IW664_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848246.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1079..1960
                     /locus_tag="IW664_000866"
     CDS             1079..1960
                     /locus_tag="IW664_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848247.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            1963..2733
                     /locus_tag="IW664_000867"
     CDS             1963..2733
                     /locus_tag="IW664_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0848248.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3012..3866
                     /gene="aroE"
                     /locus_tag="IW664_000868"
     CDS             3012..3866
                     /gene="aroE"
                     /locus_tag="IW664_000868"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0848249.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4044..5207
                     /locus_tag="IW664_000869"
     CDS             4044..5207
                     /locus_tag="IW664_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0848250.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5324..5734
                     /locus_tag="IW664_000870"
     CDS             5324..5734
                     /locus_tag="IW664_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0848251.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            5908..8136
                     /gene="topB"
                     /locus_tag="IW664_000871"
     CDS             5908..8136
                     /gene="topB"
                     /locus_tag="IW664_000871"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0848252.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8274..8591
                     /locus_tag="IW664_000872"
     CDS             8274..8591
                     /locus_tag="IW664_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848253.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            8751..8987
                     /locus_tag="IW664_000873"
     CDS             8751..8987
                     /locus_tag="IW664_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0848254.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            8984..9382
                     /locus_tag="IW664_000874"
     CDS             8984..9382
                     /locus_tag="IW664_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0848255.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(10049..10993)
                     /locus_tag="IW664_000875"
     CDS             complement(10049..10993)
                     /locus_tag="IW664_000875"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0848256.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(11289..12233)
                     /locus_tag="IW664_000876"
     CDS             complement(11289..12233)
                     /locus_tag="IW664_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848257.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            12454..13668
                     /locus_tag="IW664_000877"
     CDS             12454..13668
                     /locus_tag="IW664_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0848258.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            13690..15705
                     /locus_tag="IW664_000878"
     CDS             13690..15705
                     /locus_tag="IW664_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0848259.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(15788..17161)
                     /locus_tag="IW664_000879"
     CDS             complement(15788..17161)
                     /locus_tag="IW664_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0848260.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(17187..17471)
                     /locus_tag="IW664_000880"
     CDS             complement(17187..17471)
                     /locus_tag="IW664_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0848261.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(17473..19488)
                     /locus_tag="IW664_000881"
     CDS             complement(17473..19488)
                     /locus_tag="IW664_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0848262.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(19667..19972)
                     /locus_tag="IW664_000882"
     CDS             complement(19667..19972)
                     /locus_tag="IW664_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0848263.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(20061..21092)
                     /locus_tag="IW664_000883"
     CDS             complement(20061..21092)
                     /locus_tag="IW664_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0848264.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            21299..22174
                     /locus_tag="IW664_000884"
     CDS             21299..22174
                     /locus_tag="IW664_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0848265.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(22326..23168)
                     /locus_tag="IW664_000885"
     CDS             complement(22326..23168)
                     /locus_tag="IW664_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0848266.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(23260..25137)
                     /locus_tag="IW664_000886"
     CDS             complement(23260..25137)
                     /locus_tag="IW664_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0848267.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(25134..26555)
                     /locus_tag="IW664_000887"
     CDS             complement(25134..26555)
                     /locus_tag="IW664_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0848268.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(26558..29947)
                     /locus_tag="IW664_000888"
     CDS             complement(26558..29947)
                     /locus_tag="IW664_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0848269.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            30204..31595
                     /locus_tag="IW664_000889"
     CDS             30204..31595
                     /locus_tag="IW664_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0848270.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(31668..32315)
                     /locus_tag="IW664_000890"
     CDS             complement(31668..32315)
                     /locus_tag="IW664_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0848271.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(32323..33009)
                     /locus_tag="IW664_000891"
     CDS             complement(32323..33009)
                     /locus_tag="IW664_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848272.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(33015..34088)
                     /locus_tag="IW664_000892"
     CDS             complement(33015..34088)
                     /locus_tag="IW664_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0848273.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            34310..34984
                     /locus_tag="IW664_000893"
     CDS             34310..34984
                     /locus_tag="IW664_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0848274.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            34989..36329
                     /locus_tag="IW664_000894"
     CDS             34989..36329
                     /locus_tag="IW664_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0848275.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(36478..37464)
                     /locus_tag="IW664_000895"
     CDS             complement(36478..37464)
                     /locus_tag="IW664_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0848276.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            38188..39219
                     /locus_tag="IW664_000896"
     CDS             38188..39219
                     /locus_tag="IW664_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0848277.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            39328..>39751
                     /locus_tag="IW664_000897"
     CDS             39328..>39751
                     /locus_tag="IW664_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0848278.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        12299 a         7252 c         7446 g        12754 t
ORIGIN      
        1 aataaaaaga ttgactttgc aggaggaaaa aaatgaaaaa caaacgatta ataacagttg
       61 tagtaattat tttagtttat ttagcaggtg catttattta tggaaaagcc tcaggcgaaa
      121 aagaaagtac ttcgacgaac cagacaaaaa aaacaacagt tgttggggta ttgcaatacg
      181 tgagccatcc ggcattagat gagatttatc gaggaatcaa agatggtctg aaagaagaag
      241 gattaaaaga aggagaaaat cttgagatca aatttcaaaa tggacaagcc gatcagagca
      301 agctagccac aatgagtcaa cagctagtac aatctgatcc agatgccttg attggaatcg
      361 caacacctgc cgctcaatca ttagcaaaca cgacttcatc gatcccagtt gtgttaggtg
      421 ctgtaacgga tcctgtgggg gctggattag tcgcttcttt agagaaacct ggtggtaatg
      481 taacaggtgt ttctgatcag ccgcctgtta cttcacaaat caagcttgct tctgatttgt
      541 tgccaaatgc aaaaaaagtc ggaatcttgt atgcttcttc agaggataat tcaaaatacc
      601 aagtagcaca agcagaagaa gctgcaaaaa aagaagggtt agaatcagta aaatatgcgg
      661 tgccttccac taatgagatt gcacaaacgg ttcaagtgat gagccgacaa gtcgatttta
      721 tctatgttcc attagataat accattgcca atgcgatgca aacagtggta aaagaagcaa
      781 acaaagcaaa tatccccgtc attccatctg tggatacaat ggtggaacaa ggtggactag
      841 caacaatcgg gatcaatcaa tatcaattag ggcttcaaag cggtaagatg gcggcagaac
      901 ttgcgagtgg aaaagaaaaa ccggaaacga cgcctgttta tacatttgat caaggggata
      961 cagtgatcaa tcaatctcaa gctgatcgct taggaatcat tgttccccaa tcgatgaagg
     1021 aaaaagccaa aatcattaca gatgactcgc agcaagaaac atctaaggag gatgacaaat
     1081 gatcgtatca gctatttcac aaggcatgct ttgggcaata ttaggattag ggattttcat
     1141 gacttatcgg atcttagact ttcctgatat gacgacagaa gggtccttcc cattaggtgc
     1201 tgctgtctgt gtgacagcta ttactcatgg ggtagcgccg attgtagcta cattattagg
     1261 cgtaggtgct ggcatgatag caggactcgt tacggggtta ctttatacaa aaggaaagat
     1321 accggttatt ttagcaggga ttttagtgat gtcaggattg aactcagtta ttttgtttgt
     1381 gatgcgaaca ccaaatcttt ctttattgaa tcatccgatt ctacaagaca gcttccatcg
     1441 ctttgggtta cctgattact ttgacacagt ccttcttggc ttcatctctt tgggcatctt
     1501 gattgctgta ctgctattct tcttcaatac agatttgggt caaggctata ttgcgacagg
     1561 agacaacgaa acgatggctc ggtcgttagg gataaaaacc gatcgtatga aaattttagg
     1621 tttaactttg tcgaacggtg tcattgcatt atccggtgcg ctgattgccc agaacgacgg
     1681 atatgcagat gtaaataaag ggattggcgt aatcgtcatc ggtcttgctt ccatcatcat
     1741 cggtgaagtg atcttccaag aattgacgct agcagaaaga ttgattgcta ttgttgtcgg
     1801 cagtatcatt tatcagctat tgattttagt agtaatcaaa ctagggtttg acacgactta
     1861 tctgaagttg ttctccgcta tcatcttagc tatttgtctg atgatcccgc aattgaagaa
     1921 agcactgaac ttaaaaacgg gttttgagaa ggaggcatag taatgactga tatattgaca
     1981 atcaaaaatg gcagaaaagt cgtaaacaat ggaatgaatg aagagaaagt actattggat
     2041 gaaattaacc ttacaatcag agaaggggac ttcatcactg tccttggcgg gaatggtgct
     2101 ggaaaaagca cactgttcaa tacgatcgct ggtacgcttc aactcagtca aggatcgatc
     2161 tcttttaaag atcagctgat cacaaaagat tcagaagaaa aaagagcacg attcctttct
     2221 cgagttttcc aagaccccaa gatgggaacg gctcctcgga tgacagtagc agaaaatttg
     2281 ctacttgcac aaaaaagagg tcaaaaacgg aaattccgtt tacgtaactt gaaaaatcaa
     2341 aaagaagatt ttttcagact atgtcaagaa gtcggtaatg gcctagagca acacttggat
     2401 actccagcgg gtgaattatc tggtggtcaa cgccaagcat taagcttgct gatggcgacg
     2461 atcacagctc ctgagttact attattagat gaacatactg cagctttaga tcctaaaaca
     2521 gctaaagcat taatgcgatt aacaaatcag cggatcaatg aacaaaaatt gacttgtctc
     2581 atgatcacgc atcgaatgga agacgccttg aaatacggaa atcgtctgat cgtactccaa
     2641 aaagggaaaa tcgttcaaga tatttctgaa aaagaaaagc aacatttgac aatgggcgac
     2701 cttttgcagt tctttgaaga agtagtggat tgatttcgac agaaaaattc ctttcagtag
     2761 aaggacttga aaactcattg acatcttcct ttgattcatc ctatacttaa aacaataaac
     2821 aattaaacat atttgggtgt tttgtaggat cacgtagtca atgattttaa caattaaata
     2881 gaatattctt tgctctgaca agtaaacgat gagaaaagaa tttgctactt tgccacctag
     2941 acgtttcgta ctttttcgta tcaaacgcta agtggctttt tttattgaag taagaacaaa
     3001 ggaggaataa gatgatttct ggacaaacca aattagctgg gctctttgct gatccggctt
     3061 ctcacagttt atccccattg atgcacaata ctgcttttga agcaaacaac atcgatgcgg
     3121 tgtatttagc tttccaagta aatccaatga atttgaagca agcagtcgaa agtattcgaa
     3181 cttttgatat gttaggtgta aatctttcga tgccgaataa aacagcggtt attccttatc
     3241 tggatgaggt aagccaggaa gcgcaattga tcggagcagt gaatacgatc gtgcacgaaa
     3301 agggtcgttt gattggctat aatactgatg gtgcgggctt catgcaatcc gtgcaagaag
     3361 ctgggatttc gatcaaaaag aaaaaaatga ctctactggg agctggaggt gcagcaaagg
     3421 cagtcactgt tcaagcagcg ttagacggcg ctgaggaaat cgtagtttat aaacgaaaca
     3481 accaaacatt cgctcaagta aaagaaacat tcgaacaact cagctatcga acttcttgta
     3541 agataaaagt tcgtgacttt gccgatacgc atcaattaag aaaagacttg aaagaaagct
     3601 gtcttctagt caatgcaaca gatatcggga tgggaaagaa aatcaatcag tcaccgctct
     3661 tagatataag catgatgcat gagaatctag cggtttttga tttgatttat gcacctagag
     3721 aaacacgttt attaaaagaa gcaaaaaaag taggagcaaa aggatacaat ggacttggca
     3781 tgttgatcta ccaaggagca atcgcgtttg aattatggac aaaggaaaag atgccgatcc
     3841 atgtgattga aaagctgttt gattaaaaaa ggataataga taaagatctt ttattctcgc
     3901 ataaacggtc tcaagaaaag taatccttta tgtatcgaag catttatttc gttatttttt
     3961 attaaattat caaaaaatga taggcttttg tcgattggtt atcgtatcct ttatttatca
     4021 gtaagaaacg aggaggaacc acaatgagtc ggatggatcg ctataaggac atccataaga
     4081 aggcgaaacc tctgaagcgg gatacgggga gtatttttgc tagaagagaa cgaaaacgaa
     4141 cgcgtatgga aaatgaggaa gatgctaccc gtatttatga aagagagcag cattcatttg
     4201 aacatttgaa ccaatacgag caacagatat acagagatgc accaagagca gaagaacaac
     4261 aaaaaaataa aaaatttttc aatcggcaaa agaagaacaa accgttaaaa cctcgaagaa
     4321 agcgatcgtg gaaaaaaatc attctatcga ttttgcttgt tttgatcctt tattcaggta
     4381 tctcattttt tattggtaaa tatgtagctg aacatgacac aagcttagag gcagcgactt
     4441 cagaaacctt tcatgggcaa aaagcgacga atggtgcgca taatattttg atccttggaa
     4501 gtgatacacg cggagaagat gccgggcgag ccgatacgat catggtctta cagttggatg
     4561 gtccttccca taagccgaaa ctgatttctt ttatgcgtga cagcttcgtt acgatcccag
     4621 gggtaggaca aaataagatc aattcagctt atgcatacgg aggagccgat ctagtgcgtc
     4681 aaactttagt agaaaatttt ggaatcgatt gtcaatacta tgcgaaagtc gatttcaaat
     4741 cgtttgaaaa agtgatcgat gccttattca tgaacggagt gaagatcgat gcagaaaaag
     4801 atctgaatct ggatggtgta gatattaaaa aagggggcca gaagatggat ggacatgttc
     4861 ttttgcagta cgcacgtttc cgaatggatg aacaaggaga ctttggccgt gtcagacgcc
     4921 aacagcaagt aatgaacgct attttcagcc aattgaagaa tccattgaat ctgatccgtg
     4981 caccttatgc agctggtaaa gctttaggat atacatccac agatgtcact gcttggttct
     5041 tgacgaaaaa cctcttttcg attgcacgag gagctggtgg cattgatcga ataagtgtgc
     5101 cagtagacgg ttcgtggaat tatgggcaaa gtgattatgc tggcagtatt cttgaaattg
     5161 ataatcaaat gaatcaatct gctattacag attttttgaa taaatgatca taaaaaagat
     5221 caagaagaag ctgcgcaagg cttttgtcat gaatcaatca aacgattcac tcgtaaaaaa
     5281 cgctttctta tggtagattt aaccaagaga ggagctgttt acattggata tctatctgaa
     5341 cttcaaaaat gagtcgcgtg aagccatcgc tttttatgaa aaagtttttc agactgaagc
     5401 atcaggtatc accacatacg gtgaaatgcc tggaatagaa caaatgaact tgccagaaga
     5461 agcaaaaaat ctaatcacga atgcacggct gaacatttat gatacattag ttatgtttgc
     5521 agatacgcca gaaggaattg gtcctgaatt agttgtggga aataatgttt ctttagtcat
     5581 ccagacaaga aatgaagaag aaacgaagcg cctatttgaa agcttagctg agggtggtaa
     5641 gattgagatg ccactggatg ctgttccgtg ggctcagttg tatgggatgg taactgatcg
     5701 atttggcatc aactggcaga ttaaccaaga gtgacagcta aagatgaata ttttctggaa
     5761 acatcaagca gcaaacggat catcgtttgt ttgcttgatg tttttgtgtc tatttcgact
     5821 atacggtatc ttttttttat tgccagatgc gaaaatatgt tccataatag agaagtccgc
     5881 aaaaaattaa gtaacggagg tcagaagatg aagacggtga tactagctga aaaaccttca
     5941 caagcaaaag cctatgccga aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa
     6001 atcagcgatc cattgtttgc tggtgaagta accatcactt atggttttgg acatttggta
     6061 gatatggttg ctccaggagc ttatgaagag cgttgggcaa aatggtcttt agaaaatttg
     6121 cctattttcc cagaaacctt ccgttacgaa gttccaatgg acaaacgtgc acaattttcg
     6181 atcgtaaaac gagaattgca gtcagccgat accatcatta ttgcaacaga tggtgatcga
     6241 gaaggtgaag ccatcgcgtg gtcgattatt cgacaagcaa acgcattttc taaggaaaag
     6301 aactatttac gtttatggat caattctctt gaaaaagaag caatctatga aggttttaag
     6361 aatctccaac ctggaaagaa ctattttcca aaatataaag aagcgcaagc aaggcagaac
     6421 gctgattggc tgattggaat gaatggaagt ccattatata gcttgctact tcaacgaaaa
     6481 gggattgatg gcagtttttc tcttggtcgt gtacaaacac cgacactcta tatgatttat
     6541 cagctgcaag aagaaatcaa acacttcaaa aaagaaacgt actttgaagg agaaggttta
     6601 attacgacaa acaaagggca gttttctgga aaaatcgtac caaaaaaagc atttaaaacc
     6661 caagaagagt taacgaagga aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga
     6721 cggattcttg aagttacgaa aaaggaaaag aggttgcata gtccacgttt attttcgcta
     6781 tctagtttgc agacaaagat gaatcagcta atgaaagcaa gtgccaaagc tacacttgat
     6841 gctgctcaag gattgtacga aagcaagttt ctaagctatc caagaacgga ttccttttac
     6901 attacagaaa atgagcatca gtatctggtt aaacatctgc aaaattataa aaaattttta
     6961 ggaatagaag aagtacagac aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa
     7021 aaagttcaag aacatcatgc aattattccg acaaagacga ttccgactcc tgcacgcttt
     7081 gcgaagcttt ccaaattaca gcaagcaatc tatttgcaag ttttgcgcac tactgtggct
     7141 atgtttgctg cagattatat ctacgaagag acgaccgtaa tgacgggcgt acagcaatta
     7201 aagctaaaaa gcatcggaaa gatcccatta cagcaaggtt ggcaggaaat cttacgcatc
     7261 aagtcaaaaa agaaagaagc tcagactctt cctcctatcg tagaaaatga agaagcagaa
     7321 gtagaactga aaagtatgga gaaagaaaca caacctccaa aaccctatac cgaaggaacg
     7381 ttgatcacag caatgaaaac tgcgggaaaa acgctggata atgatgaagc gcaagaaatt
     7441 ctcaaagaag tagaaggaat agggacagaa gctacacgag ccaatatcat tgaaaatctt
     7501 aagcagaagc attacatcga agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc
     7561 actctttgta aggcagtagc caatgaacca ttactgacga gcgcagaaat gactgcgcgc
     7621 tgggaagggt acttgagaaa aattggtaaa caagagggga caccatctgt ttttttagag
     7681 aatatcaaaa aattcatcct acatttattg gcagaagttc ctaatcaaat cgacaaggta
     7741 gacttttcag atgagattag cgggacacgt cagataaaag cgatggagaa gaaaaacgat
     7801 caattaggaa gatgtccaaa atgtaaaaag ggaatagtca tgctttatcc taaaatcgct
     7861 acatgcttga atccggaatg cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg
     7921 actaaaacga atctcagaga tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt
     7981 tttactggta aaaaagggaa attcgatgca gttttagtat taaaagaaga tatgacaatc
     8041 ggtttttctt ttccatggat cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa
     8101 acatcaagaa aaaataatga aaacgaagta gagtaaatga aaacaatgaa atagcggaat
     8161 atttattccg aaaagtgaga atataaatac caataaaact aaaaaatatc agtgaatcaa
     8221 gcaaaaacgt tggggtaggc tgattttata tagatagcaa ggagtttttt tatttgaaaa
     8281 aagtgcattc atttttaaat tatcttatgg gagcaatcac gattgtggct gtgtaccaag
     8341 catttctaaa gtatcctgat tacgggacga agttgttgtc attgacgcac aatcaaacag
     8401 catattcacc aacgttgatt ttatcgactt tgactgcagg agctaaaagc gctacttatt
     8461 tttttcataa aacgacaagc atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg
     8521 tggtagcggc tatgatcatt atttattttc taccagttat tacacaagta tttcatcaat
     8581 attttggtta agaatacgag agtttgtgac aaaagtcatg tcacaaactt ttttctttgt
     8641 cattttttcg taagaaaaag caaaataaaa gtatatacat tgattaaaag tatatacttg
     8701 gatataatgt acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa
     8761 tgaaaaagta taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag
     8821 taaaagaatt catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg
     8881 gttctgtacg aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga
     8941 tcatgaatca atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac
     9001 taaagaaatt atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat
     9061 cggtgtaaaa gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt
     9121 caatcaagaa ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc
     9181 taaaaaacat ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt
     9241 tcttcaattg aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat
     9301 aatgattacc actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt
     9361 acgacaaacc gcaataaaat gaacgaatta aaaactttat ttctcaatta gatcagcatg
     9421 tcattaagga atatgctgat ttttttatgt ttaatcatac aaaatagttt tttcataaaa
     9481 atatggaaaa tatggattaa ttttgaaata attgatttaa aaggaataca aaagagtgaa
     9541 tagtaggaaa tagtggttta gaactagtgg gctgattttt tatatatttt agtttacata
     9601 atatatatta tacaaagtag atttaaaaaa tgataaacct ctctcgaacg gttttttatc
     9661 aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt
     9721 ttttcttact ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa
     9781 tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa
     9841 ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat
     9901 atgttcatat gtacggatac attttcggac cgattaattt taaccattct gtttatttgc
     9961 ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt
    10021 aggattttta gttcatattc attgtttctt ataatacagt gttcaatact tctcttaaag
    10081 cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat tcaacaactt
    10141 cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct ttttcgtcgt
    10201 attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc aagatagctt
    10261 ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta cgatcaatga
    10321 tctcataagc agcattacgg actttatcag caatcacatc taattcatct tgggcaatac
    10381 gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta tgtgaccatg
    10441 cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg cgtgggtcaa
    10501 cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt gttccagtac
    10561 cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc aaaatatcta
    10621 ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt tcttttacga
    10681 tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga ccaggttttt
    10741 ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca tagccacctg
    10801 accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc aatgcttccc
    10861 cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt ccttggttga
    10921 tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact actttacgac
    10981 ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt catgaaaagt
    11041 atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat tataattttt
    11101 gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta cacctatatc
    11161 ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac
    11221 gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt tagtgaaaga
    11281 ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata cttcttgaat
    11341 aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc ttccaacgaa
    11401 aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag aaccagttct
    11461 tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg attgtagcca
    11521 aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat ataatctaat
    11581 actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg ttgttctaat
    11641 aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg gatcttatga
    11701 ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa acgacttgtt
    11761 tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga tgcatttatc
    11821 aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc tgtagataag
    11881 gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta ttcgattctt
    11941 tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat cctttctcca
    12001 ttaataattt gtaaacatgt accgctatac atataattga tttcaacaaa atcatgtgta
    12061 tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc ctgttgaaat
    12121 agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt taaatcattg
    12181 atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg caaaaaaaat
    12241 gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc tttcgcttat
    12301 tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa agactttcta
    12361 tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact tcatactata
    12421 tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa
    12481 ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac gtctgcaatt
    12541 cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt attaacgact
    12601 gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc taggaaaatc
    12661 ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg tatcctcccc
    12721 ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg tattggcttt
    12781 ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg agatgaacga
    12841 gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt aatgacattt
    12901 gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta cgccatcagc
    12961 ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac tgaacttcaa
    13021 tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat gattagttat
    13081 ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa attagccaca
    13141 gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt aacaatcatg
    13201 caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa agcacatcgc
    13261 tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat tgccactgtg
    13321 ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt ttcgttattc
    13381 attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga gagtttcaat
    13441 acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc
    13501 tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc
    13561 ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca
    13621 gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg
    13681 tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt
    13741 gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca
    13801 gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt
    13861 gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga
    13921 caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc
    13981 tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca
    14041 gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt
    14101 tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca
    14161 gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc
    14221 tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag
    14281 tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga
    14341 aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga
    14401 atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca
    14461 agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt caagttatgt
    14521 agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg
    14581 tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg
    14641 atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat
    14701 tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt
    14761 ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat
    14821 gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg
    14881 gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg
    14941 atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat
    15001 ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa
    15061 gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg
    15121 aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag
    15181 aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa attctatgaa
    15241 caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat
    15301 tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa
    15361 acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca
    15421 tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag
    15481 ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt
    15541 acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt
    15601 acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca
    15661 aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt
    15721 gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga
    15781 agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc
    15841 tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt
    15901 attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag
    15961 aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca
    16021 ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga
    16081 agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag
    16141 tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc
    16201 caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag
    16261 aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca
    16321 ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg
    16381 caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta
    16441 acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc
    16501 gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa
    16561 aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa
    16621 cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta
    16681 atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg
    16741 cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa
    16801 aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca
    16861 tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc
    16921 taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg
    16981 aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag
    17041 ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca
    17101 aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca
    17161 ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct
    17221 tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa
    17281 gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca
    17341 cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt
    17401 gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg acatactgca
    17461 aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga
    17521 tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa
    17581 agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg
    17641 ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc
    17701 gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa
    17761 cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt
    17821 ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc
    17881 cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt
    17941 cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac
    18001 gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat
    18061 tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa
    18121 gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg
    18181 tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga
    18241 attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa
    18301 tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc
    18361 tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc
    18421 agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta
    18481 gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata
    18541 ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa
    18601 cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc
    18661 cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag
    18721 agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg
    18781 tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat
    18841 ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg
    18901 atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt
    18961 ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag
    19021 tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc
    19081 tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc
    19141 atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg
    19201 aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct
    19261 tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca
    19321 gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg
    19381 agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag
    19441 actgatttct cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac
    19501 ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta
    19561 cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa
    19621 gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat
    19681 gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga
    19741 tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac
    19801 cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt
    19861 ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag
    19921 gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc
    19981 ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa
    20041 acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct
    20101 tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca
    20161 ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt
    20221 actttttctt catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg
    20281 ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct
    20341 tgtcgatctt ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga
    20401 cgttctttga tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa
    20461 tccagatcga agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg
    20521 atatcatctg gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca
    20581 tataaatagc tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa
    20641 taatcataat gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt
    20701 tggactgtca agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct
    20761 ttttcttttg ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc
    20821 tctactagta cattcttacc atgttcgagt gccatttttc catattcata atgaaattga
    20881 ctaggtgtag tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga
    20941 tcagtatatt cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt
    21001 ttgatattga ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc
    21061 tttccattgc caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt
    21121 cagtatatcc tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca
    21181 gaaaaacact tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc
    21241 aaaaagatag tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat
    21301 gcttattgaa gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt
    21361 cgattacatc cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa
    21421 agctacgtat acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg
    21481 atttaaagaa ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa
    21541 tcaaacgatt gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa
    21601 gaaaatggcg gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga
    21661 agatttagat caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa
    21721 aggtgattct caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt
    21781 tttgatcatg gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga
    21841 ggattgcgcc atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac
    21901 ctatttgaag catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat
    21961 ggccaaacag gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt
    22021 agcgactttc tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat
    22081 atattctcaa aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca
    22141 aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga
    22201 aagatagtgt ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt
    22261 ttcaaacctc agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg
    22321 aatagctaac ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg
    22381 aaaatcagta tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact
    22441 ttctgtactt ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt
    22501 aagcgatgac tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct
    22561 ttttcgattc tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga
    22621 taagcgagtg tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt
    22681 aagtctgtga acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta
    22741 ttttctcccc ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc
    22801 acagtattcg tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata
    22861 ccgatcgtat gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta
    22921 aagggatctg cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg
    22981 ttttcttgga ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga
    23041 gagatgatct gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt
    23101 atccctattc ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt
    23161 ggtttcattt gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg
    23221 cgtaatccgc tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg
    23281 tctagcaaag tacagtccag caatcagtag aaggactact cctgcaccga tatacacata
    23341 gattccctta ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg
    23401 agaaatcaac tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt
    23461 tgttaatgga acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt
    23521 tagacctgtc acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt
    23581 cgccgatacc cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc
    23641 actatcttga tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc
    23701 accatctact ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg
    23761 atcatttgta tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc
    23821 tttttcgttc aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg
    23881 aatataattc ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt
    23941 tactgggtcg atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc
    24001 attatcaaat gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt
    24061 ttgcgttcct tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat
    24121 tttttcaccg attgcgacat cttttccatt caattgaggt gttgttttat caactttaga
    24181 cgtatcattt ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg
    24241 cgtgattgtt tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt
    24301 atattttcca acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc
    24361 aaagtcattg ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg
    24421 atcagttgtc caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg
    24481 tgttccttct tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt
    24541 accgattttt gtaactttca acgttccatc attaccgact gtatttttag ggtagagatg
    24601 gatagtatct agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc
    24661 atatacaggg aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc
    24721 tttgatcgtg tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt
    24781 tccatcagca tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc
    24841 ttgttttgct gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac
    24901 gttataaact gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc
    24961 tttcccgctg ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat
    25021 gacttgagct gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa
    25081 tggtaaaata aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt
    25141 ttcactcctg ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca
    25201 acaacgccta aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt
    25261 tcttttttat tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt
    25321 gaaatggtaa acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct
    25381 tttaatcgat aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa
    25441 atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg
    25501 aggtaatttc ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt
    25561 ttatttgtct cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta
    25621 ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct
    25681 gtactgctta gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac
    25741 gaagggatcg aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct
    25801 gtcagctttt ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca
    25861 tccgcactat ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta
    25921 tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc
    25981 ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt
    26041 gaaagttttt gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt
    26101 tctttgacga cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct
    26161 tgatctttag cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt
    26221 gttgcttctg cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct
    26281 tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact
    26341 tggaatgtga caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca
    26401 tttggatgat ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga
    26461 accatttctg tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag
    26521 aagagacaca tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt
    26581 tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta
    26641 aacggccaat tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag
    26701 aaatctggtt gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt
    26761 gaattgaccc atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt
    26821 ctggtgtaaa agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg
    26881 tatctgtttc ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga
    26941 attttgctcc ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg
    27001 cttttagtcg attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc
    27061 ccgtgtaaat gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat
    27121 catatccggc aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa
    27181 aactggcaag acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg
    27241 cttctttttt ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt
    27301 gcatcgcata cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact
    27361 ggatcgtatc atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag
    27421 cgatcttgat ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt
    27481 ctgtcagcgt ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc
    27541 cgtcgccatg atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc
    27601 caataagatc cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga
    27661 attgtttagt atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag
    27721 gtaattcttt gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg
    27781 ataatatctc ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt
    27841 cccacgtatt tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag
    27901 ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag
    27961 ctagattgtt gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt
    28021 tagcagatgg tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg
    28081 tcctgccgtt catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc
    28141 tcacttggta atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga
    28201 tggtatttcc ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct
    28261 tgactgaaag tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc
    28321 cattgcttac agttgctgag atctgtacag cttttttggc taggtattca gagatatctg
    28381 ttgcatgatc agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc
    28441 gcatacgaga ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga
    28501 tgccaagacc atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg
    28561 cgataaacgt actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc
    28621 ttgagattag agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat
    28681 aattgctgct tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat
    28741 ccgtcagcaa aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat
    28801 ctcttagtgc tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta
    28861 cttgattttt cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc
    28921 cttcgcttga gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta
    28981 aagtatcgac aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca
    29041 tacttcctga ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat
    29101 ttccacgagc attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact
    29161 ttcgtatcgc atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc
    29221 gatcattagg atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat
    29281 tgccttgatg atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag
    29341 tgccctgatc atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag
    29401 ctggcaagga taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg
    29461 attcttcttc tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg
    29521 tctcatcaat aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt
    29581 aaagtgctgg atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt
    29641 ccaacgtaat tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca
    29701 catcacttaa gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt
    29761 tattcgcatt tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag
    29821 ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca
    29881 caaagtttga taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg
    29941 tcgtcatttt tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt
    30001 aaaataaaca ggcgttacat aattttattt attttaatat gataattatt atacgaaggg
    30061 cgctaaaggg aatcacacca attttcatac caattggttt catttatgaa gttgattcct
    30121 tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt
    30181 tgttggaacg aaactataga aagatggaaa aaatcattcg aattctgtcc atttctgaca
    30241 agtggatgac taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa
    30301 ggtatatcga agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa
    30361 aattggggta tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa
    30421 tgtcgcaaag ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa
    30481 cgattgagta ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga
    30541 aacaatgcaa tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc
    30601 agttaactag ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa
    30661 gttactacgg aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta
    30721 tggtcaagaa taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt
    30781 tctttattat gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat
    30841 ggaaagctgg agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct
    30901 tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg
    30961 tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac
    31021 gaaaaaatat gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat
    31081 atgttgttca attattaacg caaatttatt ttttctcccg tattttccca tacgatacga
    31141 ctcatttgac gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc
    31201 tatatgctgg atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga
    31261 tttattattt atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt
    31321 atacttctga taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata
    31381 ttgaagaata tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta
    31441 ttactgaaga tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc
    31501 aacccatttt gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa
    31561 atgaagaaga actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa
    31621 accaccctga taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct
    31681 tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta
    31741 tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta
    31801 actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt
    31861 tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc
    31921 tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac
    31981 tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag
    32041 atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa
    32101 tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag
    32161 actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc
    32221 tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta
    32281 tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc
    32341 ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg
    32401 gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa
    32461 tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat
    32521 caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga
    32581 aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc
    32641 gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt
    32701 taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg
    32761 taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc
    32821 tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc
    32881 agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa
    32941 agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat
    33001 ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt
    33061 atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat
    33121 tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca
    33181 aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat
    33241 ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc
    33301 tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag
    33361 tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat
    33421 gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa
    33481 tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca
    33541 tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa
    33601 gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta
    33661 tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt
    33721 ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa
    33781 actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct
    33841 ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact
    33901 tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta
    33961 aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag
    34021 aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct
    34081 agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa
    34141 tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg
    34201 tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat
    34261 ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact
    34321 attagtagta gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat
    34381 cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt
    34441 ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc
    34501 aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt
    34561 ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta
    34621 tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga
    34681 tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta
    34741 taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca
    34801 aaataaggga atcattttga caaaagagca gatatttgac cggatctggg gatttgattc
    34861 tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag
    34921 tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt
    34981 gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg
    35041 tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc
    35101 aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag
    35161 tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg
    35221 aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca
    35281 ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc
    35341 gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata
    35401 ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag
    35461 cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca
    35521 agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc
    35581 tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt
    35641 ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga
    35701 ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat
    35761 ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt
    35821 tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata
    35881 cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa
    35941 aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc
    36001 atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga
    36061 tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc
    36121 cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat
    36181 ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg
    36241 aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag
    36301 tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa
    36361 atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga
    36421 aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta
    36481 cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc
    36541 tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac
    36601 ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt
    36661 aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt
    36721 gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac
    36781 cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt
    36841 ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt
    36901 cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt
    36961 catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc
    37021 tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg
    37081 tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa
    37141 ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt
    37201 ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc
    37261 ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa
    37321 tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc
    37381 ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat
    37441 gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta
    37501 tattgttact tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg
    37561 gaatttgatt aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat
    37621 tctaataaaa tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca
    37681 ctcgttgtga tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag
    37741 gattaattct caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag
    37801 taccaactat ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat
    37861 tattgaataa atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata
    37921 aaagaaagag cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac
    37981 cacatagcta aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa
    38041 ataactaaaa atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg
    38101 cttcctaaga tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa
    38161 aatgaacact attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa
    38221 aagttaaagc aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat
    38281 cgttcttccc tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact
    38341 gattatttga gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa
    38401 aaacaagatt atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact
    38461 acagcgaacc aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag
    38521 caatattcat taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag
    38581 atttctcttt tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat
    38641 gcagaacttt tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc
    38701 agagaagcta gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt
    38761 ctgtattgcg gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg
    38821 accaagcaga ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca
    38881 aaagaagcga tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa
    38941 aattcttttg tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa
    39001 attttgagca aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat
    39061 cgttattatg cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat
    39121 cgatacctct catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat
    39181 cacgaattag cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt
    39241 tcacaataaa ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat
    39301 tacttaacaa aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac
    39361 ttatttacga attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat
    39421 gagccacttt cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa
    39481 agtgtgttga aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt
    39541 attatttata agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt
    39601 ggtgcagtgc ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc
    39661 gatcgtttag aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa
    39721 atttcaaatg gaacaaaatc aaaacagatt c
//
LOCUS       DACEKG010000019        38388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEKG010000019 DACEKG010000000
VERSION     DACEKG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.019"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            519..1403
                     /locus_tag="IW664_000898"
     CDS             519..1403
                     /locus_tag="IW664_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848279.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1612..2190
                     /gene="pgsA"
                     /locus_tag="IW664_000899"
     CDS             1612..2190
                     /gene="pgsA"
                     /locus_tag="IW664_000899"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0848280.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2430..3671
                     /locus_tag="IW664_000900"
     CDS             2430..3671
                     /locus_tag="IW664_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0848281.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3780..4829
                     /gene="recA"
                     /locus_tag="IW664_000901"
     CDS             3780..4829
                     /gene="recA"
                     /locus_tag="IW664_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0848282.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5240..6796
                     /gene="rny"
                     /locus_tag="IW664_000902"
     CDS             5240..6796
                     /gene="rny"
                     /locus_tag="IW664_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0848283.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6875..7681)
                     /gene="proC"
                     /locus_tag="IW664_000903"
     CDS             complement(6875..7681)
                     /gene="proC"
                     /locus_tag="IW664_000903"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0848284.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7822..10395
                     /gene="mprF"
                     /locus_tag="IW664_000904"
     CDS             7822..10395
                     /gene="mprF"
                     /locus_tag="IW664_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0848285.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10549..11400
                     /locus_tag="IW664_000905"
     CDS             10549..11400
                     /locus_tag="IW664_000905"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0848286.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11645..12583
                     /locus_tag="IW664_000906"
     CDS             11645..12583
                     /locus_tag="IW664_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848287.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12606..13286
                     /locus_tag="IW664_000907"
     CDS             12606..13286
                     /locus_tag="IW664_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848288.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13259..14083
                     /locus_tag="IW664_000908"
     CDS             13259..14083
                     /locus_tag="IW664_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0848289.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14250..15068
                     /gene="purR"
                     /locus_tag="IW664_000909"
     CDS             14250..15068
                     /gene="purR"
                     /locus_tag="IW664_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0848290.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15571..16944
                     /gene="glmU"
                     /locus_tag="IW664_000910"
     CDS             15571..16944
                     /gene="glmU"
                     /locus_tag="IW664_000910"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0848291.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            17038..18009
                     /locus_tag="IW664_000911"
     CDS             17038..18009
                     /locus_tag="IW664_000911"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0848292.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      18015..18117
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18183..18752
                     /gene="thiT"
                     /locus_tag="IW664_000912"
     CDS             18183..18752
                     /gene="thiT"
                     /locus_tag="IW664_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0848293.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18833..19606)
                     /locus_tag="IW664_000913"
     CDS             complement(18833..19606)
                     /locus_tag="IW664_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848294.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19872..21572
                     /locus_tag="IW664_000914"
     CDS             19872..21572
                     /locus_tag="IW664_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0848295.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21542..21760
                     /locus_tag="IW664_000915"
     CDS             21542..21760
                     /locus_tag="IW664_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848296.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21780..22670
                     /locus_tag="IW664_000916"
     CDS             21780..22670
                     /locus_tag="IW664_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0848297.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22688..22972
                     /locus_tag="IW664_000917"
     CDS             22688..22972
                     /locus_tag="IW664_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848298.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23459..24073
                     /gene="gmk"
                     /locus_tag="IW664_000918"
     CDS             23459..24073
                     /gene="gmk"
                     /locus_tag="IW664_000918"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0848299.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            24078..24383
                     /locus_tag="IW664_000919"
     CDS             24078..24383
                     /locus_tag="IW664_000919"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0848300.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24636..27044
                     /gene="priA"
                     /locus_tag="IW664_000920"
     CDS             24636..27044
                     /gene="priA"
                     /locus_tag="IW664_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0848301.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            27059..27550
                     /gene="def"
                     /locus_tag="IW664_000921"
     CDS             27059..27550
                     /gene="def"
                     /locus_tag="IW664_000921"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0848302.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27543..28481
                     /locus_tag="IW664_000922"
     CDS             27543..28481
                     /locus_tag="IW664_000922"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0848303.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28481..29839
                     /gene="rsmB"
                     /locus_tag="IW664_000923"
     CDS             28481..29839
                     /gene="rsmB"
                     /locus_tag="IW664_000923"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0848304.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29852..30592
                     /locus_tag="IW664_000924"
     CDS             29852..30592
                     /locus_tag="IW664_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0848305.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30589..32658
                     /gene="pknB"
                     /locus_tag="IW664_000925"
     CDS             30589..32658
                     /gene="pknB"
                     /locus_tag="IW664_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0848306.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32823..33722
                     /gene="rsgA"
                     /locus_tag="IW664_000926"
     CDS             32823..33722
                     /gene="rsgA"
                     /locus_tag="IW664_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0848307.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33735..34385
                     /locus_tag="IW664_000927"
     CDS             33735..34385
                     /locus_tag="IW664_000927"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0848308.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34386..35024
                     /locus_tag="IW664_000928"
     CDS             34386..35024
                     /locus_tag="IW664_000928"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0848309.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            35179..36033
                     /gene="mreC"
                     /locus_tag="IW664_000929"
     CDS             35179..36033
                     /gene="mreC"
                     /locus_tag="IW664_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0848310.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            36037..36546
                     /gene="mreD"
                     /locus_tag="IW664_000930"
     CDS             36037..36546
                     /gene="mreD"
                     /locus_tag="IW664_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0848311.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36801..38339
                     /locus_tag="IW664_000931"
     CDS             36801..38339
                     /locus_tag="IW664_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848312.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT        12621 a         6866 c         8352 g        10549 t
ORIGIN      
        1 gcttattgcc gaagaagcta cttctggaac accgtgtacc aagaggttgt gaaaaagctg
       61 ccctgcatca agtaataaga acagccaagt aaaaacagct tcttaatgtt tttaacttgg
      121 ttggacttat taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat
      181 cagcgttttg acctgagtat tagagaaaca tacggaaaaa tatctcctta tattttgccg
      241 gtttttgagc taataccgag gaacagctga ttgaacaccg tttaagaaag aggtcgtgag
      301 cctgccgttt aactccgagt cacaaggaac aagttcaaaa aacagcttct tatatttatt
      361 ggatattgtg acttgtgacc gaggagttgg caggagaaca atgtttattc ggaaaaaagg
      421 agctgtgaca tgacttttgt cacagcaccc aaacataacg tctttcgttg caacagcaga
      481 tgcttcggag aatcaaacag actggagaag atgaacgagt ggcttttgaa accatcggag
      541 aaaaattacg gcaagcacgc ctgaataaaa aaatatcttt agatgaatta cagcagatta
      601 caaaaattca aaaacgttat ttggaagcaa tcgacaatga tgattttgac caattgcctg
      661 gtaaatttta cgtgcgggca tttattcggc aatacgctga agctgtagga gaagatggag
      721 accatttagt cgatgttttt gatgggaaaa gcaaattgac aggatcggtc cctgaacgtc
      781 cgcagcctga aacagtaagt acttctagaa aagcaatgca tcaggaagaa tcgcatccaa
      841 gtcgcttttg gacaagtctt cctgtcattt tattaggatt ggttgctttg gcgattgtcg
      901 ttgtcgtggg ctatatgact tggcaagatc gtcaagctga tccaataatc ggagacacag
      961 cttcttcgct taaagtagaa ggatcagtcg aggagtcctc ttcctcagag caaagcacgc
     1021 agaccaaaga gtcttctaca gtagaatcaa caacaaaatc aagtgaagaa aagaaaatgg
     1081 caatagctac aggagaagat tctggcagtg ccatcgcagt aaatatcaca gacgctacca
     1141 agcctgtaac ccttgaattt actgctgtca atcgtgtatg gttaggtgtg ctagtagaca
     1201 atgcctatgt ttatcaaggg acactggcag caaatgaaac acagagtacc gtgttaccgg
     1261 aaacagcaac aaatgcgacc atcacgattg gtgcagcaag caatgcaacg atcaaagcga
     1321 atggagaatc cgtaccagtt aatccagggg aaaacaatca gagtccaaaa aatgtgaacc
     1381 ttactcttca atacgcagaa tgatcgtata aattagttta cgcatctgtt tacttgtagg
     1441 tataaaaaag aaggatcgtg acaaatctgt gtcgcggtcc ttcttcgtta attcaaaaag
     1501 atcaatttca ataaaaatac tcctttgaat aaattcattt ggaaaagtaa ataatatcgg
     1561 tggaaacttc gctgatagtc acgtagaata gggaagaaag gagtcgatag tgtgaattta
     1621 ccaaataaat taactgtact tagaatttgt atgatcccga ttttcatttt ggttgtaggt
     1681 ttacctttag attggggaca atttaacgta ggagacacca cactggctgt cacacagcta
     1741 gtaggtgcag ttatttttgc tttagctagt atcacagact ggttagatgg aaaaattgcc
     1801 cgtgcgcgag gccttgtcac taattttggc aagtttgcgg acccgttggc agataaaatg
     1861 ctagttatga ctgcttttat catgttggtt gaccaaggaa aggcccctgg ttgggtagta
     1921 gcgatcattg tctgtcgtga attagccgtc acaggcttgc gtttactact tgtcgaacat
     1981 ggagaagtca tggctgcagc ttggccggga aaagtcaaga cggctacaca aatggttgct
     2041 attatcttgc tgtttatcaa taatatccca ttcagcctga tcggtattcc agtagatcaa
     2101 atcatgcttt atgcttgtct gatctttact atctattcag gtgtggatta cttcatcaaa
     2161 aacagcggtg tgttcaaagg atcaatgtaa gtatcgttta gtggagaggt tgtgagcctg
     2221 ccgtctaact ctgagtcaca agggataatt tcaaaaaaca gcttttcatg ttttatgtat
     2281 attgtgactt atgaccgagg ttttggcagg agaacactgt ttatttggaa caaggagctg
     2341 tgacaagatt tttgtcacag ctccttgccc tctttttaaa taaaaaaata taaaaataca
     2401 gcgtatacta tataagggag cgggaagtaa tgaaagcaga aataatagct gtcggaacag
     2461 aacttttact aggacaagtc gtgaatacga atgccacttt cttgtcagaa cagctagctg
     2521 atctaggtat cgaagtttat tatcaaacag ttgtaggaga taatcagcag cgtttagaag
     2581 agttgattgc tctagctgaa acaagaagtg aactcatact cttatgtggc ggattaggac
     2641 cgacagaaga tgacctgaca aaagaagcaa ctgcggctca cctcggtaag tctctcatac
     2701 aaaatacgga aggatacaaa aaattattgg catattttga aacaacccat cgaaaaatga
     2761 ccaaaaacaa tctgcaacag tcccagatta tcgaaggcgg ggttcctcta ccaaatcgta
     2821 cagggcttgc tttagggact ttttatcaaa cagatacaca tgcgtatatc cttcttcccg
     2881 gtccgcctaa tgagttgaaa ccaatgtttg tggaacaagt acgtccttta ctagaggaac
     2941 gttttccttc tgaagaaaaa ctaatttcta aagttttgcg cttttatggg attggtgaat
     3001 cgcgactggt cactgagcta aaagatctga ttgagacaca gatcaatcca acgatcgcac
     3061 catatgccaa accaaatgaa gtgacattac gactgacagt gaaaacaaat gatgttcagg
     3121 cagggaatca agctctttta gcattagaag aaaaaatcca agaacgagta ggggaatatt
     3181 tttatggata cggcgatgat aattcgctag caaaagtcgt agtggaacta ctgaaagaaa
     3241 acaagcagac tgtaacagca gcggaaagtt tgacagccgg cgccttccaa gccgcattag
     3301 gcgacattgc tggtgtatct gaagtatttc caggcggatt cgttacctac tcgctccaaa
     3361 caaaagctgg ttttctggag attgatccgg agctattagc agaatatgga acagtcagca
     3421 aagaatgtgt ggagcagatg gccattcaag cacgtgagat agcaagaaca gattacagtg
     3481 tagctttcac cggagtggct ggaccggaca cgttagaagg tcaatctgca ggaacagtat
     3541 ggatcgctat ttcatctaaa gacggcgtgg tttcggaaaa atatcacttt gccagagatc
     3601 gtgctgctat cagatataat gcggttatgc aaggatttga tcttttgaga aaacagcttt
     3661 taagaaaatg ataaatgcga atatttgttc ggttttttct tgcttttttt cactaaaaaa
     3721 gatatgataa ggataatgaa atgttgctct gatttgtaaa tataaggagg attatcgtat
     3781 tggcagatga tcgtaaagca gccttagatg ctgcactgaa aaaaattgaa aagagctatg
     3841 gtaaaggctc tattatgaaa ttgggcgaaa aaatcgacca acaaatctct acaattccaa
     3901 gtggctctct tgcgttagat gtcgctttag gtgtcggcgg ttacccccgt ggacgtatca
     3961 tcgaagtata tggacctgaa agttcaggta aaacaacagt tgcactacac gctattgcag
     4021 aagtacaaaa aaatggcgga acggccgctt tcattgatgc tgagcatgcg ttagatccgc
     4081 aatatgcaca aaaattaggt gtgaatatcg atgaactact tctttcacag cctgacacag
     4141 gagaacaagg tctagagatc gctgatgctt tagtatcaag tggggctgta gatatcgtag
     4201 tagtcgattc agttgctgct ttagttccac gagcagaaat cgacggcgaa atgggtgact
     4261 cacatgtcgg gttacaagca cgtttgatgt ctcaagcatt gcgtaaactc tctggttcga
     4321 tcaacaaaac aaaaacaatc gctattttca tcaaccaaat ccgtgaaaaa gtaggcgtga
     4381 tgtttgggaa tccagaaatc actcctggag gacgcgcatt gaaattctac gcaacgatcc
     4441 gtttggaagt acgtcgtgca gaacaattga aacaaggtac agatattgtt ggtaaccgta
     4501 caaagatcaa agtcgtaaaa aacaaagtag cacctccttt caaaatcgct gaagtagatg
     4561 tcatgtatgg tttagggatc tcacaagaag gagaacttct agacatggca gtagaaaaag
     4621 acattgttga taaaagcggt gcttggtatt cttataaaga agatcggatt ggtcaaggac
     4681 gggaaaacgc taaaatctat atggctaatc atcctgaaat gatggcagaa gtttctgctc
     4741 tagttagagc agcttatggt attggtgaag aagtcgctgt cccagaagat gaaaaaggac
     4801 aagaagaatt gcctttagta gaagaataaa aattatgata aacaaaaacg gctggtctga
     4861 agagtaggta ctctccttga cctgccgttt ttttattgaa taaagaaatc gtgagttatt
     4921 taattatacg agtgggtaca taaaataaaa atatctgttc atctacaaag aataatttaa
     4981 tagaaaagtt tctcaattta aaggtttacc tatatgaaac tagtgtcatt tattttaaaa
     5041 accatcgttt aatgaataag tagaaagccg tttctttgtt tatttaaagt tgacactacg
     5101 gggtacaaac attagaataa agttgtgcat gttttttgta cacatatatg cgaagatgca
     5161 tattgacgag tttagaataa tttatcatag aagctgtata gatgataaaa atgaaaaaga
     5221 tagtatggag gtggatttta tggtattcaa cattctcctc gctatcatcg gtttaattgt
     5281 cggtcttggt ttagggttta tggtcgcaaa gtcacgtcac gagaaagaaa tagctggtgc
     5341 acaatcatca gctgctggaa tcatcgatag tgcaaaaaaa gaagcagaaa ctctaaaaaa
     5401 agaagcattg ttagaagcta aggaagaaaa tcagaaatac cgttctgaag ttgaaagtga
     5461 gctgagagaa agtaagttag aattaaaatc tcaagagaat cgtttgattc aacgcgaaca
     5521 aacgctgaat cgtaaagatg actcgttaga aaaacgcgag aactcacttg aaagtaaaga
     5581 agaagcactt agttcaaaac agcaattaat tgaagagcga gaaaaagacg ttgaaaaaat
     5641 gattgaagat caacaaaaag aactagaaaa aattgctggt ttatcaaaag aggacgcacg
     5701 agatgtcatc atgaaatcga ctgaagaaga gctgaatcat gagttaactc taatggtaaa
     5761 agaatctgaa cagcgtgcca aagaagaagc agatcgcaaa gcgaaaaact tattatcatt
     5821 agcgattcaa cgctgcgcag cagatcaagt ttcagaaacg actgtttcag tcgttaccct
     5881 tccgaatgat gaaatgaaag gtcgtatcat tggacgagaa ggtcgtaata ttcgaacgct
     5941 tgagacgctg actggtatcg atttaatcat cgatgataca ccggaagcag ttgttctttc
     6001 cggattcgat ccaatccgaa gagaaatcgc acggatgacc ttggaaaaac tgatacaaga
     6061 tggacgaatc catccagcgc gtatcgaaga aatggtcgag aaatctcgta aagaaatgga
     6121 tgaacgtatt cgcgaatacg gggaagaggc agcgtttgaa gtcggtgctc atacacttca
     6181 tccagatcta atcaagatca tcggacgtct gcgattcaga actagttatg gacaaaacgt
     6241 cttgaagcat tcggtcgagg ttgcaaaatt agctggtatc ttagcagcag agcttggcga
     6301 agatatccaa ttagcaaaac gtgccgggtt gctccatgat attgggaaag cacttgacca
     6361 tgagattgaa ggatctcacg ttgagatcgg tgcggaatta gctgccaagt acaaagaaaa
     6421 cccagtagta atcaacgcga tcgcttctca tcatggagat gtggaagcta cttctgttat
     6481 ctctgtcctt gtagctgcag cagatgcgct ctcagcagca agacctggtg cgcgaagtga
     6541 atcattagaa aactacatcc gtcgtttaag aagtctggaa aatatttcga acagcttcgc
     6601 cggtgtagaa tcaagttacg cagttcaagc tggacgcgaa gttcgggtaa tggtcaagcc
     6661 agaagaaatc tcggatttag acgctgttcg tttagtacgt gatattcgta aacgaatcga
     6721 agaagaactg gattatcctg gacacatcaa agtaatcgtt attcgggaaa ctcgtgcaac
     6781 tgattatgct aaataataaa aaatacaaaa gaagagccgc ggcaaaagcc acagctcttc
     6841 tttttttgta aggtacgaaa aaacattgct atcattattt tttattcaac atctcaagat
     6901 ctttctcgat cgtcgtattg attcctttga tgattgctgg gatgaatcct gcttcttcta
     6961 aggcaactac acctgcaaca gttgtaccgc caggagaaga aacttggtct acaagttccc
     7021 atggattttt atcacttgaa aggaattttt gacaagaacc cataattgct tttgcagcaa
     7081 tctcagtggc tgtctgtttg gacaaaccat tcagtacacc agtccgtccc atcgcatcga
     7141 tcagcatgta aacgaatgcg ggtgagctgc cagcaaggcc aatgaatgta ctgaaatctg
     7201 attctgctaa aggatagacg ctgccgattg cttcaaaaat catcgtagct tgctgaagct
     7261 gttcctccgt ggcagcttca tttttgcaaa tcgcagaaac gcctgctcct acgctgacat
     7321 tgacattcgg catcacacgt acgatggagg cctgaggatc ttcaagcgct gtttctaaat
     7381 ctgttaatga ttttcctgaa gcaatagaaa tgaataagac tttctggcta tattctctta
     7441 gttcttccaa tactttttca atgatttttg gtttcacggc taaaagaacg atatctgatt
     7501 gtgatgcaac ttcctgatta cttttgcatg catggacgcc agtttcagca gcaaattttt
     7561 gtgcattttc aaaatgtcca ctggaaacga acacctcttc tttcgagact gttccttttt
     7621 ttaataaacc atcaataata gcttgtgcca tattccctgt gccgataaat cctactttca
     7681 tgttatcctc tcctttagca ttaggatatc tttctttttc tatcatcgca agcaaagaac
     7741 aggtctaaag tggaaaaatt aaaataaata agataacatt ggggtatgac acaaaagtat
     7801 acaagttagg aatgggaaaa tgtgaaaaat aaacgatact tgatgctgca atggataaag
     7861 gaacatagtc tgattttaaa actgattttt ttcggctccg tgttagtctt tgtggcaaat
     7921 caagtcgcaa atattgctaa tggcatgagt tggcaagata tttggcaaag gatgggacaa
     7981 caaagccgca tgacgatcct gctcatgatt ctggctggac tgctgggtgt cactccgatg
     8041 ttgttgtatg attgggtgac gatcagagtc ttagaaaaac aaggaaaacc aaaaatggaa
     8101 agaaaagagt ttcttcttgc tgcgtgggta acaaacacga tcaacaatct cgccggtttt
     8161 ggtggtgtgg taggagcaag tctgcgggct agtttttatg gaaagcagac aaatagaaaa
     8221 atggttttag ccactgtatc aaaagtcgca ctatttatgc tgactggttt gtctgtatgg
     8281 tcgtttctta catttattga tgtctttttt attcagtcag aaagtatttt tcgaagctat
     8341 tggatctggc ttttcggcgg tagtttttta gcaccagtca ttttcttttt tgcttatcta
     8401 aagagaaaga cattgttctt cgagttttat ccaaaaggct tgcttggtct gctactggca
     8461 tcctttggac aatggacggg agcccttgcg gtatttttga tcatcggcaa gttgatgaga
     8521 atagatattt cgatgctttc gatctatcct atgttcatga ttgctacatt gataggaatg
     8581 ctgacgatgg tcccaggtgg aatggggact tttgacgttt tgatgatctt aggaatgagt
     8641 caattgtctg ttcggcaaga tgtagcagta gtttggctat tatattatcg gttgttttat
     8701 tatgttctac cttttatgac gggacttcta ttgttcatca cacatacagg agtcaaaatc
     8761 aatcgatttt tagataacat tccgcgactg tttttccaaa aggttgcgca tatcgtgctc
     8821 gtaagtgctg tctattttgc ggggatcatg atggtacttc tatcaacaat cactaatctg
     8881 tctgcaatca gtcggctatt tcaagtatta ttgccgttct cattcaattt tttagatcag
     8941 acagtgaatc tattggttgg ctttttatta ctcggccttg ctagagcact ttcagtaaaa
     9001 gtcaaaaagg catttctgcc tacgattgct ttactatctt ttggtatttt aaatacaatc
     9061 acacgtacgt tatcttggca gttgatattg gtatatgtct tgattctgtt agctgtgtgg
     9121 ttatccagaa aagaatttta tcgaacaaaa ttcgtttatt cttggggagc tataatcttt
     9181 gatgctagct tgttcggctt tttgtttatc gtttacgcaa ttgcaggcta tcacagcgga
     9241 cgctttttag tcggtcgaat aacaggaaat cattttattt tgtttccttc agatgatgtg
     9301 tggttctcag ggcttattgg tttgggcatc tcattgctag ctttagtggc gctttatcaa
     9361 tatttagctg aagatactac gaagcttggc agtgagtacg aaaaagaccg ttttgaccgt
     9421 ttattggaaa catacggagg cacagctaca agccatcagc ttcgcttgtc aggttacagt
     9481 tatttttatt accaagaaga cgaccaagat caagtcgttt ttggttatca gatcaaagga
     9541 aacaaatgct ttgttttagg agatccagtt ggcaatccta aaatgatgcg ggcagcaacg
     9601 aatcagttta tgaaacaagc agattgttta ggctatcagc ttgcgtttta taagatatca
     9661 gaaagctacg tagtgatgct tcatgaattg ggttttcact tcacgaaagt aggtgaagca
     9721 gggattgttg atctagcaac agaagacttg tctcggctga gtcagcaact tgaatatcaa
     9781 cgtttgatga aagaaggata ccattttact tggtacgatc aactgccgga agaattacat
     9841 caagctgtaa atgaagtttc acaaaaatgg cttcaaggtg cacgagaaaa atacttctct
     9901 gtgggacgat ttgataaatc atatgtatta tctagcggtg tcggtattgt ccgtaaagat
     9961 caaaaagtcg ttggcttcat caccgctcag ccgatgacga agaagcaggc gggatatgat
    10021 ttgttgcggg tcttacctaa agaatctgag gaattagctg actatctgtt agccaatctg
    10081 tttattgtat acgagaaaat gggctactcg gaagccaatc taggcttagc tccattagct
    10141 aatgttggag aaacagattt ttctttcttt caagaaaaag tcatgcatat cgtatataat
    10201 tatggtaatt ttttctattc cttccagtct gcttatgaaa agaaattaaa atatgtcgat
    10261 cattgggaag gacgttattt tgcttatagg aaaggaagta atttcggatt tgctaccatg
    10321 cagctactca gtctgatagg aaaagaaaaa ggaaatgttc cgagtttggc agaagaagtg
    10381 ctcacagaat tataagaatc actagaactt gtttggatta tgaagagaaa attctgtgaa
    10441 agctgttgaa acgatgacaa aatcaaccac taggcagaca taagctatac ttttattttt
    10501 ccctatgata gaatacttgc gacttagact tttcgaatgg gtgagaggat ggaattgatt
    10561 gaaaaagcgc cagccaagat aaatttagga ctggatgtat tatatagacg gcaagatggg
    10621 tatcatgagt tggaaatggt gatgtcgagc gtagacttgg cagatcatct attttttgaa
    10681 gaaataacag aagacaaaat cattgtagaa acaaataaag catttttacc ggtagatgaa
    10741 cggaacaatg tttatcaagc tgctttattg ctaaaaaata aatacaatat caaaaccggt
    10801 gtcaagatct ttgttaagaa gaatattcca gtagcagctg gattaggcgg aggaagtaca
    10861 gactgtgcag ctgctctaag agggatcaac caattatgga atctgggctg ttcgttagaa
    10921 gaactggcag aaatcggttt ggaagtcggt acagatgttc cttactgtgt ctacggaagt
    10981 acggcatttg tcggtggcaa aggggaagaa atccaagtat taccttctat gccacagtgc
    11041 tgggttgtgt tggtcaaacc aagaatgagt gtctctacac gtacagtgtt tagagaagta
    11101 gatacgcaag ccatccaaca tcaagacatc cacggacttg ctgaagccat ccgagagcaa
    11161 gattatcagc agatgatcaa aaaaatggga aacagtttag aagatataac gggaaagcgg
    11221 catccagtca tccatcaaat caaagagcgt atgatcaagt acggagcaga tgctgcattg
    11281 atgagtggaa gcggaccaac cgtttttgcc ttgtgtcaac attattcaag agcgcagaga
    11341 gtatataatg ggttaaaagg tttttgtgag gaagtttatt tagtacgaac cctgcaataa
    11401 aatataagaa gtaaaatagg tgatgaaaaa ctgaggaatc ctaaaatggt attcttcagt
    11461 tttttattgt agtgaaatgg ctagatatga gcagcaactc atccatttta cgatttaatc
    11521 aagaatatac atgcttaaat cagaaattga tggatttcta ttgactgtgt ttttttattt
    11581 tgatacatta gttcacgtta ataaatcgta aaattccgat ttgttcattg aaaggagcat
    11641 ataaatgaaa aaatatcttt tgacagctac ggtaatgata ggtgctttac tgtttgcagc
    11701 atgtggaaat acgaacaagg aagcagacaa aaaagaggat ttgacgatcg ttacgacttt
    11761 ttatccgatg tatgatttta caaaagagat cgtaggggat gaaggaaacg tgaaactttt
    11821 gatccctgct ggaacggagc cgcatgattt cgaaccaagt gcaaaagaaa gagcagaaat
    11881 ttccgatgca gatgttttcg tgtataatag ttcagacatg gaattttttg tggattcgtt
    11941 aaaggattca gtggatagca aacagacctt gatgatcgaa gcagctaaag gaatcgaccg
    12001 tttagaatcg caagaagcag acgaacatga agaatcagaa gaagggcatg gtcattcaca
    12061 tgaatacgat ccacatgttt ggctcgatcc ggtattagct ataaaagaag tcagaacgat
    12121 tgctgaagaa ttaggcgaaa aatatccaga taaaaaggaa acttttacga agaatgccga
    12181 tgcttatatc aaaaagcttg aagcactgga tcaaaaatac tccgatgagc taaaagatgc
    12241 aacaaaccgc acatttgtca ctcagcatgc tgcttttgct tatttagcga atcaatacga
    12301 cttaaaacaa gtagcgattt cgggcgtttc accagatcag gaacccacac caagtcgctt
    12361 agctgagcta aaagaatttg tgaagaaaaa caacatcaaa gtgatttatt ttgaagaaaa
    12421 tgcctcttcg aaggtagctg agacattgtc gaatgaaaca ggagtgaagc tagaagtgtt
    12481 gaatccttta gaaagcttga ctaatgaaca aatcaaagca ggcgaaaatt atatttctgt
    12541 catggaaaaa aatctgaaag ctttaaaaga aagtatcaat taagttttag aatggaggtt
    12601 gtaaggtgcg ttatatcgaa atatctgatt tgacctttta ctatgatgat gaacctgtac
    12661 tagaagatgt ctcttatcat gtagatgcgg gtgaatttgt cattttaact ggagaaaatg
    12721 gagcagccaa gtcaaccttg gtgaaagcat cgttaggatt actgaaacct tcaaaaggaa
    12781 tgataaagat tgctgagaaa aatcaagaag gaaaaaaatt aagtatcggt tatattcctc
    12841 aacaagttgc atctttcaat gcaggttttc ctagtacggt gatcgaatta gtccgttcag
    12901 gaagatatcc aagaggccgc tggttcaagc gtctctctga aaaagatcat caacatgtac
    12961 agcgtgcttt agaagctgta gggatgtggg atatgcgaaa tcggcgaata ggtgaattgt
    13021 ctggcggaca aaagcagcga atcagtttag cgcgtatttt tgctactgac cctgacttat
    13081 ttgttcttga tgaaccaaca acaggtatgg atgaagaatc cagaaatgaa ttttatcgtc
    13141 ttctccgaca tagtgcgcat gtccatggga aatcagttct aatgatcacc catgatcatg
    13201 aagatatcaa acagtatgcg gacagacaga tccgactggt gagaaaggag gattctcaat
    13261 ggcgttgctt tcatatgaat tcatgagaag agcgtttctt gccgctatct tcattgccgg
    13321 aatcgccccg atgctaggtg tgtttttagt gatcagaaga caatcgctga tggctgatac
    13381 gctttctcat gtctctttag caggggtagc gttaggattc tttttgaacc ttaatcctac
    13441 acttacgacc atgctagtcg ttgtattagc agctgtgata ttggagtatc tgcgaacgat
    13501 ctaccgaacc tattcagaaa tctccattgc cattttaatg gctggtggtt tggcgctagc
    13561 gttagtattg atgaatttga gcggagggaa ttcagcaaca agtatccaat cctatctgtt
    13621 tggttcgatc gtgaccatta catgggagca agtcatcttt ttaggaatct tattcgttgc
    13681 catcggtata ttgttctttt tattcaaacg gccgatgtat gttctgactt ttgatgaaga
    13741 taccgcccac gtggatgggc tacccgttca tttgatgtcg atgatgttca atgtgattac
    13801 tggtgtggct attgctgtga tgatcccgat tgccggtgca cttttgatat ccgcaatcat
    13861 ggttcttccc gcagcaatcg gtatgcgatt aggcaaaagc ttcaatgtcg tgattgctat
    13921 cagtgtttgt gtaggatttc tagggatgct gtcaggacta actagctcgt tttatctaga
    13981 tacacctcca ggagcaacaa tcacattagt ctttattgct ttgttcctga ttgtaaatat
    14041 catcaaacga atattggttg ccttcagaag aaaaaataac taaattataa agaaaagaag
    14101 ccttataaaa acgaaaaatt ttttataggc ttctttttat tttaataaat accgaactat
    14161 tttcttattt tttcataaaa tgctcgaatt tttgaagaaa aacttatata atgtaaaacg
    14221 ataaaatttc ttaaaaggag ttggaatagg tgaaagtacg tcgcagtgaa cgtctagttg
    14281 acatgacaca gtatttattg aatcacccgc atgaattgat ttccttaaca agctttgccg
    14341 aacgttatga atctgctaaa tcttctatca gtgaagattt gggaatcatc aagaaaacat
    14401 ttaaagaacg agggtatgga accttggaaa caatcccggg tgctgcaggt ggtgtattgt
    14461 tcatccctga aatttcatat gaagaggcga aaaaatatat tgaatctcta gcagaacgtc
    14521 tatcagaaca agaccgtctg cttccaggag gatatgttta tctctctgat ttgttaggag
    14581 aacctgaact gctgcgtcaa gttggaagaa tcattgcttc taaatacctt ggtgaaaaaa
    14641 tcgatgctgt aatgactgta gccacaaaag gcgtacctat tgcccaagct gtgtcctatt
    14701 atctgaatgc gccattcgtt attgtacgcc gggactcaaa aataacagaa ggatcaacag
    14761 taagtgtcaa ctatgtgtct ggatcttctg aaagaattga aaagatggaa ctgtctaaga
    14821 gaagtttgaa acgtggttca cgtgtccttg tagtggatga tttcatgaaa ggcggcggaa
    14881 cagtcaacgg aatgaagagc ttgattgaag aattcgaatc agaacttgtt ggcatcactg
    14941 tatttgccga atcaaaattc aacggtcatc gcgccatcga tgattatacg tcactgcttt
    15001 atgtcaaaga tgtagatact caaacgaaaa atattacggt tgtgccagga aattattttt
    15061 cagaataaga ggttgtgaaa aagccgctta gtttcgagta gtaagaaaaa ttttgtgaaa
    15121 atggctttcc acattttttc ataaatttgg cttaataccg agaaactggc ttttgaacac
    15181 cgtttatcta gaggttgtga aatcagcgtc ctgtcctgag tattagctca ataaacggaa
    15241 aaattgctcc tcatattttg ccgtttattg agctaatacc gaaggtcagc tgattgaaaa
    15301 acgtttaatg aagaggttgt aagcctaccg tttaactcta agtcacaaga aacgatttca
    15361 aaaaacagct tttcatgttt tatgagtagt gtgacttgtg accgaggagt tggcaggaga
    15421 gcaccggtta ccaaatagat aggcccgtta tggtctatct atttttttat ccagaaaacg
    15481 ctgggataaa agaaaatcac ttgttttgtt gaaaaaatgg gtatactggg ataaactgaa
    15541 atcagcaaac tatatttagg agtgacgcag ttggacgcac gttatgcaat tattttggcg
    15601 gcagggaaag gtacccgcat gaagtcaaaa ttatataaag ttttacatcc cgtttctggt
    15661 aaacctatgg tagaacatat cattaatcgg gtcagtgaaa cgaaaccaga tgaagtgatt
    15721 acgatcgttg gacacggagc agaacaagtt aaagcacaac taggagaacg cagtaaatat
    15781 gcgttacaag cagaacaatt gggaacagga catgctgttt tgcaagctgc ttctttcttg
    15841 gaaggaaaaa aaggaacaac gttagttatc agtggcgata caccattatt gactactgaa
    15901 acgctgaaca atctttttga atatcaccaa ggaaaaaatg caagtgcaac gatcttgacg
    15961 gcacaagcag aaaacccaac aggatatgga cgaatcatcc gtgaccatat tgggatcgta
    16021 gaaaaaatcg tggaacaaaa agatgcaaca ccagaagaag ctcttgtaca agaaatcaat
    16081 acaggaactt actgttttga caacgaagct ttgttcgatg cgttaagcaa agttggaaca
    16141 aacaatgctc aaggtgaata ctatctaaca gatatcatcg aaatcttaaa agaagaaggc
    16201 catacagttg ctgcttatca aacggatgat tttgaagaat caatgggagt caatgatcgg
    16261 atcgcgttag ctaaagcaaa tgagatcatg agaaagcgaa ttaaccagat gcatatggtc
    16321 aatggcgtaa gctttgtcga ttcagcaacg acatacatcg atgcaggcgt agaaatcggt
    16381 ccagacacat tgatcgaagc aggtgtacaa atccaaggaa atacagtcat tggttctgat
    16441 tgtgtcatcg ggtcacattc aaaaatcgta gacagtagga ttgaggatca cgtcgtcatt
    16501 gaaaactcag tcattgaaag cagccatgtc aagaaacatg cagatgtggg accttatgca
    16561 catttacgac caaaagctga aattggcgaa aacgtccata tcggaaactt tgtggaagtg
    16621 aaaaatgctc aaatcggcaa aggaactaaa gtcggtcact tgacttatgt tggtgatgct
    16681 acgctgggag aagaaatcaa tgtcggatgt ggtgttgtct tcgtcaacta tgatggaaag
    16741 aacaaacatc atacgactgt cggcgaccac agctttattg gttctagcac gaatatcatc
    16801 ggacctgtcg aggtagctaa aaacagttcg attgctgccg ggtcaacaat tacagacaat
    16861 attccagaat atgcgctggc aattgcgaga gcaagacaag tcaataaaga aggatatgca
    16921 aaaaaactcc cttatctaaa ctaagacttt tgcaaaatca cttgatttat ttaaaaaata
    16981 tgactactat ttattaggta gaattctaga gttgtgaaaa agaaagtgga ggtccccatg
    17041 tcaaaacatt actttgaccc aagattaaaa atttttgcgt taaattccaa tcgcccatta
    17101 gcagagaaaa ttgcagcagc agtcggcgtc gagttaggaa aatcatctgt tactcaattt
    17161 agtgatggag aaattcaagt aaacattgaa gaaagtattc gtggatcgca tgtctatgtt
    17221 attcaatcaa cgagcagccc agtcaatgat aacttgatgg agttgctgat catgattgac
    17281 gcactgaaac gagcaagtgc aaaaacaatc aacgtagtca tgccttatta tggctatgcg
    17341 cgtcaagacc gtaaagcacg tgcacgggaa ccaatcacag cgaaattagt agcaaacatg
    17401 attcaaaaag ctggtgcaac aagaatgctg actttggacc ttcatgcagt ccaaatccaa
    17461 ggtttctttg atattccagt cgatcatttg atgggtgcgc cacttattgc caattatttc
    17521 ttagaaaaag gaatcaaagg cgatgatgtt gtcgttgttt caccagacca tggtggtgtc
    17581 acacgggcac gtaaattagc ggaatttttg aaatctccga ttgcgatcat agacaaacgc
    17641 cgtccaaaag caaatgttgc agaagtaatg aatatcattg gacaagtcaa aggcaaaact
    17701 tgtgtactga tcgatgatat gatcgataca gcaggtacga tcacattggc tgccaatgct
    17761 ttgaaagaag ctggtgcaac tagtgtgtat gcttcatgta cacatccagt attatccggc
    17821 cctgctttac aaagaataga agattcagct atcgagcgtc tagttgttac agactcgatc
    17881 tatcttccag aagatcgtaa aatcgacaaa atcgatgaag taagtgttgg cggactaatg
    17941 ggagatgcaa tcaaacgtat ccatgaaaac aaaccagtta gtccattgtt tgaaacaaaa
    18001 aataaataat aaataaactc acaaagggga gtccaattgg gctgagattg aatctatttc
    18061 taaacccttc gtacctgtat cggttatgcg agcgtaggaa ttgtgaataa acagctgttt
    18121 gactattttc gaaagctgct tcctcctttg tgactttttt attcaaagga ggaagttttt
    18181 ttatgggaaa aaatagagtt tgggtagaag gaacgattgt tgcagcgtta gcgatggtgt
    18241 tgtcgttgat tccgatccag atcggcagca gtttttctat ttcattaggg cagattccat
    18301 tgactctttt tgcgttaaga agagggacaa aggcagggat gctggcaggt ttcatttggg
    18361 gcatcctaca ttttccgttg ggacaagttt attatttaag tgtcgttcaa gtgttgattg
    18421 agtacccgat tgcctatact tttgcaggcg cagcagggct catggcttca aatgtccaaa
    18481 aaggctatgt agacgataat acgaaacaaa ttagaaaaag catcgtgttt ggtgcattga
    18541 ttggcgcact ttccagatat ttttggcatt ttattgcagg agtggttttc tggggagcgt
    18601 atgcactatg ggggatgaat tcttggctct tttctttcgt aatgaatggt gccagcgggt
    18661 tgagtacagc tattgtaacg atcatcgttt tattagggat ccaacaaacg actccttcga
    18721 tttttgtgcc agataaacga caggagctgt aaataaaaaa gaagcaagaa acttgagaca
    18781 gaatcatgtc ctagtttctt gtttcctttt tattttttga taagaaatag atctacttca
    18841 atggcagata agaaagatcg gcatcttcta ccaattgcca gtgtcgatcg gtgacagagt
    18901 agattaaagt agcgatgatt tcctcttctc gataccgggt gccttttaat ttccaaatat
    18961 aatcttcaat cacatagatt gggatacgat atttaccatc gatcaaacca acttgatcaa
    19021 aagaaaaaat gatcccttcc tgcatcaaat tacgcatttc tttcaataat tccacactga
    19081 ttttttcaac aggtgtttct tttttgaaaa aagatttttt tacattgatc ttatctgaca
    19141 agatcgaact taatttacta gcaaaaatcc tataaaattc atctaaatag cgataataaa
    19201 tccgattcag ctgatcatta agatcgaagt atacaaaatt attctgtagt ttataaaaaa
    19261 acggcgaatg aagatgtgtt ttcatatgac caaaatataa taattctgaa atttctaaag
    19321 gtgtcaattc tttcagcatc gttaaatcag taaagtcgat ccacttcaat tcatcgaatc
    19381 cacgcctgtt acgagaagag gaaaagaaac gctgaatatt ttctgtacca cggattactt
    19441 tcagccctgt atgggattca tattctccag cagaagcaga tggatcaagc aagagcaagt
    19501 tttgaggaaa atgcacgata ctgtttgcaa agtcagtagc agtcagccct tttgttaaaa
    19561 cagcattact tgtggtatca atatgtacat aaactgactc agtcatcatg ttcctcctct
    19621 tatttcctct taatctcttt tattttacaa catttgcatt aaaaaagctt gtttctttta
    19681 ttttaaaatt ccattacaaa atcacgtttt cattgaaaac actctcattt tcaattaatt
    19741 tttgtgattt ttgtattttt tatttaaagg atgttttatg aattgttttc ttatggaatc
    19801 tacaaagtcg aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa
    19861 aggagagcgc gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc
    19921 ctatccagca agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg
    19981 ctttttggcg ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg
    20041 tctattttac tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag
    20101 gtgaaacttt tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta
    20161 ttaaagaaaa ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt
    20221 ttcataaaaa cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag
    20281 cacttgtttt aggtggagga ggagcacacg gtgcttatca gattggtgtg tggcaggcgc
    20341 tgaaggaaca tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg
    20401 gcgcattgat tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga
    20461 caagacaagt gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct
    20521 atcgagaaag acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc
    20581 cattggaaaa gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa
    20641 tacgtttata tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc
    20701 aacagacaaa aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg
    20761 ctatggctta tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa
    20821 tacctattga tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag
    20881 ggccaggccc agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa
    20941 cattgtggac actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac
    21001 aattaggcta tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat
    21061 ttcattctgt taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag
    21121 aaattcagtt agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt
    21181 tatataagaa cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa
    21241 aaaaacattt tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct
    21301 gccaacaaga tataaaaagt atgccggatg atttattggc acaaattggt caattaagta
    21361 ctgaagaatg gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa
    21421 ctgtttattt tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc
    21481 ttaagcaacc agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac
    21541 catggcacaa gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa
    21601 caaggggtgg cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga
    21661 tcttcgagat tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga
    21721 agagtttgaa caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa
    21781 tgaaaagtat gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata
    21841 ttgaaataaa aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc
    21901 tgaactttgt tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa
    21961 tcgaagtatt tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact
    22021 gggaattgat tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta
    22081 gtcaaaatca agtgtcaatt agtcggatgg cggaacgctt cgcgctggac gaaaacttcg
    22141 taacggtaga agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata
    22201 cattgaaaag cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag
    22261 ctttagtgtt gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt
    22321 ttgtagaaat ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc
    22381 ttggagcgaa agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg
    22441 gagatattca cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta
    22501 tcgaagcaca atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg
    22561 aaatcaacac gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt
    22621 ctaagacgat cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt
    22681 agagataatg aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa
    22741 acgggatatc ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta
    22801 taatgagaac gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa
    22861 atttattgtt cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg
    22921 aaaagatgag atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg
    22981 ctgaacaaag aaatacaata aataatcaga attggatttc ttcgcttagc ttctttgctc
    23041 taggaataca tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata
    23101 agtgatgttc aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa atgtgagaaa
    23161 ctattttcac aaaatttttc ttcatactcg aaactaatcg gcttcttcac gacctcttat
    23221 acacggcgtt caaaagcttg catctgcgat aataacccca accaaacgaa aaacatgaga
    23281 agctgtttat gtttggctgt tcttattact tgatgcagga cagctttttt acgacctctt
    23341 ggtacagggt agctttttta ccaatttcta gctataatta aagataacct tgaaagttgc
    23401 agaaaaaaag tgcaaaataa acgataagta caaaaagact agttgaaagg acgtcgtcat
    23461 gtcagagcgt ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa aaggcacagt
    23521 acgaaaagca atttttgaga gtgaggataa tgattttcag tattcgatct caatgactac
    23581 ccgtaaaatg cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa gcaaagaaga
    23641 gtttgaatca atgatcgaag ctggagagat gctggaatac gcagaatatg tcgggaatta
    23701 ttatggaact cctttgtcat atgtcaacaa gacattggat gaaggaaaag atgttttctt
    23761 ggagattgaa gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg gtgtattcat
    23821 ctttttgact ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc gaggaacaga
    23881 tgcagatgaa gtaatcgaag aacgaatgaa agttgcaaaa gaagaaatcg aaatgatggc
    23941 tctttacgac tatgctgttg taaatgatga agtgccgcta gcagttgaac gaatcaaaaa
    24001 cattattgcc agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata ttaaaatgtt
    24061 aaaggagatg tagtcttatg atgttaaaac cttcaattga ctcactatta gatcgtgtaa
    24121 attcaaaata ttctctagtg attttagcaa gtaaacgcgc gcatgaattg gatgcaagtg
    24181 cacagcctac tctagattct tttgattcag taaaaagcgt cggtcaagct ttagaagaaa
    24241 tcgatgcagg aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa cgtttgaaga
    24301 tggaagaaga ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg gaatcacgta
    24361 tccgtgaaga gcaaaaaatg taataaagaa aaatagagcg aacatctaaa aagaaaagca
    24421 aacccagctg tttccttttc aaacattcgc cacttattgt ctggtagaaa aggctgtgtc
    24481 tgaacttttt gacacagttc ttttttttct tatctgctgt taattttttg ttaggatttg
    24541 gcagaagata agacaaatcc atcgatctct agtaaaatag gcaaggaact ttttgtcgct
    24601 tgataagaaa agacaaaaaa aggaggaaac gaaccatgac attaacagct gaagtcattg
    24661 tggacgtacc tactatgcag accgatcagc cattcactta tttggttccc tctgaaatgg
    24721 aaacagcttt gcaagtcggg atgcgagtag aagtcccttt tggtaatggc aatcgtcatg
    24781 tccaaggatt tgttatggct atccaaaaat cagaagaatc agctaatcca tctttgaagg
    24841 cagttattcg tttgctagac cttgctccgg tggtcaatga agaattactt tcattagctg
    24901 attatatgaa aaaaattact tacgcattca aaatcacttg tctacaaacg atgcttccga
    24961 gtgtgatgaa agcagaatat gataaattga tctatccttt ggcagacaca ccagaagttc
    25021 aggtattgtt tgatcagcga gaagtgattt cctggaagga agcagaagaa gaaggcagcc
    25081 tttctcaatt gattcgctgg cgccaagagc aattagtaga tatcaaatat gaagtccata
    25141 cacgaaataa agtaaaaacg attcgtttgg tccgttcttt gttaactgaa aagcaaatcg
    25201 aagaagaatg ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt cttttgtgtt
    25261 tatctgaact gagccaagaa gaaccaattg cttatttcaa aaataaagga atcagtactg
    25321 ctgtcttgaa tcaaggaaaa gaaaagggct ggctggagtt tatagaatca gaacgttatc
    25381 gcgaccctta taaagaccga gtatttgacc aaacgacggc attggaactg aatgcagaac
    25441 aaaaaaatgc tgtcgaacaa atcatcacag caggtcagca gcaaaaagat caggtctttt
    25501 tgctggaggg aatcactggc agcgggaaaa cagaagtata tcttcaggca atcgctgatg
    25561 ttttgtcaga aaataaaacg gctattatgt tagtgcctga aatcgcattg actccgcaaa
    25621 tggtcgagcg attcaaagga cgcttcggag aatcagttgc ggtcttacat agcggacttt
    25681 cccaaggtga aaaatatgat gaatggcgca agattgagcg ggaagaagcg caagtagtag
    25741 taggcgcacg atctgctatt tttgccccgc tgaaaaatat cgggctgatc attatcgatg
    25801 aagaacatga atcaagttac aaacaagatg agacaccgcg ttatcatgcg agagatctag
    25861 caatttggcg cagtaaatat catcattgcc caatagttct aggaagcgcg acgccctctt
    25921 tagaatcaag agcacgagct caaaaaggcg tttatcagtt attgcagtta aaccaccgag
    25981 ccaaagcagc tgcacagctt ccagctattg agatcgtaga tatgcgtgaa gagtttcaga
    26041 atcatcggac ctctactttt tctgctaatt tgcaggaaaa aatacaaaat cgattagaca
    26101 aaaaagaaca aactgtccta ctgctcaatc gtcgaggata ttcgtctttt gtgatgtgta
    26161 gggactgtgg atttgttctt ccatgtccta attgcgatat ctctcttaca ctgcatatgg
    26221 atacacgttc aatgcgctgc cattattgcg gacatgaaga acctattccc aatcgctgtc
    26281 ctaattgtgg cggcaataaa atccgctact atggaacagg tactcaaaaa gtagaagagg
    26341 aactccgaga actttatccg caagctagga ttttgcgtat ggatgtagat accactcgaa
    26401 gaaaaggggc acacgaacag attttacaaa aattcggtgc aaaagaagca gatatattat
    26461 tagggacaca aatgattgct aaaggtttgg actttccaga agtgacatta gttggggtgt
    26521 tgaatgctga tacctccctg aatttaccgg attttcgttc tagtgagcat acattccaat
    26581 tgcttaccca agtttcagga cgagcagggc gagcggaaaa agcaggcgaa gtcgtgatcc
    26641 aaacattcaa tcctcaacat tacgccatag aattggctaa aaaacaaaat tatgagcagt
    26701 tttatcagca ggaaatgcat gtacggcatc gtggaggcta tcctccttat tactttactg
    26761 tgaaaataac cgccagccat cctgaagaac aagtagcggc aaagaaaatc tttcagattg
    26821 ccaatcagct aaaagaagtg ttaagccctc aaagcttgct attagggcca acgcccagca
    26881 tgattttgag agtgaaaaac cgatattatt atcaactaat catcaaatat aaacatgaac
    26941 cgaatcttcc tcatgttttg gatgaaattt tgaatggaag ccagaaagaa cagcgacaag
    27001 gtctttttgt ggcaattgac aacgaaccat taaactttat ttaagaaagg gccaaattat
    27061 gcgctatcct atattgatcc accctaatga taaattaaaa agaactgctc agccaatcga
    27121 tgtaatcacg gacgagacaa tcgctttatt agacaattta tatgagacaa tgattgcgaa
    27181 tgacggaata gggatcgctg ctcctcaagt aggacaaaac aagagaattg ctgtgatcga
    27241 agtagatgaa ggcgagaaat ttgaactgat taacccagaa atcatcgaag caaaaggtga
    27301 aagccttgac gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg tcaagcgagc
    27361 agatgaagtg accgtgcgtt attatgatcg agacggagaa gagatagaag tgacggcttt
    27421 tggctattta gcccgtgcct tccagcatga aatcgatcac ttagacggta tcttgttcat
    27481 tgagaaaatg atacagcaga tcccggaaga agaacttgaa gaatatatgg aggaacatct
    27541 tgatgactaa gctagtattt atgggcacac ctgctttttc tgtgccgatt cttgaagggc
    27601 tgatagaatc aggttacgag atccaggcag tggtaacaca gcccgaccgt ccagttgggc
    27661 ggaaacgagt aattacgccg acaccggtaa aagaagcggc tctgaagcac ggtatccgtg
    27721 tgctgcagcc agagaaaatc tccggttcgc cagaaatgga agaaatcatt gaattggtgc
    27781 ctgatgtgat cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta ttgcaagtac
    27841 caaaattagg ggcaatcaat gtccatgctt ctttacttcc taaatatcgg ggaggtgcgc
    27901 ctgttcatta tgccatcatg aatggggaga aagaaactgg tgtaacgatc atggaaatga
    27961 tcaaaaaaat ggatgcaggc ggaatctatg cgcaagaaag cataccaatc actaaacaag
    28021 atgatgtagg gacgatgttt gaaaagctta gccttttagg aagaaagcta ttgctagaaa
    28081 cattgcccaa tattttggat ggacaaaaac cagtacctca agacgagtca gaagcaactt
    28141 tttcgccaaa catcacacgg gaacaagaag caatcgattg gcataaaact gcagaagaaa
    28201 ttgacaacca agtacgcgga atgcgtcctt ggccaattgc attcacgacc tatgagggca
    28261 tacgctggaa attattatcg gttgaagtat tggcagaaac tacagacaaa gcacctggta
    28321 cgatcatccg gaaagacaaa aaaaatctat ggattgcttg cggtaaacag accgtcttag
    28381 ccatcaatga attacagcca gctggtaaag gaaaacaggc cgtacatgat ttccttaacg
    28441 gaagcggtca gcaagttcag attggtcagc aggtgaaata atgaaaaaaa tccctaaaaa
    28501 aatgaaaaaa tcagtacgat atatggcatt actgacatta gagcgagtga acaagggtgg
    28561 cgcctactcg aatcttttgt tgaatgagat gatcaaacaa agcgagctaa atcaaaaaga
    28621 tgtgggttta tttacagaat tgatctatgg tacgattagc catcagcgac tattggaatt
    28681 ttacgttgca ccattgatcg ctaaagcaaa aaaagtggat gactgggtca agacgcttct
    28741 ttatctttct gtataccaat tagaattttt agataaagtc cctgcacatg ctattttaaa
    28801 cgaggcggtc gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat ttgtaaatgg
    28861 tgtcttgagg aattatcagc gtcaaggagc accagatctg caaggcattg ctgatcctat
    28921 cgaaagactt tctgtcaaaa tcagcctgcc tctatggatg actaaacgac tggtggatca
    28981 aatcggatac gaagaaactg aaaagctcgg tttatcgcta tatcagccta gccatgctag
    29041 tgcccgagta gatacacgcc gactttccag agatcaggcc atcactgttt tgcaagaaga
    29101 agagatcaca gctgaaaaaa gccaagtcag tccatacggt atagtagcga aaaaagggca
    29161 tttagcttct agctcattgt tccacgatgg cgtcatgacg atccaagatg aaagttcgat
    29221 gcttgtagcg ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg cctgtgcagc
    29281 acccggaggt aagacgacgc atattgcttc ttttttagat ccgtcatgcg gtggagaagt
    29341 ggtcgcttta gatgtacacg aacacaaagt caaactcatt gaagataatg cagcacgtct
    29401 aggtgtctct tctgctgtct acgctcaaaa gatggatgct agagaagtca gtaaacagtt
    29461 cgaagcagag cagtttgatc ggattttagt agacgcgcct tgttcaggtc ttgggctgct
    29521 tcggcgaaaa ccagatattc gttataaaaa acagccggat gaattagcga atttaccaaa
    29581 aatccaattg gaaattttag aaagctgcgc accaacttta aagtcttccg gtattctgac
    29641 ttatagcact tgtacgatct taaaagaaga aaaccaagac gttgtttctg ctttcttgga
    29701 aagacaccct gattttgaga ggattgatgt tttgaccaat cctatgctac aatcatcaat
    29761 cgaagagaaa atgttgactt tgtatccgca tcagttttac acagatggat ttttcatttg
    29821 ttgtttgcgc aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat cagatgtagg
    29881 aagacgacgt aacacaaatc aagactatgc cagtgtattt accagccgtt caggtctcaa
    29941 gcttgctgta ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg ccagtcagat
    30001 ggcggtaacg aatctaggcg aagcttggga aaaacaagaa gctgttgacg atgaaaaaat
    30061 cgcccaatgg ttcattcaat cgattcaaga agaaaatatg ctgatttatc agcgtggaca
    30121 agaacagccg gaatataacg gtatgggaac aacaatcgta gcggccgctc tgtcagatga
    30181 ctggttcacg attgccaatg ttggggatag cagagcttat ttgattcgtg aagataaaat
    30241 catgcaattg acggaggatc attctttagt caatgaacta gtcaaatcag gagaaatcag
    30301 cgaagaaatg gcagtgaacc atccaaggaa aaatatttta acacgttctg tcggcatgcc
    30361 tggaacagtc gaagtagatg tttctactca tctttggcaa ctaaaagatc gactgctgtt
    30421 atgttcagat ggcttgacga acatgctgtc tgaagaagaa ataggaacga tcatcaatca
    30481 agaaggccca ttgtctcata aagtaactga gctgattgat cgtgccaatg aagcaggcgg
    30541 agcggataat atcaccgttc ttcttatcga atataaggag gacagtgtat gatcgagttg
    30601 ggcaaaaaat taaatggccg ttatcaaatc actggaaata tcggtagcgg tggtatggca
    30661 aatgtatttt tggcgcacga tttaatcttg gatcgtgatg tagcagttaa agtcttacga
    30721 tttgactttc aaaatgacca gactgcgatt cgacgatttc agagagaagc tttagcagca
    30781 acagaactcg ttcatccaaa cattgttagt gtttacgatg ttggtgaaga agacaacatg
    30841 cagtatctgg tgatggaata cgtcaaagga atggacttga aacgatatat ccaaacacat
    30901 tatcctgttc cttatgaaac agcagtaaat attatgcagc agatcctttc tgctatttct
    30961 ttagctcata accatcaaat catccaccga gatttgaaac cccaaaacgt actgatcgat
    31021 aatgaaggtg ttgtaaaaat cacggatttc gggattgcta tcgctttatc tgaaacatcg
    31081 atcacgcaga cgaatacgat gctaggatct gttcattatc tgtcccctga gcaggccaga
    31141 ggaagtatgg caacgaagca gtcagatatt tatgctttag ggatcatcct ttatgagatg
    31201 ttgacgggta gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt aaaacatttt
    31261 caagatgatc ttccatctat aaaagcactt gatccaaatg tgccacaagc actggaaaac
    31321 gtgattttac gggcaacggc taaagaacct gcagaccgtt ataagtcagc cgaggaaatg
    31381 tctgatgatc tttctacagt attatcacca gctcgtgcaa acgaagaaaa atggcagcct
    31441 catgtgatgg ataatgaaac aaaagttatc acacccctta cggaagatgc accaatgcct
    31501 gattcattca aatctatgcc tctgccaaaa gacaagccag aaaacgaaac cttaccagag
    31561 gaggaagaag ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg gctgatccta
    31621 ttagctattc ttgctgtttt aggaatcgga acagctgtat tctttgcttc tggcgggcga
    31681 ggagaagttg ttgttcctga tgtttctgat ctttctgaag cttctgctag agaaacactc
    31741 gcaaaagcga aattagaagt agcaacaaaa acagaagaaa ttgcagatga caaaatcgaa
    31801 gaaggtaatg tagtgaagac agacccggca gcgggaacga cagtcaagca aaaacgcgaa
    31861 gtcactctgt atattagtag cggcacgaaa aaaatcaaat tagatgatta cagtggtaaa
    31921 agttatgaag atgccgaagc aagtttgaaa gaattagggt tttctgctga cttgattcaa
    31981 aaagaagagg aattcagttc agaggtagaa agcggaatga tcatttcgca atctccatca
    32041 gaagatacag aagtcgatcc taaaacagac acgattactt tcacagtcag caaaggacca
    32101 gaaacagtga ctttgagtag tcatgtagga gaaaactacg aaactgttcg taattggctt
    32161 gttacacaag gattcaatat caataatatt agtgctactt atgactatag cgacactgta
    32221 ccatcaggtt cgatcatcag tcaggatcca gcctcgggtg aagttgtagc agaagaaact
    32281 tacatcaatt ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat ctccggctat
    32341 acaaaaagcg aagcacaaag ttatttagct agtgtcggtg cagaatatat cgggcatgag
    32401 acttccgaat attcaaacac ggtggataaa gataaagtga ttcgtacagt accaggggca
    32461 ggaacgacaa ttaccaaagg aatggttgtg aacgtaattt actctaaagg accagatcca
    32521 gcgtcttcta gttctgaaaa agactcttca gacagttcta gcagctcaag tagctccagt
    32581 agcacaagca actcttcaaa agaagaatct tccagctcca caagttcatc agctagcacc
    32641 tcagaaagtt caaaataagt gctgaataaa atatatcaga agggaatgcg acattgattt
    32701 gttgcattct cttttttgtg tttctatctc ttttgatcaa ttttcaagcc atatactttg
    32761 agaagtttgc atggaataac acctactttc ctcttcgtat ggtaaaataa acaaaaacgg
    32821 aggtggcact tatcaaagga caaatcagaa aagcaatcag cggtttttac tatgtatacg
    32881 caaacggaga gacctatcaa acaagaggaa gaggaaattt ccggaatcgg aaaatcacgc
    32941 cgcttgtcgg agatcaggtg atctttgaga gtgaaaatca aacagacggc tatcttctgg
    33001 aaatcttgcc gagaaaaaat caattagttc gcccaccagt cacaaatgtt gatcttggaa
    33061 tcattgtcac aagtatcgta gagcctgcat tttcttataa tctacttgac cgctttctag
    33121 ttaccttgga atacgagtcg atcgaaccga ttattttttt gacaaagacc gacttgtcag
    33181 acgacccaga aaaaatcgaa gagatcaagc aaacctatac taaaataggc tatccggtga
    33241 ttgtacccaa tcatacgggt gatacgacag aactgatccg ttacttccca gaacggctat
    33301 cggtctttat gggtcagtct ggagcaggaa aatcgacgct actgaatcaa atatcaccgg
    33361 aactgaattt agcaacagga gagatttctg atgcacttgg aagaggacgg catacaacac
    33421 gtcatgtgga actgattcct atctatggcg ggttagtagc agatacacca ggatttagct
    33481 cgattgactt tttgacaatt gaagcaggag aactgccgag acaatttcct gaatttgtag
    33541 aagcttctgt ccattgcaaa tttagagagt gcatgcatgc taaagaacct ggctgcgaag
    33601 taaaacatag agtagaaaca ggtggtatcg cacagacacg ttacgacaat tatttgcaat
    33661 tcttacaaga aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt aaacgaaaat
    33721 aggaggaaac aagaatgaag atagcaccat ccattttgag tgcagatttt gccaatttac
    33781 aaagagacat tgagttagtg gaaaaaggag gcgcagatta catccacgta gatgtaatgg
    33841 atggccaatt tgtcccaaat atcacattag ggccaaatat tgttcaggca atccgtcctg
    33901 ttactaaatt accgttagac gtccatctga tgatcgttga tccagaaaac tacattccag
    33961 catttgcaaa agctggagca gatatcatta ctgtccacgt cgaagcaact cctcatattc
    34021 atcgggcact tcaaatgaca aaaagcttag gagttaaagc tggagtagtt atcaatccag
    34081 gaacaccagt ctccatgatc aaacatgttt tgcctttggc tgatcaagta ctagtcatga
    34141 ctgtcaatcc tgggttcggc ggacaaagct ttattgaaga aacagtagaa aaaattacgg
    34201 aactcgctga tctacgtaga gagaacaatt ggacttatgc aatcgaagta gatggcggaa
    34261 tcgttccaga aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc gttgcagggt
    34321 catatatcta tggagccgaa aatccagtcg gacaaatcga acgattaaaa gaggcattag
    34381 actaaatgaa aattttatta gctgctggag gtaatccgca aaactggccg cagtctctgc
    34441 tgtccaaata cgatgcctat gtcgggatag accgtggtgc gctgtacctg cttcaagcag
    34501 gctacccagt agatctggca gtgggtgatt ttgattcatt gtcgaacgaa gagcagaagg
    34561 aagtatttgc aaaagccaaa gagtataccc gttcccctgc agaaaaagac gatacagata
    34621 cacagttagg tatcttaaaa acatttgaac gttacccgca agcggaagtc actttgattg
    34681 gagctacggg cggaagattg gatcatcttt tagccaatct ctggttaggt atggaacctc
    34741 gcttcaaacc ctttgcccat cagcttatta tcagagattg tcaaaattat ttgtcttatt
    34801 atttgcctgg agagcatata gtaagacaga taccagatat gaagtattta gcttattgct
    34861 gcttgactcc cgtctcgaat cttacattaa aaggcagcaa gtatgtattg gaaaataaag
    34921 aagtagaata tccgacatct tatgcaagta acgaatttct ttcagatacc gcagaatttt
    34981 ctttttcaga tggtatgatt gcagttattc aaagcaaaga ttgatccata aacaaataaa
    35041 aaaatttgtc tttcaggcta aaaatgacag aatctattat ttgctaaaaa aaagcttaag
    35101 aggagcaact taccttttgt ttcacggcat ttagtgataa aattgaacac aagagtctaa
    35161 ttaattcgag gtgtctttgt gaagaagttc aatcctaata aaaatattat tattaccttg
    35221 gttattgtta tcatcgtggt gacgatacta agtgtgaccg ctgcgcagcg agcgaatgaa
    35281 ggaaagccca atttcttcca atccatcgtt aatgacagtg ttgcaatggt tgataaaaca
    35341 atctctgccc cagtacggtg ggtacgaaat ggagtagatt ctgtccataa tcttttttca
    35401 acttatgccg aaaatgagcg gctaaaagaa aaaatcgata gctatgatga cgtttcacag
    35461 caaaacaaga attacgagag agaaatcgaa gcgttgaaaa aagaacttga gttgaatcag
    35521 acgctgacta gttacgaaaa agtaacggca aatgtcatta ctcgttcgcc tgacagttgg
    35581 caggacatgc tgatcgtaga caagggttct aaagacggta tcgaagcaaa tatggcagtc
    35641 atgtcgcaaa aaggattgat tggccgagtg atcgaggtca atacggcttc gtctaaaatc
    35701 gaattactgt catcctctaa tgaaagctcc aatcattttc cagtacgggt atcttcggct
    35761 aatggcgaag cgtttggttt gcttaaaaac tatgatgaaa agctccatgc cttagtggtg
    35821 acccaattaa ctggtgatac ggatatcaaa gaaggggatg ttgtccagac atccggtctt
    35881 ggagggaatt ctccagctaa cttgccgatc ggtacggtta ttaaaacgaa accagatagt
    35941 tatgggctgg atcgggaagt ttatgtgaaa ccttatgcag aaatgtatga cgtgtcagtt
    36001 gtgacgattg tccaacggct agcggaggat tagtccatgt tgaagaaaga aacaatgaag
    36061 tactatctgc caatcgtttt gttctttttg atgttgatag atggtcattt aacaagaatg
    36121 ctaggggagt ggtcgaaagg cacctatatg tcaaatgccc actttctgat attggcatta
    36181 ttatgttgca gtatggcgtt tgaaaaacgt tatttactga ttaccacgat tgttctcggg
    36241 gctatctatg acgcttacta tattggcgtt atcggtatct atgcagtagc tctcccttta
    36301 attgtatggt tgatgtatgt aatgaaagac gttatccatg tcaacatctt tactgaattt
    36361 ttcagtatga tcatctttgt cacgggttat gaattgttta cgatggtggt ccagttgatt
    36421 tttaaattag cagtagtaaa taacacgtat tttattacaa ggtttttagg acctacactg
    36481 ctgttgaaca tgattatatt tgtattattc atttttccct ttaagaaatt attcagtgat
    36541 gaataatttc tttttttatt tttaggtcga aagcgaaaaa gaatcgttgt tatcttaaga
    36601 aatgctagac ttaacgcggc tttttcatga tcattttctg aaacgaacag ttttgtcatt
    36661 tttaaaagtt gttttgaaac atttttgtaa cataactatt atatcgttga cacactgcta
    36721 tggtacaatt tttatatcgt taagaaagac ttaaatatat ttagatattt attaaattaa
    36781 gccagtcgga ggaatgaaga gtgaaaaaga gtttaatatc agcagtaatg gtatgttcaa
    36841 tgaccttgac tgccgtagca tcaccgatcg ctgccgcggc ggacgatttt gattctcaga
    36901 tacaacagca agatcaaaag attgcagact tgaaaaatca acaagcagat gcacaatcac
    36961 aaatcgatgc cttagaaagc caagtttctg aaatcaacac acaagcacaa gacctattag
    37021 caaaacaaga tacattgcgt caagaatctg cacaattagt aaaagacatt gcagatttac
    37081 aagaacgtat tgaaaaacgt gaagatacaa tccaaaaaca agcacgcgaa gcacaagtaa
    37141 gcaacacaag ctcaaactac atcgatgctg tattgaatgc tgattcatta gcagatgcta
    37201 ttggacgcgt tcaagcaatg acaacgatgg taaaagcaaa caacgacttg atggaacaac
    37261 aaaaacaaga taaaaaagct gtagaagaca aaaaagctga aaacgatgct aaactaaaag
    37321 aattagctga aaaccaagct gctttggaat ctcaaaaagg cgatctttta tctaaacaag
    37381 ctgatttgaa cgtactgaaa acttcattag ctgctgaaca agcaactgct gaagacaaga
    37441 aagctgactt gaaccgtcaa aaagcagaag ctgaagcaga gcaagctcgt attcgtgaac
    37501 aacaacgtct agcagaacaa gctcgtcaac aagctgcaca agaaaaagct gaaaaagaag
    37561 cacgcgaaca agcagaggct gaagcacaag ctactcaagc ttcatctact gcacaaagct
    37621 cagcaacaga agaatcatca gctacacaat cttcaatgac tgaagaatca tcatcagcta
    37681 cacaatcttc agcaacagaa gaatcaacaa cacctgaatc ttcaacagaa gaatcaacag
    37741 cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca gcaacagaag
    37801 aatcaaaaac agtgcctgaa tcttcaacaa cagaagaatc aacaacacct gcgcctacaa
    37861 caccatcaac agatcaaagt gttgatacag gaaacggcac aggaagtagt actccggctc
    37921 caacgccaac accaacacct gaacaaccaa aacctgtaac acctgctcca gctccttctg
    37981 gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa atatattggt actccatatg
    38041 tttggggcgg taaagatcca agtggctttg actgctcagg gttcacacgc tatgtttaca
    38101 tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc tcaagaatca gctggaacaa
    38161 aaatttcagt ttctcaagca aaagctggag acttgttatt ctgggggtca caaggcggaa
    38221 cttaccacgt agcgattgct ttaggcggag gacaatatat ccatgctcct caaccaggcg
    38281 aaagcgtaaa agttggttca gttcaatggt ttgcccctga ctttgctgtc agcatgtaag
    38341 aatgattaat caaaaagagt tagcccattc tggactaact cttttttt
//
LOCUS       DACEKG010000020        38334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEKG010000020 DACEKG010000000
VERSION     DACEKG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.020"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            161..391
                     /locus_tag="IW664_000932"
     CDS             161..391
                     /locus_tag="IW664_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0848313.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IW664_000933"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IW664_000933"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0848314.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IW664_000934"
     CDS             1802..1978
                     /locus_tag="IW664_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0848315.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IW664_000935"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IW664_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0848316.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IW664_000936"
     CDS             2593..3060
                     /locus_tag="IW664_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0848317.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IW664_000937"
     CDS             3145..4029
                     /locus_tag="IW664_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0848318.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IW664_000938"
     CDS             4146..4379
                     /locus_tag="IW664_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0848319.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IW664_000939"
     tRNA            4467..4547
                     /locus_tag="IW664_000939"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IW664_000940"
     tRNA            4554..4625
                     /locus_tag="IW664_000940"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IW664_000941"
     CDS             5140..5847
                     /locus_tag="IW664_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0848320.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IW664_000942"
     CDS             complement(5907..6551)
                     /locus_tag="IW664_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0848321.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IW664_000943"
     CDS             6678..6965
                     /locus_tag="IW664_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848322.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IW664_000944"
     CDS             7073..7393
                     /locus_tag="IW664_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0848323.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="IW664_000945"
     CDS             7474..8787
                     /locus_tag="IW664_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0848324.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="IW664_000946"
     CDS             8784..9503
                     /locus_tag="IW664_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0848325.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="IW664_000947"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="IW664_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0848326.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="IW664_000948"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="IW664_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0848327.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IW664_000949"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IW664_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0848328.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IW664_000950"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IW664_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0848329.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IW664_000951"
     CDS             14940..15833
                     /locus_tag="IW664_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848330.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IW664_000952"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IW664_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0848331.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IW664_000953"
     CDS             17770..18627
                     /locus_tag="IW664_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0848332.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IW664_000954"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IW664_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0848333.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IW664_000955"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IW664_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0848334.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IW664_000956"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IW664_000956"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848335.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IW664_000957"
     CDS             21383..22141
                     /locus_tag="IW664_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848336.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IW664_000958"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IW664_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0848337.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="IW664_000959"
     CDS             23069..24100
                     /locus_tag="IW664_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0848338.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="IW664_000960"
     CDS             24097..24864
                     /locus_tag="IW664_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0848339.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="IW664_000961"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="IW664_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0848340.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26832..27149
                     /locus_tag="IW664_000962"
     CDS             26832..27149
                     /locus_tag="IW664_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0848341.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27149..27499
                     /locus_tag="IW664_000963"
     CDS             27149..27499
                     /locus_tag="IW664_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0848342.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="IW664_000964"
     CDS             27666..28604
                     /locus_tag="IW664_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0848343.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="IW664_000965"
     CDS             28619..29449
                     /locus_tag="IW664_000965"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0848344.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="IW664_000966"
     CDS             29493..30518
                     /locus_tag="IW664_000966"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0848345.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="IW664_000967"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="IW664_000967"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0848346.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="IW664_000968"
     CDS             31596..32036
                     /locus_tag="IW664_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0848347.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="IW664_000969"
     CDS             32039..32641
                     /locus_tag="IW664_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0848348.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32704..33807)
                     /locus_tag="IW664_000970"
     CDS             complement(32704..33807)
                     /locus_tag="IW664_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0848349.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34017..35018
                     /gene="ccpA"
                     /locus_tag="IW664_000971"
     CDS             34017..35018
                     /gene="ccpA"
                     /locus_tag="IW664_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0848350.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35373..35768
                     /locus_tag="IW664_000972"
     CDS             35373..35768
                     /locus_tag="IW664_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848351.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35768..36019
                     /locus_tag="IW664_000973"
     CDS             35768..36019
                     /locus_tag="IW664_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848352.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36062..36457
                     /locus_tag="IW664_000974"
     CDS             36062..36457
                     /locus_tag="IW664_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848353.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36454..37710
                     /locus_tag="IW664_000975"
     CDS             36454..37710
                     /locus_tag="IW664_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ0848354.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37722..38045
                     /locus_tag="IW664_000976"
     CDS             37722..38045
                     /locus_tag="IW664_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848355.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38038..38304
                     /locus_tag="IW664_000977"
     CDS             38038..38304
                     /locus_tag="IW664_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848356.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12859 a         6514 c         8261 g        10700 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 gggggaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gtttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcatgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 gatcgatgac tatgagcgta cacacgcttc tacgattgcc gttatgcaag ttcctcacga
    31021 tgaaacttca aaatacggaa tcatcaaccc aggggaagta ctggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tgggcgttac ttattgactc cagaaatctt tgatgtatta tcagaacaac aaccaggtgc
    31201 tggaaatgag atccaactaa ctgacgcaat cgatacgttg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttatga cgttggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctga cacatccaga agtaggggca cctttgcgtg aatacatcat
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc aatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata atcttttttt ctaacgagct tcagcggcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgttttcat cgttcgcgta ttgttgcaag
    31681 tttccaaaac tttagaaaaa gtagaccaga ctgtcgcaga agcaaatacg acaattgaga
    31741 tcgtcacaca agacgtagat cttttatcta gacaagtaga aggcttgcta gtcaaaagta
    31801 atgagctgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcggaac ttaatcattc tagtaaaaac atcgtgacga
    31921 aagtcggcgg aatcggaaaa acgacagcta aagcgactgt tgccggaaaa gtcggtggaa
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataagaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagttgga ggtacagccg cagcaatcgc
    32101 cgcattattg ttagcaccca aatctggtaa agaacttcgg gatgaattgg ctgctcaagc
    32161 agatgatttg atggacatgg cttcagatta cacagatatg gcaaaagaaa aatcatctga
    32221 gttaacaggt attgcaaaag aaaaagcgca aggtctttct caacaagcag gggatctagc
    32281 aggcaatgtt aagcagaagg taagtgaatc agcagaggaa gcaggaagtg aatcagatga
    32341 tatcttgtta aacctcaaag aacaatcagc tgatttatct aatcgtttca aaaaaacagc
    32401 agaagatctg aaaaagccag cagacgaatt aggtagtatt gctaaagata cttcagaaga
    32461 tatcatgatc gatgtaaaag atgctgcaga taaagcagga gagacggtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgacac aaaagaaatg ttgaaaatgg caaaagaaga
    32581 tgcaaaagaa gctaaaaaag aaatgaaaga tacaatgaca gattcgacta ccaatcacta
    32641 agattactca tttctagaat aaaaaaggtg agagcggacc agctctcacc ttttttattt
    32701 tcattactct aaaacaagta attcttttgg tgtagtcgtg aatggcacac aaccatcact
    32761 tgtcacatac acacaatctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32821 ttcaagagag aagcacatcc cttcttcaat cactaagtcg tttccttcta caagagaagg
    32881 gaattcgtgg acggttgttc cgattccgtg acctaaacgg tgattgaaat attcgccgta
    32941 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    33001 gcgaactgcc tcttgtgcgg caagctgtgc ttcaagagtg atgttataga ttttttcttg
    33061 gaactcagtt ggtttttgat aagcaactgt acgagtagca tcactgcaat agccattcca
    33121 aataacgcca agatcgaata acactagttc atttggtgaa accttcgtgc ttcctggtgt
    33181 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33241 aacaccttgt ttttttaatt ggtattcgat ttctgcgata attgcttgtt cagttacgcc
    33301 ttcttttact gttttgaagc caatttcaaa ggcaacatcg gcccatttac cagcttcgat
    33361 cagtttgtct ttttcttcta ccgttttgat cagttgcaga cgctggataa tagaagttag
    33421 atcggtgtaa gaatctgtac ctgtcaagat atcagagata gcatcgaagc gatcgacagt
    33481 cagagcattc ttttcaatac ctacattacc aggttgccca tagcgtgcgc gaagctcttt
    33541 tccgatgatc tcccaaggat tttcgctgtc taagtagcca cgtacatcgt acgcccagct
    33601 gctgttttga gcatcttcaa cttccagagc aggagtgaaa aggaaaggat cttgttccag
    33661 agggataaaa agagctagta cacgctcgtg aggatcactt tcatatccgg aaaagtaagc
    33721 gatgtggcca gggtcgctga tataagccaa ttctacttgg tgttgtgcca tccattgacg
    33781 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33841 caataattgt accactgatt gattaaaaga ccaacgttct taaaactgaa aaaagtaaaa
    33901 attttcatga aaacagagat aaaacggttg caattgttga gattatttga tatcctattt
    33961 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    34021 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    34081 tttcacgtgt cgtaaatggc aatccaaacg tcaaaccagc aacaagaaaa aaagtcttgg
    34141 aagttatcga tcgcttggat tatcgtccaa atgcagtcgc acgtggcttg gcaagtaaga
    34201 aaacaacaac agttggggta atcattcctg atgtcagcaa tatgttcttt gcctctttag
    34261 cacgtggtat cgacgatgtg gcaacaatgt acaaatacaa tatcatctta gcaaactcag
    34321 atggcaacga tcaaaaagaa gtcaatgtat tgaataattt attggctaag caagtggacg
    34381 gcgtgatctt tatgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34441 aaacgcctgt tgtactagct ggttccattg atccagatga acaagtaggt agcgtaaaca
    34501 ttgattatgt gtcagcaaca aaagatgcag taaataaatt agcaaaaagc ggcaataaaa
    34561 aaatcgcttt tgtcagcggt gcgctgattg atcctatcaa tggtcaaaac cgtctaaaag
    34621 gctataaaga agcattaagc gaaaacaatc taccatatag cgaaggattg atttttgagg
    34681 cacaatacaa ctttaaagat ggtctgtctc tagttgaccg gatccataat agcggtgcaa
    34741 cagctgtcta tgtttcagac gatgaattag ccattggtat cttggatggt ttattagacc
    34801 gcggcgtaaa agtaccagaa gaattcgaaa tcatcacaag caacaattct ttactgacag
    34861 aagtagctcg tcctcgtttg acaagtatca cacaaccatt atacgattta ggagcggtat
    34921 ccatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34981 tgccttatgg aatcgaagaa aaaggatcaa ctaaataata aaagatcaat catttaaagg
    35041 ttgttccaaa agtggtcaac ttctggaaca acgtttatac gagaaagggg gttatgacaa
    35101 aattttgtca taaccccttt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa
    35161 accttacgtc catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact
    35221 atcaaaatta ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agataagtaa
    35281 ctctaaaaaa tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat
    35341 ccatcatata taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag
    35401 tcatcatttt gaccattctt actataggaa tcggagggaa attttacatg gacaaaagag
    35461 aaatgcaaag acagaaagat ttactcgcag tagaaaaaca gtccgtcaaa gttctgaaaa
    35521 atacctttgc ggatatcaag gaagtgaaga ttgaaaaatc tgctcgaaat gaaatgacgg
    35581 gttcataccg gatagttatt ctaatgacta ataaacaaga tcaatcgatt tatttttctt
    35641 atagtttttg gaaagaaaga aatgagatag gaagttacgg aatagttgat gaaaaaaagc
    35701 aaaaagaagg aaatacgctc aataaagtta aagtaactta ctctaatgga aatgaggaaa
    35761 gtatttaatg attactgaca aacaatatag tgaattaagt gatgctgttt actggctaga
    35821 tcctaaacat agagactatg tacctgaaat gcaagaaaat ttaagtttta aaattaacgg
    35881 cactatgtat aaaatcctaa aaatcaaaaa ctccttcgac ggcatgcagg cgatggctgt
    35941 agcgccaatc gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctgcaaa
    36001 ctttcgcgta cttaaataat gaaggtgtca atacttacat aaaaagaaag gaggcacgtt
    36061 cttgtcaagg aaaataagaa aactcagctt agccatttta ttaatcgtca tattagggat
    36121 cggagggaaa gtctatatgg acaaaagaga agcacaaagg cagaaagatt tactcgcagt
    36181 agaaaaacag tcagtcaaag ttctgaaaaa tacctttgcg gatatcaggg aagtgaaggt
    36241 agaagaattt aaaaaaaacc ctgttaccgg aagttatggt gcacttgtga caatgacaaa
    36301 taatgaaggg aaatctgttt attttgatta tagtttttgg aaggagagaa atgaacttgg
    36361 tggttttggc attgagaaca gagcggtaca aaagacagga gtcactataa aaagagtaaa
    36421 agtaattttt tctaacggac aggaggagtt agtatgacaa taaacgataa gcattacaat
    36481 gatattagtg agaaggttta ttggctagat cccaaatatc cccgctacaa tgagggatac
    36541 aaaaagaact ctgttaaaga atttgctggt atggaatttc aaatcctaca aatcaaagac
    36601 tcattggacg gcatgcaggc gatgtctgta gcgccaatca agaacggtaa agtggatctt
    36661 tctcaagtag tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg
    36721 gatgcaagaa gtatcatgaa tctctacggg aatagaccag gagcaagttt ttctgaaaaa
    36781 gcaacaacaa acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca
    36841 gcgattgcat ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc
    36901 ggccattcat taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat
    36961 gtcggcttca acgggccgga cccgtatgat ctcttatcac cagaagcaaa aaaatgggtg
    37021 gaagaaaatc ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt
    37081 ggtggtaatg gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg
    37141 cttgcttccc acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct
    37201 gaaaatgaaa acaatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg
    37261 ttttcctatc agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc
    37321 cagctgacag ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa
    37381 aagttatcgg caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga
    37441 atttttgagc tagagaaaaa atggcaagaa ggcttgcggc aagcacgtgt tgtagcccct
    37501 tcgttgactg aacaggaaat catagaagca ttagctagtg tgggcgccac aaaagaaaac
    37561 ttagtcgatt ctgcgaaaga agtctatctg ctacgaatca ataaggcaag acgtatgggg
    37621 gaggcctttg atacattggc gaaggagatc caagacaaaa tcaatgaaat cgtggcacga
    37681 gatcgagaat tagcacagca attcaattaa aggagaagta gatgaaacta gaagaattgg
    37741 aaagagatta tcgaagtgaa cgaagacaat tagaagctca agaagatgaa ttgatccact
    37801 ttaagaatca aggattacga ttgatggagc aagaggagca gcgactgcat catgtattaa
    37861 agaacttagc tttagataat gagcctctgt ttcaagcaag acgaagttat tcgaattatc
    37921 aggagcatta tcaacaagcc ctacaagtag aacgacgaaa actctatcag cagcaagatg
    37981 aactagaacg ttcctatcgt caaaaaatca gacaaataac tatggagaaa tcatcttatg
    38041 aatgaatttt taatgcaatt actaaaagaa ttagagaaaa atctacatcg atcaaatgta
    38101 cgttcagttg gtatggggat atctcagcaa atttgggcag aaaaacgatt gacagatcaa
    38161 aactaccaaa tgactgcaga agaagctgct caagaatttt ctaaccgaca tgtacacttg
    38221 aaaggatcag taaaagggca atttgcagaa aaactaactg aattattcca aagacaagaa
    38281 aagaaattaa aacagtttcg ttagtaaagt tatatagaaa aattttgtga aaat
//
LOCUS       DACEKG010000021        36939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEKG010000021 DACEKG010000000
VERSION     DACEKG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.021"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..523)
                     /locus_tag="IW664_000978"
     CDS             complement(95..523)
                     /locus_tag="IW664_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848357.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(551..1189)
                     /locus_tag="IW664_000979"
     CDS             complement(551..1189)
                     /locus_tag="IW664_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848358.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1193..3229)
                     /locus_tag="IW664_000980"
     CDS             complement(1193..3229)
                     /locus_tag="IW664_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0848359.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3355..4020
                     /locus_tag="IW664_000981"
     CDS             3355..4020
                     /locus_tag="IW664_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848360.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5955..9104)
                     /locus_tag="IW664_000982"
     CDS             complement(5955..9104)
                     /locus_tag="IW664_000982"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ0848361.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9117..10316)
                     /locus_tag="IW664_000983"
     CDS             complement(9117..10316)
                     /locus_tag="IW664_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0848362.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10306..11901)
                     /locus_tag="IW664_000984"
     CDS             complement(10306..11901)
                     /locus_tag="IW664_000984"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0848363.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12485..13192)
                     /locus_tag="IW664_000985"
     CDS             complement(12485..13192)
                     /locus_tag="IW664_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848364.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13257..14093)
                     /locus_tag="IW664_000986"
     CDS             complement(13257..14093)
                     /locus_tag="IW664_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0848365.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14111..15520)
                     /locus_tag="IW664_000987"
     CDS             complement(14111..15520)
                     /locus_tag="IW664_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0848366.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAGHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15534..17357)
                     /locus_tag="IW664_000988"
     CDS             complement(15534..17357)
                     /locus_tag="IW664_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0848367.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18200..18526)
                     /locus_tag="IW664_000989"
     CDS             complement(18200..18526)
                     /locus_tag="IW664_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848368.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18572..18934)
                     /locus_tag="IW664_000990"
     CDS             complement(18572..18934)
                     /locus_tag="IW664_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0848369.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18971..19186)
                     /locus_tag="IW664_000991"
     CDS             complement(18971..19186)
                     /locus_tag="IW664_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0848370.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19313..19648
                     /locus_tag="IW664_000992"
     CDS             19313..19648
                     /locus_tag="IW664_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0848371.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19724..21721)
                     /locus_tag="IW664_000993"
     CDS             complement(19724..21721)
                     /locus_tag="IW664_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848372.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21819..22094)
                     /locus_tag="IW664_000994"
     CDS             complement(21819..22094)
                     /locus_tag="IW664_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848373.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22326..23060
                     /locus_tag="IW664_000995"
     CDS             22326..23060
                     /locus_tag="IW664_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0848374.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23099..24241
                     /locus_tag="IW664_000996"
     CDS             23099..24241
                     /locus_tag="IW664_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0848375.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24336..25727)
                     /gene="sufB"
                     /locus_tag="IW664_000997"
     CDS             complement(24336..25727)
                     /gene="sufB"
                     /locus_tag="IW664_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0848376.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25732..26202)
                     /locus_tag="IW664_000998"
     CDS             complement(25732..26202)
                     /locus_tag="IW664_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0848377.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26189..27424)
                     /locus_tag="IW664_000999"
     CDS             complement(26189..27424)
                     /locus_tag="IW664_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0848378.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27421..28707)
                     /gene="sufD"
                     /locus_tag="IW664_001000"
     CDS             complement(27421..28707)
                     /gene="sufD"
                     /locus_tag="IW664_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0848379.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28720..29493)
                     /gene="sufC"
                     /locus_tag="IW664_001001"
     CDS             complement(28720..29493)
                     /gene="sufC"
                     /locus_tag="IW664_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0848380.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29690..30538)
                     /locus_tag="IW664_001002"
     CDS             complement(29690..30538)
                     /locus_tag="IW664_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848381.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30562..31248)
                     /locus_tag="IW664_001003"
     CDS             complement(30562..31248)
                     /locus_tag="IW664_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848382.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31241..32275)
                     /locus_tag="IW664_001004"
     CDS             complement(31241..32275)
                     /locus_tag="IW664_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0848383.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(32670..33008)
                     /locus_tag="IW664_001005"
     CDS             complement(32670..33008)
                     /locus_tag="IW664_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0848384.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33010..33366)
                     /locus_tag="IW664_001006"
     CDS             complement(33010..33366)
                     /locus_tag="IW664_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0848385.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33372..34559)
                     /locus_tag="IW664_001007"
     CDS             complement(33372..34559)
                     /locus_tag="IW664_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0848386.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            complement(34750..35595)
                     /locus_tag="IW664_001008"
     CDS             complement(34750..35595)
                     /locus_tag="IW664_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0848387.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35829..36794
                     /locus_tag="IW664_001009"
     CDS             35829..36794
                     /locus_tag="IW664_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0848388.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        10954 a         7583 c         6121 g        12281 t
ORIGIN      
        1 tacaactaaa aatgaatata ttaaaactac caaaaataac ttttaacgct gtttcattat
       61 atgttttttc tattttatat aaacttttgg atacttaata ccaaccggtt ggattgtacc
      121 aacatgagaa tgtgtcagtt ctatttcctg tcgcacttgc ataggcccat actccttttg
      181 ttgcatttgc tgatcgaata gagtttccaa ctttcgcttt tgcaccgtga cgattataga
      241 cacgactata gtggttagaa tgacctgttt ttctcgtaca agttgtatca ctataataaa
      301 cccatcttcc agagctatga ttaattgctg ttctagaaat ttcccggtct actggtaaat
      361 ttgacttaat aatattacct ttagcatcaa aatgaatctc tcctggcttt aaagatttgt
      421 aatagatttc accgtgaact gaatttgctt ccacttcaga tgacatagcg tttcctccaa
      481 caaaacctat ccctagtgca caaataataa ctgctttttt cataaaacga catcctttcc
      541 tttttgattt ttaaagcatg atcttcttgt ttcctatttt ggtcagatat ggatcatgcg
      601 tgactacgat aactaacgta ccttgggaag cttgttgcga taagatttca actactttat
      661 ccttatttct tttatccaat gacccagtcg gttcatcggc taaaatgatt ttgctattct
      721 tcaataagac tctcgcacag gcgattcttt gcttctcacc accagataat gtgtagatag
      781 gcgtacttag cttcttgttt atatttactt ttttcaatgc ctcatacttt gctgttcgct
      841 tatctgaaac atttaaattc gaaaacttca atccaatatc taaattaaaa tcgattgttt
      901 catattcaat caacccaaaa tcttgaaaaa gaaaagatat ggtatttctt tttatcaagc
      961 gcttctctct cccagttaat tgtttcgtct cttgatcgaa caaatagaat tgtcctgaac
     1021 tgaactgatc attcaatcct aatatattca ataacgtact tttacctgct ccactttctc
     1081 caacaacagt cacaagatcc ccagcatccg cagtgaaacg atccaaatga agaatcgttt
     1141 tgtttcgaat gatttttttc gcacctttta tcactatcaa cggctccaat tgttaacctc
     1201 cattcatcag caataactga cttttctttt ctgtagacaa aataaaaaca actgaaagaa
     1261 taaaatcaat gaagctaaag aaaaaaatat taaaaagata tttaggctct ctttctataa
     1321 gaaaaaggaa gattacactt gtcgttcccc atttgatgag gttcatcatg aagaaatctt
     1381 tatacgtttc aaagagcgaa taaccacttg aacgaaagat agcgattttc cgacaattgt
     1441 agacaaaata tatgcataac atgacatagc tcaacaagaa gaaagaaaag aaagtgagta
     1501 aaaatatctg tatcaagtaa tgaaatgatc tgcttaacac tttgacagct atctcttcag
     1561 tctgagtata cggaaatgat aactgcaagt tatcacgtaa ctgactttct tcaatcagct
     1621 caaataacct ttctttcgac aaagagccta tcttgatctt taacggtgga tagatttctc
     1681 cgctgagaat atttctttcc aaatacatac tgtttttcaa tgtcagtatt tcaacaaccg
     1741 gataatgttc gatccagggg atagaaggaa taaatgtata aatgggttga ttgtttttta
     1801 taaatagaaa tttcggttct ttttcactga tttcttgata atattctgtt atttgcgcca
     1861 ggtcattttt taaatctgta tgtctatatc gttcagggat caatataaca gggtgcgttt
     1921 ctttttcggt tatttggatc aaccttccag agacatcaat caaaggaaat ttcttaagat
     1981 aattcagatt tatttttatc cctctgccaa actgttcatt ttcttcattc aagtaatctg
     2041 atacattgac taatatactt ccgtttttat tcaaagtttc atataattcc tgatctgctg
     2101 ctgatgcaaa acgattggaa taaataaaat ccacgtggtt tttcccgatg gttactggat
     2161 aaaatacatg atagtcctca ccagcgattt cttttggtgg ttcatgggaa acagctaact
     2221 gtgaagaaga aaaaacgata gttgctaatt cgacattggc aacggtcaaa agaagagaaa
     2281 acaacaatcc aaatgtaaaa agaatgtatt ggatatagtt gaacgcttct cttaacgaag
     2341 cgattttttc aactaataaa agagaaatca aagagatacc atatattccg ctgaacaaga
     2401 aaacgtacat ctggaacaaa taaagaaatt gctccttata atacataaaa ataaaaccac
     2461 caatgattcc tgcaattatg aatgtaataa tgattttctt tttccccaat aaacaccaca
     2521 atacttgatg taatgaaaag tcattattca acaataaact gatttcttta ttcatcaata
     2581 agaaaaagga tgataacaaa aggaataaaa gaatcaatcc aatagttaaa tacactcgat
     2641 aattatatac gtctgtattt gaaataaccg aatacgaata gctattttca aagtcttcaa
     2701 aaatattagt ggcataatca gtgtgaaatg cttgattgaa tcgatcagtg aatagtgaaa
     2761 tgacccgttc ataggcttct ttttctctcg tcatcaagaa aaattcacct tcaaaatctt
     2821 ttattttgat cgattcttca atggacgtta cgcgaaagga ggtattagaa tagggtacct
     2881 tctcgtgaga agatttgaca tccccttgat aatacaatgt catttcaagc tttggctgta
     2941 tcttgaagtt ccaagatgta tctacttcat tggattcatt tactgttttt ttcatataag
     3001 aaagctcagt ttctgatgag acattttcaa ataccatgat tagctgctca tattcttctt
     3061 gcccaataaa atgttgtggc aatcgaatcg gagtataagt atctgtttga tggaaaaatt
     3121 catttgtccg atagagtacc tgtttattta tactaaccaa ggcaaaacca ctcatgatac
     3181 cggtcactat gattgtgagt aaaactgtga actttttttt gattatcacc actctccctt
     3241 acttaaatga ttctttgata aaatcatacc gtttttcatc aaaaaaacgt atctatcaag
     3301 tgtataggtc atgtagaata aatctatata ctgtagaatc gaaaggagaa gaagatgaac
     3361 accggagaaa aaatacgaac ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc
     3421 gaaaaaattg gtgtttccga taaaacgatt tctagttggg aaaattctcg tacaatgcca
     3481 gactttgaaa tgctgtcact tttacatcat actttaggtc tgccgattga ttttccttct
     3541 attgaatcag ctaccgaatc aatagaacca acattgccag atgaacctct tgttaacaag
     3601 aaatcttatt catcaaaaca acccttctca ctcaaaaaga cactatcttt ctttctgctc
     3661 gtcttttgtt tggtctatct ttttcttatc aattatcaat acaaaaatga aacgatcgat
     3721 cgattcaatc cttttttgaa accacaaatc ggctatacca cactccctgt aggatacgtg
     3781 gacaattcca tcgaacgttg ggtatgtaat gatatctttg gccatggtac atggctgtct
     3841 tttcacgctg gagaggtaga aaaagagaaa aaccatgctg taatcaaaca caaaggaaaa
     3901 cacgtcatga acgttcgcct gctcacttgg gaagccattc cctcagatta ccaagaaatc
     3961 ctcagccaag actatgacga cgccttaaaa agaagccctc aggagccaga taggcattaa
     4021 gtaggagatg aacgtagaga tagaaaaaat caatgcacat gatcttatcc aaaaaacagg
     4081 ggtacaaatt aattgaaaaa gccttgagat gaaagttcct aaataaaaaa gttcagaatg
     4141 ataggtattt ctggcttata gtataatatt tacgccacag cctcccaatg attctttttg
     4201 gaatcataat caaggatgga cattcacaaa aatattttgc tcattcgatc cgaatagatt
     4261 aacgaccaat ctagtaagta atatagattg gtacgtagtc aagctgcaca taatttagat
     4321 caattttagt tcttttttat atttctatga tctgctcatt tacttttata tgatggctta
     4381 cagtaacata aataaatatg ataatcgctg gtagtttatc caagagtact caataaaata
     4441 caaaatggat tgttcaagcg cctatcataa tgataaaatg aaacaaataa acgatacaag
     4501 gaggaagaga ctttgagtaa tggcagaaca attttttcat tgtaaaaatt gttcagttgt
     4561 taatctatgg gatttcggta gcatgggcat ttttttatag aggaatcaca ccaacctggc
     4621 cgttttatct cttgttagtg ggaatattag taggaaattt tgcccctctt cactatcgta
     4681 cgaacttttt tccaactgat tctggccttt ttcttaaaac aaagacgcga aaattagaaa
     4741 acattattga aatgacaatt acctgtataa tcattttctt agttatattc ctaaacgtct
     4801 tctaataaga atctcttaaa attagcttgt tacagttagt tacctagttt atataacaca
     4861 gttatcttat cttccttatc aacatctttt gccaactctt tgtcttaaat tcgaaacata
     4921 taagcagagc tacgaacacc aagactgttt cggctctgtt ttttgcatca aattcactct
     4981 tatatagctg ataatctcgg ctctttaaaa gaaattattt tcgcttcatt ttattataaa
     5041 aaggctagga accttgatgt tatcaggttc ctagccttta tttaagtatt accccacaga
     5101 cccttccata tagattttta aattacacac tagtttaaac taaaacaaac aaggaaaaac
     5161 ttaccattta atcaaaaaat tagctcatat gaatatatta gaatgtaaat tagactggtc
     5221 aaaatctggt caaaaaagag gacgaataca ggtagtcctc ttttttgtta ttttgatatt
     5281 ctaaatcatt ttttattgga ttattccctt gttcgatata gacccaaatt gatctatcaa
     5341 taattatcaa actgagtcat atagagacaa aaatttcaaa aatgacctgt taaatggtca
     5401 tcgaatatca tttaaaatca atacgacgga gtcagacacg gagtcaaaac aagcccgctg
     5461 agagtaaaaa atatacagtc ccataggtaa agtgccacag gactgtatat ttttttattt
     5521 gagattacct gaaatgattc catttaattt ttatctcatt ttttcctact agttagtatc
     5581 aaatactctc ctattttctc aataaactat tttgggttac atggtatctg aaacagctaa
     5641 aaccttgtca ttatagcaaa aaatcacttt atctccgttt catttcagat acatttcact
     5701 taagcggatt ccaaaaaaac aaaagaaaaa ggaaccccta gttagcctat gctttttatt
     5761 ggttcctctc aaaacacggt catataacaa cggggtgggg taaatgaatg aaaaaggtgg
     5821 aggaaacatt attcaatagc tgtcttaaat acttcgttat aaaatgtgaa caaataaaag
     5881 acatctatcc ctattttcta cacttccact tgactaccat ctttgaatcg tacaattttc
     5941 ttaactcttc tcgtttacct caattcattc tcaagtggaa tcaactcatc atctaacatt
     6001 ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gcttcgcttc tggatgtttg
     6061 gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg aattggatct
     6121 tcacctggta gatattgcag attagaagca tgtaattctt tctcagaaac aaaccactca
     6181 ttggaaaaga catgaatcaa atcatcactc gcttgtgtaa aatagcgacg tttaatgaca
     6241 aagatatctt cattactatc taaagtcccc acatcaaatg catttaaaat ttgtcggtaa
     6301 acttctttga ctccttctgt tttattcaat ttagccaaag ctttttcaat ttcttctcgt
     6361 gcatctgaac tattcgcagc ataagttcct agtaattgat caatataagt tgaatcaata
     6421 tcatacaact tagtggtatt ttcactataa aattcgatcg ttgaaaaatc ttctccttct
     6481 ttttctggtt cttcctctat taatgaaccc ttcacggtct cgtacacgcc aatttgttct
     6541 tccaaaagtt gaatttgatt gttcaaagtt ggagatttca ccatgtcatc attgtaatcg
     6601 tcataagtca ctaacgattc ataagcacga ttcaattctt gaaatgcttt gacgtactcg
     6661 atacggattt caagtggcgt atgttcatca atggattcca tggatgaaac ctcttttaac
     6721 ttaccatatg cttgtttgaa tctttttttg gattcctgat aacttggata aatcaaacct
     6781 gattcttctt ttgcttttga atacagtttt gtcgcatcct cgacattctt ctccattgtc
     6841 gctggcttac gaaacgtcac aatcaatcct tttgtcttat caggatacgt acgattcgta
     6901 cgtgaaaaag cttggatcaa tcctgcatac tctagattac gatccacaaa tagggtttga
     6961 atcgtagggg cgtcaaaacc tgttaataga cgatccacta caatcgctaa atcgatttgt
     7021 ttcccaaatt gtttgaattc cgctcttttt cgtgccaaac gattattgat gtctccgtta
     7081 taacgctcaa tgtctgctaa tgaccaggaa gtatcataat actgattata ttcctcaata
     7141 attttttgca tttcttcttg ttgggcactt gaatctcggc tattttcttc taatgaatag
     7201 gtaatagcga ttcgtggaaa atcaggatcc tccatcgttc gtccttcacg aatgagctga
     7261 ttgggaaact cttttgttaa ccaatcaggc tctttcgtca tctctttgat ggcccgataa
     7321 tactttttgg ccatgtcaat ggaactcgtg gttaagattg cagatttcgt tggcctgcca
     7381 tttcgaaaat caaattttgt ataggcatta tctggacgga acattttatg aatgactttt
     7441 tggatatgtt catctgcttc aaaaacagct gggtccaaat aactttcttt tttgacaggt
     7501 tccatttgat caatcatacg attgatctgt tctgatgaat actctgcata tgtttctact
     7561 tcttttaatt tccgatagat tttattctct agtgaagtcg gctccacggt gttttcatgc
     7621 tctacttgaa atcctaagac agagccatct tctagcgcat tcttaatggt ataggtatgc
     7681 aagacctctc catactgatc atgggttgta cgtgccaatt gccctttggc ttgtttccga
     7741 ttctcaggaa aaatcggtgt acctgtaaag ccaaaccaag tggatttcgg aaacattttt
     7801 ttgatttctt ccatattttc agcacttaac gctcgatgac actcatctac tacaaacacg
     7861 atatgttggc ccattaattt ttggaagcga ttggttcctt ttttctcttc ttgtttcttt
     7921 gcatatttca acgccgcatc tagtttttga cgggttgtaa tgattacaac atttgcattg
     7981 gcatctgcca acagggtttc acttaattct ttggcacttc ccgttccaac gatcaagcta
     8041 ttcgctttgg cgttcccgga agaaatccca gtattaaatt ccgaagcaaa ttttgtaaac
     8101 tctgtggtgg tttgattatc taaatctttg cggtccacta acatgatcgt tcgatcgatc
     8161 cccgattttc gtgctaaaag cttagtagat acaaaactag tcaacgtctt tcctgaacca
     8221 gtcgcatgcc acacataccc tgattgatgt ttatttgcgg caataaacaa ggcttgaatc
     8281 gcatgtactt gataaggatg taacaccatg agggttttat tatcttgatc ttcactgacg
     8341 attgtataat ccgcaatcaa acgatgagca tccgggatat ttaacacttg tttacaaaac
     8401 tcatataagt tttctacttt ttcattgtct ttcgttcgcc aactaaataa aaacttaggg
     8461 tgcaagtctt tcggtaaggc atttgcaaaa taccgagtgg tttgttcatt ggaaaccaca
     8521 aacaactgta aggtcgaaaa gatattgttc cgaaacatac cttcttctgc atattttttg
     8581 atttggttaa atgcctgata aacaccatct tttgcagtta cttgtttcag ctcgatttgg
     8641 acaatcggta atccattgat taataaggtg acgtcaaaac gtcgatcccg tgcttctatg
     8701 ttacttccac gttttgcaat ttgatggacc acttcataag tagagattcc tccaccaatg
     8761 tcttgattgg aataaaggat aagtgagaca gaacccaatt gcgaatcttc ccgttcaatc
     8821 gtgatccgag ccatcccatt ttcacctttt aaccatttcg cagcatcaaa tggggttttc
     8881 gttcgcagca gtaattccgt tttgatcgtt tcaaattctt tatcggtcaa aggaaaatca
     8941 ttcagttctg cttgattatt tgagataatt ttttggcgta aattgaccca aaggtcttct
     9001 tctgatttta gatccggacg atacgtccac tgattatgcc cttctccaag tacacgaatc
     9061 aactgttctt cgacttctgc ttcctcacgg tgactaattt tcattcttct tccctcctag
     9121 acaaacatct tttgtaagaa gctttttttc gtttctttta gtaaatctaa cttacgttga
     9181 tgaagagcga tcgtatcatc tagctgtttg aagaaagtac cgattttttg ttgttcatcc
     9241 tttacaggaa ccttaacact gatattctca atatcagaac tgttataaga aggcaatgcc
     9301 cctacttggg agtatcttgg caaatttata gtttcaaaca atattttacc aaaatagata
     9361 tcccaagtat tattaaataa ataagccatg gtattgttat ctattaagaa agggcttgtg
     9421 accaaacgtt ttctattaag cattattgct gcgccaactt tagcaaatat aatcgctggc
     9481 acatctttta ttggcttcca ttttttacgc tcaatttgct cattagatac ataatgattt
     9541 gcatttctca tttcatactc attaccaatg ttattcatgt cggaaacttt aaaaaatgga
     9601 gtaccttcag aaccaccttg ctcggcatca ggaaatccaa ttcccgattg agttttacct
     9661 aattctccca acttacgctc ttcccaatct tccgtaaaac caggaaaccg gatttctggc
     9721 acttttgctc cgttttcagg gaacattttt tgtaagaatc cttttttcgt ttccttcagc
     9781 aaatctaact tacgttgatg aagagcgata gtgtcgtcga gttttttgaa gaatatgccg
     9841 attttttgtt gttcttttaa atcattggga atatagaata ctgtatctga aacttttttc
     9901 cagtctgaac gaggcatttt tgttccagat gtatcatttg cagccttttg atatctatca
     9961 gcttgaataa gagaataaat aaagtccgag tcgctcttct tagctctaaa aacccagaaa
    10021 tccccaatcg ctacgcctgt aaaagttgcc ttgagccaat tttttaaata tggacgtaac
    10081 tttccatata gaatatcatt tggtaaaaat tcaatccctt ttctattatc aaatttctgg
    10141 ctcacatctt tattcagtct accttctcct gccacgatat cttcaaattc aacttttgga
    10201 agtctactgg agtttgtact tttattaagt ctaacaacta aatcaattaa cttacgctct
    10261 tcccaatctt ccgtaaatcc tgggaaacgg atttctggtc gtgtatcatt gctcatgttt
    10321 gaacacctcc agcgcacctt gtaaccacat ttcgttttca ggtgttgttt gcagggaaga
    10381 gatagcttct aaaagttctt tttctagcac ttgctgttct tgtcgtatct tcttgatgtc
    10441 atttccgaca tgaaccatat caattggttc ttcttcttcg aaagtatcta catagcgtgg
    10501 gatatttaag ttatagtcat tttctttgat ttcatcaaag gttgccaaat gagcgtattt
    10561 ctcgacatct tttctttcag cgtaggtttc gaggattttt tgaatgtttt cttccgatag
    10621 tttattttga tttttccctt taacaaattc acgactagca tcaatgaata atacatctcg
    10681 agtttgacga ttcttcttca acacaatcac cgtcgttgga atcgatgtcc caaagaacaa
    10741 gttcgcaggc ataccgatca cagcataaat actgccatct tctagtaatt tttgacggat
    10801 gaccccttct gcagcgccac ggaataaaac tccgtgtggt aagacgatgg ccatggtacc
    10861 tgtttccttc aaatgataga acccatgaag aagaaacgca aaatctgctt tggattttgg
    10921 cgctaatttc ccataacgat taaaccgaga atcatctaag aaagtcgtat ctgctgacca
    10981 attagccgaa taaggtgggt tcattactac tgcatcaaat gtgtaaggtt catctgttgg
    11041 ccagtcctta ttcaacgtat ccccattgcg gagattcatt tcttcggcgt cgacaccatg
    11101 taaaatcaag ttcatttttg ctagattgta tgtcgttgta tttagttctt gcccatgata
    11161 tttaacgtta tctgggtgtg ttaagtagtt acgtacgttt aacatcaatg aacctgaacc
    11221 catcgttgga tcaaaaacac taaagaatcg tcgttccttt tgatcgagcg ttacgatttg
    11281 cgccatcata tcagaaacca tatgaggtgt gtaaaattct ccggctttct tccctgcttc
    11341 tgaagcaaat tgactgatca gaaactcata cgcatccccg atcacgtccc catcgtgttc
    11401 taaaacatct acatcattca atttcttgat gacttcggta atcgtcacat ttcgttgttg
    11461 ctcatctgtt cctagttttt ttgattggag atccacatca tcaaataacc cattaaattg
    11521 attgtaggta gatgccaatt gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc
    11581 ttgttttgct tgatccgcta agacgttgaa aagatactct ggtgcaattg catatcccaa
    11641 aatatcaacg attgtggcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg
    11701 atacaacatt gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
    11761 aaccacttgt tccaacaatc gatcggataa atatttatag aaaatcagtc ccaataaata
    11821 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataattt
    11881 ttgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
    11941 ccctcttatt ttagcatacc cccttttata aaaagcaaaa aaaaaagaaa gaaacttatt
    12001 cttgctaata aactacgcga aaggagtcta acgttgtttg atataactta gactgatcgt
    12061 taacttattt tttatatttc tacatagtta tctttagaac catttcagat acatatgaaa
    12121 caattacata tgattttttc ataaaaaaac agtactgtct agtagtataa aatatacaaa
    12181 tttcttaaaa attttttcac taaaatcact acctgaaaca gtctaaaaca cttatatatc
    12241 aacgtttaat gaaactatct ccgtttcatt tcagatacaa ataaatatag tcgggctcca
    12301 aatccgaaaa aaaatacccc cagtagctta tcctaagcta ctggagatgt ataagtacgg
    12361 tcatataaca acggggtagg gtaaataaat gaaaaagatg gaagaatcat tattcaataa
    12421 cttttttaaa tacttcgttg taatcagtta cctgtccgtt cttaaatacc acagaataat
    12481 ctttttaccc attttcaagt aaccgaagtg gaatgtcttt accaacttgc tctgtcactg
    12541 cattactgat acctgttaga ttagctaata aatctgataa agattgagca tctgtagaaa
    12601 tctctgtatc agtatttatc gtgattcctt tatcttcatt tacaagcttc caagtaggtg
    12661 gtatagctgt gtatgtttcg atgaatgaat caattctttc atctatcgtc ttcccagtat
    12721 ctacttctgt ctcattactc ttagatgaat cgattctttc atccatcgtc tgctccgtaa
    12781 ctgcttctgt ctcattattc tcaccggaaa ctctcttcac acccaaactt atatattgtc
    12841 catctttagt gtgcatagtt aaaattccca cgcttttact aataaattca ctataaccag
    12901 ttctttcaat agagacttca atgtatttca cactatcata ctcaaaagaa aaatagacat
    12961 gtaaaagcat tttgttgttt acatctacat aataatcctt tgactctact tgagaccttc
    13021 ctatacggaa tacagcttta ctgtctgata tctctatacc agcatcctta ttattaatct
    13081 gccaatctcc aagtagctct tctggcaagc tagtagtttc ttgtgaacac tttaccactt
    13141 cactggtaga ggtcgaaaca ttcacctccg cactgttaga agaattttcc aaggaatcag
    13201 gagccttagc acaaccaaca agtctttata tattgtgcag ccgatatttt cttcattcag
    13261 tacacccttg aaatatgcag tgttagataa agcagttcgt cctgatttgc tgaggcaccc
    13321 atctcttctt gaacgtactc cttgattttc aaggccgccc gatactcttt cggaaaacgc
    13381 tgcttcactg aagccagcaa aaaatcatcc ttctccggca actcttcctt cttaatcatt
    13441 cgggcaataa aaaactgtaa atgcgtcaca aatcttaagt aaagcatcga atctttatca
    13501 accttttttt cgggaaacag atatcctagc atagccaaga tatcctttag tgctcttacc
    13561 gagagcatgg tttcttccat atttgcttgt tcactttggg catttacaat gtgaaacgca
    13621 atgtttccag cctcctcttc tggcaactga acattcattt ttttgttgat caattggata
    13681 gcatagcagc cgatctcaaa ttcttttggg taatactttt gaatttcaat cagcaggcga
    13741 ttttgaaaaa caatgccttt ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag
    13801 gtaaaaaata gacttttatt cagcttaaag tcaaacttgg cgttggcata atcgataatt
    13861 cgcttgacgc aaataattag atcatccgga gcagcctcaa acagtttaat gtaatccttt
    13921 tttagagcat cagaaagtga atctaattta aatacttttt ggattttgtc agccggaacc
    13981 aaatcgcctt tctttagttg aaatccaatc cctttcccca tcaaaattac ttctttgcct
    14041 tgcttatcta agcaaagaat taaactgttg tttaacgttt taattactct cattagattc
    14101 accgttctct ctaagctaag ccatttcttg cgattacttt ttgataccaa aagaagcttt
    14161 ctttttttat tcgttttaat tctttaagat tctcatcggt tcggtcaaca tatataaaac
    14221 cataacgctt attgaagcct tgatgggtac taacaacatc aatcgctgac caaggtaaat
    14281 aaccaaaaac atctacacca tctgttaaag ctgcttgaat agccagaata tgtttggcta
    14341 gataatcaat tcgatcctgg tcattaattc gtctatcttc ctcaactatg tcgggtgccc
    14401 catagccatt ttcagtaatt aaaataggta aatcatagcg atcgtacaat ttcctcaatg
    14461 ttaatcgtag tccagtaggg tcaactaccc agccatatgg cgttttatct acataaggat
    14521 tttcagctgc ccggtagaca ccttcttctc ccaacatgat ctgttgatcc cccgctctag
    14581 cggaaacatc tgatccgtca tttttgctgg cagcgatggt ggcagttgaa taataattaa
    14641 tcgcaataaa atcgggttta ccggaggata aaattgcctg atcctctgcc tcaattttag
    14701 gatataatcc acgatcattc aaatagtttt caaacaaata attgtatttt ccccgaactg
    14761 ctacatctaa gaaagaccag cctctcaatg tttcccaatt atggcctgca attgcatctt
    14821 ctggactgga ggtagcttga tacatagccg ttaaattgat tgctggaccg atttttcctt
    14881 taagattcaa ctgatgatac aagtgaatta ctctagcttg agctaataaa acatggtgat
    14941 taatttgaaa gagttctttt tttgaaggca gctctccact tggtggcaaa ccaatcgctc
    15001 caggatgaag gatcatcgtg ttttgctcat taattgtcag ccaatagttc accttgtcac
    15061 catactcagt aagcaatacc tgtacatagt ccacaaaata atcgatagtt gctcgattta
    15121 accaaccgcc ctgctcctct agaaaataag gtaaatcgaa atggtacatg gtgacaatcg
    15181 gttcgattcc ctgattcagg cattcatcaa ttacttgatg ataaaaagaa attccctttt
    15241 gattggtttt attattttca atatccataa ttcgcgtcca agcgatggaa aagcgaaaag
    15301 cttttaagcc taattcttta aacagctgta tatcttcttt atagtgatgg taaaaatccg
    15361 aggcaacttc aaaattagct accccttctg tatgctgata cttgtcaata atagacggtt
    15421 gtttcccatc ctcctgatag gcaccctctg cctggtaggc actaatggag gcaccccata
    15481 aaaacttctc tggaaatgct gaaagctgtt tgtattgcac aaaaatcctc tccttaacta
    15541 atttgcatca atggttcgtg ttcctttaag cttccgcttg cttcactaat tcctgcgtaa
    15601 ttttcactgt tggtcactac cacaatcact gtatcatcgt agccagcgtt tctaatggca
    15661 gcgaaatcgg cgttgattaa taattgtcct gctgacacct gatctcctgc ttttacaaat
    15721 acttcaaagg gttccccttt taaattcacg gtgtcgatcc ccacatggat caataattca
    15781 ataccttctt tagaacgtag accaatcgca tgtttagatt ctaaaatagt aatgacttct
    15841 ccctcaacag gtgaataaat gctgttttct tctggatgaa tggctacgcc ctttcccaat
    15901 agtcccttag aaaagacagc atcgttcact tcagaaatag gacacacggt tccttttacc
    15961 ggattttcaa caatgactgc ttcggctatt gccgtttgct ctgattcatc agaagtatcg
    16021 tcaaatccaa ttagataagc aatgataaag gttgcaacaa aggcgattac acacgtaata
    16081 attgcgtgaa caatattcat tggattttct ccaataaagg ctggtaaggc tgctaagcca
    16141 ggggaaacaa aggcatatct gactaaaccg ctcaagccag catacaaacc agcaattgca
    16201 ccaccaatca tggaagcaac taatggtttt ttcagcttca aggttactcc atagagtgaa
    16261 ggctcagtaa ttcccattaa agcagtgatc ccagtggaca cagctagctg cttcaactct
    16321 ttgtttttag ttttcgcggc tacagcaaag gtagcacctg attgagcaat attagaagcc
    16381 agcattcctg gcccattgat catttcagaa cccgtactgc tcaattgcat agtagcgatt
    16441 ggcgtcattg cccaagctgt acccgtaacc accaaaaatg gctgcaaacc acccatcaaa
    16501 aatggaatta accaagctgc tttgccgtta atccattcag cccccattgc tataagatcg
    16561 ttcagcaacg ttccaaacgg tccaatgaca accaacgcta aaggccccat aattaaaaat
    16621 accagcatcg gcttagtaaa gaatttaata aacgaaggcg aaattcgatc cgcaaagggc
    16681 tctacatagg acatgatcca aacagtaaag ataatcggta aaactgatcc cgcataatct
    16741 gcagctaata ccggaattcc tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa
    16801 atatttggat gaagcaaaat gccagcaaca gtaatcgcca aaattgggtt gacatcaaac
    16861 tttatggctg ccccataggc taatagtact ggcataaaat aaaaggctgc atcagcaata
    16921 gtattcaaaa tgatgtaagt ttgactactg tctgatagta caccagtcaa agtcaagacg
    16981 gccaaaatgg cttttaccat cccggcacct gttatagctg gaatcatcgg tgtaaaggtg
    17041 gtagaaatga cactaatcaa tcttgaaata atactttctt gattgtctga agaggggccc
    17101 tttttaattg aagttcccac ttccttttga atttccttga aggctgtttg gacttcattg
    17161 ccaatcacca cttggtattg tccaccgcta tccaccacac taattacacc aagtgttttt
    17221 tctaaggctt tcgtatcggc tttcctttta tctttgagtt caaaacgcag tctggtggca
    17281 caatgggtta agctgaccac attttctttt cccccaacct gttgcaatac atttcttcct
    17341 acttctctgt aatccattat tctctctcct taaagcagta aaaaagacca agtcaaacca
    17401 cgccccaatc aacattgaag tgttagtttg tcttggtctt gcctgcataa tcagtaacat
    17461 gccaataacg ttattttgct gtaatccaat aatagcacat tatgatagcg caatcaacag
    17521 ggaatttaat tttttatctt ttcgtttgac cgtatagggt aatataacag ttagatcaca
    17581 tcaactaggg tatatctgcg tatacttact gctaaaataa gtatatctaa tattaactat
    17641 accagtaaaa ccattcctac ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa
    17701 aatgcatata acggatcaca caacttcaac aattctgctc ttgggaaaaa ttgatgaatt
    17761 tggtgagtaa aggatggaat aaacagcatt ccaacaatat aggcaacaat agtgatactt
    17821 gtttttttgg taaaaatcag taagcaatag ccaatttcaa tcattccgag atataaaggt
    17881 atcaatagaa tgatttgggc tagtattctt aaaaattaac tagcaaatgt ttaaactgat
    17941 ttatttctaa tctacatgta gtaagtattt caattccacc aattatcaat aaaaatacca
    18001 aacatatcca taataatttt ttctttttca tttcttttat attgttataa cagtgaataa
    18061 cataaaaatt cccgacaatc attgtaatat ttttttgtca cctgtattag tatttaattt
    18121 ataggaaact ataaaaactc cctagtgaat ttacaccttt gggagttcct gtttggacgg
    18181 tatgcataat acatttaaat caatttaaca ttaacacctc agcatatggt atctccacat
    18241 atatacggtc tttctttgga tagtactcaa tatttatgtt gatagcactt ccattgtcta
    18301 atatagcttt agcccggtat gtgttatctc cattttcatg agtaatcgtc acatcatcac
    18361 atgatcgatt taagttgtac tgttcttgta aaatctgtgt taccaatggt tttgatgact
    18421 tctctaatcc agatgtacct gtcatttttg aaaaaataat acctataata gccaagacaa
    18481 caatacctat aatgacaaac ttcttgttca tatttacttt attcaacttt ttatctccta
    18541 tctccaaaat aatttgttct tatccctctt atcagtacat tgacatccct aaaagtacac
    18601 caaagattgc tccagctacc cctgctatca tcaaaatcgt acccattttc tcatcttttt
    18661 ctcgatataa gtagcccata ataacagcaa cagcaccaaa tacaacagga ataaatgcta
    18721 acgatactac catagaaatc caaccaataa tcaaatacag cttccctgtc tcttgtccat
    18781 gtacgatttg ttccttcatt tcattaattg tctgctcagt acttttcttt actgtgtctg
    18841 ttccggttgt ctcatctgtt tcttgtagtg ctccacaaaa ctggcaatac ttactttcat
    18901 ctgttatttc tttgccacat tttctacaat acatgtcttc tcctcctaaa atcattcaat
    18961 tgcttattag ttaactgttt actttattaa atacatcaga aaacagctca ttcattggta
    19021 cacttaatat ctcagaaagc atcttcattt gatagggttt aggaatcgtt ctccctgttt
    19081 gccacgaact tacacattgc tgtgtaacat tcattttttt tgctaactcc gtttgagaaa
    19141 gtcctttttc ttttctgtat cggctgagat tacttacttt tttcatgatt ctcccctcct
    19201 gttttattat atacaacaat atgatgtatt tcaactcata aaatataaaa aacttcaaat
    19261 accattacaa atacaacaac ttgttgtact atactgagat gggggtgtat taatgaaaca
    19321 gaaacaatat gcaaaggtca ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga
    19381 attagctgaa aaaatcttct ctacacaaca ggcagtagca agatgggaaa attctgtaac
    19441 agagcctaac ctcgatagct taacagctct ttctagagcc ctaggaaccc ctgtaagcca
    19501 ttttctagac aatgttgtcg tagattatga ggaagagttt ttggctctct atcgttctct
    19561 tagcactgaa gatgcagttc gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa
    19621 tgaacgcaat cagctattga aagattgaaa aataaataac ccaccgaact ttaacggtgg
    19681 gtgtattgag atgaaataag attaagagct atggataacc tgtctagttt aatacaattg
    19741 ggtcttttct cttccttact ctccgtccat catctcttgt tacacttgta aattgataca
    19801 gaggaactat cccagctcct gataatatca attcttttat tgtttcatct atccctgccc
    19861 ctttcgctaa atatattaaa aaactactac ttgctttcac attgtatagt ttcttaatat
    19921 ccttagaagc tcgttcaaga gtgaatcctt gtttaacaaa gtagcctctg ttttttatta
    19981 aagcattttt cacgctgtta tccccaacat tacgtaggcg ttcacaaaca gtgttaataa
    20041 gttctttctc tgtcattaga tatgtccctt gccacgataa gccctgtaaa acttcatgtg
    20101 cttcatctaa aatcaattgt ttttccttgc tgagttctga tcgggaattt tgaggaaata
    20161 ctcttttagc ctctttttcg ttaaaacata tcttaaccca ttcataagaa aaatattgat
    20221 atgtaaaatg aattttttcc agaagtggaa ggatatcttc attcaatttt tgaatcacag
    20281 aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgatta aagtttccta
    20341 acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga acatcttcat
    20401 catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta ttacatttat
    20461 ctatgtcata agttttacca tagtaagaac tcattttttt agctaattgt tcccccgaca
    20521 aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa agcaagacat
    20581 catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt ttgtctttca
    20641 ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga aaatgtgtag
    20701 gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg tctttcttta
    20761 aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg gaatgacaat
    20821 aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta tgatcgttgt
    20881 gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca atggttaaca
    20941 atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag tctttaaacc
    21001 actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg gtttcattga
    21061 ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc atggtaaaat
    21121 caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc actgcatctg
    21181 acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca cacccgggga
    21241 tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta attgcttctt
    21301 ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata cttttttgta
    21361 tcgcaaacgc agaattatga actaaattga tagataaaac cttttctatg cttaagtatc
    21421 tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca gtgttatcga
    21481 catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct ttattctttg
    21541 tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca tctcttgtaa
    21601 cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt tttgtaactg
    21661 gcttctctaa taatttttct tgatctacat aaattggaat attaatcatt gatgtttcca
    21721 tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata ataaaaacaa
    21781 actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata ccgttccatg
    21841 aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat aacaggtaac
    21901 ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata ttcacttgcc
    21961 tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag cataggcttt
    22021 agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat gctatcaatg
    22081 atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt tctaagaatt
    22141 atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta atcaaacaaa
    22201 aaatgattac cagttcatcg acatacacat aatacctcga taaaaaaggg gtgtcaacag
    22261 ctttgtaaaa tttatttaca acaccccttt tttgaggtat actaatttta ggaggtgtgg
    22321 tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata ctcggaacaa
    22381 aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt cttatgatta
    22441 acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga acctatttac
    22501 caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata gaggagttag
    22561 tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat agcattgtac
    22621 tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa cagttcccac
    22681 cagtacactc taatttagat acatggctgg atagatattc caaattagaa ccagagatgc
    22741 aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt agtcttttca
    22801 atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt gacaataaca
    22861 ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat gaatggctac
    22921 ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt actgataata
    22981 taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag aaaaaaatga
    23041 agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag atgatttaat
    23101 ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca
    23161 cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag gttttagtag
    23221 caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat acggtgttaa
    23281 aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa ctcatcagcg
    23341 taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata atcaaatact
    23401 tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc aaaaagtggt
    23461 agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga ctgcacaaat
    23521 tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa aaacacttct
    23581 tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg agcttaataa
    23641 tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct tcagattatt
    23701 ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag acatagacac
    23761 gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg gtaaagttat
    23821 aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata ctgagactct
    23881 ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac ttggatacaa
    23941 tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat acattccaaa
    24001 cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc gtattacacc
    24061 tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat ctgtcaaggt
    24121 agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct atgcacatat
    24181 tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg ctcatcagta
    24241 aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa agctagaacc
    24301 cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc ttccatctca
    24361 taactgatca atcgattgag ttcgactgca tattccattg gaagttcttt ggtgaatggt
    24421 tcaacaaagc ccatgacgat catttcagtt gcttcagatt cggtcaggcc tcggctcatc
    24481 agatagtaga gttgttcttc tgagattttt gagactttgg cttcgtgttc taaagagact
    24541 tggctgttgt ggatctcatt gaatgggatc gtatcggatt tcgaccattc gtccatgatg
    24601 atcgtgtcac attcgatatg agagatcgaa cccgcactgt ttttggcaaa ggttacttgt
    24661 cctcgatagt ttacttctcc gccgtctttt gaaattgatt tagagacgat agagctagat
    24721 gtatttggtg cattgtggat catttttgca cccgtatctt ggatttggtt tgctcctgca
    24781 aaggcgatgg agagcatggt gcctcgtgca ccttttccat ctaagtaaac acttgggtat
    24841 ttcatcgttg tttttgcacc taggtttccg tcgatccatt caacggttgc gccttcgtat
    24901 gcttttgctc gtttggttac taagttgtac acattgtctg accagttttg gatcgttgtg
    24961 taacggcaat acgcatcttt tcttgtaaag atttctacga tggcggcatg caggctgtta
    25021 cttgaataag taggtgccgt acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca
    25081 atgatcagcg tacgttcgaa ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt
    25141 aatggaacat cgactttgac gccttttggc acgtaaataa aggtcccacc tgaccatacg
    25201 gctgaattca gcgctgctaa tttgttgtcg gttggcggta caagttttga aaagtactct
    25261 ttgaataatt cagggtattc tttcaaagca gagtcagtgt cagtaaagac gattcctaac
    25321 ttttcgaatt catctttcat attatggtac acgacttctg attcatattg tgcggaagca
    25381 ccggctagat aagctcgttc agcttctggt ataccaatct tttcaaatgt atctttgatt
    25441 ttttctggta cgtcttccca gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg
    25501 atggcatcga aatcgatgtc tgaaaggtcg gggccccatg tttgcattgg cattttgtta
    25561 aatgtttcta gagatttcag acggaattca agcatccatt ctggttcgcc tttgattctt
    25621 gacatctcac ggatcacttc ttctgtcaag ccttctcctg tactgaaaac cggttcgaca
    25681 tcatcgtgaa acccgaattt atattcttct aattctggta cgcccatgtt ctcactcctc
    25741 ttccttgtgg taatgatgga aagctgcggt accatcgttt tccattgctt ttcctagtgc
    25801 tttccaggca agtgtcgcac atttgatccg agcgggaaat ttagctacac caccaagcat
    25861 agcagcatcg cctaattggt cgggatcttt gacttctttt ccttggacaa gctgtagaaa
    25921 gtctacagtt aaatgttctg cttcttctat tgattttccg atgacggcgt ctgtcatcat
    25981 cgaggcactt gccgtactga tggagcagcc actgccgtca aaggcgatat ttttgatgat
    26041 tccgtctttt acatcgactt gcaattcgat cacatctcca catgtggggt tgttcaattc
    26101 gatcttgctt gtcgagtcag tcaagcttcc acgatgatgc ggatgggaag aatgatccaa
    26161 gatcacttga cggtataaat tgtctaattt agatagtccc atgttggaaa aactcctttg
    26221 ttgccatgat tgcttcaact aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc
    26281 tcgcgcgagc agtcgctgat acttcaaggt acttcaatag cggttgcgca caatgatgac
    26341 ctgctctgac ggctacccct tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc
    26401 catccaaatt gaatgcaacg actcctgtcc gaaaattcgg gtcttgtgga ccataaacag
    26461 tcaatccatc aattgcttgt aattttggca acacataagc ggctagttct tcttcatatc
    26521 gatggatatt gtccataccg agtttttgca aataatcgat tgcatggcct aatgcgatag
    26581 cgccggcgat atttggtgtc cctgcttcga atttccaagg tagttctttc catgtactgt
    26641 cgtataattc gacaaaatcg atcatttcgc caccaaattc aacaggttcc atttcttcta
    26701 accattttcg tttaccatat aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc
    26761 tgaaagcaaa gaaatcacag tctagtgctt ggacatcaat ggccatgtgc ggggcagatt
    26821 gagcaccatc tactacgatg accccgccgt tttcatggac aagttcagct aattcttgaa
    26881 caggattgaa gacacctaag acattggata catgagcaat ggagacgatt ttggttttgt
    26941 ccgtaatctt tttcgctgca tcctccatat ccaggaagcc ttcttctgtt agttcgatat
    27001 atttcaagac agcacctgtt cgttttgcca actgctgcca cgggataaca ttagaatgat
    27061 gttccatgta agagagcact atctcgtctc ctgctttgat atagcggtcg ccgaaggttc
    27121 tggctaccca attcaagctt gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt
    27181 ttgcatggat gaacgcccgt actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt
    27241 ctgctaacgt atggacaccg cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta
    27301 atgcctggag tacttgtttt ggcttttggg tcgtcgctgc attatccaga taaactaaag
    27361 gttcatcatt gacgtcttga aataagatag gaaaatcatt gcgtagcttt ttagcatcga
    27421 tcatccattc aacttccctt cgattacttc gactaattct ttccgaactt gttctacagg
    27481 gattgctgtt aagacagaac cgaggaatcc tcgaatcact aagcgctctg cttcttcttt
    27541 atgcaatcct ctactcatca gatagtacat ttcttctgga tcaactcgtc ctacactggc
    27601 agcatgtcca gcagttactt cgttttcatc gatcaaaaga ataggattcg catcaccacg
    27661 tgctttatca gaaagcatca agacacgact ttcttgttgg gcatctgcac cttttgcccc
    27721 tttcaagatg tggccgatac cattaaaggt caatgttcct ttttctcgga tcactccgtg
    27781 ttgcaaaata tgcccgatcg tatgaggggc tttattggtt actcgtgtat cgattccttg
    27841 gatttgtctg cccgagctga ttgccacgat tttgacttct gcgtgtgcac cttctcctgc
    27901 taaatctgag tcaaagtcag cgatcacatg accgtcattc atgacaccaa ttgcccagtc
    27961 gacagaagca tctcgtaaaa tgtgcccacg gcgattcata taagaagtaa tattttctcc
    28021 taattgatct acggctgaat atttgatttt tgaaccagct ttagcgatga cttccacgat
    28081 gatattacct gatgattttg ctacttgttc tttggttgtc tggaaacgtt ccagataaga
    28141 aaattcacta tgttcatctg ccacgatcaa cacatgcttg aagaagtgct catcggatgc
    28201 gccatcttga atgaataagg actcgagtgg ttcatcgatc acgacatttt taggcacata
    28261 caggaaaata ccgctgttca taaaagctac atggagtgca gtcaattggt cttcgttcat
    28321 gtttactgct tttttcatat aatattcttg gactagttct ggatattcga tcattgcagt
    28381 gaacaagtcg gtaaaaatga ctcctttttc tgctaattcc acaggcagtt gttcaaaaat
    28441 cgtccatgaa ccttgttgga caagcacagg attgtctttc attgcatcaa atgccggaat
    28501 cgtgcctgtt tcactgatgg tttgtgtttc tttcacatca aataacgacc agcgatggaa
    28561 tttcacccga tcgatatgcg gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg
    28621 gaaggttctc atccaatcag gttcgccttt acttaaagaa aaatcattta ccgcgtcaag
    28681 ataatcgagc cagttttttt ctttcattct tgctccccct tacgcttctt cttctttata
    28741 gtcgattcct aattctttac tgataccagc gtagccttct gcttccagac gtttagctag
    28801 gtcagcattc cctgtcatga ctactcggcc ttccatcatg atgtgaacga catcaggtgt
    28861 gatatagttc aataatcgtt gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg
    28921 catttcattt actcctttag caacgacttt caaagcatcg atatccagac ctgaatcgat
    28981 ttcgtctaaa attgcaaatg taggttctaa catcaacaat tgcaggatct cattccgttt
    29041 cttttctccg ccagaaaaac cttcgttcag ataacgttca gccatttctt ctggcatatt
    29101 tagtaaagcc attttttcat ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt
    29161 ttcatcacgt ttcgcattga tcgctgcacg catgaattcc gcattcgtga tacctggtat
    29221 ctcacttgga tattgcatag ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa
    29281 gtctaaaaca tttttcccgt caaacaaaat ttcgccttct gttacttcat agtttggatt
    29341 gcccatgata gcggcagata aggtagattt tcctgtgccg ttaggtccca tgatggcatg
    29401 gatttctccg gttttcattg ttaggtttac gcctttaagg atttcttttt cctcgatcga
    29461 cacatgtaga ttttttattt ctaagacaga catgcattat acccccaaat tctctttcgt
    29521 tctcactcat tttagactaa ttgagaatga agcgcaatga ccttccttac taaaatcaaa
    29581 tatcttttat ttattattaa acaattttga tttctaaatt agaataggta taaaaaagat
    29641 gagaaataag ccattacaga cttatttctc atcttttttg ttccatacct tattttgcat
    29701 cagcctttac tggtttaaca gagccgcccc atttgtctgt gatccattct tggacttctt
    29761 tgcggtgaag cacttcaatc agttttttga ttttgtcact gtcttcatca cctttacgag
    29821 tcgcaataat attcacgtaa ggagagttgt ccttttcaag caatacggca tcttctaatg
    29881 gattcaaccc tgcgccgtaa gcgaagtttg cgttgattgc aactaggtca ccttcatcat
    29941 tttggtacgt agaagctaaa agtgatgggt cgatcgtatg gttgaacttc aagttcttag
    30001 ggttttcttc aatatcatcg aatgttgctg tttccagatc tactccatct ttgactttga
    30061 ctaagccagc atcttgtaga attgtgatga tgcgtcccca gtctgattca gaatttgaag
    30121 taatgattgt tgcaccatct tttaattcgg aaacatcttt gactttttta gagtacaagc
    30181 ccattggttc gatgtggaca gcaccggcat tgacaaagtc atatcctttt tctttgattt
    30241 ctttgttcaa ataaggaata tgctggaagt aattggcatc aatgtcgcct tcttccaaag
    30301 attggttcgg taatacatag tcatcgaatt tgacgatttc aagatcgatt ccttcatctt
    30361 tcaataatgg tttcacatgt tccaaaatct ctgcgtgcgg tgtcggtgaa gcgccgactt
    30421 tcaatgtcgt gtcgtctgct ttatttgctg aatctttgct gtcgctgttg ttaccgcatg
    30481 ctgctaatcc tacagtcagc aaaagcgctg ctgcaaatcc aaaaattttc tttttcatat
    30541 cctacatccc cctatatcct attatctttt gtctgttttc ttagttaaga aatcaccgat
    30601 tccttgaacg ataaatacga ttagtaaaat aattaaagtc gcaacgaatg tcactgttag
    30661 attaccacgc tggtagcctt cctgccaagc aagacctcca agtccacctg cgccgatcgc
    30721 gccggccatc gcagtaaatc cgatcatcga gatcgttgta actgttaatc cagaaatcaa
    30781 tgcgggaata ctttcaggta tcaagacctt ccagatgatt tcccagtaac ttgctcccat
    30841 tgcatctgct gcttccagta caccagggct gacttcacga aaagcaatct ctactagacg
    30901 ggcataaaat ggtgctgcgg ataaaacaag agctggaatg gctgcttgcg cacctagcat
    30961 agtgcctacg attgctttag taaatggaat gatcaaaacc atcaggatca taaatggcgt
    31021 tgaccggaaa atattactga tgatcgatac gattccataa atgattcgag catacggttt
    31081 gtttttgcgt ccaatagagt aaaggatcaa tcccaaaact aatccgatga tcgtcactaa
    31141 gaccattgag attaatgtca tatatagtgt atcaaggata gcctgattca tcgtttccgg
    31201 gttgatttgt ttaaaatcta gatatgtttc tgaaaagctt ttatccacga tggatcacct
    31261 caattcctac tccttgttct ttgatgaaca cttctgcttg atctaattct ttggtttcac
    31321 cagtcatcaa gacagtcaat gaaccaaagg aaccttcttt cacttgtttg atctttccat
    31381 acacaacgtt gacatcgatg ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg
    31441 catttctttc gctaaaggtc aaagttgcca aacggccgct tggattttct ttgatgagtt
    31501 cagctaatag ttctgtcgaa tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg
    31561 ttacttcttt tttcggttgt ctgaagacag tcaatacgtc tccttcttct acgactttcc
    31621 ccaactccat cacagcgact ttgttgcaga ttttgcggat cacattcatt tcatgtgtga
    31681 tcaagacgat ggtcaaattc aatttcttat tgatcgctaa caataagtct aatacttctt
    31741 ctgttgtttg cgggtccaat gcgctagtag cctcatcaca aagcaggatc tcaggatcat
    31801 ttgccaatgc tcgtgcgata ccgacgcgtt gtttctgtcc gccggaaagt tgtgaaggat
    31861 atgcttgtcc gcgtccttct aacccgacta aacgcaacag ttcttctgcc cgttctttac
    31921 gtttgctctt tggtacgcca gctaattcca gcggcagctg gatattttca agtacagttc
    31981 ttgaccataa caggttgaaa tgctggaaga tcataccgat tttctttcgg aatgtacgta
    32041 attctttgtt ttttaatttt gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg
    32101 gaagctcaag tccattcagg agacggacta atgtactttt tcctgcgcca gaatagccga
    32161 cgattccata aatatcccct ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt
    32221 ttccgttttt cccattgaat gtctttttga cattcgttag ttggattaat gacatgccat
    32281 tcacatcttt ctcttatgat ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa
    32341 aaggacgaaa gcttgaactt ccgtgttacc accttgattt accgttactt cgcaataaca
    32401 gcctcttcca gtacttggac ttagcttctt tccgtatact gtcgcgctgt aacgggcact
    32461 cccgtagtaa gtttgcttac gctcgcttac tctgctctaa gaccatcttc catctcattt
    32521 tcgttaccca ttttcagcaa acagggctct cttcaaacgt ccgtgacatg tactcttctt
    32581 ttcaaagctt tctcgtttca tataggattg cttatgatta tagcaactgg aagaatcagt
    32641 gtcaactttt ttcaagtgat tttctgattt tataattcgt caaattcttt tggatcaaca
    32701 tctttgaaac ttaagatcca cgcattcatt tcatctgaat cattcaagat attgatatct
    32761 tcaccgattt tatcattaac agaagaaact gtacccgtta gcgggctggc aaattcattg
    32821 accgcttttt ccgcttcaac ttcgatcaga ggttcatctt tttttagcga ttgtcctgct
    32881 tttggcagat tggcaaacgt gatgtctcca agttcttctt gcgcttcatt tgtcaaacca
    32941 acacagtatt cttttccatt aaataagatc caaaggttgt cctttttctt caaatgcttg
    33001 cctgccattt tatatcagtt ctccttcata aattttttca ttgaatccaa ttgcaacaag
    33061 ctggtcctct cgtaagatca gtggtcgctt gatcagcata ccatctgtcg ataagacttg
    33121 gctcgctttt gaagccgtaa actcgcctac ttgatctttc agtccaagtt cccgatattt
    33181 catcccgctt gtgttgaaca ggcgacggat tggtacttga ctcgtttcta gccaagtctc
    33241 aatcgttgag gccttcggta cttctttgat catatcaacg gtttgataac taacgccatg
    33301 ctggtctaac cacgctttgg cttttttgca tgttgaacat ttcgggtacc agtaaaaggt
    33361 gtacaatttt cttacctcct tgaagatctt acaggtgctt cattttggta tctcatcgat
    33421 aatatcaacc cgataccgat catgttccct aaaatcgagg atcctccctg actaatgaaa
    33481 ggcaatggga tccctgttaa aggaagtaat ccgatatttg ctccaatatt ctcaaagaca
    33541 tggaacagca gcatcatgat caaaccgctt gcgatatagg cgtaaaattc attattcgta
    33601 tcgaaacaca cccgaatcat acgatagatt aaaataaaat acaagaaaat gacgaatgca
    33661 ccaccgataa agccgaagtt ttcaccgata acggtaaaaa tcatatcgga ctcacgaaca
    33721 ggcacataga tattgcttac gttgaatcct ttaccaaaca agccaccggt accaattgcc
    33781 aaaagtcctt gggccggctg ataactcatt cctgacgtat catggaaagg gtccagccat
    33841 gaatcaatac gtgcaaactg ataagatttg aatccaactt tataaagcaa gtctcttccg
    33901 ccttcagtcg taaccaagaa gatcgttcct gctccaacga ctactgccaa gattactaca
    33961 ggtgcgatga tgcgccaaga gattccagac atcaggaaaa tcccgccaaa gatcgcaaga
    34021 aagacaagca tcgttccaaa gtcatcttgg gccatgatca atgctaaaac cggtattgtc
    34081 acgatcaaga gcttcccaat cagccaaaag tccgatttca tcgtccgttc ttttacttgc
    34141 gtattatgtt tagttacgat cagtgccatc atcaagatgt acgcaatctt catcaactct
    34201 gctggctgga aagtaaacgc accgaatcga aaccagtttt tagaccctgt tgaagcagct
    34261 aaattctgat catagaattt gagcaagagt cccattacca ctaaccccaa accataaagg
    34321 taaggagtca gtttccagat ccatttggaa ttcaaatgca tgatgacaac gattgctgcc
    34381 gctccaacta aataccagac aacttgcatc cctacgcctt tcaagactgc tggattgttc
    34441 ggatcatggc taagtgctac atataatgag agcagaccaa tcaaacataa cagaaagact
    34501 ggtaaaatca cgccataatc aatacgatta tcgttcgaag cttttaattt tttttccatg
    34561 tttcatccct tttcttaaaa accatactgc tgattattat agcggtccag ttagataatg
    34621 gaaagtcttt ccatagacat ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc
    34681 aaatgcagta caaaagccta ctgcttatct ttagtgtatc atagaacttt ttttgataca
    34741 tgcaattatc taccagctga gattctcctc gctgtgagaa gtttctgtgc tttcattgta
    34801 tcgaaggatc tcttcggcaa tctctagcag ctgatcagaa accagattgg ttcgttggat
    34861 gaaataaaaa tatcgatgga aattcaacgg agtcattggc acattttctg gaacagagcg
    34921 cttagacaaa atcgagcgac cgattccttc cttcagcaac gcaacaatta catcgttact
    34981 tttcacgatc atcttcttat cttggatatt catctcttca aaataacgct gcgtgtaatg
    35041 aaaaacacca gagtttgctt ctcttacaag ccacaatttg ctttttgggt ccccagcgat
    35101 gactagctgg tcttccatca aagagtatct ttgtacgccg gaagtctcta atggcttttc
    35161 gataaaacca aaatcggatt catgtttcgc tacttgttct aatgattcat gagaattcgt
    35221 gatttcgatc tcaaaatgca ctttaggaaa ccttttgatc agatgtttca tcaactgagg
    35281 caaaagatag acagcaaagg tgtgggaagc aacgatccga catacttctt cttcattttg
    35341 ttcattgagc aatttttgct gagtttcttc ccattcttct aacaaaacca gtgtccgttc
    35401 atataataag tcggcttgct tagttggaag catctctttt cttccgttac gttgaaaaag
    35461 atcgatattc aattcccgct ccaactgttt gatttgattc gagactgctg gttgagataa
    35521 aaacagtact tctgctgctc tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt
    35581 taataactta aacatggcac cacttccata actttttatg ataattacta taaaaactat
    35641 ttgatttaaa tatacaaaaa accatctata atagaaatgt acaacaaaac agttgctgat
    35701 ccaactagta acttagatga agacaatcag ctgtctgctt gctcgtaatc atacattcct
    35761 attcatttta caatgtattc gttcatcttg gtactaaaat ctattaaata aaaaggagaa
    35821 tattatatat gaagaacttt ttgaaaatta ttccgatccc gatctgcggg ttgatccttg
    35881 ggttagcttc ccttggaaat ctgtttaaag actatcatca tgttgcttta ggcaatctaa
    35941 ctggcggtat ctctatgatt cttatgatat tcatttttgt taaattgttt atcttattcg
    36001 aacacacgaa acaaacgttg catgatccaa tcatcgcctc cgtttcacca acttttacga
    36061 tgtcactcat ggttatttgt acttattttg tttcatatga aagtattgca ccaattgtca
    36121 aattcatttg gctgtgcgct gttatcttcc aagtgatcct tgtcttttat ttcaattatc
    36181 accatgttat caaagctgat ctttcaattg aagcaattta tccaagctgg tttatcattt
    36241 acgttggatt tggggtcatc actgtaactg ccgggaactt cttcccactt attggaaaaa
    36301 ttttcttttg gatttcattg atctgctatg cgttcttatt gccgatcatc atcaaacgtg
    36361 tctttttcgt aagaaatatg gctcatccga cacgtccgct gattacgatc gttgcagcac
    36421 ctggttctct ttgcctaaca ggttatctta aaaactttgc tgaacctaat ttttatcttg
    36481 tcgtagcatt gttcgcttta tctcaagtat tgtatttcgt tgtgctgtct atgcttccac
    36541 gtttgttcaa gcttcgtttt tatccaagct atgcagcctt tacattcccg cttgttatct
    36601 ctgcaacagc tatatttact actgttcatt acttcaacag tttaggtatc tatagtagcg
    36661 tattggattt cttaaaagtt gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc
    36721 ttgctcatta cgtacgtttc ttaattaaag aacatagcaa acatggaaaa tttgtttcta
    36781 aaaaaatcgc ttaataatcg attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat
    36841 aagaacggcc aaacaaaaac agctgttcat gtttttgtac tggttgggct tattaccgca
    36901 gatgcaagct tttgaacacc gtttaccaag aggtcgtaa
//
LOCUS       DACEKG010000022        35986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEKG010000022 DACEKG010000000
VERSION     DACEKG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.022"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(417..1040)
                     /locus_tag="IW664_001010"
     CDS             complement(417..1040)
                     /locus_tag="IW664_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848389.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1230..2936
                     /locus_tag="IW664_001011"
     CDS             1230..2936
                     /locus_tag="IW664_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0848390.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(3094..3996)
                     /locus_tag="IW664_001012"
     CDS             complement(3094..3996)
                     /locus_tag="IW664_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0848391.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(3993..4697)
                     /locus_tag="IW664_001013"
     CDS             complement(3993..4697)
                     /locus_tag="IW664_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0848392.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(4728..5369)
                     /locus_tag="IW664_001014"
     CDS             complement(4728..5369)
                     /locus_tag="IW664_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0848393.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(5407..5721)
                     /locus_tag="IW664_001015"
     CDS             complement(5407..5721)
                     /locus_tag="IW664_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848394.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(5709..6338)
                     /locus_tag="IW664_001016"
     CDS             complement(5709..6338)
                     /locus_tag="IW664_001016"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0848395.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(6345..7061)
                     /gene="pyrF"
                     /locus_tag="IW664_001017"
     CDS             complement(6345..7061)
                     /gene="pyrF"
                     /locus_tag="IW664_001017"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0848396.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(7058..7990)
                     /locus_tag="IW664_001018"
     CDS             complement(7058..7990)
                     /locus_tag="IW664_001018"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0848397.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(7987..8763)
                     /locus_tag="IW664_001019"
     CDS             complement(7987..8763)
                     /locus_tag="IW664_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0848398.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(8817..12002)
                     /gene="carB"
                     /locus_tag="IW664_001020"
     CDS             complement(8817..12002)
                     /gene="carB"
                     /locus_tag="IW664_001020"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0848399.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(12007..13083)
                     /gene="carA"
                     /locus_tag="IW664_001021"
     CDS             complement(12007..13083)
                     /gene="carA"
                     /locus_tag="IW664_001021"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0848400.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(13088..14368)
                     /locus_tag="IW664_001022"
     CDS             complement(13088..14368)
                     /locus_tag="IW664_001022"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0848401.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(14387..15313)
                     /locus_tag="IW664_001023"
     CDS             complement(14387..15313)
                     /locus_tag="IW664_001023"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0848402.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(15326..16609)
                     /locus_tag="IW664_001024"
     CDS             complement(15326..16609)
                     /locus_tag="IW664_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0848403.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(16623..17162)
                     /gene="pyrR"
                     /locus_tag="IW664_001025"
     CDS             complement(16623..17162)
                     /gene="pyrR"
                     /locus_tag="IW664_001025"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0848404.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(17397..18308)
                     /locus_tag="IW664_001026"
     CDS             complement(17397..18308)
                     /locus_tag="IW664_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0848405.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(18305..18769)
                     /locus_tag="IW664_001027"
     CDS             complement(18305..18769)
                     /locus_tag="IW664_001027"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0848406.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(18795..19472)
                     /locus_tag="IW664_001028"
     CDS             complement(18795..19472)
                     /locus_tag="IW664_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0848407.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDI"
     gene            complement(19459..19845)
                     /locus_tag="IW664_001029"
     CDS             complement(19459..19845)
                     /locus_tag="IW664_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848408.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(19992..21662)
                     /locus_tag="IW664_001030"
     CDS             complement(19992..21662)
                     /locus_tag="IW664_001030"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0848409.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            21845..22078
                     /locus_tag="IW664_001031"
     CDS             21845..22078
                     /locus_tag="IW664_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0848410.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(22177..22608)
                     /locus_tag="IW664_001032"
     CDS             complement(22177..22608)
                     /locus_tag="IW664_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0848411.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            22687..23100
                     /locus_tag="IW664_001033"
     CDS             22687..23100
                     /locus_tag="IW664_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0848412.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(23138..24286)
                     /locus_tag="IW664_001034"
     CDS             complement(23138..24286)
                     /locus_tag="IW664_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0848413.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(24408..25004)
                     /locus_tag="IW664_001035"
     CDS             complement(24408..25004)
                     /locus_tag="IW664_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0848414.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(25204..27846)
                     /gene="alaS"
                     /locus_tag="IW664_001036"
     CDS             complement(25204..27846)
                     /gene="alaS"
                     /locus_tag="IW664_001036"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0848415.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28234..28665
                     /locus_tag="IW664_001037"
     CDS             28234..28665
                     /locus_tag="IW664_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0848416.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(28710..30059)
                     /locus_tag="IW664_001038"
     CDS             complement(28710..30059)
                     /locus_tag="IW664_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0848417.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(30129..31292)
                     /locus_tag="IW664_001039"
     CDS             complement(30129..31292)
                     /locus_tag="IW664_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848418.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            31421..31744
                     /locus_tag="IW664_001040"
     CDS             31421..31744
                     /locus_tag="IW664_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848419.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(31927..33276)
                     /locus_tag="IW664_001041"
     CDS             complement(31927..33276)
                     /locus_tag="IW664_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0848420.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(33345..34298)
                     /locus_tag="IW664_001042"
     CDS             complement(33345..34298)
                     /locus_tag="IW664_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0848421.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(34400..35590)
                     /locus_tag="IW664_001043"
     CDS             complement(34400..35590)
                     /locus_tag="IW664_001043"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0848422.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT        10248 a         7848 c         6273 g        11617 t
ORIGIN      
        1 attataatca ggcaagtatc cagtctacaa aaaaagacaa tggccaaagc ggtcattgtc
       61 tttacacaaa aaaccgcata gattctaatc tatgcggcag tcaatgttca tgtctagcat
      121 agatacaagc tttagaaatg cactctaaaa agttgtatct atgggaaagt ctcaccatta
      181 atacaactta tctacgccag ctgcgccaat tttttttgtt ttctgctgaa atacgttttg
      241 ataagttcat tacaggtcac cctttcctta agaattgatt taagtctaat cgaatttttg
      301 aatttcgtca actatatttt ttaatttttt atttgttatt ttgaagatca aacatcactt
      361 gttctgataa ttaaaaatag cgctatatag acccaccgtt tcatcgcgcc aatgtctcaa
      421 ttagcaatct cggaattttg ggaagttact cccgttctat ccaattcttc ttctaattgt
      481 ttgatattgg ctaacggaag tcgattcagt tcagggatca actccttatc aaaatcagtt
      541 attcctccct tacccgcagt tatccgattt gcttcttttc ctccatcaaa gaaaataaat
      601 gtcacttcat ccgttcgtat gttatatttt tgttccagtt catgtacatc ataaacgatc
      661 ggataataaa agatcggtcg attcaattct tccatgagct caggatcatt aaaaacatca
      721 agcatgtcct gataggtttt cccgttcggt ttgctgaaca tcaccgatat cgcccggctt
      781 tgtttgattt tctgatcagc tttgctgtaa tcaaaaggtt tcaatttgct ggttggtatg
      841 gacgaataaa cgagactatt tacttgctct tctcggattt tttccggcaa taaaaacatt
      901 gcaactgtta acagcaatga taagatgagc agaaaaacaa gacgtcgaaa atacttactt
      961 ggattttctg cgaatgacac tgagaaatgt tcattcattt cttgtactct cgatttaact
     1021 acttcccaaa taacaaacac cttcttttct actttttctc ctatttttta ttatacctaa
     1081 aaatttggat ttcaacgata gaaacactgt tttttatgta attagtccaa gatttttcta
     1141 gacttttgct ttactattcg gccaaattgg tttttcttct ttttttcgtt ataatgaata
     1201 tacttatgaa aagaaatgag gaatgaacca tgtcctttga tggtgtattt acccacgcga
     1261 tggtcaacga attgcgtgaa acgttactat ctggccggat ctcgaaaatc catcagcctt
     1321 atgaaaatga aatcgtgctg gtgattcgtt cacgcggcaa aaaccatcga ttattattat
     1381 ctgcgcaccc aagttatgct cgtgtacaga ttacccaaat cgattatcag aatcctgata
     1441 atccgcctaa cttcgtgatg atgcttcgaa aatatttgga tggcgctatt ttagaagaca
     1501 tcgaacaaat cgaaaatgat cgagtgatcc atttccactt tgctaaacga aacgagttgg
     1561 gtgatctaca aaatatcata ttgatcgttg aattaatggg acgtcatagt acgatcgtct
     1621 tagttaatcg agaaactgga aaaatactgg atgcgatcaa acacattgga agctctcaaa
     1681 acacttatcg ttctttgctt cctggtgtag aatatgttgc tccgccaaaa caagaggtct
     1741 tgaacccatt tagctccgag aaagaaaaaa tctttcagcg gctttcccaa actgaactag
     1801 atccaaaagc aatccaacgt cagtttcagg gaatcggttt cgatatcgct caagagctga
     1861 ccaaaagatt actggaacgt cctaatgaaa agatggttgt atgggacgaa tttttctcag
     1921 ccatcagcca tcagcctatt cctacttttt acgaaacgga aaataaagat tttttcacac
     1981 cgattgctta tcaggtattg tccgaacagg catctgctgt aacagcttat ccaacactca
     2041 gtcaattatt agatagctat tatcacgaaa aagcagagaa agatcgggca aaacaacaag
     2101 gcggagaact gattcgaaaa atcgaaaatg aactaaaacg aaataaaaat aaattgaaaa
     2161 aacgtgaaca aacactcaaa gaatccgaaa acgctgagaa ttaccgtaga aatggcgaac
     2221 tacttactac ttttctgaca caagtaccac gcggcgcaaa agaagttgtt ttacctaatt
     2281 attatgaaga agatcggcca atcaaaatag cactagatcc ggcattgaca ccaaatcaaa
     2341 atgcacagaa atatttccat cgataccaaa agctgaagaa cgctgttaag ctgattggcg
     2401 aacaaatcca agaagccaaa gatgaaatcc aatatcttga atctgtctta tctcaactgg
     2461 aaattgccgg cccaatggat atcgaagcaa ttaaagaaga attgacttca gaaggatacc
     2521 tgaaaaagaa gaacttaaaa aaacaaaaac gtaagaaacc atctcagcct gatcaatact
     2581 tttcttctga cggaacgctc attcttgttg gtaaaaacaa tcttcaaaat gaccagctga
     2641 caatgaagac agctaaaaaa accgactatt ggcttcatgc aaaaaatata cccggttcgc
     2701 acgtcatcat taaaagtgac aagccctcag atgaaacaat tacagaagct gccgagttag
     2761 ctgcctattt ttcaaaatac cgatactctg cccaagtgcc ggttgatctt gtacaggtaa
     2821 agcatatccg aaaaccaaat ggcgccaagc ctggttatgt tatttatgaa aaccaaaaga
     2881 cgattatcgt gacaccagaa gaagaaaaaa ttacagctat gaaacaaaac aggtaatcaa
     2941 tgagtgtttc tagaatcata tttgcgtact ttcccacaac agcaattagt tataaattgt
     3001 ttgctacaaa aagaggagtt gtgacaaaat tctgtcacaa cccctctttt ttgatcaacg
     3061 gtgttcaata aacagcgttc tcatctgctt ttcttagctc tgctggtcca gtaaccgagt
     3121 aagctctttg ataaatcttt gagcgatttc actgagcgga cgtttcgccg aataaatgat
     3181 ataaaattca tttagttcgc cttctgccaa agggattaac cgcaagtgct cgcgaatccc
     3241 tccaatcaca ataccagatc ctgaagcgta agcatcagtt tcaagaacga tattcatcaa
     3301 agtacctcga tcactcgtat aaatcactgt ttcttgatca gggatctcga taaggtcttc
     3361 ataaaaatac gggaaattat ttccatcctg tgtaaaacga acttgaggat aagataacaa
     3421 ttcttctttg gtgatctctg cgtgttcagc taatggatgt ccttgcctta aaaaaatatg
     3481 cgttttgaat tttccaatgc tgcggccact tagctctcct tgttgtaaat atcttctgat
     3541 accagcttga ttatgctcat tcatataaat aatgccgatc tcactgtgct gctctttgac
     3601 ttcttcgatc actctcgctg ttgtcgtctc catgatccga aattgtttga tctcgtcttt
     3661 gaattcctcg agtagctgtg cggtcaaagg acctaagaaa tcataatgct gggcagcgat
     3721 cgaaaaattg tctctctttt cactcgttag atagtgagtt tctaatgtat caagctggac
     3781 caatattttc tcggcacgat ttaagaagtc ttgcccttct tctgttaaat atgcccctcg
     3841 attcgttcga gtaaacagcg tgatccctaa ctcggtttct aattctttga taccagctga
     3901 aagacttggc tgggagacaa acattttctt tgccgcttct ctgaaactac cattgtttgc
     3961 tacagcgacc acgtatttca tctgctgaat attcattgct caaactcctc gaaataataa
     4021 atttctcttc cattcaaggg ctggattggt cggttgttcg gtcgagcata ctgaccatcg
     4081 aaggggagaa gaaactcttg gttcagtttt cctacatgat ccattacaaa ccattggatt
     4141 ggcccgctgg taggcggagt tgtcaatgag ccaatataat ggaaatggct tttttgctcc
     4201 ggaaagaaca catccggatt tacccaatgc tcatgttttt ctaagttcca actttcccca
     4261 ttttcttgat tatacagcca accttcctgt accattagct ggtacatgac acccacaact
     4321 agattctctt ttttgctgtt catatgaaca aaatgaaatt caatatcttc ctgttttccg
     4381 ttgatgatat gttcagaagg caagtggaaa tgaatatctg tcaaataata gcgttctttc
     4441 ttgaactcta cataactgag ttggtcaaaa ggtactaaat gaatcgtgtt tttgaattcc
     4501 ttgtcagtaa accgttgctt ttgataatga aaggaaagcg tgtctccttc tattttggtt
     4561 tcttccccct tggtcaaatg aataggcgac tgcaatggaa attccgcacc tctcgcaaac
     4621 caatcacata aactcgccca attttccgga cctttgtctc cagaatagcc ccattcgaca
     4681 tccatattct ttttcaacta tacttccccc tgcatctcat atcattttta tttttcttct
     4741 ttctttacaa tcgttgagac ggtttctaaa aacgcctctt ttccataaaa ttcctggttc
     4801 aatagttccg gaaatatctc tttaaagaaa tactgaacag aaggccgatg ttcaaagctt
     4861 ttaatcgtag tcactgcctc aatgaacatc tccttataaa ctttttccgg gttatttttg
     4921 atgatttcat caaaagattc ataaagaagg tcaaccttat ctgaaatggc taatagttct
     4981 ccttctacgg tttcatcttt tccttcgaac aaccgacggc gataaacttc ttggaactca
     5041 gctggaatct cttggttgat gaattcgtcc gtcatctttt cttcaactgt ttgcagcatc
     5101 cctcttaact ctttgcttgc gtatttcacc ggcgttttga tatcaccaat aaaacgctct
     5161 gtgtagtcat gattcaatga tttttcatag agcagtttcc aatcgatcgg ttgtccattc
     5221 gcttcttcaa tgtctcctag aacttgcgca atagatgcta cacgatacga atgtgctgca
     5281 actgtatgct ctgtatattt aaaaaatcca ggtgcccttg tgatcgtctc taaattgttc
     5341 aatcctaaaa taaattcatt caaccccata aaagttacct ccctttgaga tttctttttc
     5401 cgaatatcat atcattaaat aactgatatt cgcaacattt ccgctcttta ccatttttga
     5461 aacagctccc ttaccaaaaa atagagaaaa tctatatata atagaaaaaa gagaagagat
     5521 tattctcttc tttctgctaa ccagtcttag aaaaatgaat tttaaaaaaa tcaaaaactg
     5581 atcacgatag aagtttcttt tcatacctga tgaaaagatg cttctcgcaa tcagtttttg
     5641 gatttttgct attccaattg atgattctgg agacacaaat cggatttctc tatcttcatc
     5701 aagcgcgctt aaatctgcca attttcagga tcttttttcc attcttttag cagagttaat
     5761 tcttgctcat cgatataacg atcttctaaa gctgcttcaa tcaatacaga ataattggtc
     5821 aacgtcataa gagggatttc ggctttttcg aaatttgctt ttccttttgg caactcatac
     5881 gtaaagatag ccgcaacacc aaggatatcc gcaccttctc tttttgctgc ttcagctgct
     5941 tccaagacac ttccgcctgt agaaatcaaa tcctcgatca caaccatctt ctgaccttca
     6001 aatatacgtc cttcgatttg gttacctttc ccatgatctt tcgccttact tcgaatatag
     6061 accattggca aatccagtat ttccgctacc catgcggcat gagggattcc tgctgttgca
     6121 gttccagcaa tcacttgtac ttcagggtaa gcttctttga ttttgcttgc taatcctttt
     6181 gcgatctctt ttcggacttt tggatagctc atcgtgattc ggttatcaca gtaaattggg
     6241 cttttgattc cacttgccca tgtaaaagga ttacttggat ttaaaaatac tgcttcaatt
     6301 tctaataagt cttttgcgat actttgttca acattcattt taggttagct ccattctgtt
     6361 tgaatcgttt gatatgcttt ataaggatta gttgactgag tgattggtcg acctataacg
     6421 ataaaagttg agccgatctt ttttgcctga gaaggtgtga ctactcttgt ctgatctcct
     6481 gcgactgttc cttctggtct tatacccgga gttaagcaaa caaattcgtc tcctgccatt
     6541 tctttgatcg cttctacttc ccaagcagaa gaaaccacac catctaatcc tgcttttttg
     6601 gcacagctag cataatggat cacacttttt tctaaagaaa cttcgattag ttgatcggca
     6661 tgcatttctt cttcacttgt agaagtaagc tgagtgatag ctagaagtag cggacgtttt
     6721 ccaccttctg gagtcccctc ttctaatcct cgcattgccg cctccatcat acgaataccg
     6781 ccagcggcat gaacacaggt gagatcaaca ccaagctttg ccaatccccg cattgctttt
     6841 tcgaccgtat tcggaatatc gtgaagtttc agatctaaaa atacattatg accagcctct
     6901 tttagataac gtacgatttc aggtccttct tggtagaaca attccattcc gattttcaca
     6961 aacaattttt cttctttcgg gaaaaatcga agaaagtctt ctacctcttg cctagttgaa
     7021 aaatctaatg caataattgg tcgttggtct agttgattca tcgttcacgt tcctctctca
     7081 cttctttgat taatctttcc agcgattcga tgcctagttc gctcattcgc acagggagct
     7141 ctttaattag tttaggacag atatacggat cggtgaaatt cgcggtccct atagctacag
     7201 cacttgctcc agccataaac atctcaagaa catcatccac tgtctgcacg ccgcccatcc
     7261 caatgattgg aaggctggat atacttgcta cttggtgaat caaacgaata gcgacaggct
     7321 tgattgctgg tccggagagt ccacctgttt gattggctaa aatcggtttt cttgtcttaa
     7381 ggtcaatccg cataccaaga agagtattga tcatcgtaaa cccatctgcc cctccagctt
     7441 cgattgcctg agcgattgga acgatgtctg ttacatttgg ggacaatttg acatagacag
     7501 gaacagaaga aactttcttt actgcttgtg ttaattgaaa agcaacatca gaatctgttc
     7561 caaaagcaat tccgccatgt ttgacatttg ggcaagaaat gttcagctcg attgctttca
     7621 catttggtgc ttcgccaatt ttgctacaaa cttctacata gtcttcttca caagcaccag
     7681 caacatttgc gatgatgggt aactcattgt actcctctaa ttctggaaga agtttatgca
     7741 taacaacgtc tagaccagga ttttgcaaac caattgcatt caacatgcca gatggtgttt
     7801 ccgccactct tggcgtggga ttgccaaagc gggcatttgg agtcgttgcc ttgaccatga
     7861 tcgatccaag ctgattcaaa tcatagtatt ttccgtattc tttgccaaaa ccgaaacagc
     7921 cgcttgccgg catgatagga ttttttaatt ctaggccagg aagtttgacc gctaaaggat
     7981 tctcattcat ataattacct ctcctatttt aaaaatcggt ccttcgtcac aaactttcac
     8041 actttttgtt cctgtttcat cttccggcgt atgacaaacg caagcatagc aagcccctat
     8101 cccacatgcc atgcgggatt ctaatgataa ctggacattt tgatgcgaag aaaacagctg
     8161 ttccactgtc ttcaataacc cgtttgaacc acatgcaaaa acagctgtag gttccgtctt
     8221 cgttcctaac aataaattac cgacatttcc gtgcataccg tatgttccat cgtcagtagc
     8281 gattcttgtt tctcctagac gattaaattc tttttcataa taaacgactt catgtgtggc
     8341 aaaacctaaa aaatgcgttg gcttaattcc tttttcgact aactgttttg ataactcata
     8401 tagaggaggg acaccgattc ctccaccgat tataaatact tcgtctcctg tatgaagatt
     8461 ggacaaatca aaaccattgc ctaaaggacc catgacatct aaatggtctc cggcagaaag
     8521 agtagagaag actctagtcc cctcaccttc cacccgataa atgatacggc aagtatttgt
     8581 ttcgtggtcg atatgattca agctaatcgg tcggcgtaat agcaagtctg cacgcggcac
     8641 gagaatatgc aagaactgtc ctggttgctg catttctttt actagatcac ctttcaatgt
     8701 catttcaaaa atctttggtg ccagctgttt ttgctgaacg atttccatca tttcttgttt
     8761 catctgtccc ctcctgcaac ggtttttcgt ctttttgtct taacatgtgc tcgccgttaa
     8821 atagggttag ttgaaaaagc acgggattcc atcactcgaa cgatcgcatc tgctgtgtct
     8881 aaagaagtaa acaatggaat accatgttca actgcttctc ttcggatgat gaaaccgtct
     8941 ttagaagcat ttccccgatc tttatcaatc gtattgacaa ccacttgtgt tttaccatct
     9001 cttattaagt cgactacatt tttttctgct gcttcctcag aaattttcgc tactgtttca
     9061 acgaccagac catttttttc gaaataagca gctgtatgtt tcgttcctat cagactatag
     9121 ccaatttctg taaaacgttt agctaattct agtgcttctt cttttgtttc gtccgcaatc
     9181 gtaaataata cggctccata ttcaggcaag tgtaatccag aagcttcgaa tgccttgtac
     9241 aaagctttgt ctaattgtct gtcagatccc attacttctc cagtcgattt catttcaggc
     9301 cctaagtaag tatcaacttt ctgcaatttc gtaaaagaaa agacaggtgc tttaacatga
     9361 acttcttttg tttctggata aagtccgttt tcataaccaa gtgtctttag tttctctccc
     9421 aaaatagctt tcgtcgcaat ttgtgccatt ggaattcctg tcaccttact taagaatgga
     9481 acagtccgac ttgctcgagg attgacttcg atcacataaa cctcattttc atggatgaca
     9541 aactggatat tcatcatccc gacacaattc aaacctaatg ctaagcggat cgtgtaatct
     9601 tcgatcgttt tcttgatcga ttcagataat gtttgaggtg gatacacagc cattgaatcc
     9661 cctgaatgga caccagcacg ctcaatatgc tccatgatcc ctgggatgag tactgtttca
     9721 ccgtcacaaa tcgcatccac ttcgcattca ccaccaagca agtaacggtc cactagtact
     9781 ggatgttctg gagatgcttt cacagcatgt tccatgtaat cttctaaatc tcgctggttt
     9841 tcaacgattt ccatcgcacg tccgcctaat acatagcttg gacgaacaag aactggataa
     9901 ccaattctcg aagcgataac cacggcttct tctttacttg tagcagtatc ccctaaaggt
     9961 tgaggaatat ccaactcttt cagcgcttgc tcgaataaat cacgattttc cgcacgatcc
    10021 aagtcttcga tcgttgtccc taagattttt acaccagcag cagtcaaagg ttcggctaag
    10081 ttgattgctg tctgaccacc aaattgaacg attactccta tcggctgttc tagctcgatc
    10141 acattcatca catcttcgaa ggtcaatggc tcaaaatata atttatccga aatagaaaag
    10201 tcagtcgaaa ctgtttcagg attgctgttc atgatgattg cttcgtatcc tgcctgctgg
    10261 attgctttga cagaatgcac agtcgcatag tcaaattcaa ccccttgtcc gatacggata
    10321 ggaccagatc ccagtactag aattgagggc ttttctgatc gtgaactttc attttcatat
    10381 tcataggaac tataaaaata aggtgttttt gattcgaatt ccgctgcaca agtgtcaacc
    10441 atcttatata caggaatcaa atcattttct tttctcatct ggcgaacggc ttcttcgttt
    10501 atttcccaca gctccgccac ttttttgtct gtaaaaccgt tttgtttcgc tgttttcaat
    10561 atcttgtcat ccaacggctg ctgtttcaac tgctgttcga tttctacgat atgcaacagt
    10621 ttatctaaaa agaataaatc gatttttgtc aaatccgcta attcttctat ggtgtaccct
    10681 cttctaatag cttcagctac ataaaacagg cgatcatctt gtgctcgaac cactttttgg
    10741 attaggtcat cgtccgtgat gtctgccaac tcaggaagtt cgatatggtg tgtaccgatt
    10801 tctaaagaac gcacagcttt caataaagac tcttcgatat tccgaccgat agccatcact
    10861 tcaccagttg ctttcatctg cgtgcctaaa agccgttctc cgttttcaaa cttatcaaac
    10921 ggccagcgag gaattttagc tacaacataa tctaatgcag gttcaaattc tgcaaaagtc
    10981 gttcctgtaa caggattttt catttcgtct aatgttaatc caagcgctat tttagcagct
    11041 agcttagcga tcggatagcc agtcgcctta cttgctaacg cagatgaacg agaaacacgc
    11101 ggattgactt caatcacata ataattaaaa ctatgtggat caagagcgag ttggacatta
    11161 cagcctccct cgatcttaag tgctcgaata atcgaaagag aagcatcacg aagcatttga
    11221 tattcatggt cagacaatgt ttgactaggg gcaaatacga tagaatcacc tgtatggata
    11281 cccaccgggt caaaattttc catattgcag acaacgattg cattatcagc cgaatctctc
    11341 atcacttcat attcaatttc tttaaaacct gcaatacttc gttcaatcaa acattgggta
    11401 gcgggggaaa gacttaatcc attttctgat acgatacgta attcttcttc attttcacac
    11461 atacctccac cagttccgcc taaagtaaag gctggacgaa cgatgacagg atagccgatt
    11521 ttatttgcaa aatctacagc ttcttctact gtattgacga tttcagactc ggggattggc
    11581 tgattcaatt ctttcatcaa ctgtttgaat agatcacgat cttctgcttg atcgatcgca
    11641 gaaagctttg ttcctagaag ttctatgttc aattcttcta aaatacctga tgctgccagc
    11701 tccattgcca tattgaggcc tgtctgtccg cctaaagttg gaaggagagc atctggacgc
    11761 tcttttcgaa gaatccttga tacaaattca agtgtgatcg gttcgatata tacttgatct
    11821 gccacttctt tatccgtcat gatcgtcgca ggattagaat ttactaaaac cacttcatat
    11881 ccttcttctt ttaaggcaag gcaggcttgt gttcctgcgt agtcaaattc tgcggcttga
    11941 ccaatgatga tcggaccaga tccaatcacc ataattttct tgatatctgt acgtttcggc
    12001 atctgtttac tgctcctttc ctgcgtccat catttccata aattcatcaa ataaatgtag
    12061 tgcatcatgc ggaccagggg cagcatctgg atgaaactgc acactgaaag cgggataatc
    12121 tctgtgtttg actccttcta ctgttccgtc atttacttct acatgagtta cgatgagttt
    12181 ttctggatca atcgtggctt catcgactgc atagccatga ttttgagaag taaaatcaat
    12241 tcttccggtc gctacttctc gtaccgggtg atttaatcct cgatgtccga atttcatttt
    12301 ataggtatct gcaccgttgg ctaaagcaaa taactggtga ccgagacaaa taccaaaaat
    12361 cggtactttt ccttggattt ttttgatcat ttcgattgct tctggcacat cttttggatc
    12421 accaggtcca ttcgtcagca tgactccgtc tggtgatagc gctaaaatcg tctcggcatc
    12481 tgtgttatag ggaagtactg tgaggttaca gtttctttta ctcaattctc gtaaaatact
    12541 atgtttcaag ccgaagtcaa tgacaatgac tttccgacct gttcctggac ttggataagg
    12601 tttgttagtt gagacttgct gcacttgatt ggttgctaga acggttgctt tcagctgatc
    12661 aaatgcatgt tcgaaggaat ctccagcatc aactatacta gctttcatcg ttccgacttc
    12721 acggattttg cgagtaaggg cgcgcgtatc gatacctgat attccaggaa ttcctttttg
    12781 tttcaaaaat gaatctagcg tcatttggtt tcgccagttt gaagctacac gtgcatgttc
    12841 ttttactact actcctttgc atgttggaga aatcgattca taatcatctc tatttacccc
    12901 ataattaccc accataggat aggtaaatgt aatgatttga ccgttaaaac tttgatcagt
    12961 gatagtttct tgataaccag tcatacttgt tgtaaaaacg atttctccga agacactagc
    13021 atctgcacca aaagcctttc cttcgaaaac tgttccatct tctaatataa gcaaacgttc
    13081 catactttta ttctcccttc tgccacacaa gttttccgtt gacaaaagtc atttgtgttt
    13141 cgccttttac tttccagccg ataaaaggtg tattctcccc ttttgataaa aagtcttctt
    13201 tatcgatcgt gcaagtttgt gtaatatcaa aaactgctac atcagcgggg gcaccaaccg
    13261 ttagcgttcc tgcattcaaa ccaaaaatct cagccggttt aactgccaac caattgatca
    13321 cttgttccaa cgtgaatcga ccagtttcta caaaatgagt atagatcagt tgaaacgctg
    13381 tttcgcttcc tacgatacca aatggtgatt tcatgaaact ttggcttttt tcttctaatc
    13441 catggggggc atggtctgta gcgatgcaat cgatcgtacc gtcaagcaag ccttcgatca
    13501 aagcttctct gtcctcccgt cctcttagcg gcgggttcat tttccaaaag ccgaaatctt
    13561 cgggaatatc ttcgtcgatc aaaataagat gatggggtga aacttctgct gttacgtgga
    13621 ttcctgcttt ttttgcgtct ctgattacac ggacactttc tttagtagaa acatggcaaa
    13681 catggtaatg aacaccagtt gcttcagcta gcaacagatc tctagcaatt tgagaagact
    13741 cggttacgct taagatcccg ggaagaccaa gttcttctgc cttcttgcca gcgtgcataa
    13801 cgccgccaaa taataaagat tcatcttctg tatgggctac tagggcttta ttgttttttg
    13861 cagcttcttt catagccaga tacatcgtcc cagctgtttg gacacctaca ccgtcatttg
    13921 tgaaagcaaa agcaccttct tcaatcagtg cttcttgatc aactaatttc tcacttcgca
    13981 gattttctgt gattggcgca tactgcaata ccttcacaac tgcatctttt ctaatgatgt
    14041 catatacttg tctcagcttt tctgccgtat caggtacagg atttaaattc ggcatcgcgc
    14101 atacagtagt gaaaccgcca cgagcagctg agcgagtccc agcttcgatc gtctccttgt
    14161 atgtaaatcc tggctctctc aaatgaacat ggacgtccac taaaccaggt gtgatcaatt
    14221 gtccctttgc atcaaacact tcatcaaatt cggcttctga gaatcctgta ccgattgctt
    14281 tgattcgccc atcttctatc caaatttctg ctggtgtcgt catatttttt cgtgtattga
    14341 tctgaccgtt tttaatgagt gttttcattt tttcatcctc ctataattac gcttttccat
    14401 ttagcactgc ttctaagatt gccattctga cgaatactcc attgctcatt tgctgaacga
    14461 tccgcgactg taatccttcc actaatgaat cagctaattc tacatcacgg ttaactggtg
    14521 ctgggtgcat aatgattgca tgtttttgca acttcttcgc acgctcaact gttaacccat
    14581 gttcctgatg gtagccttct tttgagaaac tttcttttcc atcatggcgt tcgtgttgca
    14641 cccgaagcat catcatgaca tcaacttgat ctagtagttc atccaatggc atataatgtc
    14701 cgtacacttc gaattcatcg tcgtaccatt ctctaggacc tgagaagaat acttgtgcgc
    14761 ctagacgttt taacatctgc atattggatt tagctacgcg agagtgagtg atatcaccta
    14821 cgatggcaac tttcagacct tcaaaatgac caaattcttc ataaatggtc atcaaatcaa
    14881 gcaagcattg ggttggatgc tgtccgcttc cgtctcctcc attgataatc gaacactgga
    14941 ttgttttact ttgtatcagt tcgtcataat agttctcatc accatgacgg ataaccgcta
    15001 cgtctacgcc taatgctgac atcgtcagta ctgtatcgta tagtgtttcc cctttttgga
    15061 cagagcttgt gcttgcttca aattcaatca cttctagccc tagttttttc tccgctacat
    15121 cgaagctttt atgtgtgcga gtactatttt cgaaaaagag gtttgtagca aagtactggc
    15181 gcttttgcgg agtccaatta gcaccttgct tgaattcctg ccctctgcgg atcaagccca
    15241 taacttcttg atcagtaagt gcctcaacgg tcaataaatg ctttaagctg atacgttctg
    15301 atttgatgat cataatgttt cctccttagt tttcttcgct tcgtgaagtt tctggtaaga
    15361 taagatttaa aatgatgcca agtacagttg ctaatgccat tgccgacaat gtaaatgtgc
    15421 ctacttcaaa gacgagtccg ccaattccta ctactaagat aactgaagca atcaacagat
    15481 ttttcttttt atcaaaatcg attttgttct cgattaaaat cttcattcca cttgcggcaa
    15541 ttactccaaa taagataaaa ctgattcctg agatgactgg tccagggata cttaaaatca
    15601 aagcgcttaa ttttcctacg aatcctaaaa tgactgcaaa tactgcagct ccaccgatca
    15661 caaacacgct atggacacgt gtgatagcca gaacaccaat gttttcaccg taacttgtta
    15721 caggaggacc tccgacaaag gctgccacga tttgtgctaa tccatcacct gtcaacgtct
    15781 tatgaagacc cggttcttca aagaaattac gtttagttag tttattcaat accataagat
    15841 ggccgatatg ctctgtcatc gtcacaaaag cgatgggtgc cattgttgtg attgctccaa
    15901 gatatagctt tggttcatat tgtacaaagg gaatctcaaa gttaggaaca gcaaaccagg
    15961 cagcgtctac gattggctta gtatccacga ttccaaacag caaggctacg atgtatccgc
    16021 taataatacc tagtaaaatc ggaatcaatc cgagaaaacc tcttagccac atgttgaaga
    16081 aaattgttaa tcctaatgtg atcagcgcta cagcaatata cttgaaatca tacgtgccct
    16141 gattatacat agcatttgtt gctgcatttg ccgcaagtcc caacccgata accatgatga
    16201 ctggaccgac gacgattgga ggcaagattt tatccagcca tgctgaaccg atctttttga
    16261 tgatcagcgc aacaatcaaa tagactaatc cagttgtgac tgctccttgt gcaatcgctg
    16321 gataaccatc tgttttcatc aaaagctgca tcgctgcaat gaaagcgaag ctgcttccta
    16381 agtacgcagg tatctttcct ttagtagtaa ataagtacac caatgtacct agtcctgaac
    16441 tgactagcgc aatacctgga tcgataccta ctaagatggg gactaacacg gttgctccga
    16501 acatcgtgaa caagtgttgt aaacttaatc ctacccaatg tcctggggtt gggcgatcgt
    16561 ggatatctaa caccacgtca tcattatgaa atcctttttt ctccgacatg tttctctctc
    16621 ctctaatctt cctctttcaa aatcatgatg cgatcttgtc catcaagctc ttgcatctct
    16681 actaaaatct cttccgcccg agatgttgga atgttcttcc caacataatc tgcacggatt
    16741 ggcaattctc gatgcccacg atcgaccagg actgctaatg aaatttttct tggtctgcca
    16801 aaatccatta ctgcgtccat tgctgctcga atcgttcgtc ctgtatacaa aacatcatca
    16861 atcaaaataa cttcttttcc ttctaaggaa actgggatgt cagaagaatg aagttccggt
    16921 tcttctggat tttctttttt gtcatctcgg tataaagtga tatccagttc tcctacagga
    16981 atatcgatat cttctagctg cttcaggcgt tcggcaattc ttgatgcaat gtagattcct
    17041 cgtgttttga tacctaccaa tacgatatct tggatactat ggtttctctc gataatctca
    17101 taagtaattc gagtcaatgc tcgcttcatg gttacctgat ctactacttc ttttgcttgc
    17161 attttcattc cccctacttt tttgcataaa aaaactcctg ccctaagagg acaggaggaa
    17221 tgtttagact gagcgtatac tgatacactg atagctatac tatgtcaatg agcaatctac
    17281 ttatggcgca ccatacgtat ccttgctccc acccttctta gcctcacggg actaaactta
    17341 aaggaattct attaagtttt atgctttcgt tgttaattac tataccgtta tctaaattaa
    17401 aatgcaatgt tttttcttaa tttttctaaa gttttttcaa aaacttctgg aagaggtgct
    17461 tcaaagatca tcttttcgtt tgtagtcggg tgcgtaaaac ctaacatttg cgcatggaga
    17521 aactgaccat tgcctttcaa agttttcttc ggtccgtata ctggatctcc agctaacgga
    17581 taaccgatat acttcatatg cacacggatc tggtgtgttc ttccggtttc tagttttaat
    17641 tcaaccagag taaatccttc gaaacgttcc aatacagtaa aatgtgtgac agccgacttc
    17701 ccatcttctc ttatcgcctg cattttccgg ttttccttcg aacggccgat tggcgcattg
    17761 atggttcctt tttcgtgagg aatctctcca tggactaaag caacatattt gcgcaaagag
    17821 gtcttatctt ttaattgctg agctaatgat tcgtgtgcct ggtcattttt ggcaaccatt
    17881 agcagtccgg aagtatcttt gtcgattcgg tgaacaatac ctggacgaat aacgccgttg
    17941 atcgtagaaa gatccttgat atggtacatc agcgcattga ccattgtgcc atgcgtatga
    18001 cctgctgaag gatgaacgac cattccttga ggtttgttta tcaccgctac atcttcatct
    18061 tcgtaaacga tcgtcaacgg gatattttct gggaccacat ctaatgtttc tggttcgggg
    18121 atcaagacag taatctcgtc tccttgtttt actttgtaat ttgctttgac tggctgtcca
    18181 ttgacagaaa ccgctccatt tttcagccac agctgaattt gcgaacgact gtaatcggtg
    18241 aataaattag tcaatacttt atcgatccta ccagattcat ggtgaatcgt cgttttaagt
    18301 tctgtcattt ttttctttga tccttttcat ctaaaataat ataaattaac atacaaatca
    18361 ctccacatac aagtgtcaca tctgctacgt taaaaatcgg aaagctcata aaatctgtct
    18421 ggaacatatc tactacatag ccaagacgaa tacggtcgat aaaattgcca atcgcaccag
    18481 ctagaatcaa actcaatcca atcatgaacc agcgattacc tttttcacga tttttaatca
    18541 tcaatgtgag aatcacaacg actgcaatga gtgtcactat agtaaagaac cacatcttcc
    18601 cttctagcat actccatgcg gctccagtgt ttcgaatatg attcagtgac aaaatacctg
    18661 gtataagatt tttcgtctct ccaagcgcta gattatcagt gatccaccat ttgaccaact
    18721 gatccactcc tactagcaaa gcgctgaata tccaataaat aattaacaaa aaatcaatcc
    18781 ttttctattt gtttttaaat atcacgaggt cgtacgatcc cgagcagatt accatcggga
    18841 ttcccatctt tagtgataag tacggcttcc aaagtccctt gctcacgaaa catcgcttca
    18901 acctcgtaaa tatgtgtttt agctggaaca aaatgataat ttgtgtactt tccgttttgg
    18961 atgatcaaat ccaacgctcg tttatttgtt aaatcaaccg gacctttttg gctttctttt
    19021 gctagccaaa acccgatatt acgtaaagta atcaatgcta caaattttcc tttattgtat
    19081 aacggaaatt gtgaatacct tttttctgct actatcttta acagttcaag taacggaaga
    19141 tcttgttcaa aacctgttac acgttttgca aatcgaggca gcacggtttc aggctgaatc
    19201 agttcttgtt cgatcagctg gattcgcttg actgtccaat catttggctc tgcaatgaca
    19261 aaatcgactg cgatccgatc atggacgatc gcattcctta actgagcaag ctgtaaaaga
    19321 tcatcttcgt attttctgat cattaattgt tttctttgag ctaggcgtct gacaagttcg
    19381 gtaaatccca tatttcgtgc atttcccatc tcgtctctca tccatttttc gattcgattg
    19441 aaactgatta aaaaaagatc agagtttgcc ataattttcc tccttgatca aatatgcccg
    19501 gttttttata tagaaaaaga acgcaatcaa cacaataact gcaaaaaaac tgaaaaataa
    19561 attttgtagg aaaacggccc ccaacccagt tgctagaaat aaaaacggat agatttgaag
    19621 aatcaagtaa ttcattgtga tcacataagc aggctttgct tctttcactg tataaacggg
    19681 gctttttagc caataaaagg tgacctgcat cgtaaagggc acttccagat caagcgttat
    19741 cttttgttca tgactgagaa cactgcgttt ttcattgttg attcttagct gaatcgagct
    19801 acccataccg taaaaaccgg tttttcttat aaatgtcgtt ttcatcatac cctcctatta
    19861 tgctttagta tagctcaaaa tacaataaag aaaaagaatg atcctagtgt aaacaaaatt
    19921 aattccctat cgtctaaaaa aagaagctgt agtcaaaata cagctccttc gatggtagat
    19981 tataatcaag cttaaaacaa tcccaacact tctccgtcat ttgtcacgtc catattcaat
    20041 gcagcaggtt ttttaggcaa accaggcatc gtcataacat ctccagtcaa ggctactaaa
    20101 aatcctgccc ctaattttgg gatgatttcg cggatcgtga tggtgaaatc ttccggtgca
    20161 cctagcagtg aaggatcatc agaaaatgaa tactgtgttt ttgccataca aatcggcagt
    20221 ttgtcccatc cattcttcgt aaactcttct atttgttttt gcgctttttt gctgaaaaca
    20281 acaccatttc ctccgtaaat tttcttgacg attgtttctg tcttttcttg tatcgtttct
    20341 tctaggcggt ataatggctt gtaattagct tcttttctat caatcaatcg tactacagct
    20401 tttgctaaat caacgccgcc ttctgctcca tctgcccata cactcgctct cttggcaaga
    20461 atcccctgat tttcacacaa gtgctccaac agtgtgagtt cattgtctgt atctgttacg
    20521 aattcattga ttgcgacgat cacaggtagc tgatattgtt ccatattacg tatatggcgc
    20581 tttaaatttg caaaacctac tttcaaagca tcaagattct catttttaag ttcatctttt
    20641 ttcattccgc catgcatttt caatgcacgg acagtggcga caatcaccac agcatccggc
    20701 gcttttttca agttaggtac tttgatatct aaaaatttct ctccgccaag atctgcacca
    20761 aatcctgcct ctgtgacaac gtaatctgct aatctcaatg ctgtcttggt agctaaaata
    20821 ctattgcagc catgagcgat attggcaaac ggtcccccgt ggacaaaagc tggtgttccg
    20881 taaattgttt gtacaagatt cggcttgatt gcatctttca gcagtaatgc cagagcgcct
    20941 tctgctttca agtctccagc agtcacaggc tgacggtcaa atgtatagcc gacaacgatc
    21001 cttgcaagac gagcctttaa atcttcaagg tctgccgcaa gacacaagat agccatgatt
    21061 tcgctggcaa ctgtgatatc aaacccatct tctcttggaa caccttggat tggcccaccc
    21121 aagccaacaa ttactttacg cagttcacga tcattgagat cgactacccg tttccaaatc
    21181 actcggcggg catcgatgtt caattcattt ccttgatgaa tatgattatc taaaagagca
    21241 gataatgcat tattagctgt tgtgatagca tgcatgtcac cagtaaaatg caagttgata
    21301 tcttccatag gcaatacctg tgcatatcct ccgcctgcag caccaccttt gattcccatc
    21361 acaggaccaa gtgagggttc tcgcaatgca ataacagatt ttttattgat tttattgagc
    21421 gcgtctccta accctacagt gatcgttgac tttccttcgc cagctggtgt tggattgatg
    21481 gaagtaacta agatcaaatg cccctcagcg ttatcctttg atcgattgat tccagaaaaa
    21541 ttgattttcg ccttatattt cccatacagt tccaaatcgt cttccttaag atctaattta
    21601 ctagccactt caacaatcgg ttctagctct gtttcttgtg aaatttgcag atcattcttc
    21661 atttgattac cccttttcac gaactttatt caacaaaata tcttctttgt taagatatgc
    21721 caattatacc tttttttatt cagaaaaaaa acataaaatt acaggttttt aatttatttg
    21781 acgtacttat aaatactctt tataatgaag gctatgatca gactttagaa aggtcgtgag
    21841 tgctttgacc acaggaattg taaaatggtt tgacaacaaa aaaggatacg gctttatcag
    21901 ttatgatgac acagaagaaa tttttgtaca ttttactgcg attgaagaag agggattcaa
    21961 aactttggaa gaaaatcaag tggtcgaatt tgagatcatc gaagggaatc gaggaacaca
    22021 agctgcccat gtcaagaaag gcaacgttca gactaccgat actagtatgc atgaatagat
    22081 aaacaaatag agtgagacgc taaacgggat gaaccgtacg tctcactctt ttcttttcct
    22141 gtaaaaattc gtttttttag gttctgattt ttaaaattag ctttttcttt ttttcgtata
    22201 aggataagga attgattctg atttgggtcc gtttttttca aaatgatcaa aaaggactac
    22261 ttgatctttt tttcgcatca tgaaaacaga tggggctttc tcatcttgca acatcatttg
    22321 caccccagta ttagttggcg tcaaagatcg aatctgtccg acagatattt ctcggtcttt
    22381 ccaaaaagca gcataacgta taatcacaga gtttgatttt aaaataaaag aacgccgcaa
    22441 acctagaaat agaaaaacaa gaaaaatacc taaaacaaaa ttgcttttcc aataaggacc
    22501 tgtgttttct aaagaaagaa tcagactaaa aaacagaatg atcaaggtca atgaccaata
    22561 gatgatcgta tgtgccagtt caggctgcca tcgaatattt tttttcattc tctaacctct
    22621 caccgattca gtaatgatat aatcttacca taacattagt ttgaacaaaa aggagaatct
    22681 ctttttatgt taaaagtata cattgatgcc tctacaaaag gcaaccctgg tcctagtgga
    22741 ggtggaatct tgattgtcta tcaaggtaaa caagaacagc tatctatccc gctttctata
    22801 gggacaaacc atcaagcaga gttcgaagtc tttttaaaaa ctttagaatt tctaaagaaa
    22861 aacgacttac agaatgaaac gattttctgt ttttcagaca gcaaaacact agtttctacg
    22921 gtcgacaaaa acgcaacgaa gaacgagctc ttccttccct accttcaaag aatccaagaa
    22981 cttctatccg aattttcttt attgatcctg caatggttgc ccgaaaacaa aaataaagga
    23041 gcagataatc ttgcacgtca ggctctccat aaacaattaa aagaaaaaaa gactctttaa
    23101 attaaaaact ttaaggagtc tttttatagt ttctcattta atataaaaga taattgtgat
    23161 caaacgtaat ttccaattgg taatctccga ttttttcttc tatttgacta cgtaagattc
    23221 tttctagttc tgtatcaaca aactggaatt tctcagggac cactacatca aattgcagcg
    23281 tttttccttg atgaattact ctaaaatcat gaatattaag ccctgtacct gtttcaagaa
    23341 gaatttgctt taactgccgc aagatattat agtatttttc gttatcaaca ggcattggat
    23401 ccaagtgaca gacaagttcc acatccaaag actttttaaa gtctttttct atcagatcaa
    23461 taatcttatg agccacattc aaattcattg attcatctac ttcgatgtgg actgatgcaa
    23521 agcgtttatt gggtccatag ttatgcacca ataaatcatg atagccaagt atatcatcgc
    23581 aagcatttaa atgctcttcc atttcttcaa tctctgctgg cgttgggcga cttcctaata
    23641 gttcgtatac aaaatttcga agcatcgtca atccactata aaggatatat aatgctaaag
    23701 caaaacctat atatccatca atacgccatc cagttaccca ttcgattgct gctgaaagca
    23761 atacagccag tgtagtaaag acatcattat aactatctgt tgctgtagct ctcaatgttt
    23821 cagaatcaat ggattgcgca ataacctgat acattttgcc ttgtgcaaat ttcaaaaaga
    23881 tggaaatgat taggacaaaa aagacgatgg gtgttaaaga gacattttct ggatggatga
    23941 ttttttcagc agaagactct aaaaattgaa atcctacata agtaatgatg atcgaaacaa
    24001 ataacccact gatatattcg aagcgttcgt gtccataagg atgttcctga tctgctggct
    24061 ttgcagcaat tttaaatccg atcaaagtca atatggatga agcagtatct gatagactgt
    24121 tcactgcatc agccataatg gagacacttc ctgaaaaaag cccgatcatt agcttagaga
    24181 caaacaagat cagattcgta accaatccta agatacccgc tagtactcct ttttttgtcc
    24241 gctgttgcgg tatttgtttc tttttttctt cttgtttctt agccaagttt tttacccctt
    24301 ctagtttgct tgttgctcag gtaaaaagat ggagaggacg attgttttcc tctcctctct
    24361 tcctttttct atctatcgtg cgtcctatta ctattaaaag atggtcatta ttctggtttc
    24421 aacggtgttg ctgcaaataa cgcttttgct agtaatggtg ttatgattcc cgcacctatt
    24481 gcttctggta tcccattgat tgctgcaacc cctgctaagg ttttaaataa aagagcagag
    24541 tctacaccat aggcatctgc cgttgcacct gtataaaata atcccatgaa tcctaacact
    24601 aagactgtat tgaccaatga acccagtacc cctgcaaagg ctgagctgat cgttattgat
    24661 ttattttttt gataaatcca gtggaaaacc aagcctgcta ctaagccaac aagtactctg
    24721 ggaactaccg agataatcgg attcgtgaac actaatgtat caaatggcgt tgttggcatg
    24781 gcataggcac gaacaattgt agcgattccc caaaacaagc caatcaccat gccatctttt
    24841 gggcctaata ctactgcagc aataattact gtgatatgga taattgttaa actaatgaat
    24901 cctaatggaa taaaccccag ccaaggaacc atcgcttgta cgatgatgat tgctaacaag
    24961 atcgcacgta agactagtcg aaaggtctta tttctactat tcattttttc accccttttt
    25021 atttatacta atcgtaaaaa tttctgtcac tttcgtcaac taaagaccat ccgaatccga
    25081 ccatttgatt agatgcgcca tccaattgaa aaaagtatga gggcaagact tgtttgtctt
    25141 cgcccccata atcatgtttc gttcaatgtt tgaacttttg tttttctgtc aaacgtaaac
    25201 gccctattgt tgcgcaaccc attctttcgc agctttaagt gcatctggaa taccagcagg
    25261 atttttcccg ccagcttgtg ccatgtctgg acgtccacca ccgccaccgc ctactttcgg
    25321 tgcgattgct ttaatcaagt cgccagcttt tagtccagct tcatttgatt ctttcgtcat
    25381 tgccgctagc agactgactt ttccttcttg ttcgtttgct aaaacaagaa tatctgaaat
    25441 atttttttgt ttccattggt cagctaattg acgcagctga ttcatatctt tcacttttac
    25501 ttgtgctgca atgattgttt ttccattgat cgtttctgga tctttgaata catcaccagc
    25561 ttgttgattc gctaacttgc tagctaattg ttcattttcc ttttgtagct ctcttacttg
    25621 ctgttgcaat tgttccgctt ttgaaacaac ttctttcaat tgaggtgatt taacaattgt
    25681 tgctacttgt tttagttgtt tttcttcttc ttcaagtagt tcgtacgctt ctttacttgt
    25741 tacagcctcg attcgtcgca cgcctgcacc aattcctgat tcagaaacga ttttgaagat
    25801 tccgatgttt tctgtatttg ctacatgtgt gcctccgcac agttcgattg accagccgcc
    25861 aatatttact actcgaacgt catgtccgta tttttcacca aacaaagcca ttgcacccat
    25921 gtttttggct gtatcgatat ctgtttcaat tgtttcaact gggatagctt cccagatttt
    25981 ttcgttgacg attctttcca tttcagctaa ttcatctgaa gtgatttgac caaagtgggt
    26041 aaagtcgaat cgtaaatgtc caggagcgac taaagaacca gcttgatttg catgctcgcc
    26101 aagtacttct ttcaatgcct tgtgcagtag atgggttgct gtatggtttt tgatgatccg
    26161 attgcgcatc gattgatcga tcaatagatg ataggtttgt ccttcactca ttggtgcttt
    26221 tacttctacg gtgtgcataa attgaccatt aggcgctttt tgaacatctg taacaactgc
    26281 tacgatttcg cctacttcat tcatgatcca tccttgatcc gctacttgcc cacccatttc
    26341 tgcatagaaa ggtgtttgat caaagatcat ttgagcttca ccttctgcga cttcattcaa
    26401 aagagactca cctttaacga tgaccaataa ttgactatct gattcttgtt ctgtgtatcc
    26461 aatgaattta ctttctactt tgatatctgt caataaagca gattgtaccc ccattgattt
    26521 ttcagtactg cgggcagagc gagcacgttc gcgttgtgca tccatttctt tttcaaaacc
    26581 agcatgatca actttcagtc cttcatcttc tgccacctct tccgttaatt ctaccgggaa
    26641 gccatatgtg tcgtatagtt tgaagatgtc ttttcctgct agagtcgtcc cgttttcgtt
    26701 ttttacttct tggatcactt gatttaagat ttccaaacct tcattgattg tttcattgaa
    26761 acgttcttct tctgtacgga taactttttc aataaattct ttttgttgaa gtacttcagg
    26821 ataataactt accatgattt ccccaacaac agggactaaa cgatataaga atgcttgttc
    26881 aatacctaat tttttcccat gcatgacagc acgacgaagc aaacggcgta agacataccc
    26941 tctcccttca ttcgatggca atgcaccgtc gccaatagca aatgaaaggg cgcgaatatg
    27001 atctgcgatt actttgtaag aaatatctgt tactgggttt tcaccatatt ttacttgttt
    27061 gcttagcttt tctacggcgt ggataatagg cataaacaag tctgtctcaa agttcgttga
    27121 cgcatcttga acgattgaga ccatgcgttc caatcccatc cccgtatcaa tgtttttatg
    27181 aggaagtggt tcaaatgtgt tatcaggctt atggttgaat tctgaaaata ctaagttcca
    27241 gatttctaaa taacgttcat tttctcctcc tggatagttt tcaggatcgt cttctgctaa
    27301 atcgttataa gcttctccac ggtcatagaa aatttccgta tccggaccac atgggcctgc
    27361 accgatatcc cagaagttgt cttctacatc aataatgtga tctagtggca ggccaacttc
    27421 ttcatgccag atgcgttttg cttctgtatc ttttggataa actgttacat aaagtttttc
    27481 tggatcgaat gccatccatt ttggatcagt caaaaattcc catgcccagt ggatggcttc
    27541 atttttgaaa tagtcaccaa tcgaaaaatt acccagcatc tcaaacatcg tatggtgccg
    27601 tgctgtttta ccgacatttt cgatatcatt tgttcgaata gatttttggg catttgtgat
    27661 tcttggattt tctggcacaa ctgaaccatc aaaatatttt ttcaatgttg caacaccaga
    27721 gttgatccac aataaggttg gatcgttcac ggggatcaaa gacgcactag gttcaatcgt
    27781 atgtccttta gattggaaga aatcaagaaa catttgtcga acttgtgcac tacttaattg
    27841 tttcatgtca ttacctgctt tctataaatt aaatacaaaa aaacaccatc acagccaagg
    27901 acgaaaactc gcggtaccac cttgattgca atgatgtgta atcattacct cttaaactcg
    27961 ataacgctga gctgcgtcgg tatttctacc aaaatagaaa aagggagcaa tttgattagt
    28021 gactattctt ttttcagcca agaaagaatt ttctgtgagt cttaaaaaac aaatcatatc
    28081 cttttcgtac ttatttagta tacgcattct attgatcact gtcaatatct caatttttat
    28141 tttcatttcc ttttggtcat actccacgat ttttggtacg ataagaataa aggaatcaat
    28201 acatctctgc tattcatgac aaggaggaat atgatgaaaa aaatagttgg agcggatgaa
    28261 tggatacgcg cagctagctg ctttttgaga tatcaagttt ttgtattaga acaagggatt
    28321 cctgcagaac tagaattcga cggaaaagat atcccgcaac aaacctatta tctatttctt
    28381 gatcaaaaaa gaccagtagc tactttgcgt tatgaaaaga aaacacctac ttgcttcaag
    28441 ccggaacgat tttgcgtagc aaaagaatac cgaggaaaag ggttaggcgg tcagttgctt
    28501 gaagctgctg aaaaacgggc ggtttcagaa ggttgtcaga ctagttatgt agtagctgaa
    28561 atgacggctg tagattttta tgaaaaacaa gggtatcata cttgcacaga accttttatt
    28621 gaagatgaag tactttgtat cggaatgaaa aaaagtctag cttaaaaaca agagattgtg
    28681 acacgccttt tgtcacaacc tcttgttttt tatttatgtg aatttttctt atttttacga
    28741 gcagtacggc tttgctgtct gcgttcgatt ttgcgttctt ttttattttt ttctgcaatt
    28801 gcccaatcga tttttttctt ataaccaggc ttgatctttt tctttttctt cttcacaaga
    28861 ccaatcagcg tgggatctaa tacatctttt gatttttctc gtttggcacg tctattacga
    28921 tcgtatgttt tgacgatttc cccgtttttg atttcttttg gttcaaatga gacgcccaat
    28981 ttttctatcg catcaatggc ttgctcatca tttggcgaat ataacgtaat ggccgttcct
    29041 tttaagccat tacgtccagt acgtccgaca cgatggataa agaagtctag atcttctgga
    29101 atttctgcat taataacatg agagacacct tcgatatcga tccctctagc agctaaatca
    29161 gtcgctacaa catattgata ctccagattc tgtacttgtc gcatcactcg ttttctctct
    29221 cttggggtga tgtctccatg aatctttgct actttcaagc cttggttttt caagtaatcg
    29281 gcaatttcat ccacatgctg ttttgtattg gcaaatacga ttgctaaata aggatgacca
    29341 attgtcagca gttgataaat catttgattc tcatctttac cttttgttga aatcagccaa
    29401 ttttcaatgt cttcagaaat aactgctttt ggtttgattt cttcaatgat tggattttcc
    29461 atgtattttc gtaagaatgg acgaagtttt tctggaatag tggcagaaaa aactaaaaac
    29521 tgcagctttt ctggtaatcg acttgcaatt tggtctactt catttaaaaa acccatatcc
    29581 aatgtcatat ccgcttcatc gacaacaaat gcaaatgcgg tgtgaatctt cagcgcttgt
    29641 tcattcatca tgtctaatat tctaccaggc gtgcctacta caacctgtgg ctgttggtga
    29701 tgcagacgat ttaattgacg ctgtttgtct gtaccgccga caaaattcga gacacggatc
    29761 tccggtgtag aaaatttagc gatttgtgta gctgcttcat agatctgtgc agctagttcc
    29821 ctacttggag ctgtaataac aacttgtact tcctcttttt ctgcattgat tttttcaaac
    29881 aaaggcaaca aaaacgtatg tgtttttcct gtaccagttt gagattgccc aatgatattt
    29941 tttccttttg caattaaagg aatcaaacgt tcctggacct ctgtgggctg tttgaagccc
    30001 tttgcggtta acgcttcatt gataaacggt ttgaagcgga atatatcaaa attattcatt
    30061 cgtgcacctc ttctctttaa attatcacga ctttggcatt ataacacaga caaagcgttt
    30121 ttgctcagct aatttcgaga atcatcatcc tttgtttggc caatcgtgct aacagtatat
    30181 gtgtgactag ctttttcata aaacagaatc cgtttttttc tcaaaaatga tagcaagata
    30241 tttaaaataa aaataccttg tagtaacaag acaccgagag aaatgaatac tgcactcatc
    30301 tgcaacaccg tattcgttgt cgaattcatg atttgctgaa gcaactgccc caaccagctt
    30361 aaaccataaa aaagaaagta cagatacccg tagcggatca tcaatgcttt taaagaaatt
    30421 cgttcgcggt cttcttccac caaacggata cgcacaattt ttttgccaag tgtccatcca
    30481 ttggttataa acggtacaat cataaagtat acccctactt ctaataaaag ccaccagata
    30541 tgagaagtaa ctgcccctga atcgaacgca attttaaaga aaatcgccgc taccaaatta
    30601 ataagagata aaaatgtcca gtcgatcaaa taagcgacca agcgtcgagt caatgagaca
    30661 gtctttcctt tttcataaga aagttcatcc attcgtcttc ttgtaggaag cataaatgta
    30721 aataacggcg taacgacaaa accaatgatt ccacccaatg tatttgtcac taaatcattc
    30781 acatcaaata agcggtatgg tcttgggtag ataaaataaa gtccacttag ctgcgtcaat
    30841 tcaaaaaata gagaaaggca gaaactagca atcatcgttt tcataaaaga aagccggaaa
    30901 taatatctta aataaatgcc aaatggtatg agtaataaaa cattaaaagc tggttccaaa
    30961 aatacactct gcttcattgc cggcaggtag gtactaggtt cagacaagac caataccgtg
    31021 ttgtctctga aatgttgcca tgaagcaaat aaatgcagct ccatctttgg ccctgtcatt
    31081 tgggcaaccg tctcctttgc cggaagtggt aagatcacca aaaaatacgc acacaataaa
    31141 taaaaaacga aagaatacaa aataactgcc cttgttacta gaaatgttcc atactttcga
    31201 tattcataaa taaaaaaaac agatgagatc gctaaggcta aaaatggaaa aacaatcagc
    31261 gcaaaacgaa tcggttcgat ataacttgcc atatgactcc tccttgattc tttgtatctc
    31321 tcttatctta gcagtccttt gctgtttcaa gcaagtaacg aggctattat ttctcatttc
    31381 tttcaaagta aagtaagata aacacaaagg gggtgtcgag atgctgaata tgtactttgt
    31441 ttttggcgta cctatttttt tactgttcct ttatgctacg atcgcttatg taagaaaaag
    31501 aactacaatt cattatttag gatttattct cctgattatt tcgggtttca tgctagtctt
    31561 caatcttcag acttggcagc aagcacttct tgaaatggat aaaatgactc cacatgcttt
    31621 gtctaaagtg cttggctacc ctgtctatct gatttggctt cccattttca tttctggttg
    31681 cttggtttta ttaaatattt atcgcggcgt acgccgaata gttcagttac gcaaatcaaa
    31741 gtaaaagaca gaaatcggtt gagcccgatt tctgtctttt actttgctat ttcatcgtag
    31801 acaaaacgac atttacgtga tcaaaattaa cgagtacttt tgacatttct tccacgtact
    31861 cctcatttaa ataaaatatt ttgtttggca gagaagtgca gttctaataa aattcgaatg
    31921 ttttagctat ctacgtttta aatagagata aatcgggata ccagcaagtg tcaatactat
    31981 ccccatcgaa gctaaaaagg tctgagtgat aagcgtgctt gatacgataa aagtccctcc
    32041 aatgattgct actagtggaa taaacggata cattggaact ttgtatggtc gaaacaaatc
    32101 aggttctttt ttacgaagaa gaatgacacc aacaaagacc atcgtataaa aaatccaaat
    32161 gacaaagatc agcatatccg ttagtgtatc aaaaccaccg atcatcatca tgccaatagc
    32221 gatgactagc tctaagatac ctgcggctac aggaacttta gttttatcat gaagtttagc
    32281 aaagagcttg ctaaaaggca ggttgttttc ttttgccata acataaggga gacgcattcc
    32341 agtaagggta tagccattga tggttccata aacagaaatc aagataccaa ctgtgaccag
    32401 tcttccaccg aagccgccaa aaatcatttt tgcaacgtca cttgccgcat tgctgtttcc
    32461 tgcgactccg tcgatagatg cggttcttaa gaacaccgca tttactaata aataaacgat
    32521 catgattccg atgattccta aagaaattgc ttttggcaaa tctttcgccg gctttttcaa
    32581 ttctcccgaa atatttccta cgtgtatcca accgtcgtat gcgaacatcg ttgctagcaa
    32641 gcccgctccc aaggctgaga aaaatgaaag attttctcct gcttggatcg gaaacagttg
    32701 gaagtctact ccttcttgtc ggaacaaacc gaaaatgacg atcacaaaca aaggaataag
    32761 cttgcagaca agcgtaatcg actgaaaagc ccctccagct ttagaaccta aaaaattgat
    32821 caacaggatc gatacagcag ctgtaacagc tacgggaaca atcattgact gggaaagacc
    32881 aaatagattg acgaactgtg taccgaaaat gatcgacaag gctgctacat ttgctggaaa
    32941 ataaatcacg acttgcgccc agcccaataa aaaagctgct gtatttccgt aaatccgttc
    33001 gatatattta atcatccctc ctgtttctgg aatagcagct gccagttcag ctcctgttag
    33061 tcctgcacag acagaaatca taccaccgag aaaccaagaa aacatatgca aactggcgga
    33121 tcctgtaact tctgctacgc ttgcggcttt aaaaaatacc cctgcaccga tcactgtgcc
    33181 cataacagtc gataaagcag taaaaaatcc catcgtacgt tttaattcat tttctatttt
    33241 tacttctttt actggcgtgg cttgatattc ttccatccct tgtccccttt cataccatag
    33301 ccaatcccaa aagtataaaa aactttttgg atgtggttct tgttttatac cgtctctacc
    33361 aatttttctt tcagtttttc caacatatcc tttgtcatca catctaagtc gtatttcggc
    33421 gaaaagcccc attcctcttg cgcacaagaa acatctagtg aatttggcca agattctgct
    33481 attgactgac gtacaggatc cacatcataa tccatttcaa aatctggtat gattttttga
    33541 atagatgctt tgatctcttc cgggtcaaaa gacatcgccg atatattgaa ggcatttcga
    33601 tgaaccaatc gatcaggatc tgcattcatg agttttataa tggcatcgat cgcatctggc
    33661 atatacatca tatccattga tgtaccttga tcaatgaaac tagtgtactt tccttctttg
    33721 attgctgaat aataaatatc gactgcgtag tctgtcgtac cgcctcctgg caaggttgca
    33781 taagaaatca atccagggaa acggacacca cgagtatcta cgccatattt cacataataa
    33841 taatcacata gtagttctcc agctaccttc gttaccccat acatggttgt aggacgctga
    33901 agagtatctt gtggggtatg atcttttgga gtatttggac caaacgcgcc gattgaactt
    33961 ggcgtaaaaa atttcagctg gaattctcta gcgacttcca atgcattgac cagcccagtc
    34021 acgttcaaat tccaggctaa ttgtggtttt gcttctgcaa cagctgacaa aagagcagcc
    34081 aaatgaatca atgtgtccac ttgatattgt tctgctatgc tgcgcatttt ttcgtaatcc
    34141 attacatcta ggatctcaaa gatatcgttt ttctcaatga cttcaggcat gcgtatatct
    34201 gtcgcaatga cattttcact gcctagttct tgacgtagcc gtgtgatcaa ctctgaacca
    34261 atttgtccta aacatcctgt caccaatact tttttcatga tgatcctccc tgatcttttt
    34321 tactactatc ttcagatgat taacaatata tgcgtgaaca tgttctctaa ttttaaatag
    34381 acgaaaaaac taaatttagt taaataattt ccatttcttt tcctattttt tgataaactg
    34441 ctactgcttc atcaagcatt tccctcgtat gttccgcagt cggcatattt ctgatcctgc
    34501 ctgtcccaag aggcacagtt ggataaacga ttggtttagc ataaatccca tgttcgatca
    34561 gcttcttaga gaaagtctga gtcacttttt cgtctcccaa aatgactgga gtaataggtg
    34621 tttcagacat accgatgtta tagcctactt ttttcaattc ttctttaaaa taattagcat
    34681 tttcccaaac tttttcgaca agttccggat gttcttgcat caaagtaatc gaagccaatg
    34741 cagctgcagc tgcacctgga gttagcgaag tagaaaatag gaatggccga cttctagctt
    34801 tcaaccagtc gattagtgtt ttcgatccag ctacataacc cccaacaaca cctatagctt
    34861 tcgataacgt gcccatttgc atatcgattt tgtcgcttaa tccaaaatgc ttgacggttc
    34921 ctgcaccttt tccagttacg cctgagccgt gcgcatcatc gacgtaggtg atcagtccat
    34981 atttttcaac aatcggaata atttccggca atcgtgcaat gtctccatcc atggagaaaa
    35041 caccatctgt gatatacatg atttttttat atttcttact ttctatcgct tcctttgctt
    35101 ttttctcaag gtctttcata tcttgatgtt taattcggat gatttttgct ccggataagc
    35161 ggcaaccgtc aataatagat gcatggttta attcatccga taagatagca tcttcttttg
    35221 tcattactgc agagatagcg cccatattgc agttgaatcc agattgaaag gcgattgctg
    35281 cttctgtccc tttgaattct gcgatttttt cttctaattg ctgatggatt ttcagcgaac
    35341 cattgatcgt tcggactgct cctgctccta caccataagt ttcggttgct tcaatacatg
    35401 cttttttcaa ttcttcgcga ttggcaaatc caagataatt gttggatgct agattgatca
    35461 tcttttttcc gtctacgatg atacaagcac cattttcact ttctagtatg tcgatcgtat
    35521 tgtataaccc cttttctttc agttgatcca attctttctc taaatacatt gttagttctt
    35581 tggaagacat tgtctccacc tccatattca tctgtcttgc agaacatacc aaacatatat
    35641 cgtgtcattc atctacaaag caagtatgct atatttttga taacgcttac actgattatg
    35701 agaattattt aaccacattt tttatttaca gtatcttttt actttcaaat gacttatata
    35761 gtatgaacaa ttacctttta gataatgatc tattttactc tacctgggca gataaataca
    35821 acacaaccat tgatagaagc cctttcgtta aaatcaattg tattctgaat atttaattta
    35881 tttattgcat agaccaataa aaatacataa aatttccatc ttcgtttatc agcaattttt
    35941 aattttactc tctgaatatt tcttaaaagt tttacttgtt gaattc
//
LOCUS       DACEKG010000023        35663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEKG010000023 DACEKG010000000
VERSION     DACEKG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.023"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..2200)
                     /locus_tag="IW664_001044"
                     /pseudo
     CDS             complement(1..2200)
                     /locus_tag="IW664_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2187..2405)
                     /locus_tag="IW664_001045"
     CDS             complement(2187..2405)
                     /locus_tag="IW664_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848423.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(2407..3450)
                     /locus_tag="IW664_001046"
     CDS             complement(2407..3450)
                     /locus_tag="IW664_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0848424.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(3605..3931)
                     /locus_tag="IW664_001047"
     CDS             complement(3605..3931)
                     /locus_tag="IW664_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848425.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(3939..4142)
                     /locus_tag="IW664_001048"
     CDS             complement(3939..4142)
                     /locus_tag="IW664_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848426.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            4217..4867
                     /locus_tag="IW664_001049"
     CDS             4217..4867
                     /locus_tag="IW664_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0848427.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4922..6088
                     /locus_tag="IW664_001050"
     CDS             4922..6088
                     /locus_tag="IW664_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0848428.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(6177..6569)
                     /gene="rpsI"
                     /locus_tag="IW664_001051"
     CDS             complement(6177..6569)
                     /gene="rpsI"
                     /locus_tag="IW664_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0848429.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6585..7028)
                     /gene="rplM"
                     /locus_tag="IW664_001052"
     CDS             complement(6585..7028)
                     /gene="rplM"
                     /locus_tag="IW664_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0848430.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7547..8014)
                     /locus_tag="IW664_001053"
     CDS             complement(7547..8014)
                     /locus_tag="IW664_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0848431.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8134..9672)
                     /locus_tag="IW664_001054"
     CDS             complement(8134..9672)
                     /locus_tag="IW664_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0848432.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9752..10438
                     /locus_tag="IW664_001055"
     CDS             9752..10438
                     /locus_tag="IW664_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0848433.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10639..11166)
                     /gene="msrA"
                     /locus_tag="IW664_001056"
     CDS             complement(10639..11166)
                     /gene="msrA"
                     /locus_tag="IW664_001056"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0848434.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11250..11927)
                     /gene="pgmB"
                     /locus_tag="IW664_001057"
     CDS             complement(11250..11927)
                     /gene="pgmB"
                     /locus_tag="IW664_001057"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0848435.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11924..13054)
                     /locus_tag="IW664_001058"
     CDS             complement(11924..13054)
                     /locus_tag="IW664_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0848436.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13054..15237)
                     /locus_tag="IW664_001059"
     CDS             complement(13054..15237)
                     /locus_tag="IW664_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0848437.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15234..16259)
                     /locus_tag="IW664_001060"
     CDS             complement(15234..16259)
                     /locus_tag="IW664_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0848438.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16304..17131)
                     /locus_tag="IW664_001061"
     CDS             complement(16304..17131)
                     /locus_tag="IW664_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0848439.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17128..18195)
                     /locus_tag="IW664_001062"
     CDS             complement(17128..18195)
                     /locus_tag="IW664_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0848440.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18221..19057)
                     /locus_tag="IW664_001063"
     CDS             complement(18221..19057)
                     /locus_tag="IW664_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0848441.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19054..19923)
                     /locus_tag="IW664_001064"
     CDS             complement(19054..19923)
                     /locus_tag="IW664_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0848442.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19993..21276)
                     /locus_tag="IW664_001065"
     CDS             complement(19993..21276)
                     /locus_tag="IW664_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848443.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21290..22975)
                     /locus_tag="IW664_001066"
     CDS             complement(21290..22975)
                     /locus_tag="IW664_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0848444.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23250..24275
                     /locus_tag="IW664_001067"
     CDS             23250..24275
                     /locus_tag="IW664_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0848445.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24870..28523)
                     /gene="rpoC"
                     /locus_tag="IW664_001068"
     CDS             complement(24870..28523)
                     /gene="rpoC"
                     /locus_tag="IW664_001068"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0848446.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(28602..32219)
                     /gene="rpoB"
                     /locus_tag="IW664_001069"
     CDS             complement(28602..32219)
                     /gene="rpoB"
                     /locus_tag="IW664_001069"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0848447.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32947..33915
                     /locus_tag="IW664_001070"
     CDS             32947..33915
                     /locus_tag="IW664_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848448.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33933..34838
                     /locus_tag="IW664_001071"
     CDS             33933..34838
                     /locus_tag="IW664_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848449.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34835..35641
                     /locus_tag="IW664_001072"
     CDS             34835..35641
                     /locus_tag="IW664_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0848450.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        10110 a         7531 c         6435 g        11587 t
ORIGIN      
        1 cttgtcccac tactttgtac attttctcgt acatatactg ttcgatttcc attttgacta
       61 cttgtagtat tttgtaagtt ttctcgaaca tctactgagc gcctatcttg actacttgta
      121 gaattttgta gattttcttt gactgatttt tctaatccgg tatttgaatt tttagcttct
      181 tggattcttt ctttagtcaa cgcttctttt tcgtccatat tagcgcctgt acgcccttct
      241 acacgtttcc ttaacatttt tcctcacgat tcattgcttc aggcgtgtat tttgcttcct
      301 gtcgcttctg attaattaat acttcagcag acttagcttg accgtgcata gcatttttac
      361 gttcttcaat atcttgttta actttttgct gaattgcact tgcaggatta atatttgatt
      421 cagcattatt tttcattgct tcacgcatac gttcagaact agacataatt ctatcagcgt
      481 tttcaacttt agcgccatct tttctatccg aaagacgtgt tggcatagaa tcagcatatt
      541 ttccagtttc atcttctttg tttttagcta ctataggttt gctattttta ttaatagcgt
      601 acacggtacc atcatctaaa ccttctttga atttattctt ggtatcatta gccatatcac
      661 gcatactctt aaatggtttt gttgctcttt cactggccat tgtagctcca tccgaagcta
      721 agcctgataa tcctcgttca cgagcatacc caagtgtttt ccccgtttta ttagcttttt
      781 gagaaagtga ttgaccaaag ttgcgttcag gttttcgttc ttccccttcc gcttgatcgt
      841 ttccaccact atttttccca cctttagatc ggttcatacc acctgttcct ttacgaaaca
      901 gcatagctcc catgccaaat gctgaagcaa gctggccata gccttctttt aaaccaatat
      961 ccacgccaaa gtaacgcatg actgttccac taccctttat cagcacaata acagcgcaga
     1021 cgtacgctac tgcttttata aaaggattta cagaagaacc gctacctgtt ccgagataat
     1081 taacaaacat tgcaaacata gacaatccga acccttgaaa tccgactgtc aaataacact
     1141 gtaaaatatc tgacaaaact tgctttgatc gttggcctgt ttccaagtca gtagcaaata
     1201 ctaaaactcc aagtacgcgt ttaaacgcta gctcgagtat tgctgtaatt ataatgaaag
     1261 cactaaaaac atacgctacc gctaaagcaa tcaatccaaa agaaatcccc catagatttg
     1321 cttgatagcg ataatatcct gatttaatgc cctcaacaaa ggtcgacaga aaattatccg
     1381 aaaatttggt agcaacaaat tcattatcag aattttcaac taattcataa cgcagattat
     1441 cagctattgg gttctctttt tccattttgt cgagtacatt atctgtcaat acttgcgtaa
     1501 gatccgtttt ccaaaaattt tcttttgtta gattgttccg gtcattcgtt ttatcgatca
     1561 tggaatattg atctgtttta ttgatataag ctaaatcagt aaccccttgt tcaatcagcg
     1621 accacgccac gccttcgtcc gaatttgtaa tcgtagtcgt atcattataa aatgttttag
     1681 aaaactctat tccgctgtga atcaacgttg gcattaaaag aatcaatgaa attgacatta
     1741 caacattcat tccaacagat tttagatcaa ccgttccctt acctgtaatc attttataac
     1801 caataatcac tagtgaaagt atcattaagc caacaacaat ggtacttaaa acagcttgat
     1861 agactttgtt caatccggta ctttctacaa agctaaacaa atctaaaact tcgggaatca
     1921 tattttgaat gccatcgaca acagcgaaca ttgattttat aacagaccag cctatccaca
     1981 taccgaactg gctgtaaaaa tttccgttgt attttttcaa ataattctgg taaagcggat
     2041 tagttaaaaa ctcagtttga ctctcagtat catcaaacca attgaaaata ctgccttctg
     2101 caatgttact cgtaaaaaga atcacagcta ttagaacaag aaaaataacc ggtaaccaat
     2161 ttgtttttct attagcaatc ttttttttaa ttattttcat tgttacctcc ctcagcccat
     2221 tttcttaggc tctcagagag tttctgagct atttttctgt caattatgat atgagttacg
     2281 taatcatcat taacagcttt taaatcgaga acaatagtat ttgagcagat actctcggtt
     2341 aactcaagag tatctgcgtt ttgatcaaga cagtgaatct tatctactag atgattttca
     2401 atcattttaa attccctctt ttttttgtag cttgattagt tctaacgctt tgttttttag
     2461 agaagtaaag atataattat ttttttgttt gatacgatga tctgtttttg ctcttcgtat
     2521 caagtcacgt aaagtcattg ctagctgttc tctaacatga tctacaaaat aactattatt
     2581 acaaatttca tagataattt tcagttgaac tttttgttct ttttcagcag atttaattgc
     2641 ataattgaat tttttgtacc agttgtcgaa ttgacgataa tcactattgc tagccagttt
     2701 taagcaattc acaatacttt ctccccacat ttgtttaaga agattttctt caataaatgt
     2761 ttcgatcaat tcttcatcta atttattatt tttagttgaa agtgattcaa aaagatcatc
     2821 attatgtgat tctttaatat ctttactatc tttataagac ttatataatt ctagttcatt
     2881 ttttgaacta tcagcaaaat tgtttttgtc tcttgttttc attgatgtgt cgcatagttc
     2941 aaattttgaa ctatcaacag aatattgatt tgttccatag ttcaaatttt gaactatcaa
     3001 gtttttttga ctattttctt gatgggttat cttattaatt ttataaacat catctttgca
     3061 cagtacaggc tttaacaaat aataaaaatt tgggaacatt tgcattttat ttttattgaa
     3121 gccatttttt cttttcacaa gaagtttaac tttttgaagt tcatctagtg ctgataagat
     3181 tgtatttttt gacattcccg aaatttctac gagatttttg atttcaaata tcaaaaaaat
     3241 tttttgattc tcatcataca agttattttt caatgaaagt ctatttctat cttttagtaa
     3301 catataaact aacttagaac ttagtttcat tttcttatat ttttcattta cgtttaaaaa
     3361 aataggatat tgatagtata attctgcata tatatcagaa ttagaatagc cttgcttttc
     3421 aactaagctt tcaacttctt tttcattcat tgtcacacct gcttctgatt ttatttgcta
     3481 taatagattc aaataaagtt ttctcagaac tttattattg agtgttcccc cgatctgcaa
     3541 actgacggga acactctttt taattttttc tacttcatca ttattcaaaa aaacacctcg
     3601 ttttttaaat ttgatgactc aatatccatt ttttgcattc ttcttttgaa aacaactcca
     3661 ttttttctaa taaaataact ggcaatccca ttcttttaaa tttcaaaaaa gttgtatatg
     3721 atacattcaa atatttagcc atctcttttc cttttagaaa tggtctttct aacccggtat
     3781 caattcttgc ttgatcaata gcctcttttg tcatttcaaa aacataattt ttcaattgat
     3841 tcgtttgttg tttatctaac attactgtaa ttcctgcatc cattattttt ctcctctcat
     3901 tttctatttc ttcaatcagc caactcgcca agttgtattt atttagattt caaatcatta
     3961 agtaaccaat ttaccaactt ttgataagta gatgctctaa tactttcttt gctcccattt
     4021 tcgattttga catatgtttt ccgatttata cctatcaatt ttgctgcatc gtttttagta
     4081 agtataagat cggctctatg tcttcttaag cttctaatga attgatcatt aagagttatc
     4141 attcagcgcc tccttttaaa ttattcactt tgtggttttt atcttaataa gcacatttta
     4201 aaccacgaaa cgagtaatgt caattaaaac attactcaat ttgtggtttt tatgctataa
     4261 tagtttggaa tttactatag ggggtgttac tttgaataga ataaaatttt atattgattc
     4321 cagtaaaaag accattaaac aaattagtac agaaactggt attaactata caactcttac
     4381 gaactattac cgtggtgagc gaaatcctag aaatactgaa acatggaata tccttgctga
     4441 ttatttccat gttcctgtaa gttatttgat gggattagac gaaacaaatt ccatagaaca
     4501 acctaatctt gatagaataa aaactgctcg tttagaaaaa aaggacaaaa tagtcaagaa
     4561 tattgccagt cttgactcag attattttac tgaattatct aatctatact tttccatgac
     4621 ttctttactt gaagtcaaac ttcaagaaga tgactccgac tttctttttt taaaaagtat
     4681 tctagaggac tcctttgaat acacgattct atttgccaaa caaaagaagt ttattaataa
     4741 agctttaaac ttaaatatcg atgaaagaaa agagttagaa tctgagttat ttggcaactt
     4801 aacccaagta caggttaaca atctaaaaag agagataaca gctcgattaa tatcaaaact
     4861 tcaataaaac taattattca atcagccaac tcgccaagtt atcagattgg agagattagt
     4921 tatgtcaata caaaagtaca caacaaaaag cggtgcacga tatagtgtcc gcttgtatgt
     4981 tcgtaaagat gaaaatggaa agcaagaata ctatgtaaaa aaagggttta aaactgaaaa
     5041 agaagccaag ttacacgaag caagaaaaaa ggtcgagatt gaagataccg gctatcataa
     5101 gccttctaat gacttgttta taacagtgta tgaagaatgg ttgccttctt ataggaatcg
     5161 tgtacaagaa acgacatatc aacgtacaaa agaccttttc cgcctgcata tactaccaaa
     5221 attcgggaat aagtccattt ctaagattac tcctgttcac tgtcagaatg cagtcaatga
     5281 atgggcttta aaattcaaga atatcaaaca gcttaagtct tacagctctc aaatatttga
     5341 gtatgccatt tttgcagagc tattacaccg aaatcctatg gcaaatacaa atctaccaaa
     5401 gcgggaaaag aaagaaaatg aaaattactt ttcattagaa gaactaaaag ctttttggga
     5461 tattctcaat aaagaagagc ctttaaaaca tattttaatc ttccagcttt tgattactac
     5521 tggtatacgt aaaggagagc tagctgctct tcattggtct gacatttatt ttgaagaaca
     5581 gctaatgtat gttagaaaga gctatgcaac tattcgaaat gaagagaaga atgcaagaag
     5641 aaaaactgtc aggattcaaa aaaatacaaa aaatatatct tccgaacgaa tattaccaat
     5701 tgatgcacaa actattgaaa tgttaaaaat ctggaaaaaa gaacaagctc gagagctttt
     5761 gcagtttggc ataaatacaa atacaaaaaa tcaacttatc tttacctatg taaatgcaga
     5821 cggtgacatt aatcaacctt tgcatgcgga ctattcaaat aatatattga aacgtttaga
     5881 gaagaaatat aaatttaagc acgttactat tcatggattc cggcacactc acgctactct
     5941 tctactagaa ggtggagcaa gtatcaaaga aacacaagat cgattgggtc ataaaaatgc
     6001 tgaaactacc cttaacactt attctcatgt tactgaaaaa gctcaacgaa atgcgttaga
     6061 aaaattcatt gcttacactg gtttataatt ttgtcattac ctttctcatt accagaacga
     6121 aaaaaagaca ccttctcgca atgagaaagt gtctgaaatc gttggtataa caacgattaa
     6181 cgttttgaaa actgtgaagc tttacgtgct ttcttaagac ctggtttttt acgttcgacc
     6241 atacgtgcgt cacgagtaag taagccagca cgtttaagag ctaaacggaa gtcaggatca
     6301 acttctagca atgcacgagc gataccatga cggatagctc ctgattgtcc agcgtatcct
     6361 ccgccgttta cgttaacaaa tacgtcatat gcgccttttg tttcagtaac agcgaaaggt
     6421 tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt cttcaacatc ttttttattt
     6481 acagtaattt tgccagttcc tggtactaga cgtacgcgag caactgcgtt tttacgacgg
     6541 cctgtgccga tatattgaac ttgtgccaat gaaattccct cctattagat taagtttgtg
     6601 atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt ctgctccacc gtatacgttc
     6661 aattttgtaa attgtttgcg gcctaaagtg tttttaggaa gcataccttt tacagaagtt
     6721 tcgatcaaac gacgagaatt tttagcacgc aattcgccag cagtgattga tttcaatcct
     6781 cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg cttttttacc tgttaatttc
     6841 actttgtctg cattgatgac gatgacgaaa tcgccagtat ccaaatgagg tgtgaaagtt
     6901 ggtttatttt taccacgtag tacagatgca acaactgttg aaagacgtcc caatggaaca
     6961 tcagttgcgt ctactacata ccatttacgt tctacttcgc ctggtttggc catatatgtt
     7021 gtacgcacgt tattttcctc caatttaagt tctgatgttt gacatacact tcgagtttcc
     7081 ggggctcttt gtggggcaaa caataccatt taacatatta cccgctaatc tagtcaatgt
     7141 caacgttttt ctgcttgatt gaaaaattta tatgaaactt tacttctttt ctttctttta
     7201 tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag aggctgtgac aaaagtcttg
     7261 tcacaacccc ttattttgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
     7321 aatatccata aaacataaga agctgttttt taaaattgtt tcttgtgact cggagctgaa
     7381 cggcaggctt acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
     7441 aaaaaacggc aaaatatgag gaactatttt gacgtttgtt tctctaatac tcaggtcaaa
     7501 acgctgattt cacaacctct ttttcggtca agttcaacaa cagtttttac gcgtcgatct
     7561 ctggcgcttt gcccaaatga gcttggatca tccagatacg tttttctgtg gctgttttga
     7621 attcgataaa gatatcttgt gttggatcgt cgccttcttc tccagaaact tcgattcctt
     7681 tttggtaaag atctgctaga taacgatagc ctgcaacaag tgtttccaaa cgttctggaa
     7741 tagtacgatc ccagcggcca atttcatcag ggatttttgt gttatctgca aattcacgca
     7801 atgtagaata aggtgaaccg tctaatgtga tcaagcgttc ggatacttca tctaactgag
     7861 cgttcaaatc atccatgaac tcatccatca ttgggtgcaa tgtaaggaaa cctggtcctc
     7921 tcatgtacca atgtgtttgg tgaacaacaa cagacatttg gcttagatct gcaactaatt
     7981 gatttaatac ttctttggtt tgttggaatt tcatctttct tgcctcctaa cattttctta
     8041 cacttaaaat aatagtagac tttcaatgaa aagtacaaca aaatcattta cctgagaacg
     8101 attttcagtt cgctttttta tccgtaaagc caatcatttt tcagaatcag ctaattcttc
     8161 tcgcagttga gcaaattgct gataagtctg tgctaaatgc ttttgtgtcg aaacaaataa
     8221 tggatagatt ttctgatatt ttttatgatc agtttcttgt actccaactt gctctttcaa
     8281 ttctatcaat tcctgtgctt cactgatact ttggatcttt ccgatgcttc tccagcctaa
     8341 aatcacagct cctaatgcgg atgcttctgt gacagtaggg aaaacaagcg ggtaacccag
     8401 tacatcagcc atcatctgac gaaaaatcgg agattgagca aacccgcctg tagcacggat
     8461 ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc cgataaaggt tgagacagat
     8521 cccttctaga atcgccttca tcatttcttt ttctgtatgt tgccgctgta aaccgataaa
     8581 actgccaaac gcatctgcgt cccaaagcgg tgctcgctca ccaagtaaat agggatgaaa
     8641 aatcagacca ttggcacccg ctggcacaaa agcgatagcc gaaagcagtg catcatatgg
     8701 attttctcct ttttctttag cagattgaac gacttcctgt aaaaatgtat gccctgccca
     8761 atcaaagatg ccagcaccat tgctagtcgc tcctcccact acccaatgtt tctgatccag
     8821 aacataacaa aacgtctcca tttctggatg gaactgaggc tgagtagtaa taaaacgaag
     8881 tgctccgctc gttcctacgg tcaaagcggc gatcgtatca ccaaaagcac ctaaccctag
     8941 attagaaagc ggcccgtctg cccctcccaa aacaaatgat atatcatttg gcaaacctag
     9001 ttctgctgct gtttttgatg gtagacctgt tacttgatag gtggaaggaa agacttgcgg
     9061 caattgctct attcgaatgt tcaagtagtc aagcacttct tctgcccatt ggaattcatg
     9121 gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg ctgatcagtt cgccaaacaa
     9181 tctataaaag atatagtctt tgataccgac aaaataagca gtttttttga ataattcagg
     9241 ttggctgttt tgcagccact tgattttgtt caatggtgtc attggatgaa tcggcgtccc
     9301 agtctgccga taaaattctt tgccaagcac ggtatatttc agtttttctg cttcgtccgc
     9361 tgctcggtta tctgcccaag tgatcattcg agataacggt gttttctctt catccaacaa
     9421 tatcaggcca tgcatcgcag aggagaagga gacaaattga atgattgtgt ctgaaggcaa
     9481 ctgtttaatt acttctttta taacggtgca gacaccttga aagatcaatt cctgatcttg
     9541 ttctgccatc ccttgtgttt cttggatcaa agggtagcga aaataagcgg atgctgcttc
     9601 ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg gttccgatat ctaatccgat
     9661 tgaaacgttc attttttcac ctcaaatttt atgtatgtac aaaaatttag tttaagcttg
     9721 cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt tattttatca ttggttgctg
     9781 tatcggatca tttttatgtt taaccgccca gcggattccg ttaggtcact ctattattta
     9841 tcctagatct cattgtgtca aatgctgtcg ttcgctttct tggtatgaat tgattccgat
     9901 tctctcgatc atcgtccaac gatttcgctg ccgctattgc aggtgtcggt tgcccttcta
     9961 ctatttgttg gcagaagcct tatgtggtgg tctttttgcc tggttcttta ctttttcttc
    10021 cgcaagaaat ttttcgacgt tcatatggat gctttcggct ttgctatttt cgttgatgga
    10081 tttattttac ttcatggtcc attctcacac gctattctgt tcttgggcaa ttttatggat
    10141 tttttggttg caggccggcg tcttccaatg gcaaagtgtg ctgttaacgt ttatcgtagg
    10201 cggtgtgtgc cttcgctatg gtcaatccta tctcggagct ggcgatactc tgttgatgct
    10261 ttcttggagt ggtggcttgt ctctagaaga gttgcttcag atcttgtttc ttgctagtct
    10321 gtttggaata tccgtttttt ctgtttactt tatctgtcat aaaaaaaagt tggaaaagct
    10381 gcctttcatt ccttttttga gcagtgcgtt gctgattgtc cttcatctta ggtattgaaa
    10441 aaaggttgtg acaaacgact tgtcacaacc tctgatatat agtgttctct taccaaatcc
    10501 ttgtgacttg gagctaggcg acaggttcac aacctctcca ctaaatggtg ttcaatcagc
    10561 tgaccctcag tattagctca aaatgctggt tttacaacct ctgatatacg gtgttccata
    10621 agtagctttt tcggcttctt atttcttgac gccccaatgt cgacgaagga aacctgcacg
    10681 tccagaacct tctcgataaa gattgtaatg gagcttgttt ttcttgtaat aatcctgatg
    10741 gtaatcttct gcaggataaa attcgcttct tggttcgatt tttgtcacga ttggcttatc
    10801 gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct gcaatatttc gttcttcttc
    10861 atcattatag tagatcactg gacgataaga atcaccgcga tcagcgaact gtcctaatgc
    10921 gtcagtcgga tcggtttgct gccaatagat ttccaccaac tgatcataag aaataacttc
    10981 tggatcatat tcgatctcta ctgcttctgt atgccctgtt gtgcctgttg ttacttgttc
    11041 ataagttgga tttggtacat gcccgccagt ataaccggat gtcacagaaa tgatccctgg
    11101 ctgctcttca aatggtttga ccatacacca aaagcatccg cctgcgaaaa ctgctttttt
    11161 tgtcatataa aatccccctt ttttctcatc ataccttgat ttccaaaagg aggataacgt
    11221 tttgcttgtg acagaaaaaa cggaaactat tatatacgtg atacggcttc tgataaatgc
    11281 caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa tcacatattc agcagaagga
    11341 agatttatgt tttcacctat tcccaataca tgcatgttac cagccaaagc tgcttgacaa
    11401 ccagcttctg aatcttcaat gaccaagcat gcatttggcg gtacattcaa ttgctgtgct
    11461 cctttgagaa aaacttctgg atcaggtttt gcttttgata cttgcgtgcc gtcaataagg
    11521 acatcaaaat aacttgtcaa attcgttttt tctaaaatga ggcgtgcatt tttgcttgct
    11581 gatcccaatc cgatcttgat gtttgttttt ttcaagtact caagaacagc tattgcacca
    11641 ggcaaaacgg atgtttcatc cattttttga atcgcttgaa gatatagatt gtttttttgc
    11701 tcagcaaagg ctgatttctc tttttctgag aatttctgtt ccatttttcc aaatgacagc
    11761 aatcgctcta gtgactcggt tctggatatt cctttcagtt gctcgttttc tttctcagtg
    11821 aatggaatgg acagctggtt agctaaaacc agccacgctt cgtaatgata acgagctgtg
    11881 tctacaagaa cgccatctaa atcgaatagt gctgctttga ttttcatcga ttacctcctt
    11941 gaaaatgaac gacttgaagt aaagatgcga tgtttttagc tgtgcgatga agattatcag
    12001 tggcttgctg taaagcttct tgtaaaggca cagctgcagg aaggatcgga aaaacggccg
    12061 aaatgccaac atctgctgct ttttcgcttc ctttaccaac acttccgcaa atagctatca
    12121 caggaacgcc ctctggtact cttctagcaa tcccaactgg cgcttttcct tttagacttt
    12181 gtccatctag acgtccttcg cctacgatta cgaggtctgc ttgctgtacc cgtttatcga
    12241 actccagtaa atccaatacg tagtcgatgc cagagactat tttccctgaa gcaaatgcta
    12301 acagtcctgc acccattcca ccaccagcac tactgcccga tgcctcgatt acttgagggg
    12361 caaatctcat ataaaaatca tgcatggctt ggtcagccac tgctaattca ttttctttca
    12421 agcctttttg tggtccgaaa acaaaagaag cgccttcttt tccgcataaa agattagtaa
    12481 catcagtaat gacagtgatt ttggctttaa tcagctcaat aggcacgttc gttttatcaa
    12541 ctgtagcaat tttacctaga ttttctccca gtgcttctac tttttgatta tcttgatcgt
    12601 aaaaagcata acctagacct tctgccatgc cgatgccgcc atcgttggta ctacttccgc
    12661 caacgccgat gatgatgtgt cgaacacctt gttgcaacaa ttgtcgtatc agttctccta
    12721 ctcctcgcgt ggataaccta agtggctgtt gttggtttag tgggacatct ttcaatccga
    12781 cgatttctgc catttcaaaa atcgcagttt gaccgtctga agcgtactgg atttctttgt
    12841 ttttgccaaa tgcattagta acgactgctg tttgccgctt caaccttaaa ctgtctgcta
    12901 atgatgaaac cgttccttct cctccatcgc ctataggtag cagatcatat tcagcctgaa
    12961 caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc ctctaaggct gtcagacttt
    13021 ctttgaaaga atctggcgca atcagaatac gcattatttt cccctccgtt ccaagatcag
    13081 ttctttttcc agctggtatt ggtctccatt gacaaaaata ggtaatgttt ctttcgtctc
    13141 tttttttatt actatttgtt tttttgtcaa aacaaatagc aacctgtgat ttttccaaat
    13201 aaaaggaaaa gaaatttttt cccatgctga aggaagtctt ggagaaatag aaagagttcc
    13261 attcgttact gtaatacctg aaaaaccaaa tataactgct agccatagtg ctcctaatga
    13321 agcagcatga attccgtcat ctgatgaatg cggatctggt cctaaatcaa tcaggcacgc
    13381 ttcttggaat aaatgatacg cccattccat ttcttggata cggcaagcct ctatggcgtg
    13441 aatcgcttta ctcaacgatg agtcgtggat tgttttgttt tcatagtaat gcaaattctt
    13501 agaaacgatt ggttgagaaa aatactcagg taataagtat aacagcatga caagatctgc
    13561 ttgcttgctg acttggagtt catttacttc gctacgggca taatcaagta aaatagactg
    13621 gctgccagga cgttctttgt aagtggtcaa atcaataact ggcttactga gatacgtgtc
    13681 atcttgcgga atcagctgtt ctttgttagg cactggcaaa tataacttat ctaaaaaatt
    13741 ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat ttaacagcta aggccacatt
    13801 ataatgcgcc agatagttcg tatatgcatt attgtcgata tattctgtgt attcatctgg
    13861 accaatgact ctgtgtatct ctaaatgatc atcgactttt acagctctgc tcagccaaaa
    13921 agctgctgtt tcatgaatga gcgtttttcc ctttgtttct aaaaacgatt gatcctctgt
    13981 tgcttggaca tactcttgaa cggcataagc aaggtctgca ctgatatgat gttctgcggc
    14041 agctgaagca acttcttgtc ttgttccggt tttgatattc aatgcagcaa atttgggtgt
    14101 ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt gcaccttgat agtgataatc
    14161 tcttgctttt tgccgtgcgc cttctaatcg atcataacgg tacagcagca aattctttgc
    14221 tacttcagga aactgatata aaaagaaagg caggataaaa atttccgtat cccagaaaac
    14281 atgcccttta taaccttcgc cagttagtcc cttcgcccct acactaacac ttggatcatt
    14341 gcggggagtc ataatctgta aatggtaagc agcgaagtcc atcgctaact gatcgaaaga
    14401 agaatcgcct tggatcgtta tccgatgctg atgccaaaaa tctgtccaat aagacatcga
    14461 ctgctctaaa atttctgtat aactaacgct tttctgggct tgggcaatac ttttcttaaa
    14521 cgggtctgtt tgctgaagag aggtagagag cacgatgatc ttctccagcg taaaggacgt
    14581 attttttctg atcttttctg aaaaggaagc ggtgatccgg cgattttttg cttggaatac
    14641 acctgcttta ttgaatttag ctgcaatccc aatatcaatt ttactttctg ttgtctggta
    14701 ggatgcactg atcaaatcgt caccaaatac tcgcagttct ttttcaacca actgctgtgt
    14761 accaaaattg gtttgctgtc catcgatacc cgttttcaca tctacttggc agtcttcggt
    14821 caacggcttg atgaccactt tcagggctgc ctgatggaga ttattttgat cagcaacacg
    14881 acaagtaatg acttccactt tttttccttg agcagtttca atgagcgcat agcgtttaag
    14941 ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga actttcatcg tctcaagaga
    15001 aaaatgttca ccgtcgattt ttatttccca tctgatgaca tctgggagat tcactaactc
    15061 ggctgattct gctcctaaag gtcggttata tacaccagct gcaaacattc cccttacttg
    15121 attccgatag tcttcctcct ggcttcctct gataccaaaa aatccatttc caatggtcat
    15181 ctgactcgca tacttattga tatactctaa atcaaactgt ttttcttcta ctttcataag
    15241 gtaataactt ctcctgtttc tgccgaacga tataaatttt ccaccagatt ctggatgaca
    15301 tatccttgcc agccatctgc aatcatctga gtctggtcac cgagcgcccg attcacaaaa
    15361 tgagccatac ttagttcatg tgggtcatta actggcgtgg tcttttgtaa ggaaaccagc
    15421 tttccatcta catcttcgaa tagttcgcct ggatacagat tagcacctgc ctgatcgcca
    15481 caaaaagcca cattcagttt gtcttctctg atattcaaag caaacgaagt attcagttgg
    15541 atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg catcttctac tgtgaatttg
    15601 cttggatccc atttcccgaa tttccctgaa ttttttttcg taccaatttt ttggaagctg
    15661 tacgcccgaa cgctttttac tttaggaaat ccagtaatat acatcgctgt atcaagcatg
    15721 tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt ctttgtcgat aaacgttccc
    15781 cagcctggaa caccagaccg acgaagcgca ttcgcttcta cgtaataaat atccccgaga
    15841 acatcaagat gattcttgat cattgttgct tcgtcagaaa aacgatgctg gaatccataa
    15901 gccaaaatct gcccgttttt atcggctgtc tctgccatct ttcttgcttg atcagctgtc
    15961 atagctggtg gtttctcgca catcactgaa gcattagctt cgagggcagc caatacatgt
    16021 tcgaagtgaa agcgattggg tgtacagaca ctcacgatat cgggagaaat ctcattcagc
    16081 atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac gtcggcaaaa gtcttctgct
    16141 cgctggctga ctggatcaac cacagcggcc aattctaaga catctgatct tttttgataa
    16201 taagcagcat gtactttttc tgctacttgt ccagcgccta tgattgctac tttcttcatt
    16261 tatttctccc tcctttgctt atttacttat tgttattctt tctctattct ttctctaaat
    16321 catttaaatg acgcgctgtt tccagacagt ttttcaagta ttgcaatgat tcctgataaa
    16381 ttttcacttc attttctcct cgcaaacgac attcatatac catataacct tcatactcat
    16441 tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc gccagatcct ggttggtagc
    16501 gatgattatc tgctaaatgg atatgaccca catactcttt ttgcgtagtc aatgcttttg
    16561 aaatatcatc ttcttcaata ttcatatgat aaaaatcagc gatgatctgc acatgtttca
    16621 attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa attgatcata tggtcttggt
    16681 atcggttcaa aggctccaaa taaataaccg tacctgtttc ttcggctact ttatctaaat
    16741 aacgaagtga ttcgctgact gctttatagt ctccttcttt gcttcggggt gaaaccatcg
    16801 gcggtaatcg ataagtgaac attccccaag ctgctgggat aacgattcct ttgcctccaa
    16861 cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac accattaaga cgccgttctt
    16921 cgataaagtc accgatccac ccatcgtagc cgttacaagc tgtcactact gggatccctg
    16981 tttctttgat tgcctgtttg actgtgtcga tattttctat taacaactta ccgtcgattt
    17041 cgtattcttc aaatccacag tctttgataa atctaaattt ttctaaaatg tctcttggga
    17101 aaaatgcttg atcttgcgta ccgaatttca ttgttctcct cctcggactt gaacgcccat
    17161 tttgatactt tcttctgggt gctggtctac ataacgcatg taagcttctg cgctttctgc
    17221 aaatggcaca actggatcaa tgatttcttc acaatccaag taaccgttca tcaataattc
    17281 ccaacatgtg tcctcgattc gtttacggtt ccatcttggg taatctgggt ttggttcact
    17341 agatgctcga gcaaagacga tttttgcatt attgaaatgt gcttcgcgac caagattaaa
    17401 cccagctgga aaaggtttag caaaggcaac ataagaaatg atcccgccat agccgatccc
    17461 tcttaaagaa gcttgtaatg cggcagcact gccactcgtt tcaataactg aatctactcc
    17521 acgtttatct gtcaattcct ttagacgaaa accgacatct gctttcatag gatctagcgt
    17581 gtaatcggca ccgtattttt ctgctaattc tctacgctgc gctatgggat cgatcccaac
    17641 gatggtagta gcacctgctt ttttagctaa ttgaatcgca atttgcccga tagcgcctaa
    17701 accgaagatt gctacagcat cacctgctcg aacatgtgct tcccggatac cacctaaagc
    17761 gaattgagca ggatcgtaac atactgcatt tttccattga tccaaccgat caagtttacg
    17821 tagtttataa ttatcaacac cgtttgtaat cagcgtttca cgaatgggtc cataagtgca
    17881 gactaactct cctttttgat actctgtcac caaactgccc gtttcttcga tcgtaccgac
    17941 aatcatattt cctaattgaa attcgccaaa taccacaccg cgttggcttc cttcttctct
    18001 tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg ataaatggac ttaatcctct
    18061 aaagtcgaca atttctgttc ctggttttgg agtagcgaaa attactcgga tacgtacttc
    18121 gttcttttcg atctcacggt cttcgtaatc tttgaatccg gctattcttg gttctaatgc
    18181 aactaatttt ttcatctttt ctttcctctt tcttttcttt ttatcctttt acgccgcctt
    18241 cgactaaatc acctttaata aatcgttcag ataacgcata cataatgatg actggtaaag
    18301 cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta gtctggtgta ctgaataatg
    18361 tgttcaaacc gattggtaaa gtaaattttt cggaggtcga caggaaaatc gaagcaaata
    18421 aataatcatt ccacgaaacc ataaagcaat aaacgaagac agaaatgatt ccagaaatag
    18481 ccaatggtat aatgatccga ataatgatct ggatccggct aagaccatcg atcattgctg
    18541 cttcttcaat atcagtcgga atcgtgtcaa aataactttt tagcatgaat atcgcagtag
    18601 gaagtgtttg aacgaccatc gtgataatca gtgctgtatt ggtatcatac aaacctagac
    18661 cggaaatgat tttaaataat ggaacaacga tcaaaatacc ggaaaacata taaacggtgt
    18721 aaaagctagc attgattttc gagcgagctt tgaagcgtag ttttgacaaa gcgtatgccc
    18781 cgctgatccc aatcactact gcagttacgg aggctatcaa ggagatcact aaactatttt
    18841 tgaaatacat gatgaatgga aaaatctcag cattgaaaat atcctgataa tgttcaaaag
    18901 tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc ttcttgtgaa tccttgaatg
    18961 atgtcatcag catgatcaaa aatggaaaca aattgacagc taatagcaac aaaatcgtcg
    19021 cgtaaaataa gattttcctg ctctttttat ttttcatttc aagtccacct tctttcgtga
    19081 gagcaagacg accgctgcaa taatgatgaa caatagaatc gatatagctg ctgcttttcc
    19141 aagatcattg aacgcaaagg ctgtctgata caaataaacg cctaagatat tgactttatt
    19201 cgtcagtaga tagacatctg taaacatata gaacatccag atagatcgta aaacaatcac
    19261 agttgctagt actggtttga ttgccggcag tgtcacgatc ttgaacttct gccaagcatt
    19321 tgcaccgtcc atttcagctg cttcataaag cgttttgtcg actgtttgca aaatagccaa
    19381 aaatgaaata aacgcatacg gatagtatct ccagatagca aacaaaacga ccataaagaa
    19441 gctagcaact ggctggtcga accacagcgg tgcttcgttg aaaagctgca acctatctac
    19501 taacagatca ttgacgattc cataactatt attgaacata tatttccaag caaaaatcaa
    19561 agaaatagat ggcgtaacat aacttaataa aatcagtgaa cgcaccgttt tcctaaatct
    19621 gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa gctgtactgc ctgctacaac
    19681 taagaatgtg taagcaactg ttaaacctaa agaacgataa aattcactat cacttaaaat
    19741 ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag ttaggattca gcggcatatc
    19801 gaaaaaactg atctgaatat tggaaagcat tggaatcagc actaaaacag ctaataaaag
    19861 catgcttggt gctaaaagtc ccatggctaa ttttaagtca gaatgtttcc ttggacgatt
    19921 cattgaaacc tccccctgat aatctgccaa tagctatcta cttttttcga acaccattgg
    19981 cagaacttct tgttatttcg tatttttttg tgcgtcagct aaagcttgat cgacatcttc
    20041 gtttccgaca gttacttggt tcactgcttt tccgatcaca cctgagcttg tgatatctcc
    20101 cattttcgtg aaattctttt ccccgactag tccaaagact tgtacttcat caaaagcact
    20161 agcaatctca gatggcagct cgccaaaaga cttgatgact tcattttctt tgtaaccatc
    20221 cagttcgact acttgtttat tcactggttg agcaccacca ggagacatca agacccactt
    20281 ttccatgttt ttcggttgag ataagaaagc tgtgaatgct tcagcagcct ctttttgttt
    20341 ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg ccatagacag cttgatcttt
    20401 ttgtgttggg atagcaaaac cgatattttc tgagttcccc gcttcataaa cagaaggcaa
    20461 aatgtaagtt gaatagattg ccattggtgc tgttccattc atgaaagcat ctttgatttc
    20521 tgtcacatca ttagagcctg gcatcgtgta ttgggataag ttttgataaa acgacaatgc
    20581 ttctctcatc ttttccgtat cgatcgttac ctcgccttca tcatccaaaa catttgctcc
    20641 atttgacaaa gcaaattgag agaacgcttg ctcactcatt gttccttcag cagtaggcat
    20701 agcaattcca tattgtttat tctctttatc tgtgaaatgt tgagcaattt ttaaaatatc
    20761 atcccaattt tctggttctg aaaaaccagc ttctgataat ttttctttgt cataccaaat
    20821 accttgaacc cagccgctaa ttggtgcagc aatatagctt tttccatctt ctgaacgaac
    20881 aaggtttttg gctccgtcat aataattatc ttctccagct tcctcgataa cgttttggac
    20941 tgccgtctga tcaatcagtt catctttatc catcacttta gcaaaatcct gactgacttc
    21001 cattacttct ggtaatttac cggaacgagc taacgtaacg atttttgtat tgaacgctga
    21061 ttcttctacc ggcacttgct tgattttgat gtctggattt tctttttcaa aatcagcaac
    21121 cagttcattg atcacactta atcgttcctg ttcgacagaa gagtgcatga attgaatcgt
    21181 tgtctgttgt gattcgttag agctttgact ttcaccacaa gcagtaagaa gaaatccagt
    21241 agctagcaga caggtagtaa caataatttt tttcattttt ttccacctct tattttatta
    21301 ttttcagcca catatattca tacggctgga atatcaactt tcgttcttga ctgcaatgga
    21361 taatttgtcc agaaaacagg tctaggtaat ctccttttgg taaaagataa cctgctgatt
    21421 gattggaaaa attatggata actaagactt tctcttcctt cgcttcgcga ataaaactga
    21481 taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt cggatggaag gctttttctt
    21541 tttttcgaat agatagcatc tgggtcaatt caccatatac tgctgttctt aatggatctg
    21601 tctgcaactc tttctcaata tctgctaaca aaaatttttg ccgattgatt tttcgattct
    21661 ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc cagcacactt tgaatataaa
    21721 ttgccggcac gcctggtaac gttaccaaaa atgaatgagc agctaaaaag cgtttgcttc
    21781 tccattccgt cgtatctgtt ggagaactta acgcatctag atacgtagcg tttatctcat
    21841 atggactggt cgttccatct gggtttttct tgtagtttac tcgggcaccc tctttcttca
    21901 gctgttccac taattcaagg atcgtttttt ccggcagaat acctcgtacc ggattcaatc
    21961 cgataccgtc atgggaggct aaaaaattaa aaaaagttgt ttttttcgat gcgaaatcga
    22021 tggtttttaa ccattctttc aacttacttg tattacccga acgaatcgct tgaagggtca
    22081 aaggtggtag cgaaaactga tagaccatct gtgcttcatt ctcgccattc ccaaaataac
    22141 tgatattatc tttgtgtggc acatttgtct cagtgatcaa taccgttcct ggagctgctt
    22201 catcagcgat tgcacggaat aattggatga ttttatgcgt ttctggcagg tgtatacaag
    22261 aagttcctag ttctttccat aaaaatccca cagcgtccaa gcgaataaat gaagcgcctt
    22321 tttctaagta aaacaaaaga atatcgatca ttttcaataa aacttctgga tttttaaaat
    22381 tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg tttttctttc ccgtttttgt
    22441 caatgaacgt tgtaagtaac gggctggtcc ttggtctagt cacgctgctt gtatctgtat
    22501 ccagtggcaa atcaataaaa aaatctttgt acgtatcatt caagtttaaa tattcctgaa
    22561 accaagtact ttcggcagac atatgattgc agacaaagtc gaacattaac cgtgtttcgc
    22621 ttctcatttc ttctatatct tcccatgtgc ccaaacggtc gttgattgcc tgataattta
    22681 caacggagaa accatcatca gatgtatatg ggaaaaatgg caagaaatgt acaactctaa
    22741 atgtatctgc caaatacttt tgataaaatt tcttaaatgt acgcaaagtt ggttgctgct
    22801 cttcaaaaaa ttgatcccca taagtaatca agaaaatatc ttcttcgtcc caatgcagtt
    22861 tgcctgcctg gcctttcttc tgctgataaa cactgacttt ttgttcaatc atagaggcgg
    22921 ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa aagttttgta tacatcggat
    22981 tcccctttct tttttgggag cgttcccaac gatttgatca tattatggaa aacgcttact
    23041 gtcaatagca tttttttaaa taattttgat atacacaaaa atacaaataa gaaagcttat
    23101 gtatcaataa ttaatagctt cttaaaaaaa tatggtctct catgatcatg caagctattc
    23161 ctaaattttc aaaatcatag acaaaaaaga atgtctattg tactatggaa ttattctaaa
    23221 acgctcccaa aaaggaggtg catataaaat tgaaacctac tatttatgac attgcgaaaa
    23281 aagctaatgt ctcaaaatca actgtctcca gagtattaaa taaccagcca aatatctctg
    23341 ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt gaattatgaa ccgagcaaaa
    23401 ttgcaagagg actgtcagga aatggttttg acgcgattct agcattatct catcgtactt
    23461 ctcatacgac ctatggcaat cctttttttt cagatgttat tcaatccatt tctgccgttt
    23521 ctgaacaaaa taacttcgac ttgattctac aaacggcaaa aacaccagaa gaagagctag
    23581 aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg aattattatt ttaagctcta
    23641 cgatggatga aacacttctt tttgaattgg atcaatacaa tattcctatt gtggtaatcg
    23701 gaaaagtcgg aaacgactac acgaacatct actcagtgga cacggataat ttccaggata
    23761 gttacgattt ggtggaaaca ctgatcaaga atggacaccg tcatatcggc tgtctccatg
    23821 ctccgacaga agtttatgta tccaatgatc gtctcaatgg ttaccgtcaa tgcttatttg
    23881 atcaccacct agatatccgt aatgaatgga ttatagacgg cggctttcgc ttagaagata
    23941 gcatggcagc aatgaatgat ttaatgaagc tacgagaaag accaactgcg attttttcta
    24001 ccgatgcatt aaaatcattg agtatttaca aagtggcaga acaatctgga aagcagatac
    24061 ctgatgatct ttcgatcgca ggattcagtg atcccttatt gtcctcccta ttttcgccac
    24121 agttgactcg gatagacatt ccgacaaatc agttaggaat caaagccaca gaacttttat
    24181 tccaactaat ccaccaccaa taccctgaag aaaaaagaat cattctttca accgagatta
    24241 ctctcacaga ttcgatccaa caacaagaaa tgtagaacag aattataaaa gaacagaaac
    24301 actgaggata agtcagttac tctgttcttt tttcaatcac tcatccctcg ttaccccctc
    24361 ccggtatttt agcgaatttt tcttattttc catctgtttt agatagctgt ggataactat
    24421 aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa actattttta accacgttgt
    24481 gtataagttt ttatagttcg tttttttaaa cacatttacc cttatttcaa tccatgttat
    24541 ttggggatac ttatctctaa aacataaata gaataacaga catgtctact ggttttgttt
    24601 tattcgagga attattcaat aatagctagc aaaatattaa aaataaccgc tctatcttcc
    24661 catcaattta ttttgcattc aaaaaatgat ctatctttac ttttgttgat tgacttctct
    24721 tggaagatga tcgttccgat accttaattg gatacgaacc gatgttgtta agatcctttc
    24781 ccacttttct tctagttaga caaattattt tagacaaacc aaaaaagagg ttgtgacaat
    24841 aatttgtcac aacctctttt ttaaaatatc tattttgttt gattgttcat tgcgtcttct
    24901 gcagccattt gcgcttcaat atctgaaata ctgtagacat tttcgcttgc tacaccgact
    24961 tcttttggtt ccatgtcacg ataacgttgc ataccagtac cagctgggat gatcttacct
    25021 ataataacat tttctttcaa accaagaaga ggatctttct taccgcggat cgcagcatcc
    25081 gtcaagacac gagttgtttc ttggaatgat gcagcagata agaaactatt tgtttctaat
    25141 gatgcttttg tgatacctag taagacaggt cgagaagttg caggaacgcc tcctgagact
    25201 agcgtattgt agttttgatc tttaaagtct gcgatgtcta ataatgtacc tggtaagata
    25261 tctgtatcac ctggatccat tacacgaact ttacgcagca tttgacgaac cattacttcg
    25321 atatgcttgt cgccgatttc taccccttgc atacggtaaa cacgttgtac ttcacgaagc
    25381 aagtagtttt caacagataa tacatcgcga acttgcaaca attgttttgg atcgattgat
    25441 ccttctgtca atggtgcacc acggtggatc atatcgcctt ctgctacttt catacgtgct
    25501 gtataaggaa cagtatacgt acgtgtatct gtttttcctt tgattgttac ttctttggta
    25561 cgtgttgcag gatcttctga gatatcgatg acatcccctg ttacttctgt gatcactgct
    25621 tgccctttcg gattacgtgc ttcaaagatt tcttggacac gaggaagacc ttgagtgata
    25681 tcgtctccgg caaccccacc tgtatggaat gtacgcatcg tcaactgagt accaggttca
    25741 ccgattgatt gagcggcgat cgtaccaact gcttctccga cttcaacgtc agagccagtt
    25801 gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt ttgtattaca tgtgaagaca
    25861 gaacggattg tcactgtttc gacacctgca tcaacgattt gttttgcgat gtcttcggaa
    25921 atcaattggt ttcctgcaat gatcaattca tttgtttcag gatgacgtac ttctttacgg
    25981 gtgtaacgtc ccactaaacg atcttctaat gattcgatga tttcatttcc ctcgcggatg
    26041 gcttggattt caagacctcg gtcagtaccg caatcttctt cacggatgat tacatcttgg
    26101 gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg cagtcttcaa ggctgtatcg
    26161 gtcatccctt tacgagcccc gtgagttgag ataaacattt ccaagacgga tagtccttca
    26221 cggaagttcg aaatgattgg caattccatg attcgaccat taggagcggc catcaaacca
    26281 cgcattccgg caagctgtgt aaagttggaa atgttaccac gagctccaga atcagacatc
    26341 atgaagattg gatttttcgc ttccaaacct tccatcagtt tttgttggat gctgtctttt
    26401 gcagcattcc aaacagcaat gacacgttca taacgttcgt catctgtgat cagaccacga
    26461 cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat tttcaatgat ttcttgtttt
    26521 tcattcagta cactgatgtc agcgatccct actgtgatac cggcatgtgt cgaatgtttg
    26581 taccctaagt ctttcatacg gtcaagcatc ttagaagttt ctgttacttt gaatcgtttg
    26641 aagacttctg cgatgatgtt tcctaagttt ttcttcttga acggtaatac taattcttgc
    26701 tctttgatat gtgcaggaat atcagtacca gcttcaacaa agtatttgtc cggtgtttgg
    26761 acggtcaagt tgaagtctgt tggttcattc agataaggga attcaggagg catgatctcg
    26821 ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt ctttttgcca ttcagtaaat
    26881 ggtttttcac ctaatgagtt agggttcaca ccgatacgtg aatgtaaatg aacatatccg
    26941 ttgcgccatg cgataacggc ttcgttttga tctctgaaga ccattccttc gccttcgcgt
    27001 ccttcttctt ccattgtcaa gtagtagtta cctagaacca tatcttgaga aggagtaaca
    27061 actgatttac catctttagg gttcaagatg ttttgagcag ctaacatcaa catacgtgct
    27121 tctgcttgtg cttcttcatt caatggaacg tggacagcca tttggtctcc atcgaaatcg
    27181 gcattataag cttcacatac aagtggatgc aaacggattg cgcgtccttg tactaaaact
    27241 ggttcgaatg cttggatacc aagtcggtga agcgtaggtg cccggttcag caatactggg
    27301 tgttccttga ttacgtcttc taaaacatcc catacttcgt cttcttgacg ttcgatttta
    27361 cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa caagctcacg catcacgaat
    27421 ggtttgaata attcaatggc catttctttt ggaagtccac attggtacat cttcaagaaa
    27481 ggtccaacaa cgataacgga acgacctgag tagtcgacac gtttacctag caagttttga
    27541 cggaaacgac cttgtttccc tttcaacata tgagaaagag atttcaatgg gcggttacct
    27601 ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca atgcatctac tgcttcttgc
    27661 agcatacgtt tttcattttg aacgatgatt ccaggtgcat tcaagtctaa caaacgtttc
    27721 aaacggttat tacggttgat tacacgacgg taaagatcat tcaagtcact tgttgcaaaa
    27781 cgtccacctt ctaattgaac cattgggcgt aaatctggtg gaataacagg gataacgtcc
    27841 ataaccatcc agcttggttg atttcctgaa gcgcggaatg cttctaaaat atccaaacgg
    27901 cggattgcac gtgtacgttt ttgtccttgt gcagatttca attcttcttt caattcggct
    27961 gcttctgcat ctagatcaac attatcaagt aattgtttaa tcgcttctgc acccatagca
    28021 gcttggaatt cttgtccata ttgttcacgt ttttcgcgat attcgcgttc tgtcaacaat
    28081 tgttttttct ctaaattcgt atttcctggt tcgatgacta catatgaagc aaaataaatg
    28141 atttcttcca aagcgcgtgg gctcatgtct aatacaagac ccatacgtga aggaatccct
    28201 ttaaagtacc agatatgtga aacaggagct gctaattcaa tatgtcccat gcgttcacgg
    28261 cgaactttcg aacgagtaac ttccactccg catcgatcac aaacgattcc tttataacgg
    28321 atacgtttgt attttccgca ggcacattcc cagtcttttg taggaccaaa aatccgctca
    28381 cagaacaacc cttcgcgttc aggttttaag gtacgatagt taatcgtctc aggttttttt
    28441 acttcaccgt aagaccagct tctgattttt tcgggagaag ccaaacctat ttgcatactt
    28501 tcgaatttat ttacatcgat caaaaggggt tccctccatt tcatttaatg gcaaaccgca
    28561 agacggctga tcttcgaccg ccttgcagct catagaccgg attagtctaa attatcttct
    28621 gcggtttcaa agttttggat gacatcgtct ttttcatctt ccacttgttc agcagctttc
    28681 ttttctaatt ctttggcagt ttgctgttcg gcaaattttg tcaaagcatc aacagtgatc
    28741 aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct ctgtttcttc gatatccaag
    28801 acacgcatgt ccagacctag tgattgcaat tctttcacca atacgcggaa tgattccggt
    28861 acacctggtt ttggaattgg ttcacccttg acgattgctt cgtatgtttt cacacgtccg
    28921 actacgtcat ctgatttgta tgtcaagatt tcttgtaaag tataagccgc accgtaagct
    28981 tccagtgccc atacttccat ttcaccaaaa cgttgtccac caaattgagc tttacctccc
    29041 aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac gtgcatgcaa tttgtcatcg
    29101 accatgtgag caagtttgat catgtacatc acaccgacag acacacgtcc atcaaatggt
    29161 tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc tggccatacc ggcttcacga
    29221 actgtttccc atacgtcttc atcacttgca ccatcaaata ctggcgttgc tacgtggata
    29281 cctaactgac gtgcagccat acccaagtgt aattccaata cttgcccgat gttcatacgt
    29341 gaaggcaccc ctaaaggatt cagcatgata tcgattggtg ttccatcagg taggaatggc
    29401 atatcttctt ccggcataat acgggataca acccctttat ttccgtgacg accggccatt
    29461 ttatcccctt catggatttt acgtttttga acgatataaa cacgaactaa catgtttacg
    29521 cctggtgaca attcatctcc agcttcacga gtaaagattt tcacatcgtg aacgataccg
    29581 ccgccgccat gtggtacacg taatgatgta tcgcggactt cacgagcttt ttcgccaaag
    29641 atcgcatgca atagacgttc ttctgcagat aattctgtta ccccttttgg cgtgactttc
    29701 cctacaagca aatcgccatc ttggacttct gcaccaatac ggatgatacc catttcgtct
    29761 aaatctttta atgcgtcttc cccgacatta gggatttcgc gtgtgatttc ttcaggtcct
    29821 aattttgtat cgcgggcttc tgattcatat tcttcaatat ggatagaagt gtatacgtca
    29881 tcttttacca aacgacggct catgatgatc gcatcttcat agttgtaccc ttcccaagtc
    29941 atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc cttgttccat agatggtccg
    30001 tctgccagag tatccccttt ttcgactttc tcacccaaat gaacgattgg acgttggttg
    30061 tagcttgttc cggagtttga acgacggaat tttgttacat cgtatttatc taatgcaccg
    30121 ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt caacgacacc gtcatgttta
    30181 cataacaaag cagctcctga gtcatgcgct gatttgtatt ccataccagt accaacccat
    30241 ggtgcttgag gattgatcaa cggcactgct tgacgctgca tgttggcacc catcaaggca
    30301 cggttggagt catcgttttc caagaaagga atacatgctg tcgcaacggc aactacttgt
    30361 ttaggtgata cgtccatgta gtctacttta tcaatagata cttctaagtt ttcactttga
    30421 gcacgagcca taacaacgtc ttgagcaaat gttccatctt cattcaatgg gctgtttgct
    30481 tgggctacga tgtaatggtc ttcgatatca gctgtcaagt agtcgatttg gtcagtaaca
    30541 cgacctgttt cacggtctac acgacgataa ggtgtttcga taaatccgaa tttatttact
    30601 ttcgcataac tggacaaact attgatcaac ccgatgtttg ggccttcagg agtttcgatc
    30661 ggacacatac ggccatagtg ggagtagtga acgtcacgaa cttcataacc ggcgcggtca
    30721 cgagtcaatc cgccaggtcc taaggctgaa agacgacgtt tgtgtgtcaa ttcgccaaga
    30781 gggtttgttt gatccatgaa ctgtgacaac tgagaagaac caaagaattc tttgatactt
    30841 gccacaactg gacggatatt gatcaattgt tgaggtgtca atgtttcagt atcttggatc
    30901 gacattcttt cacgaaccac acgttccata cgtgctagcc cgatacggaa ttggttttgt
    30961 aaaagttcac cgactgaacg gatacgacga tttcctaagt ggtcgatatc atcgacattg
    31021 ccgatacctt ccatcaagtt caagaaatag ctcattgaag caatgatgtc ggctggacga
    31081 accgttttta cagctgcttc tggatagccg tttccgatca cgtttacttc acgttcaggg
    31141 tctcttggtg aaaatacttt gatgacttgg acagtcattg gatctgtgac cacaccgtct
    31201 tctgatggat agtaagtcac tgaatttagg ccattatcaa tgaaaggagc tagtgtttcc
    31261 atgacttggt gagtcagaac tgttcctttt tcaacgatga tctcgcctgt ttctggatca
    31321 actaatgttt cagctaatgt cagattcaat agacgtgttt tcaaatctaa ttttttgttt
    31381 actttgtaac gaccaacgtt tgctaagtcg taacgttttg gatcgaagaa acgagcattc
    31441 agcaagttac gtgagctgtc tgctgttttt ggttcgcctg gacgcaaacg ttcgtaaacg
    31501 tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt tatgcaagtc tttttcgact
    31561 gtattgcgta atgtttcgct gtcgccgaag atttcaaaga tcgtatcatc ggaaccaaat
    31621 cctaacgcac gaactaatac ggttaaagga atcttacgtg tacggtcgat acgtacataa
    31681 gagatgtctt ttgcatcagt ttccatttct agccaagctc cacggttagg gataacagta
    31741 gaaccgaatc cttcttttcc gtttttgtcg acttttccat ggaaatagac acctggtgaa
    31801 cggactaact gagaaacgat tactcgctct gccccgttga tgatgaatgt tccttgttct
    31861 gtcattaatg ggaaatcacc gaagaatact tcttgtgctt tgatttcgcc agtctcgcgg
    31921 tttgtcaaac gcaatgttac atgtaatggt gcagagtagt ttgcatcatg cgcacgagct
    31981 tctgctactg tatactttgg ttctttcaat tcataatcta caaattccaa agatagattg
    32041 ccattgaagt catcgattgg caaaatgtct tcaaacattt ctctaagtcc ctcatctaag
    32101 aaccattggt aagagtctgt ttgaatttcg atcaaatttg gtaattccaa cacttcactg
    32161 attcttgcga aacttctacg ttcgcgatgt tttccgtatt ttactacgtg tccagccaag
    32221 ctcttcaccc ctctaaaatg tcattcaaaa agactacccg atcaattgtt acaataatat
    32281 ttcaaatatt atttttaggt aacaacagac attttttgcg gcttttaggc aaaaaaaaac
    32341 caaaaataga acattctctt gaaaccttac ttcgtgagct tctacttttg tttccttata
    32401 ttaaagccga aacggacaat atttcgtttc tgccttttct tcatcagata gtttttgcaa
    32461 cttcatatac tacacttatt ttctataaaa gtcaacactt cctggcgttt cttctgcttt
    32521 ttcgcaacat tttcttaatg ttttcaattt tatgttttca attatctgac aattcttaat
    32581 tgacagacct ttttatctcg gttatacttt agaaaattca ttttgtggag gaacttctat
    32641 gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat aacttttact ttagctactt
    32701 tagataggtt tgtattcatc atgatggata caaacgttaa acgacagttt gtatctatga
    32761 tggctaaaga actttgtatt gcagcaaaaa gccacgtaga caaaatgcta agtggctgag
    32821 cagtgaaaat acaaagtaaa agtatcaagc attctacttt ttcaatttat tttccgactt
    32881 ttcaatccat ttggacattt tgtctgaatg gattttttgt ttcccaaaat taaggaggaa
    32941 aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg ccctgctact agctttagga
    33001 ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg gacaaaaaac aattggcgta
    33061 ttgcaacttg tacaacataa ctcgttagat gctgcctata aaggattcaa agaaggatta
    33121 gcagaagaag ggtataaaga aggcgataat ctaacaatcg attatcaaaa tgcccaaaac
    33181 aatcaagaca acctgaaaag tatgagtgag aaactcgtca aagaagatcc tgatttgcta
    33241 cttggaatcg ccactccagc agcacaatct ttattaaatg aaacaaccac tatcccgatg
    33301 actgtcacag cggtcacaga cttggaacaa gcccatttag tcacatccaa tgaaaaacct
    33361 ggcggcaatg tgactggcac gacagatatg gtgccaatcg aaaaacaaat cggcttactg
    33421 ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt ataacaacgg tgaagcaaac
    33481 tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa aagcgggcgt caaagtcaaa
    33541 gtactgacag caaattccac aaatgatgtc caacaagtga cgaaaagtct agctaaagat
    33601 gtagatggaa tctatattcc tactgataac acgttcgcaa gtgcggcaag tgttgtcggt
    33661 gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg cagttgatca agtcaaagac
    33721 ggcggtctag caacagtagg aatcgactac gaaagcttgg gcaaacaaac agggatcatg
    33781 gcagcaaaaa tcttagacgg caagaatcca agtgaacttc ctgtcgaaac agcgaatgat
    33841 ttggaactgt atgtcaataa agacatggcc aaagcattag gcatcaatcc agacagcatc
    33901 aaagaagctg aataaaacac aggaggattc tcatgacaaa tttgctctct cttttcttat
    33961 catctgcatc gcaaggggtc ttatgggccc tacttgcgat tggtgtatac gtcactttcc
    34021 gtatcttgga cattgccgac ttgactgctg aaggaagctt tcctttaggt gcagggatcg
    34081 cggcagtaag tattacaaat ggctatcatc cattgacagc ttgtcttcta ggctttttag
    34141 ggggtgcggc agcagggctt gtttccggac tgcttcacac aaaattgaaa attcccgccc
    34201 ttcttgcagg gatcatcacc atgactggtt tatattctgt cacatcccgt gtgatgggtg
    34261 cgccgaacat ctctttattg ggtcagccaa cgatctttca ttgggtcgag tcattcggca
    34321 taacaaaaga caacgctgta ctgatcgtcg gtctgctttt tgctttgatc gttgtatgtt
    34381 tacttgtttt gttcttacgt acagaaacag gacttgctat tcgtgcaact ggcgataatc
    34441 tggcaatgag cgaagcaaac gggatcaaca cagataatat gaagatcatc ggttatatga
    34501 tttctaatgg atgcattgct ctttctggct ccttgcttgc gcaaaacaac ggctttgctg
    34561 atttaaattc tggtattgga acgatcgtaa ttggacttgc ttctattatc atcgcagaag
    34621 ttttgttacg taaccagcca ctatttctcc gtctcttgac tgtcgtttta ggtgcggtca
    34681 tttatcgttt tattttagcg ttagtcttcg aactgaatgt tgaaccaagt gattcgaaac
    34741 ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc gcagatccag caaaaattcc
    34801 atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat gacgtcttaa cattggaaaa
    34861 tctccatcat acctttgaaa aaggaacgat caacgaaaat catgtcctga aaggcattaa
    34921 tctttcctta gatcccggcg acttcgtgac gatcatcggt ggaaatgggg ctggtaaatc
    34981 tactttgctg aacagcatcg ccggtacatt ttctgtcgat cagggtcaaa ttttattgaa
    35041 tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg aaatccatca gtcgcgtatt
    35101 ccaagatcct aaactaggga cagccgttcg tttaacggta gaagaaaatc ttgctcttgc
    35161 tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc aaaccacaag accgaacatt
    35221 tttcaaagac cagctggttc ggctaaattt ggggttggaa aatcgtttat cctcagaaat
    35281 cggtttgctg tctggaggac aacgtcaagc tattacgtta ttgatggcca cattacagcg
    35341 tccagaactg atcttattag atgaacacac tgcagcgctt gaccctgcta cttccaaaac
    35401 ggtgatggaa ttgacagaaa agctgatcac tgagcagaat ctcactgctt ttatggtcac
    35461 tcatgacatg gaagacgcga tccgctatgg gagtcgactg atcatgctgc atcaaggaag
    35521 gatcgtcgta gatatccagc aagaggaaaa acagcagctc acagtgcccc aattattagc
    35581 gttgttcaaa caaaacagcg gcacggcatt aaaagacgat caagtacttt tatcttcata
    35641 atcagaaaga ggatgggaca aaa
//
LOCUS       DACEKG010000024        35598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEKG010000024 DACEKG010000000
VERSION     DACEKG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.024"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW664_001073"
     CDS             5..403
                     /locus_tag="IW664_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0848451.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW664_001074"
     CDS             387..1082
                     /locus_tag="IW664_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0848452.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="IW664_001075"
     CDS             complement(1666..2553)
                     /locus_tag="IW664_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0848453.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="IW664_001076"
     CDS             2738..3577
                     /locus_tag="IW664_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0848454.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="IW664_001077"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="IW664_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0848455.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="IW664_001078"
     CDS             5045..5401
                     /locus_tag="IW664_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848456.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="IW664_001079"
     CDS             5404..6561
                     /locus_tag="IW664_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848457.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="IW664_001080"
     CDS             complement(6587..7027)
                     /locus_tag="IW664_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0848458.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="IW664_001081"
     CDS             7187..7693
                     /locus_tag="IW664_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0848459.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="IW664_001082"
     CDS             7861..9774
                     /locus_tag="IW664_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0848460.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="IW664_001083"
     CDS             9779..10780
                     /locus_tag="IW664_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0848461.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="IW664_001084"
     CDS             complement(10982..11188)
                     /locus_tag="IW664_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0848462.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="IW664_001085"
     CDS             complement(11834..12682)
                     /locus_tag="IW664_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0848463.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12846..13748
                     /gene="gnd"
                     /locus_tag="IW664_001086"
     CDS             12846..13748
                     /gene="gnd"
                     /locus_tag="IW664_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0848464.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13750..14751
                     /locus_tag="IW664_001087"
     CDS             13750..14751
                     /locus_tag="IW664_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0848465.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14748..15032
                     /locus_tag="IW664_001088"
     CDS             14748..15032
                     /locus_tag="IW664_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0848466.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15065..15862
                     /locus_tag="IW664_001089"
     CDS             15065..15862
                     /locus_tag="IW664_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0848467.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15876..16709
                     /locus_tag="IW664_001090"
     CDS             15876..16709
                     /locus_tag="IW664_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0848468.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16727..17584
                     /locus_tag="IW664_001091"
     CDS             16727..17584
                     /locus_tag="IW664_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0848469.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17606..18115
                     /locus_tag="IW664_001092"
     CDS             17606..18115
                     /locus_tag="IW664_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0848470.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18152..18553
                     /locus_tag="IW664_001093"
     CDS             18152..18553
                     /locus_tag="IW664_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848471.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18665..19018
                     /locus_tag="IW664_001094"
     CDS             18665..19018
                     /locus_tag="IW664_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848472.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19052..19495
                     /locus_tag="IW664_001095"
     CDS             19052..19495
                     /locus_tag="IW664_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0848473.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19512..20597
                     /gene="uxuA"
                     /locus_tag="IW664_001096"
     CDS             19512..20597
                     /gene="uxuA"
                     /locus_tag="IW664_001096"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0848474.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20590..21303
                     /locus_tag="IW664_001097"
     CDS             20590..21303
                     /locus_tag="IW664_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0848475.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            21632..22933
                     /locus_tag="IW664_001098"
     CDS             21632..22933
                     /locus_tag="IW664_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0848476.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            23556..23756
                     /locus_tag="IW664_001099"
     CDS             23556..23756
                     /locus_tag="IW664_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0848477.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            24324..24887
                     /locus_tag="IW664_001100"
     CDS             24324..24887
                     /locus_tag="IW664_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848478.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(24948..25292)
                     /locus_tag="IW664_001101"
     CDS             complement(24948..25292)
                     /locus_tag="IW664_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848479.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(25389..25634)
                     /locus_tag="IW664_001102"
     CDS             complement(25389..25634)
                     /locus_tag="IW664_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0848480.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(25704..25958)
                     /locus_tag="IW664_001103"
     CDS             complement(25704..25958)
                     /locus_tag="IW664_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848481.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(26122..26445)
                     /locus_tag="IW664_001104"
     CDS             complement(26122..26445)
                     /locus_tag="IW664_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848482.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            26632..26705
                     /locus_tag="IW664_001105"
     tRNA            26632..26705
                     /locus_tag="IW664_001105"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26666..26668,aa:Ile,seq:cat)
     gene            complement(27090..27587)
                     /locus_tag="IW664_001106"
     CDS             complement(27090..27587)
                     /locus_tag="IW664_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0848483.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(27627..28436)
                     /locus_tag="IW664_001107"
     CDS             complement(27627..28436)
                     /locus_tag="IW664_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0848484.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            28770..29627
                     /locus_tag="IW664_001108"
     CDS             28770..29627
                     /locus_tag="IW664_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0848485.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            29620..30522
                     /locus_tag="IW664_001109"
     CDS             29620..30522
                     /locus_tag="IW664_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0848486.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            30555..30905
                     /locus_tag="IW664_001110"
     CDS             30555..30905
                     /locus_tag="IW664_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0848487.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            30939..31877
                     /locus_tag="IW664_001111"
     CDS             30939..31877
                     /locus_tag="IW664_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0848488.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            31955..32848
                     /locus_tag="IW664_001112"
     CDS             31955..32848
                     /locus_tag="IW664_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0848489.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            32886..33449
                     /gene="amaP"
                     /locus_tag="IW664_001113"
     CDS             32886..33449
                     /gene="amaP"
                     /locus_tag="IW664_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0848490.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            33463..33651
                     /locus_tag="IW664_001114"
     CDS             33463..33651
                     /locus_tag="IW664_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0848491.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            33664..34566
                     /locus_tag="IW664_001115"
     CDS             33664..34566
                     /locus_tag="IW664_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0848492.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            34598..34840
                     /locus_tag="IW664_001116"
     CDS             34598..34840
                     /locus_tag="IW664_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0848493.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(35096..35467)
                     /locus_tag="IW664_001117"
     CDS             complement(35096..35467)
                     /locus_tag="IW664_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848494.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT        12350 a         5718 c         7130 g        10400 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
    14161 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
    14221 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
    14281 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
    14341 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
    14401 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
    14461 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
    14521 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
    14581 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
    14641 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
    14701 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
    14761 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
    14821 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
    14881 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
    14941 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
    15001 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
    15061 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
    15121 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
    15181 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
    15241 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
    15301 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
    15361 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
    15421 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
    15481 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
    15541 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
    15601 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
    15661 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
    15721 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
    15781 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
    15841 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
    15901 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
    15961 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
    16021 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
    16081 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
    16141 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
    16201 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
    16261 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
    16321 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
    16381 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
    16441 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
    16501 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
    16561 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
    16621 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
    16681 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
    16741 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
    16801 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
    16861 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
    16921 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
    16981 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
    17041 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
    17101 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
    17161 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
    17221 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
    17281 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
    17341 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
    17401 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
    17461 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
    17521 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
    17581 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
    17641 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
    17701 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
    17761 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
    17821 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
    17881 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
    17941 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
    18001 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
    18061 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
    18121 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
    18181 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
    18241 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
    18301 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
    18361 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
    18421 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
    18481 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
    18541 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
    18601 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
    18661 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
    18721 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
    18781 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
    18841 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
    18901 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
    18961 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
    19021 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
    19081 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
    19141 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
    19201 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
    19261 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
    19321 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
    19381 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
    19441 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
    19501 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
    19561 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
    19621 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
    19681 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
    19741 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
    19801 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
    19861 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
    19921 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
    19981 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
    20041 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
    20101 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
    20161 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
    20221 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
    20281 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
    20341 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
    20401 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
    20461 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
    20521 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
    20581 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
    20641 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
    20701 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
    20761 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
    20821 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
    20881 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
    20941 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
    21001 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
    21061 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
    21121 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
    21181 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
    21241 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
    21301 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
    21361 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
    21421 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
    21481 agaatcgtcc atatgagggt ttccacggct ctataatttc taggactgat agaaaatcat
    21541 tctatcggtc ccttttgagc atgaaaaaac aaaacataca gaagctccgt tagaattgaa
    21601 tcaccacaac acaatcaaag gagcgttctg tatgcctgat cttcatcata tcaaactact
    21661 acttggaatc aaagataaaa acattttcat cacaaatgtg gaatcgaaga gcattaagaa
    21721 tatcaagagt cttgttgttt ctgcgacgtt aagtaaagaa attcgtcgct gtcctttgtg
    21781 taaacagatg aatcatgaag gcatgatcgt taagaacgga aagaaaaaat cgctcattca
    21841 acttaataaa tgtgcgaacc aactgactta tcttgcttta gcgaaacaac gctaccactg
    21901 tcgtggctgc cacacctatt ttacggcgaa tacctacatt gtcgatagaa actgctttat
    21961 cgctaaacaa gtgcgctata aaattttaga agagctaact gaaaaacaag caatgacgac
    22021 aattgctaag cattgcggcg tttcttggtc gaccgtttct cggacactcg cttctctgat
    22081 ccccatgaca aaagtaaaaa gaaattggtt accgcgttgt ttattaatgg atgaatttcg
    22141 ttcactgaaa aatcaagtcg gtccctactc cttttcttgt atggatggcg atactggaaa
    22201 attactggat attcttcctt cacggaagaa aaaggctctc gtttcttatt ttatgcaatt
    22261 tgaacgacgc gcacgattga aggtaaaggt catcgtgacg gacatgaatg tttcttatgc
    22321 ctctctgata aaggaatgtt ttccaaaggc aaaactcgtt gtggatcgtt ttcacattgt
    22381 caaacatctc attcgtaaat ttgaggatat tcgcgtacga atcatgaaaa gctttgatcg
    22441 aaacgatcca acacaagcca aatattatcg acaattaaag gcgttgtctc ggcttctgat
    22501 caaacgacag gacaagcttg tttatgacaa atggaccaaa tggcgcaatt ttggttgggc
    22561 gtatctcacc gaaagtgaag tggtggatcg tctgctttcg atctctgacg aattacgaat
    22621 cgcctatttt tactatcaag aaattcttca agcgtttcac gacaaagagg cggacacttt
    22681 ctttaaactt gtaagaacga tgcccaattc cgttccaaaa gaactgcatt ctataaaaaa
    22741 agcttttata aactatgaat ccggcattcg tttagccctc gaattgcctt attcaaatgc
    22801 caaaatagaa aatctgcata cccacatcaa agcattgaaa cgagtcgctt atggctttcg
    22861 aagctttcgg aaaatgaaga ccagaatttt cttactaaat aatctgatta cctatgaatc
    22921 aaaaaacatc taaagtagaa aaggaattct accttagatg ttcttatctc actctatcag
    22981 tcctatttga caaagagccg tttccactcc ttatgcagat ttttttaatt cttgcactgc
    23041 ttgttcgatc gtaacacctg tcgctacatg attggtattt tctttgtcaa aaacgacaaa
    23101 tagattcaaa tctgtatcca aggttactcg atattgtata tctttgttat agaatgtcat
    23161 agccattctc ctttctttaa atacttgctg aaaggcaacg aacgcgttga taacgaaatt
    23221 ccatttataa gtataccacc aattgaaagg ttttcatagt taagaaattt taaagtcaaa
    23281 agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa
    23341 ctttttcttc gattcgaaga caatagaaga caatgaattt tatatttcat tgcattatcc
    23401 ttgtgttatt agaaaaagca tgatatatta aatgagtaat ctattttgaa acgtaatttg
    23461 agcgatatat ctacatagat aaaactcctt ttgcctatca aatatttatt gcaacgaaaa
    23521 aacgtacatt gatacgtatc aggaggaatt tatttatgaa taacggtaca gtaaaatggt
    23581 tcaacgcaga caaaggtttt ggctttatca ctggtgaaga tggaaatgat gtatttgcac
    23641 atttttcagc tatccaaggt gacggcttca aaactttaga agaaggccaa gcagtgtctt
    23701 acgatgttga agatggtcaa cgtggccctc aagcaacaaa cattgttaaa gcttaataaa
    23761 aattgacttt taaccgtctt ttcgaaagaa aagacggttt ttttgtacga aaaaattcat
    23821 tggtcgttga atgggctgaa tagtagtgta taaaacaatt ttgtggagcg tgaagctttt
    23881 atggtagagg aaaatacgaa taaagtgatg ttgataaaaa gattcctatc ttgaatatga
    23941 atcgagatgt cttgaaaatg aatgagtttg accaatcatt atcctatcga aatttacagt
    24001 ttcgttcgat tggtctgtca attaatgaaa aagtaaaaaa gaatatgttc taggaagagg
    24061 gcggcacatc taaattttta agtaacggac agttcaaata tcaagcattg aaagaggcta
    24121 tcctactgtt tgaaggaata cgaatatata aaaacgacaa agaggaaaaa gtagttcatt
    24181 aaatcccttc aagtgatgaa agaatgagat ctaataaaac ctgatctttc atctgttttt
    24241 ttaataacgt aataattttt ttattttttg tgctttcgcc gttattgtag agtgatttag
    24301 cattcttatt tttaaaaata actgtgttat atattaaaaa aggcataata gttataagct
    24361 tttttaaaga atatttagaa attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta
    24421 ttattttagt ctattttcag ggggggaacc ttatgataaa aaaactattt ttactatcaa
    24481 tattaacgct agcaatggca ggttgttctg actctggctt agcaactgaa ctgatcacaa
    24541 aatcaactga agccacgaca gaggagttaa aatgtggaaa cgatcacagt gactacagag
    24601 gcttaaatcg acgatgcatg ggaatattag gcagaagcaa ttgtaagcat cgatgtagta
    24661 atcgaagaag gtgtagaaga agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc
    24721 tgtgctgtgc atcgtataca gacgtcgaag atgcggtact tttaaaccac gtcaggagat
    24781 gttcatttat gtctagggaa gttggcgatg aaccaaaact agagtacgct tttagttttg
    24841 cacgtgagga tgaagataac cctaaaactt acgacatttt agcttgatct aagatggtta
    24901 aagcttttta atgaagtaag aggagataga aggcttttct agttatatta ggtaagataa
    24961 attccttttt gctccaaatt tgctaaacat tctgctagat aaacttcatc gtgttccgga
    25021 taaatcggca cgttgcgctc tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca
    25081 cgatacatag gatttttcca ctctggtgct gggctgtaac cacgtttttc catttcatcc
    25141 atgacaagct gatgatattg gaataatcta taaggagaat agtgaaaaac ataatccaca
    25201 gtagcatgtt tctttcccca gccatttcct cttaatgcac agcattcccg atgttgtcct
    25261 aaaagctgtt gacgaggaag ctttgaaatc aatttttcat gccataatca catagaaatg
    25321 tctcctttat atatagattg ttaaccttag acgttaaatg aaaaaaccag caccaaggct
    25381 ggaaaaaatc acttcagttt gcttgctgtt tctgagcgac caattaggta atcaacggaa
    25441 atatcgtaaa agtcagcgat atagattaat tcaatgatgc ttggaatttc ttctccttct
    25501 tcgtacttac gatatttgaa taaaggaata cttaacgcgc tagaaacagc tacttgagat
    25561 aaagatcggc cagttcttaa gaaattcaga cgacaacgta aagtatccag taaaatagct
    25621 tcttcgatac tcatttaatc acctttcatt atagtagaat agttataatg actatagaaa
    25681 gctacacatg caatcaatag tactcaatgt tttttattta aacgtatcaa ataaagaatg
    25741 acgatcaata aagacaaaac cagtcctgat aattcttcta taaaaacact atcgggttca
    25801 aagtagttgt aattaaaaac tttccacata aagtagatag ccggaagagt caaaatagct
    25861 aaataaaacc gttctttgtt ccaattcata agataaacaa tacttgcgac cagtgcgacc
    25921 gcaggcaccc aaaacaaaac tctcaccaac gtaatcatgt aacggactcc tcaacgattc
    25981 atataataaa agataacatg ttattgcata catcacaaat gaaatgctag gaaaataaaa
    26041 gaagaataga tcaatgaaaa atgaaaaagc agaacagttt tctgctgata agcaggaact
    26101 gttccgctga atgtgtctta tctatcgaga aaaaatcaat ggattgatat ctaagtaatt
    26161 caatacatat aaaaagagga cgatcgcatt cactaaaaag gaaagaatga tgatcccata
    26221 gcgaatggaa acatatttaa caaatacttt tgtttgaata cgggcatatc tttttccttt
    26281 atagctgagc atcatgacaa gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat
    26341 aaacagaatt cctgaggttt gataatttaa ttggagaaaa taagctgctg ccgcaactaa
    26401 atcgaataaa atcgcaataa atggcaaaat aatcactcct aacattgaga ataaataaaa
    26461 gaataacatt ttttctaaaa taagcaaata aaaattaaag taacaaaata gttatgacac
    26521 aagtaaaaga aataagaata acaatattat tttacttata ttttgaaact tgtgagagtt
    26581 tgtcattcgt gctatagttg aaaagtaaca aaaataaatt ttatttatga tgtaccttta
    26641 gctcagttgg ttagagcaga cggctcataa ccgtccggtc gtaggttcga gtcctacaag
    26701 gtacataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaagtaaaa
    26761 acagcttctt atgtttttac ttggctgttc ttattactgc agatgcaagc ttttgaatac
    26821 cgtttaagga agaggttgtg aaatcagcgt tttgacctga gtattagctt aacaaacgga
    26881 aaaatagttc ctcatatttt gccgtttgtt aagctaatac cgagggtcag ctgattgaac
    26941 accatttaag aaagaggtcg tgagcctgcc gtttcactct gtgtcataaa aaagccaatc
    27001 tactttgata gaaaaagcac gtaggactaa aacagttctg cgtgcttttt ttattgtgtg
    27061 ttaaatatcg taaatgaaga tagatatttt tatgattcga cgatagagac gttgaaatct
    27121 ttcaacagct ctaaagcgga ttctccgtgt tttctactgg agactaacat tggatcaaca
    27181 aaaaagtctg cttcaggaaa agcactctgc aggcctatca gattagagag tacacacaag
    27241 tttgtttcaa tcccgactac ttcgatctct ttttgttctt ctttgctttc aaataaagtt
    27301 tcctggattt cagctaaagt ttcaggtgga atcgtaaagt aatacttttt aatcactcgt
    27361 tcattttctt gtggagaaag aagagggatc aattgcaaat cttctcgttc ttcttctttg
    27421 tccttcagtt caactggtat atcccgtgtg aagagaatat attcattatt ttggcgtgct
    27481 ttagctaatc tttgagcaat acgttctgcc aattgatctg actctggaat ataaaattcg
    27541 ctgtttggat caagaatatg attttgcata tcaataataa ctaacatttt ctttcctcct
    27601 acattcatat cgtcatttta ttctttttaa tagaagtttt ttaaaatata agcaacacca
    27661 gcttcttcat tcgtcagtgt tacttggttt gcaatttctt ttacttgtcg ctgtgcattc
    27721 cccatcgcaa tactcaaccc gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca
    27781 aaagccatga tgtcactcga ttcgattcca aggtaatccg ccagaaaaac aagtgaatga
    27841 gctttagtga tacctgtagg cgtgatttct aagaaatgcg gatcagagaa tactgattgg
    27901 ataccatctg cttgcagatc tctttgtagc ttctgtagtt tttcttcact cttatctgtt
    27961 aatgctaatt taagatagct aagagaagga tcttccaaca tatttaaaat ttgatcaaaa
    28021 gaatggttct gtaaattgaa atgttcataa taagggtcat ctttgactag atttgattca
    28081 tcgaatgtca gctgatcatc ttgggtttct cggctagcaa tgatggatat tttcgcttct
    28141 tgagcataga agaaggcttt tctcagtgta tcgctatcta tttttgaacg tctgagaact
    28201 ttcgccattt ttccatcctt catttcagca atcagtgaac cattggaaga aatcaaatag
    28261 ccaccgattc ctaattcgga aaaaatatct ttagcagaaa tgctgttcct tccggtagcg
    28321 acggttacga ttccgccatt ttcatcaaat ttccgaagtg cttggatatt ttcttctggt
    28381 atctttccat ttcgatcaag caaggtaccg tctaaatcaa tcgctgctag tttcatttta
    28441 tcttcctact ttctttttac atatctattt tcctgttacc ttaagattaa atgagaattt
    28501 aaaaaaatga aactaacttg ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa
    28561 aaaggtggct atatatagta gtttttggtc aatacctttg aataaaaagc catgaaaaac
    28621 gaatagttag cgcttaccga tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa
    28681 acaaagcata tattttgctt taaaaccaaa tgaggaatat tctagatgta gaacaggtta
    28741 gaaaaagatt agaaaaagga gtgattttga tggagaagag atacggcgta gttcctgtgg
    28801 taactgcttc agacgaaaat tatgctcctt acttgagcgt gatgatcgca actgcattag
    28861 aaaattgcaa taaaacaaga cgcataaaat tttatgtgat tgacgatggt ctatcagaat
    28921 atagtaaaga aggattggaa gaaacggtaa ataaatattc aagtaatgca agtatccaat
    28981 tcctgacagt ggaaaaagat atctacgaag acttcctagt cagtgaccat atcacaacta
    29041 ccgcttactt aagaatttct ttaccaaact tattagctaa agaggattat aagaaagtcc
    29101 tttacttaga ttcagatgtg ttagtactag acgatatcgt aaaactatat gatgagccgt
    29161 taaatggaaa aacaatcggc gctattatcg atccaggcca agtgaaagct ttggaaagat
    29221 taggaatcga ttccgatgac ttgtacttca attcaggagt gatggtgatt gacattgatc
    29281 aatggaataa aaaagaaatt acagaaaaaa ctatccatta cctcagtgaa aatggcgatc
    29341 gaatcatcta tcacgaccaa gatgctttga atgctgtact ttatgaagat tgggaacaac
    29401 ttcatccaaa atggaacatg cagacttcgc tgatttttga acgtcatcca gcaccaaatg
    29461 aaaaatatga acgtcagtat aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag
    29521 gacatgataa accttggaat acgttgaaag atcatccgta taccaattta tacttaaaaa
    29581 aattagcaca cagtactcta acgaaagtag gcgagatcaa tgaataaaaa agaaatagca
    29641 gtagtcgcaa gttgtaacac aaaattcgta ccacatttag ctgcattatt tgtctcagta
    29701 ttagataatt gcaacccgtc taaatttgtt cggttctatg tgattgacga tgatatcgat
    29761 tttgaaagta aacagctttt acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg
    29821 gaattcttga aaatcaataa agaattcttt acaaatgtgg taatcagcga tcgtattcca
    29881 gaaacagctt attatcgtat tgcgattccg gaattgttcc gcggcacaga agttgaacgt
    29941 atcttatata tggattgcga catgatcgct ttacaagata tctctaaatt atggcgtctt
    30001 gacttcggtg attcaatcgt agcagctgtt gaagacgctg ggttccatca acgactggaa
    30061 aaaatgaaaa tttcagctaa atcaatgcgt tatttcaact caggtttgat gctgatcaat
    30121 gtgaaaaaat ggttagatga aaatattacg caaaaagtat tagatttcat cgaacataat
    30181 ccagaaaaat tacgattcca tgaccaagat gcattaaatg ctatcttaca tgaccgttgg
    30241 ttaccacttc atccgcgttg gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat
    30301 ccaacagcag caggtgaacg tgaatacgaa gaaactcgca acaatcctta cattatccat
    30361 ttttcaggac atgtgaaacc gtggagcaag gattttgaag ggccaacgaa gaaatattac
    30421 gaaaaatatg cagggatgac ggctttccgt tgtgtagcta aattccctaa atatccaaaa
    30481 tatgcaaaag ttcaaccaaa aacagatgta gttgttaaat aatagaagac aacaactgaa
    30541 gagggggagt aggaatggaa attacaatgt ctaaaaatgc agtagagacg ttaattgaaa
    30601 aggttgggga aaatacaaaa atcgcactcg cgcttatcaa tgattccgat ccatttctaa
    30661 gggacaaagg tgcttttgca aaaggcagct tttttcaaat cattcctttt gtttcggaat
    30721 ttggagaata tgcgactaaa atcgagcatc cattattgga catctataca tctaaattag
    30781 agcaaaatta tttcggtaaa cgattgaaca tggacttcaa caaacagctt gacagctttt
    30841 cattagaaaa tgaaatcgca gtactcgatt ataatatcaa attaaagaac tgcttcttca
    30901 gctaatagtt tcgagaaagt ccaaaaagga ggtatacatt gagatactac tttgtggatg
    30961 aagaaaagaa tgtaatagaa acagatgaaa aaaaatataa ttggctcgta cttgatagtg
    31021 caaagcccga agagattgag caagcaatcg aagaattcga gcttcctgat gatattttcg
    31081 taggaaccga atatccggaa gaagtttctc ggatggagcg cctttatgag acgaaactag
    31141 agcatccatt ttctttggta gtgattaatc tagatcagtc agataatcgt atcgaaaaaa
    31201 aattaacgcc aatatctttt gtactttccg ataatctctt gattacttgt atcgatagaa
    31261 aaaccgaatt tgtggatcgg ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa
    31321 agatcatcac gtatacatta ttagatattt acacccatta cgtgaaagaa ctaagagaga
    31381 tgaaaaagcg aattgacgct ttggataaag cagctcgtaa aacaacggaa aatgaagaac
    31441 tgtataaaca ggcagatctt gaaagagaca tcgtatatat cgatcatacc ttaagagatc
    31501 aacgggaaac aatggatcaa ttatggaatt ctgaaacttt catcaaacgt ttagacgatg
    31561 aacagctact ctatgatgtc catttgagac agcgacaaac agaaaaaatg atcgagatct
    31621 atcgagattt attagagagt attggtgatc tattcaacgg gatgatggac aataatctaa
    31681 atcatatcat gaaatatcta gattcagctg cattaatcat ttccgttccg gcgttgttcg
    31741 cagggatttg gggaatgaat acaggaggat taccgggtaa aagttctgct ctaggcttta
    31801 tactcgtagt ctttggctct ttgatagcag cagtagtttt aggttaccat ttataccgga
    31861 aagattatac aaattaaaga atctatttaa aacaggtatc aaacaaagaa atttgttttg
    31921 gcaatacaat ttaaaaacta ggaggaatac agcaatgaca gaaccaaaat taatcgaccc
    31981 gcgaaacttg taccatacgg agaaatttcc taaacaagat caggatacac cagctttaca
    32041 gcagaatatg attcctgttc ctgattgtgg agaagaatcc tatgaaggca ataatcaact
    32101 agaaaaccgc cgtgtactaa tcactggtgg agattcaggg atcggtcgtg cagcagcgat
    32161 tgcatttgct agagaagggg cagatatcgc tcttcaattc ttcccaggag aagaaaaaga
    32221 tgcagaggaa gtagcaaaat acgtgaaagt tgcaggacgt aagattgtct tactaccttt
    32281 tgatcttcgt gacgaacaag cacctaaaga aatcatagat aaagccgtga gtgaacttgg
    32341 cgggctagat acgatggtac taaacgcagg acagcaaatt tcttgtcagt ctattgctga
    32401 gttaccactt gagcaagtca aagacacatt tatgataaac atcatcgcta tgtttgcttt
    32461 agtgaaagaa gctgttccgc atatcccagc aggaggagca atcgtaacaa caacttctgt
    32521 tcaagcattc aatccaagcg aacatttatt agattacgca gcaacaaaag cttcaatcgc
    32581 taactttacg atcggtttag caaaacagct tgcacctaaa ggaatccgtg taaatggtgt
    32641 agcacctggt ccaatctgga caccattaca actagacgaa ggacaacctt ctgattcgat
    32701 ccctgaattt ggtcagcaaa gtttactaga acgtgcaggt cagccaggag aattagcacc
    32761 agtttatgtt ttcttagcat cgaataaagc aagttatgtt acagcccaaa tttacggagt
    32821 aactggtgga gaagcgatca acttataaga agttgatcgt ttcaccccta tgaggaggtg
    32881 cgaaaatgaa taaaggtgta aaaacgatcg gagtgatcgg aacattactt ttgctatctg
    32941 tgcttttgtt tgcgatacta ttaagtgcaa cctatccatt gccttttcag ctagagaagt
    33001 ttcgattctt ttccatcact aattcatacg tgcaacaata tgtattttgg cttgccgttg
    33061 catttgctat tttgacaatt atcacgttac ttgttttcct gttttatcca aaaacaatcg
    33121 gaaactttct actgaaacat ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag
    33181 gaatggttcg ttctcacttg catgaagatg aattcatcca ttcaccaaaa gtaaatgtca
    33241 aagcaacgaa aaacaagatc gccgtcaatg taaaagggga gttgaaacga acttcagcac
    33301 tcattggtaa aacaggaaca ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg
    33361 gaacaaaaga gccagtgaaa gtatcggtga aatacacagg ctttgaagaa ccagctaaac
    33421 gaagcaatga ccgcaaacga gtagaatagg gggggaccaa aaatgaaaga actattttcg
    33481 acatacaagc tccctatcat ctttggggta cttggattga tattagccgt tttatggatc
    33541 accgtgggct ttttcaagac cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc
    33601 ggtttttatc tgaaacaaac gaaaatctta gatcactatt ttaaatctta ggaggaaaca
    33661 tccatgaata acaataacaa aaataataac aataaaatga acaaccaaaa accagtgtct
    33721 gcacatccag acccaacgac accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa
    33781 acagcaccag tatctgcaca tccagatcca gtagtcccta acgttcaaca aggagtagaa
    33841 gagaagaaac atgatgcaga aaagcatgca cctcatcatc caggaactcc agaatctaaa
    33901 gttccagaac aacatgatgc aatcgatcct tctaaacctg tggaagagca aaaacatcct
    33961 tctgaccatg cgaaagcaca agaaaaactt agttctgacc acacaaacgg acaaggaaaa
    34021 cttggttctg atcatacaaa taaccaagga aagcatgatt ctgaacagga agattcaatg
    34081 cgcggtgagc tgaattatga agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa
    34141 caagtagacg gtttattatc tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt
    34201 gtgaatacag ataatgtaac ggctgggatc gaaacagaag tcggtaaaaa acaagtagca
    34261 gttgatatgg atgtcattgt ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg
    34321 caagaagtaa tcggcaaaga agtaaaaaat atgacacatt tagaagtgat cgaagtcaac
    34381 gctaacgtag tggacatcaa aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa
    34441 gacaaagtaa ctgacgcagc gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa
    34501 gtaaaatcag cagctggaag cggcgcacaa aaagtaaaag aaaatacaga accacgggtt
    34561 cagtaataag gaagtaaaac attaggagga acgaaacatg ggatttattt ggtcacttat
    34621 cgtcggagga attatcggag caattgcagg agcaatcacg aataaaggtt cttcgatggg
    34681 gattattgca aatgtcgtag caggtttagt tggatcagca cttggtcaag cattattagg
    34741 ttcttgggga ccaagtattg caggaatggc aatcgtgcca tcattgattg gtgccatcat
    34801 tttagtacta atcgtatcat tctttgctcg aaaactgtaa aaagactttt aaaaagaggt
    34861 cgtgaaaaag ctgttcttca tcaagtaata agaacagcca aacaaaaata gcttctcata
    34921 tttttcattt ggttgggcat attatcgaag atgcaagctt ttgaacactg tttatacgga
    34981 ataagaggct gtgacaagac ttttgtcaca gcctcttatt tatgcgggca aattcccaat
    35041 tagattttta aaagtttttc cttaaatgtg tctttattga tacagagaag cttctttaat
    35101 aaaacaatgc ctccctaaat tttcttgaga taggaaaggt gaacatacaa aataatacga
    35161 ccactttgaa aacccagatg atccacaaat ggatcgtatg atactgatga agtaaaaagc
    35221 aatggatcag aaactcaaag agattgaaaa agacaaggaa gaaataatat ctctttttta
    35281 ctcttgtctg ttttagccaa taatagctcg taaagtaatc tagacaaaca aatagaagta
    35341 aacaaagttt cgtaagagca gcaatcgtat catgttgctc tgctagacga ttgacgagca
    35401 ggaaaaatgt tggtaatgga atatagatca cgagtaatga aagaaaaaag taaacaataa
    35461 atggcatact attcctccta attgtgtgag ttcgccctca ttttaaaata acgatatttg
    35521 tttgtgaagc tttttcgtgt cgtaacaaat gttatctaat gtatttttaa ataaataatc
    35581 attattatca aaataatg
//
LOCUS       DACEKG010000025        34784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEKG010000025 DACEKG010000000
VERSION     DACEKG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.025"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(233..1081)
                     /locus_tag="IW664_001118"
     CDS             complement(233..1081)
                     /locus_tag="IW664_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0848495.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IW664_001119"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IW664_001119"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0848496.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IW664_001120"
     CDS             complement(3582..3761)
                     /locus_tag="IW664_001120"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0848497.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IW664_001121"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IW664_001121"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0848498.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IW664_001122"
     CDS             5909..8188
                     /locus_tag="IW664_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0848499.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IW664_001123"
     CDS             complement(8281..8928)
                     /locus_tag="IW664_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848500.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IW664_001124"
     CDS             complement(9107..9826)
                     /locus_tag="IW664_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0848501.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IW664_001125"
     CDS             complement(9957..11108)
                     /locus_tag="IW664_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0848502.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IW664_001126"
     CDS             complement(11325..12065)
                     /locus_tag="IW664_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0848503.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IW664_001127"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IW664_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0848504.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IW664_001128"
     CDS             complement(12449..13042)
                     /locus_tag="IW664_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0848505.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IW664_001129"
     CDS             complement(13026..13673)
                     /locus_tag="IW664_001129"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0848506.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IW664_001130"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IW664_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0848507.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IW664_001131"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IW664_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0848508.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IW664_001132"
     CDS             complement(15119..15646)
                     /locus_tag="IW664_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0848509.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IW664_001133"
     CDS             complement(15826..16611)
                     /locus_tag="IW664_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0848510.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IW664_001134"
     CDS             complement(16604..17473)
                     /locus_tag="IW664_001134"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0848511.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IW664_001135"
     CDS             complement(17474..18853)
                     /locus_tag="IW664_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0848512.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IW664_001136"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IW664_001136"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0848513.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IW664_001137"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IW664_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0848514.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IW664_001138"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IW664_001138"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0848515.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IW664_001139"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IW664_001139"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0848516.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IW664_001140"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IW664_001140"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0848517.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IW664_001141"
     CDS             complement(22811..23035)
                     /locus_tag="IW664_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0848518.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IW664_001142"
     CDS             complement(23088..24050)
                     /locus_tag="IW664_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0848519.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IW664_001143"
     CDS             complement(24060..24500)
                     /locus_tag="IW664_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0848520.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IW664_001144"
     CDS             24806..25771
                     /locus_tag="IW664_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0848521.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IW664_001145"
     CDS             complement(25828..26058)
                     /locus_tag="IW664_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0848522.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IW664_001146"
     CDS             complement(26144..27031)
                     /locus_tag="IW664_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0848523.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IW664_001147"
     CDS             complement(27120..28343)
                     /locus_tag="IW664_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0848524.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IW664_001148"
     CDS             complement(28360..29439)
                     /locus_tag="IW664_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0848525.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IW664_001149"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IW664_001149"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0848526.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IW664_001150"
     CDS             complement(31297..31563)
                     /locus_tag="IW664_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0848527.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IW664_001151"
     CDS             32042..34276
                     /locus_tag="IW664_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0848528.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="IW664_001152"
     CDS             34424..34744
                     /locus_tag="IW664_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0848529.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9842 a         7509 c         6060 g        11373 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt cagatatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggtagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaa
//
LOCUS       DACEKG010000026        33706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEKG010000026 DACEKG010000000
VERSION     DACEKG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.026"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW664_001153"
     CDS             4..816
                     /locus_tag="IW664_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0848530.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW664_001154"
     CDS             813..1613
                     /locus_tag="IW664_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0848531.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW664_001155"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW664_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0848532.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW664_001156"
     CDS             3110..4819
                     /locus_tag="IW664_001156"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0848533.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IW664_001157"
     CDS             5031..9383
                     /locus_tag="IW664_001157"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0848534.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IW664_001158"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IW664_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0848535.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IW664_001159"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IW664_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0848536.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IW664_001160"
     CDS             11221..11514
                     /locus_tag="IW664_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0848537.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IW664_001161"
     CDS             11511..11822
                     /locus_tag="IW664_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0848538.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IW664_001162"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IW664_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0848539.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IW664_001163"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IW664_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0848540.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IW664_001164"
     CDS             complement(14623..14844)
                     /locus_tag="IW664_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0848541.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="IW664_001165"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="IW664_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IW664_001166"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IW664_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0848542.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IW664_001167"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IW664_001167"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0848543.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="IW664_001168"
     CDS             20242..21027
                     /locus_tag="IW664_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0848544.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="IW664_001169"
     CDS             21040..21834
                     /locus_tag="IW664_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0848545.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="IW664_001170"
     CDS             complement(22014..22466)
                     /locus_tag="IW664_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848546.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22931..23299)
                     /locus_tag="IW664_001171"
     CDS             complement(22931..23299)
                     /locus_tag="IW664_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0848547.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23449..23931
                     /locus_tag="IW664_001172"
     CDS             23449..23931
                     /locus_tag="IW664_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0848548.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23968..24978
                     /locus_tag="IW664_001173"
     CDS             23968..24978
                     /locus_tag="IW664_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0848549.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25093..25806)
                     /locus_tag="IW664_001174"
     CDS             complement(25093..25806)
                     /locus_tag="IW664_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848550.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25996..26586)
                     /locus_tag="IW664_001175"
     CDS             complement(25996..26586)
                     /locus_tag="IW664_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0848551.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27070..27297
                     /locus_tag="IW664_001176"
     CDS             27070..27297
                     /locus_tag="IW664_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0848552.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27313..28479
                     /locus_tag="IW664_001177"
     CDS             27313..28479
                     /locus_tag="IW664_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0848553.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28713..29756
                     /gene="hrcA"
                     /locus_tag="IW664_001178"
     CDS             28713..29756
                     /gene="hrcA"
                     /locus_tag="IW664_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0848554.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29850..30413
                     /gene="grpE"
                     /locus_tag="IW664_001179"
     CDS             29850..30413
                     /gene="grpE"
                     /locus_tag="IW664_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0848555.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30464..32293
                     /gene="dnaK"
                     /locus_tag="IW664_001180"
     CDS             30464..32293
                     /gene="dnaK"
                     /locus_tag="IW664_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0848556.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW664_001181"
     CDS             32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW664_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0848557.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        11454 a         5812 c         7275 g         9165 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
    22981 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
    23041 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
    23101 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
    23161 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
    23221 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga
    23281 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
    23341 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
    23401 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
    23461 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
    23521 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
    23581 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
    23641 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
    23701 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
    23761 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
    23821 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
    23881 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
    23941 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
    24001 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
    24061 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
    24121 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
    24181 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
    24241 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
    24301 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
    24361 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
    24421 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
    24481 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
    24541 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
    24601 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
    24661 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
    24721 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
    24781 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
    24841 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
    24901 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
    24961 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
    25021 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
    25081 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
    25141 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
    25201 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
    25261 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
    25321 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
    25381 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
    25441 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
    25501 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
    25561 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
    25621 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
    25681 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
    25741 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
    25801 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
    25861 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
    25921 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
    25981 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
    26041 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
    26101 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
    26161 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
    26221 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
    26281 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
    26341 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
    26401 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
    26461 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
    26521 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
    26581 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
    26641 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
    26701 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
    26761 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
    26821 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
    26881 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
    26941 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
    27001 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
    27061 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
    27121 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
    27181 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
    27241 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
    27301 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
    27361 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
    27421 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
    27481 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
    27541 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
    27601 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
    27661 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
    27721 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
    27781 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
    27841 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
    27901 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
    27961 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
    28021 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
    28081 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
    28141 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
    28201 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
    28261 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
    28321 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
    28381 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
    28441 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
    28501 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
    28561 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
    28621 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
    28681 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
    28741 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
    28801 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
    28861 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
    28921 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
    28981 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
    29041 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
    29101 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
    29161 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
    29221 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
    29281 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
    29341 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
    29401 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
    29461 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
    29521 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
    29581 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
    29641 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
    29701 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
    29761 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
    29821 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
    29881 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
    29941 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
    30001 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
    30061 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
    30121 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
    30181 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
    30241 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
    30301 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
    30361 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
    30421 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
    30481 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
    30541 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
    30601 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
    30661 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
    30721 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
    30781 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
    30841 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
    30901 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
    30961 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
    31021 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
    31081 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
    31141 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
    31201 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
    31261 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
    31321 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
    31381 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
    31441 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
    31501 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
    31561 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
    31621 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
    31681 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
    31741 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
    31801 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
    31861 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
    31921 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
    31981 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
    32041 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
    32101 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
    32161 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
    32221 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
    32281 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
    32341 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
    32401 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
    32461 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
    32521 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
    32581 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
    32641 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
    32701 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
    32761 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
    32821 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
    32881 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
    32941 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
    33001 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
    33061 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
    33121 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
    33181 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
    33241 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
    33301 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
    33361 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
    33421 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
    33481 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
    33541 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
    33601 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
    33661 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaa
//
LOCUS       DACEKG010000027        32633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEKG010000027 DACEKG010000000
VERSION     DACEKG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.027"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            600..929
                     /locus_tag="IW664_001182"
     CDS             600..929
                     /locus_tag="IW664_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848558.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            916..1257
                     /locus_tag="IW664_001183"
     CDS             916..1257
                     /locus_tag="IW664_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0848559.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            1371..1610
                     /locus_tag="IW664_001184"
     CDS             1371..1610
                     /locus_tag="IW664_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848560.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            1677..1916
                     /locus_tag="IW664_001185"
     CDS             1677..1916
                     /locus_tag="IW664_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848561.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            2043..2732
                     /locus_tag="IW664_001186"
     CDS             2043..2732
                     /locus_tag="IW664_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ0848562.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            2738..3904
                     /locus_tag="IW664_001187"
     CDS             2738..3904
                     /locus_tag="IW664_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0848563.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            3986..4657
                     /locus_tag="IW664_001188"
     CDS             3986..4657
                     /locus_tag="IW664_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0848564.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            4710..6686
                     /locus_tag="IW664_001189"
     CDS             4710..6686
                     /locus_tag="IW664_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0848565.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            6699..7451
                     /locus_tag="IW664_001190"
     CDS             6699..7451
                     /locus_tag="IW664_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0848566.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7467..8228
                     /locus_tag="IW664_001191"
     CDS             7467..8228
                     /locus_tag="IW664_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848567.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            8241..8501
                     /locus_tag="IW664_001192"
     CDS             8241..8501
                     /locus_tag="IW664_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848568.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8498..10588
                     /locus_tag="IW664_001193"
     CDS             8498..10588
                     /locus_tag="IW664_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0848569.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(10624..12681)
                     /locus_tag="IW664_001194"
     CDS             complement(10624..12681)
                     /locus_tag="IW664_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0848570.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(12826..13017)
                     /locus_tag="IW664_001195"
     CDS             complement(12826..13017)
                     /locus_tag="IW664_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848571.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            13205..13660
                     /locus_tag="IW664_001196"
     CDS             13205..13660
                     /locus_tag="IW664_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848572.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            13657..13899
                     /locus_tag="IW664_001197"
     CDS             13657..13899
                     /locus_tag="IW664_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848573.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            13917..14219
                     /locus_tag="IW664_001198"
     CDS             13917..14219
                     /locus_tag="IW664_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848574.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            14288..16342
                     /locus_tag="IW664_001199"
     CDS             14288..16342
                     /locus_tag="IW664_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848575.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            16342..18954
                     /locus_tag="IW664_001200"
     CDS             16342..18954
                     /locus_tag="IW664_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0848576.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            18951..21329
                     /locus_tag="IW664_001201"
     CDS             18951..21329
                     /locus_tag="IW664_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848577.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            21390..21932
                     /locus_tag="IW664_001202"
     CDS             21390..21932
                     /locus_tag="IW664_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848578.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            22010..22342
                     /locus_tag="IW664_001203"
     CDS             22010..22342
                     /locus_tag="IW664_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848579.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            22343..22936
                     /locus_tag="IW664_001204"
     CDS             22343..22936
                     /locus_tag="IW664_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848580.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            22958..24982
                     /locus_tag="IW664_001205"
     CDS             22958..24982
                     /locus_tag="IW664_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0848581.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            24982..26181
                     /locus_tag="IW664_001206"
     CDS             24982..26181
                     /locus_tag="IW664_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0848582.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            26197..26841
                     /locus_tag="IW664_001207"
     CDS             26197..26841
                     /locus_tag="IW664_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848583.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            26852..27658
                     /locus_tag="IW664_001208"
     CDS             26852..27658
                     /locus_tag="IW664_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848584.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            27681..27910
                     /locus_tag="IW664_001209"
                     /pseudo
     CDS             27681..27910
                     /locus_tag="IW664_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28069..29004
                     /locus_tag="IW664_001210"
     CDS             28069..29004
                     /locus_tag="IW664_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ0848585.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            29048..29317
                     /locus_tag="IW664_001211"
     CDS             29048..29317
                     /locus_tag="IW664_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848586.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            29593..29868
                     /locus_tag="IW664_001212"
     CDS             29593..29868
                     /locus_tag="IW664_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848587.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            29910..30332
                     /locus_tag="IW664_001213"
     CDS             29910..30332
                     /locus_tag="IW664_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848588.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            30352..31944
                     /locus_tag="IW664_001214"
     CDS             30352..31944
                     /locus_tag="IW664_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848589.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            31965..32300
                     /locus_tag="IW664_001215"
     CDS             31965..32300
                     /locus_tag="IW664_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848590.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            32446..>32633
                     /locus_tag="IW664_001216"
     CDS             32446..>32633
                     /locus_tag="IW664_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848591.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11730 a         5027 c         6674 g         9202 t
ORIGIN      
        1 atcagctgaa ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat
       61 attgtcgaga cttctaaagg aacttagaat cactagaaaa ataaggagta gacttgatct
      121 atcaacaatt cccttcgggg agtgacgggg taggttttta cgattggtaa cgtaaaaaat
      181 tggtcaatcg agtaaatgcc cttgtttgga tagtggtgaa atgaatttgt gtaaataata
      241 tgcacagagt aaacaagagg gaatttagaa gttagacaag ttagaatagc gcctagatta
      301 atcatgagca gctactaagg gatctaggct ttgacgcaaa ctcatggaat aaggagtagg
      361 tcttctcgta atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat
      421 acttgcgagg acgttgatca ttggatcaat cgagcattta ccgctgtcta ctagggtttc
      481 tcgtttcgga cgccataaac gagtcgattt attttatata tttttgagtg aacgaaaaac
      541 gataaagata ttcgttatga agtaaaacta atttagtaga tagcaaaggt ggtagaggaa
      601 tgattcaaaa atttgcgaaa atgagtaaaa ctgcatggat tgaaaagctt gaaaatgact
      661 tgagtgtcat gcgattagct ccaaagaaat tcaaacgaga gattgcagaa aaagaagcaa
      721 ttttgtcggt attgaatgcg gtagacgata aatgtccagt aaaaattagt ttttcagata
      781 tagcaacaat tatcgaaggt atttattatg gtgaagttga tgaagagata tcttctacaa
      841 aagatttatt agattttttg gataaaaaca aagatacctt tatagtacta actgattatg
      901 aggaggttac aacaaatgaa aaattatgat ccgaatatac gattgggcac acatacgatt
      961 aaggtttctt tccaacgttg ggattacaaa ggatttgtga cgtttagaag aggcggaaat
     1021 tgtaaaggat tagatgtttt agcattagac gaggatgatt tatacgatca aacattgact
     1081 gataatccaa ttggattcgg cttgctacct gaagatgatg aaggtaatga atggtttaaa
     1141 atgacgctta cggatgataa tggagacgaa ttatctgttg aagatacttg gtcatatttg
     1201 agtgattata ttgtcagcgt tgaaatcatt gaatttgtag cggataaaga agaataaaaa
     1261 ctggaggaat aaattttaca gataaaggct tagaaggagt cacgacgaat aataaaaagg
     1321 tagtattacc ttaggcattg agtaaggaat ttatttagga ggaaaattag atggcgcgac
     1381 cagttttaga ggcgatggat atggatactt tgacaaaagg aaggtatgta cagctgttag
     1441 ctcgtggtta ttcacctaaa gatgtattca aatccctgtc taaaggaaca gatatggaaa
     1501 aagaaagatt acgaaaagaa ttcgattatt ggagaaccca taatggaatc aaggatttga
     1561 aaccagcacg gccagtaatt aaacgaaaga aaaagataac ccaagactga agtgtagagc
     1621 gtatgtgatc ataggctttt ttatttttgc aaaaaacaag aaaggtagga aaactcatga
     1681 attatgaaga aatcgaaaac agaaaaaaag tgtcgaaaga aatggaagaa aagttgctaa
     1741 aaatgatgaa acaaaaacac cttaaaagat tatctgtcat gcaatatatc aatgatatga
     1801 agataacagg aaaagagaaa gcttgtttgt taggttcaat gaaaaacttt gaacagttga
     1861 ggcgaactta tgtaaaaaca ggttccaatt gccaactatt attagaagtt tcttgacgat
     1921 tatgatatga aaatgatcaa ttatgatgtg gcattgttat tgaataaaaa gaatagtaaa
     1981 atttaacatg gtttatcttg gttagatgta ggtaaattaa ttcatatata gaaggtagat
     2041 agatgaataa aaacaataaa actttattta atccttacgt gggcgtaatc attgtttttc
     2101 ttctttttag ttattttgct tatcaaggtt atcaaaagga caaaatcgct gaaaaagtag
     2161 atggtgtaac aaaaacaatc catattcagg aacaggctaa aacctatcaa gataatttaa
     2221 ccagtttaac ggatatttct aagagacaac tgattactaa tcaaaaacag gcattagaaa
     2281 aggatctcaa aggtgaaatt gctggaaaac ttgttttttc ttctacagga tttattctcc
     2341 ctgtttttca aggagcgaat agcgacacgc ttagtttagg agtagcttct acttattatc
     2401 tagatactga aatgggaaat ggaaattata ttcttgctgg ttataacgta gatacaccaa
     2461 gcgttttatt atccgatatg actgatttat ctgaaggaga agaaattgat ctaatcgatg
     2521 ctcattacaa gtatcagtat gaagtctcca aaagaggaaa aatttctgat tacagccagg
     2581 tagttcaatc taaggaaaac aattcctttt tatccttgcc aaaagcaggt aaaaaaccac
     2641 tactcacact gttagccttt caagcaaata atatgaaaaa gtgcgatgtg atccagtgtg
     2701 aattagtcaa tgtagaaaaa atcaataaat aagggaagtg agtcacattc aagaacttgt
     2761 agaaaaattg atcaataaaa tgagtgccat ttttctgtta ctgttggtat ccattctata
     2821 tttaatatgg acggtggggt taaatcctcc tacgatatta tttatttatg ataatgctgc
     2881 acagttagtg acgtttttcg taggctgttt gtttcttttg aatctgtaca aggtaaagag
     2941 aaacgatcta ttcttcattg cagcaggcat tcttttttac cttttctttt ctttttttag
     3001 aagctaccga ggagatactt tctatggtga tctgatcgtt ccgacaatta ttgctattat
     3061 tttgattgta aaatggacga aatttactgt aattgatcgt agcttgtatc ttgtagtatt
     3121 ctatgtcttt ttactcgtaa cggtttatcg agtatttact gagataccag taccagaagg
     3181 acaaagtatt tggaatccat cgaacaaatt atcagatatt tggatcaata caaatacgat
     3241 aggtagttct ttaatgacat tagcccttct tatcacagga tttgctagtt catttggaaa
     3301 atggtacatg cgattgctat cattaccagc gttgattctt gctggggcaa cgatttgggt
     3361 ttgccaatcg aaagcagcat tatatgcgct tgttatcttc attatcttag aaattttacc
     3421 taagaaggtt tttcgaaata cacacttgtc cttttggatg tatgcagcag tttcggttct
     3481 agctttaccg atttcttatt ttgctgcaat gtcagacaaa gttcacttat ttacaggacg
     3541 agaagaaatt tggcgcaaat tctatgaaac attaggcgaa aaaacaggac aaatatttgt
     3601 agggatgaaa cctttctttt tccatagagg aaatcagatt ctaggtaatc ataatacgta
     3661 tcacgcactc gtcaatcact atggattgat tggttttgtt ctatcgatta ttcttttgct
     3721 tagtttctta tggttgctcg taagaaaacg tgagctgaca aatggacaac taacattctt
     3781 gtgggctttt ttagcgatca tgtgtcaatc gttcatggaa gaaacattaa cgacaatcag
     3841 ttgggtgcca attgtattct taatgttagc aatggctaca caccaatata aaaccaccag
     3901 ataaatgaaa tagaatagta ataagagaaa acactttttc gttgtgaaaa agtgtttttt
     3961 gttttgtctg aaaggaggca gatggatgaa aaaatggtta tttggttttt taggagtcgc
     4021 tttaatcgtc gtttgcagtg tgtttggcta tgttagctac caaaaacatg aaggagaggt
     4081 gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa atcaatgcac acgcaaagag
     4141 ttataaggag gatgcaacga acgtcaataa tgatatgtct ttggggcaaa tgttgtccat
     4201 acaaaaagaa gcaatcgaaa tgggcgtcaa taaacaagta tttgcacaaa ttcagattcc
     4261 tgcacttggt ttagcattgc caatttttaa aggggcaaat caatacactt tgagtcttgg
     4321 agcggctact tatttttatg aagatgccga aatgggtaaa ggaaactatg tcctagcagg
     4381 tcataacatg gaaatgccag gtgttttatt ttctgatatt caaaagttgt cattagggga
     4441 ggtgatggac ttagttagta acgatggcgt ctatcgttac aaggtaacgc gtaaatttat
     4501 cgtacctgag tactttaaat taatcgatgg cgttcctgaa gagaactctt tcttgtcttt
     4561 accaaagaaa ggagagaaac ccttattaac gttgtttact tgtgtttata cgtcacaagg
     4621 aaaagaaaga tacgtggtac agggagagct acaataacaa gaatttaatc aacgcgagtc
     4681 gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa tcggtatgta aaaaaagctg
     4741 gaatgattgt tggaatgtta ggaatcattg caggctgctt tcaggggtat cgagcttatg
     4801 cagaagatgt cacgcaaaaa acgccaccag aaagggtcaa tatcacggtt cataaattaa
     4861 tgtacgatca aagtacgcaa ttgaacgttg atattgacgg aatcaaaaat gatggttata
     4921 cgcacgatca atatcctaca ggtgtaacga aatacaacaa ggcagattat ggtgatgttg
     4981 agtttacgtt aggaaatatt acggatcaag tattaccgac tgaagattct gatctaacca
     5041 acgcgaaagt ggacgaaatc gttaaagatg tagaagacaa gggatctaac agtgagtacg
     5101 ttaagaatgc aaccaataaa attacttcag ctgtagatga aaatggtgag atcacttttg
     5161 ctgatcaacc agcctatgtc aattccaaag ggaatgtata tgtggtttat gaaagtaaat
     5221 ccgctgctgg attagtcaca caaaaagcaa aaccaatggt tgtgattgcg ccaatgacag
     5281 acaacacagg atcaggattt ttaaaagata ttcatttgta tccaaagaac attgtttcta
     5341 agttgagttt tgaattaacg aaatttggtg acgatggcac agctcaaagc aaacaaacac
     5401 ctttaaaagg ggcaaaattt gagttataca agggcgaacc tggaaaagga acaaaattag
     5461 gagagcttgt atcggatgat caaggaaagc tgactgcaac tgatttgacg ttaggaaaat
     5521 actactttgt ggaagttcct tcagaagttg tagttggatc agatcaagaa ccaacagcag
     5581 accaatattt gttaggcgca gacgctcgca acgatgcgca caataagtta acctttgaaa
     5641 ttacgaatga cggcgtgaca agtgatttaa aagcttccta cgtgaactat aaagcaccag
     5701 tgatcgataa aacagtgaca aatggaacag gagaagaaca ttctttccaa attggtgacg
     5761 cagtgaatta tcaaggtaca attcatatcc ctacagatat cgcaggtgga acagatggga
     5821 ttacagtaaa tggtgtaaaa agtgaaactt ctccttacag tgtatttaaa tggggcgaca
     5881 cagcaggtaa aggactttct tatgttgctg aaaaagcaga tattaaagta acaaataatg
     5941 atggttcagt cgtattaaaa gaaaatactg actataaaat cgaaaattct gatagtggtt
     6001 ttgtgatcga ttttatcgta aataatggac aagtaagtga tacagttgcg aatttacatg
     6061 gtcaagatct aaaaatgact tataacatgt ttgtgaatga tagtgctgaa atcgcaaatc
     6121 cattaacaaa tagcgtggat ttcgtttata acaataatcc atttaatcag gaagaacacc
     6181 acgaaaaaac gaaagctgac gttgtgactt atggcgcaaa attcttaaaa gtcgatagcg
     6241 gcctatttgg aacaggtatc aaagcaacac cgcttgaagg cgcagaattt gcagctaaga
     6301 atgcagaagg taagtactac ggtggactta ctgatacaga taaagatggt gtcaaagaag
     6361 cagtttgggt agacgatgta gcaaatgcag caatcctaaa atcagataaa gagggtcatt
     6421 ttgaaatcac tggtttaact gaaggagatt actcactgga agaaacaaaa gctcctgaaa
     6481 actatcaaaa attaacaaaa gagatctctt tcaaagtaga caaagattca tacaaagaag
     6541 aaaaccgaat cacgatcaaa aataatcaaa aagcttccgt accaatgact ggtagcaacg
     6601 gtttccaatc ttacgtattg atctcttgct tgttgcttgg ggcaggcgca atttctgcag
     6661 ttgtttactt taagaagaaa gcctagaagg atcagctaat gattatcaga caccctaaga
     6721 aaaagcgaat catgggtaaa tggatcattg ctttctggtt actttcagct gttggtgtct
     6781 tacttttgat gcctgctgaa gcgagcgtgg cgaaatacca acaaaatcag cagattgccg
     6841 caatcgatcg aacaggaacg gcagcagaaa cggattcttc tttggatgtt gctaagattg
     6901 agttaggcga tcctgttggt attcttacaa ttccaagtat ttctttaaaa ctaccaattt
     6961 atgacggaac ttccgacaaa attttagaga atggtgtagg gattacggaa ggaactggcg
     7021 atattacagg aggaaacggt aaaaatcctt taatcgcagg gcacagtgga ctttataaag
     7081 ataatctttt cgatgatttg ccttcagtta aaaagggaga aaagttttat atcaaagttg
     7141 atggtgagca gcacgcttat caaatcgatc ggattgagga agtgcaaaaa gacgaactac
     7201 aaagaaactt tgtcacttac ttagaaccaa atcccaatga agatcgagta acgctaatga
     7261 catgtacgcc taaggggatc aacacacacc gtttcctagt gtatgggaag cgagtaacct
     7321 ttacaaaatc agaattaaaa gatgaagaaa ataagaaaca aaaattatct tggaaatggc
     7381 ttttaggaag tactgttttt ctttcagtca tgatcattgg ttcgctattt gtttataaaa
     7441 agaaaaaata aaggagggta accaacatga gaaatagaaa atggctattt gtttgtgcag
     7501 ccgctctttt ttctttcact gcctacacac agttgacgtt tgcagacaga caagaaagca
     7561 cggatagcac agaacaaaca atccaaacgt cagatagcag cacgattcaa agcagcgatg
     7621 aaaccgtcga ttcgtcaaca gttgattctt cagaagaacc taaaattgct gaagacgatc
     7681 aaatagtcaa acagaccatt cacgttcaaa aaattatttc ttccgaacaa ttagacaacc
     7741 aggggcaaaa gcttgaaaaa caagaaggag taaatggcgc gaagttttct gtttatgatg
     7801 tatcggacat tttacaaaag atggatgtta aggatctcac tactgaccaa attgaatccc
     7861 aattaaaaga tcgagtgaaa aaactatcca gtgatcaact gaagctaatt tctaatggag
     7921 aaacaaagac aattgatcaa caaacaggtg tctttgaatt ctcggtagaa gtacaggcaa
     7981 atcagaaaca agcttattat attgtcaatg aatcaagtcc tgaaaacatt tctaatagcg
     8041 aggatatttt gctattaaca cctgtttctg ataaaaatgg tgaattctta aaggatgtgt
     8101 ggatttatcc taaatcagaa gcttcgcaac ctaaagaaga agtaaaaaaa atcgtatcta
     8161 ctggcgtaaa gaaaaacttc tttgagaatt gttgggattt tgttactcac ttgttttcta
     8221 gagattaagg aagaaataaa atgaaactga tcaaattatt attattcaat ttttcttgtt
     8281 taaccgcagt ctttttgtac tgcggtttat tattttctaa actatctatt ttgccaagct
     8341 accaactata cctaatgagg attttaaaca taccagctgc ttccatgccg ctagatatta
     8401 tggaaaagct cattaggaat ccattattta ctacgatcaa tttgatcgtt attgtcggaa
     8461 tttcactagt catttataaa aaagagggga tgaagggatg aattttaaac actcgtggac
     8521 aagacggtta aaatatgttt ttcccataat gatgatgtta ggtacattgt ttgggttaaa
     8581 aacagccaat gtctttgctg aaaaagttat tgtcgatccg tcaaatccaa tccagaacgt
     8641 aaagaataat tttattattc caaaagatgt tcctaatgca aaaacaaata ttactgtaaa
     8701 cggagcgtca aaagtttaca aatatcatgt tgatgccaac aaaggtggtt ttacagacga
     8761 ctacgtagga ttagtggagg gaaacaaaaa tatacgctat ccttctttcc aaaaagaata
     8821 cggtgaaaca aaccttgttt tagaaggcgt aacaaccaac accaaagtca atattgacta
     8881 tggaaaaatc ggtacgtata acgggaaaaa agtcaatatc aagctagttc tatctaatat
     8941 tcacttatac tcggatacgt tgccgtggaa tctactagat aacaactata caaaaactca
     9001 ttttcgagac gatggctata aaaatacgaa tggtgcaatg agtaaatcta aaaaaagaac
     9061 agtactttgg atctcagaca atcttttctc aggaattgtt taccattcaa cacaaatgaa
     9121 tgtgcagcta gtagccacat atgaggacgg tagccccgtt caattttcag gagatacatt
     9181 tatcagtttt aactctttga accctgcagg aggaaaaagc acggatctta aaggagaata
     9241 tgcccactat gacaaaatga atacgacaga ttggtatgtc agaaaagata cggttctttc
     9301 agaatttaaa tctttctaca acaatctgaa cgtggtaggt ggccacccag gaggaagctc
     9361 taagctgaca caagcggata atgatttcaa taatttgcat gataaattag gagatccgaa
     9421 gtttggtcaa ggtactgtgt cctttaaaat atctgaagca aatccgacgt ttgtgattgg
     9481 ttcttctaat gttcaaacct ggtttacttt atcttctgcg acaatcttta gtgttgttcc
     9541 tgatcaacct gaaaaaacag gggttgataa aaatggaaat aacgtgaacg ataaaatgtt
     9601 gcaagtagga gacacgattc aatatcgaat caaacagaag gtgaatcgat taggagtgga
     9661 tttactagcg gtttacgatc gatttgagtt aatcgataat ttaccaaaag aagtaaatta
     9721 cgtcgatgca cacgttgaaa gtggtacaaa taaaaaattc gatgtatctg gtgaagtgac
     9781 ctatgacaaa acaaaacacc aggtaaaata tgcagcgaaa gctgatacat taaaaaatcg
     9841 aatgaagtat aacggcgaaa cttacgaatt agtgatcaat gtaaaagtca atgaattggc
     9901 caatcaaaat agtgtagcca aaaatcaggg aacgtcgatc attaataaag tagaaaagga
     9961 tacaaatatc attaccgttt atttccctaa aattccagtg aaagaagtac aacaaaatgg
    10021 aaaagatgta aacggacgaa atgacggtaa gaagggtacg ccgacagctc ctttaaacgc
    10081 aggatctgaa gtacaatatc tagtcacaca aaaatggcac acaaaaggcg tagacgctgc
    10141 gtcagatcac tacaaacaat ttagtattca agatccaatt gaagctcgtt tgacatataa
    10201 agaaggttct gcgcaagtaa tagataagag taaaggaaaa gatattactt ctgaaggcac
    10261 actaacctat gatagcaatt caagaacgct gaagtgggaa gcttccgctg acttcttaaa
    10321 taacaatctt ttagatggac gagaaattca gctgatattt acagctaaaa ctccattaca
    10381 atcagaaaaa aatattgata accaagccgt agtagcagta gataatgttt ctaataaaac
    10441 aaatgttgtg acaattgggg ttgatcctaa cttgccacaa gttattgttc ctaaaacagg
    10501 ttctacacat ttagtaacga ttttagcggt cagcttacta ttacttgtgt tagcaacttt
    10561 tggctacgca gttctgaagt tcaattaaac aaaaaaacaa ggctgaaaat cagccttgtt
    10621 tttctatttg aaaatgggat tggcactaat aaatacgttg tctgaagaaa gtgatccgtc
    10681 tgcagaaact tcataagcat gggataaacc aacgatactt tgattgttgt aaagcaagtt
    10741 tgcgttatga gcagcgctat ctttccacat aaccatagca tttactgctc ctgtatctga
    10801 aaggatctcg tcaacgtaaa ggtagaaact attgatattt tctcctttag caccgtagca
    10861 gcctaagctt tctaagagat cttggaaagt accgccgcca ggtcggtaat gactgaagct
    10921 tacttgtaat tcttttgcac gaatatctgc agcttgttga acgatatcat tgaattgcat
    10981 tggggcaacg ccattttgtt gacggtagct atttaaccaa gcaagttctt tttgacgcac
    11041 atagttgttt aaatcactta gtgagatatt tttgtatttg aaagtgatcg ttcgattttg
    11101 gtcaacatta ttgattacac gttgtccatt tttatcaacg gtaacagatg aatcgtcatt
    11161 tctacacaag aagtagtttt gatcggtcat atttttccaa gaagtagaga aactatctcc
    11221 ctcgtaaacc gtttgtgttt cagtagataa aacagcgttt gtgtcaacgt ttatatggtt
    11281 cacagtgacg ttgtattgat gagtataggc aaaattgatt ccttgattac ttgtaacgtt
    11341 gtttaaagtc acagtgttgc tagagctttc tctaggatca agaacaaaat tagtgaacat
    11401 aggtgcttca attgtgatgc tttgtccata aacgacggga taactcttac tgtaattata
    11461 gagttcttgg atatttggat cagtatccga gactttattt ggacgagctt tcacttcaac
    11521 tagaaagact tcatcatagt aaaaagtaaa tgtggtattt gtgttaatcg tttgtgtttg
    11581 tgtttcattc ccatttactt gatactgtgg tgcttcaaag ttagtcaagt cggttctagc
    11641 ttttgcagtg taacttgtac cagcttttac tgagacatgt tcttctttta gcagcgttcc
    11701 agtttgtcga ttcatatgac ggacagttac cgcatattct gaaggtttag gtttgtgttg
    11761 taacccttta aatgcagaat caagactatt tttagcatta tttacttggg tttgagaagc
    11821 tttagaatcg tctagtactt ttttagcatt ggataaagca gtctgaaaat tattccaaga
    11881 tccttctgta aaatctcctt tagcagttga ttttacttgg ttgtacaagg cttgtaattc
    11941 cgttttgttc acagtaggag ctggtttttt ctttaatccc ttgtaagcag aatctaatgt
    12001 gtttttggca ctatccactt gtttttgagt cgcttttgag tcatttaaca cttttttagc
    12061 attgtttaaa gcggtttgga atgcgttcca ggtattgtcg gtatagtcac cttttttagt
    12121 tcccttaacg ctcgtataaa gagcttctaa agctgattta ttcactttgg gtttagaggt
    12181 gtcagcacca taccaaccaa taccttcgta tttccagcca tagtctttgg ttaaccattg
    12241 tacttctttc caacttgcag tgtagtgatg cgaaccagag gtaacacctg gaaggaaaag
    12301 gcggtagatc ggtttattat ttttttcggc agaacgccag gcaggccctt cataagtcca
    12361 accgtaattt ttggtcaacc atttcacttc atcccaacta gctgtgtaat ggtgatcacg
    12421 aaggccttta ttgtataaac gatagacaag gtggccaccg ctagtcggtg cttcccaagc
    12481 ggggccttca taattacccc acttaatttt cactaggtga tcacgttctt ttgtgctaga
    12541 ggtgtaaaaa tgttcatggt tatttggatt ataaaggcga tacatgacgt tagttgaagc
    12601 agcgtcagca gatccagcaa gagcaactgt ggcagcaaat gttccaatag ctataacagt
    12661 ttttgggttt aataatttca tgttttcaac tccctattaa atgattaaat attttgttta
    12721 cattcataat ataccacggt ataccgaggt agtcaacggt ataccgtgga aattttgaaa
    12781 taaaaaagcc gccctatgaa gagtgtaagg atggctgttt atatgctatg aagaaatgga
    12841 ttcggcgtat ttggttagtt tggcagcatt atcaaatcgc attttttcaa tcgcactttt
    12901 tttctttatt aaatccgcaa ttgttgattc tgcgactgaa gaatgtttgc taatttcata
    12961 tgctgtcttg ccagtttcaa atagtaacca ttcgattttc tctttatcag cgtacattgc
    13021 aatgctccct cttgattttt tctttattat agctcttttt tgtaataaat cacaatatca
    13081 agaggaaaag tttctataga aaggtgggaa attatgagta tccgttcccc gtgtatcaaa
    13141 atgaggtttg ttgctacttt cgttatttaa tatactaaaa taaaacaacg aaagaaggaa
    13201 tagaatgaaa aagttaataa tggtatttac ttcgttgatt gccatgattg ttattttagg
    13261 tggatgtggt agtgaaaaaa ataagcaaga tagtcaagat aatggcgtgt taaaaccagg
    13321 aacagtgtgg aaagaagaag taggcggact tgttcataat ttaaaaatta tagatgatac
    13381 aacatgggaa tattccgaaa gtgtttggca tcctgaacaa gtacaaataa cagttgagaa
    13441 gctaaaagac tataaaggat tagaaagata taaggtaatc gattcaggtg gagtagattt
    13501 attcataggt aaaacagcct taatagttcc ctataacaaa gacggtttag aaacgtttaa
    13561 atttattcca acaaacgaaa aagaacatcg agataaaagt gaaatattaa aagaatacag
    13621 tactaaatct ggatacaagt tacaaaagac tagcgagtga ttgctagtct ttttatttat
    13681 cctgaaggag gaatcgcagt ggcaaaagct aaaacgccat ttgaaaaagc cgcgtctaaa
    13741 atcttagaac gtcgcggtgt atctgaaaca cagtggaaac aagaagtcct tgaaaaagca
    13801 caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta tcaacgtgaa
    13861 cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac aattttatgt
    13921 ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg gttgatattt
    13981 ttagagcatt acaaggtgca gcaactagtt taaaaaacaa tctgatgatt tttgcggctg
    14041 tagctgccgt tgcctccatt gttcttgggg ggattttctt cttctttggc cgaagaggag
    14101 ctgaaaacgg gaagatgcac gtaggttatg tattgatggg ggtaggagtt atattagctg
    14161 ccgcctctat tgtggtttcg ttatttggaa tgtttggaca aaccccacag ggactataaa
    14221 aaataaatag aaggagtaaa aagtacgtgg ctacttttta ctcctttttt atgaaaggag
    14281 aatactcatg tactttacga tttcaggcaa taccaaaaaa gataaagagt taataaaaaa
    14341 agcaacgaag aaagtgggaa agcgtactgc ctttttaccg aaaacaatta aatattctgt
    14401 cattgaaacg gaagaacagc tacatgtgtt aggacaagtg ttagaacaag agaatcaatc
    14461 ttctgttaaa ttgactctca cgttacatga taaagaagga aaaatagaaa aggctggcac
    14521 agaatacgtt tttggcccac tggattatca aatttcagaa ggtttttatc ctacattgat
    14581 ggatttgatc cgccttgatg tgtttaagga aacggatgaa accatgagtg tggaagagtc
    14641 ttatcgaaac gaaaaactgc tacaagtttt agaagaagag ttagagagcg accaaagaaa
    14701 agtgagtcaa gtgagtgaag tcgaagtaaa tgatgaagta ccatgggaac gaccaatctt
    14761 tccaaagaag gagtcaaaag aaaaagagac agtctatgtt gacgaacaag ctgatattcc
    14821 gactcagaaa tcgatatttg aggaagagaa cgatgtaatg ggtcaattgg aggtagatcc
    14881 ctttgaagaa acaatggagg atgaagagac ggccgaagta gaagttgatc tagttgaagc
    14941 taatgaagag ccaacatgga ctgaagggga ttctcaggaa tatattgaac cacttatcag
    15001 tgaagaagta gagaaaaacg aaacagagat acaaaaggca actgtatttg attatatttt
    15061 ggatcaatat ccgccagagc atgaatggat gaaagaaaaa aataaattat ttttaaatga
    15121 gctttattcg aaatatcaat taccccaaac ctatcaagca tttttagaga gtaaagaaga
    15181 attaatctca cagggtaaag gcacactttt agaacagtta gagtctgttc aatcaacaaa
    15241 ttggcatgag cttgcacaag aagatttatt agccactttt gaagaacgtc aacaagaaac
    15301 agatcaagaa ataaatgagt atgcgacgga acagtcatct aattgggaga gaacaaaaga
    15361 ggaactaaat aaagaagaac aacgagtgat tgaagaagaa gtacaaaaaa ttaaagagcg
    15421 atacgaaaag aaacgcgaca atgcgtattc cttgtgtata gagaaaatta ataactttac
    15481 taaaactaat caagaagctt tatcgaaaga aaaagaaaac ttgttaaaag aacgtataaa
    15541 agaacgaaaa gaagagttct accgaggttt acgtacagat aagctaaaga ttagcaaaga
    15601 attaaatgaa caactgaata aactttatca aacaacgact gaagttctta tggaaagaca
    15661 cgggcagatc cagcaagaat tacaagcaca aattccaaaa tggaaaaaag agtatgaaat
    15721 tcaacaacaa gaattgaaag aacaagaaga aaaacaacga caagaagaaa aagagcgcga
    15781 acaattagaa ctaaaaaaac gtgagcagca tcttgcagaa caaaaagtgg cgatagaaaa
    15841 acaacggtta gagcaagaaa aggaaaaaga agcagctgct cgtaaggaac gtcaagaaca
    15901 aatggataca ttgcgacaag cacagttgaa ttatctagca caacctcaac cagtagctgc
    15961 tgttcaaacg ccagtgaaca accaagcaaa tttaactcct tcaccacagc gttcctttaa
    16021 cggatggatg gtgggatgta ttgcaagttt aagcttgctt ctaggtggag gaacagtatt
    16081 tgcttttaat catatcaatc aatcttcaca ggcagattct gtagccacaa cggcgtcaat
    16141 caaggaagaa gcccaggaac aagcaagaaa agagtatgaa caacaattgg aagaattatt
    16201 gaatcgtacc aatcagtcaa cagcagagtc aagtatggaa aatagcaaag agagtgatcc
    16261 tagtaaggct aaagaaggat caagcgattc aactgtacac tcaaacactt ccgaaagttc
    16321 agaatcaaat gaggtgaaat aatgcgtatt agagaaacaa cgagacgaaa agacaaacga
    16381 ttcaatttag aaaagaatag gtatctttct catttctatc gctatactcc ttcaggagaa
    16441 gagctaccag aaaaacatga caaacgtatt tctcaaaaga ttttttatag tggcttagct
    16501 tatcttcctt taaactatgt tgttacaaga ataccagaag catttcatcg atttgagatt
    16561 attccaggaa gtttacaagg agtgaatatt tttcagttac aagaaatgcc gcagttattc
    16621 cattttgatt caactggttt ttggaatcct ttgaccccaa caacaagttt aggcacacgt
    16681 ttagttgttt ctacgatttg tgttgttcca gctgtactta tgggattgaa aatggacatg
    16741 tggtttcgtc cgctagcaga tgggcaaaaa ggagataacc gattaaaaac gttaaaggaa
    16801 atccaacaaa tttatccgca aataccagaa tcaaagggaa gttttcctgg ttatggcgga
    16861 ataccaatta ctcattataa aaaatactgg tatattcaga cggatacagt aaatacttgt
    16921 attgttggta cttcccgctc agggaaaggc cagatagaag ttttagcaac cattgacaat
    16981 ttgtcgcgtg cagaaagaca aagttcaatg gttgttaatg atccaaaaac agaattatat
    17041 gttgctagta aagatgaact agaagctaga gggtatgatg tgtatgcctt taatatcacc
    17101 gatccactgc aatcaatgag tgataatcca ttggcactca ttgtgaaata ctggaagcga
    17161 ggagacatag acaccgcaac acagttaacc aatactttta cgaatacgat ttattatgat
    17221 gcaaatgctt cagacaataa atactttaac gaaaatgcgc agaaagctgt caatgcgctt
    17281 atttttgcgt tattggaaca agcggatcaa acaggggatt actccaaatt aacaccaaat
    17341 aatctcgttg aactactttc agaaattggc ggttttaatt attatccaga tcctgagaat
    17401 gaattcaagc agctcaatgc cttagatgag ttcatgaatc agttaccccc tggaaatata
    17461 gcaaaaaaac aatatggggc aacaaaaatt ggatctgata aagcgaaagg gaatatcttg
    17521 tctacggcaa ttacaggatt gaatccattt accctaacga aaattgcgaa gatgacttct
    17581 caatcgacca ttgattacaa gtccattggt tttccgaagt atttggatct caaattacat
    17641 gaaagcctat taaatcaacg cattgtcgtg gaattttatc ataagggaaa gaaatttcac
    17701 gaggaacgag tcaaagttgg ctatcgtggt ttttgtgaaa tcaactttga ttgtcattta
    17761 gagcagggag atcatgtttg cttaaaatac aatcatcaag gaaaacaata tgttgcttgg
    17821 tatgagtttt ctcaaaaagt attgaaagac gaagagggaa aaatcgttta tcgaaaaaaa
    17881 cgaggagaaa ctcatttgcc agaactagaa aaagaagccg ttctaacatt agatgagaag
    17941 aaaagtaacc tattagtaga gtctattcgg atgcactata ccgataaacc aatggctatt
    18001 tttatgttaa cgcctgatta tgatccatct aaccatatca ttgttagcat ctttcttagt
    18061 cagttgtata aagaattatc tacgcaatgt gttaagacta aaggagataa atgtcaccgt
    18121 aggattcatt ttattttaga tgaatttgga aatatgccac cgatggacaa catggaagga
    18181 atcatgacgg ttacggcagg tcgtaatatg ttatgggatc tgtttattca atcttatcag
    18241 caactttatg cgaagtatgg agaacaaaac gcagcgatta tcaaagaaaa ttgtcagaca
    18301 catatttata ttatgtcgac agatgataat accatcgaag aattttctaa aaaagtgggg
    18361 aataagacgg ttgaacaaga gaacagtacc atcaattcat taggtatgaa tactcatatc
    18421 aatcgcaatg ttgattcaga tcgtatctta actcctgaaa gaatttctac ttttttagaa
    18481 ggtgaaacga ttgtactcgc tcctttaaaa cgacaagatt tgaaaggtca taaaatacgg
    18541 ccgtatccta tttttaatca tggggaaacg aatcttcctt atgcttatca atatattgaa
    18601 gatttcaatc catcgtggga tatcaatgat atagacatac attgtacaca tgcggacttg
    18661 gatcttaagg ctttacaaat tgattatgat tatttcctac cagatgaata tgctagaaat
    18721 ctctatcatg gactgcatga tcaattagaa gaaaaaaacg aagaaaatat gttttataaa
    18781 atcgttcaat cttttgagaa tgagacggct aaaaaattgt taattcaatt ttacgaagaa
    18841 caagcaacgg atcgtcttca agaatatgtt gaaaagttga tcaaaaatga agaaatttct
    18901 gaaatgatta ggaaacggct gttattggaa gctgaaaagg cggtgaaaga atgatcattg
    18961 gaggaatttt ctctggcata gccgactggt ggtcaggttt attcggagaa ggggatgcag
    19021 atcccaaaac cgtaacgaaa gtcttacaag attatggtaa ttatctgcag tatcaagatg
    19081 tattcagttg gatttgtaat acttttttat ggggattgat caaagggtta tataatctca
    19141 atactttatt agaaaaaaca atttatcaaa gctttgattt aaaagactta ttgaacgccg
    19201 ctggtgttaa tgaactattt caaagtttga ttagtaaggt attggctctt ttttgtgtcg
    19261 tcacattaat ttatttaggc ttgaaatttt ttatgagtaa acacccaccg aaagtcaaaa
    19321 atatcttggt taatttattt atggccatga tcttgatttt tggtggtagt tctttaatcg
    19381 atgaagggtt aaatatcagt aaaaactttt atggagacgt cactgcagca aataaagcag
    19441 ataaaacggg ttcacctgct tttcaaatta ttcaaaataa tgtgattgat gtcagtacat
    19501 tactagcgac tgacccgaat aaagtagaat ccttaccaac agataaaaga aatgctttaa
    19561 cagcgaaaaa tttcaaaaca gcgaatatca atgggattat tacacctgat caagctaaga
    19621 aaatgtctga atcaagtgat attagtacag tacaaaaaga tcggttaaag gcattacagt
    19681 atcgactaga gttagacgat aatgggaaag aaatccctgt tcaaattagt gatgaaggtt
    19741 tagcaaaata tgtgtatacg tcaggctatc gtcgatacat tagtagtcca gggattattt
    19801 tggctgggga atcttctttg tcggtagcct atttatttat tttgtttaca atcgttactt
    19861 gcattattga gctagtattt aaaaaattct atttagtaat tgccgcgtca acggactttg
    19921 agacaggaca acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg
    19981 cgtttacgat tttagaatta cgaatctata cattaatgct ttcaggtatc ggtgatttgc
    20041 atgcggtagg aaagatcaat ggattcttgt atgttgttgc tttaattgtg ctaactattg
    20101 cgctattcaa aggctctcaa tcggttacga agattttcgg tgttgatacg agtctgaaaa
    20161 acggcagcaa ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta
    20221 gcggagctaa aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc
    20281 gtaagaatgg atttggccat cgaaaagata ttacccatga taacggggaa gaacaatcag
    20341 acgataaagc tacaactgta cctaatagta agaagacagt ttccgaaatg ttcgggaaag
    20401 cgaaaaatgg tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa
    20461 aaggaacagt tgaagatctt acagaaaaag ccaaagacac cttagaagag aaattagacg
    20521 gcacgagagc taaagaattt gctgaagctg tgaagaatcc atccgaagca ctagaaaaac
    20581 aaaaagaaaa agcttcagca gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag
    20641 tcaatgcagc agtgaaggca aatacaacaa gtgaaaaaga aaatcaagaa ggtgcagcga
    20701 ttccttcagc caaagatcac caagatgagt tacattctaa cgaaaattcg atcggagaaa
    20761 aagattcaga agaaggacaa catggtcagt taccaaaaac cgatccagaa caatatcgtt
    20821 ataaatctaa cattaattct gaaaacgaaa aacgcatttc agatttacca aaacgaaatc
    20881 aagaagtaca aacggatgag gattccatcg atctgccaaa tgataatggc atcgaagttg
    20941 gaactgaaaa atcaacgaaa attggtttgc ctaagacaca aaatttacca atgaaagata
    21001 gtcgtttgaa tcaattcaaa gagaatgatc aaagctttgt tcatcaatca cctgcaggta
    21061 ctgttcaaat gaaatcaagt cgtttgcctg aaaatggaaa tctgacaact gtttcttaca
    21121 atttagctga aagtggtgaa acagagaatg cgtccgttca aaatattgtg acaaaagaag
    21181 ctgcacctaa gccaatcaat aaagttcata atggatacag accagcaaac gtagcattta
    21241 ctgcaccgac aaaaataaat gtgacaagag aaactaaaaa caggaacaat gaattgaaag
    21301 aacaactatt gaaggatatc aaaccataaa aggcctattt aagggccttt ttctttataa
    21361 aacaagttta aaatggaaag gagaaaattg tggaggatat tttaatagct acgtttataa
    21421 ctgcactggg agcattttta acgatatttg tggtgactat taaaggattt aaagatacgg
    21481 atagtttaca aaaagatcat cagtctattt tagatggtca aaaaacacat accgaaagca
    21541 ttattgtaca cctttcagaa ggattcactt atttgaaaga tgctatgccc acattcacag
    21601 taaaattaac ggaaatattt cattctgttg agcaaatatc gtcagaattg aaagagcaaa
    21661 gacaacaaaa agatcgtcaa cttggctcac ttaattttag gcaagaaacg atacaagaat
    21721 cattggagaa tgtaaatcga ttaaacgatg aaatgcaacg agttcatttg gaaaatgaag
    21781 aattacgcgc acaaaattat gatttaattc aaaagaaaag aaatctggaa caaaaaattg
    21841 ttgaattaaa agaagaaaat cgtcaattga attatcaatt acgacgagga aaaaatcgtt
    21901 ctcgtgattt aggaaatgaa ttagagttgt aatatttaga aaaacgctgt gagaaatcac
    21961 agtgtttttt atttctaaaa atacaaaata taaggggtat ggttatatta tggataaata
    22021 cattcatatg aaaaatcctc caagatttag tggggaatgg aagtttttcg cctttttctt
    22081 tttagatcta ttaacgcttg gtggctgctt tttttgggcg aaaatagtca ataatcttgt
    22141 ccacttacca ctcttttttg cactcttgaa ttatggatta tggcttgcga tagcgttagt
    22201 gattgtttgg cgaccctata ataaccctgg tcaacgccaa atttttgcga ttatcgaatc
    22261 tattttcttt acggataaga accactatca tgcgttagat cctaaccgaa attatcatgg
    22321 tacgtataag gaggatgaat aaatgcgagt caaaggacaa aattcagttg ataagaaatc
    22381 cattttgcag tttggaaaac cagaagagcg ctctatcttg gacttatttc catataaatc
    22441 ctgtacagaa gagggagtct taattactag agaagatcat tttcagcgtt attatcgtgt
    22501 cgtttccact gacgtggagg gattaaatga acaagagaaa ctagaacgaa tgaatcagtt
    22561 gaccaccgtc atgcgtacgt atgtgccaac aattaagtta gttagtttga ccacagagac
    22621 ggatctaacg gaacaaatcg tgcaaaagcg gcagttatta gataaaaatc gtatggaaca
    22681 gtcactagga agaaatctac agcgattaag aaaatatgaa aagaaattag tagaggagat
    22741 ccaggaatta aaacgggcag aacaagaacg tcctgactta agcttctttt tcgttattga
    22801 aggaaagaca atcaaggatt tacaaactag aaatcgtcag ttaatgcgat ctagtggcat
    22861 tttagggtta aaaccactct acaaaaaaga attgattaag attctttatc gaatgaacaa
    22921 catgaatgac gaataaaaaa gatggaggaa atgattcatg ctagatgtga taaaaacagc
    22981 tttttcaacg caaagtaatc aaaaggtgat tgatccatta aaacaacttg gtaatcgtga
    23041 aatagagatt ttaaaatcaa aaggttatga tttagatttc ttaaaacaag tgatgccaca
    23101 aggtgggttg aattttgatg aagaatatgt gacctatgga aatggttatt cacgatgtgt
    23161 gacagtttat cattacacaa aaaatcctac tcttttttgg ttggccaatc tcatgaacaa
    23221 tcctcataca gtggccacac tagatgttag cacagatgaa aagcaaaaag tgattgatca
    23281 aatcaaccgc gcattagatg aattaaatga tcgatcagat aatgaacgaa aagcgactga
    23341 ccgaaataaa agtgcggaag aatacctgga actagcggaa tatgcccgaa aattgacttc
    23401 taatggggaa atttctaaac aagtcaaagt gagagtacat gtttacggaa atacgatcca
    23461 agaagtggat gaacgggcgc aggaactgat cgaaaatcta aaagggatgg attatcgtgc
    23521 ggtgacttat ctgcataaaa ccaaacgaga ttggcaatcg atgtttacca tgttcgatga
    23581 acaagctgaa tggtttggaa atatcaaagg gcaatcactt ccatcgttaa acattggcgg
    23641 aggattcccg ttccaccacc aaacgctaaa agatccagga ggcacacaat atggggaaac
    23701 catgacaggc ggcccatttg tctggaatgc cacgtataaa aatagtgtac gtttatctac
    23761 caataccttg cttctaggaa tgatgggagc tggtaaatcg accgttctta aaatgatcgc
    23821 ggaagcccat ttagctgcag gagactttat ttggggcttt gaaaagggaa aagactttat
    23881 tcctttctta aaggaatata acggtattat ggtgcgttta gacggatctg acgggatgat
    23941 caatccttta gagatctttg ccacacggac gtatgatgaa gcatccagtt tatatgacga
    24001 tggttcagtc aaagatttga agatcaatga agcagcgtcc tatcaaacgc acttagacaa
    24061 agtagtctat caggtacagt tagtgagtcc tcagctaaaa ggaacgatga aagcagaatt
    24121 caagacctac ttgaatcgtt tttatgaaga gtatggaaca gtaccacgcg gttttacttc
    24181 ctcaaattcg cgttcaaata ctgatacgca agtcacaggg aaagatccag aagcttatcc
    24241 gacgttcaaa gaatttttag cctttttagg acaactggaa ctaccagggg cttcccaaga
    24301 gaaaaagaat cggaaagaag aaatggagtc tatcgtggaa tccttatgtg agacttatgg
    24361 catgattttt gatggtcata gtacgattcg acaccttgac caacaacagt tggtcttttt
    24421 tgacattgac tctattacag gcctagatcg aggggtacaa caatgtcaga tgtacatggc
    24481 catgacgttg atttggaatc aggcattgat tcaaggacga agacaacgac gcttaattaa
    24541 agaaaaacaa atggaagaac aagacttcaa acgcttcttg gcgatgattg atgagtgcca
    24601 taatgcgatt aatccagatt tcattgaaac ggtaaattat gtcacaaact tccaacgaga
    24661 aatgcgtaaa gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc
    24721 agaaaatatg tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac
    24781 gaagatcttt atgcgtatgg atgaatctat tttacgtcat atagaaggat tagttggtaa
    24841 atcaatgacg aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat
    24901 caattttggc tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa
    24961 attgtttgat ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa acagtatgtt
    25021 aagcgtcgtg caaagcggcg cttatttttg tgggtgtttg gtacttctgc aggtttgatt
    25081 actatattga tcactgtgtt tgtgaccctt tttctaatac ttgcggcagg ttctattgat
    25141 aattctgatt caagtagtgg tggcgaagct tttacaggag aatattcgga aggcttaccc
    25201 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgctgta
    25261 ggtgcagcag tgaagtataa attattacca agtgtgatcc tttcgcaata cggttatgaa
    25321 tcagcgtttg gtacgtcggc gtccgcaaga aatgatctca attattttgg gattacgtgg
    25381 tttgatgggt gtctgtttcc taaaggaact gcgcgtggaa ttggtggcat tgaaggtggc
    25441 tggtacatga agtttcccaa ctcaaaagct gccttctcct attacggctt tatggtagcc
    25501 acacaatcaa attttaatgc ttgtgtcgga aataaaagcc ctggcgcaag cttattgatc
    25561 ttaggacgag gagggtatgc cgccgcagga attactgaag acagtgccta ttataaaaat
    25621 tgtatgtcta ttattaatac aaataaattg atggagtacg atgagtttgc gattaaacac
    25681 tggagagaag gcggcggaac tggtggtacg attacaggaa gctggggcaa tccattccca
    25741 ggaagtagct tagataaaaa ttcgttcagt ggaggacaac tttttgggaa aaatccagga
    25801 ggagaattcc gacctaacgg gttccatgat ggactagact ttggttcagt cgatcatcca
    25861 ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt
    25921 tcaggtttgg gtgcgtgtgt gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa
    25981 gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt gaaagttggt
    26041 caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg aatggattgg
    26101 cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc gttaccattt
    26161 ttgaatagca gcaaaaaata gatcaggaga aaaaatatgg aaaataaaac cttaaaaatc
    26221 gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt ttggcagctt
    26281 aaagaagaac aagagacaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgat
    26341 aagctcaaaa aaaattcttc tgaagcacag gcgaagattg acaagttaaa tcaaaccatc
    26401 aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa agcaaaagag
    26461 gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
    26521 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
    26581 gacaaattaa gtcctgtaga cgatgatcac caacatgatg attacggaag ttactctttt
    26641 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
    26701 gtcgtatttg ttgattatac aatcggcaat cctcaattca aagatgtcaa accacaaaaa
    26761 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcacagac
    26821 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag caagacaacg
    26881 ttttaaaaaa cgaaaaagtt atcttagatg gctggggatc tttatgattc tcggcttttt
    26941 gattcttgct agtccgattt acacacataa aaaggtagac ataccagatg aagtaagcac
    27001 gaacgcattt ttaaacacga aggaactgac gatggtttca aaagaaaaag gagcgaacca
    27061 gcaattagtt tttgaatttg cgatcgacga tgaagatcag agcttattaa aagaactagc
    27121 taatcttaat tatcgtgtag aagtaaagac agctaaaggg gattatcaag caataaaagc
    27181 caaagtgatc aaggtgtctg atgattattt tgtagtgaag ttagcacatg taccagaaga
    27241 atatattgct atgcgattaa ctattattcc tgaaaaaatt gatccaaagg tggatatgca
    27301 agaaccgcaa gatctgattt tttatattca cgaagataag gtcaaagatc acgtgaaaga
    27361 cgaagattat gaacaacatg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
    27421 agcagcacaa aaacaaattg aatccttcca agcaaccatt gatttgaatg aagaattagt
    27481 gaagaaacta aaagatcaat tgccttatca agtcgcagaa gatcaggaaa atactgaaaa
    27541 taagattaat agttaccagc aagaaattga aacatcgaaa acgcaaatga aagatcaaga
    27601 agagagcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa atatttagaa
    27661 gaataggtgg agtaaaagaa atgaagcgag cgaacttggt cggcaaagat actttcgctg
    27721 agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcaag
    27781 aagtgaatta tctaacacaa caatatctga gttgaagaat ggtagaaaga gtttagatac
    27841 cttaaaattc agatcatttg aagctctata tatagctgct aaaaaatatt ttacagctga
    27901 taataaataa aaaattaagg tgggttctat gtgaaaaaaa cgtatcaaaa gtttttatgg
    27961 attagctata tcgtcctatt tatcgttggg tttctcgtca gtgtgtccgt gatcgtcgga
    28021 acgctttttc ttttgttctc tgacgaagat ctggtactta aggggttaat gagtacgtta
    28081 tcgtgtgtca tgttacttta tttatgtata cagatcaacg aaattatgcc ccctcattgg
    28141 caagaaaaga agaaagaatt taaggaagaa atcgaaataa aatccaatga ctggattgaa
    28201 tacacatggc ttcctctgaa aagaaatagc aaacgaaagg aattttgggc attgcaatta
    28261 tggccaatag gaaaactttt tatttatatg gcttcactta tggttacttt aatcggttat
    28321 ttggtggtgg tctatggggt ggcctattac ttgttaagtg agccaaaaca gatcgcggcg
    28381 tcaaatattt tcacggcagt aatgacatta atgtgtgtca tgtgcttttc tatttttagt
    28441 tacttactcg tccaatttat caggcaaaat ttaacaatga gtaaactttt caaaagttta
    28501 ttggacttac ttatcttagg cagtatgtta acgactctaa cagatcggtt cgaacaatct
    28561 gaaaaaacgg tagaggggat aaagaacttc ttaacgacga ttgatcaatt tataaatgga
    28621 tatatgtatt tgtttgaagt aggctttatt ctcactttgt ttggttgtgt atttataaaa
    28681 ctcattaaaa gatcaatcga aaaagaaatg gttattgatc taattccgac gctgagcggc
    28741 tatgagtatt acgttgattt tggaaagtat gaggagttga tccatacgga tatcgttcac
    28801 acgagtatcc atgaagtaac cgttaagggc atcagatcta ctttaaaaga tggacaggag
    28861 acgaaaacaa tcgaccgaag aaaagaggtc tacaattttt ataaagttga agcaagtcta
    28921 cgatatggca ttgggaaatt gaggttagat gggagtaata ttcaacaaaa caaaccatat
    28981 gttttaggaa aagtggtgat ttagaacttc ctatgaaaga tgaataaata aggagaatct
    29041 tttgaaaatg attcttcttt acagaattgt gcttatagtt ggcacggagt taaaaatctt
    29101 aaagaaggag cagttattca aagtgaaaga acatcataat cagttactat gtattaaaac
    29161 gggtacgatg aacattcaat accgaacaga atatttagaa ttaacgaatc aaactgattt
    29221 tttgtcgatt cgagagcaac ataagcaaga aaaattaatg gctacttata gccaattgat
    29281 tgatgagtgg gaacaattca aggaatctaa aacataaaat tccatttaaa caccttattt
    29341 tttaaagagc ataaggaaac gtgtacccac tttcaaaata aagtgggtag ataggctgtg
    29401 cctatcacgt ttctgaaaaa aataaatgac cccaaaattt tcaatttatg tgtaggttta
    29461 tgggtagcaa aaatatgaat gtgggcagca aaaagttcat ttgtgggtag taaaattcaa
    29521 atgtgtgtag caatgtgtgt agctacacac attgctacac acgctataac atatagtgaa
    29581 aaggagatat ttatggaagt aaaaataaga gatattgatg aaaaaacgat tcaaagaatt
    29641 gaccgaagat gtattgaatt aagtgaacgc accgggacta aatggagccg aaatcgatac
    29701 ctaaaagttt tgattgaaaa tgattttgac catgaactta tggatcatag aaaagatcaa
    29761 tttgatcggt tgttagaaaa atttgtggat attcaaactt ataatacgaa aattttagaa
    29821 gaatatattg caacaaataa tcaattaatc gccttgttaa ttgaataata aaaatagtat
    29881 tgtgaattta taaaaggagg aaatcaattt tggcaaaagc aacaagaaaa catgtatatc
    29941 tttacgatat gcatatcgaa gtacttgatg aaataatttc tactcatgaa gatatatcta
    30001 attatagtga agcagtcaga catttgtgta tgatgtatga tccaaaaatc gaagaaaaaa
    30061 taaatccaat tcaaattaaa ttgaatgcaa tgggaaaaga aatttctata ttaaccgaaa
    30121 tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt ggtacaaatt
    30181 caactgtcta tcaagaagca aaagagcgtg tggaagggaa aataaaagcg aatcaaacga
    30241 aaaagtattc tcctaagatc aagaaaccaa taaatgaatc tgttcatgcc agagtagacc
    30301 cgtttgatcc aacaaagttt ttaaaaagat aaaaagtaga aggggaacat gatgaataaa
    30361 tcgcctgcag tcattctcac ttgtcaattt accttgccca ataaaaaatc tttttcaaca
    30421 tacattgatt atatgacaag agaaaaagcg ctggaagaaa aggaacgacg cacgccggac
    30481 gaagaacagg aactgcaacg aatcaagaat gctttagagg atttttatat tcctgaagga
    30541 gaaacttttt cggagagtaa gacagataaa gaattcagtg aaacgtcgaa tgaagctcaa
    30601 tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt taccaaatat
    30661 ctttcttata tgacccgtca ctatgcgtta gaacaaaaga aagaactgac gcaaagggaa
    30721 caaaaagaat accaacggtt gaaccaagca atgaaaaaat atcaaacaga gccacataaa
    30781 aaagcttcag gaaatcttcc tggagttttt tctatggctt cagacgaagt gaaaggaagt
    30841 gatctgaagg agatcaaaca aatcttccaa agagggcaac aaaaaggctc aatcatttat
    30901 caagatgttg tctctcacga caattcgtat cttgaaaaaa tgggcttgta cgatcccaaa
    30961 acagatacct tagatgaaga aggattaaaa gcagctggaa gaaaaatgat ggagacatta
    31021 ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca ccgtaacact
    31081 aagcatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa accttacacg
    31141 gaggttgaaa atggtgtgag ttatatcgta ccgaaaggta agagaagaca agcaacatta
    31201 gacgcaatga agaccaatta tgtacatgag ttagaacgtt tttcttatga aaaggatcga
    31261 atgattggta tggagaaacg aaatttattg acgcggaaaa gtgatttgcg taacacctta
    31321 acaaaagtgg taaaagagcc agcgcgttac gatcgacacg caatgaactt attaaatgaa
    31381 gtatataaaa ccctacctga caaacgttcg gaatggcatt atggggatga aaaacgaacc
    31441 aaactttcac catacacaag agagaaactc gatgagttaa ccaaatatgt gttaacaaaa
    31501 gaacctgaat atcaagaata tatacagatt tcaaaatcat tgaaagaaga agcaaaaagt
    31561 ctttatgggg aaagcaagag agaatcaaag gatgcagaaa aaaatgctct ctttgatttg
    31621 aagaagcgca caggaaatgc cattctaaca gaactgaaga aacaggatag tcaaataaaa
    31681 cctttaatag aaagtatgga tcagatgcaa tctatttaca atgaacgatc ctttcgaacg
    31741 cttaaacagt taccaacgaa tgaatacaaa tatagttacg aagaattcat gaagcgcagg
    31801 aaacaattcc aacaaagagt tgtcacgcaa cggagtttgc gtcaattaaa tcgaacgatt
    31861 aatgcccatg aagaaaaata ccacgcaagt aaagcctacg aagagcaaca acgaagaatt
    31921 caaatggaac aagaaagaca gtaaagacag taggaggaat agcaatgaaa caatatttag
    31981 atcaatggaa agttatagaa ggatcattaa gagaagaacg aatcgaacaa cttcccgatt
    32041 gtttagaaaa agagcatttg tttcagataa gagaaatgtt gagaaacgaa cagtttgatc
    32101 caaatcaatt tttagtcgtt gagtactcag ctactggtgt gtactgttgt aatcatgtga
    32161 agggtgaaaa atattttatc attcaagaat atgagggcaa gttagcgcct tattatacta
    32221 cttgggaaat gaatgaagag gggatcaata acttcccgtg taaaagcatt gaagagtcta
    32281 tttcactcac agaatgttag ttgttaagtg aataaaagaa gtaaaagagt cctgaacgta
    32341 attgtccagg actctttttt tcaatgaatt gttttccgcc aattcaatga gtggtgcgat
    32401 ttgcgactac attatatcaa aagaaagaga agggaggaag aagaaatggc ttattcaacg
    32461 attggagttg ttgttgaaaa aagcagagac aatctcgttt ttgtgacaga aattcaaacg
    32521 ggcagagctt ttgtcgtcac agacaaagcg gcaaaagcgt atcaaagtgg cgatatttta
    32581 acgttaaatc tgactacgaa gacgttcgtc gatgcggcag aagattatcc att
//
LOCUS       DACEKG010000028        32108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEKG010000028 DACEKG010000000
VERSION     DACEKG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.028"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(24..722)
                     /locus_tag="IW664_001217"
     CDS             complement(24..722)
                     /locus_tag="IW664_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0848592.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IW664_001218"
     CDS             complement(715..2067)
                     /locus_tag="IW664_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0848593.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IW664_001219"
     CDS             complement(2290..3690)
                     /locus_tag="IW664_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0848594.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IW664_001220"
     CDS             3851..4588
                     /locus_tag="IW664_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0848595.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IW664_001221"
     CDS             4713..5192
                     /locus_tag="IW664_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0848596.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IW664_001222"
     CDS             5189..5956
                     /locus_tag="IW664_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848597.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IW664_001223"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IW664_001223"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0848598.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IW664_001224"
     CDS             complement(6859..7461)
                     /locus_tag="IW664_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0848599.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IW664_001225"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IW664_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IW664_001226"
     CDS             8142..8726
                     /locus_tag="IW664_001226"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0848600.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IW664_001227"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IW664_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0848601.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IW664_001228"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IW664_001228"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0848602.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IW664_001229"
     CDS             10627..10806
                     /locus_tag="IW664_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848603.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IW664_001230"
     CDS             10828..11853
                     /locus_tag="IW664_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0848604.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IW664_001231"
     CDS             12028..13272
                     /locus_tag="IW664_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0848605.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IW664_001232"
     CDS             13425..14372
                     /locus_tag="IW664_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0848606.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IW664_001233"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IW664_001233"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0848607.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="IW664_001234"
     CDS             complement(15274..15516)
                     /locus_tag="IW664_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848608.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="IW664_001235"
     tRNA            15637..15709
                     /locus_tag="IW664_001235"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="IW664_001236"
     CDS             complement(15828..16466)
                     /locus_tag="IW664_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0848609.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="IW664_001237"
     CDS             complement(16589..17221)
                     /locus_tag="IW664_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0848610.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="IW664_001238"
     CDS             17572..17871
                     /locus_tag="IW664_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0848611.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18366..20531
                     /gene="acm"
                     /locus_tag="IW664_001239"
     CDS             18366..20531
                     /gene="acm"
                     /locus_tag="IW664_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0848612.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20803..21288)
                     /locus_tag="IW664_001240"
     CDS             complement(20803..21288)
                     /locus_tag="IW664_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0848613.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21768..22409
                     /locus_tag="IW664_001241"
     CDS             21768..22409
                     /locus_tag="IW664_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0848614.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22428..23063
                     /locus_tag="IW664_001242"
     CDS             22428..23063
                     /locus_tag="IW664_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848615.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23081..23896
                     /locus_tag="IW664_001243"
     CDS             23081..23896
                     /locus_tag="IW664_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0848616.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24215..25651
                     /locus_tag="IW664_001244"
     CDS             24215..25651
                     /locus_tag="IW664_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0848617.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25840..28287
                     /locus_tag="IW664_001245"
     CDS             25840..28287
                     /locus_tag="IW664_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0848618.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28517..29584
                     /gene="rlmN"
                     /locus_tag="IW664_001246"
     CDS             28517..29584
                     /gene="rlmN"
                     /locus_tag="IW664_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0848619.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29777..30880)
                     /locus_tag="IW664_001247"
     CDS             complement(29777..30880)
                     /locus_tag="IW664_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0848620.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31433..31981
                     /gene="aac(6')"
                     /locus_tag="IW664_001248"
     CDS             31433..31981
                     /gene="aac(6')"
                     /locus_tag="IW664_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0848621.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        11023 a         5470 c         6523 g         9092 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tatagattga aaaaatgaaa gcaataagta aaataaaatg aatatatgcg aaaaaataat
    18181 ttacattagg aaaaaccttg taaaaacagt tttttggcat atttattttt tataaatatt
    18241 tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa
    18301 gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag
    18361 aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa
    18421 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
    18481 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
    18541 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
    18601 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
    18661 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
    18721 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
    18781 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
    18841 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
    18901 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
    18961 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
    19021 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
    19081 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
    19141 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
    19201 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
    19261 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
    19321 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
    19381 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
    19441 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
    19501 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
    19561 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
    19621 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
    19681 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
    19741 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
    19801 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
    19861 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
    19921 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
    19981 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
    20041 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
    20101 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
    20161 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
    20221 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
    20281 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
    20341 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
    20401 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
    20461 ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata
    20521 aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa
    20581 attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc
    20641 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
    20701 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
    20761 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
    20821 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
    20881 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
    20941 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
    21001 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
    21061 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
    21121 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
    21181 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
    21241 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
    21301 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
    21361 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
    21421 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
    21481 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
    21541 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
    21601 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
    21661 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
    21721 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
    21781 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
    21841 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
    21901 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
    21961 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
    22021 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
    22081 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
    22141 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
    22201 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
    22261 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
    22321 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag
    22381 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
    22441 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
    22501 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
    22561 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
    22621 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
    22681 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
    22741 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
    22801 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
    22861 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
    22921 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
    22981 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
    23041 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
    23101 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
    23161 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
    23221 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
    23281 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
    23341 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
    23401 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
    23461 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
    23521 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
    23581 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
    23641 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
    23701 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
    23761 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
    23821 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
    23881 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
    23941 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
    24001 acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa
    24061 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
    24121 caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag
    24181 gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt
    24241 tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca
    24301 agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt
    24361 taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc
    24421 tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt
    24481 tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga
    24541 cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca
    24601 agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag atttcactca
    24661 ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa
    24721 tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac
    24781 agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat
    24841 caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc
    24901 aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct
    24961 gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag
    25021 gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga
    25081 atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa
    25141 tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga
    25201 agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa
    25261 ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg
    25321 ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga
    25381 agataaacaa atccatgacg actatcgaat caattactta caaagccacg tcaaagccat
    25441 caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga
    25501 tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga
    25561 cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa
    25621 actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc
    25681 tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat
    25741 aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt
    25801 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
    25861 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
    25921 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
    25981 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
    26041 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
    26101 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
    26161 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
    26221 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
    26281 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
    26341 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
    26401 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
    26461 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
    26521 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa
    26581 tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa
    26641 ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat
    26701 atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca
    26761 gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa
    26821 gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca
    26881 actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa
    26941 actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc
    27001 acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca
    27061 gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt
    27121 aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa
    27181 atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat
    27241 gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt
    27301 tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt
    27361 gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg
    27421 tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa
    27481 gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga
    27541 gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg
    27601 aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg
    27661 gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg
    27721 gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc
    27781 aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg
    27841 atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca
    27901 atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa
    27961 cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg
    28021 acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa
    28081 gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt
    28141 cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt
    28201 ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa
    28261 acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa
    28321 ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc
    28381 taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta
    28441 tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa
    28501 aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat
    28561 caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt
    28621 gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga
    28681 aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc
    28741 ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact
    28801 gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg
    28861 ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat
    28921 tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt
    28981 ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga
    29041 ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt
    29101 ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa
    29161 tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa
    29221 ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa
    29281 ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc
    29341 gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc
    29401 atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag
    29461 attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac
    29521 agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa
    29581 ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg
    29641 aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga
    29701 caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg
    29761 gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat
    29821 gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca
    29881 catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga
    29941 cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga
    30001 ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga
    30061 cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg
    30121 agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa
    30181 caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt
    30241 tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc
    30301 ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt
    30361 tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg
    30421 ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc
    30481 cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg
    30541 gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac
    30601 ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact
    30661 gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt
    30721 cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa
    30781 tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg
    30841 ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc
    30901 gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag
    30961 aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca
    31021 ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg
    31081 ttcctcttag tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa
    31141 aattaagatt attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa
    31201 agcaaaagat aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt
    31261 tcctgatctt tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa
    31321 gataaataat gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat
    31381 ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat
    31441 cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact
    31501 gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc
    31561 agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat
    31621 ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg
    31681 aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg
    31741 aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac
    31801 ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc
    31861 gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg
    31921 ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata
    31981 ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa
    32041 ttacttcttg aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac
    32101 cgtgtacc
//
LOCUS       DACEKG010000029        31662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEKG010000029 DACEKG010000000
VERSION     DACEKG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.029"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..345
                     /locus_tag="IW664_001249"
     CDS             <1..345
                     /locus_tag="IW664_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848622.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDNNDQYLPKK"
     gene            426..1046
                     /locus_tag="IW664_001250"
     CDS             426..1046
                     /locus_tag="IW664_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848623.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1376..2602)
                     /locus_tag="IW664_001251"
     CDS             complement(1376..2602)
                     /locus_tag="IW664_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0848624.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2674..2832)
                     /locus_tag="IW664_001252"
     CDS             complement(2674..2832)
                     /locus_tag="IW664_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848625.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3520..4260
                     /locus_tag="IW664_001253"
     CDS             3520..4260
                     /locus_tag="IW664_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848626.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4352..5539)
                     /locus_tag="IW664_001254"
     CDS             complement(4352..5539)
                     /locus_tag="IW664_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0848627.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            5861..6703
                     /locus_tag="IW664_001255"
     CDS             5861..6703
                     /locus_tag="IW664_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0848628.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6700..7650
                     /locus_tag="IW664_001256"
     CDS             6700..7650
                     /locus_tag="IW664_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848629.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            7665..7898
                     /locus_tag="IW664_001257"
     CDS             7665..7898
                     /locus_tag="IW664_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0848630.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            8229..8813
                     /locus_tag="IW664_001258"
     CDS             8229..8813
                     /locus_tag="IW664_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0848631.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            8944..9612
                     /locus_tag="IW664_001259"
     CDS             8944..9612
                     /locus_tag="IW664_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0848632.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            9614..11038
                     /locus_tag="IW664_001260"
     CDS             9614..11038
                     /locus_tag="IW664_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0848633.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            11056..11625
                     /locus_tag="IW664_001261"
     CDS             11056..11625
                     /locus_tag="IW664_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0848634.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            11735..13489
                     /locus_tag="IW664_001262"
     CDS             11735..13489
                     /locus_tag="IW664_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848635.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            13443..15215
                     /locus_tag="IW664_001263"
     CDS             13443..15215
                     /locus_tag="IW664_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848636.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            15738..16007
                     /gene="rpsN"
                     /locus_tag="IW664_001264"
     CDS             15738..16007
                     /gene="rpsN"
                     /locus_tag="IW664_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0848637.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            16022..16201
                     /gene="rpmF"
                     /locus_tag="IW664_001265"
     CDS             16022..16201
                     /gene="rpmF"
                     /locus_tag="IW664_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0848638.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            16233..16610
                     /locus_tag="IW664_001266"
     CDS             16233..16610
                     /locus_tag="IW664_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848639.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            16631..16780
                     /gene="rpmG"
                     /locus_tag="IW664_001267"
     CDS             16631..16780
                     /gene="rpmG"
                     /locus_tag="IW664_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0848640.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            16804..17787
                     /locus_tag="IW664_001268"
     CDS             16804..17787
                     /locus_tag="IW664_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0848641.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            17817..17939
                     /locus_tag="IW664_001269"
     CDS             17817..17939
                     /locus_tag="IW664_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0848642.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            17957..19480
                     /locus_tag="IW664_001270"
     CDS             17957..19480
                     /locus_tag="IW664_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0848643.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            19504..19743
                     /locus_tag="IW664_001271"
     CDS             19504..19743
                     /locus_tag="IW664_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848644.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(19936..20397)
                     /locus_tag="IW664_001272"
     CDS             complement(19936..20397)
                     /locus_tag="IW664_001272"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0848645.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(20592..20810)
                     /locus_tag="IW664_001273"
     CDS             complement(20592..20810)
                     /locus_tag="IW664_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848646.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            20928..22544
                     /locus_tag="IW664_001274"
     CDS             20928..22544
                     /locus_tag="IW664_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0848647.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(22785..24125)
                     /locus_tag="IW664_001275"
                     /pseudo
     CDS             complement(22785..24125)
                     /locus_tag="IW664_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(24401..24556)
                     /locus_tag="IW664_001276"
     CDS             complement(24401..24556)
                     /locus_tag="IW664_001276"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0848648.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(24748..24942)
                     /locus_tag="IW664_001277"
     CDS             complement(24748..24942)
                     /locus_tag="IW664_001277"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0848649.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(24852..25184)
                     /locus_tag="IW664_001278"
     CDS             complement(24852..25184)
                     /locus_tag="IW664_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0848650.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(25698..25889)
                     /locus_tag="IW664_001279"
     CDS             complement(25698..25889)
                     /locus_tag="IW664_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848651.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(25983..26369)
                     /locus_tag="IW664_001280"
     CDS             complement(25983..26369)
                     /locus_tag="IW664_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0848652.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            27063..28268
                     /locus_tag="IW664_001281"
     CDS             27063..28268
                     /locus_tag="IW664_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0848653.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            28285..28719
                     /locus_tag="IW664_001282"
     CDS             28285..28719
                     /locus_tag="IW664_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848654.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            29336..>31662
                     /gene="esp"
                     /locus_tag="IW664_001283"
     CDS             29336..>31662
                     /gene="esp"
                     /locus_tag="IW664_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0848655.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT        10867 a         4615 c         5823 g        10357 t
ORIGIN      
        1 tcaaatataa aaactagtac gaaattaatt tgtaaattat ttaagcttga taaaataagc
       61 aatatcgatg atgtaaagtc caagatatta acaagaaaag taagcgaacc agtaaagcta
      121 acggcacaag ataaaaaaaa tggaattgtt ctttatggtg atgatgcaaa gtataactat
      181 aattgggata cctcctcaga agctgaggta ttaagtattc ctactaccga tacttcaggg
      241 gaaaaagtca cctttaaaat aaattttata acggcaattt ctaagctgga gtatgggaag
      301 caaaaaaaga gagataacaa tgatcaatat ctacctaaaa aataacgaat taatgatgca
      361 tatgttaaag ttgttttttt gaattaaaca actcaattta ttcagttata tgttcatcgc
      421 agcaagtgaa cattaatcga gttataaaat taataggagc taaaaatata aataaagttc
      481 gatcagaatt tatttttcca gaagattttt ttaactggtt tttttatcaa ttcagtgaat
      541 gcaagcgact cttaggtgag aattattact taaaaggaat cggcggattt atggggaatg
      601 tggctgatga acaaaatatt ataaaaggaa tatctgaaca aacatcagag cttacagtaa
      661 ctaaagcatt tataagtaat ggtgaaagtt tgaaaaatgt aactgcgcga attagaaaag
      721 aaaatgaagt agaaattgtt tttggaattg accatgaatg taattcagtg atatatgtaa
      781 cacaatcagc aaatttaatt ctttttgaga atggttgcaa ggacgatttt atggtggtct
      841 atttgtacga tatactaatt ccaaaacttc gttccttata tatgtcttct gaaagtattt
      901 ttgtgagtga acaaaaggga tctttctcga aaaaaatagg tattgaagta attgaatcga
      961 ttatgtgtca taatcatatt acgctagaag aattagaaat tttgataggg caaaaaaata
     1021 ataacacgaa aatacaaatt tcttagaaat cattttttat tgagatatct agataaatga
     1081 tttattcttc aatcaaccaa ctcgccaagc tgaggtaatg ttttgaggta atgataacat
     1141 acaacttttt ataccctagt aaaactctat aaacaaagac ttattaattt cgcatgaaat
     1201 acattcctgt aaaatgcaat aaaatagagt tttgtttaac caggtctcaa aaaggctcgt
     1261 aaagcatctc aattctctaa acgttaatat tatttatata tcaatgtttc aaggcacttt
     1321 tcaattgttt gaaaagtgtc tttttcttgt ttaagaacac atttaagaac acatactaat
     1381 cacatttatt caatcctctc tgaaaggctt caaaaacagt tttatctact acatctggtg
     1441 tatttacgta tattctcgtt gtagaaatat ctgagtgtgt taaagcacta gatatttgat
     1501 tcatatttgc tccgccttgt ctagctatag ttgcataggt atgacgaagc ttatgtggag
     1561 ataaatgagc caattcagga tgtcgtcttt taattgaatt gattcgatag tttaggtaat
     1621 cagcgtgaac agggcaattt acttctccgg atggtttagt ataggtaaag agatactgat
     1681 ctggtgtttg attaattttt aatctcaaca attgctcagc ttgcactgat ttccatttgc
     1741 ttagtaatgc cattacaatt tcgggtactt gaattacggt atcctttcga ccttttgtaa
     1801 atttcttttt ttgatattta tccaaagcat gtttaaggcg aaccgtttgt ttctcaaagt
     1861 cgatatattt ccattgcaaa gcatacgtct cactttttct atcaccaaga tatagggtaa
     1921 gcatgaaaag agtatagtca tgaagagtaa gcaagtgatt attcaagtca gtgtttactg
     1981 cttctatcca agcaataagc tcttctgacg ataatgcttg attaccttcc cgtatccttc
     2041 tttcttctaa agcaattttt ttaggtgcag tgatacattg aataattttg gtagttcggt
     2101 taaattcaat ataatttagt atttctgcaa tatcaaacat agaacggaca tatcctttga
     2161 taatttttat atttgcgtat tctttcgatt tttttgttaa ttcatctgat attatctcag
     2221 tatttgaatt tagatagttc attgcatact caccaaacat tggaaggatg tgcaatcgga
     2281 atatatcttt agtattacta attgttacgt aacttggagc tctttttgat ctagtagtct
     2341 gacctagttc atatctagga agccattttg attcatagaa ttccttaaat tttatttttc
     2401 cttttaattc tagtgaattt gcattctcct ctcttaaaac tgtttcaatt ttccttttca
     2461 ttgcattttc ggctttaata gccaattgcc tagttggaaa tactttttga tacttttctg
     2521 atgtaattcc agtgatttct ctgaattttt ttggatacct cagattagtt ttatatttcc
     2581 catttgaaag ttttgtaggc aaaattcctc actcccttaa aaagtaaggc agtatatttc
     2641 gctattctta gtatatgcgg cgagaagaaa ttttcaaatt gttttaaatg tattcataaa
     2701 atctttgata tcctctatgt caaagcgaac tataccgtca attctgatta ctggaaaatt
     2761 atatatacga atccacttat ctaaagtatt attatcaatt cctagaaact cacaggtttg
     2821 ttttttattc aagtagactc tcaattttat aaaatcatct cctaaaaaag attagaattt
     2881 ttgaattgct ttgggtaagc acataggaat ttcacctctc cccagtttac ggcgagcctt
     2941 acggaagtat cattatttgc tgctattgtc atggcatttt gttttcccag aacaaaaaga
     3001 aatctctaag tatttgtcgc taatcaggtc atggcgtact tgagtttcta gctcgaatag
     3061 cagggatcgt tattcaaaaa ttgtatttaa ttgtcaatga tcaaaaatag gttttttcct
     3121 attcactatt aataacgata aacatgtata aaaagacaca ctacgaaaaa agttaaatgg
     3181 agtttactga ttttcatatt tgtatatgga acagacatag tcttgaataa tttttataaa
     3241 tgttttgatg cttatcaact tattcaaccc gctatccact ttaagtataa tttttccata
     3301 cgccaataaa gtagttttag gagcatgttc tcatttgaga acgctccttt tttatatatt
     3361 ttgaaagcta cttactatac gcgtagtttc aaatagtttt gtcattctaa tcttcagcaa
     3421 cagaaaaaaa tctaaattat aaaatttagg gttgactaaa atataatttt tgtataccat
     3481 atgatcgtga taatcattat catttagaag gagaaagtta tgttagaagt gaaaaagctt
     3541 aatgtttgtt ataacgattt tttagcattg aacgagattt ctttaaaaat acaagaaggt
     3601 gctttaacag gtattattgg tccaaatggt gctggaaaat ctacattgct taaagctatg
     3661 ttaaatatta tccctcatca gggagaggtc tccattaata atgaagatat caataaaaaa
     3721 ctggtgaaga ttgcatatgt agagcaaaaa gctgatattg attttacttt tccaataaaa
     3781 gtaaaagaat gtgtatctat gggaacatat gccggaatga aagtttttca aagaattaaa
     3841 aatgcagaat ggcaaagagt ttcaaaggct ttggaaaagg tcgatatggg aaaatattct
     3901 aatcatcaaa taggagaatt atctggagga caatttcaac gtgtactttt agcaagatgt
     3961 cttgctcaaa atgcggattt catattttta gatgaaccat ttgtagggat agacttagtt
     4021 agtgaacgaa taattatgga tacgttaaaa gaattgaaac atcagggaaa aacagtgcta
     4081 attgtgcatc atgatttaag caaggtaaag aagtattttg ataatattat tcttttgaat
     4141 cgaaatctaa ttgctcacgg aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa
     4201 gcctatggag atactatatt tattggagag actaactata tagagtcaag taaaaattag
     4261 ccaaatcaaa aaatatttta taacagataa ttccgaaaaa agggtacaac aaataagctt
     4321 cttaccatta gtttttttga atgttcaata gctaatcaga aacaattata taaggctcgt
     4381 ttttagtcaa aatagcatga atcacgtaac aaagcttacg agaaaccgca cctaaggcag
     4441 ttagatgatg ttttccttcg gaaacttttt tctggtaaaa cgcactaaaa acaggatcac
     4501 attgagaagc tactaaagcc gccgaaaaca aggcttttcg caaatatgga gaccctcgtt
     4561 tactcataac attatgcgtt gcctcatatt gccctgattg tgtcacggaa gcgtctatcc
     4621 cagcgtaagc aacgagtttt ttcgggttag aaaatttttg aatatctccg atctcaccaa
     4681 gaatagtcgc accgtttata ggaccaattc caggaattgt gagaataaca gaatttaaag
     4741 tgtccattat gtgcttgatt tctctttccg tttcctttat ttggctttca atgaatttta
     4801 attgttcaat cattgaacgt agttggaagg tgaacgcttc ttgagcaaat tttactccaa
     4861 aggagttaga agctgcttgc ttcaattggt ccgctttttc tgccttgatt cggttcctgc
     4921 ttaactcagc taagtattgg gccaaagttt ctgaggaaat gttttctaag tcgatcgggg
     4981 cactaaacgc cagcaataac tctttagaag cttttccgaa aacgccctgc ttggtaaaaa
     5041 tcgtcgcgta ttctggaaag acttgatcca atacggctat aatttttctt ttgaagtcgc
     5101 tcgctgtacc tactaaataa gttcggtaac gagaaagttg tttcaaagaa aaaacatttt
     5161 catcggataa gaccgtttcg acaaatgagc catagcgcat taagtcagca atcaaaacag
     5221 aatcaataat atcgtttttt cgtttccgta tttctgtacc tttacgccat ccgtctgttt
     5281 gaaggggatt cacaacgtga atcaaataat ctgattcgtg aaggtatgaa aagatggaca
     5341 gccaatagtg ccccgtggct tccatcccta taacaaagtc actgggtgct ccggaatagt
     5401 cgaccattcg ctcaattaat gactctgctc cttcagtggt gttaggaaat gaaaaacctt
     5461 ttagtaatac ctttccttcc tcgttcatga tagaagcaac gtgagtgcgt ttaccgatat
     5521 caatgcctaa aaagtacatg tatatctgac ctttcaaaaa aagaattttg gattgaaatc
     5581 cactcaactg aaaagcgtca ctaccttgta cgagatacgg agtaaagtaa ggactttcaa
     5641 catctaactc atacgcaatc cacttatcag agagaggagc cagtctctcg agtgcgggtt
     5701 caatggccca aggggggaac ggcttagcct ctcaatccaa taaatatatt atctcaaaag
     5761 aaatgagtta ataccaaatt aaaataacga tggttcatag ggttatccaa tcaaaaccgg
     5821 aaggttaaaa ccctaactct aatatacaag ggggaataat atgcttcaaa attttattaa
     5881 tggtctgtat gactttcact ttttacagaa tgctctaatt acatctgtag ttatagggat
     5941 tgtttctgga acagtaggtt gttttattat tttaagagga atgtctctta tgggtgatgc
     6001 tatttctcat gctgttttgc caggagtagc aatttcttat attttaggta ttaatttttt
     6061 tattggtgcg attgtatttg gtctattagc ttctatcatt attactttta taaaaagcaa
     6121 tagtgtaata aaaggagata cagcaattgg tattactttc agttcttttt tagcattagg
     6181 agttatcctt ataggagttg caaatagctc aacggattta tttcatatct tatttggaaa
     6241 tattttagct gtacaagata ttgataagtg gattacaatt ggcgtttctg ttttagtgtt
     6301 ggttacagtt gttctatttt ttaaagaatt attaatcact tcttttgatc cattaatggc
     6361 aaaagcaatg gggatgaatg tcagttttta tcattatcta ttaatgattc tattaacttt
     6421 agtagcagta acagctatgc aaagtgtggg aactatatta attgttgcaa tgttaattac
     6481 accagctgct acagcatatt tatatgcaaa cagcttaaaa actatgatat ttatttcggc
     6541 gacagtcgga gcgtgctcat cattgctagg tttgttcatt ggttatagtt ttaacatagc
     6601 agcaggatca agtattgttt taacagcggc attcttattt gtaattagtt ttctaatctc
     6661 accaaaacaa aaatttttaa gaaagaagga gagaaagtta tgatgagaaa atggaaagca
     6721 gtactgggaa gtctggggat tttgattgct ctttttatat tcggggcatg ttcaacaaat
     6781 agtaaagaca aagatacagt ggcttcgaac gaaaaattaa aggtagtagt tactaattcg
     6841 attttagcag atattactga aaatatagca aaagataaaa ttgatttaca cagtatcgta
     6901 cctattggga aagatcccca cgaatatgaa cctttgcctg aagatgttca aaaaacttca
     6961 aaagcagatt tgatttttta taacggtgtt aacttggaaa ctggaggaaa tgcttggttt
     7021 acaaaattag taaaaaatgc gaacaaagag gaaaacaaag actattttgc agcaagtgat
     7081 ggcatagatg ttatttactt agagggtcag agtgagaaag ggaaggaaga tccccatgct
     7141 tggttaaatt tagaaaacgg tattatttac gctaaaaata ttgaaaaaca gttagcggaa
     7201 aaagatcctg ataataaaaa attctataaa gaaaatctag ataagtatat tgaaaaattg
     7261 gattctctag acaaagaagc taaatctaaa tttgcttcaa ttccgaatga taaaaaaatg
     7321 attgttacaa gtgaaggatg ctttaaatat ttctcgaaag cgtataatgt gccttctgct
     7381 tacatttggg aaatcaacac tgaagaagaa ggaacaccag atcaaataaa acacttagtt
     7441 gaaaaattac gcacaacaaa agttccctcc ttattcgtag aaagcagtgt ggacgataga
     7501 ccaatgaaaa cagtatcaaa agataccaat attcctatct attcaacgat ttttactgat
     7561 tcaattgcag aaaaaggaca agatggtgat agttactatg cgatgatgaa atggaacctg
     7621 gataaaattg ctgaaggcct ttcgaaataa tttggagggt aggtatggta caaattaaaa
     7681 cgactactat actaatttat ttactagcaa ttcaggaatt atcaaaaaga aaaaagaaat
     7741 taaaaaataa tgatttagca aaagttctaa gtgttagtcc agcatctgta agtgagatgc
     7801 tggctaaact tagatatgag gactatttag atataggatt tcaattaact gataaaggga
     7861 aaaaatttat tgacgattac aaaaaaggtt ttaagtaaaa gactttattt ttagggagga
     7921 taagaaaatg gctattggag tgattatgat aggacttacc ctaatagctg gatataattt
     7981 ttttaaatct ttctcggatt attttagaaa aggtgaatga tttagatgag tctattaatt
     8041 cttgtaggat tgctttttgt atcagttatt ttgctctttc tcattagttt agctctaagg
     8101 aatgagttca gtaatagacg ttaacgattg aaacttcaag tcctgtgtaa gataatatag
     8161 ttaattcatc ttattaggag gaagtaccca tagaaaccag agattatatt acaactcacc
     8221 aaaaattaat agaaagtgga ctaagccatt ttgaaaaaga aggatttcag caaactaaga
     8281 taaggcaact gtgcttgacc gcaggcgtaa ctacgggtgc tttttataaa cattttactt
     8341 ctaaggaagc gttatttgaa gaaattattc agccgcatat taatgcgctt attaatatgt
     8401 ataatgaaga gtttaccaaa tacctcttaa acttagaaaa tatagatata gagaaaagtt
     8461 ggagagagaa cttcaatgaa ttagatccat ttattaatta tatttttgag aataaacgta
     8521 tattcgattt ggttttattc caatcagctg gaaccaaata tcaagatatt gttgaaaaaa
     8581 ttacatcatt tgtgacaaaa aagacagtct gttgtctaga taagttaaag actatcggaa
     8641 tgatcaatca aaatttagtc tttaatgaag aagaaatcca cttctacatt tactcattct
     8701 acgctacatt ctatgatata ttgaagcata gctattcaaa agaaaagact atgctattaa
     8761 ccagaagatt gtatcaattt tctgttcctg ggtggattga attactgagt taattaaaaa
     8821 aaagcttgca ttgagaattg ttctcagtta tgctttttga taagatcgca ctgttatctg
     8881 aataacggtg catttttgtc agcttaagat aacagtgcga tcttattttt tggaggtgaa
     8941 agagtgtcaa aaatcaatcc gttaaccttg attactttaa atattttttt tccggtcatt
     9001 atttttttag gaaagggaaa tatatttcaa gtggggtgtt tcattattgc tacagtttta
     9061 gtattgatgt ttcgtaaaat aaagacatgt atttatttca tcttgattta ttcagttctg
     9121 tttttagtaa tgaaaattat tgatgctatc aagatagagt tttttactat attttttggg
     9181 acatccttat atattgtatt tcgaatgttg ccggtcgtaa tgatctcatg gttgttggtt
     9241 agtgtctata ctagcaatga attattatcc tctttagaaa aaatacattt accgaaaaaa
     9301 ataatgttaa gtattacagt agtgatcaga ttctttccga cttatcgttc tgaaataaga
     9361 atgattaaag aatctttaaa aatgagaaac attcgcttgt ctttttttca gcctctgcaa
     9421 tatttggaat ttctgttagt tcccgttctt atacgtgcaa ctatgattgc tgaagaaatg
     9481 accgctaatg caataacaaa ggggatagaa tctcctgtga aaagaagttc ttactataaa
     9541 acaaacatga gcttattaga ctatatcatc ctgttttccg ttactatttt tttcttaagt
     9601 attttgattt gaggtgaaag aattgtttga atttaacaat gtttcttttt catataagga
     9661 tgttaaggaa caagcgcttg ttgaccttaa tttttcaata aaaaaaggca gtcttacatt
     9721 gattactggt tcttctggga gtgggaaatc cacactatta aagttaatga atggacttat
     9781 tccaagactt tatgacggaa aattggttgg ggaaatttta tttgaaaata actcgctaca
     9841 atccttaaca caagaaagta tatcaaaaaa tattggctac gtaagtcaaa atcctagagg
     9901 acaattcttt acaacaaata caacgagtga gttagtattt tcgatggaaa actttggatt
     9961 tacaaaagag catatggata aaagaatcaa tgaattagct gaattattag atctttcaaa
    10021 tatattagat aaaaacattt tttcgctatc aagtggtgaa cggcagaaaa tttctattgg
    10081 atgtagttta tcgctaaact ctaaattaat tatattggat gaaccgtctt caaatttgga
    10141 cttcaaagcg actaagaaac ttgctgactt actttgtgat ttaaaaaata aaggatttac
    10201 tattgttata gctgaacatc gtttttacta tttgaaagaa ttaattgatc aagttctatt
    10261 tgttaaaaat agaactgtaa aaactacaac tattcagttg ttaaaaactg aaaacaataa
    10321 cgcattaagg aaatttgaca tttttaatag ttcgattgaa gttccctata tcgaaaaggt
    10381 aagtgcagat gtattaaaag ttgaagaaat tacttatgaa aatattctaa aagacataaa
    10441 ttttgatgtc aaagttggag atgttattgg tttggtaggg aaaaacggtg tggggaaaac
    10501 aaccttacta agaatactca tgaagattat tcgccctacc aaaggaaaga taatcgaaaa
    10561 taaaaaaaat ttgtcatcgc cgtttttagt aatgcaagaa atggattacc aatttttcac
    10621 agaatcagtg aggagcgagt tagccctggg gaatgaaata gttagtaaag atcagcaaga
    10681 aaaaatttta aaaaaaatgg aactttataa aatgaaagat caattaccgt ttgatttatc
    10741 cggtggagaa aaacaaagat tattggtttc tatagcttca ttgtcaaaaa ctaatttatt
    10801 tctatttgat gaacctacaa gtgggttgga ttatatcaat atggaaagga ttgctgacct
    10861 tattaaagac ttaaaagaaa agggtgcagt agttatagcc actcatgatc cggaatttct
    10921 ttataaaaca tgcaatagaa ttatttattt aaaaaatggt ggaataaaaa aagatataaa
    10981 acttagagta gaagattcaa ctttgataga acagattttt aatgacataa cgaaatagaa
    11041 ttgaggagaa tatatatgaa aacaaaagat tatattttta ttggtttagc tacagttatt
    11101 tccctagtaa tttacttttt ttcaataatg gttagtagta ttggtggggc gtttggtcac
    11161 agcatatccc cagggatttt tggattatta tctggaatca tatttgttta tattagctat
    11221 aactacccta gaaaaggaat ttttactatt tacacaattg ttttattgtt attttttacg
    11281 ttaatgggcg gggcgtactt accttggttt atcagttcga ttagtatggc gatcttagct
    11341 gatattattc tatctgtatt tggatatgat cgagcaattc ctcaagttgt gtcttgggtt
    11401 ctaatgcaac taggcagtgc tgccggacag tggattccca tatggttctt tacagatcgt
    11461 tttcgtcaag actggataga tagaggacaa tctgctgcaa caatggatgc aatgatccac
    11521 tacgcggtgg gtatatgggg aattatttca gtgttagttg tagcaagttt atctatgatt
    11581 ggtgtattga ttggaagaaa agtcttaaaa aaatacaaaa aatagaacgt agtttacctt
    11641 ctatctgttt gtacatctct aatttacgct attacggaaa gcattaacag ttgtaaacga
    11701 ataagagaag aaattgaatg cgggggagga aaaaatgaaa gtatacaaaa aattatttag
    11761 ttatgtccct aaaatgaaat actacggatg gttagccact ttattttctg atttttctgt
    11821 cgtattaaca gtctatggat attacagtat ttacaaattt cttcagtctc taattattgt
    11881 aggagatgaa tatttagcta agagttatgc ggttcaaact gttgcttggt taactttagg
    11941 agctttattt tatatttttt ctggcttatt ttctcatatc cttgggtttc gcttagagac
    12001 taatttgaga aaaaagggaa ttagcggttt aacagaagca agttttcgtt tttttgattt
    12061 aaattcttcg ggatatattc gaaagacaat tgatgacaac gcggctaaaa cacatatggc
    12121 aattgctcac ttgattccag atagtgctca agccatagta acacctgttt tatcagttgc
    12181 cttaggcttt ttcattaact ttaaaattgg attggttcta atcgccatgt tagtaattgg
    12241 tgtattcttg ttaaagaaaa tgacagagaa tacaaatttt attcaaaaat atcaagaatc
    12301 gttagatgtg ttaagctctg aaacggtaga atatatccgc ggaattcaag ttataaagat
    12361 atttggtgct aaagtaacat catttaaagc cttgaataaa gcaattaacg attatgctaa
    12421 gtttgcatat aagtattctt tgagtgggaa aacacctttt gttctttttc aatggctatt
    12481 ttttggcata gtttgcattt tgatagttcc tataaccttt tttataactt ctcttaccac
    12541 tcccaagatc ttagctgttg acttgttgat gttgtttttt ttaagtggaa ttatttttgt
    12601 ttctttcatg agaataatgt atgtaagtat gcacttgttt gaagcaagat atgcagtcga
    12661 aacactagac tccctttatt tagaaatgaa gcaagaccaa ctaaaatatg ggaacgagac
    12721 acagattaaa aattgtgaca ttgcatttga aaatgtcagc ttttcataca aagaagagtg
    12781 tgttttagaa aatttcaact tagttttaga acaaaaaaga atttatgcat tagtaggaaa
    12841 atcaggaagc ggaaagagta cgatagctaa actaatttca ggtttctaca atgtggataa
    12901 gggacagatt aaaattggtg gtaagccttt aaatcactat tcaaaagaca caattattaa
    12961 cacaattgct tttgtctttc aagatacgaa actattcaat cagagtattt acgataacgt
    13021 ttctatagca aatcctcaag cgccagcaag taaagtattt gacgcgctaa gattagccgg
    13081 ttgcgaatcg attttagaaa aatttcccga aagagagcat accttaattg gtagtaaagg
    13141 agtttatctg tcaggaggag aaaaacaacg tatagctatt gccagagcaa ttttaaaaga
    13201 tgcaaagatt gttattatgg atgaagccag tgcatctatt gatccagata atgagtatga
    13261 actacaaaag gcttttcaat atttgatgaa agaaaaaaca gttattatga tagctcatcg
    13321 attatccagc attaaatctg tagatgaaat tcttgtcttg tcagaaggaa aaatagtaga
    13381 acgtggaaat cataatgaat taatggctaa acatcgagtg tataaacaat tggtcactaa
    13441 ttatgagaat gcaaatgatt ggaggcttga caatgaaaaa tttttataaa aaaaaatttg
    13501 ctttaactga tcagggcgca gaagctttaa ctaaagcttc tatttctagt ttttttgtat
    13561 actgcattaa tatggtacca gcattcatta taatgatgct gatagacgaa ttagttttag
    13621 aaaatgcaaa gccccgttgg ctttattttg cagtatcgtt tgttactttg cttttcatgt
    13681 attggttgtt agatagggag tatgaaaact tatataatag tacttataaa gaaagtgcgc
    13741 atttaagagt gcaaattgca gacgatttgt caaatttacc attatcctat ttttcaaaac
    13801 ataatttatc agatttatct caaactatca tgtctgacgt tgaaggtatt gagcatgcga
    13861 tgagtcatgc aatacctaaa gccggcggta tggctctgtt tttccctttt atttcagtga
    13921 tgcttttggt tggtaatgtc aaaatgggat tagctgttat tttgccaacg ttatttagtt
    13981 ttgtcttaat cttgttatca aagaaatccc aaacgaaagc caatactaaa tattacgata
    14041 ctttgagaga aaactcggaa gaatttcaag aaactattga attgcagcaa gagattaata
    14101 gctttaatct atctaaaaaa gttcaagaca gacttttcaa aaaaatggaa gagagtgaaa
    14161 ggattcattt aaaggtagaa ttaagtactt tttcagtcat ggccttatcc tctattttct
    14221 catatgttag tttagcagta gtcattctag taggtgttca cttactgtta acgggtgaag
    14281 tgactatact ctacgtcgtt ggttacttac tagccgcaat aaaaataaag gattccttcg
    14341 attctatgaa agaagcagtg cttgaaatat tttatttagc tcctaaaata cagcgaatta
    14401 gagctatgaa ggaaaccagt atccaagagg gatcagatag tcctctgaaa tcttttgaca
    14461 ttgagttgcg agatgtctct ttctcatatg ataacaatac cccaatttta gatcatatct
    14521 cttttacggc taaacaagga gaagtgactg cgttagtagg tgctagtggt tccgggaaaa
    14581 ctagtatctt gaaattagtg tctagactgt atgattatga tgaaggctgt attttaattg
    14641 atgggtatga tattaaacgt gtatctccag cttctttgtt tagcaaaatc gctattgttt
    14701 ttcaagaggt aactttgttt aatacaagta ttttggaaaa tatacgtatt ggaaatagtc
    14761 aagcgtcaga tgaggaagtg aaaaaagcag ctcgtttagc taactgcgaa gactttattg
    14821 aaaaactacc tgacggttat catacattgg tcggagaaaa cggaagttca ctttctggag
    14881 gagagcgaca acgcctatcc atagcacggg cttttttgaa aaatgcgcca attttaattt
    14941 tagatgaaat tactgccagt ttagatgcgg aaaatgaaaa acgtattcag gaaagtttaa
    15001 atcgtttaat acaagataag acagtactaa ttatatcgca tcgcttgaag tctattgaaa
    15061 aggtaaataa aattgtagtg atggatcaag gaaaactagt tgatcaaggt actcataatg
    15121 agttgtatcg aaggtcagaa atatacaaaa atctcataaa gaaaactaaa ctatctgaga
    15181 aattcgtcta tgaaaaagaa gctcaatcac gatgatgaaa taggctttaa attaacctac
    15241 aatttttggg atatagttat aattcttttt caaaaagaat aattttatta aacagcatct
    15301 aattttgaat acacgaaaga atcagtatga ttgaattagt tcacttaatg tctattctta
    15361 taatataagg aataatcaaa gttttaaaaa attggcgata aaccagttaa acataaaaga
    15421 ttgaataaaa agttatagtt tctttcatat tgccgtttta agtagaggaa aagctactta
    15481 aaacggtatt tgtgtttgat tggactaata aaagtgaatt cttatttttc agagcctaaa
    15541 ctgcatattc ttttaaatga tttgattatc tctatgtacc gataaattcg ttttcaaata
    15601 gttatctatt catatgagtt ttatttcatg ttatttattg ttgacaagag caactcttgt
    15661 tggtaaagta tcagtgtcaa aacgtaacga ttacgatttg aggtgtctaa atcaaaataa
    15721 aaggagtata aaggtatatg gcaaaaaagt cgaaaattgc taaagcaaag aaacaaatgg
    15781 cgatgattga aaagtatgca gataaacgtc aggaattaaa agccgcagga gatcgcaccg
    15841 ctctcgctaa attgccaaga gattcaaatc cgaatcgtct gaggcttcgt gatcagacgg
    15901 atggtcgtcc aagagggtat atgcgaaagt tcggtatgtc ccgtattaag tttagagaac
    15961 ttgcccatca agggttgatt cctggagtaa aaaaagcaag ttggtaaaag gaggaaatat
    16021 catggcagta ccagcaagaa aaacatctaa ggcgaagaaa cggttaagaa gaactcatca
    16081 aactgtagta aaaccagaaa tttcatttga cgaagcgaat ggtgattata gacgtagcca
    16141 tcatgtatct ttaaaagggt actataatgg aaagaaagta attaaaggag cttcaaaata
    16201 gtttaatata taacttttag gagggaatca aaatggaaat tatttaccca cctttagttg
    16261 aagaaggatt aaagtattat cttgaaacga cccagcaatc gctagataaa tgcacttttt
    16321 atcgttcgat ggtagagaga ggaattatta ctgaaacggg tttaccgacc caacaggcaa
    16381 ttgaaaatgg attagtcaaa gattactatg aggaccaagg cttatcattt gatgattttt
    16441 taagaatcta tccaattttt gaagagtatg acgaggagtt atttcaatgt atcgatggtt
    16501 attgggagat acctgttgat atgaaagaaa atttagtttc acaattggaa tctggcgagt
    16561 taacttttga agatgcacaa caaattcaag catatcttga agatcgataa gtattgaagg
    16621 aggaagattt atgcgtcaaa atattatttt ggaatgtgtc gaaactggag aacgtctgta
    16681 tctcaccagc aaaaataaac ggaataaccc tgaacggtta gaattaaaaa agtattcgcc
    16741 aaaattgcgc agacgggcaa tttttaaaga agtgaaatag ctaagtggta aaaaggtgac
    16801 aaaatgggca taccagttac aatcgtttct gggtttttag gggctggaaa aacaacgcta
    16861 atcaatcaag ccttgcaagt ctcaccctac cccagggaag aaattattat aattgaaaat
    16921 gagtttggtg aagttgggat tgatcacaaa ttattagttc atagcgagga acgggtgtta
    16981 caactaaata atggatgtat gtgttgcagt cttcgcagtg atttattagc aatattgatt
    17041 tctttactgg aaacaataga agaacatcaa caaccaattt cacaaatcat cattgaaact
    17101 acaggtatag cagatcccca accaatcatt caaacacttt taacagcgcc tcaactaaga
    17161 ggaagagtat atatcgatag tttattgact gttattgata gtgacagttt tgaacttatt
    17221 gaaagagaac cacaagccct taagcaactt gtcatggcag atcgcatttt tgtgtctaga
    17281 aaacaaaact tgaatttatc tgcactaaaa aaattacaga gagaaatcaa aagtattaac
    17341 cctttatccg atattcgaat gttcacacat agtgaaacga ttcaaaaaaa ggatttttat
    17401 aatttgaaaa aatttaatca tcccttagtt tttgaaagtg attcatcaga aaatcactat
    17461 aatggcaaat actatcatca acaccatcaa gtacatgagt ttaaatcaat tctcttaact
    17521 tcagatcaag acataaaaaa agatttttta ctccaatgga ttgactggct gattttaacc
    17581 aaccaaggga gcctgtatcg ttttaaagga ttgattgctt tacaagagca ggattttata
    17641 gttgccatgc aagaggtaaa cgaacaggtt aattttcaat acaccactcg acaaaaagac
    17701 caaaccacta ttattctaat cggaaaaatg ttggatgaag aaaggatcaa acagagtttt
    17761 gataaattac tggcggaaag caaataatga taagtagtga ttgggggtaa tttctaatgg
    17821 agaaaacgga tatatctagt gcatatcgac gacttaaaag cccgaatatt aaaactagaa
    17881 aaagggcatt aaaaattatc aaagaacata aacgaaataa aatgaagaaa ttagcataat
    17941 taaggaagga aaaagaatga agaaaatcag tgtaggactt attgggatag cagctctagg
    18001 attattggga gcttgctcca gcactaatga tgcaaaagta tcgaatgata aagatggaaa
    18061 attagaaatt gtaacaacat tctatccaat gtatgatttc accaaaaaca tcgtcggcga
    18121 tgaagcgaac gtggatttaa tggttccagc gggatcagaa cctcatgact atgagccctc
    18181 agctaaggat atggcaaaag ctcatgatgc tgatgttttt gtatatcata acgaaaacat
    18241 ggaatcatgg gtaccaaaag ctaaagaaag ttggaagaaa gctgggccta atgtagtaga
    18301 aggaacaaaa gacatgattt tattgccagg tagtgaagaa gaagatcatg atcacggaga
    18361 agaagatcac catcatgaac tagatcctca tacgtgggtt tcacccaaaa tggcaattaa
    18421 ggaagtttca aatatcaagg atcagttagt taaattgtat ccgaaaaaag caaaagtatt
    18481 tgagacaaat gctgaaaagt atttgactaa actgaaacgg ttggatgctg actataccac
    18541 tagtcttaaa gaagcgaaac aaaaaagttt tgtgacgcaa cacgcagcct ttggatattt
    18601 agctttggat tatggattga aacaagttcc tattgctgga ctaagtcctg aagaagagcc
    18661 atcttctggt cgattagctg aattaaaaga gtatgttaaa aaaaataaga tcaattatat
    18721 ttactttgaa aagaatgcta atgataaaat tgcaaaaact ttggccaacg aagctgggat
    18781 aaaattggaa gtactgaatc ctcttgaaag tcttacaaaa gaacaaatgg ataatggtga
    18841 agattatgtt tcagtaatgg aagacaatct aaaagcgtta gaaaaaacaa ctatggttgc
    18901 tggtgaagag gtagttccag aaaaagaagc gaaggatgaa aagacagttg ctaatgggta
    18961 ctttaaagat gccgatgtga aggatcgcga attgagtgat tatacaggcg aatggcaatc
    19021 tgtttatccg ttgttaaaag atggaatttt ggatgaagtt ttcgattaca aagcaaaaat
    19081 caataaagac atgacagctg ccgagtacaa agattattat actactggct ataagacaga
    19141 tattgatact atcaacatta aagacaacac cattgatttt gtagtcaacg gtgaacatca
    19201 tcaatacaca tacaaatatg ttggatacaa aattttgaat tatgaaaaag gaaaccgtgg
    19261 tgttcgtttt aattttgaaa ctgatgatgc aggtgctggt cgttttaaat atattcaatt
    19321 tagtgatcat ggaattgcac caagtaaggc agagcatttc catattttct ttggtggaga
    19381 aagtcaagaa aaactctata atgaattgca taattggcct actttctatc cagcttcatt
    19441 gtctaaacat gaaattgctc aagaaatgat ggctcattaa aattcaattt ttggaggaaa
    19501 tacatggtaa gaaaagtatt attgtctatg gattccaaga atattgatgc ttatcggcgt
    19561 tatcgatttg caaagcaaat gagattaaat gggagtacat tagctgatat tgctgcaact
    19621 ttacatgtta gtattgacac agttcataga gtattaaaac gggatcctga ttgttgtttt
    19681 atcaaggtca atactggaag aagtagacgt ttagcaaaac ggcataatag cttggccaat
    19741 taattgataa taccagagta gatcattttt cccttatttg gatttgatct gctttttatt
    19801 atgtcttgaa ttgtcaaatc aagcgcaaca ctttatcaaa gaagacttca taaggtgttc
    19861 tccaatctaa acactttcta ggtctgttat tcagtttttc aatcatctga tttatcgttg
    19921 agttatctac cgtagctatc tctttcgatt ttggcaagta ttctcgaatg agtctattcg
    19981 tattttaatt tgttcattgt tgccaagggc atgaggatct ggaaaataaa agggcatatt
    20041 attcaatgct tctgtaactg cgaaatgatt ggagaattct tttcctctat ctggagtgat
    20101 cgttttgacc gcctcatttg gaatactgct taaaagttca atcattttct cgctcacata
    20161 agcggctttc tttctgggga ccttctttcc tgctaaaagg aagcatgatt ttctgtctgt
    20221 caaagtcact aaacaagtac caccagtttt gccagctaca atatcggctt tccaatgacc
    20281 aaaatcactt ctatcatctg cggattttgg acggccatgg atcgaatgag taaggcaaat
    20341 ctttccacga gactcgacat gattttttgt atgtcttgtt tttcctcgat ggtgcaatga
    20401 acggatcagg cctttattgc cgtgtgagag gggttctgta tcgaaaagat cattgcaaat
    20461 tgcacgttag atcgtgataa aactaattga aaagtccata tgttcatgct tcaaacgatt
    20521 cgagatttcc tctggcgacc actgaaaatc gacaaataaa tgacgaacaa ttgaccaaac
    20581 tcacggatca tttagaatgt gataacgtcc gcattttttt cttcgataag cataattttt
    20641 ttccgctttg gaaggtgaat agtttttttc ttgtttcttc tcagttcatg taaaatagta
    20701 aacgggctat gctgaatgtc gttgccaatt atactaatag aaaatccttc ttcgtgaagc
    20761 agaaagattc tttctcgttc gagtatagta agatgacgat actggctcat aattattttc
    20821 cttctaggag tgttctcgac aaacttatac tagttggata tcgattatga ctcattttta
    20881 tgtgttgcac ttaaagtgta aattcaaggt atagaaagag gttccctatg aaaaaaatac
    20941 ctattacaat cttaactggc tatcttggtt caggaaaaac gatacttcta aatcatattc
    21001 tgtacggtga tcacggaaag gaaattgctg tcattgtaaa tgacataggg gaagtaaatg
    21061 ttgatggggt attagtcgac aagaaaggtt ttaaaagaac tggagaaaaa cttgtttcga
    21121 tgccgaatgg atgcatttgt tgtacattac gcgaagattt gattgttgaa ctagatcaat
    21181 tagtgcaatt ttctgacttg aatcaaattg ttattgaagc ttcaggaatc agcaaaccaa
    21241 ttccaattat tcaagcaatt atattagcta aaaacccttt agacattgat ttgacccaac
    21301 gattagagat tggtgccatt attatagtag tggatgtaaa acgtttttgg gataatttcg
    21361 aaacgggtga aaagattcag gaaagaaaag ctgaaggtaa tgtagttgaa gaagaacagg
    21421 atattcgtgg cttgttaatt gatcaattcg agtgttgtaa catattactt ttaaataata
    21481 gtgacttagt gtcaaccaag acgatttaca aaattaacgg tctaattgct aagttacagt
    21541 ccaatgccaa aataattagg acaattaaag gtgaagtaga cttatcgaaa attctaaata
    21601 caaatctttt taatatagat gagacttcct attcagtggg ctggttcaaa gagttagagc
    21661 ttggctatca aaatcatatt cctgaaacag aagaatatgg tatctcaagt tttatctatc
    21721 gaaatcggtt actccttgac gcacaaaaat ttgcccatct tacggataaa aattttcctg
    21781 gcaatattac aagagcaaaa gggtttcttt ggtttgccca acatagtgat ttttcgacga
    21841 tctttgaaac atcgggtagc tataaaagta ttgaatcatt aaattattgg gtagctagtt
    21901 tacctaaaat tgagtgtcaa atgattatac aaaaaaatcc cgaattgaag gaagcctggc
    21961 atccgttgtt tggtgaacgc attaataaac tagtttttat tggtatgaag atggatcaac
    22021 aatgtattaa ggaacaactt gagggttgtt taactgattc tcttaatgca gataatttcc
    22081 gaaaaatttc accatttcca tggaaaaagt ctaatatgtt tagccagata tcgttaatga
    22141 aatttttaag aaaagctggt gtcaagatag gcgatgtcat aattattggc attttgatta
    22201 ttacctcttt tattccctta tttgtattct ctatagaaaa ggagaatgaa acaactgaag
    22261 atgctatcaa atatgccatc gttcgaattg atggcaagga aatggatcgc ttcgatttgg
    22321 ataagatcaa tcataaactc gtgaattatc atcctgcgaa gggacaatat aatattgttg
    22381 agattaaaga tggaagaatt cgagttaaag aagataatag tcctgatcaa atcgcggtta
    22441 gaactggatg gatctcaaaa cccggtcaaa cgagtatttg tttaccccat aagttagtta
    22501 ttagtattga gaaaaaagaa agcacagatt actacattta ctaatctgga aatagatgag
    22561 gaggttttta ataagaaaaa gatattatta ttaataatag caggggcttt attaagtgtg
    22621 ttaagcgctt gtggacaaga aaaagaagcg gtgacaacag gcacaagtat ctttaacggg
    22681 acctgcaatg gtttagttaa cagtcggtta gattacttat tttggctctt cgtcaatgag
    22741 tgttggtgaa gaaattcaat agaataatcg agatactcac acggctagac agctatcttg
    22801 tctagccgtg ttgttgttct ggctttagtc agttgggtag tattcttagt tttcttaaaa
    22861 tattttcctc gatataaaaa gattcgttct cggtagtttt gaaagtttct atagccatag
    22921 gcaattcgtt ttagtacttt gatatggtta ttcaaacatt ctatagggcc gttggaatag
    22981 ggaacgcgca acgctcgttt gatttctgtt tggtatttct tgaaggtcgt gaacactggt
    23041 tggtagtgct ctggcaactc cttgtagtct tcttttagga gcgcgacgaa ttcaggaaca
    23101 tcttgtcttc tgataacgga aagaaaatcc tggtagactt cgtagcctcg ctttaaggaa
    23161 tcatcataag ataatagccg atcgactatc tctgcttctg tcagatgatc tcgaaatgac
    23221 ggacgccagt agcgatgctg acctttcaat tcccacgcat tcttttgaag tagtttccag
    23281 tatttcttta agtgcttcga ccgacgatcc ccttttttaa aggtcttcat tacttgaatt
    23341 cggtggttgg taaaagcccg accaatgtgt tggctaatat gaaagcgatc ggcgacgatt
    23401 cgggcattcg gaaaacactc tttcaccaat gtgcgatagg gtgtatagaa atctgaaacc
    23461 actagacgca cttgttcacg gacttttata gggtagcgca agaaatagtc gcgtaaagga
    23521 ttgagttgcc gattttccac aatatcaatc agttgatggg tcttgccgtc catcatgaca
    23581 aagctcatgg ctcccgagac atttttcact gatttgaatt catcgaaaca caccacttct
    23641 ggtaacagct ggctcaaaga aatggtttta ggccgaaggc tgcgaagtat tcgaatgaca
    23701 gtggtcggag aaatgtgctt catacgtgcg atcaaagaca tagaaagagg ttcgcttaat
    23761 agctcaagaa tggcttgttt gactctgcga gcaatggagc agcgacgatc actaactgaa
    23821 tcttctgcta aaaaagtctt tccacagtgt ttacatttga agcgctgttt gttaatccgc
    23881 agaatggtct tgtagttact gacatcattc aataagatgg atactttttt ccagccctat
    23941 ttaatgatcg tttgttcatt ttgatagtgg cgatgaaagc attcctccgg ttgataggtc
    24001 aatgtgccat caaaaacaaa acatgtttcg tcattgattc gctctttgcg aaaggcatct
    24061 tcatgaaaga taagatttag gtctagaata tctagtaact ctttggtaaa ctctgaatag
    24121 gacatcttga aacactcctt tgaatggttt tcgtcgactt tattctaaag gattttaagg
    24181 tgtctgtttg cgtttattag aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca
    24241 ctagatatta tagatccctt attttcttcg cttcaaattg tcaaggagtc atcataaatt
    24301 ttgtatggat tcttttgttt gggtagtagt cttctaaata atcttggatt aacaccttca
    24361 tctctatttt acttttatat ttgttggagt cgattaattc tcatttgatt gatcgatgaa
    24421 acgcttcaat tactgcattg tcaacaggag tcccagtttg gctcactgaa tgtgtgatgt
    24481 ggtggtcgtt caacagctct tgataaataa aagaccgatg ctggctacct ctatcggaat
    24541 gaattaccgt aggcaattct ccttaataaa tttgtaatgg acggacgaca atctcttttt
    24601 tcatgtgtga atctaccata tagtaacgta gaatacgagt tgctaagtca atataggtgg
    24661 acaaatagag gcgaccgtca ttacatggaa tgtaagtaat atccatcacc caaacagcat
    24721 caatttggtt ctgttcaaat tatcgattca acattttttt accatttctt tgattgtttt
    24781 ttctctacca tgagaagatt tgcgtcgatg aaatcctctt gtgcataaat tcatttcacg
    24841 cataagaaca gctacaacac gtttattgat ttcgattcct tcatcgtaaa gtttttgagt
    24901 gatccctgga gatccgtatc gatctttgtg ttcgtagaac actcgtcttc ctcttttagt
    24961 cgtttatttt cctgttcaag aatgtagaaa tctgctttat caggtaatct ttttccttgt
    25021 ccagcaaaag catttttccc gtatcgacga tattctgaaa cccatcggga aattgttgga
    25081 cgagagatat tgtattcttc agcaattttc ttggtagtta cttttttctc aaggatatcc
    25141 tgtgaaatct ttagtttaaa tttacgatca tatgtttttc tcaatagagt tcattccttc
    25201 cggtaacgta actgatagtc aagaaaagtg tatcatagcc caacttagta tcagggtttg
    25261 tcaactaaac tgtggaagtt gatagataag agtttttaac aaccaaaatt ctcttgacta
    25321 tttttgaaat cagacaaatt ttgattggac acatagttga ataaatctac cgtcgttacg
    25381 gaaggtaaat cgcatgcttt tcaattctag aggagaagtc ctcgttgaag taccatagcc
    25441 cagttctgga ttcgacctcc tgcccacttc atacgtaaat aaagtaattt taggagtgag
    25501 actgtgacaa aagttatttt taagctaaaa acgaattaaa aaacgttgat aattaaaatc
    25561 attcgtttta gattctatga atcattatag aacgctgtaa aatcgatact ttataattat
    25621 aaagaagata gtttcaaatt tattgttcga aatttgaaac tatcttttta cttatgtcac
    25681 agactccctt agttgagtta ttctttaatt tcgaatttcg agatagctag atatattgca
    25741 agcatacatt cttgataaca atcttgatca tattttccag ttctccagga ttgcttatga
    25801 atcaaagagc taaatctttt gagaattgcc tcaacagctt cttcatttcc agcctttgct
    25861 aaaatgaaat cttcttttaa agaattcata aatctacctt cttcccatgg acttataatc
    25921 tatttctttg ggaaggagga gaacgagcaa agaagcatac aaaattagtt attatgcaga
    25981 tattaaacca gcattttttg aatttttttg taaagtcgat gttttaaatt tgtcgcaccc
    26041 tgtcttgtaa tccccagaac ctttccaatt tctttatcag ttttatcaaa gataaatttt
    26101 tcgataagaa aaatttgttc aggttctttc aacaaaggaa ggatttttgc taattttata
    26161 tcttcaacaa ggagagtcac atttaaaaca ttaatcggtt gtttaaattt tatagtatct
    26221 ttagtaaaca aatatggtgg gagttgatca agttgttcat gtttttgaat ttcttcttgt
    26281 tttttataat aagtattagc ggtgttcaaa atgacctttt caaagaatcg aatcaactgg
    26341 gcatttacgc gttcgtcgtt atctgacata ttgcacacct cctccattaa gatttaaagg
    26401 gaaaagaggc aaaaaacacg aaaagaagct aatgcaatta tgctgcgctg cgaataactt
    26461 ctataagctc acatgcattg ataggactgc tgatgtttat tttcaaaaat gtttttttag
    26521 ttgataatgg atagttatct ttatacttag aaaagaaaat tcatatatct tcttaaatat
    26581 caaacatgtc cttatggcta agaaaatgtc ttgagctttt tacgtataat aaatcttttc
    26641 taaaacctcc ttttggataa tattcttatt cgtgtataga ttgtattttt tatttcttcc
    26701 cccttcataa ttaacaacta gaaaagagtt cgaatagaca cgtatttgaa cgaaaatttt
    26761 tattggattc ccaaattgga atatttagaa acgaaaaatt cctattatct gtatagtgct
    26821 tagataataa cggatcataa taattattgt ctttgaacta gatatatgta aatagtattc
    26881 atcatgatga aaaatgatga gttctcggtg ataaaatgta ctttttacgg tggaatatac
    26941 atgaaagcga taagtggata ttttataatg aagtaagtta gcttattttg acagaggaat
    27001 agtattttag ataacgtata aaatactatt taatgatgta gaaaggaaag agagaaacaa
    27061 aagtgttttc agataaagaa gtcttattaa agtctcttca tgtttgttta aagttaccta
    27121 tcgtggtttt taattccgaa ttcagtatta taaaagagta tcgctcagat agaactactt
    27181 atttttttta tgattataaa aagttactaa ctgataatat taaaaaaact aaagatttcc
    27241 atttctttac tggtgatttt aatgagatgt ttcttctata catgtttaaa accagatatt
    27301 acttatttgg tccatttaga gcaaataata ttgataaagt tttttttaaa ctaaaaatga
    27361 acaatttgaa tgttgcaatg gctgatagag aacgactcta taattcattg caaaatttga
    27421 ctttgtattc tttaggagac attcgagata ttctgatttt agttcattat ttttttactg
    27481 gaaaaataga agatctattc catgaaccgt taattgaata tacagaaaat ctaagtaaaa
    27541 ctatcgagca aatcaaaata gataatctgt tatcacaaaa ttacgatcca gaaatatatc
    27601 tgtttcttta tgaaaataaa atactggaat atgttaaaaa tggagatatt cgaaatttag
    27661 aaaacatggt ttttaatctg agtaatggaa taattccaag tgttagtgga gatacgattc
    27721 ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg tctataacat
    27781 taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa gaaaacgaat
    27841 tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt tattatacta
    27901 aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct gtcgttcaat
    27961 tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat tatttttctg
    28021 taagtgaaac taaattaaga gaaagcttta agaatgaaat gcatattacg atttataatt
    28081 atatctctaa gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac catactatca
    28141 gtgaagtttc tttaggttta ggattttcag attcttcaca cttttcgaga gtattcaaaa
    28201 agtacgcagg tgtatctcct aagcaatatc aattaggttt agtggacaat ttcaacaaca
    28261 ttagctgaga ggttttattt tattatgaga ctatatcatt tacgtagttt ggaggagttg
    28321 aggaaattgg attttaacaa aaaatttttt tgggaaattt ctgatttgag ttgtttcgta
    28381 gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga caaagtagta
    28441 attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca tattaatggt
    28501 agcaatttta aacaaacatt atttaatctg attaagctag atgctaaaat atcaagttat
    28561 ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca gttaattgaa
    28621 agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt aaacaactac
    28681 aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta gagtactaga
    28741 cactttaaat atggacaatt caattataaa gaaaaagaat caataattta atgaggctgt
    28801 gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata actccaatca
    28861 ttcgtttaag attctaggaa tcattcagaa atgctgtgaa accgatactt tataattata
    28921 aagaaggtga tttcaaattc cgaagaagga atttgaagtc acctttttac ttatgtcaca
    28981 gtctcattca gtaatgcggg ataaggctcc ttattccgtt atttgaatac tctgaagttt
    29041 aatctaagaa agggtaatat tttgaaaaca tgcttcatcg catagaaatg aacttaattt
    29101 cagaaagaat acatacatat ctttttatat gttttcagct gctgaatggt taaaaagcac
    29161 aaatatccga aaaaaacatg aagaaatgta ctttttttgt tgtaatgttc atgaaattct
    29221 ctaagtaaat tagttaacat aaggttagta agattcctat tctgtgaata atacgtatca
    29281 gatttatctt ttattcttac taggaaagta attattaagg gagttgattt gaataatggt
    29341 tagcaagaat aataagagag tatttctaga aaaaacaaag aaacgagtat tgaagtacag
    29401 tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt ggtgttggtc ttgtcttggg
    29461 aacaactgaa ctagttaaag cacaagatga aatttctcca agcagcccac ttgaaacagc
    29521 gataagctcc gtacaagtag gtgacaaagt agcttcgggg aatacttttc aggagaatcc
    29581 tggttatacg aaaaattata acttttcgga tttacaattt agtcctcaag aactaactgg
    29641 agacacacta aaaggcaata caattggatt tgaagtttac ggaaaacata atattgcagc
    29701 atcaactaaa aactgggaaa tccgtcttca attagatgaa cgattagcta aatatgtaga
    29761 aaaaattcag gtcgatccaa agaaaggaat tggatcttct agacggacct ttgtaagaat
    29821 taatgattcg ctgggtagac ctacaaatat ttggaaggtc aattatattc gtgcaagtga
    29881 tggattattt gctggggcag aaacaaccga tacgcaaacc gctcctaatg gggtaattac
    29941 gtttgaaaag agcctagatg aaatttttaa agagataggc atagacaatc tcaagaccga
    30001 tcgtttaatg tatcgaatct acttggtaag tcatcaagac gatgataaaa ttgtaccggg
    30061 tattgatagt acgggctact ttttaacaga ttcagatgat ttctataata gcttagatgt
    30121 atctgaaaat aatcctgacc agtttaaaca tggatcagta agtgcaaaat atgagaaacc
    30181 taatactcaa acaaaagatg gttcaggatc cacaggtgct aatggagcta taatcttaga
    30241 tcataagtta acgaaaaatt ataatttctc atattcatct tccgcaaagg gcacgccttg
    30301 gtatgctaat tacaaaattg atgaacgatt agttccatat gtagcgggta tacagatgca
    30361 catggttcag gctgacaaag taacatatga tgtttcgttt gaatccggaa caaaagtagc
    30421 tgatttagca atcgagcgac gggaaggtca tgaaaattat ggcataggtt caataactaa
    30481 taatgattta actaaactta tcgattttgc taatgcaagt ccacgtccag tcgttattcg
    30541 atatgtttta caattgacta agccattaga tgaaatctta gaagatatga aagcaacagc
    30601 acaagttgaa gaaaataaac catttggtga agatttcatc tttgattctt ggttgtcgga
    30661 tacgaataaa aaattaatcc agaacactta tggaacaggt tattattatt tgcaagatat
    30721 tgatggtgat ggaaaccctg acgataaaga agagagcgga gacacgaatc catatatcgg
    30781 gaaacctgaa ttagaagaag tatatgatgt tgacacaaca gttaagggga aagtattcat
    30841 ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt gataaagaag gtactgtatt
    30901 agcagaaaaa actatcgctc caaatgaaaa agatggggct ccaatttcag atactgtaga
    30961 atttgaattt acgggtgtag attcaagtaa attaatcgcg aaagatgaat taaaaatcca
    31021 aatcgtttct ccaggttttg ataaaccaga agaaggttca accgttatta aggaatcacc
    31081 aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa ccagatgcta aagaatcaat
    31141 tcggaataat aaaaacttac ctgaagatgc agagtattca tggaaaacag agcctgatac
    31201 ttctaacgtt actgatagta cgaaaggtat tgtaactgtt aagatcggaa atcgaacttt
    31261 cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg atgcaacata
    31321 cgtacctata acaacaactc cagaaacgac agttcaaagt ggaaaaccta catttgataa
    31381 accagatgtt cctttagcta aagatgcctt ttcaatttca gatgtctata ataaggactt
    31441 cggtaatgca agtgttgatg caaacactgg tattgttaca ttcactccag ctaaaggtgt
    31501 aggagaatcg gagccgatta ctggaacaat tcctattaaa attgtttacc aagatggttc
    31561 tgtaggcacg accgatttag cagtaactgt aagtaaagat atttatgaaa atccaggaga
    31621 aaacattcct gcaggctacc acaaagtaac cttcaccgca gg
//
LOCUS       DACEKG010000030        30587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEKG010000030 DACEKG010000000
VERSION     DACEKG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.030"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..226
                     /locus_tag="IW664_001284"
     CDS             <1..226
                     /locus_tag="IW664_001284"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0848656.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IW664_001285"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IW664_001285"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IW664_001286"
     CDS             533..1336
                     /locus_tag="IW664_001286"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0848657.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /locus_tag="IW664_001287"
     CDS             complement(1585..2649)
                     /locus_tag="IW664_001287"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0848658.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="IW664_001288"
     CDS             complement(2646..3731)
                     /locus_tag="IW664_001288"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0848659.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IW664_001289"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IW664_001289"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0848660.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IW664_001290"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IW664_001290"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0848661.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5980..6633
                     /locus_tag="IW664_001291"
     CDS             5980..6633
                     /locus_tag="IW664_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0848662.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            6718..7623
                     /locus_tag="IW664_001292"
     CDS             6718..7623
                     /locus_tag="IW664_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0848663.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="IW664_001293"
     CDS             complement(7625..8257)
                     /locus_tag="IW664_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0848664.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="IW664_001294"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="IW664_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0848665.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="IW664_001295"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="IW664_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0848666.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="IW664_001296"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="IW664_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0848667.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10137..11375
                     /locus_tag="IW664_001297"
     CDS             10137..11375
                     /locus_tag="IW664_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0848668.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11423..13552
                     /locus_tag="IW664_001298"
     CDS             11423..13552
                     /locus_tag="IW664_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0848669.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13527..14216
                     /locus_tag="IW664_001299"
     CDS             13527..14216
                     /locus_tag="IW664_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848670.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14624..15310)
                     /locus_tag="IW664_001300"
     CDS             complement(14624..15310)
                     /locus_tag="IW664_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0848671.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15446..15640)
                     /locus_tag="IW664_001301"
     CDS             complement(15446..15640)
                     /locus_tag="IW664_001301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848672.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15690..16130)
                     /locus_tag="IW664_001302"
     CDS             complement(15690..16130)
                     /locus_tag="IW664_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0848673.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16134..16979)
                     /locus_tag="IW664_001303"
     CDS             complement(16134..16979)
                     /locus_tag="IW664_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0848674.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17128..18546
                     /gene="pepV"
                     /locus_tag="IW664_001304"
     CDS             17128..18546
                     /gene="pepV"
                     /locus_tag="IW664_001304"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0848675.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18622..19824)
                     /locus_tag="IW664_001305"
     CDS             complement(18622..19824)
                     /locus_tag="IW664_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848676.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19811..21598)
                     /locus_tag="IW664_001306"
     CDS             complement(19811..21598)
                     /locus_tag="IW664_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848677.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21611..22564)
                     /locus_tag="IW664_001307"
     CDS             complement(21611..22564)
                     /locus_tag="IW664_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0848678.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22749..23099)
                     /locus_tag="IW664_001308"
     CDS             complement(22749..23099)
                     /locus_tag="IW664_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0848679.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23299..24378
                     /gene="pepA"
                     /locus_tag="IW664_001309"
     CDS             23299..24378
                     /gene="pepA"
                     /locus_tag="IW664_001309"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0848680.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24521..24841
                     /locus_tag="IW664_001310"
     CDS             24521..24841
                     /locus_tag="IW664_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0848681.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24863..25327
                     /locus_tag="IW664_001311"
     CDS             24863..25327
                     /locus_tag="IW664_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0848682.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25523..26128
                     /locus_tag="IW664_001312"
     CDS             25523..26128
                     /locus_tag="IW664_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0848683.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26167..27456)
                     /locus_tag="IW664_001313"
     CDS             complement(26167..27456)
                     /locus_tag="IW664_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0848684.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27884..28363
                     /gene="rlmH"
                     /locus_tag="IW664_001314"
     CDS             27884..28363
                     /gene="rlmH"
                     /locus_tag="IW664_001314"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0848685.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28716..29030
                     /locus_tag="IW664_001315"
     CDS             28716..29030
                     /locus_tag="IW664_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0848686.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            29046..29432
                     /locus_tag="IW664_001316"
     CDS             29046..29432
                     /locus_tag="IW664_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0848687.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            29461..>30587
                     /locus_tag="IW664_001317"
     CDS             29461..>30587
                     /locus_tag="IW664_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0848688.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         9686 a         5721 c         6239 g         8941 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttctttagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcaa gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
    10561 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
    10621 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
    10681 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
    10741 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
    10801 gggtcgttct tttagatctt caaatcatct taggttttat tctagcaggt atctgggcat
    10861 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
    10921 tatttacaga tggcgcagtc gatgttgcac caatggttgg tttcttgatg accttagcga
    10981 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
    11041 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
    11101 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtgttagctg
    11161 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaattaca acgattgctc
    11221 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
    11281 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcggt gcgatcttat
    11341 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
    11401 tagataaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
    11461 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
    11521 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
    11581 aaattgtttg cgggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
    11641 cacacaagcg actaatgcac tgattgaagg agatgtagca aaagtcggcg taatcgggat
    11701 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
    11761 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aaaacttaaa
    11821 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
    11881 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
    11941 atctgtgaaa gcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
    12001 aagaagaact cggacagcag caatcaacgc atctatttta ccaaaaatgc tagatacggc
    12061 aacctcgaca gaagactccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
    12121 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
    12181 ttccggtcct gctgcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
    12241 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
    12301 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
    12361 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
    12421 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
    12481 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
    12541 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgttct
    12601 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
    12661 acaggcatta gcagactatt gtcatacgac ggttgaagac atcgcagagc agatcatgga
    12721 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
    12781 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
    12841 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
    12901 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
    12961 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
    13021 accagaatcg atcgaagtcc acgtagaaat cgatccacat acttcaaaag taacggctat
    13081 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
    13141 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
    13201 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
    13261 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
    13321 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
    13381 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
    13441 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
    13501 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
    13561 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
    13621 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
    13681 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
    13741 gagaaatcta tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
    13801 aacgaatcac ttttgcaact tatgcagaag atgaagggat ccgattgatg acggagccgt
    13861 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
    13921 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
    13981 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
    14041 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
    14101 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
    14161 aagaaataaa agaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
    14221 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
    14281 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
    14341 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaaaggtg ttcaaaagct
    14401 tgcatctgcg gtaataagaa cagccaagtt aaaaacataa gaagctattt ttacttggct
    14461 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
    14521 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
    14581 tatttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
    14641 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
    14701 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
    14761 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
    14821 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
    14881 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
    14941 cttttttatt ttgagcgatc actgcctgat cggtttgttc tagtattttt tctagtttag
    15001 cttgatcttg cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
    15061 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    15121 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    15181 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    15241 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    15301 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    15361 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    15421 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
    15481 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    15541 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    15601 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    15661 atacaagaaa aatgcttaga cggtatttat taagaattta catcatccaa agaagcgtac
    15721 gcttggaata actgttctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    15781 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    15841 cccacaattt cgtagctaaa atccaaccgt gttcccgtat acttttggat agttgggtgg
    15901 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    15961 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    16021 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    16081 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    16141 tcgttttcca ttgatttttt acaaaaataa ttcatccact tcggaagtgc ttcgctgatt
    16201 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    16261 taaacagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    16321 atcccagcta aagtgtcacc catgccgcct gttgccatag cagcagaacc taaaggattg
    16381 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    16441 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    16501 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    16561 aaaagatgat tagcaacaag taaacttaaa gcagagccat caatcaccag ccattgttct
    16621 tttgattgtt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    16681 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    16741 gtccaatcta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    16801 tcagttacta cagtcgtgag acccgctcca ctgtgtacgg cagctaaagc actcataatg
    16861 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    16921 tgtgaaatct ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    16981 gaaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    17041 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    17101 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    17161 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    17221 gattcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    17281 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    17341 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    17401 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    17461 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    17521 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    17581 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    17641 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    17701 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    17761 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    17821 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    17881 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    17941 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    18001 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    18061 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    18121 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    18181 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    18241 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    18301 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    18361 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    18421 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    18481 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    18541 aaataaaaat actaaaaaac tccggctatc tctgacagct ggagttttta tgtttaaaaa
    18601 tgaattagta catcggtatt cttaaaagta atttagttcg tcttttgatt tctcaggagg
    18661 gatcaaaaag gcgaaagcac taagtatagc aaaacttgtg atgatttgcg tacgatacgt
    18721 aaaccaataa tgaatctgtg agtgattagc taaaatattg taccaaatat acggcgttac
    18781 agcagctaaa acaaacaaaa ctagaagttg gaacggacgc cagctatttc gtttacgata
    18841 agcaaaatag agaaaaacaa gacaagtaat caatagcaat aatattgcta cttttggata
    18901 catcaatcta atattgcttt tcagcatctc taatcgatgc aacgggtatt tgtcgtttcc
    18961 ttccgttcga aagaggattt ggttgattgc atcttttaca atgtttttgt tcaatatgat
    19021 cgatgatagg atccatttag ccacccatgt caaaccatag cctaatcccc aaaagatacc
    19081 ggtaaaaaca aaactgccta cttgtttgct caatgaactg cgtttatcca ttaaatactt
    19141 atttgttaca taataaataa tgatcacagg tatcccccaa gagatgaccg gtgttgtcaa
    19201 caagtcaaaa aagttagcta aagaaccaat gacaaagaaa tacaagaata attgttggtt
    19261 ggtgttggag agttgtggtt ttgttaaaaa tagtaataca gacaataaag tcaaaatcca
    19321 aatatttgaa aattgcatac tgaagaaaaa ggttgcagca tttcctatag caagcgaaat
    19381 caacaaggct gtcgttaaat agatgtcgct acacttggtt aaaaagtagc tcgtcaatcc
    19441 aatcagcaga aaaagaatgg ctgcgtaaat cattcgaata ctccccaaac tcatccctag
    19501 cagtaaaggt cgtaaaaaaa gctgataacc atgccaataa cggggataat ccgccataga
    19561 catcgcgttc tccaaggagt tcaattcagg atctgctttt gtttttcgta tcatcaaatg
    19621 atcagtaaaa ttatctaacc gagtgcctag catatttcct tgattgatag aaggatacat
    19681 tccttctttt tcaaccatct gtagagattt ttctgcattc gattcaatag agtgctgcgg
    19741 aatgaaaaaa gtaattgtca gtagtacgca atagctagcg atcaagagaa caaaagcaaa
    19801 aaatatcttt ttaaatagtt tcatgagtat ctcctatatc tgttttcatt ttttctcttt
    19861 tatctagtaa aaaaggagag aggatataaa caggtagacc taatgctaaa acaaacacat
    19921 accgtaaact aaaagccaca ggagtagcaa tcataatagt agcaaaaaca agcataccgg
    19981 gaagaaaaag gagtaaatac cgatattttt tcttaaagat tgctaaaagt gcactgccga
    20041 tagctaacca cagtaatgtg ccggaaccaa agaaatcacg agcatcgatg atttttttca
    20101 ttcctgacaa tccagtaaga tgttcaatca aatctacttg cttcatacca taattattcg
    20161 tgtttacacg agtatctaaa aagccgtaac tattttttgt accaggtacc cagaacccga
    20221 acgtttctaa aatataagca tctgtataga tttttatatt acttggtaaa tttcttgccc
    20281 atagtgatag aaattctttt ttatgtgtat tcaaaaactc attgttaaaa tttttatgcc
    20341 acttgattga gtctgcaaga ctaggagaat acgcttcata atcttttatc gggagtagct
    20401 gattcatgaa tgctttatct ttcttagaca agtcaccatc cataacaata gttctagaaa
    20461 tttgctgcaa aggaacacct aattgttcag cagtttctgt aggaatagca taacgagcca
    20521 tcaacgggtg gatgagtagt ccagcaatca ctgtataacc cagcaccact tgaaagaatg
    20581 cttgatacct tttttgtttc aaaataatca agccgcaaat caaacctgtg ccggttacta
    20641 cataaatccc attatttctt agataggtgg tcagtaaagc aaggatgatt acgtaaatct
    20701 gattgagaat cgttggcttt tctcgttgaa aaataaagtc aaaaaccgct aaggaaaata
    20761 aaaagcaagc gatagagaac aaagtatctt tttgcatgtt caaggcgtag attggaaaaa
    20821 ccggtgcaaa tgcaaaatag cataaactaa tgagtaccgg aaaaaaatgg atgtttcttt
    20881 ttaacagcca gcaaagagaa taacttatcg ctgaagctgt tatcaatgtt tgaaaaatcg
    20941 aaaataaaaa tacaccgtga ttgatcgtat cagttggtaa atcatgaaca aatagcttga
    21001 ccagtaaggt gaataagact ggatgatgat tgcttatagg aacatcaccc atagattggg
    21061 cgaccgaact taacgaatca ggcaaaacca ctccaggata caatgccaac agataaggaa
    21121 gccaacacag aactaaaaag acagtaaaga ataaaaaata ttttcttgaa gagaaagcct
    21181 gtgtgttttg agcagtaaaa aatgtttcca gttttggcat tgataaataa atcgattgta
    21241 aaaataaaaa agaatacaca aaaatggaga aataaacaag aaagtaaaaa atacttccat
    21301 cagcaatcgt tgtctttgta tagtttgctg agacatactg aaattgtatc tgtctgccaa
    21361 tagaaagtgt aagagcaaaa gctgcagaaa aaaacaatcg ggcaatagct gcaactttgt
    21421 tttttattga aaacaactct attccatatt gcttaaaagc aagtagcaaa accatggcta
    21481 aaccaatgat tactgttgga ttcaaagttc ctagaccata agaagtaaaa aaagcaaaac
    21541 ctgacgcaaa taaagttgca aaaaaagtag gaaatagatt ctcctttttc ttatgcataa
    21601 aaaaggcctc ctacttcatt tctttatttt gatattcgtg gtagacatgg tataaattca
    21661 attcatattc tttcttggca tttgtgacga ccgtatccaa aatcaaccct gttactaatg
    21721 aaagaagggc taaagtcatc aatccagtac ttaaaatcgc agaagggatg cgtgtaataa
    21781 agcttgtcag catatattca cgaatcacag gaatccccat gaccaatcca aataaaaaga
    21841 agaagatggt ccagatccca aagaaaagca aaggtttata atctttgcac attttgatga
    21901 tcatcatgat cactttgaat ccgtcagaga atgtattcaa ttttgattcg ctaccttcag
    21961 gacgatcgcg ataatcgatt ggaatctcaa cgaacttgaa ttttttatct aaagcatgaa
    22021 tcgtcaattc tgtttcgatt tgaaaaccag aactcataat agggaagctt ttgacaaaaa
    22081 ttcgattgaa tcctcgatag ccggtcatga catcgcgaat ttttgtttta tataagtaag
    22141 taattgtatt tttaaccaga ttattcccaa aatcatggaa aggacgctta ttttcatcaa
    22201 aataggttcc atttgataga cgatcgccaa tgaccatatc tgcttcgcca gaacgtaatt
    22261 tctcaagcaa accatgtgca gcttctgcag gataagtatc atcaccatct accatcaaat
    22321 aataatcggc atcaatatca aaaaacattt gacggattac attccctttt ccttgacgtg
    22381 gttctttctt tacgattgct ccgccttgaa cggccaattc gtatgttcga tcggtcgaat
    22441 tattatcata gacataaata tctgcttctg gtaattccct tttaaaatct tcaataacag
    22501 ttgaaattgt cgcctcttcg ttataacaag gaatgagcac ggcaattttt tcacgtgatt
    22561 ccatttgtca ctctccttat ataataaaac taatcttatt tcaataatac cgtataataa
    22621 ctacaaatac tagctattcc aagaggaaat agccctattt ttataaaaat cacccactat
    22681 aaaagaagga taggatacct ctcttttaga tagtgggtgc gtattcgtta tttcattttt
    22741 ttgacgtatt aatctattgc gccctttttt cttgaaatgt ctaatacggt tcctgtatga
    22801 gcatctgcta aaaattcata ataaacagtt tcgccatctt ctatccggga aagcccaccc
    22861 cgataagcac tcgaccgaac ggcgaatttc cgaataggtt ctttttcaaa agagatccaa
    22921 gcaccttcga ttgcgccttc ttttaaaaaa gatgttttga tattttcaag cacttggtct
    22981 gctgataaca cttttttgcg atgataccaa atcgttgaag ctaccccact caataatcct
    23041 agaccgatcc caacggctaa cccgccttta aaatagctta attcgctttc ttcatacatg
    23101 tggatatacc tccttcttct cacttgctac tctttgtgtt tattttacca cactctcata
    23161 ttttctttga aataaataac cgctaccaga aaaaataagc tataataaat acgatcggtc
    23221 tgcagaaaaa atcatatttt atccgcagat caatataagt ctgagaggag tttagtctga
    23281 aatttagacg aatagcatat ggaagaaaaa acatttcaac gaatcaaaga gttaacagaa
    23341 ttacaaggaa caagcggttt tgaacaagac gttcgcgcct atatggaaag tcatatggaa
    23401 ccgctggtag atgagttaca gttagatggt ttgggcggca tttttgggct gcgccaccat
    23461 gaagaagcgg atgcacctcg tgtaatggtc gcagcacata tggatgaagt cggattcatg
    23521 ctgacacaaa tccaagaaaa cgggcttttt aaagtcgtac ctctaggcgg atggaaccct
    23581 tacgttgttt ctgctcaacg ttttacactt aaaacctcta ctggtaagaa ttatccatgt
    23641 atttcttctt ccgttccccc acatttatta cgaggaacaa gcggacaaaa atcagtagaa
    23701 gtaacagata ttttgttcga tgctgggttt gaatcaagag aagaggcaat gagttacggt
    23761 gttcttccag gagacacaat cgttccttac gctgaaacga tcaaaacagc caacgggaag
    23821 aatatcatca gcaaatcgtg ggacaaccgc tatggctgta caatggtatt agaagcttta
    23881 gaagcgttac aaaatgaaac attaggtcat acattgattg caggcgcgaa tgtccaagaa
    23941 gaagtaggct tgcgtggttc taaagcttct gtcaataaat tcaagccaga tctgttcttt
    24001 gcagtagact gctctgcagc agatgacaca gtgacgaaaa atggcacatt cggacattta
    24061 ggagaaggta cattgatgcg tatccaagat ccaggtttga tcatgcttcc tcgtttgcgt
    24121 gagtacttgc tagatatcgc tgaaacaaac aacatccctt atcaatactt tgtttcaaaa
    24181 ggcggtacag atgcaggtgc tgctcataca caaaatgaag gaatcccaag tacagtgatt
    24241 ggcgtagtgg gacgttatat ccatacgcat caaacgatgt tcagtattcg cgattttgaa
    24301 gctgcacgag aaatgttgat tcaaacattg aaaggtttag ataaatcaac agtgaacacg
    24361 atcgtatacg ggaaataacg cgatagtgaa actataaaat ctggcaaaag caaccttttg
    24421 tcagattttt tttataaaaa gaggatcaat gtttgttagt tcgaaagaaa ggtgtataat
    24481 ttaattgaaa aatacataaa cagaaagtag aggaaaaaga atgattatcc caaatagttt
    24541 agaagaatta gcaggctacg tagaaactgg aaaaagtgta tttttcttta ctgccggctg
    24601 gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa atcgaaaatg acttttctga
    24661 ttggcaattt attgaagtag accgtgacaa gtacatagat gtagccgctg aatggaacat
    24721 ttttggtatc ccaagttttg tcgtgatcca agacggaaaa gaattaggtc gtctagtgaa
    24781 taaagatcgt aaaacaaaac aggaaatcga atcgttccta gaaagtgtaa taaaaggctg
    24841 acatctgtta ggaggggtca agatgaatca agaatataag aacattttag taggtattga
    24901 cggtagtgaa caagcacttg ctgctttcaa aaaagcgtta gaagtggctc gccgtaataa
    24961 tgggaaagta tatgtagcaa acgtaatcga tcagcaattt tataatttta tgggctatgc
    25021 tcctattgat caaaatctag tcgatcagca aactgaaaca gccaagcaaa tgatcgaaga
    25081 atgtaaagaa tatggaaaat ctgttgatta cacaaatata gaaggaatta ttgcatacgg
    25141 ctctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa tatgcaatcg atctaatcat
    25201 ggttgggcag tctggactaa atgcagtcga acgctttatg acaggaagtg tagccagcta
    25261 cgtcatacgt cattcacctt gtgatgtctt gatcgtttct gacgaagaag aacagccaag
    25321 taattaagca agtggaactt gtataacctt tttaatcaaa cagtaaaggg actgtgacaa
    25381 aagtcttgtc acagtccctt gttacgaata aatggtgttc cagaagaagc ttcttcggaa
    25441 ataaagcatg aaaaaaatct catgcagtga atgcaatttt ttgttaagat aaatctcagc
    25501 aaaggataat ggaggaaaga acatgatttt tgcttataac aaagcacacg tcggagacac
    25561 tttgatggtt atcatagccg acgataaagg aacagaaaac actgtcgaac gtaaatggaa
    25621 tgttgctcgg atcaaagatg aacaaggtca agttgttgct tggaactttt tccatatctc
    25681 cgatcatcta acaattgaag ggaacggcca agtaagctta acagatgaag aattagcgaa
    25741 attgaatcga ttgattaaag aagcaggatt cgaagaacag cttgaagctg atcatgaacc
    25801 gaagatcgtt gtcgggtatg taaaaacatg cgtcccgcat cctgactctg accatttatc
    25861 gattactgag acagaagtgg acaatgggac agtattgcaa attgtttgcg gtgcaccaaa
    25921 tatcgaagct ggacaaaaag ttgtcgttgc aaaaccagga gccatgatgc cagatggaat
    25981 gatgatctgg ccaggaactt tgcgaggggt agccagctat ggaatgattt gttctgctcg
    26041 tgaattgcat ttgccaaatg ctccagaaaa aaaaggaatc ttagtattgc ctgaggatgc
    26101 cgtagtcgga gaagtatttc caaaataaag aggttgtgac aagtctttgt cacaacctct
    26161 ttattattaa ttttcactat tttgattttg ttttaatgcc gattgatcga tcgtcaattc
    26221 aacagtagtt gttttttctt tcgagccttc atagtaagtg attttgacag aatctcccac
    26281 ttttttctta tacaaagcag attgaagttc tacaccagaa gaaacttctt tgtcatcgat
    26341 cttagtaatg acgtcgtatt gtttcaaccc agctttttct gccggtgtag ctgtttggac
    26401 atttgtcacg atgacaccgt tagttacaga tgaaggaatc ttcaagattt gttcttgttg
    26461 ctgagtagaa acggcagata agtcgaccat tgtgatacct agagctggac gtgtcacttt
    26521 tccgtctttt tccagttggt tgatgatatt cactacatcg ttacttggga tggcaaatcc
    26581 cattccttct acgcttacat tagaagtcga ttcagaagta cttgcgatct tacttgagtt
    26641 gataccgata acttgtcctt cgatattcac taaaggacca ccagaatttc ccgggttgat
    26701 cgctgcatct gtttggatcg cattgatatt gactgtttcg ttcgattcat tcgtacttgt
    26761 tacttgtcgg ttcaaggaag aaatgattcc tgaagtgaca gaattcgcat attcagatcc
    26821 taatggggaa ccaatcgcaa tcgcaggttc accaaccttc aaagcacttg agtccccaaa
    26881 agaggcaacc gtttcgactt tgtctgaatt gatcttcaac accgctaaat ctgaataggc
    26941 atctgttcca actaattcag cttttacctt cgtaccgtct ttcatcatga cttccaaacc
    27001 ttgctgtcca tctactacgt gattgtttgt gactacataa gcagtattcc cgtctttttt
    27061 gtagattacg ccgcttcctt cactataagc ttccaaatta ctatcatctg agctgctact
    27121 ttcttcttgc tgcccgaata atccgccaaa accgcttgat tggttttgac tttgtaagtt
    27181 gataacagaa acgacagcgt cttgaacttt atctaccgct ttggtgatat cagaatctac
    27241 attgactttg acattttcca caactgtttc accggcagaa ttttgattgc cgcttgtagc
    27301 agtagaacta ttgttgccag tgcccatcgc tgcataaaaa atgccagctg tcagtagtcc
    27361 tccaacgatc cctccaacaa gccccagacc caacttacgc cagaggctat tatttttatt
    27421 ttttttcatc tttggtgtca cattttttct atccatgtgt tcctcctcca attacattca
    27481 tttatctact gttagtatag tcttatttgt tgactttagt ctactttgag actttgaact
    27541 ttttatgaaa aactttagga aaaatcattg ttttcagatg aaaatatttg attttctttt
    27601 tccacagata ttgtgtaaaa ctacgcggat aggattggaa aaataaggga taaaacatat
    27661 ccacagcctg tgtataaagt ggataaattc ttataaaacg tatgtttgcc taatgaatgt
    27721 gatgaaataa ggtaaaacaa cgctgttttc tgtggataaa cctgtggatg ttgtgcataa
    27781 ctgttgggga aatcttttta acaaaaatag tcaaaataaa catatagacg aaaaaattga
    27841 ttataataaa caaagtgagc gtgaagatca aaggagagaa aacatgaata tcaaaattat
    27901 ttcagtagga aaattaaaag aaaaatactt gatccaaggt atcaatgagt atgtcaaacg
    27961 gctgaatgcc tatgcgaaaa tcgaattgat cgaagttcct gacgaaaaag ccccggagaa
    28021 tttaagcgaa gcgcaaatga gacaagtaaa agaaaaagaa ggagaacgga tcttagcgaa
    28081 aatcaaagaa caagaatacg tctatggctt agcaattgaa gggaaaaatc ccacaagcga
    28141 atcttttgct aaacaaatcg accaattagg tatccaagga aaaagccacc ttgtttttgt
    28201 tatcggcggt tctttaggac tcagcgaagc cgtcatgaaa agaagcaatg ctcagatctc
    28261 ttttggcaaa atgacgtatc cacatcaatt gatgcgcctg atcctcgtcg agcaaatcta
    28321 ccgtgccttc cggatcaacg cgggggctcc ataccacaaa taatgatatt ttttaagaga
    28381 aaaatagcat aaaaatctag ttatccgcat aaaaactgga cttatcacac tttatcaagg
    28441 tcaaaaccac tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt
    28501 tatccttgaa aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta
    28561 gacacttcaa aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat
    28621 gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc
    28681 attcagccaa taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca
    28741 acatggaaaa aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa
    28801 gaatcaacgg acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa
    28861 gagcagatga tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg
    28921 aggaacgtgt gaagttagtc aatccacgta ttaccgcaga gggctacaaa atcggcactc
    28981 gtggttttac aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag
    29041 gaaaaatgat gagattagca aatggcattg tattagataa agacacgact tttggagaat
    29101 tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag
    29161 atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag
    29221 taagtattcc tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta
    29281 tcaatcccat tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt
    29341 atatcaaggc agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac
    29401 aagcaaagaa agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc
    29461 atgaaacagc gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg
    29521 atagcgtcac tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc
    29581 cagcagatca actggcagga ttttaaccta tcacaagcag ataagattga cattccctat
    29641 ttaattatca gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa
    29701 cgggttcgct atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata
    29761 cttgaaaaca agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt
    29821 gctggtcgta caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat
    29881 ggcttgatac agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac
    29941 ttggaaaaga aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat
    30001 tcctatgtgg aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa
    30061 gtagaagcta aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag
    30121 ctccctcata tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca
    30181 ctgattgaag ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct
    30241 gaccttgcgg acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt
    30301 tcttgcattg aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg
    30361 aagaactata agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc
    30421 tttgatgaat acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg
    30481 aataagctga aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct
    30541 tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc gtgatca
//
LOCUS       DACEKG010000031        30460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEKG010000031 DACEKG010000000
VERSION     DACEKG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.031"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..554)
                     /locus_tag="IW664_001318"
     CDS             complement(<1..554)
                     /locus_tag="IW664_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0848689.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(680..2608)
                     /locus_tag="IW664_001319"
     CDS             complement(680..2608)
                     /locus_tag="IW664_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0848690.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2914..3630)
                     /locus_tag="IW664_001320"
     CDS             complement(2914..3630)
                     /locus_tag="IW664_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0848691.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3631..5454)
                     /gene="glpO"
                     /locus_tag="IW664_001321"
     CDS             complement(3631..5454)
                     /gene="glpO"
                     /locus_tag="IW664_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0848692.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5468..6964)
                     /gene="glpK"
                     /locus_tag="IW664_001322"
     CDS             complement(5468..6964)
                     /gene="glpK"
                     /locus_tag="IW664_001322"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0848693.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7100..8555)
                     /locus_tag="IW664_001323"
                     /pseudo
     CDS             complement(7100..8555)
                     /locus_tag="IW664_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(8545..9840)
                     /locus_tag="IW664_001324"
     CDS             complement(8545..9840)
                     /locus_tag="IW664_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0848694.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10172..10849)
                     /locus_tag="IW664_001325"
     CDS             complement(10172..10849)
                     /locus_tag="IW664_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0848695.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10879..11970)
                     /locus_tag="IW664_001326"
     CDS             complement(10879..11970)
                     /locus_tag="IW664_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0848696.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12071..12505)
                     /locus_tag="IW664_001327"
     CDS             complement(12071..12505)
                     /locus_tag="IW664_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0848697.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13089..14546)
                     /gene="ascB"
                     /locus_tag="IW664_001328"
     CDS             complement(13089..14546)
                     /gene="ascB"
                     /locus_tag="IW664_001328"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0848698.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14543..16438)
                     /locus_tag="IW664_001329"
     CDS             complement(14543..16438)
                     /locus_tag="IW664_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0848699.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16496..18271)
                     /locus_tag="IW664_001330"
     CDS             complement(16496..18271)
                     /locus_tag="IW664_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0848700.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18321..19817)
                     /locus_tag="IW664_001331"
     CDS             complement(18321..19817)
                     /locus_tag="IW664_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0848701.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20011..21633)
                     /locus_tag="IW664_001332"
     CDS             complement(20011..21633)
                     /locus_tag="IW664_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0848702.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21961..23274)
                     /locus_tag="IW664_001333"
     CDS             complement(21961..23274)
                     /locus_tag="IW664_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0848703.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23519..23743)
                     /locus_tag="IW664_001334"
     CDS             complement(23519..23743)
                     /locus_tag="IW664_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848704.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23763..24131)
                     /locus_tag="IW664_001335"
     CDS             complement(23763..24131)
                     /locus_tag="IW664_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0848705.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24327..25037)
                     /locus_tag="IW664_001336"
     CDS             complement(24327..25037)
                     /locus_tag="IW664_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848706.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25055..25552)
                     /locus_tag="IW664_001337"
     CDS             complement(25055..25552)
                     /locus_tag="IW664_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0848707.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25589..26041)
                     /locus_tag="IW664_001338"
     CDS             complement(25589..26041)
                     /locus_tag="IW664_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0848708.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26176..26781)
                     /locus_tag="IW664_001339"
     CDS             complement(26176..26781)
                     /locus_tag="IW664_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0848709.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27231..27851)
                     /locus_tag="IW664_001340"
     CDS             complement(27231..27851)
                     /locus_tag="IW664_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0848710.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27853..28722)
                     /locus_tag="IW664_001341"
     CDS             complement(27853..28722)
                     /locus_tag="IW664_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0848711.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28745..29152)
                     /locus_tag="IW664_001342"
     CDS             complement(28745..29152)
                     /locus_tag="IW664_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0848712.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29171..30460)
                     /locus_tag="IW664_001343"
     CDS             complement(29171..30460)
                     /locus_tag="IW664_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0848713.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8631 a         6626 c         5021 g        10182 t
ORIGIN      
        1 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
       61 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
      121 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
      181 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
      241 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
      301 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
      361 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
      421 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
      481 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
      541 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
      601 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc cactgatatt
      661 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
      721 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
      781 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
      841 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
      901 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
      961 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct tttttattct
     1021 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
     1081 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
     1141 caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat ttaggacgaa
     1201 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
     1261 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
     1321 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
     1381 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
     1441 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
     1501 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
     1561 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
     1621 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
     1681 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
     1741 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
     1801 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
     1861 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
     1921 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
     1981 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
     2041 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
     2101 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
     2161 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
     2221 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
     2281 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
     2341 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
     2401 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
     2461 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
     2521 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
     2581 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
     2641 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
     2701 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
     2761 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
     2821 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
     2881 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
     2941 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
     3001 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
     3061 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
     3121 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
     3181 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
     3241 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
     3301 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
     3361 aatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
     3421 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
     3481 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
     3541 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
     3601 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
     3661 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
     3721 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
     3781 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
     3841 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
     3901 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
     3961 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
     4021 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
     4081 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
     4141 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
     4201 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
     4261 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
     4321 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
     4381 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
     4441 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
     4501 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
     4561 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
     4621 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
     4681 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
     4741 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
     4801 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
     4861 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
     4921 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
     4981 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
     5041 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
     5101 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
     5161 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
     5221 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
     5281 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
     5341 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
     5401 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
     5461 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
     5521 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
     5581 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
     5641 gcgccgttgt ttccaaattt tctgctcgtt ggatcggtac attcaagata tctgtttgga
     5701 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
     5761 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
     5821 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
     5881 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
     5941 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
     6001 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
     6061 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
     6121 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
     6181 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
     6241 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
     6301 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
     6361 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
     6421 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
     6481 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
     6541 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
     6601 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
     6661 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
     6721 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
     6781 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
     6841 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
     6901 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
     6961 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
     7021 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
     7081 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
     7141 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
     7201 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
     7261 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
     7321 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
     7381 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
     7441 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
     7501 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
     7561 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
     7621 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
     7681 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
     7741 cgtctactat cgaaaaagaa acaaaagagc tgtaaaacat cattgtttcg tatacattca
     7801 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
     7861 ggtccgactg atagagtaat ttctttcaaa gttgcgtacg tacttttttc attcaatagt
     7921 ctttttttcg taatcccgag ccagcaagca atctgtgctt ctgctgatgc cgtaaacgtt
     7981 gtttctaata cacggtccaa tcctagaatg aacggtcttt tttctggtgt attttgcaaa
     8041 aaaagcggtc gctgatcttc cgggatatat tggaatagtt cataataaaa atatcgtatc
     8101 tgtagctctt ctccgtataa ttgaccgttt ttgatttgta atccaaattc ctctaagagc
     8161 tggttcaatt ctttgatctt tcgaaaaaca gaagactcac tgatattaaa cgctgtcgct
     8221 aatcgaacga tggatacttg ctgatgttct aataaataga cgagcatctt aaacttcaag
     8281 ctttcctgca ataaaaacaa tagaattttt tccaaactta catcttcgtc caacacaagc
     8341 agcgcctgat actcatttcg ctgtacttct actttttttt ccatcattcg gccaatcaat
     8401 tcaatatctt ccaaatattg atcaaacgag gcttttgaca gttggacatg ctctctcaaa
     8461 ccattgatag aggctgttcc cccttgcaga acaaggtttc gcaaaatcag gatctgtaac
     8521 tgctcttttc tttctaataa tgcttcaatc ttcataggca gtattcgcca cctttgacca
     8581 caccatatct ggcacacggt attctggctg gaaaaatagc tcatgttctt ctaactgttc
     8641 aaacgtccat ccttctttta ctgcaattgt cagttggtca agaaggtgga acatctcttt
     8701 tttgctttgg agctgtgctc caagaatctt tttttcagaa tcaaatagaa atttgagttt
     8761 aactttgctt tgatttattg ctgtaagctt gtagtctacg tatcctgttt caattttttg
     8821 gggataaaaa atgctctctt cttctgtcaa acctacgctt cctaagaaat agccaaacca
     8881 ctgattgccg actggacgat aggttccctg atcctttgtc tgtgagccac taattgtctt
     8941 gctgacattt cgtgctgtac ggacagcgtt atttactaga gaaacataca ttttttcatt
     9001 tgttggacga aaagttactt ggatcgcatc gccaatcgca taaacgtctt tttgagacgt
     9061 ttgcaagtag tcatctaccc agatcgttcc atcatcattc aatgttaatt gttcttccca
     9121 catctgattg tcggggcgag aatgattagc cagcaaaaca agatcatagt tctcctttct
     9181 tttttccgtc tctacaatag cggtttcatt ttcggtcaaa gacacaactt gttcttctaa
     9241 aaaaagtgaa agcccttgat ttttcatttc ctttactaac ggttctgtca tttcaggatc
     9301 tagataacga aaaagaattg actttcggct ttcgtaaaga tggatttgtt tttttcttga
     9361 tgccaaccct tcagctattt cgattcctgc ttgtcctgca ccaataattg caattttctt
     9421 tgcattcatg atcttttgct gaagagccgt tacgttttgt atctttttaa ctgggtgaat
     9481 aagcgttgat tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg ctagaatcaa
     9541 acgatcaaaa tccaattttt ttcctcctgc taaaacgact tttttttctt tcaatcctat
     9601 tactgtcttt tctgtgtaaa catggattcg atagcggtca gtcagttctt ctttcgtgat
     9661 ccaagaaagt tgtttttcac tggtaacctt tccttttaat atcaaattga acccgctggg
     9721 aatccatcct aacttcgatt gcttttcaat caaatatatt tcacttgttg gcatcaattg
     9781 gcgaagataa atagccgcat gaatccctgc aaacgacccg ccgacaatta caatgttcat
     9841 ctgcattcct ctttcttata aggtgttcta ctgctaactt ctcaatcaga aaacacagta
     9901 ttcataactc gctttcaacc ttatgataca tagcgatgca gcagaatcaa ccctctccat
     9961 cataccgtgt tctaggtctg cttctcaata atgtactagt ggacctaatt gaagagtatc
    10021 atttctgaag agatgtttca aaaaatgtac tcttataaaa gaaaaaaact cgccttttac
    10081 gtacctctgt ccaaatagga aacagaaaag aacgaaaaga cgagttttag cgtgtgtaat
    10141 gtaatagtta gaatagacac atcatttatt tttaattctt taatgcttca acaactgctt
    10201 cgcctaattg atgagcagat tgaggatttt gccctgtaag aagccgccca tcaatttgaa
    10261 ccacttctga aaaagctgct ccagactgat agtttgctcc tttttctttc aaagcatctt
    10321 ctgctaagaa tggtacttca ttcgttgtac catttgcttc ctcttcttca ttactgaacc
    10381 cagtcactgt acgtccagct aaaatagatt ttccagactc atctgtaact ggcaacaatc
    10441 ctacaacacc gtgacaaaca gctgagacca gcccttcatt tcgatagatt tgtgaagcga
    10501 ctttcccaag atctattgct tctggaaaat cccacattgt tccgtggcca ccagcataat
    10561 aaaccgctgc ataatcattt gcatccacat catcaggacg taaggtattt cccaaggccc
    10621 gatttctgaa atcttcatct tcataatagg cccaatcgat ttcatccata ttcaaaacac
    10681 tttgcggttc taaaggtaca tatccgcctt taggactgac aaaatctgca tcgatcccat
    10741 tttttactaa tacatcataa aaatgagtca gttcacttaa ccacaaacct gtaggacgct
    10801 cgatcttttc gtacttttct acatttgtta atacgatcaa tactttcatt ttcccatcct
    10861 ctttctataa aaattgcgtt attgataacg tgctttcaag aaattatata atgggcttgg
    10921 caaataaagc tctttatatg cttcttcaaa aggtttcgtt acaaattcat cctcacggtt
    10981 ttccaataat ttctctgccc agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag
    11041 ttctgcatat ttcaatacct gctctacatc ttcgcgattg cggatcccac cgacaccaac
    11101 taaagggatc tttccttgaa tcgtgttatg gaccacttca agaatcgatt tatcgttgta
    11161 tttatcagaa cgagctattc gatcatacat atttaatgaa acatgcagat aatctagttt
    11221 tgactcacgt aatcgttcaa gaagccagat cgtgtcttca aaacggatac ctggtgtttc
    11281 atattcttct ggtgagaaac gataccctac gatgaatggt tttttagcat attgttcaac
    11341 cgctgagaaa accgttgtta ataattcttc aataaaacga taacgatttt ccaagctacc
    11401 gccccattga tcctctcgtc tattcgaatg cggagagaaa aattgttgga tcaaatacgt
    11461 atttgctcca tgcaactcga ttccatcaaa tcctgattca atcgctctac gtgtggcttt
    11521 gccaaatgct tggattgttt caacgatttc ttcttcactc attgctcgtg gtgtttccgc
    11581 atcaggacgt tttgctgcaa ctgcacttgc agatacaggc tgttctccag ataaagttgc
    11641 tcgacttgtc attcgtccac cgtggaaaat ctgaataatc gcttttgcgc cttctccttg
    11701 gattgctttc gctagctcac ttaatcgagg aatcatttca tcatgagcga tgctgagttc
    11761 acctggccag ccttttccga tatcttggac atttgcagca ccagtaataa cagcaccaag
    11821 acctttagaa cgttgtttat aataagtgat ctcatcttcg gtgatcactc cattgaaaaa
    11881 gctgagttgt gtcgtcatag gcgacagcat caatcggttc ttcaattgaa ctcctgaagg
    11941 taaagtaatt gattgatcaa attgtgacat tgtcattcct cccataattc gctctgatta
    12001 acctatcgag aatcatctat cattaaacca ctcgattagt tgtacatgca actaatttgc
    12061 ttaaaaaatt ttattttttt tgtaacttat ctgttgcatc attcattgtc tgggctaatt
    12121 ctttacttgc ttcatttccc aaaactgctt tgtatgactg atgaagttct tcccagctac
    12181 catcgacttc cttagcaaat tggcgtcctt gttctgtaag ataaactaat gacaagcgac
    12241 cttcgctaac tgttttgacg tatcctttat attctaattt ttcaacaaaa cgagcaatcg
    12301 ttgatggggc ggaatagagc atttgtgcaa tatctttttg tggtattcca tctttttctt
    12361 ttaatatcaa taaaataaaa ccataagttg gtgccagacc tgtccttcta aacgcttcat
    12421 cagctaattt attcaacatg cgatctagct ttttcacgct aaagaaaaga cattcatcta
    12481 acgctttttc aattggcttt tccatttcat cccctccatt ggcgtacata tcatagtatg
    12541 atttttattt tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct agatttgaaa
    12601 aaaagaagta gccatcttca aagaatttcg gcttgttttc gacaaaactc cttctggaac
    12661 accgtgaatc agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa aaattttgtg
    12721 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgaaaaact ggcttttgaa
    12781 caccgtttat atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa
    12841 acaaaaatag ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg
    12901 tacaccgttt attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt
    12961 tcaaaaaaca gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg
    13021 agaatactgt ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt
    13081 tccttttatt ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat
    13141 tttttcttta accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat
    13201 ccataacgtt ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac
    13261 gtataaccaa ataaatctac tccatctttg atagcttcct tcatctgctc aatatgctgc
    13321 ttcaaatagt caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata
    13381 gctccgagtc cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc
    13441 aaagcaattc tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac
    13501 ggatttttaa ttcctccagt tattaagttt ccttggaccc cttcccaatt ttctggatga
    13561 gcactgactg cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct
    13621 aagatttctt catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt
    13681 tgcgtatacg ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt
    13741 tgctgctcaa ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga
    13801 tagctgatca tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct
    13861 aaagcacttg ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga
    13921 tggttttctt caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca
    13981 ttgaaagtca tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa
    14041 cgaacgtaaa actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat
    14101 aacggcattt cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg
    14161 tcaaacactt gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga
    14221 ggaaaaattc ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat
    14281 aaacggatgt cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa
    14341 tacttgctgt tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga
    14401 tctgtccatt tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat
    14461 cctttttcat ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat
    14521 aaaaatcctt ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt
    14581 gattattttt cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa
    14641 ccattgtggt cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata
    14701 aatcgccttt acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta
    14761 aattcactgt atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc
    14821 caattgcatg tttcgttgga aaaactgttg aaacttcccc gtttacagaa ctgtagattt
    14881 ttccgtccgt tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat
    14941 cgtttacttc agataaatca attatttcac ctgctgcagg agtacttaac actatatcct
    15001 ttgaggattc ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac
    15061 tgatcatcgc taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg
    15121 ggaaagacaa aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta
    15181 ctccaccaaa tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag
    15241 tcacaccata aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag
    15301 ataaactttt tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt
    15361 gcgcagctac ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat
    15421 tgttggtcat tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa
    15481 atccgccgat aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag
    15541 ctgcaatcag ctgacttgca taatctgcaa ttggacctac aactaataaa ataattggga
    15601 ccatcacgat gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt
    15661 tgcgaatctt aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg
    15721 tacttgtata ccttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac
    15781 tatatgcact gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac
    15841 gatctgtatt gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa
    15901 atgcatcagc tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca
    15961 aactcgtaca taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag
    16021 tcggggcaaa aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag
    16081 ttcctctctt tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat
    16141 acacttcatt tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag
    16201 tgatcacacc ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg
    16261 taaagcgtaa ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa
    16321 atcggacaat atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta
    16381 atgccgtagc aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc
    16441 aattagtacc ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc
    16501 cgagcaaatc actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg
    16561 agacaaaaaa aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat
    16621 actctgtgat ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat
    16681 ttaacggaag cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct
    16741 ttttatggat ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct
    16801 ttttttcttc aactatggtc agcttttcac gcaaaatctg ataaatctcc caagattcaa
    16861 actcatctag aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct
    16921 cagtagtcaa aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca
    16981 ctggttctat ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta
    17041 gtgtattcaa aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa
    17101 aaggattcaa cgcttcggca agatacaaaa caaaataagc gctttcagct aaattgggac
    17161 gtaattgaaa atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg
    17221 agatgatctt ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca
    17281 tttgtttgat atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat
    17341 ctaaaaaacg aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt
    17401 tttcatcatt aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt
    17461 cttgattgga aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca
    17521 tcggaataaa taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg
    17581 tcaaaacgag ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg
    17641 atataagaac ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac
    17701 tgatttgttg aggaaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga
    17761 aaattctttt tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca
    17821 gttctaattt aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg
    17881 ctgtttttga aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa
    17941 acaataagta cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt
    18001 tcgtcacctc ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac
    18061 ctgaacgttt atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga
    18121 tttcagtttg gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta
    18181 tccaatgatt cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca
    18241 tttgcttaca cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt
    18301 cacttgcaga ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta
    18361 gactggataa tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg
    18421 ctcccatcat tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt
    18481 cctgccgata cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct
    18541 tttattgcct cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg
    18601 tggattcgat tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat
    18661 aatggtaatt cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca
    18721 attggccaac tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt
    18781 gctccgccag tatctacgcg catttctgct tcttttttat agacagcgct acgataataa
    18841 ctaaatgcaa tataatctgc cgtgttagct tgcaagcact gttcgtctcc atctaacatt
    18901 actggtgcac agtcttcttc cttccatgtc ctgtaaatat aagctggata atgccctcgg
    18961 cacatgacat cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc
    19021 tctggatcac agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc
    19081 ttagcatttg gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata
    19141 tgatgacagg cttgataaat cacttttttt gttagtgtag caatgggttc aagtttatct
    19201 ctagcaggga atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc
    19261 tcattaaagg tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca
    19321 taaaattcaa aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat
    19381 gctaaggggg tttcataatg actaagagta attactggct cgatatcata tcgatttaat
    19441 gtattgatta gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct
    19501 cttggaaata ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc
    19561 agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa
    19621 tggagtgatt cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta
    19681 ggatgctggt tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc
    19741 aaccatgcac cttcacattg attagcagct actgctccgc cccataaaaa attttctgga
    19801 aatatttcct gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata
    19861 gctcgattca tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga
    19921 atgctactct tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga
    19981 aaacactcgt taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata
    20041 aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac
    20101 ccttaaagct tcacgtggaa tagtgaacgt ctgcttttca ctggacatat tacaaataac
    20161 gagccattgc tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc
    20221 cactaattca tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt
    20281 cttgtacata taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt
    20341 ataatttgga ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc
    20401 gttccattgc ataggcgtcc tagcattgtc tcttcctttc gccttgattg attccaagat
    20461 ttcctctttt gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc
    20521 ttttgcttcc gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata
    20581 gatataaggg gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt
    20641 tcgatacaca tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca
    20701 aaataaacta ttccatcctt catcacctaa agatgtttgc catttggata atactttttt
    20761 cagttcagag atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa
    20821 tcctatgtgt tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag
    20881 tttagcgatt tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc
    20941 aaaagttgct tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat
    21001 ttctttatcc ggttctttgc caatcatatc gatcacgtcc atacgaaagc ccccaatacc
    21061 tttatcgatc cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca
    21121 attcaaatca ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc
    21181 caactgccaa gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac
    21241 cggatcacgc caaatataat agtcccgata aggattttct ttatttttac gggcctcaat
    21301 gaaccacgga tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt
    21361 tcttttcttt gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat
    21421 gattgcttga taattactga tatcataccc attgtcatca ttcggagact cgtatactgg
    21481 agaaagccaa attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc
    21541 ccgcaaatct ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata
    21601 aacaactgaa tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc
    21661 tattttttgc ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat
    21721 agctattgca ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc
    21781 tcaactgttt tttgaaagga ttgatttatg cagggaagaa tgctgatttc acaacttctt
    21841 tataaacggt gttcactcag ctgtcccttg gtattagctc aaaaaacggc aaaatataag
    21901 aaactatttt ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct
    21961 ttatttcaca ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat
    22021 tggagatact tcttttcctc ttttagctgc taacatggta attggataga agataaaacc
    22081 taatgcaatc ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga
    22141 agggatcgca atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac
    22201 tcctaccata atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga
    22261 aaagaacaag cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc
    22321 tccaacggcg ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa
    22381 ggaacccgca agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt
    22441 gttgtctttc atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa
    22501 gaaagtcaca agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc
    22561 tacatgtttt aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc
    22621 agaaggcatt ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat
    22681 gaagatgcca cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat
    22741 cgtcaaccaa gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga
    22801 aacgatgatc ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga
    22861 aatcgcatat ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat
    22921 tgtgatcaaa ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg
    22981 aatccccatt cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc
    23041 cgttccaatt ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa
    23101 tgcagtcgca gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt
    23161 tacaaataag atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt
    23221 taccgtggtg ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca
    23281 tccccctaaa tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac
    23341 aacgtgtttt atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc
    23401 aaaatccgaa cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt
    23461 tggattacaa gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc
    23521 aatagtgtga tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat
    23581 cgacaggatt cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt
    23641 ttgtcttctt ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc
    23701 gccgatactt caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt
    23761 attcaatcag acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa
    23821 ttgaaagtca agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag
    23881 atagtttcaa attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt
    23941 gaaaacttct gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg
    24001 agtacaatac tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt
    24061 ttttctacaa agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt
    24121 ttttgtatca tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat
    24181 tccattatat cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc
    24241 aatagtttct aggattgctt tcctccacaa acttttttta caaaaaaaaa acggcaatga
    24301 atgccgctct tcttatttcg attcttttat aattctgctt ctgattcatc tttggttagt
    24361 gtgtgtctaa cactagttac gccaaatgtt tgatcgattt tttccttcaa cgcttcgatt
    24421 gcctgtgcgc cacgtgccgc ttgttcagca ttgatggatt caatgaccag caccgctgct
    24481 tgtgtttctt ccgtcagcat cgttctttgg gcttcacgcc agttgagaca gcgacagatc
    24541 gctccttgtt catcatagta gatgatttct tccggaagtg ccggagcatc ggaatctgca
    24601 cctaaaggca ggaatgattc gccaccttgg gcttttccta aatgcagatt tcctgcgatc
    24661 ttttcgatat cttctccgcc gcaaggtacg ccaaaagaca aggaaatact attatagata
    24721 tctacaagcg gattgattgg gaaaaattcc cgttcttgat ttgcacgttt cagtaaagct
    24781 tcaatagaag aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg acgccactga
    24841 gcaatgactt cgttttggct gaatgtttct tctgtaaggt aatttcttgc ttcttttgct
    24901 gcctcattca ataattgatg atacgtttct tcactttctt ttgttacgtg gttatcgata
    24961 ccatttacgg ataagatttc gatggttgct tcaggaaata cttcccagaa attggtatcc
    25021 actattactt ttttcatctg ttcacacctc ttgattagaa taattgcttg atgaatagat
    25081 agtctgtttg gggatcatct cccatataaa aaacatgttc gctgtatttg ataaatcctt
    25141 ttttacgata aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc caaatggctg
    25201 ttttttgctt ttcttttgct ttttcaatag ctgtatctag taaaaatgta ccgatacctt
    25261 gatgtttaaa tttttttcgt acatagattc gctcgatttc taaaaaatga tcagccattt
    25321 cctcagattg actcgttcct gtatttagtt tcaggtaacc cgcaggctct tgatcgatac
    25381 tcactaggaa aaaatcggtt tctttatctt ttagctcttc tgtaagtttt tctagtgcat
    25441 aagcttgtgt caagtaatca gctaaatgct ccggtgaatt atcttttcca aatgtatctg
    25501 taaatgtttc gatactaatt tgttgtagta agtctacatc tgaaggcgtc atttttcgaa
    25561 tttcgtattt attcacggct tcttcctttc aatagatcct tttattgcct tttttgacaa
    25621 aattccaatc ttcgctaaca ttcttgcata ctttatttaa aagatattcc aattgttttg
    25681 cttcttcgtc agaaagacca tttaatgcga ctgcattgga gtagttgttt tctcgaatga
    25741 tgaaaggata gatttctgct ccttttttag tcggatagag tttcttgatt tttttatttt
    25801 ctttatcttc caaacgttcg atcatcccat tggattctaa tttcttgatt gctcgggcag
    25861 tcgttgtacg atctactttg atcatttctg ctagtttttc ttggatgatt ccaggatttt
    25921 cgcaaatacg tactaaataa agatactgtc ctcttgttaa atcgacttct ttgaattcga
    25981 tattgctaat agaatctagc gctcgggcaa tcattccgat gtctcgaagg atttcagcca
    26041 tttcatccca cctttatttt gtttgtaaga ctagttcacc tctttttgtt gcaaatgcaa
    26101 ctattttcaa taaaaaaatc aagttctttt ttaataacta gtaaagtata ttcttttctt
    26161 cttttacgaa aaagctcaat cacaatagat ctggtttttg ttttctaaaa taagtaactg
    26221 tttcttcgtt gttcgctttt ctgatagctt tggcaactct tctatactaa aaaatcccat
    26281 gttggaagtt tcattattct caataaactt ggcctcttca ccgtggatcg cacaagcaaa
    26341 aacaagttta taatactgaa aaagctgtgg aatatctttt tgtttattcg tatcaaaaac
    26401 tgctcgcaac tgagtagttt ctactgttaa tcctgtttct tcatagactt ctttacgcac
    26461 attttcttca ggagacaatc ctatttcagc aaatccacca ggaagtgacc attctttcgt
    26521 ccgtttatct tcgattagta ggaccttgcc atcttttttg atataggcac gaacatctac
    26581 ttttggtgtt ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt cgaaagtttc
    26641 ttttcctatt tcactgatca gttccaatga aatcgtccgg atctcctcaa aacgctcttt
    26701 atcaaataca tcttttccat aatatagacc agcttgagaa agtgcgagta gtcttttgta
    26761 ttgagccaac ttcttgttca ttctcttccc ttctttttta gcaaatgtat gactatgtaa
    26821 tcaagtatag aagactggtt taattacggc aaataaatat aatgaaatct gcaataaata
    26881 aaagaatgtt aaaaaactcg agaacaaaag acattcgact tctctggggc tgtgaataaa
    26941 tggcgttcaa aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa tatgacgagc
    27001 tattcttgtc tggctgttct tattaattga ggcaggacag ctttttcaca acctccccac
    27061 taaacggtgt tctcctacca actcctcggt cacaagtcac aatattcatc aaacatgaga
    27121 agctgttttt ttgaaattgc tccttgtgac tcggagctga acgacaggct cacgacctct
    27181 cggtaaacag cgttcaaaag ccaatttttc gatattaagc catttctttg ttacttattg
    27241 tcattgattt ctttttctaa tttatccaat agtggagtgt aatccacttg agtatagtct
    27301 gttacatcaa attcatatag tgttcctaac tggaatttgt catattttcg gtcggcgatg
    27361 atctttttga atgcttcttt cgtaatatga ttggtccctt tcgttcgatc ttccaatacg
    27421 tcaccatagt gataatgatc catgatatag ctcaaatgac gatctgattc acgatcacgt
    27481 ttgtaccgac gttgccataa cacttcaaag tctgctgtta ggcgaatcgt gatcacctga
    27541 tatccgtatc tatctgctaa ttcttggagt cgtgcttttt gcttatcact aaaaggatac
    27601 tcggacacaa tcactttctt tcctgcttcc atatacaaat caagtgcttt gtaatagaaa
    27661 ggataaactt tcgtcctttc ttgttccgcc cgttcttcca aattgttaaa cccgatggat
    27721 tctgcatagt attccttgat ttcatcgagc gtgatggcat acatatctgg aaatcgttcc
    27781 cgtagtttat tcataagata tgtttttcct gtaccaggac taccagccag aagaatgaaa
    27841 tactttttca tctcatttat ttcctaagtt aattggactt tccaagttca taaacagtcc
    27901 aatcttatgc ttgacaattt cttttactgc ttcatttgct ggaatcacat tatggcgaag
    27961 agacttgttg atttctgttt cactaaaggt ttctttagct actttcgtcc agttgactaa
    28021 aagctccgtt ccaacattga atttgcggat cccgttattg atcaattcag gataatcttc
    28081 ttctttcaca cctgttcctc catggataac tagggggaca tcaactactg catcgatttc
    28141 tttcagcaag ggaatgttta cttctgtttt tgatttgaat tgtccatgat tcgtaccgat
    28201 agcaatagct aatgcatcca cgcctgtagc ttctacaaat ttcactgcgt cttccggtct
    28261 tgtatatacc ttgtcatttt cagcgacatg gattccttct tctgtaccgc cgattgttcc
    28321 aagttctcct tctacggaaa caccgtgcgc atgggcatat tctactactg cttttgtctt
    28381 caggatattt tctttgaaag gcagatggga cccatcatac atgacagaag tatatccgct
    28441 gtcgattgct tctttgatat catcaaagtc tcgggcgtgg tctaagtgca agactgcatc
    28501 aaccatttct tcttctgcaa tagctgtcat tacattgact aacacatgca tgccgatata
    28561 tttagcggta tcgacacttg tttgaataat gatcggcgaa cccatttctt ttgctgcgcc
    28621 tagcatagca ggaagcattt ctaggttatg cgtattaaaa gctccaactg taaaattcaa
    28681 ctcttctgct gtttttgtta cttctttcaa tgttgtatac atcttttatt gccaccttat
    28741 ctttttattt gtttcctttg atcgttactt gttttgcaat ttgtcgcatc tcatccattt
    28801 tgcccatgta gtaatcgatc ccttgatcat caccagcacg agcagcatct gcacgcttct
    28861 ggttatagag gctcatcagt tttttatacc catctccgat cgtctgttta ttttccaaac
    28921 tcttttctaa atattggatg gcttgatcgt tcacatacaa ttcagaaaat ttaagtccaa
    28981 cttgttcata aagtttcgct aatttatgct tatcttgggt agtggaaatc tcttgtttca
    29041 attgtgtaat ctctgattga atcttttcgg tcgcttctgg cgatagggct ttttcttctt
    29101 ttgccagaac ttttttcggt tctttttctt ttttcttaaa aaatccaaac atcattttcc
    29161 ctactttcaa ttaaaatgct ttcaaaactg ggcttaagat ccaggcatat aagagtgctg
    29221 cggctactgt atctacatcg gttgctgtag cattgttgaa tcccatcgaa ttaaagatcg
    29281 ttactagcaa agcgggcaat aatgtaataa agaaaccatg agcaataccg ccaatgatcg
    29341 ctcctcggcg tccgccaact gcgttgccaa atatccctgc tgtccctccg gcaaagaaat
    29401 tggttagcat accaggtaaa atcatcgcta agccgaacat gggcaaagta aacatggcaa
    29461 taatcgtacc aatcgttgtc gtaatgaaac ctaaaatgac agcatttggg ctataaggaa
    29521 agaaaactgg acaatctaag gcagggatcg catcaggaac taatttcata gctatccctc
    29581 gaaatgccgg cacgatttca cctaacagta gacgtacacc agctagcaat acatacacac
    29641 ccactacgaa ttggatggct tgtaaaaagg caaacatgat atagttttga tctcctgata
    29701 attcagaagc gaatggttcg cctgcaaata aaacagtgat gatatatagc gggaccatca
    29761 cgaccatgac agacaaataa gtatcttgca gaaattcgaa atgtgtcggc aacttgatat
    29821 cttcgatcga ctttttattt tcgcctttct caccaaaaag cttagcaaca ccggcttcaa
    29881 ataagtaccc gatcgtacaa aaatgaccta aagcaatatc atttgatcca gtgattttac
    29941 gaatgatcgg ttgcgctact gccggcatag caatggcgaa acaagctccg ataaaaccac
    30001 cgaccaagat caatacgatc cctcgcagac cggcaaaata gccaaagact gtcgtcatcg
    30061 ttgccatcca taaaattgct tgaccagtta aaaaaatgta cttccatttt gtgaaacgag
    30121 ccaaaataat attgaataca aaaatggcta agaacgtcag tgcaatatct ctacctaaac
    30181 ccaaatcatt cattgcttga ccattgatag attcaataga agggatgatc ccttgcatat
    30241 gaaatccttc agtaaagatt ttcccgaaat aagttaaact tccaacgatg atgctcgaac
    30301 cggcactcaa cacttgaaat cctaggagtg tctttaacgt gccagagatg acttgtcctg
    30361 ctgatttctt ctgcagtagt agacccaaca tagcaatcaa tgcaatggtg atcgatgcct
    30421 gagtcaaaat attttcgatg ataaaattga taacgcccat
//
LOCUS       DACEKG010000032        29697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEKG010000032 DACEKG010000000
VERSION     DACEKG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.032"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(149..619)
                     /locus_tag="IW664_001344"
     CDS             complement(149..619)
                     /locus_tag="IW664_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0848714.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            949..2589
                     /locus_tag="IW664_001345"
     CDS             949..2589
                     /locus_tag="IW664_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0848715.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2743..4065
                     /locus_tag="IW664_001346"
     CDS             2743..4065
                     /locus_tag="IW664_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0848716.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4133..4564)
                     /locus_tag="IW664_001347"
     CDS             complement(4133..4564)
                     /locus_tag="IW664_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0848717.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4629..5465)
                     /locus_tag="IW664_001348"
     CDS             complement(4629..5465)
                     /locus_tag="IW664_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0848718.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6034..6339)
                     /locus_tag="IW664_001349"
     CDS             complement(6034..6339)
                     /locus_tag="IW664_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0848719.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6364..6795)
                     /gene="ruvX"
                     /locus_tag="IW664_001350"
     CDS             complement(6364..6795)
                     /gene="ruvX"
                     /locus_tag="IW664_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0848720.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6792..7058)
                     /locus_tag="IW664_001351"
     CDS             complement(6792..7058)
                     /locus_tag="IW664_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0848721.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7313..8038)
                     /locus_tag="IW664_001352"
     CDS             complement(7313..8038)
                     /locus_tag="IW664_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0848722.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8399..8469)
                     /locus_tag="IW664_001353"
     tRNA            complement(8399..8469)
                     /locus_tag="IW664_001353"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8435..8437),aa:Gly,seq:ccc)
     gene            8586..9338
                     /locus_tag="IW664_001354"
     CDS             8586..9338
                     /locus_tag="IW664_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0848723.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9354..9833
                     /locus_tag="IW664_001355"
     CDS             9354..9833
                     /locus_tag="IW664_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0848724.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10084..11664)
                     /locus_tag="IW664_001356"
     CDS             complement(10084..11664)
                     /locus_tag="IW664_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0848725.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11657..12400)
                     /locus_tag="IW664_001357"
     CDS             complement(11657..12400)
                     /locus_tag="IW664_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848726.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12576..13352)
                     /locus_tag="IW664_001358"
     CDS             complement(12576..13352)
                     /locus_tag="IW664_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0848727.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13600..14436)
                     /locus_tag="IW664_001359"
     CDS             complement(13600..14436)
                     /locus_tag="IW664_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0848728.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(14447..15376)
                     /locus_tag="IW664_001360"
     CDS             complement(14447..15376)
                     /locus_tag="IW664_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0848729.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15568..15921
                     /locus_tag="IW664_001361"
     CDS             15568..15921
                     /locus_tag="IW664_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0848730.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(15995..16651)
                     /locus_tag="IW664_001362"
     CDS             complement(15995..16651)
                     /locus_tag="IW664_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0848731.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            16877..18811
                     /locus_tag="IW664_001363"
     CDS             16877..18811
                     /locus_tag="IW664_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0848732.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(18897..20120)
                     /locus_tag="IW664_001364"
     CDS             complement(18897..20120)
                     /locus_tag="IW664_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0848733.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20114..22012)
                     /locus_tag="IW664_001365"
     CDS             complement(20114..22012)
                     /locus_tag="IW664_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0848734.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(22006..23124)
                     /locus_tag="IW664_001366"
     CDS             complement(22006..23124)
                     /locus_tag="IW664_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0848735.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23207..24040)
                     /locus_tag="IW664_001367"
     CDS             complement(23207..24040)
                     /locus_tag="IW664_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0848736.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(24033..24950)
                     /locus_tag="IW664_001368"
     CDS             complement(24033..24950)
                     /locus_tag="IW664_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0848737.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24973..25152)
                     /locus_tag="IW664_001369"
     CDS             complement(24973..25152)
                     /locus_tag="IW664_001369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848738.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(25177..26301)
                     /locus_tag="IW664_001370"
     CDS             complement(25177..26301)
                     /locus_tag="IW664_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0848739.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            26484..27392
                     /locus_tag="IW664_001371"
     CDS             26484..27392
                     /locus_tag="IW664_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0848740.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            27533..29146
                     /locus_tag="IW664_001372"
     CDS             27533..29146
                     /locus_tag="IW664_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0848741.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9180 a         5814 c         5071 g         9632 t
ORIGIN      
        1 tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
       61 ttattacttg atgcagggca ggtttttcac gacctctgat tcacggtgtt tctttttcat
      121 caacattttt acgaaatact gtggaagact attgaaataa gtgtggattg tttctttgtg
      181 tttcaatcgt ttcttgccat tcttcattta attttggatt accgttttct tccttgaact
      241 cataaggtcc taattcaaaa atcccagaca gtatttgatt cacgatttca tatagttcag
      301 tgtgatcaga cacatgattc ttgaagatca tctcattatc accacgtttg aacctcacat
      361 tcgggctctc atgatgaacc tctatgcatt tttctccttt ttttaactga cggacttctg
      421 aaaacatctc ataaatatct tctccagtaa aaccttcagc aagcgccaga ctgtccacaa
      481 gaccgcctac tgtcagttca gagattattg cttgattgga atctaatttt aatgtatagc
      541 ttgtcgcttc tactacgcca catgcattgc aaccgccttc tgatagttcg ctttttctaa
      601 tcaattgaac ttctttcatt ttctcttcct cctcttaatt ttctacttat gtatttttat
      661 cacctgacat aggtgactcc tatcaacgct atcatgtgct tttttcctca gaaatcctat
      721 ttatccattc catctatccc ttattcttct acttttcttt ctctagcata taagagatac
      781 gcatagtatt caagcaagga ttgtcgatat aaaaagaaca aatactgcta ttttgatttc
      841 ttttggctat attacatata aataaaattt atcatacctg ctctttttga ctatatcatc
      901 atctgacttc tatacttcgt ttgtattaaa acttaggagg aatcattcat gcttacaggg
      961 ttaaacttat taaataatcc ttttctaaat aaaggaacag cttttacaaa agaagaacgg
     1021 gcaaaatatg gaatcactgg tatgcttcca agtactgtcc aaacactgga acaacaatct
     1081 gttcaagctt atggtcaata cttaagcaaa caaacagatt tggaaaaacg tatcttctta
     1141 atgaatctat tcaatacgaa tcggacattg ttttataaat tgatgggcga acatttagtt
     1201 gaattcatgc ctgtcgttta tgatcctgtc gttgcggatt caatcgaaca atacaacgaa
     1261 attttcttga aaccgcaaga tgctgcattt ctttcaatcg acgaaccaga aaacatcaaa
     1321 gcaagtttga agaacgcggc agatggacga gacattcgtt tgattgttgt gactgatgca
     1381 gaaggaatct taggtatggg cgactggggt gtgaacggtg ttgacatcgc tatcggcaaa
     1441 ttgatggtct atactgctgc tgctggtatt aatccagcac aagttttgcc tgtatcgatc
     1501 gacgctggta ctaataatga agaattattg aataatccct tatacttagg caatcgtcat
     1561 gctcgtgtag aaggcgaaac ttactatgaa ttcatcgatc aattcgttca atcagctacc
     1621 gaattattcc cagaattgtt attgcattgg gaagattttg gacgaggaaa tgctgcagcg
     1681 atcttagaaa aatacgaaga tcaaatcact actttcaacg acgatatcca aggtacagga
     1741 attgttgtcc tagcaggtgt attaggcgga ttgaatatat ccggtgaaca attgaaagat
     1801 caaataattt tgacatttgg tgctggtact gctggtgtgg gtatcgcaaa catcctttta
     1861 gatgagatga tccgtcaagg tgtacctgaa gaagaagcac gccaacattt ctatcaagta
     1921 gataaacaag gtttattgtt tgaagataca gaaggattga ctcctggtca aatcccattc
     1981 gctcgcaaac gctcagattt cactaatgct gatgaattga caaacttaga agcagttgta
     2041 aaagcgatcc atccgacaat catgattggt acttctaccc aacctggagc attcactgaa
     2101 gaaatcgtga aagaaatggc tgctcataca ccacgtccga tcatcatgcc tttgtctaat
     2161 ccaacaaaat tggcagaagc aaaagcaaaa gatttaatag aatggacgga cggaaaagca
     2221 ttagttggaa caggtatccc tgctgctgat gttgaatata atggtgtaac ttaccaaatc
     2281 ggtcaagcaa ataatgcttt gatgtaccca ggtttaggac ttggtttgat tgcttcaaca
     2341 gctacacgag taaatgctga aatcatttct caagctagtc gagcattagg cggaatcgtt
     2401 gatgtaacaa aaccaggagc agctatcttg cctccagtag caaaaatcac tgaattttct
     2461 caaacgattg ctgaaactgt cgcaaaatca gttgtcgcac aaaacttgaa tcgtgaagaa
     2521 atcacggata tcaaagaagc agttgaatct gctaaatggg ttcctgaata taaatcttta
     2581 gaagattaag atcgttatag taaaagaaca tacagaaact ttgtatgacg tctaaaaaga
     2641 aaaaagcgac tgcggcaaaa cagcggccag ccaactcctg ctcttttgtc gcttttcttt
     2701 ggcttttttc atatgataca gaataagaaa ggatctgtaa aaatggcaca aaaaacaaca
     2761 actgcaactg cagttgagga aaagaaaaaa gaaggttttt ggtccacaaa aatcaccggc
     2821 gtaagtttgc cactttattt gatcatggtc cttgtcctga tcattgcaat ggctttaggg
     2881 aaattaccga cgaatatgat tggaccaatc gcagttttgg ttattttcgg taatttattt
     2941 cattttatcg ggacaaaaat ccctatcgtc aaaagttatt tgggtggtgg ttccgttttt
     3001 gcgatcttcg cttctgctgc gattgctaca tttgggatct tacctgaata tgtagtgaaa
     3061 tccacagaaa actttgtcag caatatgggc tttttggact tttatattgc tgccttgatt
     3121 actggtagca tccttggtat gaaccgtaat ctattgatga aagcttctgt tcgttttatt
     3181 cccgttgcac ttatttcaat ggtcgtctcc ttttttgccg taggactcgt gggcatgctt
     3241 atcggaaatg gttttgcaaa ttccgtttta tacatctcat tacctgcgat ggctggtggt
     3301 gttggagccg gagccgttcc tttatcaacg atttatgcaa atgttttagg tacggatgct
     3361 tcttctatca tttcccgcct gattcctgcg acagcaatga caaatgtctt agctatcatc
     3421 ggtgcagcat tagttgctcg tgctggacaa tcaatgccaa aattcaacgg caatggtaaa
     3481 ttaatggaaa aaggcgatct tggcgatgga aaacctcgaa ctgtgaaacc ttccattcca
     3541 cagttaggtg ttggtttgat ggtatcattg actttcttca tcttaggaca aattggtaac
     3601 tattttgttc ctaaagtcca tgcttacgca tttatgatta ttatcgttgt catctgtaaa
     3661 atcgccaacc tattaccaga gtattatgaa gatgcagcta tcatgttcaa taacttgatt
     3721 gtcaaaaact tgacagcagc tgttcttgcc ggtatcggta ttgcgttgct gaatctgaat
     3781 gtattagctt ctgctttgac atggcaattt gttgtcttat gtctgacaag tgttatcgcg
     3841 atttcagttg tttcaggatt tgtcggtcgt ttgtttggtt tatatccaat tgaatcaact
     3901 attactgctg gcttatgcaa caactcgatg ggcggaacag gaaatgtagc cgtcttatct
     3961 gcagctaatc ggatggaact tattgctttt gctcaaatgg gcaatcgact tggtggtgcc
     4021 atcgttttga tcatctctgg tttcttgatg caattacttt cttagtcaaa aaaacaaaaa
     4081 tacctgatca tcaagtatcg gcttggtgat cgggtatttt tttgataaag atttacagga
     4141 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgtgttca taaaattggt
     4201 gtgcttctac tctgtattct gcagaattaa gccttattgt cgttattcct cgttttttcg
     4261 cttcttctac gccagtcatc aaagcctttc cgatgcctct tttttcagca tatttagaca
     4321 cagcaagtgc cataatattg aacattggat cgctaaaaat cgattcatag gactccgctt
     4381 gtacatagcc agctgctttt ttagaagtag gatcttcata aaccaagaaa aaatttttct
     4441 ctggctcctt cagcagtttc ttcatttgtc tcaccgtaac ggttaatggt atatcatacc
     4501 ctaattgatc cgcattgatt tcacgtagtg cttctgcatc ttctaaacgc atttcccgta
     4561 acatccaaat caccagccaa tttctgattt tttaaaatga atttttttat catagtatac
     4621 tctttttttc atttagaaca atatgtttca gtgattttga cttgcgttag tctagattct
     4681 cctaatgttt gtaagatgac aaaggcattg taacaattga ccaaaagaac cacgaggaca
     4741 attacttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
     4801 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
     4861 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
     4921 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
     4981 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
     5041 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
     5101 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
     5161 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
     5221 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
     5281 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
     5341 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
     5401 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
     5461 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
     5521 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
     5581 agtgctaaac gaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
     5641 aacaaaaata gctcctcata attttcgttt ggttgaactt attaccgcag atgcaagctt
     5701 ttgaacaccg tttaagaaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
     5761 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttgg
     5821 caggagaaca ccgtttaagg aagagactgt gaagtcaacg ttttgacctg attattagag
     5881 aaacaaatgg caaaatagtt cttcatattt cgccgttttt cgagcgaatg ccgaggggca
     5941 gctgattgaa catcgtttat ttggaaaaag ggattgtgac aagacgtttg tcacaatccc
     6001 tttttttata tcttattaat tcattctttt gttctattct tcttcttccg ccatgaatgt
     6061 attgaatact tcttcaatca tgtcccattc tgcgtctgtt tcgatttgtt ctaactcgcc
     6121 ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt aattctacat cttcatcttc
     6181 tgaaacacca gcaggatata aaagaacata gttacgtcca aattcttctt gtccgtcaac
     6241 tgttagtagg atttcatata atgtttcatt tccttggtca tccaccaatg tgatgtgttc
     6301 gtgtccttca tggtcgtggg aatggttatg ttctgtcata agattacctc ttttctttta
     6361 ttattaaaat aattgcttac ctgagccgtc taaataattt tgtaagatca tcacagctgc
     6421 taatttatcg atgacttttt ttcgtttggc tcttgaagta tttgcctgct ctactagcat
     6481 acgttctgcc tgaacagtgg ttaatcgttc atcttgataa tcaactggca aggagaatag
     6541 ttctttgatc ttttctccgt atgacatgga agcttctgcc cttggtccga tggaattgtt
     6601 catattttta ggtaatccaa ctacaaaccg gtctacttca tattgtgcta ctaattcctt
     6661 caaccgatca aagccaaact cttcttctgc ttcattgatc cgaatgatct ctactccttg
     6721 agctgtccag ccaaaggggt cactgatagc aacacctact gtcttagaac cgacatccaa
     6781 gcccattact ctcataaatt cactccatga tttgagaggt agtccttgac aattacttcc
     6841 atgatctcat cgcgttcgtg acggcgaatc aaatttcgag catcacgata acgaggaatg
     6901 taggccgggt ctccagaaag caagtagccg acaatctgat tgattggatt gtagcctttt
     6961 tcttccaaag cccgataaac aatcgctaat gtttcgctca cttctttttt gcgactatcg
     7021 tcaaaaccaa aacgtacggt ttcatctgta aaacccatga ttctacacct ctttcccgca
     7081 aggtaattcc agcttacctt ctacaacctt attttactcg aagaaaggga aaactacaaa
     7141 caaatattcc aaacttccga ataaattaag aaacactatt tgtatgtttt tttccttttt
     7201 tctgtagctt tatcaaaagt actcttttca taaagtatac gctatatttc tttgaatgta
     7261 tgtttttaca caattttttc ataatattca taaaaaattc gaatagctgc tactaatttt
     7321 ttgtttttgt tgcttgtcct gtctcataat tgatcgtgta gccatcttga acattgtaga
     7381 taaagacatt gaattcaatt tttttgtctt caatgctttc tgcttccaat tgcactcccc
     7441 tggctaccag ttcattcccc tcaaaatgag gagtgacacg gtagcgtaca tgatgatttg
     7501 tttctttgat atatgccgca atatcatttt cgtgagcaag catgtatgga gcattcaacg
     7561 aacgtgtccc tgtcatcaag tttttaatgt tgttattttg accagtcagc tggaaaccaa
     7621 ttaaatggct gcgattatat aaccattgcc cgtcacttaa tttcttttgt ttccaacctg
     7681 tcgggtcaat atacaaaggc tctctctttt cagtagggaa caaatctttt ccaagcatag
     7741 cattggcttg tcctactctg ttcaatttat caatatttga atagctttcc catgttcctt
     7801 tggaaaggct aagatcctct tttgtaaatt ccggctgatt cccattgatt tctatttctt
     7861 gatgttttcc atcatattct ggcacatccg ctacttgaga agcttgtggt gtggaatcaa
     7921 aaatagttgt aatctgctga aagatatctg aatctttttg tgtggtggaa acaccgaatg
     7981 cccctgcaat cacagcagcg acgatcccca cgatcgcctt ggttgttttt ttgctcattt
     8041 ctttactcct ttactaacag ctccctaaat cttaacatat gttcccctat aaataaatac
     8101 tagaaagaaa tagaaacagc aaaaaataaa acctgtttta agatggctta aaaatacaaa
     8161 aaagagacgg attcctcgtc tctaatcagt caccactcta tctgtaaggt atttattatt
     8221 attcaaaaat tttcttcata ctcaagacaa tacactgatt tcacaaccgc ttggtaaacg
     8281 gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa gttaaaaaca taagaagttg
     8341 tttttgcttg gctgttctta ttacttgttg cagagcagct ttttcacaac ctattttaag
     8401 cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag ctgggatttt accactaaac
     8461 tacacccgca atacaaaaac agtatacgcc tcttttattc cttcctcaag tttttttatt
     8521 ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa tcaatcaatt attggagaag
     8581 atgctatgac gggaaaagaa ttagatctgt tgctggacac ttgtttactc gctgggaaaa
     8641 tcatgatgga aagcaatgcg gagatgtatc gggtagaaga tacgatgagt cgaattgctt
     8701 tagcttctgg aaatttccgt ttggtcagct atgtcactca aaccggtctc tttattggtc
     8761 ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa ccgttcgatt aatttagaaa
     8821 aagtagtaaa agtaaatgat ctttcacgta aatatgttgc ttcagagcta acattagaag
     8881 aactttactg cgaactgcaa gcaatcgacc gagaccgttc tttctttcct gtctggttgc
     8941 aaatcgttag cgcggcagtg atcagtggaa caattatggt gttatttggc ggaaagatga
     9001 ccgatttacc tgtgacttta gtcattggag gattgggata cgcagcttat ctatatagtt
     9061 tgaaattttt tagaatcaaa tttttatcgg aatttttatc ttctttattg attggcatag
     9121 ccgctatcta cagcgtacgt tttggcttag gaagcaatca ggatctaatc attatcggat
     9181 gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc tatccgtgat ttgctagctg
     9241 gacattatgt ctcaggagtt tctcgcggaa cagaagcgat gatgactgct acgatgatcg
     9301 gttttgcgat tgcttttatt ttccagcttt tttattagga aaggaacaca agtatgggga
     9361 cgatatttat tcagttttct tttagttttt tagcgactgc ggcatttgcc gttattacaa
     9421 atgtacctag gcgttcattg atttgttgtg gtctttctgg tgcttttggc tggatgatct
     9481 actggatctg tgttcaaata ggcggatctg ctgcttttgg ttcattgctt ggttctttag
     9541 gagtagcatt catcagcgat cttttctcta aacgtttgaa aatgcccgta acgattttca
     9601 atattcccgg gatggttcct ctagtccctg gtggtttagc ttatcaagcg gttcgaaatc
     9661 tcgttactga atcctatcga gaagcggctc attatgcggt ccaagcaatt atgattgctg
     9721 gagcaatcgc tttaggtctt gtattatcag aagtctttaa ccataacatc cgtaatttca
     9781 aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg gctttttaaa taaacgaagt
     9841 gtaacaaaga aaaggttgtg aaagagacgt tcagccttga gcgatgacaa aatatctcac
     9901 aaacattgcg acaagtaacc gcaggagcaa taagaagtaa ttatgagtat taaaaaaagt
     9961 tttgtaaaaa tagcttctca catttttaca taaaattgac ttaatactga gaaactgact
    10021 tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg atttttgtca cggcccctct
    10081 tctttattca aattttttcc aaaacgttcg ttgataataa acgatccata gagcggaaag
    10141 tattagaact aaaccgatca caggaatatt cactaaccaa aatggataaa acaaagccaa
    10201 cattccgtat aagacgataa gaataatact gatgatcaat gaacccatca tagttgcgac
    10261 taaacccaaa cttcctgaac ctctgttaaa taattggtta atattggtcc agttcagcag
    10321 cagcaaccga taatctctag cgaaataacg tagtgacaat aagtagcagc cgaataaaga
    10381 accaaatagc aagctaggga taaaaaacaa aggcagctga aaagacagtc cgcttaataa
    10441 tgcgatccca ccagttaaaa aactctgcaa tatccatccg aatctgaact tttcttttaa
    10501 atattggttc atagagattg gcaaagaacg gataaacaga aaattttctt gatccaaaga
    10561 aataagattg ctgataaaag aagtctgatt aactgttaag aaagccaatg cgatccctac
    10621 caggaaaaca acaccgatta acttattatc taaatcagtt aattttattt cgcctcctag
    10681 ggcaaaagca agaataaaaa tgattggagt taacaaggaa gaagaaagaa cttgcataat
    10741 caaattaggt tcttttacta attgagcatt gtaactaaat aaaagctgat gcacattttg
    10801 gttcttttta tgggttcttt ttattttgaa ctcactgttt gaatcagtca gttgctctgt
    10861 aagctttggt actatcagct gttttacagc aaggaaaaat cccagagccg tgatagctag
    10921 tattccccaa cccaataaac cttgttgaga aagtggctga ctggcagcat catagaatgg
    10981 caataatatc gaaatcggat gccgatctgt taactcattt tcaatagtcc ccgtttgatg
    11041 attcatccac atgattccag caaatgcgat cagcatcgag ctgccaagca agatactaga
    11101 aactgttttt ctatgttttt taaacactgc tgttcgagcc aaaccaaaaa caatcaaact
    11161 agccagacaa aaaagaataa tcaaaaatag agaaaacaaa aggattgaga taatgatagt
    11221 aagcaaaatc cctagatcag ctcgccatcc ggttaaaata aacatgacta gaagcggaaa
    11281 aacaaaagga atgatggtca aagcaactac caaaatcttt gctgaaaaaa cctgttcttg
    11341 tccaaaaggc agaggcaaaa atacttgcag gtcatttcct tcaaaaaaga tattataaat
    11401 gactgatata ctttgagtta aacctaacat accaaaaagt gccacgtaat aagtaaagaa
    11461 acctggcaat ttaacaaagt tcatagcaaa catagcaaat ccgtaaatca gtaaaaaaat
    11521 cagccctgaa aaaagatttt gagacaaaag atagatagtt aatcgctttc ccttttttcc
    11581 tttctctctt gctttattcg tgagctgtgg attaacatat aaaagattaa ctcgaattaa
    11641 ttctaataat tgtcgcttat acatacggtc catctcctgt cgcttgcctt ccagccattt
    11701 tcaaataaat cttttctagc gtttcatcag gtgagccatt caataattca ctaactgccc
    11761 cctgataaat caattcacct ttcttcaaaa tagccagttt atcgcataac tgttgtgcgg
    11821 tatccaatac atgtgtagaa aaaatgactg tttttccttt tgcggcatga gccttcatca
    11881 tttctttcaa atcaaatgct gcttgaggat caagtccttg caaaggttca tccaataccc
    11941 aaatatctgg atctggcagc aacgtgccga tcaaaatcgt tttttgacgc attccatgtg
    12001 aaaaacttgc gattgtttca ttttgatgac tatacatatc aaaaagcgct gtcaattctg
    12061 ctaaacgctt ttcttttttt tgtgtgtcta attcataggc agctgaaatc aagtcccaat
    12121 attctccagc cgtcaattgt aaaaaaatat ctggggaatc aggcacataa ccgatttttt
    12181 gtttgatact caatcgattt tccgtcaatt tcattccatc tactaaaatc gttcccgatg
    12241 atggttcaat aatactaact aaacttttaa tcgtagttga ttttccggca ccattatgtc
    12301 ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga caagtctttt aatgcttgtt
    12361 ttttcccgta acttttactt acattgatga attcaatcat ttctgtactc cttttgtttg
    12421 ttattacttt ttattgtacc cttcgttatt ggtggagtaa atgtcaaaaa gagtgcataa
    12481 caaaaaagga tagaaacgac taggctgcta cttgggttgt tctcttctac aggaaaacac
    12541 tcttctaaca ggtaagatat gagtctttgt tttctttaaa atgattcatt ttctatcttt
    12601 tcttgcataa aattctttag tttatcaata cttctctttc ccatctctag ttgttctcca
    12661 tttgcaaatg ttactatttc gtttactctg ctaatagatg taatgttttc agtattaatg
    12721 atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt atatttttta
    12781 attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt atttttataa
    12841 cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc gctattacca
    12901 taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt ttctttttct
    12961 ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc ttttatcaaa
    13021 taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg atgagacgtt
    13081 agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag ttcgataccg
    13141 ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata gatttcatta
    13201 tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc aaatttccaa
    13261 ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc aaaaaccaaa
    13321 ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg atattttact
    13381 atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg taattctctt
    13441 cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa
    13501 aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc
    13561 gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata
    13621 ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg
    13681 cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc attcatcatt
    13741 tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac
    13801 acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg
    13861 gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat
    13921 tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata
    13981 cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt
    14041 atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg
    14101 gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt
    14161 ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga
    14221 caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca
    14281 caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta
    14341 gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa
    14401 ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg
    14461 tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa
    14521 aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg
    14581 cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt
    14641 ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg
    14701 ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta
    14761 atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca
    14821 catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt
    14881 catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag
    14941 cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta
    15001 tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca
    15061 cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc
    15121 cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa
    15181 atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca
    15241 cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga
    15301 aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg
    15361 gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat
    15421 acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta
    15481 ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc
    15541 acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa
    15601 gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt
    15661 tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt
    15721 catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca
    15781 gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt
    15841 aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa
    15901 gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa
    15961 gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg
    16021 catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat
    16081 tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt
    16141 tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta
    16201 aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa
    16261 cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga
    16321 tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc aagttcccta
    16381 caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca
    16441 taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat
    16501 ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt
    16561 ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag
    16621 ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac
    16681 atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat
    16741 attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat
    16801 ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag
    16861 tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc attttggtgc
    16921 agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt
    16981 tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc
    17041 tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac
    17101 aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag
    17161 acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac
    17221 acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg
    17281 aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc
    17341 ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt
    17401 agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa cgaaccattt
    17461 agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt
    17521 gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag
    17581 cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga
    17641 acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga
    17701 agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag
    17761 aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa
    17821 ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc
    17881 tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga
    17941 agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat
    18001 tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta
    18061 ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga
    18121 tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc
    18181 tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc
    18241 tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca
    18301 tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact
    18361 gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact
    18421 ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca
    18481 ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc
    18541 cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca
    18601 acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt
    18661 ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa
    18721 ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact
    18781 aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg
    18841 ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac
    18901 caataagaag accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc
    18961 caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa
    19021 aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa
    19081 acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct
    19141 aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca
    19201 tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat aaaataattg
    19261 gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca
    19321 aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga
    19381 tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt
    19441 gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt
    19501 ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa
    19561 caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata
    19621 actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc
    19681 ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa
    19741 ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct
    19801 gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta
    19861 attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt
    19921 ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc
    19981 gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa
    20041 cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt
    20101 tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta
    20161 gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat
    20221 cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac
    20281 cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca
    20341 taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc
    20401 cagcttcact caccctatag ttttccttgt cttcttctat gattattcct tctttctcag
    20461 gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt
    20521 tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta
    20581 aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat
    20641 ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat
    20701 atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat
    20761 tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat
    20821 tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat
    20881 tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca
    20941 taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa
    21001 tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag
    21061 ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc
    21121 atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg
    21181 cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa
    21241 aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag
    21301 ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac
    21361 cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat
    21421 accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt
    21481 ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc
    21541 aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata atctggcttt
    21601 tttctcctgt atctaatttt ttccataata attgttcatg atgatataac gctaataaca
    21661 ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat
    21721 gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta
    21781 attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa
    21841 ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat
    21901 aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc
    21961 ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc aaatagctaa
    22021 ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg
    22081 cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc
    22141 atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc
    22201 atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc
    22261 agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg
    22321 aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc
    22381 tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc
    22441 atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata
    22501 tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag
    22561 atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt
    22621 ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc
    22681 aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag
    22741 taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg
    22801 tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta
    22861 cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt
    22921 tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat
    22981 tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt
    23041 ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga
    23101 ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg
    23161 gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag
    23221 ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag accattggga
    23281 tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag
    23341 aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga
    23401 ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa
    23461 tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat
    23521 cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa
    23581 acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc
    23641 ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag
    23701 atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat
    23761 aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca ataacaatcg
    23821 aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata
    23881 gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg
    23941 aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga
    24001 cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta
    24061 aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct
    24121 tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct
    24181 gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag
    24241 ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt
    24301 aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct
    24361 ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa
    24421 atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg
    24481 ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg
    24541 aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa
    24601 aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc
    24661 atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac
    24721 aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca
    24781 attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag
    24841 aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct
    24901 tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc
    24961 tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc
    25021 ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt
    25081 caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt
    25141 gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc
    25201 tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc
    25261 taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt
    25321 ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc
    25381 ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa
    25441 agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt
    25501 ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa
    25561 ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa
    25621 agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa
    25681 ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc
    25741 ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata
    25801 acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg
    25861 acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata
    25921 ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc
    25981 tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg
    26041 tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt
    26101 gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata
    26161 tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc
    26221 gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac
    26281 caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt atataaaacg
    26341 ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa aaaatatttt
    26401 acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa tagaaagctt
    26461 tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa tcctccaatc
    26521 ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct gctgctccct
    26581 attttcgact actaataaat gaaagcgcta atttatatgc tgaatttgca cataaatcta
    26641 tagaactaga taatccttcc cttttattca ttgataaaca gcaagaagtc caaatctttt
    26701 ctactcaaaa ggaaacacaa acttttatca ttatccagtt ttattttgat gagcaattat
    26761 tagcacatat tacgggacaa tctttttcta tagatcaaga aataacatct atcattactt
    26821 ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca tagtaagcca gctatttcct
    26881 atgatcagga aatcaattct tttttcatac tatgccaaca gatgagcatt catttgcttt
    26941 ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc ttccttacca aggcattatc
    27001 aacaggaact tgctttattg acaatacctt caaatgagaa agtcattcct aaaaaaaagc
    27061 atattcttta ttcagatgaa ttagttgatc caatcatacg atatttagaa gaacatcttt
    27121 cagaaaattt tttgcttgaa caattagccc aaattttttt tatcagtcca gccagcttaa
    27181 aaaaacattt tagaaaagtg aatggaaaaa gtgttattag ctattttaaa gaactaaaaa
    27241 tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac ctatacagaa attgcagata
    27301 tattaggatt tcattcgatc catcattttt cttcagcttt taaaaaatat acagggttat
    27361 ccccttcaaa gtattatcta tccttaaaat agaaaaaaat ttttaggttt tatccacaaa
    27421 ttatttctct atattttctg tctattcaaa ataaacaata attttccggc acaattattt
    27481 gctgtttttt accataacat agtacgatca acgtagaaaa ggagatgttt ttatgaaagt
    27541 tgttgtcatt ggcggtgtag ctggcggtcc ttcatttgcc actcgtttcc gtcgattgaa
    27601 tgaagcgcac gaaatcatta tctatgaacg cggagaaaat atttcttatg caagttgtgc
    27661 tttgccttat tatttaggag gtgtgatcac tgaccgtgac tcgctgatcg aacgtacacc
    27721 agaaatattg aaaacaaaaa acaacatcga tgtctttact aaacacgaag taacagcaat
    27781 cgatccttct actaagcgat taacagttaa agacctatcc acaaatgaag aaataaaaac
    27841 agattacgat aagttgatca tctcttctgg tgctagacca gattatccgg atattcccgg
    27901 agtttttgaa gcagaaaacg gctttgtcct ccgtagtgtg acggatgcgg atcgaatcaa
    27961 atcgttcctt gaagaaaaaa atccacaaca tgtcgtcatt cttggtgcag gtgttatggg
    28021 tctggaatta gctgagaatt tcaagcatcg cggcttagac gtgactttaa tcgatcaatt
    28081 gccacaagtc gctttccctt atgatccaga aattgctaat ttagtttatg acaaattgct
    28141 gaaagaagga ttagccgttc atttagaaac aagagttact gagatccgtg ataaaggtcg
    28201 agaaatagta ttatcagatg gctccgtcct ttctgctgat atgctaattt ttgctgttgg
    28261 tgtttctccg aagaatgaag tggtgaaagc aaccggcata caattatcag atacaggtca
    28321 gatcattgtt gatgaccagt tacaaaccaa tctgccggac atctatgcga ttggcgatat
    28381 tatcgaaacg actagtgtag tgactggtca gccgatccaa agtatgcttt ccagtgcggc
    28441 caatcgtcaa ggacatatgc tggcagatat tttaaatggt acgcctatgc gctatcgcgg
    28501 atatattggt gcaggtgtcg caaaaatctt tgatcataca gcaagttatg cgggaatgac
    28561 agaacatgaa ttaaaagcat caggcataac aaattataaa actgttttta tcactccctt
    28621 tgaccatgcc tatttctatc cagaagctac aagattaaat ctaaagctga tttttgatgc
    28681 agatagcggt cgtattttag gtggacaagc atttggagaa aaaggtgtcg ataaacggat
    28741 gggagaactt tctgtagcaa tcaccggaaa cttgacagtc tttgatttgc ctgatttgga
    28801 gttgccttac tctccaccgt attctactac ccgtgacccg ttgaatatag ctggttatgt
    28861 cgcaatcaat caaatgacga atatcgtaga aacgatcaaa gcaagtgata tacccgaaaa
    28921 cgatttgaaa gaagcgttct ttttagatat acgtgaacct aataaagcac cttctggcag
    28981 tatctcggca acaaaaaata ttccaatgaa tgagttacga gatcgaatca atgaaatccc
    29041 aaaagataaa aaagtttaca ttactttcag aagaggattg aatacttata cttctgcccg
    29101 aatcttggca ggtttgggta tcaaagcgat tttgattgaa gaataaaaaa acgaccgcgg
    29161 tgggtagccg cggtctagga gttattttta cttgaggagt aaaaatgaaa aagtatatag
    29221 ggttgttggt tggtatgtat atagaataga agataaatgt gaagaaattg tgcattattt
    29281 cttcacattt attaggttat agtttaagaa agtttaaaca ttgcgcaatt taattatttt
    29341 agctgtttct tttcataaaa aaacatgttg tgactctgag acaaatcata gtccatagtg
    29401 atgtctctag agtaactacg cctaatatta cgaggcatca tattaatcta tcagcactag
    29461 tacctaaact agcatccctt tcgaatattt tttcttatat aataaagttt gctttcactc
    29521 ctctaatctc accaaatcga ctggcttgcg ttgtgtgata ctaggaactg attggtttag
    29581 aacgactttt ctattgtaat catatttttc gccatcctcg ttgtcttcta atccttcaat
    29641 gaatgatccc taatgtgtgt attaaataat ttaatttttt tgttcctatt tatttaa
//
LOCUS       DACEKG010000033        28508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEKG010000033 DACEKG010000000
VERSION     DACEKG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.033"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..586)
                     /locus_tag="IW664_001373"
     CDS             complement(5..586)
                     /locus_tag="IW664_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0848742.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IW664_001374"
     CDS             complement(769..1395)
                     /locus_tag="IW664_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0848743.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IW664_001375"
     CDS             complement(1417..2748)
                     /locus_tag="IW664_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0848744.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IW664_001376"
     CDS             complement(2903..3304)
                     /locus_tag="IW664_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0848745.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IW664_001377"
     CDS             complement(3390..3596)
                     /locus_tag="IW664_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848746.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IW664_001378"
     CDS             complement(3691..5193)
                     /locus_tag="IW664_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0848747.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW664_001379"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW664_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0848748.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IW664_001380"
     CDS             complement(5764..6381)
                     /locus_tag="IW664_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848749.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IW664_001381"
     CDS             6602..7975
                     /locus_tag="IW664_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0848750.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IW664_001382"
     CDS             8050..9162
                     /locus_tag="IW664_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0848751.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTVHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IW664_001383"
     CDS             complement(9234..10871)
                     /locus_tag="IW664_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0848752.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IW664_001384"
     CDS             complement(11039..11647)
                     /locus_tag="IW664_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0848753.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW664_001385"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW664_001385"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0848754.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IW664_001386"
     CDS             complement(13201..13953)
                     /locus_tag="IW664_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848755.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IW664_001387"
     CDS             complement(14094..15044)
                     /locus_tag="IW664_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848756.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IW664_001388"
     CDS             complement(15045..15884)
                     /locus_tag="IW664_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0848757.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IW664_001389"
     CDS             complement(15881..16627)
                     /locus_tag="IW664_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848758.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IW664_001390"
     CDS             16865..18235
                     /locus_tag="IW664_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0848759.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IW664_001391"
     CDS             complement(18290..19186)
                     /locus_tag="IW664_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0848760.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IW664_001392"
     CDS             complement(19467..20144)
                     /locus_tag="IW664_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0848761.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IW664_001393"
     CDS             complement(20235..21140)
                     /locus_tag="IW664_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0848762.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IW664_001394"
     CDS             21315..22769
                     /locus_tag="IW664_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0848763.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            22785..23816
                     /locus_tag="IW664_001395"
     CDS             22785..23816
                     /locus_tag="IW664_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0848764.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IW664_001396"
     CDS             23823..25010
                     /locus_tag="IW664_001396"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0848765.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IW664_001397"
     CDS             25166..26548
                     /locus_tag="IW664_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0848766.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26520..26906
                     /locus_tag="IW664_001398"
     CDS             26520..26906
                     /locus_tag="IW664_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848767.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27106..27774)
                     /locus_tag="IW664_001399"
     CDS             complement(27106..27774)
                     /locus_tag="IW664_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0848768.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(27963..>28508)
                     /locus_tag="IW664_001400"
     CDS             complement(27963..>28508)
                     /locus_tag="IW664_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ0848769.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         7943 a         5821 c         5234 g         9510 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggatag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactgttc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt ttgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagatcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    26641 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    26701 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    26761 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    26821 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    26881 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    26941 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27001 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27061 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27121 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27181 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27241 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27301 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    27361 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    27421 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    27481 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    27541 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    27601 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    27661 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    27721 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    27781 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    27841 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    27901 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    27961 ttttacacaa ccgttacggt tgttttacga aattggacga gactttgcca gtttaatttc
    28021 tgttttatcc gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc
    28081 tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc
    28141 tccataagtg aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac
    28201 tccttcaatt tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac
    28261 gtacgacgat agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc
    28321 gctaatgcat tgggcttttc agagatacga taggataaaa tctcactatt gaacatatcc
    28381 ataaaaggat ctagatacaa tttcttttga cggataatgc ctttggcatc cacttcatgg
    28441 tatttaaatt cagttgtatc cgtcgtgatt ttctgatggc agatactggt gtagaaacga
    28501 cgatggat
//
LOCUS       DACEKG010000034        28474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEKG010000034 DACEKG010000000
VERSION     DACEKG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.034"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(168..863)
                     /locus_tag="IW664_001401"
     CDS             complement(168..863)
                     /locus_tag="IW664_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0848770.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(953..2053)
                     /gene="ychF"
                     /locus_tag="IW664_001402"
     CDS             complement(953..2053)
                     /gene="ychF"
                     /locus_tag="IW664_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0848771.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2062..2253)
                     /locus_tag="IW664_001403"
     CDS             complement(2062..2253)
                     /locus_tag="IW664_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0848772.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2274..3164)
                     /locus_tag="IW664_001404"
     CDS             complement(2274..3164)
                     /locus_tag="IW664_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0848773.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3151..3918)
                     /locus_tag="IW664_001405"
     CDS             complement(3151..3918)
                     /locus_tag="IW664_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0848774.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3930..4643)
                     /gene="rsmG"
                     /locus_tag="IW664_001406"
     CDS             complement(3930..4643)
                     /gene="rsmG"
                     /locus_tag="IW664_001406"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0848775.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4875..6776)
                     /gene="mnmG"
                     /locus_tag="IW664_001407"
     CDS             complement(4875..6776)
                     /gene="mnmG"
                     /locus_tag="IW664_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0848776.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6793..8190)
                     /gene="mnmE"
                     /locus_tag="IW664_001408"
     CDS             complement(6793..8190)
                     /gene="mnmE"
                     /locus_tag="IW664_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0848777.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8534..8872
                     /locus_tag="IW664_001409"
     CDS             8534..8872
                     /locus_tag="IW664_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848778.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9040..9798)
                     /locus_tag="IW664_001410"
     CDS             complement(9040..9798)
                     /locus_tag="IW664_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0848779.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9816..10634)
                     /locus_tag="IW664_001411"
     CDS             complement(9816..10634)
                     /locus_tag="IW664_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0848780.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10631..10987)
                     /gene="rnpA"
                     /locus_tag="IW664_001412"
     CDS             complement(10631..10987)
                     /gene="rnpA"
                     /locus_tag="IW664_001412"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0848781.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11105..11239)
                     /gene="rpmH"
                     /locus_tag="IW664_001413"
     CDS             complement(11105..11239)
                     /gene="rpmH"
                     /locus_tag="IW664_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0848782.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11762..13096
                     /gene="dnaA"
                     /locus_tag="IW664_001414"
     CDS             11762..13096
                     /gene="dnaA"
                     /locus_tag="IW664_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0848783.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13294..14424
                     /gene="dnaN"
                     /locus_tag="IW664_001415"
     CDS             13294..14424
                     /gene="dnaN"
                     /locus_tag="IW664_001415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0848784.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14653..14895
                     /gene="yaaA"
                     /locus_tag="IW664_001416"
     CDS             14653..14895
                     /gene="yaaA"
                     /locus_tag="IW664_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0848785.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14882..16006
                     /gene="recF"
                     /locus_tag="IW664_001417"
     CDS             14882..16006
                     /gene="recF"
                     /locus_tag="IW664_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0848786.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            16003..17949
                     /gene="gyrB"
                     /locus_tag="IW664_001418"
     CDS             16003..17949
                     /gene="gyrB"
                     /locus_tag="IW664_001418"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0848787.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17969..20440
                     /gene="gyrA"
                     /locus_tag="IW664_001419"
     CDS             17969..20440
                     /gene="gyrA"
                     /locus_tag="IW664_001419"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0848788.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20676..20975
                     /locus_tag="IW664_001420"
     CDS             20676..20975
                     /locus_tag="IW664_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0848789.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            21025..21537
                     /gene="ssb"
                     /locus_tag="IW664_001421"
     CDS             21025..21537
                     /gene="ssb"
                     /locus_tag="IW664_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0848790.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21563..21799
                     /gene="rpsR"
                     /locus_tag="IW664_001422"
     CDS             21563..21799
                     /gene="rpsR"
                     /locus_tag="IW664_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0848791.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21942..23915
                     /locus_tag="IW664_001423"
     CDS             21942..23915
                     /locus_tag="IW664_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0848792.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23921..24373
                     /gene="rplI"
                     /locus_tag="IW664_001424"
     CDS             23921..24373
                     /gene="rplI"
                     /locus_tag="IW664_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0848793.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24647..26014
                     /gene="dnaB"
                     /locus_tag="IW664_001425"
     CDS             24647..26014
                     /gene="dnaB"
                     /locus_tag="IW664_001425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0848794.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26794..28086
                     /locus_tag="IW664_001426"
     CDS             26794..28086
                     /locus_tag="IW664_001426"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0848795.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8673 a         5591 c         5655 g         8555 t
ORIGIN      
        1 attggactta ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg
       61 acaagacttt tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc
      121 tttactaccc ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg
      181 acggataaac ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca
      241 gttgccccga taatgataac tactactgga tctaatactg gattgataac tggtgtcaat
      301 agtgttgtcg cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat
      361 gtcttaccca ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg
      421 tatttgtaca tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa
      481 aagccataag ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt
      541 aatagaccaa gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt
      601 tttacttctg ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat
      661 tgacgggctg ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg
      721 gcttttactt tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa
      781 atgtactgtt gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt
      841 gctcgtaatt gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac
      901 ggtcgtcaca aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat
      961 taaagcggaa taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa
     1021 cacgtccagc ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga
     1081 cagtttctgc acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg
     1141 gtgctttgat tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt
     1201 aagtagctaa gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt
     1261 cgattcctaa agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt
     1321 cttctgcacg agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca
     1381 cttcttgtac ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta
     1441 cataaagaat tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt
     1501 cagtaaattc gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca
     1561 gtacagctag ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag
     1621 tatagcgttt gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa
     1681 tatcttctag tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc
     1741 gaacaacgtg acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac
     1801 cttctccttt acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg
     1861 gaacagtttt tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt
     1921 ctaccatccc tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg
     1981 cttttgtgat cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag
     2041 cagtcaatgc cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa
     2101 tttcttttca aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat
     2161 tttgatatct gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt
     2221 tttcatttca acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct
     2281 tcatcaaatt gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat
     2341 tcaatctcga tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta
     2401 tccatcaaac gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg
     2461 atttttttct tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg
     2521 cggacagtta aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt
     2581 tttaaaccta acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc
     2641 ttgacaagat ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt
     2701 cctaaacgac tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat
     2761 gcttctgctt cttctaacgg attcagatct tctcgctgca agttttccag cactgctact
     2821 tgcatcatcg cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct
     2881 agtttggacg ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa
     2941 cggcgtacga tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc
     3001 aaagatactt cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat
     3061 ggtagctgga caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga
     3121 aacagtgcat cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt
     3181 tgccaatgct tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt
     3241 tccatgacta ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt
     3301 ttcgcggaag tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc
     3361 atacattgtc agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa
     3421 acgaaccgta ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg
     3481 tatcaaaatc gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg
     3541 gcaatcgatc aaaatataat catactgact gcttacttct gctaaagatc cttttaatct
     3601 tgattctctt gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc
     3661 aggcacaata ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc
     3721 attgactaga acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact
     3781 ggtagcattt ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag
     3841 acaagcacct agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac
     3901 agatattatt tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg
     3961 ggctttcttg ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt
     4021 tgatcagaga cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa
     4081 gcaatcgctt gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa
     4141 aaatagcctt cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca
     4201 accgcacgag cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt
     4261 tctgcccggt catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag
     4321 aaggttattc gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt
     4381 tttaaaggta tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc
     4441 gtaaagtcta cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct
     4501 ttttcatccg taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa
     4561 taaatagcga attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat
     4621 tggcgaaatt cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac
     4681 agattctctc cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct
     4741 taaaaatttg aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa
     4801 tatacacaga agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta
     4861 tttattatta cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag
     4921 accattagga tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt
     4981 gtttctggtt ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct
     5041 gcgtagtcga tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt
     5101 tccatggctt ttttgatgta gccttcatat ttgatttgga cttctacttg ttcgatttct
     5161 tttcgtgtca attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca
     5221 ggacgtctta aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt
     5281 gctaaaaagt cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt
     5341 tcttgttcaa ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag
     5401 cctacacggt gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga
     5461 cggtattcag cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa
     5521 tcatcgatca tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc
     5581 tgaactttca aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa
     5641 ccgcttgtac catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat
     5701 gtcggacgga gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc
     5761 atttctactt tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt
     5821 aaagaagtag ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa
     5881 aataattgat gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa
     5941 tagcgagcgc ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct
     6001 cgaataattt ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt
     6061 ttataggcac tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc
     6121 tcttctgtaa cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg
     6181 aaccgttcga tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga
     6241 gagttattcg gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct
     6301 gttccggctg taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact
     6361 ccatgacaaa caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa
     6421 tttggttctt tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt
     6481 gctcgcaatg ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag
     6541 gttttatcga tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg
     6601 cctttagctg gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt
     6661 attgtcagta ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg
     6721 gcgtgtcctg cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt
     6781 ttgttcctcc ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc
     6841 ttgtacactg tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg
     6901 aacgagatcc acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga
     6961 agctttttct aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc
     7021 tgtttctccg ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt
     7081 tttagctacg gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg
     7141 ttccagctgt gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt
     7201 cgcttccaat aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa
     7261 gatcaagtct gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac
     7321 atcttctgtt tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt
     7381 gacatactct tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc
     7441 ttctcttaac aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc
     7501 cgtacttaac ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat
     7561 tctttgttgg atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc
     7621 atcatattct ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg
     7681 tctcaatgag cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat
     7741 tgctttgtct gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac
     7801 acgaccattc aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc
     7861 tcttaacaat agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc
     7921 tactacatct tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc
     7981 gacaagctga ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac
     8041 ttctgataat cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc
     8101 gccacttaaa cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat
     8161 cgtatcaaat tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca
     8221 aaaaaagtgc ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc
     8281 ttgttcttga ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt
     8341 aaaacaatta gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt
     8401 ggatgtctct ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt
     8461 atctttctta aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga
     8521 aaggagaaaa tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg
     8581 gatcaacgat gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat
     8641 cgtttttgtt gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga
     8701 ccttttctta acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc
     8761 agtaatcaac tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga
     8821 actttttgct tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca
     8881 gacaaaaaaa gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta
     8941 gtgactactt catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga
     9001 aaaactttgt caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg
     9061 ttctactact aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc
     9121 tttgcttaat gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac
     9181 aggctgccct gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc
     9241 ttggcgcttt tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa
     9301 acgatggatg aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc
     9361 aatcagtatg ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct
     9421 ttccattttg acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc
     9481 caattccgtt aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag
     9541 aatcgtttcg ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac
     9601 agtctgttct actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc
     9661 attctttcga cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac
     9721 ttggtctttg gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc
     9781 agcttcataa atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc
     9841 attgctttct taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct
     9901 tttcgaattt tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa
     9961 actacccagt ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca
    10021 ggcatcacga aattcatgat cttcattgat gcattcgatt caatttgact catgctagat
    10081 aaatacgtac ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc
    10141 gcgttcccta agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata
    10201 gattgccata acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat
    10261 ggattgacgc cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt
    10321 tccggatctc ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc
    10381 gttttacgca tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc
    10441 agtgtaaata aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca
    10501 gcaaagtaat aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca
    10561 gttccgcaac cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt
    10621 ttgtatttct tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt
    10681 ggactaaatt ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca
    10741 caataaaatc caactccgga tcaatttctt cttttataga ataaactgct gctcggattt
    10801 ttcgtttgac ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa
    10861 ctcgaaaatg aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa
    10921 aagaatctcc cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt
    10981 ttctcattag ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt
    11041 acgattgtcc tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag
    11101 tggcctaagc tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc
    11161 catttttagt actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg
    11221 gttggtatgt tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac
    11281 agacatactt tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc
    11341 gattgttaaa acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt
    11401 tatgcacagg gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt
    11461 aaaaaaagta atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat
    11521 atctgtattt tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac
    11581 ctgtgcatta gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt
    11641 tgtcgattgt gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact
    11701 attgaaaaaa atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta
    11761 tatggtatcc ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc
    11821 gcctgctagc tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca
    11881 gctcgttgtt gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac
    11941 aaaaatcgtt gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac
    12001 tggcgaagaa gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc
    12061 tagtccgcta aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg
    12121 taaaggaaac cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat
    12181 ttataacccg ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc
    12241 aattggtcat caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga
    12301 aacttttgct aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca
    12361 agaatatcga aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga
    12421 agctacgcaa gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat
    12481 cgtactaacc agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc
    12541 tcgctttgca tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc
    12601 gattttacgt aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta
    12661 catcgcagga caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca
    12721 agcttttgct gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa
    12781 aacgttaaaa tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt
    12841 ggcaaagtat tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat
    12901 tgttgttcct agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc
    12961 gaaaatcgga gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa
    13021 aatccagcag ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa
    13081 ctttttacta aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa
    13141 aaaacgttca aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt
    13201 tttttgagtt ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat
    13261 aataaataaa caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc
    13321 tttatgcagg aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt
    13381 ttgacaggtg taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct
    13441 gatatatcaa ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa
    13501 tctactggtt ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct
    13561 gaagaaacat ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa
    13621 gcgaatttta tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa
    13681 agccataacc agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt
    13741 tttgctgtct cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct
    13801 gataattctt tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca
    13861 gtagaacaag cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta
    13921 tctcgctcat taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg
    13981 ttgttcaaaa cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat
    14041 accaatcgtt tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt
    14101 ttagcggcga tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt
    14161 ttatctattc gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta
    14221 gaagaaagct tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg
    14281 gattatatga aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc
    14341 tctgctattc gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg
    14401 attactcctg tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa
    14461 agaaatagaa aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg
    14521 tttatttttg tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg
    14581 ataaatttgt tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat
    14641 gagggatggg cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa
    14701 atgttgaaag aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa
    14761 aacactgtgt tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc
    14821 atgatggttg aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat
    14881 aatgaggctg aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga
    14941 attttccaag aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt
    15001 ggaaagtatc tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt
    15061 gattggctgg actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac
    15121 tcttccatta gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga
    15181 acagaaaaag ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga
    15241 tctttcttta gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg
    15301 ccagatcaat ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag
    15361 aaaccagtac ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat
    15421 tttgtccgaa caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt
    15481 caagcgattg gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca
    15541 gctggaaatc gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat
    15601 ccaagaacaa tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac
    15661 aacgacagtt ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca
    15721 gacttatgga tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat
    15781 cgatttgatt aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag
    15841 cgagctggat gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac
    15901 gttcttaaca acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat
    15961 tttttatgtt caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt
    16021 ttagtagaac gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa
    16081 gccgttcgta aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac
    16141 cttgtttggg aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa
    16201 atccatgtga ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata
    16261 cctgtagata tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta
    16321 catgctggag gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta
    16381 ggatcttctg ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga
    16441 aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa
    16501 caaacggatc gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag
    16561 acaactgagt ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac
    16621 cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac
    16681 cattatgacg gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt
    16741 tttccagaac ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg
    16801 caatatacag acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat
    16861 gaaggtggaa cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat
    16921 gcccgaaaac aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga
    16981 gaaggattga cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca
    17041 aaaacaaaat tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat
    17101 ttcatgaaat tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta
    17161 ctagcttcca gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt
    17221 gctttagaga ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa
    17281 aaatgcgaat tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt
    17341 aatcgagagt ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca
    17401 agcatggaca agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca
    17461 gggtttggag aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact
    17521 gatgccgatg tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac
    17581 atgcgtccta ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta
    17641 aaacaaggga agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa
    17701 caagtcgttg cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt
    17761 ggagaaatgg atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg
    17821 gcccgcgtca gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg
    17881 gatcgagtag aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta
    17941 gatatttaat catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga
    18001 tgtcaatcta accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt
    18061 cgcacgtgca cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata
    18121 cggcatgaac gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt
    18181 cggagatgtc atgggtaaat accatcctca tggagaccgt gcgatctatg agtcaatggt
    18241 acggatggca cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg
    18301 ttctgttgac ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat
    18361 cgctacagaa atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga
    18421 tgatacagaa aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg
    18481 aacaacgggg atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt
    18541 agtagcggct atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga
    18601 agtattacct ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg
    18661 aagagcgtat gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga
    18721 gatgccaaat ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc
    18781 aaaattgatc gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga
    18841 tttgcgggac gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc
    18901 cagcgcatcc gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg
    18961 attcaatatg ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct
    19021 tgaaaactat atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga
    19081 aaaagcagaa gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga
    19141 tgaaatcatt gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat
    19201 cgaacggttc gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg
    19261 attgaccggt ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat
    19321 tgaagattta actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga
    19381 attagcagac ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga
    19441 ggtcttaagt ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac
    19501 aaacaatggt tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg
    19561 acgtggtgtc caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg
    19621 ttctacccat gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg
    19681 atacgaaatt ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg
    19741 tatcgattcg agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg
    19801 ccactatctc ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt
    19861 tgctaacatc cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt
    19921 caatgtgcta ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc
    19981 tgtcactttc aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg
    20041 gatacgcctg cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga
    20101 agtattagtg atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt
    20161 gaaaggccgc ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact
    20221 agctggattg accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt
    20281 cattatccgt ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg
    20341 tttgatgaag atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc
    20401 tgcagaacaa acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta
    20461 agctagcaag aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt
    20521 gaatttctct taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt
    20581 gtataattag agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac
    20641 ctaagagccg aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca
    20701 tgtacattat tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg
    20761 atgcaatctt gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc
    20821 gtttcgctta tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt
    20881 ctccatcatc agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata
    20941 tcattcgtca catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga
    21001 aaacgatgag aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg
    21061 aaagatccag atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct
    21121 gtgaatcgta attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg
    21181 atttggcgca aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt
    21241 gttactggac gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg
    21301 acagaagtag tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc
    21361 caacaaagcg gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat
    21421 aactctaatc aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct
    21481 gacccattcg gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg
    21541 aatttgatag gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa
    21601 agtagactac attgctgcta accatattga atatatcgat tataaagata tcgaattact
    21661 aaaaagattt atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc
    21721 taaaaaccaa cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc
    21781 tttcgttagc gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta
    21841 ttcaattcct ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa
    21901 gttatggtaa aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc
    21961 atttatcccc gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct
    22021 taattttacc atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg
    22081 ttcgctattg gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc
    22141 aggcttcgac tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag
    22201 tgattgctta tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa
    22261 tcaaagatca aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag
    22321 aaaaaaagaa aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag
    22381 taaagggtgt tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga
    22441 cgaacagtca gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata
    22501 aattagatga caaagagatt tcttatttga actctttcgt cactacacta gtttctgact
    22561 ggatgaatga ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag
    22621 cacagatgat cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc
    22681 gagcagaagc tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct
    22741 caagaaccct cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc
    22801 gtggaggcga ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg
    22861 gggggaaatc tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg
    22921 ctttgcgtcg gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg
    22981 atatggatgc tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa
    23041 aagcttacat cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag
    23101 aaatccataa gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc
    23161 tgaaaaaaga agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct
    23221 ctcagggcct atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg
    23281 aagagtttcc aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag
    23341 agctagtttc tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg
    23401 aggccacatt attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa
    23461 ctgcacgaac ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag
    23521 tacaatattt attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg
    23581 gaagcgacta tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata
    23641 atagtgttat agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag
    23701 ctttcgtcat tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca
    23761 cgatcaacgt ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag
    23821 ctactcaact ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca
    23881 aagaaaatat gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca
    23941 agatgtaaaa ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca
    24001 aaattatcta atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt
    24061 agcaggacaa aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa
    24121 gttaaaagaa ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga
    24181 agacagccgt ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca
    24241 atacaaaatc aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg
    24301 ctacacaaac gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt
    24361 cgtacaagaa taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa
    24421 aaagacaaac cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa
    24481 tcgatttcag attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg
    24541 agtataataa aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc
    24601 tcattttctt gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg
    24661 gcaggatcga atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt
    24721 tcttgacgca gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg
    24781 tcgtggccat caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat
    24841 tgacgtggtt actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg
    24901 tttaagttat ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta
    24961 tgcgaaaatc gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat
    25021 cgttacgaaa ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg
    25081 cagtatcttg gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt
    25141 actgaactcc acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg
    25201 tctaccaact ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact
    25261 aatcattctt gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca
    25321 aaatgtagga acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga
    25381 atctcttgta aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac
    25441 aggtcagctt tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg
    25501 agcaaatatt tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg
    25561 tcggaaacta gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt
    25621 gattgaaggt aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact
    25681 gaaaaaactg gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg
    25741 agtagaacag cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat
    25801 tgaacaagat gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg
    25861 cggagatgac gatgatggaa acgagccaaa aaatgacaac atcatcgaag tcatcatcga
    25921 gaagaaccga agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa
    25981 gttttcgtcg ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat
    26041 cccgagagct ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat
    26101 ttaaacagat ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata
    26161 aacggtgttc aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac
    26221 tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc
    26281 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag
    26341 aagcagtttt ttgaaattgc tccttgtgag tcagagttaa acggcaggct cacaacctct
    26401 ttcttaaacg gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata
    26461 tgagaagcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
    26521 ctctgattca cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa
    26581 atttgagaag caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac
    26641 aacctctttt ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa
    26701 aataaaaata aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt
    26761 tcaggattaa ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg
    26821 caatggggcg atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg
    26881 attgcacggt accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact
    26941 tataaactgc atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga
    27001 aacggcgttg tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa
    27061 ggtgtagaaa caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat
    27121 atcaaattgg accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt
    27181 aaaggaatcg gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac
    27241 ttattagata aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt
    27301 caatttgtaa aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat
    27361 tatgaatacg gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat
    27421 gctttggatg caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc
    27481 gaccagggaa cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc
    27541 ggaagcggtg tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg
    27601 tcacgtgtcg gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc
    27661 cgcgaagtag gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt
    27721 gattctgtag ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac
    27781 tcgatcgatg tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat
    27841 ggcgaactaa tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc
    27901 tatgaagaat tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg
    27961 ccagaaaacg cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca
    28021 acattttctg tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa
    28081 atctaaggat aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt
    28141 tatctatacg ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg
    28201 ataggctgtc tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    28261 cagtcccttt ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat
    28321 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    28381 gctttttcac aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca
    28441 caatattcaa caaacatgag aagctgtttt ttga
//
LOCUS       DACEKG010000035        26352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEKG010000035 DACEKG010000000
VERSION     DACEKG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.035"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1228)
                     /locus_tag="IW664_001427"
     CDS             complement(98..1228)
                     /locus_tag="IW664_001427"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0848796.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IW664_001428"
     CDS             complement(1877..3079)
                     /locus_tag="IW664_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0848797.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IW664_001429"
     CDS             complement(3139..3342)
                     /locus_tag="IW664_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0848798.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IW664_001430"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IW664_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IW664_001431"
     CDS             4879..5691
                     /locus_tag="IW664_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848799.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6912)
                     /locus_tag="IW664_001432"
     CDS             complement(6715..6912)
                     /locus_tag="IW664_001432"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848800.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IW664_001433"
     CDS             complement(7058..7936)
                     /locus_tag="IW664_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0848801.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IW664_001434"
     CDS             complement(7975..9093)
                     /locus_tag="IW664_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0848802.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IW664_001435"
     CDS             complement(9090..11189)
                     /locus_tag="IW664_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0848803.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IW664_001436"
     CDS             complement(11240..12061)
                     /locus_tag="IW664_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0848804.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IW664_001437"
     CDS             complement(12058..12867)
                     /locus_tag="IW664_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0848805.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IW664_001438"
     CDS             complement(12888..13382)
                     /locus_tag="IW664_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0848806.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IW664_001439"
     CDS             complement(13394..13882)
                     /locus_tag="IW664_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0848807.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="IW664_001440"
     CDS             complement(13959..16655)
                     /locus_tag="IW664_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0848808.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="IW664_001441"
     CDS             complement(17083..18156)
                     /locus_tag="IW664_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848809.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="IW664_001442"
     CDS             complement(18153..18980)
                     /locus_tag="IW664_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848810.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="IW664_001443"
     CDS             complement(18981..19787)
                     /locus_tag="IW664_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848811.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="IW664_001444"
     CDS             complement(19799..20884)
                     /locus_tag="IW664_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848812.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="IW664_001445"
     CDS             complement(20901..21443)
                     /locus_tag="IW664_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0848813.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="IW664_001446"
     CDS             21701..22690
                     /locus_tag="IW664_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0848814.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="IW664_001447"
     CDS             22687..23424
                     /locus_tag="IW664_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0848815.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="IW664_001448"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="IW664_001448"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0848816.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="IW664_001449"
     CDS             23988..24620
                     /locus_tag="IW664_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0848817.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="IW664_001450"
     CDS             24705..25352
                     /locus_tag="IW664_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0848818.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="IW664_001451"
     CDS             25357..26340
                     /locus_tag="IW664_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0848819.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8059 a         5571 c         4466 g         8256 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatc
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacggtc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg at
//
LOCUS       DACEKG010000036        26293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEKG010000036 DACEKG010000000
VERSION     DACEKG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.036"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(97..1464)
                     /gene="nhaC"
                     /locus_tag="IW664_001452"
     CDS             complement(97..1464)
                     /gene="nhaC"
                     /locus_tag="IW664_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0848820.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1573..2994)
                     /locus_tag="IW664_001453"
     CDS             complement(1573..2994)
                     /locus_tag="IW664_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0848821.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3237..5114)
                     /gene="tdc"
                     /locus_tag="IW664_001454"
     CDS             complement(3237..5114)
                     /gene="tdc"
                     /locus_tag="IW664_001454"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0848822.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5405..6661)
                     /locus_tag="IW664_001455"
     CDS             complement(5405..6661)
                     /locus_tag="IW664_001455"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0848823.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7241..8542
                     /locus_tag="IW664_001456"
     CDS             7241..8542
                     /locus_tag="IW664_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0848824.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8635..10023)
                     /gene="ltrA"
                     /locus_tag="IW664_001457"
     CDS             complement(8635..10023)
                     /gene="ltrA"
                     /locus_tag="IW664_001457"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0848825.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(10510..11109)
                     /locus_tag="IW664_001458"
     CDS             complement(10510..11109)
                     /locus_tag="IW664_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0848826.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            11363..12244
                     /locus_tag="IW664_001459"
     CDS             11363..12244
                     /locus_tag="IW664_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848827.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            12257..13867
                     /locus_tag="IW664_001460"
                     /pseudo
     CDS             12257..13867
                     /locus_tag="IW664_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(13944..14603)
                     /locus_tag="IW664_001461"
     CDS             complement(13944..14603)
                     /locus_tag="IW664_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0848828.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(14886..15551)
                     /locus_tag="IW664_001462"
     CDS             complement(14886..15551)
                     /locus_tag="IW664_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848829.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(15698..16303)
                     /gene="dhaL"
                     /locus_tag="IW664_001463"
     CDS             complement(15698..16303)
                     /gene="dhaL"
                     /locus_tag="IW664_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0848830.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(16331..17314)
                     /gene="dhaK"
                     /locus_tag="IW664_001464"
     CDS             complement(16331..17314)
                     /gene="dhaK"
                     /locus_tag="IW664_001464"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0848831.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(17316..17711)
                     /gene="dhaM"
                     /locus_tag="IW664_001465"
     CDS             complement(17316..17711)
                     /gene="dhaM"
                     /locus_tag="IW664_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0848832.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            17865..18185
                     /locus_tag="IW664_001466"
     CDS             17865..18185
                     /locus_tag="IW664_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0848833.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(18297..19166)
                     /locus_tag="IW664_001467"
     CDS             complement(18297..19166)
                     /locus_tag="IW664_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0848834.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(19262..20566)
                     /locus_tag="IW664_001468"
     CDS             complement(19262..20566)
                     /locus_tag="IW664_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0848835.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(20563..21072)
                     /locus_tag="IW664_001469"
     CDS             complement(20563..21072)
                     /locus_tag="IW664_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848836.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(21247..23382)
                     /locus_tag="IW664_001470"
     CDS             complement(21247..23382)
                     /locus_tag="IW664_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0848837.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(23465..25120)
                     /locus_tag="IW664_001471"
     CDS             complement(23465..25120)
                     /locus_tag="IW664_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0848838.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(25122..25436)
                     /locus_tag="IW664_001472"
     CDS             complement(25122..25436)
                     /locus_tag="IW664_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0848839.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            25606..25866
                     /locus_tag="IW664_001473"
     CDS             25606..25866
                     /locus_tag="IW664_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0848840.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            25880..26194
                     /locus_tag="IW664_001474"
     CDS             25880..26194
                     /locus_tag="IW664_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0848841.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         7385 a         5494 c         4754 g         8660 t
ORIGIN      
        1 agctgaaatt cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag
       61 ttaaacgctt ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt
      121 caataaaaaa ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg
      181 caagtaatgc aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca
      241 cggtacgaga gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt
      301 tgataagcca acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag
      361 atactgttct ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa
      421 acgagaaggt cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata
      481 tttgatcagt aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat
      541 actcatgatc ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac
      601 ataaccattc atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa
      661 agaaagaacg agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt
      721 gattgccccc ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct
      781 cgctttcatg ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg
      841 tcctaatatc gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat
      901 accagcgata tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc
      961 tgataaaggc gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat
     1021 tgcatcattg aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat
     1081 cgttgtaaat gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg
     1141 taaaaagaaa cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc
     1201 agataaaatc catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat
     1261 ccctggagtg attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa
     1321 tctgccataa aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg
     1381 gaatccgatg atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc
     1441 ttttaaagaa atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca
     1501 tgaaaaaact attcacttgg caggacctcc tactaaatca gcgacaaaac gtcggctgaa
     1561 agacttattt ttttattttt cttctaaact tttttctacc tcaatccccc aagaatcttt
     1621 cttgaagtga ttgatgatca atggaataac gaacatgacc actgtgatag ctgctactaa
     1681 taatgcatca ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga tgatagagaa
     1741 gatcagcatg cagctgacta cccaagctaa tccgtttccg tttttaccaa ttcggtaagg
     1801 acgttcgaat tctggatgtt tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta
     1861 cacgatacaa taaataacag ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc
     1921 atccattaca ccataaagca aagcaaataa tgaaataacg attgattgtg ttagaacgac
     1981 atttctagat acgccgtatt tgttttcttt atggaatccg aattttggtg gaaggaatcc
     2041 ttcacgagct acttggatga ttgtttttga tggtccagta acccaagctg ataattgtaa
     2101 aagcacacca ataaatacca tgaagctgaa gatatttccg atcacttctg gtaagcctag
     2161 gatttgacag tcaatcaaga taggctgcgt gatatttgct aattccattt ttccatctgg
     2221 tacgacattt gctacaagca tcgcattgat gacattcaat aaaactaatc cgatcaaagc
     2281 gataaatacg ccttttgtat aattttttgt tgcgtctttc aaacgaggca tgtaaacaga
     2341 actcatttcg atcccaacaa aaataaatgt gataccagct aagtatttca atgaatcaat
     2401 atgttccaag tcagggaaag cttttgacca tgaaaagtca cctagatagc cgtttgagac
     2461 tagtccgact ttgaaggctg ccaaaacacc taaaacgaac atgatgacta ctgggatata
     2521 tacccctagc catacaccga tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa
     2581 gatcgtgatg atccagtaga taactaaaat acaacctaaa acgaaccagt gattattgcc
     2641 aagttctaca ttaccaaatg tgtttcctaa caatggtcct aaagtagaag cgaccattac
     2701 catacctggg aacatttgca cccataatag ccatgcaacg acaaatcccc atttgctgcc
     2761 cagtgctgtt tttacccata actgaggacc gccttcttga ggaagcatcg tggaaagctc
     2821 accggaaacc attgatatcg gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat
     2881 caatgtccaa ccgactgcgg ctaacgtagg aatactccga accgttgcac acagtgccat
     2941 tgtcagaccg ataaaggtcc caagacccat tttacttact tcagatttac tcaccgttga
     3001 cactctcctt gattttttgg ataaaataac ggtgtctttg aagtagtttc agcaataaac
     3061 gaaacaattc agcgaatatt tgaggggatt ctttgagttt attgcttgaa gggtttcttc
     3121 aaagacaacc tcaaaacaaa gggatcgaag agcttaggtc tccccttgct cttcagatcc
     3181 aaacatgcgt cagaaacagt tagtattcaa gctgttttct aacatgatta ccttgcttat
     3241 tttgcttcgc ttgccaataa ttggtctgcg tagatttttt ccaatttttc ttgaagagct
     3301 gctttgattt tttcagcata ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt
     3361 acgctggcac gcaatacagt tacttttccg gcacggttcc attcttcatc tgagaatcct
     3421 agttggttca caaattgtaa tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt
     3481 gaagttaaga actcattacc gtaaatgctt cctttcacgt atgaagaata atcatatacg
     3541 tcatggttca atttattcat tgcaactaag tcatcgttgc ctttttcttt gaatacatag
     3601 tctaccatat tgaaatctgg ataagttaat ggatgaactt cgatttcttt gtctcctact
     3661 ttaaatgaca agtcattcaa gaagttgtag aaacgatgtg caccttcgat tgatgcaccc
     3721 attaatttac catatcctgt gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg
     3781 gctgctgttg cccctgcttt tgaaccttcc aagatataag ccccaagtaa tgctgggata
     3841 tctgcgcctt tttcaaatac atatgtcgca aagtaagaga taacgtctct catgcggata
     3901 tctttgatga cgataccacc ggcagaatat ggaacatagc ccattttatg tgggtcaatc
     3961 gttacagatt ctgcttcttc gattgcttta tatgcactgt gtacttcttc taagatataa
     4021 tctttgtttt ctgtaaagac attgtatttg taatgaacat cttttaaatc ttcaaatgga
     4081 atgaagttgt tgtcttcgtc taagaaaatc gcacggccgt atccgccata agcagcatct
     4141 acgtgtaagt agaagtaaat accgtctttt tctaatacgc gacgcaatgc aacgatttta
     4201 tcgataccgt cgattgctcc ttcttctgtt gatcctacaa caccgactac acctaggatt
     4261 ggtgtttttt cagctgctag accacgaacg attttttcaa gttcgttgat gtccatgcga
     4321 tagttgtggt caactggcac tggaatcact tggtctaaac cgataccgat gatgtcggca
     4381 gcttttagcc atgaatagtg tttagtttgt ggaaccaacc attttcctaa tttttctaaa
     4441 tgttttccgc tacgtgctga gtgagctttg atttcgtcga ttttttctgg gacgctgtct
     4501 aacagattca tgatttcttc tgttgacaag ttcattaatt cccaatcgct ttttccagca
     4561 actagttcag gtgtcacttc tttcattgca agcggcaatg atttgatgtt gcgggcatac
     4621 caaagtcctt ctaagtttgc taaagaacca tcagcaacga tgtggcccca gccatcttta
     4681 tagctcatta attttgcaaa ttccattcct acttcttctt ccatttggct tgttgcagga
     4741 gaagattcat aggcaacgtt attaccattc catagcatag caaaattgta cgctagtaaa
     4801 gatggcatca atgtttcaga gttcatatga ccccagtaac gtccggcatt atgccaagga
     4861 acggaatggg tacgcatccg cgctgaaatt tctgatagaa catcctttgt tttattcaca
     4921 gtgtgttcaa aactagcact gcttttttct tctggcgtaa taataggcat gtcttgaggc
     4981 atgtagtttt gacgccagcc taaatgttca tccaccaatt cattcaacaa agctttatag
     5041 atttggccgt tctcagcttt gtccccaatg aatagggcat ttaggtttag atctttcgac
     5101 aatgattcac tcatagtgaa accctcctaa aaaaatattt aaattttgtc gagccctaca
     5161 ttacacctgt tcttctattc tgtaaactaa gttttggcct gatttttaat catattttaa
     5221 tatgtatcaa ttatccgaat tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt
     5281 tacaaatgta aactccctcg caaagattgt attaacgaca accagcgttc tattttagga
     5341 tgggtttcaa aattggaatg ttgtaatagt atttcccttt ttcaaggtaa aaacttacta
     5401 aattctaatc aattactttg gccaaaaagt aatttttctt accttttcga ataaccacaa
     5461 acttaccatc gaattcatcg gatggattta tgacaaaatt taaatcggta acgcggtcac
     5521 cgttaatact tatagctccg tttgaaacat cttcacgtgc ttgacgttta gatggttcaa
     5581 tttttgtgga aacaagcagt tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg
     5641 gcattttacc gaatccttgc gcgatttctt ccgcatttag ttctttaata tttccagaga
     5701 acaatgcttg cgttattttt tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg
     5761 taacttcttc tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc tctacttttt
     5821 tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa gaatttcaag tatttcacta
     5881 catcgcgatc atcttgattc aaccagaatt ggtagaactc aaatggtgaa gttttcttcg
     5941 gatcaagcca aacggcaccg cctgcagttt ttccgaattt cgttccgtca gccttcagca
     6001 tcaatgggat cgttaaaccg aacgcttctg cttctggtcc ttctaatttt cggattaggt
     6061 ctaatccagc tgtgatattt ccccattggt ctgctccacc gatttgcaat tgcacatcgt
     6121 atgttttatg cagatgcaaa aagtcgatag attgcaggat ttggtaagta aactctgtaa
     6181 atgagattcc tacttccaaa cggctggcaa cgatatcttt tgctaacatc gtgttgatgt
     6241 tgaagttttt accgtagtct ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt
     6301 agttgttcac aaatgtgata tttgcatctt taccaaacaa ttttctcatt tgattggaaa
     6361 gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa cacacgttct gttttgcggc
     6421 cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag aatatatgga tgatggcctg
     6481 ccaattggaa tcttttcatc atcataaatg gaatcaaatg tccgatatgc atcgaatcac
     6541 cagttggatc aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc
     6601 tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt taattcatca ataatattca
     6661 ttttactgac ctccgtgtag tttttttatg aaagcaggaa cctgctagca tgtgtaatta
     6721 attatttctt caggatagga taggaaataa aaaaagtccc ttggcatata agccaaggga
     6781 cgaaatctcg cggtaccacc caatttgtat gtctagatat ccaacatacc tctcgaacat
     6841 ttaacgctgt cgcgtggcaa tcactgccaa agactcctga aatgtaattc gctttaaagc
     6901 atgtactggt ttgcagcgac caccagcttt ctgaaacatt ggctttaaca actacttcgt
     6961 ttcgatcttc gtcttgttat tattaatatg tgtctatcat agagcgcaac tttttctttg
     7021 tcaacagaaa aaggatattt tttttaaatt cctcattttt tcttttttga atccgctcta
     7081 attccgaaca ttttattttc aattattgtt aaaagaaata actttttctg tattttttct
     7141 gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta caataaggaa
     7201 tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat
     7261 tattttagga gccacttctg ctttaactat cgttatttta attctaagtt tgttttttgt
     7321 catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc
     7381 aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc tggctcaaag
     7441 ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg gatctactga
     7501 aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg actggtctga
     7561 tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc aaaaagcctt
     7621 ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag aattcaaccg
     7681 ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg atttgatcca
     7741 aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc
     7801 attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt ccggttttcc
     7861 tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag agaaagataa
     7921 agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca agaaacatcc
     7981 tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca acggagtttt
     8041 aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa
     8101 agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg cttcccatac
     8161 atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag acaatgaatt
     8221 gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt atccatttgg
     8281 tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg cttacttgaa
     8341 gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt ggggacaatt
     8401 aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt ttcaatctga
     8461 tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg tctatgacaa
     8521 agaagcacgc gaatctgctt gattttacgt catttatgaa tcgagtagag gggaaagcca
     8581 actgaccttc cgcccctctc acaccaccgt acgtacggac ccgtatacgg cggttcaata
     8641 agttgagtgt acgaactggt acttcaagga gatgtcctca tatccttggc gggccagttc
     8701 tttgtctgtt aatgatcgat gaagaatatg acttttggag actgaccaaa taccttttct
     8761 tgtatttgac cattcatacg ctttcgcttt atcgatccct aaacgaatca gattttttct
     8821 tttggttctt ggattcttcc attgtttcca aatatattgt ctgattcttc gcttcaacca
     8881 accgtttaac tccctcatga attctttcat ctcaccaatt ccatagtagt tgatccaacc
     8941 agtcattagt tgcttgattt cttttaaaat aagttggata cttcttccac ggtttctttt
     9001 agtgagttgt ttgagcttgc ttttcacacg ctgttttgct tttgcgtgag ggcgaatttt
     9061 aatacctttc tttgttggca gaatgcagaa tccaagaaac tttcgtttca atggacttcc
     9121 taccgcactc ttttctcgat ttaccgtcaa ctttagttgt tcttcgagaa attgaatgga
     9181 tttcttcatc acacgctgtc ctgctcgttt gcttctaaca tagatattgc agtcatcggc
     9241 gtatcgaaca aatttatgtc ctagattttc tagcacttga tcaaattcgt tcaggtagat
     9301 gttacttaac aggggagaga gattgcctcc ttggggcgtt ccttcttcag ttgttgtgat
     9361 gagtccattt tccattacac cactcattaa gtattgacgg atcagtttta ggacttgttt
     9421 gtcctttact ttccgctcaa tgaaatacat cagcttatcg tgatttacgg tgtcaaagta
     9481 ggctttcata tcaatgtcca ccacatatcg gtaaccttgt tcataataag attttgcttt
     9541 cttcactgcg tcatgtgcgc tgcgatttgg gcgaaaccca taactattat ctgagaattc
     9601 tggttcaaat agcggttgta acacttgatt gattgcctgt tgaagcatac gatcaataac
     9661 cgtgggaatt cctagttttc ttacaccgcc gtttggtttc ggtatttcta ctcgcaaaac
     9721 aggctgaggt ttatagttcc cttggcgtaa attggatagt aattcctcgc ggtgctcttt
     9781 caagtaagga agcagttgat cgacggtcat cccatctgca ccagccgccc ctttatttct
     9841 gactacttga agatacgctt gattgagatt attccgatct agaattctcc caaacagatt
     9901 ttctacacca tcttgtttcc tctgttcacc gcaagaagtg ctgcgcactt catcataccc
     9961 ttcgtgttcc acactagctc tttgatgata gccaagtttt cttgttttct gcggtcgtcg
    10021 catcttctca cacctcctaa attattcaag attcttattg ttcagtcctt cgtaacggtc
    10081 agtgtcgtta ctactatgac ctcggctgac ttctcatgat tcaccatcat gtcgccatga
    10141 tcgttgtgct ggtttgtttt cattcaacgc ccacaacacc ctcatgagat ctcccccggt
    10201 aagagtgaaa actttcctcc catgtaactg ctagatttac tgtataggtt tcgggcagta
    10261 ttggactttg tcttgtgatg aagacttatc cgacctaatt cagccttgta tctagtttct
    10321 gttcgtcagt tcaggagttt gcgtccgact tccttcagcc acaacctcac gattgccgcc
    10381 ttgtctttcg ctaacagttc ctactgccaa gcctgtagtg gactttcacc accaagtttt
    10441 cacccatgca gggcgcacac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
    10501 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
    10561 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
    10621 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
    10681 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
    10741 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
    10801 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
    10861 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
    10921 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
    10981 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
    11041 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
    11101 accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt
    11161 tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt
    11221 atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaagt
    11281 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
    11341 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
    11401 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
    11461 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
    11521 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
    11581 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
    11641 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
    11701 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
    11761 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
    11821 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
    11881 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
    11941 cgagcgacta gcggataaag tcgtgatcat acgcgaagga acactcgatg aagaaggaac
    12001 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
    12061 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
    12121 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
    12181 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
    12241 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
    12301 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
    12361 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
    12421 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
    12481 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
    12541 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
    12601 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
    12661 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agttgtacga tgattttttt
    12721 ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
    12781 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
    12841 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
    12901 tgtagctgat tcctcccttt cgatgtggaa acctttaggc tggtcttatt tgacctatcc
    12961 atttacaact aacaactgaa ttcctttgct ttatttgttt ttgttcagtc taatcttgat
    13021 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
    13081 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
    13141 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
    13201 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
    13261 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat
    13321 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
    13381 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
    13441 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
    13501 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
    13561 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
    13621 cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact
    13681 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
    13741 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
    13801 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
    13861 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
    13921 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
    13981 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
    14041 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
    14101 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
    14161 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
    14221 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
    14281 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
    14341 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
    14401 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
    14461 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
    14521 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
    14581 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
    14641 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
    14701 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
    14761 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
    14821 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
    14881 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
    14941 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
    15001 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
    15061 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
    15121 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
    15181 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
    15241 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
    15301 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
    15361 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
    15421 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
    15481 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
    15541 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
    15601 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
    15661 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
    15721 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
    15781 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
    15841 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
    15901 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
    15961 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
    16021 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
    16081 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
    16141 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
    16201 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
    16261 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
    16321 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
    16381 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
    16441 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
    16501 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
    16561 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
    16621 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
    16681 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
    16741 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
    16801 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
    16861 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
    16921 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
    16981 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatcgg
    17041 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
    17101 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
    17161 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
    17221 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
    17281 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
    17341 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
    17401 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
    17461 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
    17521 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
    17581 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
    17641 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
    17701 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
    17761 attaatgaac aattcaagta tataataaat ttctaatttt gggaaaatat gtgtagtgct
    17821 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
    17881 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
    17941 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
    18001 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
    18061 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
    18121 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
    18181 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
    18241 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
    18301 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
    18361 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
    18421 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
    18481 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
    18541 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
    18601 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
    18661 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
    18721 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
    18781 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
    18841 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
    18901 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
    18961 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
    19021 gcaccagaag agcttatggt tgttgaaggt tctgtttgag caggttcaga agtttccgca
    19081 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
    19141 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
    19201 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
    19261 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
    19321 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
    19381 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
    19441 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
    19501 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
    19561 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
    19621 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
    19681 tcagccagac ttctgttcca ccttttgccc aacgttttct gtgtcgatat agcatcttca
    19741 acgtcacagg aacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
    19801 ttaactgatg atcccaagca atactgattt cttctgtcgc tcgatcttgt ctgaatccgc
    19861 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
    19921 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
    19981 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
    20041 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
    20101 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
    20161 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
    20221 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
    20281 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatattcga
    20341 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
    20401 gaacatctac ccattctttt tgcttatgtt tgtatataaa cacataacac cagacaccaa
    20461 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaaatac ataatagaca
    20521 aacaagtatc taagaataaa ttacctgtat gggtgattga gatcatttca gttccacccc
    20581 attttcttta tacaaatctg caatgaatgt cgtatctaga tttttctctt cggagacaga
    20641 ataaaaacgg accgtttctc tctcttcttt tgttccaaaa cgttccgtat acacttcttc
    20701 aagtaattct tgtctttttt tcaatttttc atcttcatat tcaaaagatt tatttacttt
    20761 tttgtataga tttcgattgt tccacctagt caatacaaat gaggtaatca ggaaactgaa
    20821 aaaaatcaat acaaaaaagc cgattaagaa atacagcatt ttcttttctg ataaaaaagc
    20881 gtacaaggaa aaatccttgg ctctttctgg tctcgttaat gggaaaaaga cccatataaa
    20941 aggaagaaca acgatcatcc atcctaaaag agccactaac gtttgccaaa tctttaacca
    21001 gtaattacct tttgaaaaat acttatcttt caccaaaatt gatgcaactt cttctcttgt
    21061 tacttttttc atatcctcac ctcacgtttt tttgttacga cgtaggagtt taccatacat
    21121 ttcatttctt ttcacataaa gtgttcctaa atttttccta agaaaaagaa caaaacattt
    21181 ctcatcagca aatgaaaaat attttgttct ttttatcagt tgttcttttt attttttatc
    21241 tcttaattat gttgctcttt ccaatacgac aatgcttttc attcctctag atatcgccgt
    21301 gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt tcaaatggat ataagattac
    21361 atgatcaaat tccagtcctt ttgctagact caaaggaatg atttggcagt tttctaatcc
    21421 atatgaattt tttttaatcg attcttgtag cgtttgggtt tcctcttctg tttgggtgat
    21481 aactgccgct ttttctcctt ttagagaaac caaaatctca gctagtattt tcaacaattc
    21541 ttcagaggat cgaaaagagc ggtattcggg ttcttttcct tgcggtcgga tcgatacgat
    21601 gtcgatcttc tcttgatcca cagcataggt tttgaaaagc tctgtgattg ctccgctaga
    21661 acggtaacta tttctaagat cataccgatg gatcggcaga tggtagtcgt cgaaacaacg
    21721 cataatatca gcaaaagtag tggaagaatt aaagattgcc tgattctcgt cacctactaa
    21781 agtaaagtga gtttttggaa ataattcgat caatagacct aattgtgcca gggtataatc
    21841 ttgtacttca tcaatcaata gatagcgaaa tgctggtaca tttacctttt caacaagtaa
    21901 atgacgaata gctaatataa tgactgcttc atcaacagta cgtgtgggtt gccaagcata
    21961 agctgagcca tatcgttttt cataaattct ttcaaacaac tgatgctcat ccacccatgc
    22021 catttcttcg atttgtctgg ttatttttcg atattttttt tgtaaaagtt tccttgcata
    22081 agctacaata ctttgttccg aatcatcaga aatcaattta ccgaataatt ccaactgcct
    22141 atcttctgtc aacgagagta cctgattctg cacagcagga ctttttgctt gtttcaaaag
    22201 atgattcttc cattcgctca tcagtgcctt tttcgttgct tgaagcctat cgatcaattt
    22261 aggatgagca ggaactttct catatatttt ttcgatcgtt tttttagaaa ggactgtttt
    22321 tccattttta gtaagtccac ggataaaatt cggatgatct aaaaacaaag gatctgattg
    22381 ttttagatta tcgataaact ttttgctacg caaacgttct gtctgctccg atacattttc
    22441 tcccgtgata cgtttgaaat aagcctcttc accttctatt tcctctgctg aaagctgtga
    22501 aaccagctgt atgatggtta gattcagtgg atttctttca cctaatgatg gtaggacatt
    22561 cgaaatatag tcgataaacc gatgattagg tgatagaatc agcaagtcat cggatgtcat
    22621 cgtatctcga tataaataga gcagatacgc aatacgctgc atgatagcag aggttttacc
    22681 acttcctgct acgccattta ccaagatatt ttgacttttc acatcccgaa tgattgtgtt
    22741 ttgttccgat tggatcgtag cagtgatagc cttcatttca ttggtctcat cttggcctaa
    22801 gacttctaat aatacgtcat cttgaatagc tacagacgta tcaaaaaact tgaccaaacg
    22861 atctctttcc aaaagaaatt gtcgtctttc tttaataacc gtatcgattt tattttgacg
    22921 aaccgtataa gaagatggac caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg
    22981 ccaatcataa acaagatttt catctgcatc atctgtgaaa tttgctgtac cgatataaaa
    23041 ggattctttt ggctcattat ccagaaagtc tacaacgact tttccaaagt agggactttt
    23101 caataaacgt tcgatctttg ctagctgatt ttctgcgttt ttctgtttga tattcatctg
    23161 atcgatttct ctatttttca tttcaatcat cgaaaaagta tccaggttat ctaagtaact
    23221 gtcaaaattc aatcgaagct cgccagccat ttctttcaac gactggtttc cttctatttt
    23281 tgccgtatcc atcgattccc gcagttcgtt ttttttatca attagctttt gatacacgtt
    23341 cttcagatat gcttcttcta actgtttttg tgtgttgacc atacacattc tcctcttttt
    23401 ttaaagtggt ggaaccgatt agtttacctc ttttaagagt cgaatgcaag ctttttaacg
    23461 agtatcattt gttaggatat atcagttctt caggtcggac agtttgatat aaagaaaatg
    23521 caccatcaag gttttttacg tggtagccag actgcttcaa gattctttcc gctagatagc
    23581 tacgtaatcc gctatgacag ctgacgatat attcttgttg gctgtctaat tcatttaatc
    23641 ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt gaatttgccc ttttccaatt
    23701 cttctgctgt tcgcacatct aataaaattt ttccttttgc tagttctgtc ggcaattcat
    23761 gccattgtac ggtttcacta agcccttctg caatgttcat tgctgcatat cctagcatgt
    23821 ttactggatc tttggctgag ccaaatggcg gtgcatacgt caattctaat tccggtaaat
    23881 cgaagatcgt caaatgccct ttgattgctg ttgctaaaat atcgatccgt ttatccacac
    23941 cttttgcccc aacaccttgt gcaccataaa tctcgccagt ctctggatgg aagatcaact
    24001 tcagtaaaat atctgttgct tctggataat agcttgcatg atcttttcct gatacatgaa
    24061 tgacttcaaa agacagttcg ttttctttgg ctgttctttc acttaaaccg gtggatgcgg
    24121 cagtcattcc aaacgttcgt ataattgctg ttccaatacc gccttgattt cttcgagaaa
    24181 ttccggaaat cgtgtccgca acttgtctac cttgacgatt ggctggtgaa gcaagagaaa
    24241 taagtgcatc ttttccagtg atttgctgtt ttacaacgat agcatccccc accgcataaa
    24301 tatcaggaat attcgtttga taacgttcgt ctactaggat acccccgcgt atgcctaatt
    24361 cgattccagc ggtcactgct agctggttat caggacgaac accaatggat aaaataacta
    24421 aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc
    24481 cagcgacagc atgagaagta atcacttcta ctccttgatg agacaattct ttttctataa
    24541 aagctgccat ttcttcatcc agtgttggaa gaatatgtgg tgcttgttcg atcaccatga
    24601 cttctaaacc tcttcttttt aggttttctg ccatttccaa tccgatgaat cctgcaccaa
    24661 cgatcacggc acgctttggc tgtttttcta atgcatgcat cactttatcg atatctggta
    24721 catttctaat agaaaaaaca ttgtcggctt ctgttatccc tggaatcgat ggaacaactg
    24781 gttttgcacc aggagataaa accaatgcat cgtactgttc aatgtgtttt tgaccctcat
    24841 gtacgacttc taccgttttg ttttcgggaa agatggctgt cacttcatga tttggacgca
    24901 catctaaaca aaaacgagcg gataatgatt ctggcgtttg gacaagaaga ttttcccgtt
    24961 cactgatttc tcccgaaaga taataaggca agccgcaatt tgcaaaagaa acatacggtc
    25021 ctttttcaaa aacaacgatt tctgcatctt ccattaatcg gcgaagccgt gtagcagcag
    25081 acattccacc tgctacacct ccgacaataa cgattttcat cttatttccc tcctactgtt
    25141 cgatcaaacc attgattcat gccaccacgt acattcacta catcataacc agatttcttt
    25201 aattctttag ctgcttgttt actcctcatc cctgattgac aaatcacata gacggtcttt
    25261 tctttgtctc catcatatct gtttatactt tgtaatggaa catttttagc tccttttata
    25321 tgcccgcccc ggtattctgc aggtgttcta acatcaagta acgctggatt ttccaaaagc
    25381 ttttccttta attctgggac tgttatcgat tttattgatt gaaatagcaa attcattttc
    25441 tgcacctcat ttacattttt ataaatacct catggggtat attaaacagg agctttatta
    25501 atatgtcaac cagaatgact cttcattttt ttcactactt tttatatttg cataaaatac
    25561 cgataagggg tattatctaa ttataaaaat agaggagaat gtgttatgga gattgataat
    25621 aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg
    25681 atcgaagacg aaaaagaatg tatggatgtc atcactcaat taagcgctgt tcgttctagt
    25741 attgatcgcg taatgggcat gatcgttgca gataatctga aaaattgctt tgagaaacct
    25801 gatagtaatc cagaagaaca agcggctaaa ctagaacaag cgatcaagat gattattaaa
    25861 aagtaaagga atgatttcaa tgaaagtcgt tttccatatt gatgaattag aaaaatggtc
    25921 agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct ccagaaacga cgattgtcgt
    25981 atctgtaaat ggcatcgcga ttaccggtta tctagactct gccaatgcag aattcttaga
    26041 tttgcaagga gtcgtttttc acgcttgcgc caatgctatg cgtgctaatc atatttcaga
    26101 aagttcttta cctgaacaag taatcgttgt accagcaggt gtccttgatt tagttgaact
    26161 gcaatcccaa ggttatgctt atatcaagcc ttagtcagta gaggttgcga cagaagtgat
    26221 cagcttcaag aaatttcggc ttatttttcg aagaagctac ttctggaaaa ccgtgaatca
    26281 aaggttgtga aaa
//
LOCUS       DACEKG010000037        25945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEKG010000037 DACEKG010000000
VERSION     DACEKG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.037"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(87..2354)
                     /gene="gshAB"
                     /locus_tag="IW664_001475"
     CDS             complement(87..2354)
                     /gene="gshAB"
                     /locus_tag="IW664_001475"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0848842.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2541..3539)
                     /gene="ftsY"
                     /locus_tag="IW664_001476"
     CDS             complement(2541..3539)
                     /gene="ftsY"
                     /locus_tag="IW664_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0848843.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3551..4360)
                     /locus_tag="IW664_001477"
     CDS             complement(3551..4360)
                     /locus_tag="IW664_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0848844.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4369..7950)
                     /gene="smc"
                     /locus_tag="IW664_001478"
     CDS             complement(4369..7950)
                     /gene="smc"
                     /locus_tag="IW664_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0848845.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7970..8656)
                     /locus_tag="IW664_001479"
     CDS             complement(7970..8656)
                     /locus_tag="IW664_001479"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0848846.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9004..10785)
                     /locus_tag="IW664_001480"
     CDS             complement(9004..10785)
                     /locus_tag="IW664_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0848847.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10811..11725)
                     /locus_tag="IW664_001481"
     CDS             complement(10811..11725)
                     /locus_tag="IW664_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848848.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11752..12714)
                     /locus_tag="IW664_001482"
     CDS             complement(11752..12714)
                     /locus_tag="IW664_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848849.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12714..13658)
                     /locus_tag="IW664_001483"
     CDS             complement(12714..13658)
                     /locus_tag="IW664_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848850.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13659..14663)
                     /locus_tag="IW664_001484"
     CDS             complement(13659..14663)
                     /locus_tag="IW664_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848851.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14931..15173)
                     /gene="acpP"
                     /locus_tag="IW664_001485"
     CDS             complement(14931..15173)
                     /gene="acpP"
                     /locus_tag="IW664_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0848852.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15295..16296)
                     /gene="plsX"
                     /locus_tag="IW664_001486"
     CDS             complement(15295..16296)
                     /gene="plsX"
                     /locus_tag="IW664_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0848853.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16345..18381)
                     /gene="recG"
                     /locus_tag="IW664_001487"
     CDS             complement(16345..18381)
                     /gene="recG"
                     /locus_tag="IW664_001487"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0848854.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18498..20180)
                     /locus_tag="IW664_001488"
     CDS             complement(18498..20180)
                     /locus_tag="IW664_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0848855.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20232..20594)
                     /locus_tag="IW664_001489"
     CDS             complement(20232..20594)
                     /locus_tag="IW664_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0848856.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20864..21052
                     /locus_tag="IW664_001490"
     CDS             20864..21052
                     /locus_tag="IW664_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0848857.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21290..23959
                     /locus_tag="IW664_001491"
     CDS             21290..23959
                     /locus_tag="IW664_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0848858.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24050..24670)
                     /locus_tag="IW664_001492"
     CDS             complement(24050..24670)
                     /locus_tag="IW664_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0848859.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24782..25183
                     /locus_tag="IW664_001493"
     CDS             24782..25183
                     /locus_tag="IW664_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848860.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25318..25656
                     /locus_tag="IW664_001494"
     CDS             25318..25656
                     /locus_tag="IW664_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ0848861.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7027 a         5500 c         4644 g         8774 t
ORIGIN      
        1 acgaaaaaac acataaattt ttacatgcaa aaaagctttt gatcagattg acatcaaata
       61 ggacaatctg tcaaaagctt tttcttttaa aatacttcag gatataacaa tttcaataca
      121 ttcatcgtca atcttctgcc ttttcctgca aagggataaa catgcatatg catggctgga
      181 ttgaaatttg cttcgatgat cccatatgct tttttatcag ttgtaggatc gatttcttta
      241 tctggtataa tcaaatcgat accactaatc ttagctccta gtgcttctac cgcagaaacg
      301 gcgatttttt tgtaagcttc agaaaattca tcagtcatat cgatcgaatc accacctgta
      361 ctgatatttg agttctccct tagataaaca atttgattgg cttggggaac tgattcgatc
      421 gtcaaccctt gttctttcaa catcaattgt tctaattcac ctaattggat caattctaaa
      481 ggtgcacgat gattcgttcc acgcaaaggg tcactatttt tttcttcaac aagttctttc
      541 actgttcgga taccgtcacc aataacatta gcagggactc gcaacatgat ggcttggact
      601 tctccgtcga tcacaaagaa acgatattcc gttcccggca taaattcttc tactaaaacc
      661 gaactgtctt cacgaaaagc aatggctaaa cctgcttgat aatcttccag actagctcct
      721 tccttgaaaa tcgtgatacc taacccgtaa ttggttgatt taggtttgat cacaaatgcc
      781 tgctcggcaa atctcggata agcttttacc gcttcttcca tggatgaaaa ttctgcgcct
      841 cctggtaccc ggaacccagc ttcttttaag acttttttcg ttactgtttt gttttccatg
      901 ataagtggga cgatatagct gtccttactt gtcatgtttg catttttcac atattctaca
      961 tggtcttgga attgcagacg caaaaattga tccgactcat ctaatacttt gacttggaca
     1021 cctttttgga tggcatcgaa cataaatatc tgagtagaaa gttccatttc gcgaaaaccg
     1081 gccaactgat agggacgttc atggctttct ttataatatt gatgtcccaa tgccacagca
     1141 acttcttgct gactcgatag ctgtgcttct tgatagagtt tcgctgctaa cgtttgttca
     1201 ggatgatcca tttgagtcaa tgcttcctct actaaataaa gagaatccaa ccaatccaat
     1261 tcaaccagca tttgcttcat cccttcaaga atttctctac cttcttgatg aaattctgtt
     1321 tgatctgatg gttgttctag tgctactttg ttatttcgag tcgtaccttc tgctacccat
     1381 tggtcacagt cttctttttc ttctgtccat aacatgtaca tcaggaataa ttgtaagaac
     1441 cgaacttgct ctggattgat acccaatctt gcataaggat tcaaatcgat attccgcaat
     1501 tcaatataac gaacacctgt tgttgctaaa tctgcgactt tctttccgcc tcggaaacga
     1561 agcggtgtat aaaattcttt ctcctccgaa agtttccctt cttcgatcat tcgctcgata
     1621 tctgctaaat actgtttcat agaagcatag gaaactttca catttggatg attcgtatag
     1681 cccagcgcac tgttacgaat actgcgaaca ggttcttgcg gatgtgattc atctaaaaag
     1741 tagttcttct cactcattgg tgaagcaccg aataaataag tgatcatcca gcgatagcgc
     1801 ataaagtttc tcgccgtttt caaataaagt tctgttttga attcggaaaa atcttgaaat
     1861 tcttcctgat ggctgaacaa tgttcttagc aactcatcgc caaattcaaa attgaaatgg
     1921 ataccgctta ccatctgttt tcgcttcccg tattcttttg ctaaataacg tcgatacaag
     1981 acatcttcga aattttttaa ttttgcaata atgatcgctt cgtctttttc cggtaaggca
     2041 ggtggcatac ttaatggcca gatcatctct tctttcggta tagaacgagc agtcacatca
     2101 taaacagcag cgagatactg aaataattcg ggaatagaat cggtaacagg agtgatcatc
     2161 tctgtttgtg tttcgctaaa atctgtttgg atataggggt gataggatcg atcgccaaag
     2221 attgctggat gatcggtttt tgctagattt cctgcaagat caacccgctg tccttcccgt
     2281 tctataccaa atcgagcttg gtcgataaaa gggcgcgcat tgacatgcaa taataattgc
     2341 ttaaaattca tcattaagtg acctccaatt catatgaatc taattataat caaaaagtct
     2401 aagaaaaaga acgaaattgt ttgagaaccg cttacaaata agtataaaaa tgaaggcatc
     2461 aaaaacacag ctatgttaaa aacaatcatt caaacaagga gctgcaactt ttgtcacagc
     2521 tcctgctttt tagacatggg ttattcttct tttagtaatc ctttgaacaa tccaacgaca
     2581 aaatcatttg gatcaaacgg ctccaagtca tcgattcctt caccaagacc gaccaatttc
     2641 actggtaaat gcaattcatt acggatagca agtacgatac cgccctttgc tgtaccatct
     2701 aattttgtca aaacaagacc ggtcacatct gttgtttctt tgaattgttt tgcttgaacc
     2761 atcgcgtttt gaccagttgt tgcatctagt acaagaagtg tttcttgggg tgcttctggt
     2821 aattcccgct ggattactcg ttttattttt tctaattcgt tcatcaagtt cactttattt
     2881 tgcagtcggc ctgcagtatc aactagtaaa acatctgcct gctctatctt tgctctttcc
     2941 aaagcatcga atacgacagc tgctggatcg ccgcctgcat tgccacgaac aacttcaaca
     3001 ccagcgcgtt cgccccagac aactaactgg tcgattgctc cagcgcggaa ggtatctgct
     3061 gctgccatca atactttttt accttcgttt ttgaactgat gggcaagttt accaatactt
     3121 gttgttttcc ctacaccatt aacaccgaca aacaaaataa ctgttagtcc gtcttcttgg
     3181 atatttagtt catttacttc attgattcct tccgcttcat agagatccac aagtttctcg
     3241 atgatcgcat tttggacttc tgccgatttt ttcgcatttc tgagtttcac ttcttgacgt
     3301 aatgcttcgg tgattcgtat cgctgtttca aaacctacat ctgcaccgat aagtgtttct
     3361 tctaattctt caaaaaagtc ttcatcgact gttcgaaaat tggcaaacaa ctcattcatc
     3421 cgttcaccaa atgttttacg cgtcttttct aaacccttgt cgtacttctc ctgtacttca
     3481 acttcttctg ttttctcttc gccagtaaaa gcacgcttga ttttatcaaa aaatcccatt
     3541 tgactccccc ctactctaat acaaattttt ggatcacttt ggctacgcca ttttcttcat
     3601 tgctggctgt cacgaaatct gctgcttctt tgacgaaagg cacagcattc tccatcgcta
     3661 cgcctagtcc agcgtattgg atcatcggta aatcattttc ttcatcaccg attgccatga
     3721 tttcttcttg tttcaaacca agatcttttg ccaataaaga aataccataa gcttttgtaa
     3781 caccttttgg cataaattcc aaaagcatac ttctcgtttt gatgatctca tacatttcat
     3841 ggaattctgc tggtatttct ttgattttag gatctaattc ttcttggtca taagcgatta
     3901 ctgccttatt aagcacaaat tcatctgtaa tatcgcttat atccgctggt tggaattgca
     3961 gcagcttatt caacacattg tacaaggatt gcttccttgg ggcactaggc agctgtaaaa
     4021 ccacattgtc agacaaaaca tctagtggca gatccaattg ctgagccagt ttgtataagc
     4081 gttggatatc tgatacttcc atcactttct tttccatgat ttcaccagta tcattttttt
     4141 gtaccaaacc gccgttgaaa gtgatgctgt agtcaccttc atctaccagt cctaattctt
     4201 gcagataatg caccatcgca gctaaggggc gtcctgtaca tagaacgatc ttgacccctt
     4261 gctgttttgc ttgtgctaat gcttgtttat tctcatctga aatcagcttt tgttcattta
     4321 ataatgtacc gtccaaatcg attgctacta atttgatcat tttcgttcct acactttcgt
     4381 ttcgttgatt ttcgcctctt cattgatatc ttccatccgt acagaaacga tttttgatac
     4441 accggattcc tgcatcgtca ctccgtacag tacatctgct gcgaccatcg ttcctttgcg
     4501 gtgtgtgact acgataaatt gtgtgtcatc ttggaaatcg ctcaagtaac gaccaaaacg
     4561 tttcacgttg gcttcatcta acgctgcttc tacctcatct aagatacaaa acggaacagg
     4621 acagacacgg ataatagaaa agagcaacgc gatggctgta agtgctcgtt ctcctccaga
     4681 aagcaaactt aaactttgca gttttttgcc aggaggttgt acttcaatct caatacctgt
     4741 tttcagcaaa tcagttggtt ctgtcagtac taattcagct ttaccgccgc caaacatatt
     4801 tggaaagaca attttgaatt cttgacggat agcttcaaat acttctttga atcttgtccg
     4861 cacttcatca tccatctcat ccatcgtttc aaacaattgg tttttcgctg ctaacagatc
     4921 atctcgctgt gttgctaaga atgtatgtct ttcactgact tgttcgtatt gttcgattga
     4981 atttaagttt actggtccca gtttttcaat ctgttctttt aaggatgaaa cctttgttct
     5041 actgtcttcg atatcggttg tttcttgata gtcttgggaa gctttctcaa atgtcagctg
     5101 atattctgac tgcagatagg tcaaatgatt atctaaaatc atttcagctc ggtctttttg
     5161 gacttctaat ttggattgtt ccgacagtcg tgctttttgc tcacgattac gctctgccaa
     5221 gactgcttca agcttatcga tctccttttg ccttctatct cgctgctctt tggcttttgc
     5281 caactcggct ttcaatgttt ctcgctgagc agataattcg ttaatttgtt tttccaaact
     5341 ttcttctgtg acttcatgat cagaaaagtc tgctgtcaat gtcgccaact gcttttctat
     5401 cgcttcttga cgttcggttg cctctgcttt ttggactcgg gcaccgcgga gctggatctg
     5461 cagatgatta aactgctctt tcaagacagc aagatctgct tgttcttgtg ctttttgact
     5521 ttgtacttgt gctcttctgg cttccatctg gtcgctttct tgacttaacg atttgatttc
     5581 ttcgtcgatt ttttgtcgtt gcgattcaag atctgtttgt ttgtctttca attcgttcgt
     5641 ttgcttttcg taatcttcga taaactgctg cagttcacgt gtctcaaaag ttgagatttg
     5701 cttttctttt tcaaaacgtt caagttcatt tatgatgttt tgcagctgat ttgtcgcttc
     5761 ttgttcttca aaacgtaatt gttcgcctcg tgtacgcaaa acttcttgtt cttcggctaa
     5821 acgagctgtt tcttgttgca gctcctgaac ttttttttct tgtgcttgga gttgtttatc
     5881 tgcttcttca aattcactag tcaactgttt caattcttga ttttgtacaa acaaactgcc
     5941 ttggtttcct cgtttattcg ctccacctgt catggatcct ccggcattca tcacatcacc
     6001 ttctaaggaa accactcgat attgatagcg gatcgtttgc gcgattgcat tggcactagt
     6061 taagtctttg gctaaaagga tcgttcctag caggttttga accacagttt ggatttgatc
     6121 gggataactg acttgttcac tggcaatccc tataaaccct tctacagctg ctgcttgtgt
     6181 caatatatga gccggtaatt gtcttggttt gattgtcgtc agtggaagga atgttgctct
     6241 tcctcctctt tgctgtttca aatacgtgat ggcttgccga gcatcttttt cattttcaac
     6301 gatgacatgc tgggctgccc cacctaaagc tgtttcgata gctaacgtga aatctgcagg
     6361 aacatcgatc agttcagcca ctgctccgac aatacccgaa agctgctgtt tatgctgcaa
     6421 gaccaatcga accccttggt aaaaaccaaa atagttttct tggatttctt gaaggctttt
     6481 cttacgagca cgtagctgct ggacttgatt cattaattga tacattttag gctgttcttt
     6541 tgctaatgct tcttggaact ttttcccgtt ttgttggatc atttctaact tctcttttgt
     6601 ttcagttaaa gatgatttta gctgcgcctg cttttgtgtc aattcttctt tttgtaagat
     6661 caatcggtct actgatgcct ctacttccga ttgcttggct aacgtctgct tactttttgc
     6721 tgtttcttgt acatattgac gttctagata cttcagttcg tttcctacag ctgcttgctc
     6781 ttgcatcaaa tcaacatatt gatctcttag ttctgccagt aattctttcg ttgatttttg
     6841 gtatttttct aattcttgct gtgtttttat gagacttgct tcagcttttt ggatttctgt
     6901 ttctttttct gctacttctt tcaacaagct ttcttgaagc ttttcaaaat gtttaacctt
     6961 tttttgtgct tctgctaatg aagtttgata ttcttgcgag cttttttgtg tatgctttgt
     7021 ccgctcctgc aaaacatctt tttgtccttc tgtctgcttc agtttttccg aaagatctaa
     7081 taatacttgc tgattttttt cgatcagacg atcggcttgc gcattttctt tacgctgctt
     7141 cgccaaaatg gattcttgtt cttggatcga ctcagatagt ttgcctaatt ctagattgaa
     7201 tttcgcaagc tgtgcttgtt tgttgtccca atcttttttt gctgtcttga tttcggcaac
     7261 catcaaagag acatcggttt gagtcagtgt ttcttttagc cgtaaaaact cctttgctgc
     7321 ctcactctgg gcggccaaag gagttaactg ttcttccaat tcgtggatga tgtcttggac
     7381 gcggctgagg ttgtcttctg tttcaaaaag cttctgttct gctttctttt tccgctgctt
     7441 gtatttcaag acaccagctg cttcttcaaa gatcccacga cggtcttccg gtttactact
     7501 gaaaatcgct tctacttttc cttgagaaat gattgagaac gattcttttc ctaatccaga
     7561 gtctaagaac aattcttgga tatcttttaa tctgcagctt tgtttattga tgaaaaactc
     7621 gctttcccca gtccgtcgat aacggcgggt tacactgatc tcattgtact ccaatggcag
     7681 ataatgatca ctattgtcta aaatcactgt gacttctgca atattcagct gcttacgggt
     7741 atcggaaccg gcaaagatga tatccggcat ttttccgcct cgcagacttt tagcagattg
     7801 ttctcctaat acccagcgga tagcttctgt aatattgctt tttccactgc catttggtcc
     7861 aaccactgct gtgacgctat tttcaaagtc aatgactgtc ttgtctgcaa atgatttaaa
     7921 accggctatt tcaattcgtt tcaaatacaa agctttccct cacttacttt tacagtgctg
     7981 ccaatgcatt ttccgccgca gcttgttctg ctaatttttt cgattttcct tgtccagtgc
     8041 caatcaattg atcatctaca taaacttcga tccaaaatac tcgttcatga gctggacctt
     8101 cttcattgat caatcggtat tcgattgata catcacctga tttttgcaag acttcttgta
     8161 gttttgtttt atgatccatc tcatgtgaaa aagcacctgc tttgacttta ggaaaaatca
     8221 ctttttcgat gaatgcatgg gtcgtgtcga atccctggtc aagatataac gcgcctaaaa
     8281 aagcttcgaa taggtcgcaa agcaatgctg gtcgcgtacg tcctcctgaa ttctcttctc
     8341 ccttgcccaa caaaacatat tgatcaaagt gacattcttt ggcaaatttc gacaagctgt
     8401 cttcgcgaac gatcgcagct cgtgtttttg tcaattttcc ttctggcaaa tgaggatata
     8461 aacggaataa atactgagaa accatcagtt ctaaaacggc atctcctaaa aattcaagtc
     8521 gctcattatc cgataattgc agattgcgat gctcattcac ataggatgaa tgggtaaaag
     8581 cttgttcaag caaatttaga tcgtgaaaaa cgatgttata gttttctttt agttcttttg
     8641 tcagctggtt gtccattttc cacattcctt ttatggatat tttttcgata cttccttatt
     8701 atactggttt tttgtcaaat ttgaaatggt tcggtcaaaa ggtaagtaaa gctttaataa
     8761 tcctagtgat tgacgagaag ttgtgaaaaa ttcgtttagt ttcgagtatg aagaaaaaat
     8821 tttgtgaaaa tggctttcca tatttttgca taaatttgac ttaataccga gaaactggac
     8881 tttgaacacc gtttattgga gaagtcatga aatcagcgcc ttgacctgaa tattagagaa
     8941 ataaacagaa aaatatcaaa aaaccgaaaa aggacatcgc cctttttcgg tcatttttag
     9001 tgattatgaa cgtgaatctg ctgtaacaga aacatttgcc catgggtttg gtacatcata
     9061 agaccaatcc cagtctttga cacggtcgtt gactggcaat acttcattac ggaacaacgt
     9121 tggaatcgca aatgcttctt caaatgcata ttgttgccat ttttgatatg cttctttttg
     9181 gtactcttca tcaaatgctt tgcttgaact gatatcgttg atcaattttg tattttcttc
     9241 tgattcataa cgagtgtagt tgaacgcagc atttggtccc catagtccac ttggatttgg
     9301 atcagttcct gtattccaag cagcttggta aacatcaatt tcaggatcat cattttctaa
     9361 tttatcatag aaagaattga attcgatcag acgacctgtt gtgtattgga catccaaacc
     9421 gatttctttc cactgttgga tatagtaatc tgccaatggt tgcgctgttt ccccaccgga
     9481 catcgaagca aagttgattg ttagtttatt tccatctttg tcttctctta aaccatcgtc
     9541 atccacatct ttgtagccag catcatctaa tagttttttc gctttgtcta gatcttgtgt
     9601 gaatcctttc atctcatcat catggtatga ctggaatact ggaggaatca atgaagtcgc
     9661 attgcttctc aaacctgcat agaaacgctc acctactgca ttattatcta atgcatagcc
     9721 cattgcttga cgtaatgatt tatcagccat ttttgaatct ggattgtatt ccacactctt
     9781 tgtatctgca ttccatttac ctaacttgaa tcctagataa gtataagaag tttgttcacg
     9841 gcctaagttt gtatagccag caatttcttt ataggtatcg taagtatctg ttggcatttg
     9901 gaaaatcata tcatataatt tagagttcaa tgcttcagtt gcacttgcca tcggtacaga
     9961 tttcatcgtg atctttttca atttaggcgt accattccag tagtgctcgt ttggcaagta
    10021 ttctactgat tctcctgcaa cgatattact catgtaataa gggccaaaag tcactggatt
    10081 tttacgtact tgatcgcttg attccatatc tttgattgct acaccttcta acgtatgttt
    10141 tggtgctgca tatgaccaaa taccaccacc cgattgaagc atgcttgggt ttacttcttt
    10201 atattcgatt tcaactgttt tgtcgtctat ttttttgatt ccggagattg tgtcggcact
    10261 tccatcatga tattcttcca tacctacgat gttacggaaa gtgtcgtcat aacggatacc
    10321 agtgtaatct ttattcccaa tgatttcgta agggaaaatc acatcgtccg ccgttacatc
    10381 ttcaccatct gaccatttga cgttgtcttt tactttgatt gtcgcttttt tcgtatcttc
    10441 atctagatca agcgttgcag ctccgtcgtc tgtgattctg aaatcgtcat cgtttgcaaa
    10501 tagcggttca tgagaaggag ccataaaagc tgcatcatat gaatcttggt aaaactccca
    10561 ctggaacaat cctttaaact gactgtccgt tgcgaccgca gcttctaatt cgccgtcttc
    10621 gattgcatca tcattatttt ttgttgcaat tggaaaagtt aattcctctt tgttattgcc
    10681 gttgcctcca gagttgtttc cagaggtatc tgtacctccg cccccgcatg ctgcaagaac
    10741 gacagcagac atcagcgctg caaatccaaa aatcgttttc ttcattccta tttccccctt
    10801 cgcttctata ctagcctaac cgttggcgcg catctgcaga acgcttgaat gcttgtccaa
    10861 cataattgat actcaacatc aaaactaaaa taaggattga tgcgggtaac cagatccatt
    10921 gccgattcac taagacttct ccgttacttg cataaccgat caatgtacct aaactaggta
    10981 catttgccgg caagccgaaa cctaagaaag tcaaagttgt ctcgataccg atgtttgccg
    11041 caaagttcat cgtcaaatta gtaatgatta atgaacttag attcggcatg atttcgcgga
    11101 acatgatttt aaaatcattg gttcccattg tttttgaggc actgacataa tctcttcttg
    11161 cttcagataa tgtcttgctt cggaataatc gggctttagc tacccaataa aatgcactca
    11221 tgatgaatat aaaggaccag acattgtatc tggaaataat cgatacgaag acgataatga
    11281 tcagcatgat cggtagaatc atgataaaat caacgatccg catcaagatg ttgtcaatga
    11341 tccctccgta gtagccagag atgatcccta atccaacccc gatgatcgaa gtaaggatcg
    11401 tgatcgcaaa tccgatcgta acagagtttc tagcacctat gatcaattgt cctaatacat
    11461 cacgtccgcc ttcatctgct cccagaatga acttttgtcc ttgtgtcgaa actgttcctg
    11521 gtgcagcgta tttgtcgaag atactaacat acatcacttt gtcttgatcg gtcagcatcg
    11581 caccgataaa gatcactaat aataaaacca ctagcaaacc taatgaaaaa atagcaattt
    11641 tatctttttt aaattctcga gcaatcattc gtatccccat aggaggaata ctttcttgaa
    11701 ctgctgtatt gtttttttca ctcatcgatc cattctcctt tctgattggt tttattgaat
    11761 ccgaatacgc gggtcaacga tactcatgat gatatctgaa agtaatgtcc ccactagcgt
    11821 cgcgattcct aaaatcaata ctaaggatgt gatcacggta tagtcccgtt ggccgatgga
    11881 atcaataaat agttttccga ctcccggata accaaaaatc ttttcgataa ctacagaacc
    11941 agcaatcaac gaagtgattt cataccccat ttgagaagcc atcggcaacg ctgcatttcg
    12001 gaaaatatgt cgactgaata ctttattcgt cggtacccct ttggaacggg ctgttctgac
    12061 aaaatcgagc gatttcgaat cgattacttc acttcgcaga tattggattg tgacggcagt
    12121 cgccaaaaat gcttgggtaa ttgatggtaa cagaatatat ttcaaacggt ctatccattg
    12181 agtgataaat ccaccttgca aacctagtgt ttgtgatcca cttgttggga accagcctaa
    12241 acgataacca aaaacaaata gcatgatcaa gccgaagata aataaaggaa cagcaaaact
    12301 aaagaagttg tagattacga caatcttatc caagattgaa ttttgatacc ggccagcaag
    12361 catccccaga ggcacagcaa tcaaataagt aaggacaact gtcaagatcg atagatataa
    12421 agtgttgatg atccgttcgc cgatcaagtc agcaacagag cgtttgtata agaaactttg
    12481 accaaaatcg ccttgcaaag catttccgat ccaacggaag tactggacat accatgggtc
    12541 attcaagcca gctgcttgtc gcatctgttc gatcactgcc ggatcctgat ttggattgat
    12601 caaaccggta aatggatccc ccggcatcgc ttgggctaat aagaagatca gaacacttaa
    12661 aataataact tgcgggatca tcaataaaac acgtcgcaga atcgttttcc acattagctt
    12721 gcacctccat ctttcaacgc cactttgtgt gtagttgtga tcgtacgcaa atcgtagact
    12781 cgaccagtcg catcatagta ttctttttgg ttttggatat actcttgttc tactcttcgt
    12841 cgattttctt tgtggctttc cctattttct acatcgattt gcggaatagc tgaaagcaga
    12901 cgtttcgtat agatatgtcg cgggtcatta taaatgtctt ctcttgtacc gatttctaca
    12961 aatcgacctt tgtacataat tgcgatattg tcgcacatgt gttttactac acctaaatca
    13021 tgtgaaatga ataaatagct gaggccgtat tcttgctgga ttcgtttcat gaaattcaat
    13081 acttgcgctt gaacagatag atccagagca gatacaggtt catcggctac gatcagtttt
    13141 ggattggtag caacagcacg ggctactcct agacgttgac gttgtccgcc tgaaaattca
    13201 tgaggatatt tatatagcgc atcttccggc atcccaacga tatctagcag tccttttacc
    13261 tttttctttt cttcttgatc gcttaaccgt tcaaaatttc ggatgggttc agcaatgata
    13321 tccaacactc gctttttagg attcaaactg gacattgaat cttggaagat catctgcaca
    13381 tccttgttat aacccatttt tttgcggttg cttcgcttcg ttacatcttt tccttcatac
    13441 atgatttgtc cggctgtcac cttttctaat cccacaacgg cttttcccgt agtagatttt
    13501 ccagagccag attctcccac taaaccatat gtctttcctt tttcgataat aaaatccaca
    13561 ccatctacag catatacatg atccgttact cgattgaaga atccactgcg aatagggtaa
    13621 tgaactttca ggtctttgat tgtgattaat tctgtcatct acacctctcc tttttcgtct
    13681 ctaaagtgga aatgcttata gcaggagcaa cgtaccaagt ggttcggtgc gacttcatga
    13741 aggataggct cttcttcatg atcgcttgct ggtatccatg gaatacgtgg tgcaaaccga
    13801 caacctttac gaggcatgtt tttcagagaa ggaacgacac cttcgatcac atggagttct
    13861 gcatcatgat ttccttcttg tggtatagag ttcagtaaag agcgtgtata aggatgctta
    13921 ggatgatcaa acagttcttt tacacctgct acttcaacga actgtccgcc atacatcact
    13981 gccactcgat cagccatttc tgctacgact cctaagtcat gagtgatcaa gatgatccct
    14041 gtttttgttt cttcctgaag gtctttcagt aaatcaagaa tctgtgcttg gatggttaca
    14101 tctagggctg tcgtcggttc atctgcaatg acgattggag gtttgcaggc gattgcgatt
    14161 gcgatgatga cacgttgtct cataccgcct gatagttcat ggggatattg gcgtccaact
    14221 ctcgcaggat taggaatccc tacttgttct agcaattcta gtgcacgagc ttgacgctgt
    14281 tctgcattca tctttgtgtg ataggaaagt ccttctttga tctggtcttc gattctcatg
    14341 agaggattta atgcagacaa cggatcttgg aaaatcattc cgatatcatt tccgcggatt
    14401 ttattataga gcgtttcgtt caggtcgatt aaattcagat cgttatataa aatctcacct
    14461 gtaatttttg tgttattagg atcaagtaat cccataatcg tagtagccaa ggtacttttc
    14521 ccgcacccag attccccaac gattgctaaa atctcgtttt tgtctaaagt aaatgaaacg
    14581 tcatctactg catcgtaata gtcatctttg agtcggaatc ctgtatgtag gttctgaact
    14641 tctaataatt ggttatcgga caatcgaata cccctctctg cttcctaaat ggaagttttt
    14701 cttaataaaa aaggacatta cacagaataa agcgttttat aacaaaggtt attcttatct
    14761 ttgtgtaatg aattattaat aattatatag aaaatcgctt tactttgcaa cataatttct
    14821 gtttatcgcc agaattatca gaaaaatgac ttttacgtgt caaaaagatt aaaaaaagaa
    14881 ggataaaaag ctgtatgcag ctttttatcc ttctttgctt ttatttgaaa ttatttaaga
    14941 tggctgctga tataatctac tgcgccaccg actgtttcaa tttgttcagc gtcttcatct
    15001 gaaatttccg taccgaattc atcttcaagc tctaaaacga attccataat actaattgaa
    15061 tcagctttta aatcatcttt gatgttcaat tcatccgtca ctttatcggt atctatctca
    15121 aaatgatttg aaataatttt cgctactttg ttaaatactt cttcgcgagt caactttttc
    15181 acctccaaga atcatacact tgtcaattac ctacaccatg gctaatttta ctggcatttt
    15241 tcaggaatgt ccaccttttt ttcaatggaa cggattgctt cagatatcca aatactattc
    15301 ttcgcttttt tcaaattgtt cgactaattg tccaactaca tctgtctcaa gcattgtgtg
    15361 gatttggcgg atcgtatagc gaacagcatc ggggcctgtt gctccatgag ttttaatgac
    15421 tggtgctttc aagccaaaca aaacggcacc gccatgtttg gaataatcca tttctgcttt
    15481 caaagaacgt aagccgtctt ttaaaagcat agcgcccatt tttcctttga tcccttcatt
    15541 tagaatagca gattttaata gattcatcat attcattgct gttccttcaa tagatttcaa
    15601 aacagcgttg cctgtaaatc catctgtgac cactacatca gccacaccat tgagtagctc
    15661 acgagcttct acgtttccaa taaaattcaa gcttgtttca ttctgaagga gttcataggc
    15721 ttttttcgtc acttcactac cttttgttgc ttctgttccg ttgtttaaga gtgcgacacg
    15781 aggacgtccg atacctcgga ctttacgtgc ataaaaagaa cctaaaatac cgtattgcaa
    15841 caaatgttct ggtttgtttt ctgcgttagc gccaagatcc agcatatcga aaccggcacc
    15901 ttctttccct actactggca aggtagacat caagccaggg cgttcgatat ttttgatacg
    15961 tcctacgatg aacaaccctg ctgctagtag cgcgcctgta ttccctgctg aaaatacagc
    16021 atctgcttca ccgtttttca ctgcttgtgc agcaaggacc attgatgcag tcttttttcg
    16081 tcgaattgct tttactggct catcatcact attgattttt tcgtctgtat ggataatcgt
    16141 gatgtttttt tcatttgtta agtatttctt gatttctgct tcttttccgt aaagtaagaa
    16201 ttctacttct ggaaaatctt gctgcgctaa agccacgcct tctacaattg cttgaggtgc
    16261 gtgatctccg cccattgcat ctacggcaat tttcattgtt tccactcctt tttccgtcat
    16321 cagtataaag tatagcacag tctgctaatc gaaataacga ttatcctgat tttctatttg
    16381 gaggctttgc gctaaaggct gatactcagg aagcatctgc caatccttta tctgccaaag
    16441 ggctgcagcc tcttctctgg ctacctccaa aacatttcca tccgtcacca agtcagcaat
    16501 cgcaaattga ggcagaccag attgacggaa gccaaatact tcaccaggac cacgtaattc
    16561 cagatctttt tcgcttagta caaaaccatt ggtggtctct gtcatgattt tcatccgttc
    16621 cacacctagt tcatttttag gattagcaac taagatacag taggaagcat cacttccgcg
    16681 tccaacacgt ccacgaagtt gatgcagctg tgccaatcca aatcgatccg catccatgat
    16741 cagcatgacg gtcgcgttcg gcacgtttac ccctacttca attacagtag tcgaaactaa
    16801 tatctgcatc tggttgtctt tgaaggcttc catgattact tctttttcct gattcttcat
    16861 tttaccatgt aacaagccaa cttcatattt tggtgcaaat agttcacgaa gtttttcata
    16921 gatttccact gcatttttta catctaatgc ttctgattct tcaatcagcg gacagatgac
    16981 atacatttga tgtcctcttg ccagttcttt tttcgtccaa tccagtacac tgtccaattg
    17041 aggtgttctt acccagcgtg tttcgatcgg gatacgtcca gctggaagtt catcaatgat
    17101 agatacatcc atctcaccat atgctgtaat agctaatgtt ctaggaatcg gagtcgctgt
    17161 cataaataag acatctggtc gccagccttt ttcacgaagt actttccgct ggttcacgcc
    17221 aaaacggtgc tgctcatccg taataactag acctaaattc tgaaattcga ctccatcttg
    17281 gatcaatgca tgtgtgccga tcacgatgtt aatttcccct tgctctagtt tttcaaggag
    17341 ctcacgacgt tcttttgctt tcgttgaccc cgtaagcaat gcgatggtta cttcattggg
    17401 atcaaacagc tgttgcaagc tttccatatg ttgttgtgcc aagatttctg taggcaccat
    17461 caacgcacct tggaaacctg ctgtaacggt agcgtataac gcaatcgcgg caaccactgt
    17521 ttttccgcta cctacatctc cctgcaaaag tcgctgcata tgctgcggac tgcgaagatc
    17581 ccgacagatt tcattcgtta cacgtttttg tgcatttgtc agttcaaaag gtagcttttg
    17641 agtaaattct ttcaattttt ggacatcata aaggatctcc aaaccattcg tttctgcttt
    17701 ttctgctttt ttcaatcctt gaagacgcat ttgaaataaa aagaactctt caaaaacgac
    17761 acgtcgtttg gcttgatggt tttcttcgtg gtcttttgga aaatgcatac ttcttacagc
    17821 agaagctcgg tcaagcagtc gatatttctc cacaaggttg tttggcagat tttcttcaat
    17881 caatgtgccg tattcttgaa accctttttt gattagatca actaatgtcg tttgacgaat
    17941 tgatttgttg acgtgatata tcggcgaaaa gtcatccatt gtttgagaac ctaaaatctt
    18001 catcccgttc aatgctttac gctttgcatc ccatttaccg tatacggcga tttcctccga
    18061 taaaatgact ttttctttta gataaggttg attaaaaaaa gaaacgttga acacatcatg
    18121 ttcttgcatc atacgaaatt gcaatcggct ttttttataa ccaaatcgat tcatcacagg
    18181 aggcgagact acgattcctt tcaaagtaac cttttcttga tcttggatct cgtttagatt
    18241 tctttcctga atatcttcgt agcggaaagg ataataagtc agtaagtctt caatcgtttg
    18301 gatgcccaag ctggcaagac tagctgctct tttttctccg actcctgcca gcatagtgat
    18361 tggatcatct aaagaacgca taaatttttc ctccctccct taacaaaaaa aggacttggt
    18421 cagcgatcat acctgatcac taaccgaagg ccttttatga atgaatactg tgttagaaag
    18481 atattctgac acagaaatta ttcagcagaa aatagataag gatagacagg ctgatctcct
    18541 tggtggatct cgatttccaa atcaggatgt ttttctgtta gtttctcagc aaattcattt
    18601 gcttgttcaa ctgttccttc atcacctgcc aagatcgtta cgatctctgt gtcctcattg
    18661 atcatttgtt ctagtgtact taacgctgtt gctgtgatat ctgggttaga gatcaggatt
    18721 tttccatcga tcatgcccaa aaagtctcct tctttgatct ctacaccgtc aattgaggta
    18781 tctctgatcg cgtgagtgac ttgaccgctc acaacgctag acaacatttc tgtcatcgtt
    18841 tctttgtttt cttctaaaga agcttgatca ttgaatgaaa gcatagctgt caatccttgg
    18901 gaaatcgttc ttgaaggaac aactgctact gggatatctg ctacttctgc tgcttgatca
    18961 gctgccatga agatgttctt attgtttggc aagacaatca ctttatctgc gtttacttct
    19021 tgaatcgctt taacaatgtc ttccgtactt ggattcattg tttgcccacc actgataaca
    19081 taattagcgc ctaaactttc aaacagctct tggacgcctt taccagcagc gatcgctacg
    19141 ataccatacg ggatacgtgg tgtttctttt ttcgcaaact cgttcacttc ttcatcgtgt
    19201 tccaaaatcg tctcatgctg caagcgcatg ttgtcgactt tgattttgac taatgaaccg
    19261 aatttttgtc cataattcaa gacttctcct gggttttctg tatgcacgtg gactttgatg
    19321 atctcgtcat cattgaccac aagtaaagaa tctcctatac catctaaata gttacggaat
    19381 tcttcgtaat cgaatgtact atcgactgta ggtccttctc cgatttttac catgatttct
    19441 gtacagtaac caaaatggat atcttctgtc gccatttgac cttggacact gcgatgatgt
    19501 tctgcattga ccatatcatc catttctgct ggagaaggtt catatgtttc atccgcttgg
    19561 aattctccat tcaatgcact taaaaagccc tcataaacaa acagcagtcc ttgccctcca
    19621 ctgtctacga caccgacttc ttttaggact ggaagcaagt ctggagtttt agctaaagca
    19681 cgctttgccc cttttacaac agctgtcatt acttcaacga catcatcagt actttttgct
    19741 ttcttttctc cagctttcgc tgcttctcta gctactgtca aaatcgttcc ttctactggt
    19801 ttcattactg ctttatacgc agtttcaact ccatgagtga aggctgcagc aagatcggaa
    19861 gcatttaatg agacaacatc tggaatttgc ttagaaaatc cacgaaacaa ttgagacaaa
    19921 ataacgccgg aatttccgcg cgcacccatc agcaatcctt ttgaaagcat tgctgctaat
    19981 tctcctactt tttctgaggt tgaatctact actgccttag ctccactagt catagataga
    20041 ttcatatttg ttcccgtatc gccatctggc acagggaata catttaatga attgacgtat
    20101 tctgcattta cttgcagacg attcgcacct gcctggacca tttcctggaa ctggcttgca
    20161 cttatttctg ttacactcac ctagataatc ctccttcaga tcgactgctg agttaccagc
    20221 cagtctcctg cctagtctgg cagtacacga acaccttgta caaagacatt aactgaattg
    20281 gcagttacgc ctaataatgc ctctagattg tatttcactt tttcttggac gttgcgggaa
    20341 acttcagaga ttttcgtacc atagctgata atcgtataaa catctacagc aataccatta
    20401 tcttcttggc ggacaactac tcctcttgag taattttctt tacgcaaaat ctcagtaata
    20461 ttatctttaa tttgattttt gctggccatt cctacaacac cgaacacatc tgtcgcggcg
    20521 ccaccaacaa ctgttgcaat tacatcattg gtaatttcaa tggttccagc tgacgtttga
    20581 atttttacag ccatgaatga gcctccttaa cgggtaaaaa actcccattt ttcttctatc
    20641 atattttatc atagcgcaac ttgaaataaa aggcagttac ccaaagtact caaaaacctt
    20701 gcaaaatttc aggtataacc ttgtttaatt tacaattatt cttctcaatt gtcaattgta
    20761 tctatgaaaa tgtacttgca aatttccatt ctatatgata tgttagtctg gtatgagctg
    20821 aattattcta ttgctccaaa tcaaggcaaa ggaggaaatt accatggcaa aagtttgtta
    20881 ctttacaggt cgcaagacaa aaagtggaaa caaccgttca cacgctatga actcaacaaa
    20941 acgtactgtt aaacctaact tacaaaaagt tcgcgttatg gtcgacggaa aacctaaaaa
    21001 agtttgggtg tcaactcgtg ctttgaaatc aggaaaagtt gaacgcgttt aattgaatga
    21061 tacttggacc gttcagatag tgaacggttt tttatttaat aaaaattttt aagggtggga
    21121 cttttgattc ctaccctttt tttgcgttta tttttgttat acgaacaaat tatgaagtta
    21181 tttattctaa attttatttt tttgtgaaaa ttagactctt tttaagcaga gaaaaacaag
    21241 gatatgataa actgaataca acttcataga aatttggtgg aaaataaata tgaaaaaaaa
    21301 tttaaagtca ttctttagag aaaacggacg ctacgcagca gctagtttcc tgattccgtt
    21361 tttgatttta gcacttgttt atctaagtat cgggatctat cctggaagca gcagaagtat
    21421 tttggcaagt gatgcctttt cacagttttc taattttcat gccagcttcc gaaatgttct
    21481 tcttggaaag caaagtattt tttatacatg gaatgcttct ctaggattga attatttatc
    21541 tttaatttct tactatctag gcgggatctt cacaccactg gtgttctttt ttccaaacca
    21601 gatgatgcca gatgcactgt atgttctgac tttgcttaag gtcggctgtg ccggattggc
    21661 tttttggttt cttgcacgta cgttcaagat tcctcgctgg gggcatgttg cacttagcgt
    21721 cagttacgca ttgatctcat ttatcactgc acattcggag atcatcatgt ggctggatgc
    21781 atttgtttat cttccattag tgattttggg gattcatcgt gttatggatc ttaaacggcc
    21841 gaaactttta tttatcagtt atttactctt gtttttatcc agtttttata tgggttttat
    21901 gattggcgta ttttctttct tatattttat cgcccgtcta ttgacaaact ggtcagaata
    21961 taaaaaaacg attgttcctt atggtatcac ttctttactg gcaggcggcg cttcaatgat
    22021 cattgtattg ccagcagtgc tggatcttcg gtcaaatggc gaggcactga gtcagatcac
    22081 tacgttgaaa acagaagcga cttcttatct ggatctaatc atgaaaaata tgattggtgt
    22141 ttatgacacg acaaaatacg gatcgattcc gttcgtttac gcaggtctgc taccattagt
    22201 cctctgtctg ttttattttg ccagtcggaa agtaccgttg aaaaataaat ttttgtacgg
    22261 cagtcttttc gccatactta tcgcaagttt ctatcttgtt ccattgaatt tgttctggca
    22321 tggtatgcat gcaccgaata tgtttttatt ccgttatgcc tatctgttct ctttcttagt
    22381 gctcctgctt gcaggaaaag gatgggaaaa gatagaacaa gacgatcgtg ggctgttgtt
    22441 aggaatcatt ttggtcgtag ctgctctctt tgcggtggca tggggcatca aacctgaagg
    22501 aagctacacc tatgtgacga tgacttcctt tgtattgaca gtggttttct tagggattta
    22561 tgcttttgct gtgggatttt atcagcataa aaaactttca ttcaatcatc tgagtatatt
    22621 gttattattg atcatgtctg ctgaagcact ggtcaatacg aacagtatga tccatggcat
    22681 tcttgatgat tggaattatg cttcccgcag tttatatact gatccttatc ctgacttgaa
    22741 agctcttgta aatgaaacaa aaaaagattc cgactcgttt taccgcttgg aaaatctgga
    22801 tccagtatct gcaaacgatt cgatcaacta tggttacagt ggtatcagtt tgttttcttc
    22861 tataagaaat cgtaattctt cctcttacct ggataaatta ggtttccgtt caagagggac
    22921 aaatttgaat atccgttata acaataatac tttgctaatg gatgggttta caggaatcaa
    22981 atataacatt tctaaaaacg aaagcacgtt cagtaaatat ggcttccgcc aagctggtca
    23041 aagtggcgac tatacactgt atcagaatgt caatgcattg ccactagcat ttactgcacc
    23101 gttgtccgtt aatgatgttg agcagccagt gacagataat ttgaccagtc agacaaactt
    23161 gatcaataca ttgtctggtt taaatgaaca gtattttaca ttctatacac ctactttgaa
    23221 gaatcagttg aatacgacga tcacaaagac aacgactggc gtcacttact cagaaacagc
    23281 agataaccag ccaaaagagc tcacttggga agtccatgtg cctgcaaata cacaagctta
    23341 cttaagtttg ttcccaacca attttgcaca attagaaagt tctagtgcga cgatcactgt
    23401 caatggtaca tcgcgaaaaa cgcaaatcaa catttctggt caatactatg atcttggcta
    23461 ttatcctcaa gagacaacag tgacatttac tgcaagtttt tatggaacga aagaagtaag
    23521 tttcatggag cctaaagtcg ttggtctaga tgttgtagcc tatcaagccg caatgaatca
    23581 gatcaaagaa aaaggtgtcg aaatggaagc cactggcaga aaagcttctg ggactgtaaa
    23641 taccgctgaa gattccatgt tggtgacaac tattccttat gacaaaggat ggaaagctaa
    23701 aattgatggg aaaccagtca aggtcagcaa tttcaaagac gcattcttga tgataaaagt
    23761 tccagcaggt aaacatactg tcactttcag ttacttgcct gaaggattta cgataggtgc
    23821 aatccttttt gtcctatgta ttgcattgtt tattctttat gtctggtacc ttgaccgcaa
    23881 tcgccccttt tatggcacga ttgatccagc aatagaagaa aaaccgcgaa atacacacag
    23941 aagaagacga agaaaataaa gaaacgatgg aatctgtgtc actcttctat ttaaatggca
    24001 aaaatttcaa tctttaaatg acaaaaacca cctgattttt ggtggttttt tatataccta
    24061 tgactaaact atctcttgct gcttcgacaa cttctacagt gatcttatcc atttgattga
    24121 ttcttaaaat ccgatcaatc tgcgcaaata aacgatgaat aagcctaaaa tttccttttg
    24181 tgatcttaat gatagttgtg atagcctcat aatcagaaaa atcttctagc ttgatatcaa
    24241 ttcctaaatc tgcccatttg tattccaaaa tatgatgtgt ttcgtcctta ctaaggttat
    24301 caaattcatg tgcaaatcct atgcgagagt aaagctgcgg ataacgagac aatctttttt
    24361 caataccagg cataccgata agaaccattg ccagatcatt tctatcgtaa atatctctta
    24421 gctgttctaa atgctgaagc tttaaacgat caatttcatc gacaataatt aagtcaattg
    24481 cttcataggt tttaggcaca ggaggatctt cttgattgta ttttagtacc atatgcattg
    24541 ctttagtcag acctattcta ctagttattg tgttaatatc cgaactgaca cgagacatct
    24601 tttcagctgg tacagtataa aaaactttat ttacacttaa tatttcatct gttgcattta
    24661 tcaagaacaa gaaaaagtct gaatattcaa aaaataacgg tgtacatttt taaaaggaga
    24721 tgtataatcg agatttagaa agcgttttcc taaaaattta atgaattagg aagtgattac
    24781 tatgaaaaaa ttatttagcg cactttttat tctgttattt ggctttattg gtattgggac
    24841 ttctggaaat atagttaaag ctagcacgat ggttgaacaa acaagtgaaa tggcaaaaac
    24901 ttttagtcaa ttaacaccag aagaacaaag ttacttcaaa tccaaaggat ttagtgataa
    24961 agatactttt tacactcaaa agatagagcc tcaagatccg aaaacacgtt tttctgcaaa
    25021 tgtattgcgg ttaaccgcat ctactaaaaa aattactaat acgcaggggc ggacagaata
    25081 tattataaca agtcccaatt cggcgatgta tcgtatttct acacgcttga gtttaggtag
    25141 tgcaaaatca tatactcaaa cagtaaatct agctaattct taaggttatg gtggaggaat
    25201 gtattttaca tttactggga aaagacaata ttacagtgta aaattagcta tgcaagttta
    25261 taatacattt ggtgtagggt cagtctcttg cactgcggga ggattgacaa taggaaaatg
    25321 ataatttctt actatgtctt aaatagaagt gatgatgaag gactcttcgt ttttcaatca
    25381 ttacatgagg atacgatgct aaaagagttt tataatcgga ttaagaataa acagatagaa
    25441 acaaatttat attttttaag tgactctatc ttgcctttta ttagcaaccc taaaattctt
    25501 aacttgttgc agacaaaaga tttgaaaagt ttaccgatta ttgtcattga tgaggaaatt
    25561 gtaaaatatg gcgcttatct aagcgtagaa gaattagaaa aattaactaa cattggaatg
    25621 agtattcagg aggataatgg ggtacatgga acatagaatg aatatgcaca gttgagaacg
    25681 gaatttgaca tttatttaga ccaacagggt ataccctgtt ggtctttttg tgcgttcatg
    25741 atatacttgg tgtcattttt atatttaaat ggcacaaata tttctaatta tttgtcattt
    25801 aacgctgaaa atttttgcta tttatctaaa aaagtgacag gatactcatt tggccttcat
    25861 agaattttct aaatatgaat catcaatgaa tttaattaaa aaaataagta gggcaaaata
    25921 taaatgtttt tgccttactt gtttc
//
LOCUS       DACEKG010000038        25945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEKG010000038 DACEKG010000000
VERSION     DACEKG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.038"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW664_001495"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW664_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0848862.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW664_001496"
     CDS             808..1242
                     /locus_tag="IW664_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0848863.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW664_001497"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW664_001497"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0848864.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW664_001498"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW664_001498"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0848865.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IW664_001499"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IW664_001499"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0848866.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IW664_001500"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IW664_001500"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0848867.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IW664_001501"
     CDS             9513..10439
                     /locus_tag="IW664_001501"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0848868.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IW664_001502"
     CDS             10621..11514
                     /locus_tag="IW664_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0848869.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IW664_001503"
     CDS             11648..11773
                     /locus_tag="IW664_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0848870.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IW664_001504"
     CDS             complement(11907..12539)
                     /locus_tag="IW664_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848871.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IW664_001505"
     CDS             complement(12687..13229)
                     /locus_tag="IW664_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0848872.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IW664_001506"
     CDS             13364..13753
                     /locus_tag="IW664_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848873.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IW664_001507"
     CDS             13757..14521
                     /locus_tag="IW664_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0848874.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="IW664_001508"
     CDS             14650..15597
                     /locus_tag="IW664_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0848875.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="IW664_001509"
     CDS             15727..16467
                     /locus_tag="IW664_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848876.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="IW664_001510"
     CDS             complement(16758..17426)
                     /locus_tag="IW664_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0848877.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="IW664_001511"
     CDS             17560..17847
                     /locus_tag="IW664_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848878.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="IW664_001512"
     CDS             18263..19801
                     /locus_tag="IW664_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0848879.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="IW664_001513"
     CDS             19798..20763
                     /locus_tag="IW664_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848880.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="IW664_001514"
     CDS             20750..22072
                     /locus_tag="IW664_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0848881.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="IW664_001515"
     CDS             22313..23056
                     /locus_tag="IW664_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848882.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="IW664_001516"
     CDS             23071..24063
                     /locus_tag="IW664_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848883.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="IW664_001517"
     CDS             24075..24812
                     /locus_tag="IW664_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848884.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="IW664_001518"
     CDS             24802..25524
                     /locus_tag="IW664_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0848885.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25608..>25945)
                     /locus_tag="IW664_001519"
     CDS             complement(25608..>25945)
                     /locus_tag="IW664_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0848886.1"
                     /translation="VQAEQPVVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAK
                     EWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAK
                     AFWEANGWY"
BASE COUNT         8942 a         4411 c         5403 g         7189 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
    25621 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    25681 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    25741 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    25801 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
    25861 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
    25921 acaacaggtt gttcagcttg gactg
//
LOCUS       DACEKG010000039        25675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEKG010000039 DACEKG010000000
VERSION     DACEKG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.039"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..514
                     /locus_tag="IW664_001520"
     CDS             170..514
                     /locus_tag="IW664_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0848887.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IW664_001521"
                     /pseudo
     CDS             515..934
                     /locus_tag="IW664_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IW664_001522"
     CDS             complement(1026..1376)
                     /locus_tag="IW664_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848888.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IW664_001523"
     CDS             complement(1342..2673)
                     /locus_tag="IW664_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0848889.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IW664_001524"
     CDS             2929..3495
                     /locus_tag="IW664_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0848890.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IW664_001525"
     tRNA            3640..3727
                     /locus_tag="IW664_001525"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IW664_001526"
     CDS             4061..5035
                     /locus_tag="IW664_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0848891.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IW664_001527"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IW664_001527"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0848892.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IW664_001528"
     CDS             complement(7450..7896)
                     /locus_tag="IW664_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0848893.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IW664_001529"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IW664_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0848894.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IW664_001530"
     CDS             8361..8879
                     /locus_tag="IW664_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0848895.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IW664_001531"
     CDS             complement(8956..9912)
                     /locus_tag="IW664_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0848896.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IW664_001532"
     CDS             10078..10884
                     /locus_tag="IW664_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848897.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IW664_001533"
     CDS             10881..11870
                     /locus_tag="IW664_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0848898.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IW664_001534"
     CDS             11867..12871
                     /locus_tag="IW664_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0848899.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IW664_001535"
     CDS             13003..13545
                     /locus_tag="IW664_001535"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0848900.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IW664_001536"
     CDS             13565..14263
                     /locus_tag="IW664_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0848901.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IW664_001537"
     CDS             14284..14496
                     /locus_tag="IW664_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0848902.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IW664_001538"
     CDS             14496..15458
                     /locus_tag="IW664_001538"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0848903.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IW664_001539"
     CDS             15476..15871
                     /locus_tag="IW664_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0848904.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IW664_001540"
     CDS             16034..16399
                     /locus_tag="IW664_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0848905.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IW664_001541"
     CDS             16396..18207
                     /locus_tag="IW664_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0848906.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IW664_001542"
     CDS             complement(18269..18436)
                     /locus_tag="IW664_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0848907.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IW664_001543"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IW664_001543"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0848908.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IW664_001544"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IW664_001544"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0848909.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IW664_001545"
     CDS             21193..22173
                     /locus_tag="IW664_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0848910.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22262..23086
                     /gene="tsaB"
                     /locus_tag="IW664_001546"
     CDS             22262..23086
                     /gene="tsaB"
                     /locus_tag="IW664_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0848911.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IW664_001547"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IW664_001547"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0848912.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IW664_001548"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IW664_001548"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0848913.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IW664_001549"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IW664_001549"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0848914.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4397 c         5376 g         7575 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaata aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt ttttctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggta aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctgggtgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tattt
//
LOCUS       DACEKG010000040        25378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEKG010000040 DACEKG010000000
VERSION     DACEKG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.040"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW664_001550"
     CDS             complement(3..1991)
                     /gene="treP"
                     /locus_tag="IW664_001550"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0848915.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2214..2927
                     /gene="treR"
                     /locus_tag="IW664_001551"
     CDS             2214..2927
                     /gene="treR"
                     /locus_tag="IW664_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0848916.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3004..3450
                     /gene="msrB"
                     /locus_tag="IW664_001552"
     CDS             3004..3450
                     /gene="msrB"
                     /locus_tag="IW664_001552"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0848917.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3620..4222
                     /gene="ruvA"
                     /locus_tag="IW664_001553"
     CDS             3620..4222
                     /gene="ruvA"
                     /locus_tag="IW664_001553"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0848918.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4235..5236
                     /gene="ruvB"
                     /locus_tag="IW664_001554"
     CDS             4235..5236
                     /gene="ruvB"
                     /locus_tag="IW664_001554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0848919.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5265..5843
                     /locus_tag="IW664_001555"
     CDS             5265..5843
                     /locus_tag="IW664_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0848920.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5895..6377
                     /locus_tag="IW664_001556"
     CDS             5895..6377
                     /locus_tag="IW664_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0848921.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6494..6868
                     /locus_tag="IW664_001557"
     CDS             6494..6868
                     /locus_tag="IW664_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848922.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW664_001558"
     CDS             complement(7668..9020)
                     /gene="brnQ"
                     /locus_tag="IW664_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0848923.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9167..9757
                     /locus_tag="IW664_001559"
     CDS             9167..9757
                     /locus_tag="IW664_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0848924.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9885..11234)
                     /locus_tag="IW664_001560"
     CDS             complement(9885..11234)
                     /locus_tag="IW664_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0848925.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11402..12142)
                     /locus_tag="IW664_001561"
     CDS             complement(11402..12142)
                     /locus_tag="IW664_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848926.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12155..13747)
                     /locus_tag="IW664_001562"
     CDS             complement(12155..13747)
                     /locus_tag="IW664_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0848927.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14315..14611)
                     /locus_tag="IW664_001563"
     CDS             complement(14315..14611)
                     /locus_tag="IW664_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848928.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14787..15512
                     /locus_tag="IW664_001564"
     CDS             14787..15512
                     /locus_tag="IW664_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0848929.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15505..16347
                     /locus_tag="IW664_001565"
     CDS             15505..16347
                     /locus_tag="IW664_001565"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0848930.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16350..16871
                     /locus_tag="IW664_001566"
     CDS             16350..16871
                     /locus_tag="IW664_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0848931.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17124..17687)
                     /locus_tag="IW664_001567"
     CDS             complement(17124..17687)
                     /locus_tag="IW664_001567"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0848932.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17938..18207
                     /gene="rpsO"
                     /locus_tag="IW664_001568"
     CDS             17938..18207
                     /gene="rpsO"
                     /locus_tag="IW664_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0848933.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18395..20521
                     /gene="pnp"
                     /locus_tag="IW664_001569"
     CDS             18395..20521
                     /gene="pnp"
                     /locus_tag="IW664_001569"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0848934.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            21031..21297
                     /locus_tag="IW664_001570"
     CDS             21031..21297
                     /locus_tag="IW664_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0848935.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21441..23432
                     /locus_tag="IW664_001571"
     CDS             21441..23432
                     /locus_tag="IW664_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0848936.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23786..25087
                     /locus_tag="IW664_001572"
     CDS             23786..25087
                     /locus_tag="IW664_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0848937.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8173 a         4807 c         5203 g         7195 t
ORIGIN      
        1 gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag tcctgattgg
       61 tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat gtcagttttc
      121 ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc tggtgtgaca
      181 aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac agtatctaat
      241 cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc atgttttgtt
      301 tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt tgacggttcg
      361 acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac ttcagttatt
      421 tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac tgccgtacca
      481 gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc tgtttgaagt
      541 tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat tccctgacgt
      601 ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc gatgatgaaa
      661 ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac tccgatcgcg
      721 ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc gaccattgca
      781 gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc agttacgcct
      841 aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt cccacgatgt
      901 aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc aatcatcggc
      961 cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt ggtcatatgg
     1021 tggagaccag taatcactaa aggagcatac aggaatccga atacggcact aaacaaccag
     1081 ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc gactgtccag
     1141 ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga aaacaatgga
     1201 acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc tagatacact
     1261 aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat catctggact
     1321 tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc tacactataa
     1381 gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa tatttgtgta
     1441 gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg gaaaattgct
     1501 tcacccggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg ttcaacgatt
     1561 gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc aaggatcaat
     1621 ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac ggcaatagca
     1681 cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt ggaaacccct
     1741 tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt tcctatgatg
     1801 acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc ttcgatcgct
     1861 ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc acaatgacta
     1921 actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc atctgcttga
     1981 tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt gtttcttcat
     2041 ttactatact ttttttcgga gagagttgca agcgttttat taaaaagtac gagagaaaat
     2101 gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat tgaaatacaa
     2161 aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa aacatgaata
     2221 agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa tatcctccgc
     2281 attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca cgagaaacaa
     2341 ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa caaggaaaag
     2401 gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg actagctata
     2461 aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg ttagaagaaa
     2521 cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg cctgtttggc
     2581 atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc gattatctat
     2641 taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc tatgaatatt
     2701 ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca gtagaagaag
     2761 taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca gtagtagtca
     2821 gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg gttcaccgat
     2881 tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata gttcaaaaga
     2941 aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa gggggcattt
     3001 ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt atcacccatg
     3061 gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa atatgatgat
     3121 ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt cagctctgca
     3181 gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac gaaaagacaa
     3241 gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg aagcaaagaa
     3301 gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg tggtctacgt
     3361 tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga tgaattaggt
     3421 tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg tacaatacgg
     3481 ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga aagaaacgct
     3541 ccccagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg tatgtgtgca
     3601 aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct ttatcaaccc
     3661 ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag gaaatcctta
     3721 ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg taatacgtga
     3781 agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt ttttgcgatt
     3841 agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa gcgaagacca
     3901 ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga aatttccagg
     3961 agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat tcggtgagct
     4021 gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg ctacagctct
     4081 atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa tcaaacgtgt
     4141 agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac gccaagcact
     4201 caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact tgttatcacc
     4261 agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc tgctttctca
     4321 atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag ctgccagaaa
     4381 tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag gaaaaacgac
     4441 gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga gtggccctgc
     4501 tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag gggatgtcct
     4561 gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt attcagctat
     4621 ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc cggttcattt
     4681 ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc tttctgcacc
     4741 tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac aggatctgca
     4801 ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag aaggggcatt
     4861 ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc tcaaacgggt
     4921 tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag cggatcaggc
     4981 acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga aattgttgcg
     5041 aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt cagtaaacat
     5101 aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc aaaaaggatt
     5161 tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac attttggtta
     5221 cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt gatacaacac
     5281 gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa tcttttcgaa
     5341 aaatcagcgt ccaaaaaatc gcaaatgaag caaaaatcaa tcgacaaaca ttttattatc
     5401 attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca ttgcattatt
     5461 tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa gtgctgaaag
     5521 caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca gaaattttac
     5581 aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa caaatggatg
     5641 aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt tcttacggtt
     5701 gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg atggaaatcg
     5761 ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt ctttatgcaa
     5821 aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa taatcgctaa
     5881 aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt tgccgttcac
     5941 cgatggcaga aggcttgctg agaaaacgaa tagcaataga aggaagggaa gcagaatttt
     6001 ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac cctggaacga
     6061 aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg caaatcacac
     6121 cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat gtagaagaac
     6181 tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta agtagcattc
     6241 ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt gatgagacat
     6301 atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgac gaaaataaaa
     6361 taacaaacaa tttgtaaagt tgacgctttc tttttaaggc gatgttagag catttccttg
     6421 tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct tagctaaagg
     6481 aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta cgaagttagt
     6541 tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag aacatgctaa
     6601 gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta ctgaacatag
     6661 tctagttatc ttacaagtaa gagcgaatcg tttgagtagc aagtatgaaa cgatctcagg
     6721 ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac aaaacgaaga
     6781 gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc tttcaccagc
     6841 aggggaacag gaacgattat caaaataatt tataccagat agaaaagcac gtatgagctt
     6901 gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata tcagtaaaac
     6961 ggaacgtttt aggatgagta tcacagcatg acagcattgg gttgattagt tgaatcgatt
     7021 ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct agcaggataa
     7081 ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat ataatagtca
     7141 ctagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagttttt ttgcgaaaac
     7201 tatttttata tcatatatat gtctaaaatc tatattataa aagaaatgtt gtttgatcag
     7261 cttctcttcg gtattagttc aaaacaaaac ggcaaagtat atggagctac tttgccgttt
     7321 tgtttctcta atactcaggt cagtttatag acgctcaata aacggtgttc aaaagccagt
     7381 ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag ccattttcac aaaatatttc
     7441 ttaatacttg aaactaagcg gctttttcac gacctctcaa taaacggtgt tcaaaagtca
     7501 gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc aaaaaatatt
     7561 tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt gttcaagaga
     7621 cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca atctgctact
     7681 tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac tgctggtacg
     7741 atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata taaaagattt
     7801 tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc aaaaatcgaa
     7861 gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt aaacaaagga
     7921 ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat caaaacaggc
     7981 aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg aaggatacta
     8041 gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa tgtttctgca
     8101 aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat aaatgctaat
     8161 aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt gatcccgcca
     8221 ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag caaagtataa
     8281 atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt agcgatttca
     8341 gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc aaatgctaaa
     8401 gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa agcatgctct
     8461 tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa aaaagcaagg
     8521 agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc aacataatct
     8581 aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac aatactgaaa
     8641 attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc aatctcgaaa
     8701 gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta aagcaagatg
     8761 gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac acctgagctt
     8821 ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt aaccaagaaa
     8881 cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac gggaaagata
     8941 agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag atagtcttta
     9001 aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt aacaaaattt
     9061 cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt atttaataac
     9121 gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga caaagtcgag
     9181 attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg tattagagat
     9241 caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata cattattagc
     9301 ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc gtatatttca
     9361 gatggcagaa cgaattaact accgagtggt ctggtcgaat atctttttgc tcttttggct
     9421 gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt tttggcctga
     9481 gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct tgatgaggca
     9541 aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa aggaccgtcg
     9601 aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt ttgtaccgtt
     9661 gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct ataaaaaagc
     9721 agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg acagaagctt
     9781 ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaattc
     9841 cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt cttacgttca
     9901 tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag taataaagcg
     9961 attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta aagaaatacc
    10021 agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt gccgaggata
    10081 cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa gaaacaagta
    10141 gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt caagataaca
    10201 gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg atgtgttgtt
    10261 aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag catacgtcct
    10321 gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac taaattgatc
    10381 aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg actgttatct
    10441 ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc taagtaaaat
    10501 aagatgatcc tgataggaat attattgata gcttccggca acactttttc aggattttct
    10561 gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt taccatttgg
    10621 aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga ccatagattc
    10681 gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata agctcctacg
    10741 aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc taactcacca
    10801 aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat cagttcagga
    10861 agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc tgttaaatcg
    10921 gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa agccgctttt
    10981 ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt tgataacaga
    11041 agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag ataggcgagc
    11101 aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa ccccgttcca
    11161 atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg ctgcagttct
    11221 tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt ccatattaaa
    11281 agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac cttttatgag
    11341 taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc agctattgat
    11401 cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc ggatggacaa
    11461 agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa atgacacggt
    11521 cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc aagccagtat
    11581 gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct aacgccgaag
    11641 taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca attgcgacac
    11701 gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga tccataccaa
    11761 ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt ttcaggacag
    11821 ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg ttgaaggatt
    11881 ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga gctaatacat
    11941 tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg atacagcgca
    12001 agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca cctttattga
    12061 ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt agatgtttga
    12121 tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt gaattgtcga
    12181 tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc gcccgcagga
    12241 ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta aatgtttgga
    12301 agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca gctacgccga
    12361 ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct gttgctggca
    12421 agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca tgggtcatac
    12481 caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc ccacgaacga
    12541 tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca atcgttcggt
    12601 tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct tgtaccatca
    12661 tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg attttttgga
    12721 agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca atcagtaatc
    12781 caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt ccagcacctc
    12841 gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg atgccaggtt
    12901 tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata attttggttc
    12961 gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc gggtcgcctt
    13021 tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca tctgcaccgc
    13081 tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct tccggacgtt
    13141 cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct gagaaattat
    13201 ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag tggaatgtat
    13261 ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct tttgcatatt
    13321 tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg gtaaaatcaa
    13381 tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg atcttgcctg
    13441 attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat tttttcccta
    13501 aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa ctattttggc
    13561 cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag ggtgcatacc
    13621 cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca tttgccagag
    13681 atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa accatatttt
    13741 ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga tgaaatgcgg
    13801 taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa ggccgctaaa
    13861 attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca ctgctaagac
    13921 agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag tgcacatttc
    13981 ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt taatgattct
    14041 cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac aaaaaaagtt
    14101 tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa atactgataa
    14161 atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc tattcattgc
    14221 aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag aactagaaca
    14281 gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc tttggctcac
    14341 gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt agataaatca
    14401 aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat gtaatggcta
    14461 aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca ttttctttgc
    14521 gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata caagccagaa
    14581 taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt atacagcagt
    14641 attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt caaatgccca
    14701 taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc gagtatgggc
    14761 tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa gcagcaaaag
    14821 agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc acaccttctg
    14881 gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag ccggaatact
    14941 tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt gtcaaaaaga
    15001 tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat ctagcgaaag
    15061 aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag gctaaaagcc
    15121 cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa tgtcaacaaa
    15181 cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga ggacaaatac
    15241 gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag caggcggctt
    15301 atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa gttcatccgt
    15361 tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc aatcaaacgc
    15421 ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt gttttcaaaa
    15481 aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga aaaaatgatt
    15541 gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat gtttgcaaga
    15601 agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca attgttgaaa
    15661 gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa ttttgattac
    15721 ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac atttttagat
    15781 gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca gctttacacg
    15841 gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc aaaacccatt
    15901 aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg tgtcactcta
    15961 ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc cgacaatgta
    16021 gtgattgcag gaaatcctgc tcgtatcatc aaggcagtag aggaagaact aacagaagag
    16081 tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttggt tgctttatta
    16141 gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga aaaccaagca
    16201 gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct gctgaaaaat
    16261 aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca ctcgcgcgaa
    16321 taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg cgaaaacaaa
    16381 tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt gcgaaaactt
    16441 ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct gcaggtaaaa
    16501 gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat actgggcaca
    16561 tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg agactaatcg
    16621 ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac ggtgagttag
    16681 caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga cgttatcaaa
    16741 aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat acgatccgct
    16801 acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca ggaatcctct
    16861 acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat catctctgag
    16921 ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc caatacacag
    16981 aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg gagaaacata
    17041 aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct tgtagcatag
    17101 tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt tagtgcaaat
    17161 ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc gatttcatgt
    17221 tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc attcatatca
    17281 taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc tcgatcaaca
    17341 gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct caaaatttta
    17401 ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg acttggtacg
    17461 tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc aacaccgcct
    17521 cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa ctcaagcatc
    17581 tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt tgcaacttcg
    17641 cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga atatgtaccc
    17701 ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca caaaaatgga
    17761 aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta cttgattttc
    17821 aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc cacttggtta
    17881 agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt gaaagaaatg
    17941 gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca tgaaggggat
    18001 actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca cttgaatgaa
    18061 catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa aaaagttggt
    18121 caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta ccgcgaattg
    18181 atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt cacatgaatc
    18241 tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta gatggactcc
    18301 tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt tgtttagtag
    18361 cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag tattcaaaac
    18421 gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac aagcaaacgg
    18481 agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg cctctaaaga
    18541 agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa tgtatgcagc
    18601 agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg aagcaacatt
    18661 gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct tccgtaatga
    18721 agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag ctatggcagc
    18781 aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg gcccaatcgc
    18841 aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag tagaacaagc
    18901 agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca atatggttga
    18961 aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt ttggttttga
    19021 cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag ggaaagaaaa
    19081 aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct ttgatgcttc
    19141 ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg aagataatat
    19201 cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag gtcatgaaga
    19261 tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa aagatgtcgt
    19321 acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg atgaaatccg
    19381 tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt tgttcacacg
    19441 aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc accaaatcat
    19501 tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt tccctcaatt
    19561 ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg gacatggcgc
    19621 attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc cttatacgat
    19681 ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa gtatctgtgc
    19741 tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg ccggtattgc
    19801 gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc aagggctgga
    19861 agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta tcactgcttt
    19921 acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag cattaactca
    19981 agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg ctgctccacg
    20041 tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac ctgctaaaat
    20101 caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg aaactggtgt
    20161 gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg cagaaatgat
    20221 caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg gtgaagttta
    20281 tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga tcaaaggaaa
    20341 agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag tcgaagatgt
    20401 tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac aaggacgcgt
    20461 aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg aagaaaaata
    20521 atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga caataattat
    20581 tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg aataaacggt
    20641 gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt aggagctgtt
    20701 tttacttggc tgttcttatt acttgatgca gaacagcttt tttacaacct ctccaataaa
    20761 cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa cggcaaaata tgaggaacta
    20821 tttttccgtt tgtttctcta atactcaggt caaaccgctg atttcacaac ctctgattca
    20881 cggtgttcca gaagcagctt ctacggaaat aagctgaaca cttttgttac aacttctttt
    20941 tttctggagt ttcttggagc atatttttgg agtccttctg aataaagcgt aaaataaaag
    21001 tgagaaatat ggataaagga gggaaaggag atgctgaatt ttttatggtc gttatttatc
    21061 ggcggtatat taggtttgat agctggcgca attttaggaa aggaagtccc gggaggcgta
    21121 tctggaaata tcattatcgg ttttttaggt agttggctcg gcgaattttt actaggacca
    21181 ctgggaccgg aactcggcgg tttttatttg ataccagcat tattgggtgc aattttttgc
    21241 cttgctcttt attcatttgt ggcaagacgt atcagagaac gtccaaagaa agaataattt
    21301 tattcaaata agttgacact tcagtgtgtt acttggataa ttagaagcaa tttaggaaag
    21361 cgggtgtcat ggataagaat atcatctatc attcaagctt acctaaagga tatccatata
    21421 acaagaaatg agggaaaaac gtggcaacaa cgcacaatac caaaaagata tcaatggctg
    21481 gcctgcttgt agcgatggga gtcgtctacg gagacatcgg gacaagtccg ctttacgtta
    21541 tgaaatcgat tgttgaagat aatggcggat tgtcaggaat caatcctgat tttgttatcg
    21601 gatcagtctc attgattttt tggacattga ctctactgac aacaatcaaa tatgtcgtga
    21661 ttgcattgaa tgcagataac catggtgaag gtggaatttt ctcactctac acacttgtcc
    21721 gaaaaggtgg gaaatatttg atcattcctg caatgatcgg aggagctgcc ttactagcag
    21781 atggggtatt gacccctgct gtgacggtaa cgactgcgat tgaaggcttg cgaggaattc
    21841 cgcagttttt tgaccggttc ggtaataacc agaacgtgat cgtcattatc actttagtta
    21901 ttatttttgt tttattcatg gtccaacgct ttggtacaga tattgtcgga aaagcattcg
    21961 gaccaatcat gttcgcttgg tttacttttt taggcgtaat gggattaatg aacttcagtc
    22021 aagactggac agttatccgt gcactgaatc cttattatgc tatccagctg ctttttagtg
    22081 cagataacaa attaggcttg ttcattcttg gaaatatttt cctggctacg acaggtgcag
    22141 aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa tattcgtctg agctggcctt
    22201 acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc tgcgtggttg ttgaatgttt
    22261 atcaaaaccc tgaagcacag caaatcaaga acctgaatcc attcttccaa atgatgcctc
    22321 aaagttggat cgtgatcggc gttgcttttg caacagtcgc tgcagtcatt gcttcgcaag
    22381 cattgattac aggttcgttt acgctagttt cagaagcaat caagttgaaa ctgctgcctc
    22441 ggttaaagat catgtatcct gggaacagta tcgggcaaat gtatatccca gcagtcaatc
    22501 tgattttatg gatcgcctgt tctctagtgg ttgtcacatt ccgaacgtct catcatatgg
    22561 aagctgctta cggtctatcg atcactgtga cgatgttgat gacaacagca ctgctttact
    22621 tttatcttat ccaaaatggg cattctcgtt ggcttgcata tttagttacc tttttcttcg
    22681 gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa attcttccga ggcggttttg
    22741 tagccgttct gattggtttg atcatcttag tcgtgatgtt tatttgggaa caagggaaca
    22801 ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga ttatatcccg cagcttagag
    22861 aattacgtga tgatacttct ttaccaatct atcagacaaa tgtggtcttc ttggttcctg
    22921 atatggctga agataaagtt ggccgacagt tcatctattc gattttagat aaacggccta
    22981 aaagagcacg agtctattgg tttgttcatg tggaagtgac ggatgagcca tatacgaaag
    23041 aatatcaagt agatatgatg gacactgatt ttattgtcca agtgaatctg ttcttaggct
    23101 tcagggtcca acaggaaatc aacgtatatg ttcgccaagt catccatgat ttgatgaaac
    23161 aaggccgttt gccaaaacag ccacaaagat actctctgac accagggcga gaagttggcg
    23221 atttccaatt tgtcctgatt gatgaagtag tttccaatgt gacaactcta ggaaaatggc
    23281 aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat cgctacctcg ccagaaacat
    23341 ggtttgggct agagtacagc gaagtaaaac atgaaactgt tccgcttatc attggaaata
    23401 ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg ctttgacctg aactaatgtc
    23461 gaatgtcagc tacttgaaca ccgtttatca gaggtcatga aaaagctgta ctgcaacaaa
    23521 cagtaagaac gagcagataa aaacagcttc tcatgttttt catctgtttg ggcttaatgc
    23581 cgcagatgca agcttttgaa taccgttttt atcagaggtc atgaaaaagc tgtttagcaa
    23641 caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc cgcatctcct
    23701 tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa aagggtacaa
    23761 tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat ttttgatgat
    23821 tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca gacgatcaag
    23881 attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc atgttacaca
    23941 ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg gtttgatcat
    24001 cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg gtaaactacc
    24061 ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt tagttacgta
    24121 tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca atacggagaa
    24181 ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt ttccttttat
    24241 ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc gtctcgtatc
    24301 tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga attcttatga
    24361 aaaaggagag ttgaccaaag aagaatatca ttatttggaa aacgtgtttg aatttgatga
    24421 taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg aagcttcaga
    24481 aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct atccggttat
    24541 cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga tcaagcaatc
    24601 ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg taacaactga
    24661 aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggaaagc atattgctat
    24721 cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata ttttagaaga
    24781 aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag cggaacagtc
    24841 agatggctct tatattatct ccggcaaact aacattagat gatttccagc gttattttca
    24901 tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct ttgcttctag
    24961 ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc gtttcacggt
    25021 attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg aaagaaaaac
    25081 agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttccta cgattagtca
    25141 agtaaaaggt gcaagaaaaa tagctcgtaa acgaaagcga caaaatcaaa gcacaccaaa
    25201 taaagccccg ttcgtaaaaa attttcgatc tgggcaaaga agtggtatac tgtaagtggt
    25261 ttagttaatg gtccactgag accgcgtacg catagtcgta ctctgtatgt gccctaacca
    25321 tagcgtgcat acatttcaga agcttgttca tacaagcaac tgtggcaacc ttatcttt
//
LOCUS       DACEKG010000041        25289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEKG010000041 DACEKG010000000
VERSION     DACEKG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.041"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..552)
                     /locus_tag="IW664_001573"
     CDS             complement(<1..552)
                     /locus_tag="IW664_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0848938.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTT"
     gene            860..1666
                     /locus_tag="IW664_001574"
     CDS             860..1666
                     /locus_tag="IW664_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0848939.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1678..2898
                     /locus_tag="IW664_001575"
     CDS             1678..2898
                     /locus_tag="IW664_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0848940.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2888..4474
                     /locus_tag="IW664_001576"
     CDS             2888..4474
                     /locus_tag="IW664_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0848941.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            4513..6651
                     /locus_tag="IW664_001577"
     CDS             4513..6651
                     /locus_tag="IW664_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0848942.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6939..8330
                     /locus_tag="IW664_001578"
     CDS             6939..8330
                     /locus_tag="IW664_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0848943.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            8389..9198
                     /locus_tag="IW664_001579"
     CDS             8389..9198
                     /locus_tag="IW664_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0848944.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            9170..10159
                     /locus_tag="IW664_001580"
     CDS             9170..10159
                     /locus_tag="IW664_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0848945.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            10273..11268
                     /locus_tag="IW664_001581"
     CDS             10273..11268
                     /locus_tag="IW664_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0848946.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            11272..12414
                     /locus_tag="IW664_001582"
     CDS             11272..12414
                     /locus_tag="IW664_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ0848947.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            12411..13838
                     /locus_tag="IW664_001583"
     CDS             12411..13838
                     /locus_tag="IW664_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0848948.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13905..15194)
                     /locus_tag="IW664_001584"
                     /pseudo
     CDS             complement(13905..15194)
                     /locus_tag="IW664_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15374..15784)
                     /gene="tagD"
                     /locus_tag="IW664_001585"
     CDS             complement(15374..15784)
                     /gene="tagD"
                     /locus_tag="IW664_001585"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ0848949.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15910..17301
                     /locus_tag="IW664_001586"
     CDS             15910..17301
                     /locus_tag="IW664_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ0848950.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            17434..18381
                     /locus_tag="IW664_001587"
     CDS             17434..18381
                     /locus_tag="IW664_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0848951.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(18421..19581)
                     /locus_tag="IW664_001588"
     CDS             complement(18421..19581)
                     /locus_tag="IW664_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0848952.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19746..20921
                     /gene="mltG"
                     /locus_tag="IW664_001589"
     CDS             19746..20921
                     /gene="mltG"
                     /locus_tag="IW664_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0848953.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            21057..21536
                     /gene="greA"
                     /locus_tag="IW664_001590"
     CDS             21057..21536
                     /gene="greA"
                     /locus_tag="IW664_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0848954.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21704..22435
                     /locus_tag="IW664_001591"
     CDS             21704..22435
                     /locus_tag="IW664_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0848955.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            22432..23499
                     /locus_tag="IW664_001592"
     CDS             22432..23499
                     /locus_tag="IW664_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0848956.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            23506..24138
                     /locus_tag="IW664_001593"
     CDS             23506..24138
                     /locus_tag="IW664_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0848957.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            24278..24943
                     /locus_tag="IW664_001594"
     CDS             24278..24943
                     /locus_tag="IW664_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0848958.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24971..25264
                     /locus_tag="IW664_001595"
     CDS             24971..25264
                     /locus_tag="IW664_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0848959.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8595 a         3790 c         4898 g         8006 t
ORIGIN      
        1 ggttgtccca tcaacacaaa taaaactcat ggaaccagaa acagaattca ttgatttgaa
       61 ctcatccata cacaacaccg tcggaaggaa gttcatacgt ggctggtaat gcttcgttgc
      121 ttcacgcaag atccgtgtca ccgtcccatc cgagacgaag tacttcttag cgattagttt
      181 acgtgattga ttttcgtaca gttccatgag aatcgcctgc ttcagttcct ttgttagttg
      241 atgatgttca tcaactaagt ccgttttcgc agaaaaggtc cttccacaat catggcagag
      301 aaaacgggta cgttttaatt ctaaaatagt taacttttct ttgactttca acatctgagt
      361 gcgtgtcgta taggaaccgt tgcggataat acgaaccgat cgacagtgtt cacaatagga
      421 aggagagtca tccaattttc ctttaataaa ctgcgcttga acgcctttat attctttttc
      481 aatcagccat tcttcatcaa aaaataaatg tttatctgtt aatccgagta attttctagt
      541 atgattatcc attgagagac tcctttggta ggatgacgtt ttggtcgact aaattttacc
      601 aaaagtgagt ctctttttga gtttaaacga agaaaaagtg ttagtagaaa atcgccttcg
      661 attttccacc aacacaaaat attatagaac cccttttttg gtttattgca attgtctagc
      721 atcttctgta aaatagaaac gttagagaaa atcttgtatc atagaaaatt aagaaagaaa
      781 taagaacgca gctcggtacc aaaaatatgg taaaatggca cttgactgta attcactgaa
      841 atcgcttgga ggaacacaaa tgtttaaaga tttgtattta tttatgaaag acatttatca
      901 gaatagaaaa ttactgctac agttttcgat caatgacttc aaatcaagat atgcaggatc
      961 gtttttagga atcttatggg cgtttatcaa tccgctattt actgtattga tttattggct
     1021 ggtatttggt gttggactga aagcacaaat gatcgatgca aaatacccat ttatcgtata
     1081 tttagttacc gggatgattc cctggttctt cttctcagat gcatttgcat caactacggt
     1141 tgtttttcgg gaatatactt atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc
     1201 cacgacgaaa atcctatcca atctgtacac ccatttattc ttcattctga ttggcatagc
     1261 tgtcgcgctt ataaatggta tatatccttc agcaatgtct ttacagctga tttattattt
     1321 attttgcatg tgtgctttct tgacaggatt gacttggctg acggcatcga tccaaccatt
     1381 tttacctgat atcatgcaat taattacgat cgtgctgcag ttgatcatgt ggacattacc
     1441 gatattatgg agccctaatc agtttgattc aggtcttgta agcatattga aattaaatcc
     1501 gctgtactat gttgttcaag gttatcgtga gtcattttta agtgaagctt ggttctggga
     1561 gcatggaaca tatactttgt atttctgggt tttcacgtta gtaatgttcg taatcggctc
     1621 tgcagtgttc cgtagattga aaccacattt ttcagatgtg ctgtaaggag aatagctatg
     1681 acagaatatg caattagact aaaagatatc acaaaatctt ataatatgta cgcaaaacct
     1741 tcggatcgat ttcgtgaagc cctcaatcct tttaagaaat cctatcatga tgtattttat
     1801 gcgttgaaaa acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa
     1861 aacggatcgg gaaaatctac aatgttgaaa atcattacgg gagtactgac accaacatcc
     1921 ggcacgatgg aaatcgaagg aaaaatctca gctttgctag aactaggatc aggattcaat
     1981 cctgagtatt ctggctatga aaatatctat ttaaatggaa tggttctagg attttcaaga
     2041 gaagaagtcg atgaaatggt cgatgatatc attgaatttg ctgatattgg cgaccatatt
     2101 tatcaacctg taaaaaccta ttcaagtggg atgttcgttc gtctagcttt tgctgtagcg
     2161 atcaatgtga atcctgatat tttgattgtg gatgaagcat tggcagtagg agatcttgaa
     2221 ttccaactga aatgtatgga aaaatttaca gaaattaaga actcgggaaa aacgatttta
     2281 ttcgtttctc atgatattag ttcgattcgc cgtttttgtg atcgttccta ttggttgaaa
     2341 aatggagaag ttgtcgaata tggtgatacg atggaagtaa cgacgaatta cgaaaacttt
     2401 ttgaagaaaa aatcagttaa aacagtagat cgaaataaaa accaagtaga acgcttcgca
     2461 gctccagata tcgtagacgt gacgaaagcc gaattgttgg ataaaaataa aaatccagtg
     2521 gatatgctgg aacaaggaga aaaactatac gttaaagtaa cctatgaagt aaaagacgat
     2581 acaatcaaaa atccagtttt aggaattgct ctgcgtacag tcgacaattt ttatgtgtgc
     2641 ggactcaata ctctactaga tggtatcaag atcccttgga aaaaaggtga gaatatcttt
     2701 tatctggagt atgaaaagtt aggtttgtta gctggtgaat attattttga cgttgcggtc
     2761 tttgaagaaa atgcaacggt tcctttggta tacaagacaa aatacatgaa cttgtttgtc
     2821 agcggttctt atatcggaga aggcatcgtg gtcttagacc ataaatggga agaagggact
     2881 catagcaatg aaatatgatt ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat
     2941 tgccgcacag atcaaaccag gaagtaaagt tttagagttt ggtcccggaa acggtcgatt
     3001 aacgaaacat ttgatcggag caaaacaatg tgaagtgagc attgttgagc tagataagga
     3061 attattcgat tttgtcagcg aatttgccca agatggattt tatggagata tcgaaagttt
     3121 tgaatgggca aactattatg ctggacagac atttgattac gtcttgtttg cggatgtttt
     3181 agaacattta gtagatcctg gaaaaacatt gaagaaagtc agagaatttt tgaatgaaga
     3241 aggagagatc ctgattacat tccctaactt agcacataat tctgtcatga tcgatttgtt
     3301 caataatcaa ttgccttggg cttcttatgg tctcctagat gaaacccata attcttttta
     3361 tactcatgat ggtttccaaa aagtttttga aaaagccgga ttattcatta atattgaaga
     3421 ttacttgtac ttggctgtag gagatacaga attaaaatca acttatgaag agttgccgga
     3481 agctgtacgc tatgatttca aaatgcgacc atttggtgaa gtctaccaat actttttctc
     3541 attgatgaaa catccagtag tacaaacttc cattgcacag ccacaaaact ctaattatgt
     3601 aaaagtacta gaagtaaccc agcaaacgaa acaagatgaa acaatccaac aaataccatt
     3661 caataatttc acaggggaaa atgagacact aagtttcccc gtgtcagaag caacagaaag
     3721 aatggtgttt cgcttcgcac agcaacctag ctttatcgaa ttcagtgcag aagctgctgg
     3781 agaaaaactc atatttatcg attcaaatgc tgtagtcaaa acagtaaatg attgttattt
     3841 gtttgatggg aaagaaatac cagagttcgc tctaacggat atttctggga aagaagtgac
     3901 tatccattgt cactatcgct ttatcgggga attgacacca acaatgaaag aattgctgga
     3961 aacaattcgt cctatggctg aagtcacaaa gcagctttct gagaaaaatg atgaattaga
     4021 aagagaaaat cagcatcttc ttgaggaaaa tacaagacta gaccatacgt taaaaacaac
     4081 cacaaatcgc tattgcacat tgttggaaag tgatgaatgg acgatcaaac atagactagg
     4141 acgacgtaag aaaaaaacat cgaaaaaaat acaagaaaaa gagctttcta tatgtattga
     4201 tgagaaaata tgggatgcag aaacaaaaat attaaaaatc atcggatggg gaatcgctaa
     4261 ttcggatcgc cagccattgt cctataagtt atcagctgac caatcgccat ttttcgaagc
     4321 aattcctgtt tcacgagatg aagtcaatca agcagaacag ctcgcaaaag gaacagaagc
     4381 gggatttgaa ttacgaatcc tttgcgagca agaacgttca ttcttagtgg aagcagtagc
     4441 tcaaaatggt caatcatgga tcattgaaat gtaaataatg attaacttat aaagataagg
     4501 agtaaacgaa tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa
     4561 cgggccactg gcgatttgct tattgtgggc tgggcaattg atgaagtgac caaagaaatc
     4621 cctacgataa aggtagaaaa agaaaatgtg attgctgaag caacacatgt cgtaagattg
     4681 gatatcaatc atttatataa tcttgatgtt aagacccaaa gcggttttaa gatacgttta
     4741 tccgggaaaa tgagaggaaa agcgatctta gattttcaaa cggcaaaaca tcaaaatggc
     4801 atagctgtga aactaaatgg taaataccct tatgatgacg gcattgaatc ttcatgggaa
     4861 agaaaaaaaa gattactgaa aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa
     4921 acaatccgcc gggtcaaact ggaattgaaa ccaggatcga tcgactatgc agagtggatc
     4981 agtcgtcacg agaaacctga tctgaaagag caaaggaaag aagcacaagg gtttgcatac
     5041 cgtccattga tttccatact tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa
     5101 tgcattgatt cggtattgga tcaaacgtat gatcactggg aactatgtat cagtgatgat
     5161 gcttctactg atccagctat ccgaaagtgt ctggaatcct atcaagcaaa ggatgataga
     5221 atcaaagttg ttttccgaca agaaaatgga catatcagtc tggcaactaa ttctgcgttg
     5281 gaaatggcag aaggagaatt tatagcgctc ttagataatg atgatgaatt accgccattt
     5341 gcattatatg aagtggcaaa agtgctaaac atacatccag aactggattt gatctacagt
     5401 gacgaagata aaatcgatgc agatggcaat cgctttgatc cgcacttcaa agcagactgg
     5461 tctcccgata cattgatggg aaataattat atctctcact taggcgtata tcgaacaagt
     5521 attgtgaaaa agcttggcgg tttccgtaaa ggctacgaag gctctcaaga ttatgatttg
     5581 gtccttcgtg tgacagaaca aatccctgca gatcatattt accatattga ccgagtcttg
     5641 tatcattggc ggacgatccc aggatcaacc gcaagtaatg gtgaagccaa aagttatatc
     5701 tatgattctg gagtgaaagc attgacagat gccctgtcta gaaggaatat caaaggaagc
     5761 gtacgtcctg gcagaatttc tggattttat gagattgctt atgacgtctt gcaagaggat
     5821 ttggtcagcg tgatcattcc aacgaagaat ggctatgaag atctaaaaac ttgtgtggat
     5881 tcgattatcg aaaaaacgtc ttatccaaat tatgaaatca ttattgcaga taatggaagt
     5941 acagatccta aaatgcagga attatttgca gaatacaagc atcagctgaa agaccgattt
     6001 atcgttgaat taattgacat tccattcaat tattcacgta tcaacaatct ggcagcagaa
     6061 aaagcaaacg gcaaatactt tttattcttg aataacgata cagaagtgat tgaacctgac
     6121 tggatgacag cgatggtttc ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa
     6181 ctgttttatc cagatgatac aacgcagcat gcaggagtat tattaggaat cggtggtgtt
     6241 gcaggacatg cattgaataa ctatgatcga acacattgtg gttatttcgg ccgattggtc
     6301 attgacgtca attacttagc agtcactgca gcttgtatga tggtcaaggc agctgacttc
     6361 aatgctgtca atggatttga tgaaacatta gaagtagcat tcaatgatgt tgacttgtgt
     6421 ttgaaagttt atgaactggg tcgttacaat gtctatgcac atcaagcaga attgtatcat
     6481 ttcgaatcta aatcaagagg ttatgaagat acaccagaaa aacaaagacg ttttgccggg
     6541 gaaataaaaa aaatgcaaga taagtggcct tcttatattg ctcatgaccc attttataac
     6601 gataatctaa ctaaagaagg aatcggcgat ttttcattga gacctgattg attaaaagaa
     6661 ctgtattttc atttttattg atgaaaatac agttttttta gtatatgaaa attgtaactt
     6721 gtgaccgaag agatgatagg aagaacaccc attcagtagg aagaatgtga tacgattttt
     6781 gtccaactct ctgttaggca gaaatataca tataaacata caatagtcac cttttctagt
     6841 ttcttttaat ttattttaca atttggttat tcactaggtt tttaaacttt gctatggtaa
     6901 gatgatagag gcattatttt atggttagga gtgtatatat gaataaaaat ggggagtgga
     6961 atgcggcacg taggatattc attatcatca tggatgtact tgtattgaat gtgtcgatct
     7021 atcttagctt tttgctgaag ttccaaggag aagtcccagc aagaaactta gagacctttc
     7081 agcattcagc agtttttatt tccattatct ttattgcgtt gaatattttg ttaggagctt
     7141 atgtattcta tagccgagtg atcagtgata tcgtcttcat tactgttatt ggacaggtgc
     7201 tgatagcatt ggggatcatg gtagttactt ttgctggacg ttggtttgca tttccgcgtg
     7261 gcgtcatcgt gctctcgttt atacttggca cgatattact tagcgtttat cggatagttg
     7321 tttataaact atacttaaga gtcagtcgtg ataagaaagt ccttatcgta ggtttggaga
     7381 aagatgtcgc accagctatc caaaactttc aatctaagaa aaacaacaaa cataaagttg
     7441 aagcagtagt gatcgacaag tattacgaaa acacaaaaaa attgattgat gatatcgata
     7501 ttgtctactt ggcttctaaa atcccagaag acgaaaaaat caatatttat caattagtaa
     7561 ctaaaaaaga gaagaagcta tttttaaata caacttttga aaatttaacg atgatcaatc
     7621 cgaacatcat gaatttcgaa gatgaaagta tcattgaagc ttccggtttc aaaattcagc
     7681 cggaatatga attattcaaa cgtctttttg atatcatcgt ttcgcttatt ttactgattc
     7741 ttgcttcacc ttttatgcta atcacagcga ttttggtaaa aacaacttca cctggaccaa
     7801 ttatttataa acaagtacgt attacgaaaa atcaaaagga attttcaatc tataaatttc
     7861 gttccatgag tgtaacagct gaagcaaaat caggaccggt cttggcaaag agcaatgatg
     7921 cacgtgtgac accagtaggc aaatttatcc gtgccgtacg ttttgacgaa ttacctcaaa
     7981 tctttaatgt attgaaaggc gatatgtcga tcgtcggacc acgtccagaa agaccatttt
     8041 tcgttgatca gttcaatgaa gaaaatcctt attattattt aagacacaat gtacgtgcag
     8101 ggattaccgg atatgcacaa gtatacggaa aatatgtatc agattataat agtaaactac
     8161 gttttgattt gttgtatatc aaaaagtatt ccttactatt agatttgaag attttacttc
     8221 aaacaatcaa aatcttattt gataagatgt cctcacaagg actagatgaa gatgaaaata
     8281 tccgttcgtt agcagaaatc gacttttcac aagagaaaat ttatcgctag atgggaaaaa
     8341 ttttgagcaa agaagtagga gagaacattg gaaaataaaa atataagtat gctgctttct
     8401 ttatatgata aagaaaaacc agagtattta aaggaagcat taactagtat ttttgagcaa
     8461 acagtaatgc caaaagaaat tgtattagtt tatgacggcc ctataaatga aaaacttcaa
     8521 gtagttgtca cttatttcca acaaaaagat cctcacttgt ttacagttgt aaaattagaa
     8581 aaaaatcaag gattggggat tgctttagca attggattag aacatacgaa aaatcgctta
     8641 gtagccagaa tggatacaga tgatatcatg gaaccccaac gattagaaaa acaattagag
     8701 atcttccaaa aagatccaga aatagcgatt gttgggagta atattgagga atttactggc
     8761 acgcttagca atgttatcgg aagaaggatt gtacctgaat ataatgatga aatccgtgtg
     8821 ttttcgaaaa gaagaaatcc ctttaatcat atgacagtca tgtttgataa aaatgctatt
     8881 ttagaagtgg gaaactatcg cccattaatt ggatttgaag attattattt gtgggtaaga
     8941 ctgttgaagg cgggatataa agggtataat atccaagaga ttctagtcca tgctagagca
     9001 ggtgctgaca tgtatgctag acgtggagga agtaaatatc ttttaccagg aattaaaggc
     9061 cgatatcgcg tatggaaaga tggtctagga tcactaaaag attttctgat agttatttgc
     9121 ggacatcttg taattagttt attaccgaat aaaattaggg gaaaagtata tgaatcaaaa
     9181 cttagaaaaa caaactaaac tttctataat catacctttt tacaatgcaa aaaatacttt
     9241 aaaaagagct gtagacagcg tcttaacgca aggttatccc aatattgaaa tcttgttaat
     9301 tgatgatgga tctacagatg gtagtagtga cattggtgac gaaattgcta atctagagag
     9361 taaagtagca tgttatcact tggaaaacgc tggtccatca gttgctagga attgtgggat
     9421 aaaagaagca actggaaatt tggtaggatt tttagatagt gacgattatt ttttacctga
     9481 ttgttttaat aatgttttga aagactacca taggaattac cccgatatca ttgcttttgg
     9541 attaagtaaa ggaaaaaata tagaaaatgc aaatttattt gttcccgaaa agaagattgg
     9601 ttgttcaagt gaagaagcaa ttgagcaaat gtttagaagt aaggcagtgg atttttacgt
     9661 atggaataaa ttttttaaaa gggaattatt caatcatata gtttttccag aaaataaatt
     9721 atatgaggat atggttccta tgtatgaagc ttttagagcg gctagtaaaa ttgatatttt
     9781 gccattttca ggtatttttt attatcaaaa taatgatagc atcgtttatc agtcttttaa
     9841 ttcaaaacag tatgataata ttttgcaaag aaaaatatta gttgatgaag taaagaaaga
     9901 ttatcctcga cttattaatt tggccaatgc tcgattagta gacggatacc tttcaacagg
     9961 gtttaaaatt actggcaaga atcaaaatca gaaagaaaag caaaaatatt ataatcagtc
    10021 taaaagagaa ataaaaagta tatattcaaa aataagaagt aacaatgaga tatcaattgc
    10081 taaaaaatta gctttatttt tatacttaat taatcctaaa acttatttta ttttatataa
    10141 aaaagttcta aaaaaataaa gtttttttcg atatgttatt ttttatgttt attaacttcg
    10201 gaagactaaa attgttactg gtaatgatac atttgtaaaa ataaaaaaga tgatttttag
    10261 gaagtgaaga gagtgcataa aattagtgtt atagtacccg catataacgt agaagactat
    10321 ttagaaaact gcttagaatc tatttccaac cagacacatt caaatattga aataatagta
    10381 gtcaatgatg gttccccgga tcgaagtggg gaaattgcag aagaatataa aaagaaagat
    10441 atgcgattaa aagtgataca tcaagtgaat ggtggtttaa gtgcggctag gaattctggt
    10501 cttgattctg caacaggaga ttttgtgtgc tttattgaca gcgatgattg gatagaacca
    10561 gactatcttg agttactata ttttggcacg caaacttatg gagcagatat ctcggtgcta
    10621 aaaataaaaa agatttcaag tttagaacaa ctaaaagaac atacaattgc tgagagaaaa
    10681 tgggaaatat ttgatagaga acaagcaatg aaaaacttat ttataaataa taaaattggc
    10741 tattctgcaa ataataaatt attcaataga aatttatttg ataatataag attcccactg
    10801 ggtaaattaa tggaagataa ggctactact tatttgctga tcgatcattc cgaaaaagtt
    10861 gctgtcaatt catctgctaa atatcactat taccaaagcc ctaaaagtat actgagagga
    10921 agcttcaatc ctagaatttt tgattctttt gaagtccatg aagatatact aatattcata
    10981 gataagtttt atccggaatt atcaattaat gttagaactc gctatgtcta tgaagcaata
    11041 aggatgatga tgacaatgat taaaaataac tataccaata aagaagatta tttacgttct
    11101 ataaatattt tacaacgctt tgaaaaagaa gtgaaaattg atcccgactt ttcaccaaaa
    11161 ttaaaaaaat tagtaaagtt tttattaata aataaaaact tgccttacta tatgtcaagt
    11221 agtaagatag caagtaaatt cttgattaag atagaatctg tgaggtaaga gatgaagaat
    11281 ataaaaaaat ttttttcaag gaatattttc ccggttttta ttaaaatatt acctattcaa
    11341 aaaaatttaa ttgtattttc aagttttggc gggcgtaact attctggaaa tccaagagtt
    11401 atttcagaga aaattcatga aaaaaaattg aattacagaa ttatttggtt aatcaaaaaa
    11461 gaagcgatta taactgattt accagattat attaaaagaa ttgactccga ttctttttta
    11521 gctgcctttt atttatatac agctaagtat tggatagacg atagtcggaa aacgattaag
    11581 tatgcaaaaa ggaaaaaaca actttatttt caaacatggc acggaacgcc tttaaaaaaa
    11641 attgaatttg atgcaaaaga taagttacct aaacgttacc taagctatgc gaaaaaagat
    11701 agtgagtcaa ttacatattt attatctggt aatcgctata gttctaagaa gtatgtttca
    11761 gcattcaata tcgttccttc taaaatatta gaagtaggaa ctcctagaaa cgatgaatta
    11821 gcctctagta aagagcaagt gtttgacttg aaaaagaaac aaatcaatgt cttatttgct
    11881 ccaacttttc gaaataatat tgaggacaac ggaattacgc aactagaatg gttaggtgtg
    11941 gataacttac gtgaattttt taaaaagcaa caacaagaat taaattttat gactaaattt
    12001 catcctaatg ttcatagtaa attagcaact gattctaaaa gtatagaata tatgaaaaaa
    12061 catcaaatta ctttaattaa tgatgggatt cctcttgaaa tgctttttga agagatagat
    12121 gttttaatca cagattattc atctattttt ttcgattttg cgctaacaaa aaaaccaatt
    12181 attcttttta actatgatga aaaagaatat agcttagaaa gaggttttta tatacagtta
    12241 agtgagttac caattataag cgtacaagat agttatggcc ttgttgatgt ttttaaaaac
    12301 caacaagaaa agcttttgaa aagttctcaa gagttacttg attatattgg aaattatgaa
    12361 acaggagcaa gtacagaaaa ggttcttgag ttgatagaga gggatataaa gtgaaaaaaa
    12421 cagtaaaaga tatattttat aatgcgatat atcaagtctt tttgattgtt ttacccttac
    12481 ttactatacc tatacttagt cgtagaattg gttcaactgg tttagggatt tatgggtatg
    12541 ttttctcaat atcacagttt ttaatgactg tcattgctgt gggaatgaat ccttttcgta
    12601 ttcgaaatat cgctaaatca aggaaagata aaaaagcact atctttacaa ttctggaata
    12661 tttactttat tcaattttta ataggcttga gtgtttcctt tctctatata gcctatattt
    12721 atttttttga tgtaaagtat aaaaatttat acttaattca attgatattt attgtagggc
    12781 taacattaga tatttcttgg ttttttcaag gaattgaaga attttcgaaa gtggttatta
    12841 gaaatacagt tatcaaatta tcatcggtat tgttaataat catttttgtt tcaactaaag
    12901 atgatttgtg gatttacgtg ttcattactt caattactaa ctttttagga agtttagttt
    12961 tctggttaag tatccacgga aaagtaggta aaccaatctt taactctaat gtttttaaac
    13021 agttgtggaa accagctttt attattttga ctcctcagtt gtttatgcaa gtgtacacaa
    13081 ctttagacaa aacgatagtt gggtattttg tggatccaac ggaattatct tattatgatc
    13141 agtctcaaaa aattgctcgt attatattgg cagtcttaac atcaattact attgttatgt
    13201 tgcctaaaat atctaaagca caggcagagg ggaaatcaga aaatatttta gtttacacaa
    13261 aaaaatcttt tgattacacg attatcattg cattaattct ttttagtgtg gtgtttatca
    13321 atacaaaaga atttgttcca tggttttttg gggagaattt caaaccgatg acaataaaca
    13381 tgcttatttc ttgtctaatt atagttgtta gtccaattgg tggagttttt gctaaccaat
    13441 ttgcaattgc tatagaaaaa gataaggagt atgcttatcc aatgatcatt ggtgcgattg
    13501 taagtattat tggaaatgta gcttttgtgc ctacttatgg cgctgtagcg gctacgatcg
    13561 tactcgtggt agtagaatta ttagtctttt tctttagagt attttttgta cgaaaagaaa
    13621 taaaaatatc ctatttcttt gggaagaacg ttatcttatt ttttatagtc aatcttactt
    13681 tgactacttg ggcctattac tttcttccac aattattctc ttcttcattt ttagatatgg
    13741 caattaaatc aataatgatt ttcattattt atcttccgtt tgtttatttt ctttttccag
    13801 atatacgaaa tgaaataaga aagattactc gaaaataaaa taatgttgta aaaaagtagt
    13861 tctttgtcaa atcgggctga taaggtcatt tatgaacaac aaagttagta agagttacta
    13921 gcttttaatg catagctgat taacctgttg attagaaaaa tccacaagcg catattggta
    13981 aaactgcgaa acccataagc aaatcgtttc aatgttttga tatgtgtgcg aagattctct
    14041 aattttgtta ttagaatact ttttcgaaaa tgctagttaa aagccttttt cagatatgaa
    14101 aaaggctttt tgagatagtg aagtttcgag ggaacttctt tggtcgtttc tcgaagtagc
    14161 tagctgtttc ttgtgataat tgctaagcag cttttgataa actgcttagc aattttaagt
    14221 tcataagaga ttgagggtag acgatcaact acttcacttt ctgttaaata gcgccattga
    14281 aaattctgcc atttcttgaa tgttgtgtaa tccaactcgt catctgattt tagaagtaat
    14341 ttatacaaag gtttcaattg tcgatagtac ttagcttgtg aagtgtcttt ttgattcaac
    14401 tgcttcatca cacgttttcg aacacgatcg aaaaacttgt tcaagtgttg tccaatgtga
    14461 aagcaatcaa tgatgatttg ggcgtttgga aaggactctt tgactaatga aaaataaggg
    14521 gcattcatat ttgtgacaat gtatcgaacc cccatatgag cttttcttgt aaaacgcatg
    14581 aaataagcaa tcaaatcttt ttttctccgg ctggacaaaa tgtcaaagag cttccctgtc
    14641 tctctattca tacaagaaaa ggagaacttt ccatgggaaa ttgaaagaga gcgaaactcg
    14701 tcgaatagta aacaatgagg taaatagtct agcatctgtc gcagcattgc cgatagagca
    14761 gataatgtcc gttgaaatgt cgttactgaa agatcacaac gtttcgtaag tgtctttaac
    14821 gcttgacgct cggtcaactc ttctaatatc gtgtaatgaa cttgtttcga aataaaacat
    14881 gaaggaacga caagatacgt atgagcagta aaatattttc cgcatgattg gtagtggtaa
    14941 tgttgttttc taagacgcaa ataggtttat ttattcccac tgcgatttaa gataatcatt
    15001 gaaatcttct tgccgttttt cacgagctgc ttcgcagttt ctttacaaaa tagacaatgt
    15061 ctaaaagtgc gaactaatgt accttcgatt acgatggctt tcttgtcctt ataaaaagtt
    15121 ttttgaaaag aattaatttc aatattttta tctttaatag tgaagtgctc tttgatagaa
    15181 cgagaaatgg atatacaaaa tcttcctttc agattgatgt tttagacgac tctattttat
    15241 cgaaattttt gtatgtctgt tttttttaca agaaaatcta ggatcgacag aaaattctat
    15301 cagtcctaga aattatagag ccattttttt acgtcttatc cagatatatt gttctttcat
    15361 tgtttaaaaa atattattta tcactattta aagaattaag ttcgttcttt atttgagttg
    15421 tagagatctc aggtgttctt ggtaaataaa ttacttgtgc atctgttagt tgttcaagtt
    15481 catcaaattg tccagcccaa tcatcgccca tcacgaaata atctgcatgg tattctttta
    15541 tatcttttac tttttgttcc caattttctt caggaataac taaatcaacg tagcgaatag
    15601 cttcaagtaa ctgttttctt ttttcataac taaaataaca ttttttttgt ttcgcattcc
    15661 aattgaattc atctgtagaa agggcaacaa taagataatc tccatattgt tttgctcgtt
    15721 tcagtaaatt gatatgcccg tagtgaagca aatcaaaggt accgtaagta attactcttt
    15781 tcatctttca cctctcgaaa ttcattaata tacattgtta gtttagcatt tttttgttag
    15841 aattgaaaga accagtttat cctaaaaagg ataagaaatg tttatgagaa ataggtggat
    15901 ggaggaaaaa tgttgaatga aaaattttta aaatatgtga aaggttggta ttttatttat
    15961 tttggtttta ttctaatgtt acgagcaata ggcccgtatt tactattacc tactagatat
    16021 gatactttac ttttttggcc tgctggaata gcgggagttg tcttgatcat tatagatgca
    16081 attattgcat ttagagaaaa aaatcttaaa aaatatgatt ggctattaat tgcttttatt
    16141 ggcgcgatgc tagtaagcag tttaatgaat ttaaaatatg gatatagcga aaatatcaag
    16201 ttaatcatgt ggcaattaat ctttttcttt atgatttatc agtttggcaa agactacagt
    16261 agtaactttt ttttcaaagt atttacttgg atactttcat tggtatggtt tttagctaat
    16321 ttagtatcat tatactggtt tgtcattcaa tatggcataa aaattccaat aaaatataag
    16381 tattacccag ttcgtttagg gtggttaggc aaccgtttat ttggtgtata tacagatcca
    16441 aattttggtg cggttatatc attaattgtc atcattattg ctatatatta tttggtcact
    16501 tacaagcatt taaatcttct atttaaaata ttttttgcaa ttaatatttt tattcaattc
    16561 tcgtttattg ctttatcggg ctcacgcacc acattagttt gtttattaat agcaactttt
    16621 gtttttagtt tctttacaag ttatagtagt cgttttctta aaactaatac tttaaaaaga
    16681 tggctaactg cattagtagt aggattgcta agtataattg cgatgtatta tgtaacagat
    16741 ataactgctc aagcatgggc acatgttccg catattacag aaataaaaaa agatatatct
    16801 gattcatcag gaacaactag cagcaataaa gttgatttaa aacgccctga cactgatggc
    16861 ggggatgttt ctaatttaag gtttggactt tggaaaagtg caattgaaat attcaagagt
    16921 aagccgatat ccggcggttc tccaggatac tatattgaat acgcacatga taaactacca
    16981 tatacattaa tgggaaaaga tactttaact gctcataatt ttattttctt agttatggct
    17041 gctacaggag gattaggtct attgacattg ttaaccttct tatttatcca atgctttaaa
    17101 actttgcgtt attgtttcac acaaccaaat ctagtaaaag ataagcactt ttttgctatt
    17161 ttaatgattc ttacaatagc agtttcggca ttatttatca cagaactcat tttggttagt
    17221 acgataggtt catttatttt ttggggatat ctgggttcta tccaaaaaac tatatctaaa
    17281 gaagaaggag taactaattg aaactatttt taggtatata aagtagaaaa gaaaactagt
    17341 tatcccaact tcaacaaatt ttatagtttt tacaatataa aaatggtaaa ctaactttat
    17401 taagtatgag aatgcataat tggagtgaat ttgatggata ctatattgat tactggtgga
    17461 gctggtttta tcggttcaac gctagcgaat tatttgggaa aagaaaacaa aatcgtagta
    17521 gtcgacgatt tatcaatggg aaaaaaagaa aacttggata tggagaaaca agtgactttc
    17581 attgaggggg atgtatcaga tcctcaattg atggaaagaa tcatgaaaca gtatcagttt
    17641 gcctatattt ttcatttagc tgctgtagct agtgtggcag attctgtaga acgtcctcta
    17701 gaaactcatc gtgtaaattt tgacagtgcg cttttattgc ttgaattggt acgaaaatat
    17761 caatctggtt taaaacgatt ggtatttagt tcatcagctg cagtttatgg cgatgaacca
    17821 acgttgccaa aaaaagaaga atcggtcatc cgtcctttaa caccatatgc catcgataag
    17881 tttgcatcag aacaatatgt tttggattac tgtcatttat atgatgttcc gactagtgct
    17941 gtccgatttt ttaacgttta tggtccaaat caaaatccta actctccata tagcggagtt
    18001 atttcgattc tagtggaccg atacaaaaaa caattggcag gagaaaaaac agaatttact
    18061 ttatttggtg atggtagcca atctagagac ttcgtttata tagaagatgt tatacaagca
    18121 cttttacttg ttgctaagga agaaaaagct ttaggacagc aattcaatgt cggtacagga
    18181 aaaagcacta cgctacttga attgattcat tcaattgatc aaatcttagg aacagagcta
    18241 gcgcttaaat atgaagcgga gcgttcaggg gatattcgtg attctttagc ggatatttca
    18301 aagatccgtt ctttaggtta tcaacccaaa tttgatatat tgaacggaat ggaacgttat
    18361 ttaaagacag aaatcgattg aaacaaaaaa ccagcatcct gctggttttt ttgtctcgac
    18421 ttagcttttt aaattaacac cttccatcag ttcgtaaatc tgatttctta aatcagtggc
    18481 cggcaatttg atgtttttat cagattggat attactttcc atgacgattg catcttttgt
    18541 gtcttttttc atcgccgcta ctgcatggaa tcgattgata tttccctgag caaggatata
    18601 atcatttcca ctgtaaacac cgccgctgta aggatgttta tcaccattat tttgccacaa
    18661 tgagaaaagt agttcttttg gtataatatc gccagacaga acactggtaa agaatttgtt
    18721 caagtcataa acagacatac ttatatttcc tgtgcctaat tcatttcgga tgtctgtttc
    18781 aggctcgacc aatgcttttg aataatcgtc agttgcagac atttggtaag atacagcatg
    18841 ctgaggggaa ttttttacat tttcataaaa atctgtatgt tctagatgga gtggccgaat
    18901 gatttctttt tttaaaagag tttcataagg ctgattcatt agtttgatca atacccctgt
    18961 aagcaatgta taatttaatg gttcataatg atacgtatca taaccagtaa atttcaaatg
    19021 atgaagggca aactgaatga tctcactatc tgacataggt ttttttggag aagcggttcg
    19081 atgaagacca gagcgcatgt aaagcatatt tttgattgag atttgctgac ttccaggaat
    19141 ctgaggataa aattcagata gctttgtgtc aaaagttatc ttgccagatt gaatttgttt
    19201 taggataagt aaagcagtca tcgctttttg gatcgaaccg atttgatagt aaagagaagg
    19261 agaattcgat tcttttatgc gtagatttgc gtacccatat cctttttcta atatcactcg
    19321 attatttttg cgtacataaa tcgaaccgat ataatctgat ttttgcagta attgatcaat
    19381 tttgctggca agcatctgct cattagatgt taaattggaa gcaagttgtg cgggttccgt
    19441 tttataaatt tgtggttgag gttgttttgt taattgccat cctgtaatga tgcctgttga
    19501 aaagaagctg acagccagca acaaaactgc gagaaaagaa agagagatct gctgtttttt
    19561 ttgatgtttt ccttttgcca tattaaatcc tttctatcca tttttaacta tttttcattg
    19621 taatcctcaa aaagagcatt tgccacttac aattttgaca taatatttgg taaaataatt
    19681 cacggcagta gaaatttcat gtataataaa cagagttacg aagatgatag taaggaggag
    19741 ttgttttgtc aggaaacaaa caacacccaa gcaaagacaa aaaagaagcc agtaaggcag
    19801 ctacagcaga tataatcgac aagcggagaa atctgcgtaa aaaagaagat aagatcgtga
    19861 ggaagatcat tttagtgatc gctcttacat tattgattat tggcggattt ctcgggttta
    19921 ccgtatatcg ttatgtagat agcgggttga agcctttaga taaatcggac gaccaattag
    19981 tccaagtgga aatcccaaac ggctcttcta ataagcagat tggcgaaatt ttggagaaag
    20041 acaatattat taaaagtggg attgttttca attattatac aaaatttaaa aacttaactg
    20101 gctttcaagc tggttattat cagttagcgc ctaatatgac tcttgatgag attggcaagc
    20161 agttgcaaga aggcggcaca tctgagccaa caaaagttgc agatgggaaa attgccattc
    20221 cagaaggcta cgacatcgat cagattgctg agcgggtagc gaaggtaaca ggtaaagaca
    20281 agaaagaatt tttagattta gtaaatgatg agactttctt taacgagttg tatcaaaaat
    20341 tcccagaatt gttggaaagt gcctctaaag ctgagaacac aaaataccgt ttggaaggct
    20401 acttattccc agctacttat gattactaca cgagtacttc gcttaaggat cttgtgattg
    20461 agatggtgaa caagacaaat acggtcatgc aaaactatta ttcagctatc aaacagaaaa
    20521 atttgactgt acaagaagtt ttaactttgg cttctctagt tgaaaaagaa ggcgtaaaag
    20581 aaaatgatcg taaaaatatt gcacaagtct tctttaaccg aatcaaggca aatatgccac
    20641 ttcagtcaga tatctctgtt ttatatgcat taggagaaca taaagaattg gtgacgtatg
    20701 aagatacagc tgtagattca ccatacaatt tgtataccaa taccggctat gggcctggtc
    20761 cttttgacaa tccaagcgaa aaggcaatca aggcagtatt agaaccagca gaaaatgact
    20821 attattattt cgtagcagat acgagcactg gaaatgttta tttcgctaaa acgtatgaag
    20881 aacatatgga actagtgcaa aaatatgtaa ataattctta gaaaaacagg ggctagtcat
    20941 tgatcatttt tagaaaaaca tttacatttt aagaaaagcg tggtaatctt ttttggacta
    21001 tattcctcaa aagattacct tttgcggtat tttatcgaaa aaaggagacg ttacacatgg
    21061 tagagaaagt ttatccaatg actctagaag gtaaagaaaa attagaaaaa gaattagaag
    21121 aactaaaaac agttaaacgc ggagaaatca ttgaacgaat caaaatcgct cgcggtttcg
    21181 gagatctttc cgaaaactcc gaatacgaat cagctaaaga tgaacaagca tttgttgaag
    21241 gaagaatcac aacaattgaa aatatgatcc gttttgcaca aatcatcgat aatgacggcg
    21301 tagatgcaga tgaggtatcg atcggtaaaa cagttacgtt catcgagttg cctgatggag
    21361 atgaagaaga gtacacgatt gtcggaagtg ctgaagcaga cccgttttct ggaaagattt
    21421 ccaatgattc tcctatcgcg caagcattga ttggcaaacg taaaaatgat gaagtaacca
    21481 ttgctacacc aggtggcgat atgttagtta aaatcgtcaa agtagaacaa gcgtagtctg
    21541 actgaaagat ctagagagtt catcttactg atgacgcact ctagatcttt tctctttgag
    21601 tattgcaggt ttcactgaac gatcttacat ctaaagtcct atagccaaca gtcaaaaaaa
    21661 taggtatgat gaaatgagaa tgtgtcagga gggagaaaaa gcgatgaata gttcttggcg
    21721 tttttttgtg gtagtcgaag cactgctgct gttatttgct gtttggcaaa tcgtaaacaa
    21781 tactggactt ttgcttttag taatattcgg tattttcaat atatatctag ctatgcgcaa
    21841 atatcctcgt actaaatttc aaaattttca attgatctta gggagcttgg tcattttttt
    21901 tagcttagtc aacagtcctg ctttatggat gatggttgta ttggccgttt tatttatcgg
    21961 gctgaaaggt gtagagattt caggaataga tttgacaaaa aatgcttttt ggcgaaaaaa
    22021 acagatcatg atggtccaaa cagagcaatt aaaaacacat aataatgagc gtaaaagaca
    22081 gcaattattt ggtaaccaac gtattggaaa cgacgtctat gaatgggatg atatcaacat
    22141 tgccatcatt tctggtgata ctatcatcga tttgggtaat acacttttgc caaaagatga
    22201 taatattgtg attgtaagaa aagggatagg cagaacacgg atcttagtac ccctgggagt
    22261 ggctattcgt ttagagcatg cgacattagt aggaaatgtc ttgtttgaag aagagcagtt
    22321 ttccttaaaa aacgaacaga tcaaaatctg cagcagtgat tatgatgaaa atcctcgcag
    22381 attgaagatc attaccaata ccttggttgg agatgtcgag gtgatacgaa tatgatggga
    22441 agaatatcca aagcaatgct agctgtttat tcggggattg cggctttcct tattattcta
    22501 ttttcacttt tcacttattt ttatgccagt aaccaaagtc attggtgggg agaattgctg
    22561 cgtgcgcgtt tattatacgt tccgcttatc ttccatttga tggcaatttc attaggtgta
    22621 ggattgatcg tatttctatt gttatcactt attcaaaagg caaaatacgg gaaaatcgaa
    22681 gaaaagcttc gtgcactttc ttctggcaat tatgaatcca aacttttgca tcagccgatc
    22741 ccaagcgcgt cggatgatct gtacatcaaa gatattgata tggaaattgc taaaataaaa
    22801 gaaaaaatga tcgaagtatc cagtgaatta cagattatga ctagccgtcc tcaatatgta
    22861 gatggacaaa ccaaagaaga gattttagaa ttagaaaggc atcgattagc tcgtgagctt
    22921 catgattcag tttcacagca attatttgca gcgatgatga tgatgtcggc attgacggag
    22981 caagcagaaa aaagtgagac accagaaatg ttccgtaagc agttgaaaat ggtggcagag
    23041 atcatcaacg cttcccagtc tgagatgcgt gcgctgcttc ttcatctgcg tccagtcaat
    23101 ttagaagaaa aaagtttaaa acaaggcatt gagcagttat tgaaggaatt gcagaataaa
    23161 atccagattt cgctaaaatg ggatgtagaa gatgtaaaat taactagttc cattgaggat
    23221 cacttgttcc gaatcgttca agaattgtta tcaaacacat taagacatgc taaggctaat
    23281 gaattagaag tctatttgca taaaatagat aacaatcttt tattacgtat cattgacgac
    23341 gggacaggat ttaatatgaa tgaaaccaaa acgggaagtt atggattgaa caatatcaaa
    23401 gaacgagtag ctggtattgg tggaacagta aaaatcatta gttttaaagg gcaaggtacg
    23461 agcgtggaga tcaaggtccc tttgatgaag gaggcataga taaagatgat aaaagtttta
    23521 ttagtagatg accatgaaat ggtacgctta ggcgtctctt cttatttatc tatccaagaa
    23581 gacattgaag tgattggaga agctgaaaat ggacgtcaag gctatgaaaa ggcgatggca
    23641 cttcgaccag atgtcatttt aatggatcta gtcatggaag aaatggatgg catcgaatca
    23701 acaaaagcaa tcttaaaaga ttgtccaaaa gccaaaatca ttatcgtcac aagttttatt
    23761 gatgatgaaa aagtttatcc tgcaattgaa gcaggagcag caggatattt gttgaagacg
    23821 tctaccgcac atgaaatagc tgatgctata agggctacgc aacgcggcga gcgagtattg
    23881 gaacctgaag tgacaacaaa aatgatggaa aaaatgagtc ggcgaaatga tccagtattg
    23941 catgaagaat taacgaatcg agaaaatgaa attttgatgc tgatttctga agggaaaagt
    24001 aatcaggaaa tcgcggatga attgtttatc acactgaaaa cagttaaaac gcatgtgtcg
    24061 aatatattag ctaagttaga ggtagaagac cgaactcaag cagctatcta tgcgtttaaa
    24121 catggtttgg tgaaataaaa acgatgataa agaggataat caaaagctga gaaaataact
    24181 attatttttc ttggcttttt tacaataatg atgcttgctt ttgtttttgg aaaggctttg
    24241 ttatactgag aaaagaagat actaaaggag gaaaatggtg aaacaaaatt ttgcaattgt
    24301 aggccttgga cgatttggcg gaagcatctg ccgtacactg atcgaagctg gacaagaagt
    24361 actggctatt gacagtaatg aagatcgagt caatgaatat atgaatatag ccactcatgc
    24421 tgttgtggca aatgcacagg atgagatgac acttcgttca ttaggtataa gaaatttcga
    24481 tcatgtaatt gtcgctattg gagaagatat ccaagccagt atcttagtta ctctgatggt
    24541 aaaggaaatg ggcgtaccga atattttagc aaaagcccaa aatgagtacc atgcccgtgt
    24601 actagagaag atcggagcag atcgtgtcgt acaccctgag cgagacatgg ggatacggat
    24661 tgcacataac ttagtatcaa agaatattct agactactta gagctgtcag ataaatattc
    24721 tttagcagaa atccgagtct ccaatcctaa atttttcaac aaaacattag ctgaattaaa
    24781 tttcaggcaa cgttttgact taacagtagt cgccatacga agagtagatc aaacggttat
    24841 cgcttctcct gcagcagatg aatatgttcg cgaaaatgat aatctactgg tgattggaga
    24901 gacaggcgat gtggatattc tagacgataa gatgaatcaa tagaaagaaa agagaaagta
    24961 ggttgttggg atggaattaa cggtaacgcc aaaagcacaa aagtggtttg actcagaaat
    25021 tgatctgcca gaaacttacg gaatacgttt ttttggaaaa gtctacggca aaacagaggt
    25081 tcatgatggt ttttcgattg gaatgtcagt tgaacagcca gaacatcctg taaagaaagt
    25141 ggaaatggat gggacacttt tttttatcga ggaagcagat gaatggttct ttaaaggata
    25201 tgatttactt gttgattatg atcccaacct ggaggagcca acctatcact ttaaaaaaca
    25261 ataaaaaaag agagggtgac acaagtttt
//
LOCUS       DACEKG010000042        25113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEKG010000042 DACEKG010000000
VERSION     DACEKG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.042"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            367..1278
                     /locus_tag="IW664_001596"
     CDS             367..1278
                     /locus_tag="IW664_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0848960.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1495..1932
                     /locus_tag="IW664_001597"
     CDS             1495..1932
                     /locus_tag="IW664_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848961.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2027..2911
                     /locus_tag="IW664_001598"
     CDS             2027..2911
                     /locus_tag="IW664_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0848962.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3135..6302
                     /locus_tag="IW664_001599"
     CDS             3135..6302
                     /locus_tag="IW664_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848963.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6322..6867
                     /locus_tag="IW664_001600"
     CDS             6322..6867
                     /locus_tag="IW664_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0848964.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6895..7350
                     /locus_tag="IW664_001601"
     CDS             6895..7350
                     /locus_tag="IW664_001601"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0848965.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7347..8105
                     /locus_tag="IW664_001602"
     CDS             7347..8105
                     /locus_tag="IW664_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0848966.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8191..8931)
                     /locus_tag="IW664_001603"
     CDS             complement(8191..8931)
                     /locus_tag="IW664_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0848967.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8992..10245
                     /locus_tag="IW664_001604"
     CDS             8992..10245
                     /locus_tag="IW664_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0848968.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10567..10794
                     /locus_tag="IW664_001605"
     CDS             10567..10794
                     /locus_tag="IW664_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0848969.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10917..11243
                     /locus_tag="IW664_001606"
     CDS             10917..11243
                     /locus_tag="IW664_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0848970.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11311..12024
                     /locus_tag="IW664_001607"
     CDS             11311..12024
                     /locus_tag="IW664_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0848971.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12334..13185
                     /locus_tag="IW664_001608"
     CDS             12334..13185
                     /locus_tag="IW664_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0848972.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13306..15171
                     /locus_tag="IW664_001609"
     CDS             13306..15171
                     /locus_tag="IW664_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0848973.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15219..16628
                     /locus_tag="IW664_001610"
     CDS             15219..16628
                     /locus_tag="IW664_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0848974.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            16736..17437
                     /locus_tag="IW664_001611"
     CDS             16736..17437
                     /locus_tag="IW664_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0848975.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            17596..18075
                     /locus_tag="IW664_001612"
                     /pseudo
     CDS             17596..18075
                     /locus_tag="IW664_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            18923..19498
                     /locus_tag="IW664_001613"
     CDS             18923..19498
                     /locus_tag="IW664_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0848976.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            19498..19938
                     /locus_tag="IW664_001614"
     CDS             19498..19938
                     /locus_tag="IW664_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0848977.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            19954..20655
                     /locus_tag="IW664_001615"
     CDS             19954..20655
                     /locus_tag="IW664_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0848978.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21143..21568)
                     /locus_tag="IW664_001616"
     CDS             complement(21143..21568)
                     /locus_tag="IW664_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848979.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            21942..22913
                     /locus_tag="IW664_001617"
     CDS             21942..22913
                     /locus_tag="IW664_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0848980.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            22945..24342
                     /locus_tag="IW664_001618"
     CDS             22945..24342
                     /locus_tag="IW664_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0848981.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            24501..25109
                     /locus_tag="IW664_001619"
     CDS             24501..25109
                     /locus_tag="IW664_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848982.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         8573 a         4132 c         5296 g         7112 t
ORIGIN      
        1 tgtataaaaa tatatcataa taaaaacaac aaaaaaatgt ctggaaaatc gaacataaaa
       61 gtgtctgatt tgaatatccc agcagcggaa gaagaaaaac aaaatcgata acatttgttc
      121 taagaattag ttacgttaaa tacggaacgc aagatagact tgaaaaacgg taacaaaaga
      181 agtagtatat taaggtgtga tgtgctactt gacatttatt ctgctgagcg aactttctgg
      241 attgtgtagc cttatatcta ttcaggacgt atcgagcaga ttagtaaagg tgttcttgat
      301 gattttcatc ttgaacacct ttgtttttag caattcgcaa aaaacgaaaa gtaggagaat
      361 tgataaatga aaaaaagatt tttagcactt gccatcgtac taggcactgg cttattgtca
      421 ggctgtacga atgccggtga aaaaacagcc gttagttata aaggcggtac aatcagcgaa
      481 caagaagtaa tggatagctt gaaaaaaatg caaggagcag attccgctgt gcaacagttg
      541 atcgtttatc aagtatttga agataaatat ggagatgacg tatcaaccaa agaaatcgat
      601 tctcaatatg atcaaacaaa gaaacaattg ggtgattcat ttgattcaca attgaaatct
      661 gcaggttaca cagaacaaac attcaaagac agtatcaaac aaagtctagc ctttcaagaa
      721 ggtctaaaga aacatatcaa attgaccgat gaagatttaa aaacggcttg ggaatctttc
      781 catccagaag tggaagctca aattatccaa gtagcaagtg aagatgatgc aaaagatgtg
      841 aagaaagctg ctgataaggg agacgatttc tctaaattag ccaaagacaa gtcaactgat
      901 actacgacaa aagaagatgg cggaaaagtg aaatttgatt ccactactac aacggttccg
      961 gcagaagtca aagaagcagc ctttaagttg aaagatggtc aagtttctga tgtgatcact
     1021 tctaccaatg cttcaactta tactacggaa tattatgtgg ttaaaatggt gaaaaaccaa
     1081 aacaaaggca acgacatgga taaatacaaa aaagaattga aagaaattgc aacagacaca
     1141 aaattaagtg acagtacctt ccagaataaa gtaattggcg aagtattgaa agacgctaat
     1201 gtaaaaatca aagacaaaga ctttgaaaat gttttgagta cattcacttc agattctagt
     1261 acagcaagta gtaaataagc ggtataaata aaaaatatga actatttaaa tcaataaaac
     1321 tgttcatcta aatcttttta ttagaactaa atcaacagta gtttaattac tacttgattt
     1381 agttcttttt attttaaaaa ttttttttac ttagaatcca gcgaccctag atatttttct
     1441 gtataatctt aaataaagaa aacaatatca aaaaatgcac gtagaagtag ataaatggag
     1501 tggttttgca aatttctttt atttctagaa gttattcacg tatacggatt tgtttttctc
     1561 acaaataaaa aggcaggaag tacaatggat catttgtata taaaatatat tcatcaattt
     1621 tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat tattcaaggc
     1681 cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat tcctctgatc
     1741 gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt gctatttttg
     1801 tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct atcattttct
     1861 tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat gttttatctt
     1921 gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat ttttttcttt
     1981 atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga aaaaagtttt
     2041 attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc aattagcgat
     2101 agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa aagcagggga
     2161 tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt ttgtcatggg
     2221 cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac atcggccaaa
     2281 tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag ggataccgct
     2341 agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat tggatattgg
     2401 aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc ctgaatcaat
     2461 caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt tcatgtcagg
     2521 aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg atggaaagaa
     2581 ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg gcggttttga
     2641 aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt atttaaaaga
     2701 ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag tagatgaatc
     2761 tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag ctgatgagga
     2821 agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga caatcaaagt
     2881 attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag gagttggcgg
     2941 gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca gccttttttt
     3001 tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata catattttaa
     3061 atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca aaacgtcaga
     3121 gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt ttgtttgtct
     3181 tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac gaatcaacgc
     3241 aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt acttctgaaa
     3301 ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat aagacaagtg
     3361 agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg gcaagagctg
     3421 tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag cttctcgcag
     3481 acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg gaattagaga
     3541 tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc acacaccata
     3601 tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga acagctgcac
     3661 atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct tatatgaccg
     3721 atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg acacttgatt
     3781 tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc acattagatg
     3841 gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc gctccgggta
     3901 aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa ctttatggac
     3961 cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt agtggaaaaa
     4021 cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat gatcgggcag
     4081 atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc atgtttgaag
     4141 tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc aatgacaaca
     4201 atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg aatggtggga
     4261 aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat tctagtcaaa
     4321 tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac gatcaacccg
     4381 ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa ggagccggat
     4441 cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt gagaatggta
     4501 cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca ggtgatggca
     4561 aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat gacgcttctg
     4621 gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa tcaaatatcg
     4681 atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat ttaaatttta
     4741 cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat aagaccggca
     4801 tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt gatttccaaa
     4861 acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa ggaaatggag
     4921 attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca ggcgcaacat
     4981 tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta tcgatcaatg
     5041 ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc
     5101 agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag ctcccgatga
     5161 ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc
     5221 ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac
     5281 gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc acgcaaggac
     5341 aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa caaggcggct
     5401 tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa gtgcaagttg
     5461 ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct gccaaaacga
     5521 cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc aatgcagctt
     5581 ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa aaaaatcaag
     5641 ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct atcccagaaa
     5701 atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagcta
     5761 ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa
     5821 gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg aagatcgtag
     5881 tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag atcaaagcaa
     5941 ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg
     6001 atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag cctttcacaa
     6061 atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag gagtatcaat
     6121 tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag
     6181 attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc
     6241 agatagctgg taattactca gcagagataa catggacatt agcagatgcg ccgacagaat
     6301 aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc
     6361 tctttactta tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc
     6421 gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca
     6481 tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac ggtctttaac
     6541 cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac
     6601 ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg
     6661 aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc ggtcactgag
     6721 acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg agggatcacg
     6781 tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt
     6841 tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc agatttgaaa
     6901 ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa
     6961 aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa
     7021 acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat
     7081 tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt
     7141 gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat
     7201 gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt
     7261 gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt
     7321 attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag
     7381 ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta
     7441 cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac gttgttccag
     7501 ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa ccaaatcagg
     7561 gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt
     7621 taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt actggagcac
     7681 aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat gccggttgga
     7741 aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg
     7801 gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa
     7861 tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca gatcaggcag
     7921 tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag
     7981 agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgctg
     8041 tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagaggaag
     8101 cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac tccctgaaat
     8161 ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg
     8221 gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccgttgaa
     8281 aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta
     8341 agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt gttttaaaga
     8401 agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt tcaaaccttt
     8461 gatactttca atatataaaa tttcccgagg atacaggacg agacttccac ctttttgatc
     8521 aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct gttctagcac
     8581 ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg gaaggatttt
     8641 ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa tattactatg
     8701 tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt gcttcaagtc
     8761 gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca actgattgat
     8821 ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca attctctgat
     8881 gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca tatgcaggct
     8941 cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa gatggtcgca
     9001 acagtgacac tttatttgac ctttactttg attttagatt taggtatcta tttattcttt
     9061 tcgaaacaac tgctagtaaa aaacagcttt ggttgtatag ctttgatggt ggtgacactt
     9121 ttacttccta tccctaaagt gagctttcct tacctagttg gcttattatt gatgtttctt
     9181 ttttatttta ctacgaaaaa cctgtttatc agctgtttga tcagtaatct tttttttgct
     9241 atcactggac tcagcttttt tctggttttt gatctgatgg attatttagg aaagagacat
     9301 cttggaacag caagtttttt tgcactactt atcgcattta ttttctatct attagtaaaa
     9361 agtatagaca aacagacgag atttctcaac ttgcttttta cttatccaac tgaacactat
     9421 tttggaggta tttgtatttt attaagctgg ggcgtgattt acttggtatt gggaagtgtt
     9481 cacttccgct catctaatta tctgcttcta tctcttttgt cgatagtcat cagttttttt
     9541 tctttgtttt tttccattat gttgatcagt ttccaagtaa aagaaagaca acatcaggaa
     9601 tcgcttcaac tgtatcaaat aaatgaagaa tattaccatc atctagagga attcaaacat
     9661 gattataaaa gtctactgtt ttcattaaaa attactcttc aggaaaataa aaaagaaacg
     9721 ttggaatttt tagaaatatt agaagatcat tctgcagcta tttttgaaaa accgcaaata
     9781 caacagctaa caaatattgt atccccagct gtcaggggtg tctttttaaa atttctagaa
     9841 aaagcagaat cggagaaaat cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc
     9901 ccgattgatc tggttgtttt tattcgctgt ttatctattt ttatttctaa cgcattcgat
     9961 catcgtgtag ccgatcagag tccgattacg atcaatctgc tggaagaaaa agagggatca
    10021 cgttttagta tagcgaacaa ggcaaatcca acgaatttga cgctatcgga gatggtcaaa
    10081 agaggaaaaa cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg
    10141 gatgtttacg aaaatgcaga actaaaaatc gaattttcaa aacagaatca ctattttttt
    10201 gtagaaatgt atctggcatc tagaaagagc aaatcagcct actgacttac tgtcaaagga
    10261 taagtttgag gaaagtctac acggagatag agacaacaac acagcatatt caaaagaaac
    10321 agaagcgtga tgaccgttgt cacccttctg tttcttttgc tatgtagtta aacaaagtag
    10381 ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact aagtagataa
    10441 gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta caccagataa
    10501 agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc tacgatcaca
    10561 aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat ccatcgaaaa
    10621 gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg aaaatcatta
    10681 agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa acaaaaagcc
    10741 tcaagggaag aagacacgct actgcataca catggtggct acctaaatta ttgattttct
    10801 atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca tctatttttt
    10861 atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga
    10921 atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg
    10981 aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat
    11041 ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag
    11101 atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca
    11161 ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac
    11221 gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa
    11281 aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg
    11341 ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa
    11401 cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca
    11461 aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa
    11521 ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt
    11581 gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat
    11641 acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat
    11701 tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat
    11761 ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca
    11821 ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa
    11881 gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa
    11941 ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc
    12001 aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag
    12061 ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga
    12121 ggtgcatctc tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt
    12181 atatgaaaag gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg
    12241 attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag
    12301 gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac
    12361 aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta
    12421 gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt
    12481 ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag
    12541 gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt
    12601 tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa
    12661 gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa
    12721 tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct
    12781 gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac
    12841 atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa
    12901 ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg
    12961 cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc
    13021 tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag
    13081 tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt
    13141 catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat
    13201 ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa
    13261 tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca
    13321 gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat
    13381 tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta
    13441 aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt
    13501 ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg
    13561 gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt
    13621 atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca
    13681 ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct
    13741 gaagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag
    13801 ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc
    13861 acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca
    13921 ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa
    13981 ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt
    14041 gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt
    14101 gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca
    14161 ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca tcggggattc
    14221 gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt
    14281 cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat
    14341 acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa
    14401 ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct
    14461 ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg
    14521 gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg
    14581 acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt
    14641 ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga
    14701 gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca
    14761 ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt
    14821 gatccagaag taggagaact agttgcacct gcagacggag agatcacgac tatttttcca
    14881 actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg
    14941 atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc
    15001 gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat
    15061 agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta
    15121 gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat
    15181 caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc
    15241 aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg
    15301 gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag
    15361 aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc
    15421 ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa
    15481 tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc
    15541 tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa
    15601 tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct
    15661 atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga
    15721 ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa
    15781 gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa
    15841 gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga
    15901 ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga
    15961 tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg
    16021 catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg
    16081 aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt agttattata
    16141 tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg
    16201 gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag
    16261 ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg
    16321 aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc
    16381 ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag
    16441 atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa
    16501 tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga
    16561 atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa
    16621 acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct
    16681 ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa
    16741 aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg
    16801 aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt
    16861 cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa
    16921 gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc
    16981 ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga
    17041 tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc
    17101 tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta
    17161 tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg
    17221 tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt
    17281 tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat
    17341 acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac
    17401 ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa
    17461 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt
    17521 tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa
    17581 gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct
    17641 tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa
    17701 aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt
    17761 cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca
    17821 acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt
    17881 attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt
    17941 gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta
    18001 gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat
    18061 ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct
    18121 tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt
    18181 ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa
    18241 aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac
    18301 aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga
    18361 aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca
    18421 ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga
    18481 tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa
    18541 caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat
    18601 ttatcagtga aacataacac gagaacaaaa tgattttttc gttaataaaa gaaacaagta
    18661 tttgttgttt aaaataatac acgttattgg catataggac gaaatcccag tatcttctat
    18721 aagtaagtca tttcatatcg atgaaaacac gttatttatt tttgttttat ctataaaatg
    18781 gctgaaaaat gatttttttc taaatactat atatagtggc gtctatccta tataccacta
    18841 aaaaactaca aaatctagtc ttttatttta aaaaaacacg tgtagaatga agatagactt
    18901 ttgagttagg gagtgttttt ttatgaaact tactgttttt tgcggttccc gttttggtaa
    18961 taaagaaagt tacaaattta ttgcgcaaac gttaggaaaa tacatggcac aagaagaaat
    19021 cgaactggtt tacggtggtt catactccgg gattatggga gtgattagta aaacagtact
    19081 ggaaaataac ggaaaagtca caggtatcta tccgaatggg ctttttgaaa ctgaattgcc
    19141 tggaaaagat gttactacat ttattcctac agaaacgatc gatgaaagaa aggtattatt
    19201 atttgaaaag ggagatgctg tcctcgtttt ccctggaggt ctaggtacat tggaggaatt
    19261 ctcacaactt ctttcttgga tagcaatcgg tctgacgccc gacaagccaa tcggaatatt
    19321 gaatattggc ggttattaca gcggcttgca acaattatta gaaacttttg caaaagagga
    19381 ttttatggat aaaaaatggt tggatcaagt aatcttttcc aacaatccat tggaactggt
    19441 caatgaatta cgagcagtat ctattaaaaa tcagttatta ttgaaggagg caaaataatg
    19501 agtcaaatat atttagcagg accatttttt tcagaggagc aaatcgatcg agtaagtcgt
    19561 atcgaaaaag cgttggagga aaacaaaaca gtcacaagtt tctactctcc acgtcatcac
    19621 caagaaagca attatgaact attcagcgct ggatgggcgc aggaagtata cgaaaaagat
    19681 atggaagaac tgacaaatgc agaatttgtt gttgctatcc ttgactttga gcatcaaaca
    19741 atcgatccag gaacggctta cgaattaggt gtggcaacta tgttgaaaaa accgatgatc
    19801 attgtacaag aagagacggt acctacgaac ttgatgatta cacaaagtct ccatacgtat
    19861 ttaaaatcag accaagctgt acgtgaatat gattttgaaa cattgcctgt tgaaacttac
    19921 gtaggcgagt acttatagaa gaggaagggt tgtatgtcag tgattttgat ttcaggaacg
    19981 attggtgcag gtaagagcag tttgacggat atgcttgcaa aagagattga ttctaaacca
    20041 ttttatgaaa atgtcgaaga taacgaagta ttgccgctct tttattcaaa tcctgagcag
    20101 tacgcttttt tattgcaaat cttctttttg aacaagcgat ttttagcaat gaaaaatgct
    20161 ttagtgaacg atgacaatgt gctagaccga tccatctatg aagatagcct gttgttccat
    20221 ctaaacgctg atttaggtcg ggtaacggat atcgaagtac agcagtacga tagcttatta
    20281 gatacgatgc taaacgagtt agatgatgtt gctccaaaaa aacggcctga tttgatggtc
    20341 catatcaaag tttctttgga cacgatgcta gaacgtatca aaaaacgtgg gcgtgactat
    20401 gaacagctag aaagcgatga gacgttatat acctattatg aaacgttgaa cacccgctat
    20461 aatcaatggt atgaagattt tgatgtatgt cctaaaatac aagtggatgg ggataaattc
    20521 gattttgtag aaaatccaga agatgcaaaa aaggtgatcc gtcagatcca agaaaaatta
    20581 gctgaattgg aaggaaagcc tgctggaaaa tattttacgg ataaccagca tgcaaaaggt
    20641 caagaattag gctaaaaata aaaaaccaaa cgacgggaaa gaaaaattac tacgtgatgt
    20701 agaataagcc tttctatcgt ttggttattt tatattcaaa tatttgaaat aaataaatct
    20761 ttactctttg atttgtaaac tgaaatgatc cactgtttgt tttactgatg aagcttgatc
    20821 aaaggaagaa gctgagtttg aagttcataa aaatcagcca tagtgagttt tgcttggatt
    20881 tgtttatctt cttctacttt ttacataatt ttgaacagga taagattcaa atgttttaaa
    20941 tgaatctttg actagtttgt gacagttgtc gcatatgctc aagcttcctt ggtcattaaa
    21001 ttctgtatgg atatttttgt tgttattaca tgtacaataa tcaggactat cgatcatcca
    21061 tttcgtctcc tttacaatca tcttgcttct ttactatagc agaaaagaag gggagaaaca
    21121 aaagaagatc caacagacgg cactatagac tgtagatgtt gccatcctca tccttcaaac
    21181 ggtaattacc gatttttgta aatatgactg ttgtttcttt tttattttct tttcctgaaa
    21241 taatatattc gtcatctttg gtagtgatct tgatatctgt cagcactgtt ttttcttcag
    21301 atgattcaga agaatcagtt ccttgtgtct gatacgtgcg tgtccattcc atttttttat
    21361 ctgtaaatgt caactgtccc gcggataaat ccatatcttt acgtactttt agcgtatata
    21421 cactgttggt ggtatctgct ttttccgctt ttaagacaga agaatcggtt gtttcgtccg
    21481 aggattggtc agagccgcca aacaaatcac agccagtcaa tgtcaaaagt cccgcagaca
    21541 aaataaatag tacgattaat ttcttcaaaa tacttccccc atttgatagg ataaagaaat
    21601 tataccattg gaatgtaaaa cttcaaacgg atggaagaga aaaacgcaca aaacacccac
    21661 aaatagagta aattttagaa taaaatttta aaaaggcaaa aaatccgcca ctttcagaaa
    21721 gcgttttaaa aaagtgttgc aatcttttaa aaaaatgcta tattaaaaat gaaccggttc
    21781 aaatacttgt ttaagtataa ttaatagtga gaggaagttg ctctactgaa gactttagtt
    21841 atatggatat tgatgacaag caacactatg caaagaatgc ggtcatcata atcgaacaac
    21901 aagaaagaga aatcaaatgt aactggcagg agtgagaatg tatggcatcg atccgtgatg
    21961 tggctaagat ggctggtgtt tcagtgggaa cagtttcccg ttacttgaat ggtcagcaac
    22021 tgaaggaaaa gaacatgaaa aaaatagcag aagcgatttc agcgttggac tataaagaaa
    22081 acatcattgc taaagggttg aagaataatc gaagcttctc tattggactt ttgataaacg
    22141 ggatttccag ccgttttggg tcggaagttg tctcagggat cgaaagagta gctgaagaaa
    22201 atgggtatag cttattatta agcgggtttg ccgatcagcc agaaaaaatt gaacaaaaaa
    22261 ttgaatacct gatgaagcat gtgatcgatg ggctgattgt cttcttatcc gaagaagagt
    22321 ggtctggatt tgaaatgctc acaaaaatga gtattccagt tatcgcattg aattgtccag
    22381 aaggaccagc aggtattgat acaatattag tgaatgatcg agaaagtgta gcaaaagtga
    22441 tcgctcatca tggtgaacaa ggacataaaa aaatcggatt tattgctgcc actcaaacag
    22501 actacgtagc aagagaacgt ttagcaggag ccaaagaagc ggtccaaaat gatccatcga
    22561 tccaattaga ggtctttaca ggagactatt ctagaaaaag cggctattac ggggcaaaag
    22621 cattattaga aaaaggggca actgctattt ttgtcagcaa ttacaatatg tcgattggtg
    22681 ccatcgaatg tttcaatcaa gaaggtgtca gaatcgggaa ggatattgcg ttcagtcact
    22741 atgattattt agataaaaac gatctgagta tccttccgaa aatcacgatc acgccaccaa
    22801 cagataaaat cggggaacga gcagcagaac agctgttaaa acgaatcaaa aaagaagagg
    22861 aagaaaatga aaaagtgatt gtgatggaaa atatcataca tggaatcaac taaaacgaac
    22921 taaaaaagaa aaagaggtgc tttgatgaaa aaatatcaat ttccagcaaa tttttggtgg
    22981 ggttctgcag caagtggccc acaaacagaa ggaaggattt caggcgatgg aaaaggagag
    23041 aacatttttg attattggta caaaaaagaa ccgcaaaagt tctttgatca agtaggacct
    23101 gaaaaaacct cgcaagttta tacgaagtat caagaggatg tacagttgat gaaacagact
    23161 gggcataatt catttcgaac atctatccaa tggagccgtt tgatacctga tgaatctgga
    23221 acaatcaacg aagaagccgt tcgtttctat catcgttatt ttgatgagtt gattgaaaac
    23281 ggtatcgaac cctttgtgaa tctgtatcat ttcgatatgc cgatgtatct gcaagaaaaa
    23341 ggcggatggt tgaaccgaga aacggttgaa gcctatgaaa aatatgccaa aagttgtttt
    23401 gaattgtttg gttcgaaagt aaaaaaatgg tttactcata atgaaccaat cgttccagtc
    23461 gaagcaggtt atttatatcg atggcattat ccagaagagt cagatatgaa aaaagcgatc
    23521 caagttggct atcatgaagc gttggcttca gcgttagcca tcaaatctta ccatgaaatg
    23581 gagcaaggtg gtgttattgg tatcatcttg aatctgacac caagctatcc aagagacgaa
    23641 tcaaaccctg aagatgttaa agcagcgcaa atagcagatg cgttcttcaa tcgttcattc
    23701 cttgatccag ctgtaaaagg cgagtttcca agtgaattga ttgagatagt aaaagaactt
    23761 gatctcatgc ctgtgatcca tgcagaggat ttgacggtta tcaaagaaaa tacagtagat
    23821 cttttaggta tcaattatta ccagccacgc cgggtaaaag caaaagaaac accaatcgat
    23881 actaaaaacg gtccaatgcc tgaggattat ttcgacaatt atgaaatgcc tggtcgtaaa
    23941 atgaatcctt atcgcggatg ggagatctat gaacaaggaa tctatgatat tttgatcaat
    24001 gtacgagatc attatggaaa tatcgactgc tttatttctg aaaacggtat gggtgtagaa
    24061 aacgaagcac gatttatcaa agaagatggc atgatccacg atgattatcg gatcgaattt
    24121 gtccaatctc atctgaaata tgtccatcaa gccattcaag aaggtgccaa ttgtttaggc
    24181 tatcatatgt ggacatgtat ggataactgg tcgtggacca atgcatataa gaatcgatat
    24241 ggctttattt cagtagactt agcacaagat ggaaaaagaa caatcaaaaa atcgggtcat
    24301 tggttcaaag acgtatcaga taaaaacgga tttgaggctt aagaaagaat agttgagagc
    24361 aaatgcgtga aattctccat tcttaccatt ttgtgaaaaa gaagttcaat tatttacgat
    24421 ctttttcaac ttttttgtct ttctatttca ttttttgtat ataatagaat agtcaaaatt
    24481 aaataggagt ggaggaaagc atgaggaaaa aaagaccgtt tcttctcttt tggttattcg
    24541 gttttttctt actgcttatt ttatccgcag cattttcagg aaaaatgaca gcagatcttg
    24601 ctcagaaaga aacagaaaaa atcgtagcaa gcaaagaatc agtagataat ttcaaaaaag
    24661 aattctcaac ttattggtgg gcggagtata aaataactgc caattacgaa aaaggcgaat
    24721 tggacattta tttgccaaaa ggaaaagaag aaaatcaatt tattaatatc gatgacgtac
    24781 ttagtgcgat ttctctcaga caatatcaat ttaaagacaa gcatttgacc gttttgcttt
    24841 tgactcatga tggcgacaaa gtagcccgtt tgatcaccgc ttattctgaa aaagtagaca
    24901 aaaaagtggt cgccgatcgt ttagcggaat ggaaaaagac aaatgaacaa gtcaagaaaa
    24961 caactgtctc ttctcaaaaa aacgagtcga caggaacaag tccttcagat tctggcagtg
    25021 gacaaacgat aacgggaagt gaagaaaatc aagcctcaat tattacgaat gagtcatcac
    25081 aaggacaata tcaatcctat ggacaatgaa aaa
//
LOCUS       DACEKG010000043        23658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEKG010000043 DACEKG010000000
VERSION     DACEKG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.044"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            256..531
                     /locus_tag="IW664_001620"
     CDS             256..531
                     /locus_tag="IW664_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0848983.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2622
                     /locus_tag="IW664_001621"
     CDS             544..2622
                     /locus_tag="IW664_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0848984.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2782..4668
                     /locus_tag="IW664_001622"
     CDS             2782..4668
                     /locus_tag="IW664_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0848985.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="IW664_001623"
     CDS             4680..5627
                     /locus_tag="IW664_001623"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0848986.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="IW664_001624"
     CDS             5647..6174
                     /locus_tag="IW664_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0848987.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="IW664_001625"
     CDS             complement(6237..6890)
                     /locus_tag="IW664_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0848988.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="IW664_001626"
     CDS             7022..7864
                     /locus_tag="IW664_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0848989.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="IW664_001627"
     CDS             8022..8918
                     /locus_tag="IW664_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0848990.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="IW664_001628"
     CDS             8921..9634
                     /locus_tag="IW664_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0848991.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="IW664_001629"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="IW664_001629"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0848992.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="IW664_001630"
     CDS             10169..10396
                     /locus_tag="IW664_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0848993.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="IW664_001631"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="IW664_001631"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0848994.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11724)
                     /locus_tag="IW664_001632"
     CDS             complement(11182..11724)
                     /locus_tag="IW664_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0848995.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="IW664_001633"
     CDS             complement(11961..12359)
                     /locus_tag="IW664_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0848996.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="IW664_001634"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="IW664_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0848997.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="IW664_001635"
     CDS             13472..14239
                     /locus_tag="IW664_001635"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0848998.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="IW664_001636"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="IW664_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0848999.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="IW664_001637"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="IW664_001637"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0849000.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="IW664_001638"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="IW664_001638"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0849001.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18895..19800
                     /gene="xerC"
                     /locus_tag="IW664_001639"
     CDS             18895..19800
                     /gene="xerC"
                     /locus_tag="IW664_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0849002.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19837..20385
                     /gene="hslV"
                     /locus_tag="IW664_001640"
     CDS             19837..20385
                     /gene="hslV"
                     /locus_tag="IW664_001640"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0849003.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20399..21799
                     /gene="hslU"
                     /locus_tag="IW664_001641"
     CDS             20399..21799
                     /gene="hslU"
                     /locus_tag="IW664_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0849004.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21817..22608
                     /gene="codY"
                     /locus_tag="IW664_001642"
     CDS             21817..22608
                     /gene="codY"
                     /locus_tag="IW664_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0849005.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22690..23565
                     /locus_tag="IW664_001643"
     CDS             22690..23565
                     /locus_tag="IW664_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0849006.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6404 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcattttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacaccgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt tcttgcgttt ttcttaagaa tttctcaaac aaaatgtgag
    18781 gaattgcgcc aattgtgaac aaacttagaa atattggtta aattctgaca atttatttgt
    18841 ctttttataa acgctgtgct aaattattct ctgggacaaa aggaaggtga gaaaatgaca
    18901 gaagccacgt gggaacaaaa atttttgcgt tatttgattg tcgaaagagg atattcagaa
    18961 aaaacgaaag aagcatatga agaagacatt ctccatttca aacatttttt ggaggaatct
    19021 ggagacgctg atctgctgaa ggcggaatat tttacggttc gtgtctattt gagtgcatta
    19081 gctgatcatc gtcctagcta tagcagaaat tctatcagtc gcaagatagc cagcctacgt
    19141 tcgttttatc agtatcttct caaagaagaa gtgataaaag aaaatccatt ttcttacgtt
    19201 catctcaaaa agaaaaattt gagactgcca cgtttttttt atgaaaatga aatgcaagca
    19261 ctttttgaca gcgtagcagg agatagtcct ttggatttga ggaaccgcgc tttactggaa
    19321 gtcctttacg ggtcaggcat ccgtttaagt gagtgtagca gtttgtcggt atcagacatt
    19381 gattttgatt cagaggtcat gttgatccat ggtaaaggga acaaagagag atatgctcct
    19441 ctaggttctt ttgctcaaga tgctctgcaa gaatatttca gggaaggccg ccaggtattg
    19501 atgacaaaat atcacgaaga gcatgatatc gtttttgtga atcatcatgg aaaaccaatc
    19561 acgcctactg gaattgaata tgtcttgaat caaatgatca aaaaaagcag tttggatagt
    19621 gatatccatc ctcacatgct gcgccataca tttgctactc atttattaaa taatggggca
    19681 gatttgcgga cagtccaaga attgctagga catgccaatt tatcgactac ccagatctat
    19741 gcccatgtaa caaaagaaag tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa
    19801 aaataaggaa aagaacaaat ttaggaggaa acaaacatgg tcgaatcacc atttcattca
    19861 accactatct gtgccgtcga aaaagacggg aaatttgcta tggctggaga tggccaagta
    19921 acaatgggtg aatctgttgt catgaaaggc actgcaaaga aagtacgacg tatctacaat
    19981 ggagaagttg ttgtaggttt cgccggaagt gttgcagatg cgtttacttt agaagaaaaa
    20041 tttgaaggca aactaaacga atataacggg aatctacaac gcgcagctgt cgaattagct
    20101 caagaatggc ggacacagca atctatgcaa aaacttgaag cgatgctgat cgtaatgaat
    20161 gataaagaaa tgcttcttgt ttcaggtaca ggagaagtta tcacaccaga tgatggtatc
    20221 ttagctatcg gatcaggagg gaattttgcg ttggctgcag cccgagcaat gaaaaactat
    20281 gctcaaaacg acatgagtgc gaaagaaatt gcagaaaatg cgttaaatat tgccgcagat
    20341 atctgtatct ttactaatca taatattatc gtagaagaac tgtaagaggt gagtagatat
    20401 gtatgaatta aaacaaacac caagacaaat cgtaagtgaa ttagatgaat atatcgtagg
    20461 acagcacgca gcaaaaaaat ctgttgccgt tgctttgcgt aaccgctatc gccgtttgca
    20521 gttagatgaa aaaatgcaac aagatattac accaaaaaat atgctgatga tcggacctac
    20581 aggggtcggt aaaacagaaa tcgcacgacg tttggcaaaa atcgtcaatg caccatttgt
    20641 caaagtcgaa gcaacaaaat ttactgaagt cggttatgtc ggaagagatg tagaatcaat
    20701 ggttcgggat ttagtagaaa atgcgatcca aatcgtagaa aaagagcagt acagccgtgt
    20761 atacacaagt gctaaaaaaa atgcagaaaa acgtttggta aaaatcttgg tacctggtat
    20821 caaaaaagaa cagaaacaat ccactaatca atttgaacaa atgatgaatc tttttaatgc
    20881 tagtcagcaa aataccgctg aaccgcaaga agaagtgaca gaagatattc gaatcaatcg
    20941 ccgtacagtc ttggaacaac tacaaaaggg tttactggat agtcgtgaag taaccattga
    21001 agttgaagac caaaagaaac ctgcaccaat gatgaacaat ggattggagc aaatggggat
    21061 cgacctaaat gaaactctag gtgctttgac accgaagaaa aaagtggaac ggacggtaac
    21121 agtcaaagaa gctttagagt tattgatcaa ggaagaatca gcaaaattag tcaaggatgc
    21181 tgatatccat agtgaggcaa tcaaattagc ggaagaaagt ggaattattt ttatcgatga
    21241 aatcgataag atcacttcta aatcacagca atctggagaa gtatccagag aaggcgtcca
    21301 aagagacata ttgccaattg tcgaaggttc acaagtaaac acaaaatacg ggatcatcca
    21361 aacagatcac gtcctattca ttgcttcagg agctttccat ttgtctaaac caagtgatct
    21421 aattccagaa cttcaaggac gcttcccgat acgggtagaa ttagatgatt tgacagctga
    21481 agattttgtg aagattctga ccgaaccaaa taatgcttta gtaaaacaat acgtcgcgtt
    21541 gatcggtaca gaaaatgtgt cagttacgtt tacaaaagaa gcaattgaac ggatcgctca
    21601 aatcgctttc gatgtcaacc gtgacacaga taatatcggc gcacgcagac tgcataccat
    21661 tttggaaaaa ctactagaag atctattatt tgaagcacca gatatgcaaa tgggagaaat
    21721 aacgatcaca gaagcatatg tggatgacaa acttggctca attgcggcaa atgaagattt
    21781 aagtcgctat attttataaa aaatcggagg acgacaatga cagacttgct aacgaaaacc
    21841 cgcaagataa acacactttt gcagcaaaag aatacttttg acatgcaagc agatttgcct
    21901 tacgataaaa tggctgtcac actaggtggt attcttgaca gcaacaccta tatcatagga
    21961 agtgacggag tattactagg ttataatgaa cgtcacgatg tgaacaatga acgaatcaaa
    22021 acaatgtttg ttgagaaaca atttcctgaa tcttatactg atacagtggc acagttgatg
    22081 aaaactgaag ctaatatccc aattactagt gatattactg catttccggt agagtcaaga
    22141 gagaaatatc catttggtct gacgactgtc gttccgatct ttggagctgg cgaacgtttg
    22201 ggtacgatta tcttatctcg tatggaacaa tcgtttgcag atgacgatct agtcttagca
    22261 gaatatggag ctacggttgt cggtatgcag atcctttatc agaagtctcg aagcatcgaa
    22321 gcagatgtac ggagtgcaac ggctgtccaa atggcaatca acacattatc ttatagcgaa
    22381 ttgaaggctg tacaagctat ctttgaagca ttggatggag atgaaggtcg tctgactgct
    22441 tctaatattg ccgatagtat cggcatcact cgttctgtga tcgtcaatgc tttacgaaaa
    22501 ttggaatcag ctggaatcat tgaatctcgt tcgttaggca tgaagggaac ttatttaaaa
    22561 gtattgaatt cccgttttaa agacgagtta gcaaaacaca gttattagaa gtaaggggac
    22621 tggggcagat agaaatgttt gttccagcct tcccatttta aaaagtaagt cgaagttgga
    22681 ggaatggcaa tgactgttat tattcaaaat gaccagctga tcgccgaaat cgctgaacac
    22741 ggtgcggaat tgatcagtct gaaatcaaaa gaacatggtt tagagtatat ctggcaaggt
    22801 gatccagcgt attggggcag acatgcaccg gttttattcc cttttgttgg acgtttaaaa
    22861 gatgatcagt atgtttatca agggaaaaca tatcaaatgg ggcaacacgg atttgctcgg
    22921 gatatggatt ttgaagtcat tgaacatgga caagaaatgg ctagtttcat cttaaggagt
    22981 acgccggaga cattagaaaa gtatccattt gattttgaat tagtgatcag ctatgaatta
    23041 ggaggagacg gcatcacggt tcattatcaa gtggcaaata ccggtgaaaa agaaatgttc
    23101 ttttctatcg gtggacatcc tgctttcaat gtacctcttg aagaaggatt gtcatttgaa
    23161 gatttctatc tggcattttc tccattgaaa tcaagattac gcctaccatt gaaaggccca
    23221 ttcatcgata tggatcaaaa gacgttaggt cagacaaata cgaatctggc tttgaatcgt
    23281 gaactttttg agcaagatgc cttggttttt gaaacaaggg gtctaaatgc gtacgccatt
    23341 cgaagtgaaa aatcttccca tagcgtaact ttaagctata aagatatgcc ttatgtaggg
    23401 atctggtctc cttatccaaa agaagcgcct ttcgtttgta tcgaaccgtg gtgcggaatc
    23461 gctgacacgg tgaacagtag tggaaacttg atggaaaaag tagggatcaa tcaactaggt
    23521 gcccatgaaa tcttcaagac aaaatattcc atcacagtga agtaagaggt tgtgaccgag
    23581 aagttagcag ggaacaccgt ttaccaaggt gtgtaaaaaa gctatcctgt aagtaataag
    23641 ggagtgtgac atcgattt
//
LOCUS       DACEKG010000044        22339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEKG010000044 DACEKG010000000
VERSION     DACEKG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.045"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..156
                     /locus_tag="IW664_001644"
     CDS             <1..156
                     /locus_tag="IW664_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0849007.1"
                     /translation="RKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQM
                     LSQPMEN"
     gene            534..2576
                     /locus_tag="IW664_001645"
     CDS             534..2576
                     /locus_tag="IW664_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0849008.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2587..3207
                     /locus_tag="IW664_001646"
     CDS             2587..3207
                     /locus_tag="IW664_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0849009.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3219..3677
                     /locus_tag="IW664_001647"
     CDS             3219..3677
                     /locus_tag="IW664_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0849010.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3696..3974
                     /locus_tag="IW664_001648"
     CDS             3696..3974
                     /locus_tag="IW664_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0849011.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3999..5366
                     /locus_tag="IW664_001649"
     CDS             3999..5366
                     /locus_tag="IW664_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0849012.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5381..6526
                     /gene="dgoD"
                     /locus_tag="IW664_001650"
     CDS             5381..6526
                     /gene="dgoD"
                     /locus_tag="IW664_001650"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ0849013.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6553..7044
                     /locus_tag="IW664_001651"
     CDS             6553..7044
                     /locus_tag="IW664_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849014.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7487..8686
                     /locus_tag="IW664_001652"
     CDS             7487..8686
                     /locus_tag="IW664_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0849015.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9181..9939)
                     /locus_tag="IW664_001653"
     CDS             complement(9181..9939)
                     /locus_tag="IW664_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0849016.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10107..11042
                     /gene="pfkB"
                     /locus_tag="IW664_001654"
     CDS             10107..11042
                     /gene="pfkB"
                     /locus_tag="IW664_001654"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0849017.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11039..12484
                     /locus_tag="IW664_001655"
     CDS             11039..12484
                     /locus_tag="IW664_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0849018.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12512..12967
                     /locus_tag="IW664_001656"
     CDS             12512..12967
                     /locus_tag="IW664_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0849019.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12986..13960
                     /gene="lacD"
                     /locus_tag="IW664_001657"
     CDS             12986..13960
                     /gene="lacD"
                     /locus_tag="IW664_001657"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0849020.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14071..14632)
                     /locus_tag="IW664_001658"
                     /pseudo
     CDS             complement(14071..14632)
                     /locus_tag="IW664_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14776..15390
                     /locus_tag="IW664_001659"
     CDS             14776..15390
                     /locus_tag="IW664_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0849021.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15631..16014
                     /locus_tag="IW664_001660"
     CDS             15631..16014
                     /locus_tag="IW664_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0849022.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16377..16886
                     /locus_tag="IW664_001661"
     CDS             16377..16886
                     /locus_tag="IW664_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ0849023.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16986..17636
                     /locus_tag="IW664_001662"
     CDS             16986..17636
                     /locus_tag="IW664_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849024.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            18087..19025
                     /locus_tag="IW664_001663"
     CDS             18087..19025
                     /locus_tag="IW664_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0849025.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            19038..20090
                     /locus_tag="IW664_001664"
     CDS             19038..20090
                     /locus_tag="IW664_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0849026.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            20356..20991
                     /locus_tag="IW664_001665"
     CDS             20356..20991
                     /locus_tag="IW664_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0849027.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            21182..21973
                     /gene="msrA"
                     /locus_tag="IW664_001666"
     CDS             21182..21973
                     /gene="msrA"
                     /locus_tag="IW664_001666"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0849028.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7120 a         3547 c         4702 g         6970 t
ORIGIN      
        1 cgcaaggaac agttccaaaa tgaagagtcg atggaacgtt tcttagtctc gtcttttgat
       61 acttacaacc aaaaattcct aggtcgcagt cataaaggtt tccaacaggc cgaaggcgag
      121 ctcgaacaaa tgctgagtca accgatggag aattagaaac aatctacagt agggaagaac
      181 catttacaca aaattattga cgctcccaaa agcattgttt ttagcagttt aatattattc
      241 tttttcaatt agactgactt gatttagtta cggaatagat actttaatgt gttgaagaag
      301 caacgtcccg tgtttattta ctaaagcatc caatattcta ccgaatattt aatattcaca
      361 ttagccctaa aataattgtc ataccatgtt acatttgata taacagcgtt cattttaaaa
      421 atagctgtta gtgtgacaaa acattaaacc aagtgtgaca aaaaacgtga tgaaagcttt
      481 ccggaaacgt atgtgaacaa gtatataatg attttgtcag gaggatagtc aaaatgaata
      541 aacgaattcg tgaaatactc aaactgatta ttaaaaaccc tgaaatgaaa ctttccactt
      601 taacaagtga acttgatttg acacgtcgtc aaatcaatta cgcgatcaat caatttaatg
      661 aagaattgaa aacaaagaac attccgttaa ttcagagaag tcattctgga aatattacgg
      721 taccaatcga ggttatccaa atgatatcac agcctgatta cgacataggt gatgatcagg
      781 aacttgtcgt tctcactgat agtgaaagac aagcattgat agtgatgact ctgatcgcta
      841 atatcgatta catcagtctg gatcatttgg tagatgcggt tgatgttagt aagacgactg
      901 ttatggaaga tatcaaacga acggaaccgt tcatcagaaa ttatagtctg acaattcgat
      961 ataatcggat gaatggttac caattggttg gttcggaaca ccgtatctta cagctgttat
     1021 cggatctagt aaaacggttt ccgatttttc gccgagaaat gattcgcaga aaattggcac
     1081 aagatgtttc tgaagaagaa atcgtccatt tgatccataa catggaacaa atgttgcatc
     1141 ttagttattc tgatgagtcg gtagactatt tgcagacatc agcgagattt ttagtaagcc
     1201 gtgggatcaa gatgtcagat aagcacgcgt tttttcccga tgatgtgacg gatactcctg
     1261 aattccgaat gctgatgatc ttggtacatg aaacaggttg gagtctatcc aaaagttata
     1321 tggagtggtt cacgttatta tttttaactt ccaatatttt tgagagcaaa actactcaaa
     1381 attatgattc ggatcaagaa ttgcgaaaat tgatcagcca aatggttgaa aatttccaaa
     1441 atcaaacgtt gattttgatc gatgaccgag aagactttga acgtaggata ctgagtcatc
     1501 tgcgtccagc ctgttttcga atacgctata acttgagttt aggagtttat tcaatagata
     1561 gtctgatcaa ggatagtaat catgcgattt tgattgattt gatgaaagaa ttgattattc
     1621 ctattgagaa ttggctaaat aaggcctttc caaatgatga attagatctt ttatcctatt
     1681 actttggcta tcaattaagt aatcataata aattgaataa gcaacgacaa agggctgtag
     1741 ttgtttgtac taatggtgtc atggtctcaa agttagtacg agccaatatg gaaaaacttt
     1801 ttccagagat ccatttttta gcttccctat ctgtgcgtga tttctacaaa tttgaagctg
     1861 attatgatct ggtctttact acgacgccac taaaaagttc tatgctgcag tttatgatcg
     1921 agccaatcat gacatatcag caacaaatca gtttacgata tcgagttttg agtgatttag
     1981 gaattactaa ggttgatcga gcaatggatg aattgttaac aatcattagg aaatatgcaa
     2041 atatcaccaa tccagtttcg ttgcgagaag aactgcagta ctttttgatc aaggaagatg
     2101 gaagcacgcc gctggacaac ttcaatgtat tgccttcatt gacttattac ttgcggccga
     2161 gttatgtaca aatcgttgaa gaagagatgt cttgggaacg agcagtggag gtggcttgcc
     2221 gaccgttatt ggaacatcaa atcattgact cgcgttttat cgctgattgc ttggatcaaa
     2281 tcagacaacc gggatatgca ggttacttgg gaatgaagac ttgcattccc catacaacgg
     2341 tcgataaagg tgtaatcagt gacggtgtca gcattttagt ttgtaaaaag ccgatacgat
     2401 ttcctaatgg tgaagatgtc agcttgattt tgccgctttc attttttgat ttaacgaagc
     2461 atctgcgagc catcaatcaa atcgctgata tcgccaaaga tgattcattg attgagcagt
     2521 tattgattgc tgatgacgaa aagacagttt atcaactatt aagacaaaga tcttaaggag
     2581 gaaaacatga gactagcaga ttatcctaaa ttgacagtga tcatgcgtgg ttatactttt
     2641 gatcaggcag atgctatttt acaagcaatg caggaatttg accatcaatt tgccgtagag
     2701 atgacaatga acacaaaagg agctcttgaa aatatacatg aactaaatca acgttatggt
     2761 gacacgactt atatcggcgc tgggacggta aggacattag aacaagccca agcctgctat
     2821 gaggctggtg caaaattttt attaggacca catatgttca caaaagaaat gttgtcttac
     2881 gccaataaga aaggattatt ggcggtacct gccgcaatga ctccttcaga agtcgaccgt
     2941 atgtttaaag aaggagctga tattgtaaaa gtatttcctg cagcggttgt tactccacgt
     3001 tttttcaagg atattcaagc acctttaggt aagttaccac taatgggggt tggtggaatc
     3061 agtaaggaga atgcgaaaga gttttttgaa caaggtgcta gctatttagg tcttggttcc
     3121 ggtatgttca acaagcaaga catcgaagag ctaaatgtta aaaacttagc acgttcgatg
     3181 aaagaattaa ttgaggctat taaataggag tgaggacttt ggaactttat ttgaatgagg
     3241 aactcgtttt tcgaaatgtt caagcaaaaa cagatagtga agtcttagcg tttttagcaa
     3301 gcgagatgta caaaaaaaac tacgtgaagg aggaatatat tcaagcaatc caagaacggg
     3361 aaaaagaata ccctactgga ttaccatcaa caccaccagg aatcgcaatt ccccatgcaa
     3421 actatgaaat ggtcaataaa acaactttag caattgcgac tcttaaagat ccggtcctat
     3481 ttcataatat ggaagataac aatgagcagt taccaatcca aattgtgatc atgatggcga
     3541 ttggggaacc acatgggcaa gttgaaatgt tacaaaaaat tgttggaatc atacaagatg
     3601 agccactgcg gcaagaaatg atcagagcag gcaatgatac tgaattatta gaactattaa
     3661 agaaagcggt tttttaatat aaggaggaaa tcataatgaa acgaatttta gtagcatgcg
     3721 gaaatggtat cgccacttca acagtagtag caacaaaagt tagagagtat ttgcaacaac
     3781 atggcattga tgtttcaacg actcaaacaa aattgatgga agttccagga aaagttcaag
     3841 actatgatct attggtgacg actggtcaat ttgatggtca aacaggtggt gtaccagtaa
     3901 tcaaagggat gccgatttta acaggtatcg gtgccgatca gacaatggaa gagattttga
     3961 acttactgaa ataatagctg atcaaaggag ctgaaaaaat gaaaatcatt atgaacgtct
     4021 ttaacttctt tattgatgct ggaccaacag ttatgttgcc agtcatcatc acgattattg
     4081 gtttgatttt tgggttaaag atttctcgtg cttttaaatc aggtttgaca ttaggtatcg
     4141 gttttgccgg gatcaagttg atattggact ttatgacgac caatgttgga ccagctgcca
     4201 aggcgatggt tgatcgaact ggtgttaaac tagatgcttt ggatgttggc tggggttcga
     4261 ttgcggctgt tacttgggca tcgccaatca ttccaatctt gatttttgcc attctattgg
     4321 tcaatatcgt tttgctgatt ttgaaacgta cacatacgct ggatgttgat atctggaact
     4381 atcaccatat ggcaatcgtt ggtgttatag tgtattttgt gactaagaat gtcttcttag
     4441 gtgtaggtgc atcagtagtg atggcaattg cgacttttaa gatttcagac tggtcgcaac
     4501 cgatggtaga aagtttcttc ggaatccctg gtgtttcttt accaactgtt tctgcgcttt
     4561 catcactggt tattgcttgg ccgttaaatt ggctgctgga tcgtatcccg ttattcagaa
     4621 aatcaaaatt caccatcaaa gatgcacaaa aatatttggg attttttggt gattctatga
     4681 tcatggggtt agttattggg atggtgattg gtgttttagc cggctatgat atcaaagcgg
     4741 ttttacaatt aggtgtcagc atgtcagctg ttttggtttt gattcctaag atgacttcat
     4801 tatttatgga aggattgatg ccaatttcag atgctgcaca aaaatggtca caaaaaaaat
     4861 tcaaaggtac acgtttattt atcggattgg atgctgcagt tgttgtaggt aatccagatg
     4921 ttatcacaac agcattgatc attattccac taacgattgc attagcttta gtattgccag
     4981 gaaaccgtgt gttaccattc gcagatttag cggttgttcc tttccgcgtg gcaatggtcg
     5041 ttgccttgac tcgtggtaat ttattaaaaa atatcattat tggcttagtt gtgacagctt
     5101 cattgctatg gtgtgggtca gctactgcgc caatgctgac agcaattgcc aaatcggttg
     5161 gtattaactt aggaacttct tcaatgttaa tctcatcatt tgctgcgact tccatgatcc
     5221 aaagttattt ggtatttatc gcatttgctt acaaaccaat catcggtatt ccagtgttgt
     5281 tattagcctt tgcgggcgca tggtattact tcgatggtat caaaaaagtt aatcatgaaa
     5341 tggatgaacc attgaaggca caatagtagg agggtttaaa ttgaaaattt cagatgtcac
     5401 agtgtataaa gtaaaaccaa gatggatttt tgttaagatc tcgactgacg aaggaatcga
     5461 aggctgggga gaaatgatct caggaactaa aacagaaacg gttgttgctg gagcttatga
     5521 gataggaaac cggctgattg gacgtaatcc gtttgaaatc gagcgcctct tccaagaaat
     5581 gcatcgatct ttcttccgag gcggtcctat taatggaacc atcgtttcgg gtttagaaat
     5641 ggctctgtgg gatatcaaag gaaaagcgtt taatgttccg gtttatgaat tattgggcgg
     5701 tgcagcacgt gataaaatca aggtttattc ttggattggt ggagatcgac caagtgaagt
     5761 tgcagaacaa gcacaagatc ggtttgatcg gggctttacg gccgtcaaga tgaacgcgac
     5821 agaagagctg cattatatcg attcctacca aaaagttgat gaagtggtgg aacgagttgc
     5881 gtctattcgc gaacgttttg gtgatgcaat ggatattggt gttgattttc atggacgggt
     5941 ccataaaccg atggcaaaag tgctagcaaa agctttggag ccattccatc caatgttttt
     6001 ggaagaagtc gttttacctg aaaatgaaga acactttaaa gaagtagctg attctgtggc
     6061 tgtaccatta gctactggtg aacgcttgta cactcgttgg caattcaaga acctctttaa
     6121 acaaggtact gttgatatta tccaaccaga tgtggctctt tgtgggggaa tcttggaaac
     6181 ccgtaaaatc gcagcgatgg cggaagctta cgacatggct gttgcgcctc atgcgccata
     6241 tggtcctgtt gcactagcag caactctgca agtagactca tgtacgccaa acgtctttat
     6301 ccaagaacaa agcttaggga tccactataa caaaggtttt gatctgttag attttgtgaa
     6361 gaacaaagaa gttttccaat ataaagacgg ctacgtagat ctaccttcaa aaccaggtct
     6421 gggcttggaa atggatgaag acaaaatcaa agaaattgca caagaaggtt tgatttggac
     6481 caatccgcaa tggaaaaatt atgacggtac gatcgctgag tggtgataaa tgaaaataaa
     6541 ggatgatgaa agatggcaga agcacttatg atcacagttt ttttaggact gctgattatt
     6601 ttgcaaataa tcgatcaaaa gaaaattatc gtgtttgctc agagtagtcc tgtaaggaag
     6661 agtttgagta ttttactgac agtcgccatc ctgttgattt tctggccgga acatctgact
     6721 gatcagatta aactggttgc ttttgcttct gtcattttga tcaatggttt tttgaaggag
     6781 ggtctagcaa aagattctgt tgttagattt ggagtattga acggtaactt taatcgatat
     6841 cctcaagtcc aagtagaaaa actaggtgac gataaatcat ttatttcatt ttacaaaagc
     6901 aagaataatt ctttttcaat gatttttgat aaagatcaaa ctgaaatgat ggatgtcttt
     6961 caagaacttg atatccattc caaaattact ttagatcaaa gttcggaaga aaaaagagca
     7021 gttgtaaaaa aagataatga atgattctac taccgaaatt atgatttctt tatcacgaat
     7081 aatgaaactt gttttaatga atcatattga ttgaagtata caatgtatat taagttttgg
     7141 aaagaaatat attggtacaa gatatagcag gaatcgatta ctcgatccct gcttttttta
     7201 acttcctttg cagcaaaaaa agctcgtaat ctaagagatg atggatagtt tttagaatat
     7261 atatattttc ttcaattcca gaaaaaaatg accaagctag tatgctaaaa aagtacgatg
     7321 cttctatgat cttttttccg tctttattaa cagatgtttt aattgatcaa cgcatattgt
     7381 aatctaccct aatttccata gttcttcacc acttttaaaa atagtaaaac ccctacaagt
     7441 atgctatagt aaattgctaa agaaaaacca ataaggtggg agatgtgtgc gcgatcaaac
     7501 aagcggaaaa atgattcgtt tttattatac aagtttgcat tttctttatt ggatgatgtt
     7561 ttgttctgtc tatgggtttg cgacattgtt tttgatttat ttcaagattg atccagctaa
     7621 aatcggaggg atcttagcat tggtcaatat tgtttcaacg gtgcttcagc cttttgtcag
     7681 tcaagtgatc atcaagaaaa gaaagatccc actgatcacc gtgctgaaga cgatgatagc
     7741 aggattagga gtgattctct tagctggggt attgattccg aatttgccga tcattactta
     7801 tatcttgggt gggattttag tagtttctat gcaatcattt ctcaatgcgc tggcatttga
     7861 gtattcaaat accggctaca ggatcaactt tggcttttca agagctggtg gttcattttc
     7921 ttatgctgta acttcttttt tactgggaca gctgttagcg atttattctg ccgcgatttt
     7981 gccaatcttg gttatagttg aagcagtgat atttttcgtt attttgcatc tgctgccgga
     8041 tgtaaaaaat caagaagttt ttcctatgac aaaagaacag gatcgctcag tttctttgag
     8101 aaaaaaatat cctttcctga ttttgttatt tttaggtttc agttttcttt ttactttcca
     8161 caccatgatc aacagctttt tggcacagat ctttgaaaat atccacggcg gcagtaaaga
     8221 agttggcatc gcattgatga tcgccgcttt atgcgaatta ccgggaatga tcttttttga
     8281 acagcttgtc aaaaagaaat ccagcaaatt ttggctgtct gttgccagta tttcatttgc
     8341 tgtcagagga ttcatgatgc tgctggccag ttcgatcctg atgatggaaa tgacgcatct
     8401 cctacaagcc ttcagtttcg ctttgtttat tccagcttcg gcttatttgt tcaatcaatt
     8461 tctggcggat gatgatcgtg tatttggaca aacgatgatc atcatcgcca caactcttgg
     8521 tggggtgata ggaaatgtct tgggcggcag tctgctgcag catttcggtg tatgggagat
     8581 gctgattttc ggtactggtt ttgcggtgct aggtgcttta ttaactattt tgggcattcg
     8641 aaaaataccg aagacaggaa acgatagtga ttctcttgtt gagtagcata aaaatactta
     8701 gatactagtt ttatataaat aacatatagt gattattggc gtaattacaa aaaacgagaa
     8761 taacttaagt aagcatttct aaataattga gaaacctcat ctaaaaagat aagaaaatat
     8821 ccctaataaa acttctgtaa cattttagat aactgattgt aatctgaata ataaactata
     8881 aggagacggt tagacggtct cctttttttg cgtattataa ttctagaatt gttggaaatt
     8941 gataataatt aacaagaaat attagacagg aacttcgaac tgcttatcac attcttatta
     9001 ttatatattt gcgtaagtag tcctggtagt ctcaatgcac tattctatct tgaaactaag
     9061 aagttataga aggagcaatg atagaaagca cgagaataca aatagcgatt aaatattttt
     9121 ctagacaacc ttagttaatt cacacagata tattttaggt acagtcttac agatatagag
     9181 ttagtgctgt gtgaatcctg atgttcgcag tgcataggag tcaagtaaga ttctagccag
     9241 tatagaaagt ggaagtctgg agcgtacctc ataatctggg aagaaggagc cttctgattt
     9301 aaagccaaca aaaggaatct cgatatgttc catcaaactt gaatgacgat tagcagtaag
     9361 caaaattgtc ccgatctcag ctttataaca gttttcagct gcggtagtca attcctttgt
     9421 ttcaccgttt agtgaaacaa agatcacgat gtcatctttg gataattttc tactaatgtt
     9481 ttttatgata ttgggatcag tatgcatctc acaatattta ccggtaagtt gaaatttgac
     9541 catcatttcc tgagcaatca attctgaaaa acctcgggca aaaatcatga ttcttctagc
     9601 agccttgatt cgttgaatag catcttcaat attcccgctt tctaccatat tgatggtgcg
     9661 gattacctct tgctcatttt taagaatagc agtctttatt ccttcatcaa ctttatcaag
     9721 agcggcaaaa ttgatatttg tattttcttt ttcttttaac gaatgtttaa aagacgtaaa
     9781 cccctcatat ccttttttct tcatagtacg aacgacggtt gctgttgaaa cgttggcata
     9841 ttcacttagt tttacaatgg aataggaggg gatttcgagt atgtgacttt gaataaaatc
     9901 ccagagatat ttctccgatt cacttaattt tgtatccata ttttgcctcc gattagttag
     9961 gataattcta ttgtaaagcg ggagtttcct ttacttcaag tttatttaat atattttgta
    10021 aaagttttca atgaatttag tcatttatga aaacatttac attcgatctt gatacataat
    10081 gaatatgtaa gaaaggagga gcgtaaatgt cagtttatac atgtacttta aacttggcaa
    10141 tcgacctttt tatcgaaaca gatgaaatga aaccatccca agtcaatcga acaaacgatg
    10201 atgatataca agctaatgga aaaggtgtga atgtttcatt gatcatgaag caattaggta
    10261 tcgatagtac agctttagga tttaaagcag gttttaccgg gaattatatt gaggattatt
    10321 tgcatcaaaa agatattagc actgatttta ttagcgtacc gggaatgact agaatcaacg
    10381 tttttacaca agttaataaa accaatgaag aattcaaatt ggttaataaa ggacctgaga
    10441 ttcctcagga acaaatcaaa acttttctaa atcaaataca aaagctgcaa gctgacgatt
    10501 atttgtgtgt ttctggcagt ttgccgcgag gggtctcacc cgaaattctt attgatatca
    10561 gtcgtatttg ttttgaaaat aaggtccatc tgattattga ttcaagctat ccggaaataa
    10621 ttgattgtct gccgtatcag ccgttcttat taaaaccaaa cgaggaggaa ttgcaatctt
    10681 ggtataatgt cgagcttagt gatcgtgaag gatatattca ttatgctgaa cgcttgattg
    10741 ccgatggagc gaaaaatgtt ctcctttctc taggaggaga gggaggaata tttttaaata
    10801 aggatatcgt ttattctggg aattctccta aaggtaaagt agtcaataca gcgtgtgctg
    10861 gtgacacttt gttgggaaca tttcttgcag gatatttaaa aggtgaacag gtagatatta
    10921 atttgaagaa aagtttagca gctggaagtt caaccgcttt tcgtaaagga ttaacagatt
    10981 tttcggatgt tgcagagttg gaaaaacaaa tcgaaatcaa agagggagcg agtaaaaaat
    11041 gacaacttac aaaattgttg ccgcaacagg atgtccaaca ggaattgccc acacttatat
    11101 ggctcaagaa gcattggagc aggcagctaa gcgcaaaggg gtatctatca aagttgaaac
    11161 ccatggtcaa gtagggatag agaatgaatt aacaccagaa gaaataaaaa acgctgacgc
    11221 tgtaatcatt gcagctgaca aagatgttca agcagaacgt ttcgcaggta agcgtgtaat
    11281 cgatgtgcct gtcagcaagg ggattaaaga tgctgaccaa ttgatcgaag atgcgttggc
    11341 tggtaaaggt tcaatcactg cagaaaacaa aacagttgac gctgctggtg agaaagcgga
    11401 gatgggggtt gcagcaggca ttggtcacag tatttataaa aatctcatga acggtgtttc
    11461 tcatatgctg ccgtttgtag tggcaggtgg ggtaattatt gcaatttcgt ttgccatctg
    11521 gggtgtgtac tcttttgatc ccaatgatcc tacttacaat ccgactgccg ctatgttaaa
    11581 gggtgttggt gatgcggcaa tgggaatgat ggttcctatt ttagcagctt atatcgctga
    11641 aggaatcgct aaacgtcctg gtttggttgt tgggtttgtt ggcggactgt tagccaattc
    11701 tggcggagcc ggtttcttag gcggaatttt tgcaggtttc ttagcaggct actttatctt
    11761 actattgcaa aaggtcatga aaggcttgcc taaacaattg gatggtctga aggcgatctt
    11821 cttatatccg gtgatcggtg tcttgtttgt tggtgtagtc atgtctttat tagcaaatcc
    11881 aatggaagca gtcaaccaag gaatgatgaa tttcttggca ggattccaaa attctaatcc
    11941 attaatttta gggattatcg ttggctgtat gtcagcattt gatatgggag gtccagtcaa
    12001 taaagcggct tatgttacag ggactgcgtt gttggcacaa ggtaaccata catttatggc
    12061 aggggtttcg gcagcttgta tcgcgccccc attgattact ggatttgccg cactgttttt
    12121 tggaaaatat ttcgacacga atgatcgcaa cgctggttta gtcaacttta tcttgggctc
    12181 aacgcacatt accgaaggag caattccatt tgcggctaaa gatccgctgc gtgttctgcc
    12241 aactatgatg ctaggttcat caattgctgc tgtactgaca tatatgtttg gtgtccaagt
    12301 tcccgcacct catggaggtt ttctagtatt acctgttgtt actggtaagg ttgcatgggt
    12361 agggtcgatt ttacttggtt caatcgttgg cggagtaatt ttagggttga tccaaaaacg
    12421 tagtgtagaa aaacgtgaag cagaagcagc tgctgttgaa atgaaagaag caaccaacaa
    12481 ctaaaggtta ttctagaaag aaggatttca aatgactttg atcaaaacaa ataaagtttt
    12541 tatgaataaa aattttacga cgcaagatga ggtaatgaac ttccttgcag atcagtcaat
    12601 tgtcttgagc attagtgaag accgagatgc agtttacgaa aaattgatgg aaagagaaga
    12661 agaaagcacg actggtatga tggatggttt tgccatccct catgccaaag atgctagtat
    12721 caaggatgcc gatattgtga ttgttcggtt aaacggtaag gtcgattgga aaagtttaga
    12781 tggagaagga acggattttg taatcgctct gtttattcca gattcagagg ctggaacaac
    12841 ccatctaaaa tttttatctc aggtagccag acttttgatg cacaaagatg tgacagaagg
    12901 tctgaaacaa gcccagtctg ctgaagaaat agctgaactt ttaaaccaaa aattagaaga
    12961 aaactaaaaa ggaagtagtg aaatcatgcc tttaacagaa aaaaagaaaa cttatattcg
    13021 tcaactaagt aatgaaagtg gcatcatcag tgcgttagca atcgatcaac gtggtgcctt
    13081 gaaaaaaatg atcggtaaat ttcaaaataa atcagcaacc gatgaacaaa tcatcaattt
    13141 caaaaaaatc atttcttctg aattgactcc atatgcttca gcaatcttat tagatccaga
    13201 atatgggttg ccggcagccg aagttcgcga taaaaatgct ggtctgttgt tggcctatga
    13261 aaaaactggt tacgatgctt caactccagg acgtttaccg gatattttat ctatttggtc
    13321 tgtaaaacgt ctaaaggagc aaggggccga tgcttgtaag ttcttgttat actacgatgt
    13381 agatgaatct gatgacatca atgaccaaaa gaaggcgttt atggagcgga ttggttctga
    13441 atgtgttgct gaagatttgc catttttctt agaattggtt tcttatgatg ccaacgatcc
    13501 tgatacttct tcgaaggaat atgccttgaa aaaaccgcat aaagtaaatg aaatgatgaa
    13561 ggaattttct aagcctcgct atggagttga cgttttgaaa gtggaagttc ctgttaatat
    13621 gaactatgtg gatggttaca ctaaaaatga agttgcttat accaaagaag aagctgctgc
    13681 ttatttcaaa gaacaatccg agactaccaa cctgccattt attttcttga gcgcaggagt
    13741 aactgctgaa ttgttccaag aaaccttgaa atttgcaaaa gaggctggct ctacattcaa
    13801 tggggtactc tgtgggcgcg caacttgggc gaatggtgta gaaccattcg ttactaaagg
    13861 tgaatatgct gccaaagaat ggatgcagac acaaggacgc aagaacatcg aagagctgaa
    13921 taatgttttg aaagaaacag caacagctat taaattgtaa gaaagcttta actataacct
    13981 taagtttgta acctaatgat ttttaataat caatttgagc ttttaaataa agaattggaa
    14041 gcgtcccgac gcttcctttt tcgtttgttt ttaaggcatg ataggttcta tgttgtcgtt
    14101 caataagcgc aagaaggtca gcattttttt cttagagtat gcaaaattgc ttttctaccc
    14161 aatgatttaa aacggagagg cacccttgga tatggtagat ggtgaagtaa cgcatttctt
    14221 ctgtgatcac ggtttttttg tcttcttgta gccagatttt taaataattt tcttcgatca
    14281 gattatttaa tctcttttga aattggatat ctccgtggtt gatgacgaat aacttgtata
    14341 cgaacgtatt cgtatagaca tagtcgatca attgagcgta tagatcctcg tagctatgtg
    14401 tcgtatcgta ggaaatgatt tcatccaagt cttggaaaaa gctattttcc agtttttcat
    14461 aaagatcata gatatccgta taatgtgtat aaaatgtcga acggtgaaga tcagcagttt
    14521 cggtcagttc acggatggtg atgttttgaa tttttttatc tactaataat tttgataatg
    14581 cttctgctaa cgctttttct gtttttttga cgcggcgatc ctcttttttc attttgcagc
    14641 ctcctaatca acataatcaa ggaattgtgt cggttatccg acaatttcac cgctatctga
    14701 acatagataa ccgacacctt gtctattata atataagtat agcatatcaa tatttaaaga
    14761 ataggagaat aagtgatgga attagccttt aagcaactgg aaaagaaaga ctacaaaaaa
    14821 gccattcaat tcgcgattaa aggtatgcac tttgattggt ataccaagaa caaactcctt
    14881 ttgaatcttt atggaagata cttttggtat ctagaattga atcgcgcgac acaaatcatt
    14941 gctttatatg cggatgatga gttggcaggt gttttattag cagacatcaa aggaaaatca
    15001 aaaaagcatc attcatttag tcaaaagtta tatgttaaac tatttgattt cttacaaaat
    15061 gcatttgtga aagacagcaa aggcgggtat gacgacacca atgacgaatt gctttcagac
    15121 tatttaaaaa aacattcccc agatggagaa attgtctttt tagctgccaa ccctgattta
    15181 aagatcaagg ggatcggtag taaattatta aaagaatttg aacgccgaga gcaaggaaaa
    15241 gaagtatttc tgttcacgga tagcggctgt acgtatcaat tttatgaacg acgcggtttc
    15301 gaacggaaaa aagaaaaaga taacgtcatg aagatcctta ataaagaagt agatctccgc
    15361 tgtatgttat atagtaagat cattcagtaa tgttttttac tatcagaaag cagaagtacc
    15421 acgaaggtca atggaaaaag cgagtaattg atccatttag atgaagatag gtgtagtctc
    15481 aaagatatta gctggcaaag atatccccag ttaaaaatag atttctgtat ccaacaataa
    15541 aaatattttt gtatttatta gtatatatac tttacaaaaa tttgtatata tactaatttt
    15601 tttgtaggat caatgaaatg aggggaaaat atgccaaaat ataaagaaat agccttggct
    15661 ctaaaagaga ggatcgttga gggaaaatat caggaaggtt ctaaattacc tgagcaagaa
    15721 agtctggcta gagagtttaa aacaagccgt gttactatcc gcaaagctat acaactcctt
    15781 attgatgaag gtttgttata tacgagacgt ggatctggta cttttatacg aagcaatatc
    15841 agacaggaca atgagaatgt cactcaagta aataatgtat ttggcacttc ctcacaagaa
    15901 aatacaaatg tgacaagtaa aataatccgt tttgatgtcc ggtttccgac agattatgaa
    15961 aggcggcttt ctgcaaccta cttccgatcc ccttcctgca aatcatgggt ataaaaaata
    16021 ggcatattaa gcgggctagg aggagaaata ttcacatatc attttttcat tccgcaagct
    16081 gcgtcaagtt atttggaatt tgtagaacag atgagagagg tagaagcagc actacaaact
    16141 acttttcaat aagtaggaat tatagaaatt ttactcaaaa aatcttcatt aaaaaatctg
    16201 actaaataag ttgagaaaac agaatatact tcaatatcca gtgatttata aatatcattc
    16261 actcatgata cccagctccg tatcataagt gatgcggttt ttatcattga tcctaaaaat
    16321 cttagcaaga caaaaagtac gtaagaaagt aaagactaaa tacttcggag gaacaaatga
    16381 ataacaatga taggttattg cgattgcgtt atgcgattga tttgaaagat acggatttga
    16441 ttaaagcttt tgagctaggc ggtgtaccac tgactaaaga agaagctcag gcggtactga
    16501 ccaaagtaca ggataagaac aaagataatg cagaaaataa tgtgtatgaa aaaaccatca
    16561 acaaccaagt ttttgacgca ttcctaaatg gcctgattct cttggcgcgt ggtccgcaaa
    16621 aagataagcc gatagtagac acgccgcaaa aaagcaagga aatcaaatat atcaacaatg
    16681 tgttgctaaa aaaattaaaa atcgccttgt ctatgaccac tgatgatatt ttagaagttt
    16741 ttgctgaggc agaactctat ccatcaaaag gcgaaatcgg ggcatttttg agaaaagaag
    16801 gccaacgaaa ttttaagcct tgcggagata aatatatgcg taattttctc aaggggttag
    16861 gaatttataa tcggagaaaa gtataaagaa atttcatgag aaatcaaaac aatggttgtt
    16921 ttgatgagag ctagtttttg tatgagatta aaatattatc ttaatcgagg agctggattg
    16981 gttttatgaa tgatgaatat aagaatgatg aagataagat gctttttgaa gagatagaga
    17041 atcgatgtag gctcaatttt gagcttcggg gaaagatgtc tttaatacaa caaaaaaagt
    17101 atttagcgaa taaatcagag tttactctgg gacatgttga aaaattaatc tcggattgga
    17161 ttagtagtag aagcgaattt acaaaaataa aacagccgat taaatttgac atgaaaaaac
    17221 ttttgttaaa taagagtgag ataggtaata gagatcaata tattagagct aaagggcaag
    17281 aaataattga ttcattaggt gaaatgaggt catacaatta tttgtatgta acacatagag
    17341 ctgatggaat ggtcattact gtggggaaat cttcttctaa tgatattttt ttagatggag
    17401 atctttttta tcaactcaat acaaatcact tgtctggcac tgaaaatata attttaagga
    17461 cggaatatgg caatgaaata tttgcaaaat atgatgagat tttaaaaaat tatttggact
    17521 gggcttggat aattccggtt gagtcaggag atgctaagaa actggagaga ttattgggcg
    17581 atgaactaat taataaaaaa gtaccaattt taaattatta ttcccataga caataaaagg
    17641 ctatttttca tgataatact agttataact gtaaaaatgg aagaaaatct gggaaagaag
    17701 tttttaaata agtgtattgt tcgattttaa taaataataa gcaataccat ccgtctgtgt
    17761 tgcgcaacat cgtactacaa tgaatatttt ttcacaaaaa gaaaatcatc catcgaatga
    17821 atggaaggtt ttagtataca cgaggttaat gccccctctt ttataatcag aggagtgagg
    17881 ttttttctat gaatgtttct attttcttat tgctagtcta gtttctctta cgatgaaatt
    17941 gggattctat tccagttttt attgatttgc tgataaaata acttgaatgg tggatataaa
    18001 catgataaat aagagaagaa taacaaaaca aggttgtgaa atgattctca aatgaatata
    18061 atagaaagaa aaaggaggaa gaatcaatga aaatagcaat tgcaggcgct ggcgcaatgg
    18121 gaagtcgctt tgggttaatg cttcaccaaa gtgggaacga agtattatta atcgatggct
    18181 gggcagaaca tgtccaacaa atcaaagagc atggattaca agcaaatttt aatggtaaag
    18241 aggtagaagc aaaactacca atcgttcttc aatccgaagt agaaaaagaa gatcaagttg
    18301 atctgattat tctatttacc aaagcgatgc agctggaaaa aatgctgcag gatatccaat
    18361 cattaatcaa aaaagataca gaggtcttat gtctattaaa tggtatcggg catgaagata
    18421 ttattgagaa atttgtacca atggaaaata tctatattgg aaataccatg tggacggcag
    18481 gtcttgaagg tcctggtcag gtcaaattat ttggaagtgg ttcagtagaa ttacaaaatt
    18541 taggtgatgg aaaagaagca gctgcaaaaa aattagcaga caaattgtct gaatcaggat
    18601 taaatgctca tttttctgac aatattcact attctattta tcgcaaagca tgtgtgaacg
    18661 gaacaatgaa cggactatgc acaatcttag acgttaatat ggctgagcta ggaaaaacat
    18721 cgactgctca taaaatggtg gcgacgattg tcaatgagtt tgccaaagta gcagcagtag
    18781 agaagattga actagatgtc ccagaagtca ttgcacattg tgaatcttgt tttgacccgg
    18841 aaacaattgg gttacattat ccttcaatgt atcaagactt gattaagaac catcgattaa
    18901 cagaaatcga ttatatcaat ggcgcaattt ctagaaaagg gaagaaatat ggtgttgcga
    18961 cgccttattg tgatttcttg actgagcttg tccatgcaaa agaagatagt ttgaacgtaa
    19021 aataaaggag agattgaatg gaaacttcac aaaaaatatc accgaaaatt tttctgaata
    19081 aagtcctcgc tggtacagca acaggaatca ttattggatt gattcctaat gcagtattag
    19141 gtgccatttt aaaatatttt agtgcgtatc cctttgcggt aacgatcgca cagttagcag
    19201 ttatttttca actagcaaca cctttgatca ttggtggttt gattgcctta caatttggat
    19261 ttgatccgat gagaatgatg gttgtagccg gagcttcttt tgttggttct ggtgtagtca
    19321 aattcacacc tgatttgggt gaaacgggtg tttacgttgg tgctggtaca ggcgatatta
    19381 ttaatacgat gctgacagca tcgattgctg tcgggctgat tttactgatt ggaaatagtt
    19441 ttgggtcagt gtcgattgtg ttaacaccga ttgttgtagg tgtaggtgca ggtttacttg
    19501 gtttttattt attccctttt gtaacagcaa ttacttctgc aattggatca ttgattaata
    19561 acttcactac cttacaaccg ctgttaatgt ctatcttgat tggttgttcc tttgcctttt
    19621 tgattatctc tcctatttca acagtagcaa tcggaatggc aatccaacta gatggtgttt
    19681 ctgctggtgc ggctgcgatg ggcgtagctg caaccacttt tgttttagta gtgaactctt
    19741 ggaaggttaa taagtcaggt gtgacaattg cgattgcttt aggcgctatg aagatgatga
    19801 tgcctaatct atttagaaaa cctgtgattc ttattccttg tttagttagt gcagtgatta
    19861 cagctattcc tgtcgctcta ttttctattt caggtacgcc tgcttctgct ggtttcggcg
    19921 tggtcggact tgttggtccg ttggcttctt tagatgcagg gctaaatggc tttcttgttg
    19981 ttctttgttg gttagtcatc cctgtttttg cggcactaat tactcaattt ttatgcgaga
    20041 aagtatttaa actgtatgat cgagaagaag tcttcaaatt tttaggctag tagattttac
    20101 taagaaattt tcaatcaatg gatgcgtaag tgagatgtca acaacatctg acttacgttt
    20161 ttttgccttt ttgagtagga gattgatgct gacaactgaa ctcttcagtg ccattcgatt
    20221 ttttaaaatg tagaagaaac tcacctttat gttattgatt gttcaaaagg caactagaat
    20281 aaaaatatgt gttaaaatga gaaaaaattt gattgttgga tgtatcgaat aatcactaaa
    20341 aagtaggaga agaaaatgat ttttttgcaa aaatatggtt tttatttttt attattaggt
    20401 gtgattagtg atttatcgac accgtatatc cttggccttt tttatcccaa actgaatcaa
    20461 atgactaccg taattagtgt gtttggtgac gtagatagtc cagtcagacg tgctttcttg
    20521 gtctggtcag ttgtatctgg actttttttt gttctttcgt tgcctgcgtt gtatcaccta
    20581 tttattggaa catcaaaaac acttgcaata cttgctgttg caacagttgg tttatatggg
    20641 ataggtgatt gtatttttac tgggttattc agtatcaata cgaatgaaag ctcttggaat
    20701 ctatcgactt ggatccataa tataggttct gggctaggct atgcaggctt tctacttttc
    20761 ccattgttac ttgttctgct ttatcgtcaa agtggtcaag gtacgcttag ccatttttat
    20821 cttgttttaa cagttattag tttactaatt gcaggtcttt acggattagc acggattccg
    20881 tcgattagtc agtttgcctt ctttaagcaa ttgggcttct ttcaacgctt aagttttttc
    20941 tttaactatt tgccgatgat ttgtttaagt ttttttcaac tacgaaagta gaaaaagcag
    21001 tgtgaagctg attaagtaaa agaatttctc tatgggtaac cattcgtgtg gatgtgttcg
    21061 actaagaata gagatgagga gagcgaaaag caagttagtc ttaacttgtt atgcgttgtg
    21121 ctaaatttca ggtagtcatt ttttgaatga ataagaagaa aacttgaaag agggtgactt
    21181 catgttagat gtggagcaga tggaaataga cctttataat attattttaa atcgaaatac
    21241 aagagaatgg gaaagaacac aatttgaaga ggcaaaagaa gacctagcaa aaatgtcgat
    21301 aaagcaggtg atagaaaaac tagaggtcaa cttgcgtccg ctagcactac gagataattt
    21361 gacaccagat gcgatggatt tttatttaag aatcacaggg gagtcagcga gtgcaaaggc
    21421 ttatgattat gccaaacata agataacttc acccttatac caagaacggg cgatttttgc
    21481 cggtggctgt ttctggtgta tggttgagcc ttttgaaaca aaaccaggtg taatctcagt
    21541 gttatctggt tatacaggtg gagagaccaa aaaaccaaat tacgatcaag tcagtggtgg
    21601 atatactggg catgttgaag ctgttgagat catatttgat acacgtcaaa taacctatga
    21661 agagctgttg gagatttatt ggcaaatttc agaccctaca gatgcctttg gtcaatttca
    21721 agatcggggg aaacaatatc gttcaattct atttgttgtt aatgaaaagc aaaggcaatt
    21781 ggctgaaaaa tcaagagaac aactgattca atctaaccga tttagccgtc ctatcgttac
    21841 agagattcgc caagcaaaga ctttttggcc agctgaaaat catcatcaac aattttatca
    21901 gaagcaacct aaaagatata aaaagatcaa gcattcgcat cagcttcatt tattttttca
    21961 gcgacataga tagaggaagg ttgtggcgca aagcgttcag ctccgacaaa ataatgagca
    22021 actgacaaaa atagcttttc atatttttgg acagttatga attatttcga ggagttgctt
    22081 tgagaacacc gaggaaaagg ttgtggcgca aagcattcag cttcaagtcg caaggaaaca
    22141 ggaggtatga caagtctttt gtcacaactc ctgttttttt actagccttt atttccatgt
    22201 gaaagagaat tcgaattgta gaatcttgat ttttctatgg aattttgtag agaatcatct
    22261 tgataattaa tagacaccta atcgaattcc acattaatga tgaactaaat tccgtataaa
    22321 agcgcaaaca ataactata
//
LOCUS       DACEKG010000045        22026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEKG010000045 DACEKG010000000
VERSION     DACEKG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.046"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(61..1371)
                     /locus_tag="IW664_001667"
     CDS             complement(61..1371)
                     /locus_tag="IW664_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0849029.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IW664_001668"
     CDS             complement(1383..2801)
                     /locus_tag="IW664_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0849030.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IW664_001669"
     CDS             complement(3041..4609)
                     /locus_tag="IW664_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0849031.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IW664_001670"
     CDS             complement(4613..4894)
                     /locus_tag="IW664_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0849032.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IW664_001671"
     CDS             complement(4891..5814)
                     /locus_tag="IW664_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0849033.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IW664_001672"
     CDS             complement(5811..6689)
                     /locus_tag="IW664_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0849034.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IW664_001673"
     CDS             complement(6676..8013)
                     /locus_tag="IW664_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0849035.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IW664_001674"
     CDS             complement(8016..9119)
                     /locus_tag="IW664_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0849036.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IW664_001675"
     CDS             complement(9193..10701)
                     /locus_tag="IW664_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0849037.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IW664_001676"
     CDS             complement(10667..11359)
                     /locus_tag="IW664_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0849038.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IW664_001677"
     CDS             complement(11381..12355)
                     /locus_tag="IW664_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0849039.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IW664_001678"
     CDS             12655..13527
                     /locus_tag="IW664_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0849040.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IW664_001679"
     CDS             complement(14109..16307)
                     /locus_tag="IW664_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0849041.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IW664_001680"
     CDS             16539..17531
                     /locus_tag="IW664_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0849042.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IW664_001681"
     CDS             17591..19012
                     /locus_tag="IW664_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0849043.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IW664_001682"
     CDS             19005..20504
                     /locus_tag="IW664_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0849044.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IW664_001683"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IW664_001683"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0849045.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DACEKG010000046        21600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEKG010000046 DACEKG010000000
VERSION     DACEKG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.047"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(350..1846)
                     /gene="lysS"
                     /locus_tag="IW664_001684"
     CDS             complement(350..1846)
                     /gene="lysS"
                     /locus_tag="IW664_001684"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0849046.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1957..2961)
                     /gene="dusB"
                     /locus_tag="IW664_001685"
     CDS             complement(1957..2961)
                     /gene="dusB"
                     /locus_tag="IW664_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0849047.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2962..3849)
                     /gene="hslO"
                     /locus_tag="IW664_001686"
     CDS             complement(2962..3849)
                     /gene="hslO"
                     /locus_tag="IW664_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0849048.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4066..6177)
                     /gene="hflB"
                     /locus_tag="IW664_001687"
     CDS             complement(4066..6177)
                     /gene="hflB"
                     /locus_tag="IW664_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0849049.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6267..6812)
                     /gene="hpt"
                     /locus_tag="IW664_001688"
     CDS             complement(6267..6812)
                     /gene="hpt"
                     /locus_tag="IW664_001688"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0849050.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6812..8257)
                     /gene="tilS"
                     /locus_tag="IW664_001689"
     CDS             complement(6812..8257)
                     /gene="tilS"
                     /locus_tag="IW664_001689"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0849051.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8377..8847)
                     /locus_tag="IW664_001690"
     CDS             complement(8377..8847)
                     /locus_tag="IW664_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0849052.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8893..9318)
                     /locus_tag="IW664_001691"
     CDS             complement(8893..9318)
                     /locus_tag="IW664_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0849053.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9385..9654)
                     /locus_tag="IW664_001692"
     CDS             complement(9385..9654)
                     /locus_tag="IW664_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0849054.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9665..11260)
                     /locus_tag="IW664_001693"
     CDS             complement(9665..11260)
                     /locus_tag="IW664_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0849055.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11275..14796)
                     /gene="mfd"
                     /locus_tag="IW664_001694"
     CDS             complement(11275..14796)
                     /gene="mfd"
                     /locus_tag="IW664_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0849056.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14813..15373)
                     /locus_tag="IW664_001695"
     CDS             complement(14813..15373)
                     /locus_tag="IW664_001695"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0849057.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15726..16700
                     /locus_tag="IW664_001696"
     CDS             15726..16700
                     /locus_tag="IW664_001696"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0849058.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16870..18270
                     /locus_tag="IW664_001697"
     CDS             16870..18270
                     /locus_tag="IW664_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0849059.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18348..19070)
                     /locus_tag="IW664_001698"
     CDS             complement(18348..19070)
                     /locus_tag="IW664_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0849060.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19149..19655)
                     /locus_tag="IW664_001699"
     CDS             complement(19149..19655)
                     /locus_tag="IW664_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0849061.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19778..21577
                     /locus_tag="IW664_001700"
     CDS             19778..21577
                     /locus_tag="IW664_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0849062.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6000 a         4717 c         3877 g         7006 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
       61 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      121 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      181 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      241 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      301 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      361 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      421 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      481 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      541 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      601 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      661 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      721 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      781 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
      841 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
      901 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
      961 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1021 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1081 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1141 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1201 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1261 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1321 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1381 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1441 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1501 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1561 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1621 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1681 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1741 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1801 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     1861 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     1921 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     1981 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2041 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2101 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2161 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttctcctgt
     2221 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2281 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2341 atccccgttc cccataaatg ggatcgtcaa atgttttttc acttctttca atacttccca
     2401 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2461 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2521 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2581 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2641 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2701 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2761 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     2821 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     2881 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     2941 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3001 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3061 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3121 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3181 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3241 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3301 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3361 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3421 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3481 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3541 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3601 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3661 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3721 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3781 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     3841 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     3901 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     3961 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4021 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4081 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4141 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4201 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4261 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4321 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4381 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4441 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4501 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4561 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4621 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4681 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4741 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4801 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     4861 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     4921 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     4981 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5041 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5101 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5161 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5221 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5281 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5341 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5401 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5461 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5521 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5581 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5641 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5701 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5761 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     5821 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     5881 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     5941 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6001 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6061 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6121 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6181 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6241 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6301 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6361 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6421 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6481 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6541 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6601 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6661 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6721 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6781 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     6841 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     6901 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     6961 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7021 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7081 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7141 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7201 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7261 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7321 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7381 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7441 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7501 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7561 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7621 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7681 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7741 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7801 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     7861 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     7921 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     7981 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8041 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8101 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8161 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8221 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8281 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8341 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8401 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8461 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8521 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8581 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8641 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8701 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8761 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     8821 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     8881 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     8941 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9001 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9061 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9121 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9181 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9241 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9301 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9361 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9421 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9481 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9541 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9601 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9661 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9721 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9781 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
     9841 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
     9901 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
     9961 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10021 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10081 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10141 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10201 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10261 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10321 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10381 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10441 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10501 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10561 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10621 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10681 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10741 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10801 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    10861 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    10921 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    10981 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11041 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11101 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11161 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11221 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11281 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11341 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11401 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11461 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11521 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11581 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11641 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11701 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11761 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    11821 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    11881 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    11941 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12001 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12061 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12121 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12181 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12241 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12301 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12361 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12421 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12481 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12541 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12601 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12661 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12721 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12781 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    12841 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    12901 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    12961 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13021 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13081 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13141 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13201 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13261 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13321 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13381 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13441 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13501 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13561 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13621 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13681 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13741 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13801 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    13861 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    13921 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    13981 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14041 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14101 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14161 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14221 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14281 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14341 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14401 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14461 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14521 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14581 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14641 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14701 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14761 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    14821 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    14881 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    14941 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15001 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15061 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15121 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15181 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15241 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15301 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15361 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15421 ctaattagcg gtccagtcca tcgagctttt ttctttttta tacttgtcaa tgcatagtat
    15481 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa ataaattaaa
    15541 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15601 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15661 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15721 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15781 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    15841 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    15901 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    15961 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16021 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16081 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16141 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16201 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16261 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16321 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16381 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16441 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16501 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16561 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16621 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16681 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16741 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16801 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    16861 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    16921 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    16981 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17041 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17101 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17161 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17221 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17281 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17341 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17401 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17461 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17521 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17581 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17641 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17701 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17761 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    17821 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    17881 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    17941 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18001 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18061 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18121 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18181 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18241 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18301 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18361 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18421 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18481 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18541 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18601 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18661 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18721 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18781 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    18841 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    18901 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    18961 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19021 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19081 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19141 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19201 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19261 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19321 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19381 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19441 agatggtcga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19501 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19561 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19621 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19681 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19741 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19801 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    19861 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    19921 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    19981 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20041 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20101 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20161 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20221 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20281 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20341 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20401 agaaacactt ttcgcagcat gggaagaagc ctgggaagaa aaggcacccc tcttcacgga
    20461 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20521 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20581 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20641 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20701 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20761 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    20821 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    20881 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    20941 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21001 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21061 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21121 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21181 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21241 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21301 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21361 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21421 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21481 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21541 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
//
LOCUS       DACEKG010000047        21317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEKG010000047 DACEKG010000000
VERSION     DACEKG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.048"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW664_001701"
     CDS             <1..111
                     /locus_tag="IW664_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0849063.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(569..844)
                     /locus_tag="IW664_001702"
     CDS             complement(569..844)
                     /locus_tag="IW664_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849064.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1298..1504
                     /locus_tag="IW664_001703"
     CDS             1298..1504
                     /locus_tag="IW664_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849065.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1700..1867
                     /locus_tag="IW664_001704"
     CDS             1700..1867
                     /locus_tag="IW664_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0849066.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1893..2237
                     /locus_tag="IW664_001705"
     CDS             1893..2237
                     /locus_tag="IW664_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0849067.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2242..2523
                     /locus_tag="IW664_001706"
     CDS             2242..2523
                     /locus_tag="IW664_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849068.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2626..2940
                     /locus_tag="IW664_001707"
     CDS             2626..2940
                     /locus_tag="IW664_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0849069.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2918..4612
                     /locus_tag="IW664_001708"
     CDS             2918..4612
                     /locus_tag="IW664_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0849070.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4632..5810
                     /locus_tag="IW664_001709"
     CDS             4632..5810
                     /locus_tag="IW664_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0849071.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5773..6459
                     /locus_tag="IW664_001710"
     CDS             5773..6459
                     /locus_tag="IW664_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0849072.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6459..7619
                     /locus_tag="IW664_001711"
     CDS             6459..7619
                     /locus_tag="IW664_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0849073.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7629..8504
                     /locus_tag="IW664_001712"
     CDS             7629..8504
                     /locus_tag="IW664_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849074.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8501..8812
                     /locus_tag="IW664_001713"
     CDS             8501..8812
                     /locus_tag="IW664_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849075.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8802..9155
                     /locus_tag="IW664_001714"
     CDS             8802..9155
                     /locus_tag="IW664_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0849076.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9145..9546
                     /locus_tag="IW664_001715"
     CDS             9145..9546
                     /locus_tag="IW664_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849077.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9539..9943
                     /locus_tag="IW664_001716"
     CDS             9539..9943
                     /locus_tag="IW664_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849078.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9955..10563
                     /locus_tag="IW664_001717"
     CDS             9955..10563
                     /locus_tag="IW664_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0849079.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10583..10945
                     /locus_tag="IW664_001718"
     CDS             10583..10945
                     /locus_tag="IW664_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849080.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10948..11130
                     /locus_tag="IW664_001719"
     CDS             10948..11130
                     /locus_tag="IW664_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849081.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11147..14578
                     /locus_tag="IW664_001720"
     CDS             11147..14578
                     /locus_tag="IW664_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0849082.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14629..15366
                     /locus_tag="IW664_001721"
     CDS             14629..15366
                     /locus_tag="IW664_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0849083.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15376..17667
                     /locus_tag="IW664_001722"
     CDS             15376..17667
                     /locus_tag="IW664_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849084.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17691..19817
                     /locus_tag="IW664_001723"
     CDS             17691..19817
                     /locus_tag="IW664_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849085.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19980..20426
                     /locus_tag="IW664_001724"
     CDS             19980..20426
                     /locus_tag="IW664_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849086.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20428..20565
                     /locus_tag="IW664_001725"
     CDS             20428..20565
                     /locus_tag="IW664_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0849087.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            20603..20896
                     /locus_tag="IW664_001726"
     CDS             20603..20896
                     /locus_tag="IW664_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849088.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            20893..21117
                     /locus_tag="IW664_001727"
     CDS             20893..21117
                     /locus_tag="IW664_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0849089.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            21114..>21317
                     /locus_tag="IW664_001728"
     CDS             21114..>21317
                     /locus_tag="IW664_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0849090.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMK"
BASE COUNT         7510 a         3206 c         4452 g         6149 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcata taaacacgga aaaataattg cttataaata atttggcggt
      121 tttttggcgg aatgatggcg gtttttagac atttaccagt gttattatgg tagtgtcgaa
      181 agattaggaa acaggatcga caaaataaac gaagggaggc aatctccctc atcgttttaa
      241 acttcttctt agatagacag caactaatta tgaagaaaag gatgtgaatt caactcctcc
      301 taaattcttc atcggttgct gtttattttg attagacagc agcactcaca aaactaaaca
      361 tgttcttgtc gtctttcaac tgctgctgtc tattaattta tgtattggag ggaagaaaaa
      421 tgaaattatc agtagaaggc actccagaag aaatacaaga attgctccaa gctattggta
      481 gtagcaagga gcaaaaggta agtgtttcta attcagatat tgacggtatt tgcaattgtt
      541 taaacagaga aaaaatatct aatccttact aagagaaaat atgttcgtta atgaatttcc
      601 aattttcctc tgtagctcta ccttgataat ttttttgcag ttcacagatg aataaggaat
      661 cgttattatc aagtactgta tttaattttt cacacatttg atttggattc aaattagttt
      721 taactaacca aaaagatttt tgtagcttta tatatgcccc aaattctttt attatttcaa
      781 atactttatc atacttttgc cctgggttat taagatcata actaatcatg tagggtttat
      841 acatgttgtc ttgcacatcc ttgttttata tttcagcgga ccactcgctg ataaataaaa
      901 ttatacgctt agtatttatt ttcacaatat taatttatcg taataacttt tgagtaaata
      961 gaacaaaaaa cctgcactag tttccgctag tacaggcagt gactatatcg gttgataatc
     1021 tagcatatat taaaaataat tgcaagaaaa gaaatttata gtggtttata ccaaattatc
     1081 aaagcaatgt tattttgttg ctgtctattg ttttttaatt attcacacga taactaaagg
     1141 tgggtgaaga gaaatgattc cattaataat ttcaattttt gcgctctgtc ttaatgtcta
     1201 tatgattgga tttaaaaatg ggcaaaataa aaaatagtag cagccaagaa taattttata
     1261 gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa
     1321 ccatgcaagg ttcgattcct tgccagcgac attaaatgcc tatgacggtt acgactaccg
     1381 aaaaaagatc gttaagaagc tatacggtgc tacgtacggc aatgtagtaa gtgtgctatc
     1441 tgtacaccac caagcttcgg tcactgtggc ggaagtagaa gacgcagcgg taaatggcga
     1501 gtagcctcgt gagagcctgg taagttctcg tgagtggtgc aatcccactc cagcgacttt
     1561 aagcaaccga ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg
     1621 tgcatcgtgc aagttactat tacatattag atcactcttt gagtggtctt tttatttttg
     1681 cacaaaggag gaaacaacaa tgtatagacc acaatactta gaacagaagt atgaagtaat
     1741 cactgttcat aaaggtaatg gcgaaaaagt atatgagtat agaagaccaa taaagagcga
     1801 tacatataaa cgaaaggaaa acaatgaagt tattccattg tatggcaaaa gaatagctaa
     1861 gcattaaata agattgcgaa aggagacgga acatgaccga ggaattctat agatggctat
     1921 tacagttgac aagagaagat cgtttggtta agttctatca gtctcctaaa tggcgcaggc
     1981 ttagagagaa agcgatgaaa cgagatcact atgaatgcca agagtgtaga agactaggta
     2041 agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga cctgacttag
     2101 ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa gttcatggca
     2161 gatatcttac agcattagat aaacaagaga agaagataga aagctttgct aacttcgatg
     2221 caagtgaaag gtggtaagtg catgatcatc aatgataatg gcagagagta tgatacagaa
     2281 aagattgaag agtattcatc ttatactcag ggattaatta aacgtttgat atacgttcgc
     2341 tatgtaggta ttagggatct gttatcagat aactgttgta gtaaatacaa agtgaatcaa
     2401 gtaagagaag cgttgaataa agataataac gtcgaaagaa taaaaaatgt ttttggatat
     2461 agtattgaag agattaatta ttacattgac ttcgctgaag ctttcattcc gatggtgaga
     2521 taaccccccc ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga gggggctgtc
     2581 aggaaaagag attttttcga actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa
     2641 ttgtctcaaa atcgctacag agaaaaatta cgccgctctt taataagcca attggaaagt
     2701 cagaaaacaa atattgagcc attcttagat aatgttgatc gttatatcag tttatgggaa
     2761 acggcgatat cactggaaga agatatatcc gagaacggca ttagactgga gaatggtaaa
     2821 aagaatgaat cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt gatgttggat
     2881 aaacttgcca ttactcctga attggtaggt gaagcaaatg aatcaattcc tgagttataa
     2941 gcatattgaa aattggttca aagctataga agaaggcact atcaaggtat gcaaagagca
     3001 attattgcta aaaaattatc tagaagaaag agtctttact agagaagata tttacttcga
     3061 taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat tcgaattaat
     3121 tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa aagataaaac
     3181 actagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa ctggttttgc
     3241 ttcttggaac aacttctttc tactaaccgc taaacacggt attaaaaatt atgatattga
     3301 tatctatgcc aataatgaaa gccaagcaaa gactagtttt gatgatgtat ttaaagtaat
     3361 taaagatcat cctgatttag ataaaaaagt atttaaagct acgaaggaag ttattcaaaa
     3421 tatcgctaca aacagcaaac ttcgttataa cacggcaaat gctagaacaa aagatgggaa
     3481 gcgaccaggt gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt caatgataaa
     3541 tgtggctact tctggtggtg gtaaaattcg agattataga gaattttatg atacaactaa
     3601 tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa tgattctttc
     3661 tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc catttatttg
     3721 tcgcttggat aacgataatg aagttgatga tcccgacatg tgggaaaaag cttgtccaac
     3781 tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc aaatgcaacg
     3841 taatgctggt ttaagactta cgttcatgac caaacgaatg aacagaccta tggaagatac
     3901 acgatttgct gttgcttcat atgatgatgt tctgcatacg aaagaaaaag aatttcctga
     3961 aaaaatggat gaagtgatag gaacagtcga ttttgctgat agacgagatt ttgccagcgt
     4021 tgggttgcta ggaaaatacg ataaagatgt ttattttaca caacatactt ttatccacga
     4081 atcagccctt cgattacaaa acatcaaacg agaggttata gatatttcta tagatcaagg
     4141 aaaatcacag atcgttcatg gaaaaaatat agaagctgat tatattgtag gttggtttct
     4201 tgaaatgagt aataaatatt atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat
     4261 attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt
     4321 tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt
     4381 tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa
     4441 tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc
     4501 attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac
     4561 aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag
     4621 gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg
     4681 ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga
     4741 ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg
     4801 aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga
     4861 aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag
     4921 tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg
     4981 tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc
     5041 ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta
     5101 ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg
     5161 tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag
     5221 acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg
     5281 aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca
     5341 gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga
     5401 tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat
     5461 cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg
     5521 atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg
     5581 tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag
     5641 gcattagaaa tggagtattt acaatcaacg aaggaagaga acgcgttgga aatgatcgct
     5701 ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag
     5761 gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta
     5821 ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac
     5881 aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt
     5941 aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat
     6001 aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca
     6061 atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc
     6121 acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt
     6181 gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa
     6241 gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc
     6301 gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt
     6361 gaagaaattt tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca
     6421 gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag
     6481 gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt
     6541 ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa
     6601 cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac
     6661 aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat
     6721 tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat
     6781 tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag
     6841 tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct
     6901 ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa
     6961 tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat
     7021 tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa
     7081 tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta
     7141 ttacagcttt agatgctgta actatgggcg gtttgatggc gcgactatca aaattcaata
     7201 tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctaattg
     7261 tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg
     7321 gagaatacgt acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag
     7381 aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa
     7441 aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac
     7501 aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt
     7561 tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg
     7621 tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg
     7681 aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca
     7741 gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt
     7801 acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga
     7861 aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg
     7921 tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt
     7981 gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa
     8041 atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa
     8101 acactaattc aggaggcgca cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa
     8161 aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac
     8221 aaatctggac agccgagatg tgggcacagc tttggaacat gatgtatttc aatattggtc
     8281 ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag
     8341 aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta tttttcaaag
     8401 ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat
     8461 gctcttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac
     8521 tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga
     8581 gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac
     8641 actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga
     8701 ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat
     8761 tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac
     8821 tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg
     8881 aactaggaaa aaagattggt gtagaagata cagaaatcac ttctttaaaa tttagaaatc
     8941 tttccatgag agatagtgat ataacagcta tggatgcgat gggatcgaaa ttaactaaga
     9001 aagtaaagac tccatttcat ccaatcgcca agaaatttaa taaagatcaa tattttatcg
     9061 taatcgatag tatgcgttac aacgttatct atgccgatta cgataatttt tatatctatt
     9121 tttatcttga aagtgtgggt gaatatggtg attgataatt ctaaagaaaa agaacgttta
     9181 aataagcaaa tttctgctat caaaacttcc ttagaagagc attttggcct caaactcttt
     9241 caagactccg taggcgagga tgagctacct gatgatttta attacttcat tctcgaaaca
     9301 ggagaaatag aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct atatctaact
     9361 ttctattcag aaaatagaga agatttaaca ggagactcac tagatattat ttcattgatt
     9421 caaaatcgtt cgattcgttt tcagagaatg gatcccaatc atttaaaact agagaaccaa
     9481 gatcgctata tcgatcaatt ggtatttacg tttagacgat tattgaagag tgattgtcat
     9541 ggctaaaaat agttgggagc taaaaataaa tggacatgat gaacttcttg tgcggatgga
     9601 acgctattca agcgagagcg aacgactgat taacgaagca ttgaaatcaa aaggttcggc
     9661 tattgcagtg gataggatta cagaaaaaat tcctgtttct gaagcagatt taagaagagg
     9721 gcaccaacac gcaaaaaata gtcgtccact taagactcaa tacattaatt tgggtttcat
     9781 cattagacct acaagaaaat ttgagtattt aaaatatcct gatttgggga taggtacttc
     9841 taaaagaaat cagccggacg aatttatgag aagaggatta ggtcttgcac ttgatccaat
     9901 tacagaactt ctgattcgtc aattcgataa attaaataaa tagggggaac aacaatggct
     9961 aaaacaaaaa ctgtagtaac aacgttcgat aacgtaagta tcaaacgaat tgcttttaat
    10021 tttaagaacg caggaaatgc aatcgcaacg aattgtaacg gacaattaga tggcgaaaca
    10081 gaaatgcaaa cggtggttaa aaaatgtgga gcgacagaag taaaatcaaa atctaaacca
    10141 atcaatatga cggtaacaat tactgcacat gtaccgatgg aagtttatcg acgtttcaat
    10201 gggttgaaac aagatggacg tattaaacca ggcatttact cttacggtcc tgattccgta
    10261 ggcgaagatt tctcacttgc tgcagagatc gtggatgact tcgaagaaaa tagaaagtta
    10321 gttggtatgt tagcatgcac ttcgaataca ggattaacat tctctattga aaatggtgcg
    10381 gatgaagtag ctgcgttaga actagaaaca aaagttatgc aagatgaatt tggtaaattt
    10441 tatcatgaag caattgttgc agaacttgaa gaagacttaa cagatcaatg gatgacgaat
    10501 ctatctgctg atgtgattaa aaaaacttca gttgtgacaa ctacggccac tcaatcacag
    10561 taaaaaaaac ggaggtagcg aaatgaacga agattactca aaaattgaac taaacgatgg
    10621 aacaattttg aatttagaac ctaaactgaa tatcaagaaa ttattgatga tcaatagaga
    10681 ttttaacaca gacgagtttg caaaaatgac tgtgggaaaa ggatccatgg atatttctgt
    10741 tattcaaggt gcaaaggctg tgtatattgc ttaccgccaa gcgaacatga ctgattatat
    10801 ttcattcgat gaatttatcg ataaatggga ttttgatatg gctactgcca gctatattta
    10861 tcaattgatg atgttcaaac aagcacgcga tgcttatcaa aaagaatttg aaaaagcaaa
    10921 taaggaaaaa aagcttcaaa agtaaaaatt ccaaagctct tagttgaaac gtgggtcgat
    10981 gtctattcga tgttgaccga cgttttttct atgccttcag atttggtttt aagcgatatc
    11041 tgtttagatg acattttgca aatggcttac aacaagagtg cttatgaagg atggaaaaac
    11101 tatgcaataa accaatccca gaaaaactaa agaaaggagg taaaaaatgg ctaaaaagag
    11161 aacagaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga
    11221 aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag ctcaattaca
    11281 acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag aaaagcagta
    11341 tgaattacaa agtcaaaaag ttgaagtaac tagccaacgt ttagccaatg cgaaaaaata
    11401 ttatggagaa aattccaccg aagttaagaa acttgaaaga gaactgatta atcaacaaac
    11461 agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatgcactag ctcaagcaaa
    11521 aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagcgaac aaaaaaatgt
    11581 tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagcaa ctgctggtca
    11641 atcagcttct gaagccgaga aattagcgaa agcccaagaa tatgtttctc aacaatctga
    11701 aaatgcggag aaaacgatag atatcctaag acgacagtta gaagctacac agtctgaatt
    11761 tggcgctaca tccacagaag caatgcagat ggaagcgaag cttaatgatg ctgaacgtga
    11821 atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg acgatatcgg
    11881 aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg acattggcga
    11941 taagcttaca gaattaggga aacaagcagt ggactctgct aacagtgtag gtagttccca
    12001 gagtaagata caagctaatt ttggtttgtc taaacaagag gctgaagaat taacgaatgt
    12061 agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca cagatgcatt
    12121 gattttggta aagcgtaatt taggagattt aaataatcaa gatttacaaa atatcacgga
    12181 acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt tacgtggtgt
    12241 aaatggctta atggtcaatt tcggcttgag tgctcaagat gcaatggatt taatggtttc
    12301 gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg cagaatatag
    12361 ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg
    12421 tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg aaatgggaat
    12481 atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa gtactagaaa
    12541 agcttttgaa gagtggaaaa atggcggagg aacacaaaaa gacgttatta attccatgat
    12601 tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt cgacaatttg
    12661 gtctgcgctt ggcgaagata atgcgatgaa agttgtccaa tctttaactg atgttaacca
    12721 tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta ctactccgtt
    12781 gcaagagttg aacgggaaaa tagctgaatt aaaggattca ttagctccta taggcaacac
    12841 aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg atgcgtttaa
    12901 taatcttcca caaccagtac aggatttcat tgttgctata ggtggtctta cagcagcatt
    12961 tggcatactg ttaccggcaa tactagccgt gtcatattta tttggtccga tgatgcttat
    13021 aattggagga attatagctg ttatagcagg cgttattgtg gcaattaaga actggggtgc
    13081 aattactgac tggtttagtg gtttatggaa aaaatttact gattggttgg gtgatacttg
    13141 ggaaagtata aaagacggag cctcatcagt ttgggatggg gttaaagaaa cctggtctgg
    13201 atttgtagat tgggttcaag atatttggca aggagtttct gattggtttg gagagctatg
    13261 gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaaa cttggcaaac
    13321 attcgttgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt cgattatttg
    13381 ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat tgattcaagc
    13441 tggtattaat attatcgtgg gtatttttga tgtagctgga cagttattgg gcgcagcttg
    13501 gcaagctgtt tggacaccta tttctgattt ccttaaaaat acttgggata ctatgacaca
    13561 atggataagt atcgcttgga atgggattgt aactacattc catactatat ttgatccagt
    13621 agtggcatgg tggaatggta tatggacagc tattagtact acggcttcaa atatttggaa
    13681 ttcaattagt gcaacagctt ctagtatttg gaacagtatc aagaatacaa tcactagctt
    13741 ggtacaagca gctgctacag taattcaaaa tatttggtca actgtatcta gttggttagg
    13801 tggaatttgg aattcaatca gctctacagc atcaaatatc tggaatagtg tgactagtag
    13861 tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga atagtatatc
    13921 ttcgtggatt agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg
    13981 aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg
    14041 gtccaatcta acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa
    14101 catatgggaa ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat
    14161 cagtggcatt tttgataatt tgaatatttc tataccacat attccgttac cggaatttat
    14221 catggaaggc agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca
    14281 tgctaaagga ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga
    14341 agcaggacct gaagcagttt tacctttgaa aagatctgtt ttgcaagaaa ttggtgatcg
    14401 tatcttgagt agcacatcag tttcatctag ggcacaaacg attcaacctg tgaataacta
    14461 cgaattcaat ttcacaattg atggtaacgc agatgaggtt actatgaagc aaacaactca
    14521 acaaatcatt gatagcatta caaaaattca aaatgataat gcttcggcat ggcgttaaac
    14581 aggagagtat ttctcctgtt tttttagtat taaaaaggat gtgaaaaaat gactgattgt
    14641 atacattcta taatcgatgg atttcctgat tatttgcata aattggcttt agctgaaaga
    14701 ccaaccatac cttctccaaa aagacagaga gttgaaactt ctgttttagg taggttaggt
    14761 ggcttagtac aagattactc gtttgaagat atgtcgttta cattgcacta taactattta
    14821 gaggatgtgg aagaccatca agcgttcaag caatcgtttt atatcatgcg tcattggtta
    14881 aactatgcaa agaaattaga attctctgac gatcccaacg tctattacgt tatccagact
    14941 atcgatattg gggatgcaga aaacgatatt gttgaatggg gagagttcga tgtaaatatt
    15001 actgcgaaac cattcgcaag agttcaagaa gatgtaccta taaccgtaga taaaccacag
    15061 tcatttaact tgctgaataa tagtttagaa gaaagttttc caaagattat catcactcct
    15121 tcagctactt catgccagtt catcttaaat gattatgtgt ttagtttcga aggcttagta
    15181 gcaggaactg atgtagtcat tgatagtgat ttgatgcttt gctacgaaga gcaatcggac
    15241 ggagatattt tagatcggtc caacaaaatg aagaccatgc aatatccgac attgcaagtg
    15301 gatatcaatc attttaattg cactggtttg agcaaaatac aaatttatcg taacgggtta
    15361 aggtaggtga aatagatgat cgataattta ataactattt acgataaaaa tgacgcgaat
    15421 aatttagctg aacatttata tgatacgcaa ggtttaggcg ctttgtcaga ctggttaaca
    15481 gctactgtta gcaataaact aaacggagcc gagatatttc agggtactta ttcaataaac
    15541 ggaactaatg cagatttgat tgtagaagga cgtattattc agtgttatgt agatgaaaat
    15601 cgagcaaaac agcgtctacg gatttattat gcaaaaactt ctgtaatagg caatacgata
    15661 gaagtaaaag ctgaacctat tttcaatgat ataagaaaat cggtgttgaa taaatatgac
    15721 agcggaacag aaaagatcac tgctactcag gcatggcaaa acgcaaaagt tttagcgaaa
    15781 ccagctatcc cttcgcagtt ttctttctcg tcattagtag atacgcttgc taatgtgaag
    15841 atagaaaagg cgaatttttt agaattcttt ggtggaaaag agggatctat tctagatcga
    15901 tttcatggtg agtttctaaa agataataac acattacgtc atgaaaaaag tctaggtacg
    15961 gatcataaaa tcaaagcgat ttatactaaa aacttaactg gtcttgactt agagattgat
    16021 gctcaaagtg ttttagttgg agtttatcca ttcattagca gctcttcaga aggagaagac
    16081 gagatcactc taccagaaga agttattttc acggattacg tggatgatta tcctgctgga
    16141 tatgtttctt ttgttgattt taaagacaaa gcgactgatg tagccacatt aagggaagct
    16201 gctaaagact ggttgaaaac aaacatagat aaacaaaaac cacaagtgag tggttcgatt
    16261 gaattagtac cattgaggca tcaaagaggc tatgaaaaat ttgttgatct agaaaaagtt
    16321 tcgatgggtg acggagtaga tgtgtatcat ccacagttaa aagtgaatat gtcagcgaga
    16381 attgtggaat atacgtttaa tgttctaacc aattcatacg ataaattagt tgtaggaaac
    16441 gtcaaaacaa acttcttaga aaacacagag aataatgtca gcaatttgat taatgatgcc
    16501 attgatcaat tgaaaaacgg tggcgaaatc agtgatttac tcaatgatat tgtagatcat
    16561 caaactgata tgattactgg tcaaaatggt ggttatgttt tattagatcc taaagaagcg
    16621 cctagtcgta ttttgattat ggacacacca gataagaata ccgcaaggaa tgttttacaa
    16681 atcaacaatg ctggtattgg tttttctaaa actggcatta atggaacata tgaaacggca
    16741 tggacgttag atggcggatt caatgcctcg tttattacgg ctggtgaaat agtaggaatt
    16801 actattagag gtactacatt aattagtgat ggtgctgatt atagaacaag tattgctaat
    16861 ggcaaaatga cttggtactc aaaaaaagtt aacaaagata ttatggagct agaagcacgt
    16921 gattatgtaa gtgctgatgc cggtattgta tcatacacca tgaaaactgg tggtggtttc
    16981 atgattagaa atccacaggg taacttggtt tttagtacgt gggataatgg taataacaga
    17041 ccgtttctat cttttggtgc gcccaatttc aggtatagca atgctagtta tgtaacttct
    17101 ggcgacggta gttctttaag cattaatggt agtgcgggta actcatggga atttaaggta
    17161 gctggtagga ctatgaaatt tactagtgat ggtatgctaa cgttaccagg ttgttttttt
    17221 ggttcatggg aagatgggaa acttgctagg tttgaacaat caacggtaca agtatataaa
    17281 gattttactg ttagaggtac taaaaactca actgtaccaa cagaacatta tggacaacga
    17341 ctattgaacg cttatgaaac tccagaatat tatttcgctg attatgggga agccgttaca
    17401 ggtgacaatg gtaaagttcg tgttgatatt gaccccatgt ttgctgaaac agtaaatcta
    17461 agtcggtata tgacacatgt gacacctaca gaactagttt tgtgtgctgt tactcatgaa
    17521 gatattgacc atttcatcat tgaaactagt aagccaaacg tattagttag atggaattta
    17581 gtggcacacc gtctagggta tgaagatatt agattaaaag aggatacagc atatgatagc
    17641 acagtgcttg accaaaaacg tttttaaaac gaagacaagg aggtatataa atggctagca
    17701 gtttatataa tttggcttta gatttcagca aagaattaaa ctacaccaaa gctattatgg
    17761 ctcgtcaagg tgataaaggg attacggtga cggttaaacc gtttctaaat ggcttgcaga
    17821 tggatacgag tggcggaaca tttactttaa aaggaacaac accatctaac cgttacgtag
    17881 ataatgttgc aactagtgta actagtgaag aagtcacgtt ttctcttgat ggcacattta
    17941 tgagtgaagc aggatattat aaacactgct acgtagaata tagaaaagac aatcaaattt
    18001 taacaacgca agatatcatt tttttctcac taggagtgtc tgacatttcg caaggccaag
    18061 ccgatgaata tgtttcgcaa ttagaagagt tgattcgaaa gtacaacgaa acttttgatg
    18121 cttttatggc tgaaatcaaa ggtagagtgg atagcttaaa tcaacagatt actgatttaa
    18181 ctggtcaagc taaaacgcta caagacaagt tagatgctct gaaagaagaa atttctaagt
    18241 taggtaactt acaagtgatg tacagtaaca gcatcgactt cggggactat gattattcgg
    18301 agaacccgaa tttaatgcca tatattacag aaccttgggt tggacctttg ttaggtaatg
    18361 gacatacagt taaagatagt gttaaaaggg ttataactca tactaaaact agaacagcta
    18421 attccggcga catattatcg cttggcttag gtattccgtg tacagcggaa gctaataata
    18481 ggtatcttat aaccacttta cgaccatcta cgacatacac attaagcgtt actatgagtg
    18541 tcggttcaga ttggactgga gagaccaata ctataggggt taggcttaga tatttaaatg
    18601 aacaaggtgg tatagaactg cccattaatg cattaatacc agcaaatgta gaacgcgata
    18661 aaatggtcac tcatacattt actggtataa caaaagataa tgtaactagc ataacaaact
    18721 gttatgttga aatattctca cttaatagtg aatataaagg aacggttagt gtcagctatg
    18781 acgttaagtt aaaagcacac taccctaact tactagatgg cccttactgg ttaggtaaag
    18841 ttcctttagg cgagaatatt gctgacccta cagttgtctt tccacacaaa actagtgaat
    18901 atatggttta tggtagacgc aatacagaaa actatatcgc tgaccaaaca tataccatat
    18961 caatgaaagc aactaaacta acagttcaat cgtttgccgt gtatattgct gctggccgcg
    19021 ttaaagtagg agacatgaag cctactgagg ggttagctaa tacatgggag ttaactttta
    19081 ctgtaactaa gcaacatata gatagtgggg taactaatta tctggaaata taccaatacc
    19141 cgtcagctac aaaaggagct gttcaaattg agtggctcaa actagaaaaa ggcaatactc
    19201 gaaccccgaa tattagtgag tataaatatc gtggtactgg tatgcgtgat tcaaacaatc
    19261 caaaagatta tgtttgggat ctagcaccag aatatgtcga agataatttg gccacagata
    19321 ttaaaatttc tgaaattact ggtaaagcaa acaattatac cgatgggaaa gtatcggaga
    19381 ttaattcgca gttgactgct tcaattaatg aagtagacac cacagctaag gatgctcaaa
    19441 caaaagcgaa tgctaatgcg actgctatag atgaattaga caataagatc gatgaacgca
    19501 ttaatgatac agctactacc acattaacag ttacaaacgg gaataccgga tcagcaaagc
    19561 tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga aaaagcagtg
    19621 gtggaaatga ttcaacgata ctaacaattc cagaaggcta tcggccacca attagttttg
    19681 agcaactggt tggctcgata gaccgttcta ctttgaacag tgctcagtta tctattggtg
    19741 cagatggagc cattaaatgg cgaagaaact caagttatgg atcggattat acctttgcaa
    19801 ttacttacac gatttagaaa agcgtgaatc gatatgaagg cagcatatag accaattgaa
    19861 ccttacggat tcgagcaaat cattgtgaat gatgaagaac atttaccgga agaatgcaca
    19921 gaagtcgaac caccgattcc aaattggaaa ccgaaattca attactggga gggaaataaa
    19981 tgaaaaacat ttggaaatat ggacgtactg gcggagagta cgcaggaaaa gtattggacg
    20041 acatgcttgt atccgttcct tacacagatc agccaccgct tgaaggaatt cgtgctgatg
    20101 gcgaaccgtt aacaatcgct gatcaaatgt ttgatcctaa attgaatcaa tggattattt
    20161 tagcgaacgc actagatcac aacgatttaa acaatctcaa agcaatgtat gagtcgttag
    20221 aaaatgagaa cggcgattta aaacagatca atgccaaact catgctaagc gatgtagcaa
    20281 ttaaacagga aaatactgca ttgaaagaaa aagcggatag tttagcacaa atcaattcaa
    20341 aaatgatgct tgcttcgtta caaaatagca aagacatttc agaaattaaa gagcaactaa
    20401 atccagcttc aaagggaggt gagtagtatg tttagtttta gcgatgtgaa aatgatgtat
    20461 gattggggct gtttcactaa cgaacaagta atggttttcg ttccgttgtg cattactgaa
    20521 gaaaaagcag ataaaatcat tagcaaagaa gagagcgcat cttaattgat gtgcttttta
    20581 ttttgattca aggagttgtc acatgattaa tttaggggaa tggggagcga tagcaggatc
    20641 aataaccgct atcgtttctt tgattttatt agtaataaaa ccaattactg catctttctc
    20701 gaagattact gagactcttt caaaagtaag tcacaattta gatttgctga ctaaagattt
    20761 agaatcgagc aaatcagatc gattgatgat tcatgaagaa ctaaagaaac acgatgaaag
    20821 attagataca catgcagaaa aattggtaga acacacgcaa caaattaaaa ctttatttag
    20881 ggagagaaga aaatgaataa taaaacgttc gaagtactaa aatggttcgc actggtaatt
    20941 attcccgcac tagctacttt cgtggggtta gttggtaaag cgctcaattg gcagtacaca
    21001 gatatctgtg ttgtcatcat tactggtttt ggcgcgtttt tagggagtgt gttgggtgta
    21061 tcaaatcgaa cctacaaaat gttctcggct gaaagcgaag aaggaggaaa caaatgaaaa
    21121 agaaaattac tattactgcg atgagcctgt taacggctct ttttttatta cccattaata
    21181 cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga gactccagat
    21241 tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca gcaagaaacg
    21301 tagccgccaa catgaaa
//
LOCUS       DACEKG010000048        21309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEKG010000048 DACEKG010000000
VERSION     DACEKG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.049"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..808
                     /locus_tag="IW664_001729"
     CDS             68..808
                     /locus_tag="IW664_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0849091.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IW664_001730"
     CDS             complement(1232..1402)
                     /locus_tag="IW664_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0849092.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IW664_001731"
     CDS             complement(1596..1775)
                     /locus_tag="IW664_001731"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849093.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1785..2009)
                     /locus_tag="IW664_001732"
     CDS             complement(1785..2009)
                     /locus_tag="IW664_001732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849094.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            2162..3274
                     /locus_tag="IW664_001733"
     CDS             2162..3274
                     /locus_tag="IW664_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0849095.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="IW664_001734"
     CDS             complement(3352..3645)
                     /locus_tag="IW664_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0849096.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="IW664_001735"
     CDS             3813..4127
                     /locus_tag="IW664_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0849097.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="IW664_001736"
     CDS             4148..6127
                     /locus_tag="IW664_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0849098.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="IW664_001737"
     CDS             6140..6466
                     /locus_tag="IW664_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0849099.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="IW664_001738"
     CDS             6482..7000
                     /locus_tag="IW664_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849100.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="IW664_001739"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="IW664_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0849101.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="IW664_001740"
     CDS             complement(8436..9941)
                     /locus_tag="IW664_001740"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0849102.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="IW664_001741"
     CDS             complement(10033..10623)
                     /locus_tag="IW664_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0849103.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="IW664_001742"
     CDS             10802..12448
                     /locus_tag="IW664_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0849104.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="IW664_001743"
     CDS             12497..13216
                     /locus_tag="IW664_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0849105.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="IW664_001744"
     CDS             13695..14642
                     /locus_tag="IW664_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0849106.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="IW664_001745"
     CDS             14654..15574
                     /locus_tag="IW664_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0849107.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="IW664_001746"
     CDS             15615..17072
                     /locus_tag="IW664_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0849108.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="IW664_001747"
     CDS             17417..18031
                     /locus_tag="IW664_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0849109.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="IW664_001748"
     CDS             18024..19748
                     /locus_tag="IW664_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0849110.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="IW664_001749"
     CDS             19745..21196
                     /locus_tag="IW664_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0849111.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6869 a         3609 c         4317 g         6514 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat attcatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgg
//
LOCUS       DACEKG010000049        20004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEKG010000049 DACEKG010000000
VERSION     DACEKG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.051"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            324..1985
                     /locus_tag="IW664_001750"
     CDS             324..1985
                     /locus_tag="IW664_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0849112.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2055..2402)
                     /locus_tag="IW664_001751"
     CDS             complement(2055..2402)
                     /locus_tag="IW664_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0849113.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2486..3427
                     /locus_tag="IW664_001752"
     CDS             2486..3427
                     /locus_tag="IW664_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0849114.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3428..4471
                     /locus_tag="IW664_001753"
     CDS             3428..4471
                     /locus_tag="IW664_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0849115.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4477..5547
                     /locus_tag="IW664_001754"
     CDS             4477..5547
                     /locus_tag="IW664_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849116.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5537..6490
                     /locus_tag="IW664_001755"
     CDS             5537..6490
                     /locus_tag="IW664_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0849117.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6502..7512
                     /locus_tag="IW664_001756"
     CDS             6502..7512
                     /locus_tag="IW664_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0849118.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7797..8618)
                     /locus_tag="IW664_001757"
     CDS             complement(7797..8618)
                     /locus_tag="IW664_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0849119.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8715..8993)
                     /locus_tag="IW664_001758"
     CDS             complement(8715..8993)
                     /locus_tag="IW664_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849120.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9261..11270
                     /gene="metG"
                     /locus_tag="IW664_001759"
     CDS             9261..11270
                     /gene="metG"
                     /locus_tag="IW664_001759"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0849121.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11749..12336)
                     /locus_tag="IW664_001760"
     CDS             complement(11749..12336)
                     /locus_tag="IW664_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849122.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12511..13833
                     /locus_tag="IW664_001761"
     CDS             12511..13833
                     /locus_tag="IW664_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0849123.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13988..14764
                     /locus_tag="IW664_001762"
     CDS             13988..14764
                     /locus_tag="IW664_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0849124.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14757..15326
                     /gene="rnmV"
                     /locus_tag="IW664_001763"
     CDS             14757..15326
                     /gene="rnmV"
                     /locus_tag="IW664_001763"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0849125.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15319..16203
                     /gene="rsmA"
                     /locus_tag="IW664_001764"
     CDS             15319..16203
                     /gene="rsmA"
                     /locus_tag="IW664_001764"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0849126.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16654..17283
                     /locus_tag="IW664_001765"
     CDS             16654..17283
                     /locus_tag="IW664_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849127.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17407..18516)
                     /gene="ugpC"
                     /locus_tag="IW664_001766"
     CDS             complement(17407..18516)
                     /gene="ugpC"
                     /locus_tag="IW664_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0849128.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18725..19147
                     /gene="mgsA"
                     /locus_tag="IW664_001767"
     CDS             18725..19147
                     /gene="mgsA"
                     /locus_tag="IW664_001767"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0849129.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19470..>20004
                     /locus_tag="IW664_001768"
     CDS             19470..>20004
                     /locus_tag="IW664_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0849130.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6509 a         3594 c         4080 g         5821 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag
       61 tttctttttg tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat
      121 tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa
      181 ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat
      241 tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa
      301 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
      361 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
      421 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
      481 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
      541 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
      601 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
      661 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
      721 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
      781 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
      841 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
      901 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
      961 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
     1021 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
     1081 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
     1141 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
     1201 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
     1261 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
     1321 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
     1381 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
     1441 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
     1501 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
     1561 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
     1621 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
     1681 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
     1741 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
     1801 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
     1861 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
     1921 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
     1981 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
     2041 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
     2101 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
     2161 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
     2221 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
     2281 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
     2341 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
     2401 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
     2461 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
     2521 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
     2581 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
     2641 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
     2701 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
     2761 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
     2821 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
     2881 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
     2941 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
     3001 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
     3061 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
     3121 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
     3181 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
     3241 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
     3301 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
     3361 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
     3421 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
     3481 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
     3541 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
     3601 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
     3661 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
     3721 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
     3781 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
     3841 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
     3901 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
     3961 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
     4021 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
     4081 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
     4141 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
     4201 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
     4261 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
     4321 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
     4381 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
     4441 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
     4501 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
     4561 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
     4621 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
     4681 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
     4741 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
     4801 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
     4861 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
     4921 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
     4981 ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact
     5041 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
     5101 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
     5161 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
     5221 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
     5281 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
     5341 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
     5401 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
     5461 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
     5521 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
     5581 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
     5641 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
     5701 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
     5761 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
     5821 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
     5881 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
     5941 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
     6001 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
     6061 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
     6121 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
     6181 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
     6241 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
     6301 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
     6361 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
     6421 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
     6481 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
     6541 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
     6601 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
     6661 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
     6721 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
     6781 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
     6841 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
     6901 cagcatcggc cagttgaagg atctattcgc tccgctacga tttctgctcg atataatgaa
     6961 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
     7021 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
     7081 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
     7141 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
     7201 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
     7261 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
     7321 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
     7381 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
     7441 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
     7501 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
     7561 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
     7621 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
     7681 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
     7741 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
     7801 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
     7861 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
     7921 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
     7981 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
     8041 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
     8101 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
     8161 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
     8221 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
     8281 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
     8341 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
     8401 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
     8461 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
     8521 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
     8581 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
     8641 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
     8701 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
     8761 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
     8821 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
     8881 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
     8941 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
     9001 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
     9061 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
     9121 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
     9181 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
     9241 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
     9301 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
     9361 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
     9421 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
     9481 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
     9541 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
     9601 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
     9661 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
     9721 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
     9781 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
     9841 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
     9901 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
     9961 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
    10021 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
    10081 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
    10141 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
    10201 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
    10261 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    10321 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    10381 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    10441 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    10501 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    10561 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    10621 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    10681 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    10741 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    10801 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    10861 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    10921 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    10981 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    11041 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    11101 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    11161 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    11221 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    11281 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    11341 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    11401 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    11461 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    11521 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    11581 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    11641 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    11701 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    11761 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    11821 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    11881 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    11941 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    12001 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    12061 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    12121 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    12181 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    12241 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    12301 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    12361 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    12421 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    12481 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    12541 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    12601 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    12661 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    12721 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    12781 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    12841 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    12901 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    12961 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    13021 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    13081 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    13141 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    13201 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    13261 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    13321 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    13381 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    13441 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    13501 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    13561 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    13621 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    13681 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    13741 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    13801 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    13861 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    13921 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    13981 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    14041 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    14101 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    14161 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    14221 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    14281 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    14341 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    14401 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    14461 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    14521 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    14581 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    14641 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    14701 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    14761 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    14821 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    14881 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    14941 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    15001 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    15061 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    15121 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    15181 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    15241 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    15301 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    15361 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    15421 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    15481 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    15541 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    15601 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    15661 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    15721 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    15781 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    15841 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    15901 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    15961 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    16021 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    16081 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    16141 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    16201 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    16261 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca
    16321 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    16381 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    16441 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    16501 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    16561 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    16621 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    16681 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    16741 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    16801 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    16861 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
    16921 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    16981 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    17041 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    17101 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    17161 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    17221 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    17281 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
    17341 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
    17401 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
    17461 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
    17521 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
    17581 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
    17641 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
    17701 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
    17761 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
    17821 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
    17881 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
    17941 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
    18001 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
    18061 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
    18121 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
    18181 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
    18241 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
    18301 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
    18361 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
    18421 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
    18481 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
    18541 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
    18601 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
    18661 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
    18721 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    18781 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    18841 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    18901 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    18961 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    19021 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    19081 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    19141 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    19201 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    19261 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    19321 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    19381 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    19441 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    19501 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    19561 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    19621 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    19681 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    19741 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    19801 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    19861 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    19921 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    19981 gaagtttagc gatccacgct aaat
//
LOCUS       DACEKG010000050        19850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEKG010000050 DACEKG010000000
VERSION     DACEKG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.052"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW664_001769"
     CDS             <1..111
                     /locus_tag="IW664_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0849131.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            124..510
                     /locus_tag="IW664_001770"
     CDS             124..510
                     /locus_tag="IW664_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849132.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1061..1285
                     /locus_tag="IW664_001771"
     CDS             1061..1285
                     /locus_tag="IW664_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849133.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1303..1731
                     /locus_tag="IW664_001772"
     CDS             1303..1731
                     /locus_tag="IW664_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849134.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1709..3127
                     /gene="terL"
                     /locus_tag="IW664_001773"
     CDS             1709..3127
                     /gene="terL"
                     /locus_tag="IW664_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ0849135.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3139..4674
                     /locus_tag="IW664_001774"
     CDS             3139..4674
                     /locus_tag="IW664_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0849136.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4754..5632
                     /locus_tag="IW664_001775"
     CDS             4754..5632
                     /locus_tag="IW664_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0849137.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5634..5951
                     /locus_tag="IW664_001776"
     CDS             5634..5951
                     /locus_tag="IW664_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849138.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6000..6701
                     /locus_tag="IW664_001777"
     CDS             6000..6701
                     /locus_tag="IW664_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0849139.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6716..7606
                     /locus_tag="IW664_001778"
     CDS             6716..7606
                     /locus_tag="IW664_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ0849140.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7629..7844
                     /locus_tag="IW664_001779"
     CDS             7629..7844
                     /locus_tag="IW664_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849141.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7856..8197
                     /locus_tag="IW664_001780"
     CDS             7856..8197
                     /locus_tag="IW664_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849142.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8197..8568
                     /locus_tag="IW664_001781"
     CDS             8197..8568
                     /locus_tag="IW664_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849143.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8561..8956
                     /locus_tag="IW664_001782"
     CDS             8561..8956
                     /locus_tag="IW664_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0849144.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8958..9335
                     /locus_tag="IW664_001783"
     CDS             8958..9335
                     /locus_tag="IW664_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849145.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9336..9944
                     /locus_tag="IW664_001784"
     CDS             9336..9944
                     /locus_tag="IW664_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0849146.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9944..10285
                     /locus_tag="IW664_001785"
     CDS             9944..10285
                     /locus_tag="IW664_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849147.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10527..13316
                     /locus_tag="IW664_001786"
     CDS             10527..13316
                     /locus_tag="IW664_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ0849148.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13306..14046
                     /locus_tag="IW664_001787"
     CDS             13306..14046
                     /locus_tag="IW664_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0849149.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14043..16805
                     /locus_tag="IW664_001788"
     CDS             14043..16805
                     /locus_tag="IW664_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0849150.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            16818..17726
                     /locus_tag="IW664_001789"
     CDS             16818..17726
                     /locus_tag="IW664_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0849151.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            17726..18343
                     /locus_tag="IW664_001790"
     CDS             17726..18343
                     /locus_tag="IW664_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849152.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            18347..18796
                     /locus_tag="IW664_001791"
     CDS             18347..18796
                     /locus_tag="IW664_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849153.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            18796..19320
                     /locus_tag="IW664_001792"
     CDS             18796..19320
                     /locus_tag="IW664_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849154.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            19348..19755
                     /locus_tag="IW664_001793"
     CDS             19348..19755
                     /locus_tag="IW664_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849155.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            19755..>19850
                     /locus_tag="IW664_001794"
     CDS             19755..>19850
                     /locus_tag="IW664_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0849156.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT         7089 a         2788 c         4213 g         5760 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agcatacaaa ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata gggggaccat
      121 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
      181 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
      241 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
      301 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
      361 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
      421 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
      481 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
      541 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
      601 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
      661 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
      721 attatctatc atccgttgct gtctattgtt acatacagat cgcttaggcg gtctttttat
      781 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
      841 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
      901 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
      961 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
     1021 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
     1081 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
     1141 tgttcaaatg aaggcgaagt attcgattgt agaaatgatc agagaggcaa cgccagtaga
     1201 gattgattat tgcaagctag tctattgtgg gtgcggccgt tggaaagagg atcatgtaca
     1261 aaaaaatatc agcaagtaca tatagagagg cggtgattaa ggatggaaaa aagcgatacg
     1321 aaagcgaaca gatacaaacc tactgctgca gagaaaaaac tcttggaagt attgataaat
     1381 ccagataact taggcaaacc ggtgcaagag ttgtgttcac tggcgaaaat aggtaggacc
     1441 aaatattacg acgccatgag caaagatggc tttgttgatc tagttaacga aacaacaatg
     1501 gatctgatca aaggaaaagc ttctgatgtc cttaatgcca cttacaagta tgcattgact
     1561 gaaaaaggtc atcaggatag gaaaatgatt ctgactattg ctggaatcta tgcagacaaa
     1621 caagaactca aacattctgg cggcgtcgat atccgaaaac agtatgagaa aatgtccgat
     1681 gaagaattag aagagttggt gaaacggtat gaaaaaatca acgactctta atcgtgttga
     1741 gtatgccgtt gttcaaagag agctagccat tcgaaaagct aggaaaagtt tttggctatt
     1801 ctgccagctt ctttatccag agttttacaa agatgatcgg ttgtatttga aagatttatg
     1861 tcatacgcta caagattttt acgaagataa aatcgacaaa aaaattttgg ttattaacat
     1921 gccgcctcga catgggaaga cttttacagc gcggctattc gttttatgga tgtttggaca
     1981 aaatccaaag acgaaaatta ttaccggatc ttataaccaa atcctgtcat ctttatttgc
     2041 gcaacaaaca cgtgatggaa tcatggttga aaacgaaaat gttaaggctg aatactttaa
     2101 agatattttt ccagaggtgc aaatcaaata tggagatgct gcaaagggtt tctggtcttt
     2161 agatggttca gaagaaaaga attatttagc gacttctcct ggtggaacat ccactggtat
     2221 tggcgcgaat tatgtaattg ttgatgacat cattaaaacc gcagaagaag cctataacga
     2281 gcgtgtttta gattctcact gggaatggta taacaacacg ttggcccaac gaatggaacg
     2341 gccacgtaaa cagatattga ttatgacacg ttggtcaagt aatgatttag ccggaaaaat
     2401 gttgacgaga cgaaaaaata atgttcatca gatctgttat aaagcagtac aagaagacgg
     2461 ctcgatgttg tgcaacgaga ttatgaccca cgatgagtac ttagatgtgg tacaagaaat
     2521 gaatgtagat attgcagaag ccaactatca gcaagaacca attgaccaaa aaggcaggct
     2581 atatcagaag tttcttactt atgatacgtt gcctgataac attatcaaaa tatggaacta
     2641 caccgacacc gccgacaagg gtgccgatta ctttgcgtct cctgtttttg ctgagacgtc
     2701 tgatcatgat gcttatatta tcgatgtcat gtatacaaaa gagccgatgg aaacaactga
     2761 aatagcccac gctaatatga ttattcgcaa tcgagttaat aaggtacgga ttgaaggaaa
     2821 caatggcggg cgtggattca gaagaaattc agaaagaatt acaaaagagc gaggctatcg
     2881 aggtgcagta tttgaagact ttcatcaatc ggataataaa ctgtctagaa tactggcgaa
     2941 tagtgcttgg gtggaaaatc atgtttacta tccagatgat tgggcgacac gttggccgga
     3001 cttttatgag gctatggtta gttatcaacg tgaaggtaag aacgctcatg atgatgctcc
     3061 ggatgcagtt acaggaattg cagaaacgat tatagaagca gatgacttcg agtttattgc
     3121 ttattaggag gtgggtcttt gttttattct ggaatcacat tgaaaaaata caaacgttta
     3181 agaacgaaat attcaacgca aatcgaagaa ggattattcg atcctaacgc ttttatggaa
     3241 gatatgaaac cctttttcga tgatcgagaa agaaagtatc ttgcttatac tagcgagaaa
     3301 aacgagatcg acagtcgtga gaaaccaaaa acaaaaatca ttaaggtcaa taacaagatt
     3361 catgcgggaa tgtattcagt ggtcgttgat caagcagtta accatttcac aggaatacca
     3421 attaaatggg attatgatgt ttcggaacag aaaaggacat tcattcaacg aatgagagac
     3481 aaattactgg gtaatgatat cagcgcccct tctaccccag aagaatttga aacactcacg
     3541 agacaattaa gtactatgcg gtttggaatg ttagaccccg atactgcaac atttcaagga
     3601 gcgacagggc ttgctttcag attactggag cctgttgaga caaatgatgg ttgggaacta
     3661 agagcaagca acattgagcc gtggaaagct gaaaagtacg aaaacggcgc agtgtacatt
     3721 aaagagaaat acgatccata tcaaaagaag ttttatgaag agatgaaggt tgtcacgccg
     3781 aaatcaatat ttacttatgc tcgatatact gattaccctg gcacggtaca aggagcattt
     3841 aaaacgctgg gtaatgttcc taatccttta ggcgttatca atcttacgga gtttaaaaac
     3901 aatacaaacc gttactgtga ctttgaagtt gcagaggaga ttagtgatgc tattgatcgt
     3961 gttttatctg atcagcagaa cgagattgaa caattcaagt tagcttatat gctgatcact
     4021 ggggcagatt tgtccgagga taaagccaag aagatgatgg agcaactagg gatcatcaat
     4081 ctgaaagacc ctaatgcgaa agccggctac gtgacaaagg atctacaaaa ggatttcaac
     4141 gagtatcatt ttgatcttct taaaaagcaa ttctacacgg tgtgtaaagc aattgatttc
     4201 aatgacgaag tctttaagtc gaatagttct ggggaagccc gaaagtggca aatcattagt
     4261 ttagaagcta agacaaatac caaagaacag tactttcgag aaggattgaa agagtgtgcc
     4321 gaggttatgg ctgcttattt ggattttgcc gagaaagtta caattgatcc ggaaaagatc
     4381 attttcacct tctctcgttc gcttccaaca gatttaagct acctagctga ggcattgcct
     4441 aagttggcac catacgtttc taagaggacg attcaaagtc agatcccgtt cgtcacagat
     4501 gttgattatg aaaacgaaat gattgacgtc gaaagcagac aatcttaccc agacggcgag
     4561 tatggaggtg gcatcgttga gtcaaataga aacgaagtac tggaccaaac gtcgagaact
     4621 ggaagattta gcaagactca aaatggaaaa tcaaacgcta aagaaactaa gtagtgtctt
     4681 tcctgatgcg ttgaaagaaa ttcaagcgga gttattatcc caagctgatt tacacgatat
     4741 tacctatgct gagatgatgg aattttatag tacaagtgat cagaaaaaat atcgtgaata
     4801 cgttgaaaag aactacaaat cactgaagat gtatgatgca aaatacaaag agttcatcga
     4861 agaatacttt ccttctttcg actacgccaa agtgaatcgg ctgcttcaaa tgaggtcaga
     4921 tatttttcga attcttgcag agagtgcaat agatgcagat gtgaataaat atttttcgga
     4981 tcggttagaa gatatcttgc aacgttcgta tgtatcaaac gcaaacgtat ttgcccagat
     5041 tttgaacgta gatttgccga attacttatc aaaggaagaa cttgaacgtt acctgaacta
     5101 tccttggtca ggtaagacat tctcgcgtcg tttatggggc aatatttcat ctctagaaca
     5161 aaagctatct aacgccatta caactagtgt agcaagtggc gaaggagttc tcgtggcatt
     5221 acgaacaatg cgagaagacc cagaaatatg cgatatgttc aaaagggagg aatcgaagta
     5281 caacaaggcg atcgaaaatc ttgtgagaac agaatacgct aaattcgctc aagatggtat
     5341 tgagaaatct tatattgaaa ctggcgtaga cgaatacaat gtcttaactg caaaagatga
     5401 aaaggtatgt tcaatatgcg gtggcaaagc aaagaacaac ccatacaagt tgagtgaagc
     5461 gatcattggt gagaaccgtg cgccttttca tggccgatgc agatgtacag atgttcctaa
     5521 tatgccgaaa ttaggaaaag atattgacga agagtatgag cgtttatttg gcgatttatt
     5581 agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt agaatgaaag
     5641 atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa gcctacaaaa
     5701 cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat ggcaattatg
     5761 cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca gttgataaga
     5821 ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta aaagaaacag
     5881 ttgattggta tgaaaatatg ggatgtattg ttgttagaac tgactacaaa gaaggaaagt
     5941 ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca atactgggct
     6001 tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat
     6061 cgtggggcga aaggagaatg attatgaaac acaaatcatt aatgccaatg aatttgcaat
     6121 actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct tttgtggaat
     6181 ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc gacaaacaat
     6241 tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc aaggaatatg
     6301 aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta caagctgaat
     6361 ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa
     6421 ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt atgctaaata
     6481 atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt acaagtgttt
     6541 tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg aagtctgcat
     6601 ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca ttaacaaatc
     6661 cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa aaattatgaa
     6721 aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta atgctatgca
     6781 agtaccaaat acaccttttt taagctattt acttggtgct ggaaaaacag agccagcaaa
     6841 ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt ctgaaaaact
     6901 tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca actatactga
     6961 aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg tgaatggagt
     7021 cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa
     7081 ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa
     7141 tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg cgggtgcagt
     7201 aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt atatgggaga
     7261 aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt tagatggaaa
     7321 atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa ttggaaatat
     7381 cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa aaggaacaat
     7441 tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga gagcagaaga
     7501 acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact ctcttgaata
     7561 caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg gagttagtac
     7621 tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa aaagatgaag
     7681 tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat cctaaaactc
     7741 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
     7801 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
     7861 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
     7921 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
     7981 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
     8041 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
     8101 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
     8161 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
     8221 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
     8281 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
     8341 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
     8401 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
     8461 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
     8521 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
     8581 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
     8641 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
     8701 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
     8761 aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat atgtagaaga
     8821 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
     8881 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
     8941 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
     9001 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
     9061 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
     9121 tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat ttaattaatg
     9181 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
     9241 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
     9301 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
     9361 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaatttcc
     9421 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
     9481 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
     9541 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
     9601 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
     9661 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
     9721 gacagtttgc tctagattca gatgaaacgt caacgattga aggaagtaaa gtaagtttta
     9781 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
     9841 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
     9901 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
     9961 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
    10021 tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg aagaaatgca
    10081 aacagcgact gataaacaat cagttaaaaa agctttaagt cgtgcttatt catttattgc
    10141 agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa ttgatgctcg
    10201 tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt ttgatgaaac
    10261 ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt taagtattcg
    10321 attacttacc gagaattaga tataaagacg caattactcg aagcaggttg gacgttacca
    10381 gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag agatgcggta
    10441 aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga ggtgatactt
    10501 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
    10561 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
    10621 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
    10681 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
    10741 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
    10801 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
    10861 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
    10921 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
    10981 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
    11041 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
    11101 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggtttg tctcaaggta
    11161 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
    11221 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
    11281 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
    11341 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
    11401 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
    11461 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
    11521 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
    11581 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
    11641 tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc gttggtgcaa
    11701 tagctgtttt cggttacttt atggcaacca atgaagagtt tagaaacaag gttatatcta
    11761 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
    11821 ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt gtcaaagatg
    11881 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
    11941 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
    12001 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
    12061 gagaaaaatg gaatgaaatt aaaggttcta ttatgtcggt tgctggtcca ttgattacag
    12121 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
    12181 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
    12241 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
    12301 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
    12361 gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc aagcttacat
    12421 tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta aacataaagt
    12481 atttttttat taatctttgg attgatataa aatattttgc aattcagaaa tggattgaat
    12541 tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt actacttgga
    12601 ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg gcatacaata
    12661 cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag gactctttct
    12721 ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat ctaaaaaatg
    12781 gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg gaagtcaatt
    12841 tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa gaaaagtgga
    12901 atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg attaaaggag
    12961 ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa aatattgttc
    13021 aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc atgacaaatt
    13081 tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta ccaattacac
    13141 aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat ttaaatgttt
    13201 atggagattt acctgattca atggttcgga agattaccac aaaaattaaa acagaattga
    13261 caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca acttaagaga
    13321 ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat tcaaaacagg
    13381 cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg aaatgattct
    13441 atcattattg ataaaggcta ttacagaaat gtgactagaa agattgagtg ttactataaa
    13501 gcaccttcaa ttgatgtggt gcaggagtgg gaagatcgaa tcactgaatg gttagatatg
    13561 tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca agctgtagta
    13621 atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc ttttgaattt
    13681 acagtgagca ttcgaccatt taaggaaaat tacaatggta gatttactat tcgacaaaca
    13741 gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat taaattgagt
    13801 ggttctggag atgcttcttt ttatattaat aaggacaaat atgatttgaa atcgttggac
    13861 agagaattat ttatagattc taaattagaa gaggcttacc gaaaattaga tggtaattta
    13921 gaacatcagg atcaagtcac tttattttta gattttccat ttttatatcc tggaaaaaat
    13981 gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg gtggagaaaa
    14041 gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat ggcttaggaa
    14101 ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa tatgaattag
    14161 agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac tatcaaataa
    14221 aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat aaagatatag
    14281 atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga cttggaagac
    14341 gggtagtcac tcttgcggaa gtagactcta aaactggtca agaagcgata tcaattattg
    14401 aaaccaaaat ggataaaaaa tctgacatac gactttattc tgatattacg gccgtttcta
    14461 gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa ggatcattgc
    14521 ttcaatattg gggtggagaa attaaacgtg aaccatttaa gctttctttg ttaaagcgaa
    14581 gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt ttaaaggtca
    14641 aattagattg gacaggtata aaaacaagac ttattccata tgctgatcct caaagtgagg
    14701 taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt aataactatc
    14761 ctgatgtgta tacagagcat gttcagttta cagaagaaca aggagtaaaa gacattaaga
    14821 gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt gataagccta
    14881 aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg aaagaatttg
    14941 cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa aaatataata
    15001 tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa aaaactttga
    15061 aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta actattcagg
    15121 ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat tatgtttctt
    15181 caatgattac aggtcaaggc agtgcaggag gatatgtaag tttatggcca aaagaaaaac
    15241 cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa gtactaagaa
    15301 tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt acttcagctt
    15361 ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc aaagcagata
    15421 tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat ggactactcc
    15481 aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg gaattttgga
    15541 attctaaggg ttcaattgga acgattggaa caactgattc tgctggtaat ccttttcctg
    15601 gggcttctac tccaacccct attgaagata attctttagt tattcgtaca aatggagacg
    15661 gcaaatatat tttgatttct cctaaagtag gaaaaggatt agttttatta ggaaatggta
    15721 aagcaattta ttttggagac ttagatgtac aaggaaaact tacagttaaa ggaaaagaaa
    15781 taattcctgg acaaggtggt ggtccatcgg gaggtggaga atctactggt gggtatccta
    15841 gtgaattaaa aacagatgca gaaaagagag cttggagaat ttacgatatt ttgtgtaata
    15901 acggatttac gaaacaatct gcatgtggaa tattggggaa cattcaacaa gaaacaggag
    15961 gaacttttga tcctgatact gttcaaatag gtggaccagc atacggatta gttcagtggg
    16021 atggttcttc atatcctctt gttggtccag caacatggga tggaaaagtt tatgttcaaa
    16081 acttatttaa tgctgcaggt attaaagaac caataacgag cttagatgca caagttcgat
    16141 tgcttatttg gacatttaca aatggacaat ggatgggcgt agtacaacct acgacggttg
    16201 atggatttaa ggcctgtact gatccaagac aagcagcata tgcttttgaa cgaaactatg
    16261 aacgtccggc agcgacacat cctgaacgtc aagattatgc agttaactgg tataacaaat
    16321 ttaaagattt aaaaccagga ggagctactg gagaagcagg actaaaacat ttagagtctt
    16381 tgattggaca acgaattggc aatggtcagt gttatggctt gtctgcagaa tattcaggat
    16441 atctgggtgg ttgtggcatg ggtgctggaa caaaatatgg tttaactcat gtgataggaa
    16501 atacttctgc agcatctgat attggtattg cctatgattg gtctgctgtt ggttggaaag
    16561 tgattcaaaa ccctagatat gatcaattag tagttggtgc aattattaat tgggcaagag
    16621 gtggacaagt gggttcatgg tttgcagacg gaacttatgg acatactggc gttatccgag
    16681 gcttagctaa tggtcgtatg caaacttatg aacaaaacac agagttaggt atgatttgtg
    16741 gaaagttaga tcgccagtat tatagtgcaa gtgcaatttc ttccattgtc ataccaccaa
    16801 aatagggagg tgatttaatg gcaaagtgga atgtcatatt aagtacaaca gaaccctata
    16861 attacgtggg gatgattcaa gttcgacaag gcaataaaaa cactgaagtt atggaagcta
    16921 caattgtcga aaatggtctt ccatatgatt tatcagaatg taaggtatat tttgaatcag
    16981 ttgttggcgg aaaatatcca gtccaattag aaacaagaat tgtggatgct aaaaaaggga
    17041 aaatcaaata tatttttgat aaatattcca tgcagtgttt gcatcgacaa acagccaatt
    17101 tcattatatt taaaggagaa gacttgattg gaacaactca agacttctcc tattttgtca
    17161 tcaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca attgaagatt
    17221 taattgctga tatgacagat tttattaatg aaaataaagg cgattttacg gactggatga
    17281 atgaaaggaa agaagaattt gatcgctgga gagaagaaca ggagaatagt ttccaagatt
    17341 ggagagaagg acaagaatca gattatttgt catggtttga atcaatcaag gatattttga
    17401 agtctatcga tccaggtgga gtaatgttag ccgaattaat ggatgcacgt gttgatattc
    17461 aaggagttcg tcatgcgtct atttcagagc gcttgctggc agatatggat tatctatatc
    17521 agaaattacg agcaacactt ttcacgattg aatacggtga gattgaagtg actgatattt
    17581 tgcaggatga tctcttttca gataatcacg aagtcgaaaa agttggaact gtagaatttc
    17641 caattgaaga aggagctttg attattgcaa ctgttgatga tccaaaacag aatgttttca
    17701 ctcttgagaa agttggggtg atttgatggt taaagtaaag agaatgatgg agactgatga
    17761 atctggtgtt aaacgtcaat ttatgccaat tacacatgtt tctgcagttt taggcttaga
    17821 aaaaatgata tcaggacaat caaaagttct ttcggttaat ggtaaatatg gtgcagtcat
    17881 tttaacgaaa gctgatttag gattagagaa tgcaattact gaactacctt atgcaagcga
    17941 gacaagcgat ggtattctta ctgctgaaat gtttcaaaag atcgtaaatg gagaaggtgg
    18001 aacatatatt ttgccgattg ctactcctga acaattagga ggtataaaag ttggggaact
    18061 cttagaagtt actgaagaag gtgttttatc tgcaactaag caaacagact tcaatttctc
    18121 tgaagaatta aagggaaaat tagaatctct aaaaatatta aaagcaggtg caaatatatc
    18181 aattgcagaa gatggaacaa ttagttctac aggtggtagt gggactggtg gtgttaatca
    18241 gtcatatgta gatcaaaaat tccaagaagc tgtaaatcaa gcagaaagtt acacaaacga
    18301 aagaattcca aattttactt ttgaaaaaat cggggaggta tagaaaatga cagatatcgt
    18361 aaaaataaaa caaagcggtg tacaggttta tccacaaact cattggaatg ctatagaagg
    18421 caaaccaaca acggtgaagg gagataaagg cgatcctggc caagcagcaa caattaccat
    18481 aggaaccgtt tctagtggtt caaccgcttc tgttacgaat gtaggaacat catctgcagc
    18541 tagatttaat tttgttttac caaagggaga taaaggtgat cctggaataa atgccacaac
    18601 tacagctgta gctacaacta cagcaaatgg gttgatgtcg tccacagata aaactaagtt
    18661 ggatggaatt gcagctggag cacagaaaaa tccaggtaat gctacaacta cgacagcagg
    18721 tttaatgtca gcaaccgata aagtgaaact cgatggatta accaatatta catttgagaa
    18781 agtggggacg gtttaatggc tgatattgtg caattaaaag aagacggagt tgctaagtat
    18841 ttgaaaactc atgccaaagg aattgatggt gtggagggtg ttttagtaaa agccacaggt
    18901 aatgaaacgg tcttaggaac gaaaaatttt aaagacggat tgcaattcaa tggattgcct
    18961 gtgcaagcag gtatgatcga gcgagctatc acactagctg atcgaagtga tacaacaaac
    19021 gtaactgatg tgaatggaaa aataatcaga atcggaaaca tcgtatttct tacttttaat
    19081 tttaaatgtg gcacatggcc cgaaggatca gaaactcgtt ggattttaaa aatcccggac
    19141 ggtttcaaac gtgatcaagg ctatccagcg caaaccgcgc tttcactagt tagaaatgca
    19201 agtcagccag cggatgcacg tgcattcatt gatcaaagta gcatcataca agccaaatca
    19261 ggttcaggta gttcatatat ttcaggtatg tggatcactc aagatccttg gccggcttaa
    19321 cagattacta attggaggaa gagtaaaatg aaaacaattt ataaagtatt gtatccaatg
    19381 ggatatgaag aacatcaagt agaagataac tttccaacat ctttaccatt tgtcgaagtt
    19441 ccacctattt tgtttgaaaa gaaggaagat gaaacggatg aaggtttcgg aagaagacaa
    19501 cagtcgcaat tctttaactt cactgaaaat aagtgggaag aagcagttac acaagattat
    19561 tcaaaaaaat tagaactact agaaaattta tctataggtt tacaggtaga taatgctgca
    19621 ttgaaaaaat caaatgaaga gttaacggaa aaagcagatt cgatggcgca gttgaacgcc
    19681 aaactgatgc ttaatgattt aacaattaat aaaaaaattg aagcaataga aaaacaaatt
    19741 ggaggtgctt cataatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt
    19801 ttactgacga tcaagttcga caattcgttc cactatgcat tacagacgaa
//
LOCUS       DACEKG010000051        19847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEKG010000051 DACEKG010000000
VERSION     DACEKG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.053"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..362
                     /locus_tag="IW664_001795"
     CDS             168..362
                     /locus_tag="IW664_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849157.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IW664_001796"
     CDS             631..930
                     /locus_tag="IW664_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849158.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IW664_001797"
     CDS             complement(1027..1461)
                     /locus_tag="IW664_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0849159.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IW664_001798"
     CDS             complement(1693..2403)
                     /locus_tag="IW664_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0849160.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IW664_001799"
     CDS             complement(2393..3253)
                     /locus_tag="IW664_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0849161.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IW664_001800"
     CDS             complement(3258..3896)
                     /locus_tag="IW664_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0849162.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IW664_001801"
     CDS             complement(3911..4210)
                     /locus_tag="IW664_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0849163.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IW664_001802"
     CDS             complement(4253..5737)
                     /locus_tag="IW664_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0849164.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IW664_001803"
     CDS             complement(5757..6218)
                     /locus_tag="IW664_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0849165.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW664_001804"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW664_001804"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0849166.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IW664_001805"
     CDS             7521..8291
                     /locus_tag="IW664_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0849167.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IW664_001806"
     CDS             complement(8564..9391)
                     /locus_tag="IW664_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0849168.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IW664_001807"
     CDS             9959..11194
                     /locus_tag="IW664_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0849169.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IW664_001808"
     CDS             complement(12193..12453)
                     /locus_tag="IW664_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0849170.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IW664_001809"
     CDS             complement(12480..13781)
                     /locus_tag="IW664_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0849171.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IW664_001810"
     CDS             complement(14073..14531)
                     /locus_tag="IW664_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0849172.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IW664_001811"
     CDS             complement(14544..15869)
                     /locus_tag="IW664_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0849173.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IW664_001812"
     CDS             complement(15886..17298)
                     /locus_tag="IW664_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0849174.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IW664_001813"
     CDS             complement(17315..18079)
                     /locus_tag="IW664_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0849175.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IW664_001814"
     CDS             complement(18183..19058)
                     /locus_tag="IW664_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0849176.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IW664_001815"
     tRNA            complement(19313..19397)
                     /locus_tag="IW664_001815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IW664_001816"
     tRNA            complement(19415..19486)
                     /locus_tag="IW664_001816"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IW664_001817"
     tRNA            complement(19496..19568)
                     /locus_tag="IW664_001817"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IW664_001818"
     tRNA            complement(19630..19714)
                     /locus_tag="IW664_001818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IW664_001819"
     tRNA            complement(19735..19805)
                     /locus_tag="IW664_001819"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
BASE COUNT         6306 a         4097 c         3083 g         6361 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatag
//
LOCUS       DACEKG010000052        19446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEKG010000052 DACEKG010000000
VERSION     DACEKG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.054"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..3654
                     /locus_tag="IW664_001820"
     CDS             127..3654
                     /locus_tag="IW664_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0849177.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IW664_001821"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IW664_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0849178.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IW664_001822"
     CDS             7442..7783
                     /locus_tag="IW664_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0849179.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IW664_001823"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IW664_001823"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0849180.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IW664_001824"
     CDS             9140..11560
                     /locus_tag="IW664_001824"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0849181.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW664_001825"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW664_001825"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0849182.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IW664_001826"
     CDS             12994..13731
                     /locus_tag="IW664_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849183.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IW664_001827"
     CDS             13745..14560
                     /locus_tag="IW664_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0849184.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IW664_001828"
     CDS             14573..15220
                     /locus_tag="IW664_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0849185.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IW664_001829"
     CDS             15236..15892
                     /locus_tag="IW664_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0849186.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IW664_001830"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IW664_001830"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0849187.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IW664_001831"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IW664_001831"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0849188.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IW664_001832"
     CDS             18234..18752
                     /locus_tag="IW664_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0849189.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IW664_001833"
     CDS             18859..19359
                     /locus_tag="IW664_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0849190.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6539 a         3340 c         4175 g         5392 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggaca
//
LOCUS       DACEKG010000053        19431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEKG010000053 DACEKG010000000
VERSION     DACEKG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.055"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(312..734)
                     /locus_tag="IW664_001834"
     CDS             complement(312..734)
                     /locus_tag="IW664_001834"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0849191.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(748..2154)
                     /gene="atpD"
                     /locus_tag="IW664_001835"
     CDS             complement(748..2154)
                     /gene="atpD"
                     /locus_tag="IW664_001835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0849192.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2177..3079)
                     /locus_tag="IW664_001836"
     CDS             complement(2177..3079)
                     /locus_tag="IW664_001836"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0849193.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3093..4649)
                     /locus_tag="IW664_001837"
     CDS             complement(3093..4649)
                     /locus_tag="IW664_001837"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0849194.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4672..5214)
                     /locus_tag="IW664_001838"
     CDS             complement(4672..5214)
                     /locus_tag="IW664_001838"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0849195.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5201..5725)
                     /gene="atpF"
                     /locus_tag="IW664_001839"
     CDS             complement(5201..5725)
                     /gene="atpF"
                     /locus_tag="IW664_001839"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0849196.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5816..6031)
                     /gene="atpE"
                     /locus_tag="IW664_001840"
     CDS             complement(5816..6031)
                     /gene="atpE"
                     /locus_tag="IW664_001840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0849197.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6084..6803)
                     /gene="atpB"
                     /locus_tag="IW664_001841"
     CDS             complement(6084..6803)
                     /gene="atpB"
                     /locus_tag="IW664_001841"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0849198.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7105..9948)
                     /gene="rnr"
                     /locus_tag="IW664_001842"
                     /pseudo
     CDS             complement(7105..9948)
                     /gene="rnr"
                     /locus_tag="IW664_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(9951..10697)
                     /locus_tag="IW664_001843"
     CDS             complement(9951..10697)
                     /locus_tag="IW664_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0849199.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10809..11045)
                     /gene="secG"
                     /locus_tag="IW664_001844"
     CDS             complement(10809..11045)
                     /gene="secG"
                     /locus_tag="IW664_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0849200.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11163..11441)
                     /locus_tag="IW664_001845"
     CDS             complement(11163..11441)
                     /locus_tag="IW664_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0849201.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11554..12522)
                     /locus_tag="IW664_001846"
     CDS             complement(11554..12522)
                     /locus_tag="IW664_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0849202.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12737..12781
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12805..13779
                     /locus_tag="IW664_001847"
     CDS             12805..13779
                     /locus_tag="IW664_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0849203.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13784..14359
                     /locus_tag="IW664_001848"
     CDS             13784..14359
                     /locus_tag="IW664_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0849204.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14426..15565)
                     /locus_tag="IW664_001849"
     CDS             complement(14426..15565)
                     /locus_tag="IW664_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0849205.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(15965..17263)
                     /gene="eno"
                     /locus_tag="IW664_001850"
     CDS             complement(15965..17263)
                     /gene="eno"
                     /locus_tag="IW664_001850"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0849206.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(17379..18134)
                     /locus_tag="IW664_001851"
     CDS             complement(17379..18134)
                     /locus_tag="IW664_001851"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0849207.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(18238..19428)
                     /locus_tag="IW664_001852"
     CDS             complement(18238..19428)
                     /locus_tag="IW664_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0849208.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
BASE COUNT         5234 a         4172 c         3517 g         6508 t
ORIGIN      
        1 aaaagtcttg tcgcagtccc ttattccaaa taaacggtgt tcaatcatct gaccctcggt
       61 attagctcaa aaaacggcaa aatatgagga actattttcc atttgtttct ctaatactca
      121 ggacaaaacg ctgatttcac aacctccgat atacggtgtt ccagaagtag cttcttcgaa
      181 aaataagctg agatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatgtc
      241 ttgaagctga ccacttctgt cataacctct ttttactgtt cttaaacacg gataaacatc
      301 cgcatctcat tttatgagtg cttagatact ttgatacgat tgattgctcg gtgcaacgca
      361 acagttgccc gtttcaattc gttcgtatct tgttttgctt ttgcttcttc gatttggcgt
      421 tctgcgcgtt gttttgcacg ttccgcacga gatacatcga tatcacgttc acgttccgca
      481 ctatcagcaa cgattgaaac aacattatca cgaacttcca tgattccgcc attaacagcg
      541 atccaatcga cgtgggtatc tgaatctgtt cgttttacac gaacttcatc aatcgtcaat
      601 ggaacgatga ttggtgcatg tttaggcaaa atcccaagct caccagctgt cgtacgtgca
      661 acgacgatag ctgcatggtg atcatagacc aaaccggcag gagttaccac atttactgtt
      721 aaatattgat ccatggggca cccctttcta gtagcctagt gttttcgctt tttctacgac
      781 gtcttcgatt cggccaacac ttcggaaagc ttcttcagga agatcatcgt atttgccttc
      841 caagatttcg cggaatcctt tcactgtttc ttctacaggg acatatgaac ctggaagacc
      901 agtaaattgt tcagccacgt taaagttttg tgacaagaag aattggactc tgcgggcacg
      961 agaaactaac actttttctt gatcggataa ttcatccata cctaagatag caatgatatc
     1021 ttgtaattca cgatatcgtt gcaatacgtg ttggacttct gttgctactt tataatgttc
     1081 ttctcccacg atttcaggtg ctaaagcact tgaagaagaa gccaatggat ctaccgctgg
     1141 ataaatccct tgttctgtta atttacgttc caagttggtt gtcgcatcca aatgggcgaa
     1201 tgctgttgcc ggcgccgggt cagtgtagtc atcggctggc acatagattg cttggatcga
     1261 tgtgatcgaa ccttttttcg tagatgtgat acgttcttgc aattgaccca tttctgttgc
     1321 caatgttggt tgataaccaa ccgcagaagg catccgtcct aacaaggctg atacttcaga
     1381 tccagcttga gtgaaacgga aaatgttgtc gataaacaac agtacatctt gaccttctac
     1441 atcacggaaa tattccgcaa tcgtcaaacc agtcaaagca acacgcatac gtgcacctgg
     1501 tggttcgttc atttgtccga acaccatggc tgttttttcg ataactcctg aatctttcat
     1561 ttcataatac aagtcattac cttcacgagt acgttcacct acaccggtaa atacagaaat
     1621 accaccatgt tcttgggcga tattatggat cagttcttgg atcagtacgg ttttaccaac
     1681 accggcacca ccgaatagtc cgactttccc accttttaaa tatggggcaa gcaaatcgat
     1741 aactttgatc cctgtttcca aaatttctgt acttgtactt aattcgtcaa aggctggtgc
     1801 ttttttatga atttcgcttc tttccgcatc ttctgggaaa ggtgtttcta aatcgatcgt
     1861 atctcctagt acgttgaata cgcgacctaa tgtttcttta cctacaggaa cagagattgc
     1921 tttgcctgta tcaatgactt ccattcctct ttgtaaacca tctgttgatt ccatcgcaat
     1981 ggtccggatc actccgtccc ctaattctaa cgctgcttca agaacaactt ttgatttatt
     2041 ttcgtcattt ttataaacaa ctaatgcgtt gttgatatct ggtaaggatt ggtctaaaga
     2101 aaattccacg tcgacaacgg gaccgattac ttgaacaatc ttgcctgaac tcatcttttt
     2161 tcctccaatc tcaatactat tctagtgccg aagctccggc aacaatttcg gtaatttctt
     2221 gggtaatagc cccttgtctt gcacggttgt aagaaaccgt cagctcatca atgatattag
     2281 cagcattatc agttgctgtt ttcattgctg tcataccagc agcatgttct gctgttttgg
     2341 cgtcaacgat cgcaccgtag atcaaacttt cagcatactg aggcaataac tgagacaaga
     2401 tttcttcttt tgaaggttca aaaatatatt cttgttcgaa agtagttgct tcttctggat
     2461 ctaagtctga gattggcagc attttttcta cacgaaactg actagttagc gaattgatat
     2521 gatggttata acatacgtat aattcatcga atacttcgtt ttggtacata gttgttgcca
     2581 tatttacgat tttgcggact tcatcaaagc ttggctgatc agatagattg cgcagctcat
     2641 atgctagatt gataccgcgt gctttaaaga agtctgcacc agttccgcca attgcgatca
     2701 tcacgtaatc atcgggtgat tggtggtctt cttctaaaat agacatcgtt tgtttcaata
     2761 tcgagctgtt atatccaccg actaaaccgc catctgcagt gatgacgata taacccgttt
     2821 tcttaacagg acggctgagc aacatgctgt tgtaattgat atcccctcta ggattttctg
     2881 aggcaatatc atttaactga gtagcagtca aatgcgtaac gatttctctt acttttgttg
     2941 cataaacctg aaacttctgt gaagaagctt cagattttgt caacttagaa gcagacacca
     3001 tctgcattgc ccgcgtgatt tggctcgttt ttttcgttga agcgatacgt gtcttgattt
     3061 catttaatga agcacccatt catctcacct cactatgcat tttgaatgga tttcaattta
     3121 tcttctgtag aggaattatt tccttttgta gctaagaaca tgtctttata ttcctgaatc
     3181 gcactgttca acgcttcttc ttctggaaga tcttttgttg tacggattgt ttcaaagata
     3241 tctgaatgat tggtatccag atattcgaat aattcatgtt cgaagtctaa aatactgtct
     3301 acagggatac tatctaagaa tccatgtgtc aaagcataca aaatcactac ttgtttttca
     3361 acagctagcg gtgcatgcaa tttttgtttc aagatttcta ctgtacggcg tccacgattt
     3421 agttttgctt gtgttgctgc atccaagtca gaaccgaatt gtgtaaatgc ttctaattca
     3481 cggtaacttg ctaagtctag acgtaaagtc ccagcaactt ttttcattgc tttgatttgc
     3541 gcagaacccc ctacacgaga tacagatagt ccggcatcaa ccgctggacg agtacctgcg
     3601 tagaacaagt cgctttctaa gaagatttgt ccatcagtga tcgaaataac gtttgttggg
     3661 atataagcag agatatctcc agcttgtgtt tcaacaaatg gtaaggcagt cattgaaccg
     3721 ccacctaaat catcacttaa ttttgctgca cgttccaata agcgtgagtg taagtagaaa
     3781 acatcccctg gataagcttc acgacctggt ggacggcgaa gcagcaagga aagttcacga
     3841 taagcaacag cttgttttga aagatcatca aaaatgatca agacatgttt cccgttgtac
     3901 atgaactctt cacccattgc agtaccagca tatggtgcga tataaagcaa cggcgctggt
     3961 tgagacgcac cggcattcac aacgattgtg taatccattg cgccatattt tttcaatgtt
     4021 tcaacttgtg tacgaactgt agaatctttt tgtccgattg ctacatagat acagatcata
     4081 tcttgacctt tttggttgat gatcgtatcg atcgcaatgg aagttttccc tgttttacgg
     4141 tcaccgataa ctaattcacg ttggccgcgt ccgattggta caagagcgtc gattgctttt
     4201 agacctgttt gcattggttc gttaacagat ttacgttgca taacgcctgg tgccattgct
     4261 tctactggac gagctttgtc agtaacgatt tcacctagtc cgtcaattgg ctgacctagc
     4321 ggattaacta cccgtccaat caaggcctct ccaactggaa cttccatgat ctttcctgtt
     4381 cgttttactt tatctccttc acgaatggac tcgaaatcgc caaggataat gatacctaca
     4441 tcatttgatt ctaagttttg cgccattccg tatgagccat ttgaaaattc aagcaactca
     4501 ccactcatcg cgttttctaa accatgtgca cgagcgatcc cgtcacctac ataagttacg
     4561 gtaccgattt cttcgactga aagcacgttt tgataatttt caatttgttc tttaatcaag
     4621 gcactaattt cttctgcttt gatggccatt cgattcacct acctctttgt actatctatt
     4681 taattgatta cgcatatttt ctaattgcgt gcgaatgctt ccgtcgatta ctcgatggtt
     4741 agcttcaaca atcgcgccac caataataga agaatcaacg atttgtttta attcgactga
     4801 ttgatagttc attgtttttg caacattctt ttctaattgt attcgttgtt cgtctgaaag
     4861 tggaactgct gttgtgacac ttcccaacag caaaccttga tgttcatcat atcgacgttc
     4921 atattcttcg atcatgaaca aaagatcatc cattcggtta tacgcataaa caacttctaa
     4981 aaagttttta acgattccat catagccact aacaagcttg tccatgattt ctcgtttttc
     5041 atgaggttct agacgaacat cacttaacat attacctaat ccaggtactt gttcatagat
     5101 ttgacgtaag cttacgagat cttgataaat ttcttctgct ttatttgaat cgatcgccag
     5161 ttcaaataaa gctttcccgt aacgtcttcc tactgtatat ttatctagtt tcatttgaag
     5221 aacctagacc ttcgatatat tgattgatta atgattcgtg catttctgga gataattctt
     5281 tgttcaagat tttttccgcg atttgaagag atagttctgc aacatcgtct tttacagagt
     5341 tcagcgctga atcacgttct acagtgatat cagcttgggc tttacttttt agacgagctg
     5401 cttcttcttg agcatccttc aaaatatttt ggcggcttaa ttctccactt tctttcgcat
     5461 ttttgatgat atcagctgca tcagaacgag aggctaacaa ttgttgttcg cgttcttgtt
     5521 ccattttcgc tgagttgatg cgagattgtt ctgcagaatc caaatcattg gcgatcttgt
     5581 cttcacgttt tttcaaaata tcgctgattg gtccccaagc aaaatgcttc aagagagcca
     5641 gtagtatcaa aaatgagccg ctgacaacga tgacatttcc taacatcgca ttgccaactt
     5701 ctgcaattgc caattgattc agcataggcg agccttcctt tctttgaagt acttctagtt
     5761 ttcatgtaaa aaagcagtaa caaaccaaat tgtttctgcc ttaatactga ttcaattaaa
     5821 caccaaaaac tagaatcaag gcgacaacta cccctaagat aggaacagct tcgactagag
     5881 ctaccccgat aaacattgtt gtacggaatt gaccagacat ttcaggttgg cgagccattg
     5941 attcgattgt tttagagata acttgaccgt taccataacc ggctccgatt gctgctccca
     6001 tgattgcgat tgctgctgcg atataattca taattgtttt cctcctaaat tagataaata
     6061 gtagtttgtt tttaaaacga atattattct tccacttcaa tcttatggct catataaacc
     6121 atagataaag taacgaagac aaatgcttgg atgcttccga taaacaagga gaatgcgatc
     6181 cagaccatct ctaatggaat tgctaacggc aatgagaacc agcctaagct tattaccatt
     6241 tttgcaatca aggttagcaa aacttccccc gcataaatat tcccataaag acgtaaagca
     6301 agtgttaata agtttgtaaa ttcttcaatc agtttcatcg gaaacataaa actatatggt
     6361 ttcaagaacg aatttacgaa atatcctttg aagcctaact ttttcacccc aaataagtga
     6421 gtaagtaaga tcattataaa tgccaaagtt aaagtaacaa acgggtctgc tgtcgggctt
     6481 ttccaaagtg ttgtttcgtt aggtaaaacg atttttgtta ctaaaccgat gttgtttgca
     6541 acaaaaacaa ataaaaacaa tgtaaatgcc aacaaatgga aattgctcaa ttcttttcta
     6601 ggcagattat ctgttacgat tcctcgacta aaatcaatga cccattctat cgcattttgt
     6661 ttgcctgttg gtttcaactt aagatttctt gtaaaaaagt aagctaagaa gaaaacaatc
     6721 aaacaggtca gtaacaccat tatacaaact gtaccatcaa accaaattgg tccaatgtgg
     6781 aacgttagcg agcgttcatc caatccattt cacctctttt ccccgaaatt cacataaaat
     6841 caattcatgt gcgtatctgg ttttttccaa tcggtttcta accacacggt atcataccac
     6901 catgaaacag aaaattcaaa acattttcat gaaaattaga cactttttta tttcagtcaa
     6961 aaacgaagcg aatttttctt tatacacggt gctcctgatc taagaaaaaa cctatgataa
     7021 tcatgtttta tcagattaaa tgctaacaaa ttttatcatt aagtaaaagc aaaagcaccg
     7081 aatatgcgaa aaaggataat gaaattatct agagaaattt tttaatgttc gatcaatttg
     7141 tcgatcgata tcttttcgtt tcaaatcttc ccgtttatca taagattttt tccctttcgc
     7201 cagaccaatc aataccttgg catagccgtt acgaatatag acttttaaag gaacaacagt
     7261 gattccagta tttttcaatt cattttcaag ccggatgatt tgttttttgt gtaataataa
     7321 ctttctcgtg cgcaacggat catgattaaa aatatttcct tgttcataag gactgatatg
     7381 aacattatgc aagaaagctt ctccgttgcg gacgcgaata aatccatctt ttagattgat
     7441 ccggctgttt cgtatcgact tgatctcggt tccttgcaag accatccctg cttccatcgt
     7501 gtcgatgatc gaataatcat ggcgagcttt cttgttttgt gcaattaatt ttccctcgcc
     7561 ttttggcata gctgtttcct ccaactcatt ttattttttc tttttcttct ttttaacacc
     7621 tttgtaaaat ggcttcttcc cttttttctt accgaacgtt tgcttactat cgctatatcg
     7681 tttgttcttc ttgccatttc tttctgggcg ttttcctgct tgttttcctt cgcgttttcc
     7741 tgattttcca tttcgtgttt ttggccgttc caacgctgta acctcttctg cggaaacaag
     7801 ttcaaaatct actgctcgtt catctggatc agctttgact acttttactc gcactttctg
     7861 tccgatttta aaagtcagac cagttctttc acctacaaga gctaaatgat tttcaacaaa
     7921 atggaagtag tcctgtttca attcattgat atggatcaat ccctctactg tattcggcag
     7981 ttcgataaac aggccgaatt tcacaacaga gctgatgatc ccatcgaatt cttcccccac
     8041 tttgtccatc atgaattctg ctttcttcat ggaatctact tcacgttctg tttctactgc
     8101 acgacgttcc atcttagaac tatgatctgc aatttcagga agttccgtag cccacgaact
     8161 ttgattgctt tcacttttat cttcactgta agaacggatc agacgatgga cgattagatc
     8221 gggatagcgt cgaataggag atgtaaagtg tgtgtagtaa tctgccgcta agccataatg
     8281 cccaaaattg tcttctgaat agcgtgcttg ttgcatgctt cttagcaaca tagtattgat
     8341 aactgcagct tctggtttgt cttctactcc ttcgatcaca tgctgtaaat cttttggtgt
     8401 gatcgttcct ttcgttcctc ggaccagaat gcccaaagct gaagcaaaat caaagaagcg
     8461 ctgcattttt tcttcttttg gctgttcatg gattcggtaa ataaacggca gatttaattt
     8521 attataatgt tcagctaccg tttcattggc cgcaagcatg aaagattcga tcaagcgttc
     8581 accaattcct ctgcttctta gctggatatc atttgggtgt ccttcagcat caacaaggat
     8641 ttttgcttcg cgatcctcaa aagaaatcgc gccacgtttg attcgcatcg tttcaaggat
     8701 tcgatgcaat tcacccattt cttggaacat aggtacgagc tgagcatagc gttgcatcgt
     8761 ttcttcgtct tgatcttcta agatttgatt gacagctgtg taggtcatcc gttctgttgt
     8821 ttggatcaca cttgggaaaa tatcatgaga gactacttgt ccatttggat caatctccat
     8881 ttcgcaactc atggtcagcc ttggaacatg gggattcagt gaacagatac cattcgataa
     8941 acgttgcggg atcatcggaa tcactcgatc agtcaaatat acacttgtcc ctctttcata
     9001 tgcttctctg tccaattgac tgccttgtgt cacatagtaa gaaacatctg caatgtgtac
     9061 tccaagaaaa taattaccgt tatctaattt tttaaccgta actgcatcat ctagatcttt
     9121 tgcatcttct ccatcaatcg taacaatgag ctgatttctt aaatctctac gtccagttat
     9181 atccttttcg ctgatttgat ctggaacttc atctgcttca gacaatacat catcaggaaa
     9241 tttcgtaggg ataccattag ccacgacaat tgagaggata tccatgccag ggtcattctt
     9301 atgaccaatc acttgcttga ttatcccttc taaactagtc gcataccctt tttcagggta
     9361 atgggtcagt tcgatcagca caatacttcc gtcaactgga cgaactccct ctgctgctgc
     9421 atatacttta aattggttga gctttctatc tttcgggata acaacgccgt aaagatccgt
     9481 ttcttgtatt tcttcttctg aataagcaac gaattcccct gctaactgag tagtcgcccg
     9541 gtctcgaatc tcgactactt tcccctctgc tccgcgatcc atatctgtat ttgctcgctt
     9601 catgatatcg attgctacgg tgtctccatc cattgcaaaa ccagtcgctt cttttgggat
     9661 atagatgtca tcttcttcgg gatcaatcgt tacaaatcca aatcctcgtt catttgctcg
     9721 aaatgttcct tcgatcaaga ttggttttaa aggaagtttc acttttcctt ttttattgaa
     9781 aactatactc tgttctcttt ccatttgagc aatcgtttgt actagtaatt taaaatcatc
     9841 gcttttttgg aaccctaaac cttcagcgat ttcttccata gaaaaactgc gtttgcgact
     9901 gctttccatg aaaaagagaa ttttctcttt tatcgtttct tttgtcatat ttattcctca
     9961 ttccaaggta atttttctaa aaattccaag acgtcttttt ctaattcgcg tcttgctgta
    10021 ccaactgtaa tcacatgacc actttcagga taccatcgaa gtgtcactcg ctgacggctc
    10081 aacttgcgtg cagtttcaaa tacgccatta gggtcgatca tttcatcttt cccagcttga
    10141 gctaaaaata tcggctgttg gatatctgac aacctacttt ctacgattgc tgcctgatct
    10201 tgtatatcca ttaattgttg ttcagctaac gagcggtaag caactgcttt ttcattgatt
    10261 tctttctctg atttacctgc tgtcttcagc acttgacgta cgtatttttc aaagttttca
    10321 ggcacgtttg tttttacagg agacaaaggc gaacagaaaa aaccgccgcc aatcactgat
    10381 tcttcttcta gtgctcgtac agcaaagatg ccgcccattg ataatcctaa tacggcaata
    10441 tcagaaaact gctcagactg taaaaaatgg atggcttttt tagtgtctgc ccaccagctt
    10501 tctgcttttt cttgcaaaat atcatatggc tccattgttc cgtgtccttt aaaaagcggc
    10561 gcataaactg tataatctgc tttttccaaa aatcgtgcga gcattcgaac atcgtttgga
    10621 cttcctgagt aagcgtgaag cagcaaaacc gctcgctttc catgttttgc ataaaggggt
    10681 tttggtaatt gtctcactcg tctcacctct ttcgcctcat tttaccatag tttctttcat
    10741 aacttcttct taagcgttct ctttgcaata aaaaaagccg cccgccgttt tgcgagaggc
    10801 ttattcaact acttcgaaga taaatatgct aggacaaata ataagatcat ccagatagct
    10861 cccataactg cagttgatcg ctgcatgaca gcttcaaagc cgcgagcttt ttgtttgccg
    10921 aatagttgat ccgcaccgcc ggtaaatgca ctcgctgcac tattttgctt gcttggctgc
    10981 atcataatcg caatgaccat aacaatcgat aaaacgatca cgattcccaa aattaaatta
    11041 tacatatcgc agcctccttc ccaaaagata tcgtctcttt aactttatca tagataaaag
    11101 tccgtgacaa ctctatataa atggaataac agctgaaagt tcaaaacttt cagctgttat
    11161 tcttatgact gacgtaagac gtcttgatat tctggtttct tttcaaactc tttttttgca
    11221 taagggcata aaggaatgat ctttttcccg tctcgtcttg ctaattctac tccagtatac
    11281 actagctttt cagctaactt ttgaccacga tatgctggat ctacaaatgt atggtcaatg
    11341 atcatgatgt ctgggcccgc atctgaccat gtcatttctc cgatctcttg ttgttcatca
    11401 ttgaataaag caagacgatt tttttcttct ttgatttcca tatgccgcac tcctcaaata
    11461 actgatattt ttttattcct accttatttt agcagtctcc tgatctataa acaattattt
    11521 atcctcgaat tacttacaaa aaagaagaga aaattaattc atcgttattc tttgttcaat
    11581 aaatgaaaaa atcactgtcc aataggtttt cggatctttc atcgatgctt ccaagtgttc
    11641 tgcttctgga acaagcagca gtgctttatc ccctgccgta gcattttgaa tcgtgtacgc
    11701 tgcttctgta gggacaaaat gatctctttc accatgaatg accaataaag gcagatggtt
    11761 gctgccaagc tgtctcgtag ctgatgcttg ctttaaggag taaccggcgt atttctgggc
    11821 ataatgattc gcatatctca tgaccatttt tttaggaaag acagtgattt tacttaacat
    11881 atatcggaat tctgcataaa cagaaacata tccgctgtct aaaataaatc ctttgaccgc
    11941 tgatggaagt ttttcaccac ttgccatcat gatcgtagaa gctcccattg atccgccgtg
    12001 taaaataatg gacgcattcg gctgttcatc taagatgagt tggatccagg caattaaatc
    12061 tagccgatct agccatccca ttccgatgat ttctccttca ctttctccgt gcgcacgtaa
    12121 atcaggtaca agaacgttga atcctttctc tgcatatctt ttacctatat gagacatgtc
    12181 tcttttccct gtgcttcggt aatcatgtac gcaaatgacc catttattct gcttatttgg
    12241 atggatgaac atctgaccag ctagtttcaa cccgtcataa cttttgattg ttttgggtgt
    12301 tccctgctcc caaaagcttt cacactcttc ttctaatttt tgtcgttttt tttctactga
    12361 cgtgactgca gcagggtcca tcaaacgatg accagtgtct tcataccagt catatcctct
    12421 ctgtactccc gctcttacta atttctttgc ataaaaataa gaaagaaaat ggtaactgat
    12481 ccctataatt atcagaataa gaatgaaata aaaaataacc attgtacacc cgccgctttc
    12541 tgccaaacta taatctaact attatccaat gtaccgatcc ataggattct ttattttcgg
    12601 cacacgtaaa ttccatttct ttttcattgt atcatttact taaagttctc ctcaatagat
    12661 ttacttgtaa gagagcattt acattcgcaa taaaagccag tatactaatt tcgtgcatta
    12721 taatcaagac gctcgtcttg gactggttcg gaaacttccc agaataaaaa actaagtatc
    12781 tctaaagaaa ggagattgga aataatgcga aaagtaatta ttgattgcga tccagggatc
    12841 gacgacactt tagctttatc attagctgtc aaatcccctg atatcgaagt agttgctatt
    12901 accgtagttt gtgggaatgt ccctgcagat ataggtacgc agaatgtgct gaaatgcctg
    12961 gaacgctgtg atcgtttaga cattcctgtt tatcaaggaa tggaaaaacc tttgaagcaa
    13021 cctttcatca gtgcacaaga cactcatggt atggatggtt taggggaaac aaatttcccg
    13081 atgatcttga gaaaacaggc agaacctctc catgccgttg attttttggc tgattatttc
    13141 aaagaaaaga caaatacatc agtcattgct ttgggtcctt tgaccaatat cgcttttgct
    13201 ttgaaagtca acccaaatat tgggaaacat atggatcgtt ttgtctcgat gggtggaact
    13261 tacaaaagcc atggtaactg ttcgccagtt gctgaataca attactggtg tgatcctgaa
    13321 gcagcttctt atgtattcga gaatctcaaa caaacgatag aaatggttgg gttagatgtg
    13381 acaagagaaa tcgtcttgac accgactatc ctcgaatact gctgtcaaat gaatcctgaa
    13441 gagggagagt atctaaaagc gatcactcgt ttttattttg acttccactg gaaacaagaa
    13501 agaattttag gatgtgtcat caatgatccg ttagctgttg cttattttat cgagggggcc
    13561 atctgtgaag gtttcaaaag cttcactgct gtggaaactc aaggaataag tcgtggacaa
    13621 acattggttg atcgttatga attttggcag aaacctgcta acagtaaaat tatgacaaaa
    13681 gtagatactc gtcttttttt ccgaaagttt ttaaccgttc ttttaaatgc acaagaagaa
    13741 acgattatga aagatttgga aaggttaaag atgggctaac aaaatgaata aaaacaaaat
    13801 ggcaactcgc gtacttacag tgatggcttt gtgtattggg atcaattata ttggaggaac
    13861 gattgcttta tggcttcgtt tgccaatcta tctcgattct atcggaacga tttttgctgg
    13921 tgcattgctt gggcctgtac ctggaatgct gacaggattg tccagcggta ttttaagtgg
    13981 agtgactaca gatatttttt ctctgtatta ttcgccaatc caaattctca caggcttgct
    14041 tgctggattg attttgccaa gatatctcgg caaagataaa aacagcttga aactgttatt
    14101 actcgctttg attttgtcgg tacctggaac gatcgtttct tcgatcatta ccgttcagtt
    14161 atttggcggg atcacctcct caggatccag tatgatcgtt cagctattac atgggttagg
    14221 tttaagtcag acaacaagtg tgatcttggt acaggcaggt acagactacc ttgatcgttt
    14281 gttgtctatc gtcgttgttt cattagtcat cactatgttg ccacaacgct tgatatattc
    14341 aaaaagaaaa ataggctgaa taaacagagc tggattagca aaataatgct aatctggctc
    14401 tgttttttca tttattaaaa aaatcttacg ctaagtagga taaaataatt ccttgatatt
    14461 tttcaatcat atccaaataa ttatcgattt caacatactc atctacttga tgaggcaaag
    14521 taacgacacc aggcccaaag acgacaaagt cgaaggaatg gttagctttc gtaaattcgg
    14581 ctgcatccgt tgttgctgcc gctccaacta aaggtaatgg ctggctgaat tgttgctgaa
    14641 tgcaatgaat caaaggagaa tctggatcag ctttgacagg tattttgttg taatcgatca
    14701 tcaattccaa atgatagtct gtctcttgat tcaattcatt gacgattgat tgcagcaatg
    14761 caatgatttt atcatttggg tattctggaa tcgatcgaat gttcccttgg agctgcgcat
    14821 ggcttgggat gctgttgact tgattcccgc cactgatcaa tgtaacatta tggattgttt
    14881 ttcctaggac aggattctcg attgtttcag cgaggtgatt catttcagca tttgctttcg
    14941 taatgaattc atttaggtga ttgattgcat tataaccttg gtctggcata gagctatgcg
    15001 cttcttttcc gtgcgaggtg actgtatagt tgattgatcc catgtgcgta tacatcaaac
    15061 tgtaattagt tggctctccg atgatcaagg catctaagtc atctacatag cctgctttcg
    15121 tgagctgttc cccacctagt tcacccactt cttctccgac agtggctaat aatttgactg
    15181 ttccgttaaa tggttttcct gactctttca gttcgatcat ggcaatcacc attgctgcca
    15241 agccactctt catatctgta gcaccacgtc cgtacaaacg gttcccatgg atttctgctg
    15301 caaagggtgc ataggtccaa gacgattcat caccagccgc cacgacatcc atatgcccag
    15361 ataggcctaa cacttttcct gattggccct tttggtaacg agcaatcaaa ttatctctac
    15421 catctctgta agaaacaatc tctcctgtta tgtcatgctt tgccaaaagc ttattcaaat
    15481 aggctgctac ttcaccttcg tttccattca ctgatttaat tcggatgatc tcttgtaaaa
    15541 tcgcgatttt ttcttctttt ttcatctaat cctctccctt gccatttctt tttattgtat
    15601 ttgagtgtgc tcgaattttc aaataggagc aaccaaagta aaaatgaaga aagagagcca
    15661 ctttatgaaa aatatgctgc tacttccctc ctattttatt tatacgagag gacttgctca
    15721 tagattccta ttctactgtt ctatccttaa aagctcttat atgatgctag ttattgttag
    15781 ctttcctttt tcaaggatga tcttttcttg taccattctt tgtaaacatc atatacagaa
    15841 gctagttgca aaaaaaactc tctacctgct gatatttcag caagtagaga gttcgattac
    15901 ttaactaagt gatcatactc gactatcttt caatagtcga tcatgtatct tcattagaaa
    15961 agaattattt gttttttaag ttgtagaaag atttcaaacc tttgtattct gcaacttcac
    16021 caagttggtc ttcgatacgt agtaattggt tgtatttagc aatacggtca gtacgtgata
    16081 gagaaccagt tttgatttgg ccagcgtttg ttgcaacagc gatatcagag attgttgaat
    16141 cttctgtttc accagaacgg tgagatacaa ctgctgtgta gccagcttct ttagccattt
    16201 cgatagcttc gaaagtttct gttaatgtac cgatttggtt cactttgata aggattgagt
    16261 tagcaattcc tttttcgata ccttcagata atttttgagt gtttgttacg aacaagtcgt
    16321 caccaactaa ttgaactttg tcgcctaata cgtcagttaa tttcttgaat ccgtcccagt
    16381 cgttttcgtc taatccgtct tcgattgaga tgattggata tttagaaact aattcttcgt
    16441 agaatttgat catttcgtca gttgtttttt cgccttcgcc tgaatcagct agtacgtaaa
    16501 cacctttttc tttgtcgtag aattctgaag aagcagcatc catagcaaga acaacgtctt
    16561 taccaggtac atagccagct ttttcgattg cttcgatgat tacttcaaaa ccttcttcgt
    16621 ttgaaccaag gtttggagca aatccacctt cgtcaccaac agaagttgct aatccgcgag
    16681 ctttcaagat tgaagctaat gcgtggaata cttcagcacc catacgtaga gcttctttga
    16741 atgttggagc gccaacaggc atgatcatga attcttggaa gtcgattgag ttatcagcat
    16801 gtgatccgcc gttgatgatg ttcatcattg gtgttggcaa tacttttgtg ttgaatccgc
    16861 ctaggtagtg gtataaaggt acttctaggt agtcagcagc tgcacgtgca acagcgatag
    16921 aaacacctaa aatagcgttc gcacctaatt tacctttgtt aggagttccg tctaaagcga
    16981 tcatagcttt gtcgatagcc atttggtcac gaacatcgta gccaatgatt gcttcagcaa
    17041 tgatgttgtt tacgttgtcg acagctttag ttacaccttt accgccataa cgagctttgt
    17101 ctccgtcacg taattcaact gcttcgtatt caccagttga agcacctgaa ggaaccattc
    17161 cgcggccaaa agctccgctt tctgtgtata cttctacttc gattgttggg ttaccacgtg
    17221 agtctaagat ctcgcgagcg taaacatcag taataattga catgttttgt ctctcctttg
    17281 agtttgtttt ttcctaaggg atttccctca tatacgattt tagttaattt tgctcattag
    17341 tgcaaacatt tcttgcattt ttcgcaaatt ttcaacaatt attttacagc gtctaacaat
    17401 gacaagaatg aatctgcttg caagcttgcg ccaccgacta aagcaccgtc aacattttct
    17461 tttgccatgt attcagcaat attttctggt tttacagaac cgccgtattg gatacgaact
    17521 gcttctgata cttcttttcc ataaagtttt tcaactgttt gacgtacaac tccacaaatt
    17581 tcgtcagcga tttgtgcgtc agctgattta ccagtaccga ttgcccagat tggttcataa
    17641 gcaataacca tgtttgacac ttgttcagct gaaagatcag ctaaaccagc tgtaatttgt
    17701 ccttcgatcc attcagcagt tttgcctgct tcataggttt ctaaagattc gccacagcat
    17761 aggattggtg tcataccatt tgcaaagata gctttcgctt ttttattgat gtcttcgtct
    17821 gtttcatgga aatattcacg acgttctgaa tgtccgatga tcacataatg aacacctaga
    17881 tcagcaagag ctgctggtga agtttcacca gtgaacgcac ctgagttttc ccagtagcag
    17941 ttttgtgctg agatttttag ttctgtacct tcagctgatg tcattaattc ttgtaagaat
    18001 aatgcaggag atccgatgac tgaatcaact ttgtcatttg aagggatttt gttttttact
    18061 gcttcagcaa attcttttgc ttctgatgca gttttgttca ttttccagtt acctgcgata
    18121 ataggtttac gcattgagga acacactcct taatttattg aaaaatggct agggagtcat
    18181 gaaaactccc ttcacctttt gatcgattac tcgtcagaat tgagcaaact tggtcgttta
    18241 tttatcgttg attgctgcta gtcctggtaa ttctttacct tcaagcaatt ccaagcttgc
    18301 gccgccacct gttgagatgt gtgtgaattt gtcagcaaat cctaattgtt ctgctgctgc
    18361 ggctgagtct ccgccaccga tgattgttgt tgcgccttct aagttagcga ttgcttcaca
    18421 aacaccgatt gtacctttag caaagttact catttcaaat acacccatag gtccgttcca
    18481 aacaactgtt tttgcacctt gtaatgtatc agcaaataat tttactgatg ctgggccgat
    18541 gtctaaagcc atcaaaccat caggaatgtc tccttcgatt acttgtgttt caacatcatt
    18601 tgaaaattct ggtgcacaga cattgtcgat tggcaagaca agtttgtcgc ctgctttttc
    18661 gattaattct tttgctagtt ccactttatc agcttctact agagagttac cgatcttgat
    18721 tccttttgct tcgtagaatg tgtaagtcat tccgccaccg atcaagattt tatctgcttt
    18781 aggaatcaag ttttcgatta cgccgatttt atcagaaact tttgcgccac caaggattgc
    18841 aatcattggt cgttttggtt cttctactgc ttcaccgatg aacttgattt ctttttccat
    18901 caagaatcca gcaactgttg ggataccagt tgaagcgata cctacgttag aagcatgtgc
    18961 acggtgagct gtaccgaaag catcgttaac aaatacatcg cctaatgaag cccagtattt
    19021 acctagttct gtatcatttt tgctttcttt tttaccgtca atatcttcaa atcgtgtatt
    19081 ttcgaatacg acaacgtcgc cgtcattcat gttgcggatt gcttcttcta attgttctcc
    19141 gcgagtttca ggtacgaatg ttacttcttt acctaataat tcgcctagac gttttgcaac
    19201 tggagctaaa gattttcctt ctttatcttc ttctgttttt acacgtccaa ggtgagagaa
    19261 aaggattgct tttccgcctt gttctagaac gtagttgatt gttggcaatg ctgctttgat
    19321 acgagtgtcg tcagtgatca cgccgtcttt caaaggaacg ttgaagtcaa cacggacaag
    19381 aacttttttg tcttttaaat cgatatcttt cactgttttc ttagccattt t
//
LOCUS       DACEKG010000054        18741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEKG010000054 DACEKG010000000
VERSION     DACEKG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.056"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            123..770
                     /locus_tag="IW664_001853"
     CDS             123..770
                     /locus_tag="IW664_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0849209.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1163..3808
                     /locus_tag="IW664_001854"
     CDS             1163..3808
                     /locus_tag="IW664_001854"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0849210.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4118..5431
                     /locus_tag="IW664_001855"
     CDS             4118..5431
                     /locus_tag="IW664_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0849211.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5421..6074
                     /locus_tag="IW664_001856"
     CDS             5421..6074
                     /locus_tag="IW664_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0849212.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6129..6800
                     /gene="radC"
                     /locus_tag="IW664_001857"
     CDS             6129..6800
                     /gene="radC"
                     /locus_tag="IW664_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ0849213.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6695..8047)
                     /locus_tag="IW664_001858"
     CDS             complement(6695..8047)
                     /locus_tag="IW664_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0849214.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8113..9402)
                     /locus_tag="IW664_001859"
     CDS             complement(8113..9402)
                     /locus_tag="IW664_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0849215.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9489..10193)
                     /locus_tag="IW664_001860"
     CDS             complement(9489..10193)
                     /locus_tag="IW664_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ0849216.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10393..10659
                     /locus_tag="IW664_001861"
     CDS             10393..10659
                     /locus_tag="IW664_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0849217.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10827..11057)
                     /locus_tag="IW664_001862"
     CDS             complement(10827..11057)
                     /locus_tag="IW664_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0849218.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11129..11284
                     /locus_tag="IW664_001863"
     CDS             11129..11284
                     /locus_tag="IW664_001863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849219.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11593..11817)
                     /locus_tag="IW664_001864"
     CDS             complement(11593..11817)
                     /locus_tag="IW664_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0849220.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12084..12419
                     /locus_tag="IW664_001865"
     CDS             12084..12419
                     /locus_tag="IW664_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849221.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12652..13593
                     /locus_tag="IW664_001866"
     CDS             12652..13593
                     /locus_tag="IW664_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0849222.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13595..14485
                     /locus_tag="IW664_001867"
     CDS             13595..14485
                     /locus_tag="IW664_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ0849223.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14528..15328
                     /locus_tag="IW664_001868"
     CDS             14528..15328
                     /locus_tag="IW664_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0849224.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15343..16194
                     /locus_tag="IW664_001869"
     CDS             15343..16194
                     /locus_tag="IW664_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0849225.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16191..16550
                     /locus_tag="IW664_001870"
     CDS             16191..16550
                     /locus_tag="IW664_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0849226.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16565..16726
                     /locus_tag="IW664_001871"
     CDS             16565..16726
                     /locus_tag="IW664_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0849227.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16723..17049
                     /locus_tag="IW664_001872"
     CDS             16723..17049
                     /locus_tag="IW664_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849228.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17046..17861
                     /locus_tag="IW664_001873"
     CDS             17046..17861
                     /locus_tag="IW664_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ0849229.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            18000..18311
                     /locus_tag="IW664_001874"
     CDS             18000..18311
                     /locus_tag="IW664_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849230.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18308..18538
                     /locus_tag="IW664_001875"
     CDS             18308..18538
                     /locus_tag="IW664_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849231.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            18545..>18741
                     /locus_tag="IW664_001876"
     CDS             18545..>18741
                     /locus_tag="IW664_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ0849232.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEE"
BASE COUNT         6318 a         3102 c         3805 g         5516 t
ORIGIN      
        1 atgcaagctt ttgaacaccg ttttatttgt ctcacaccct tttccttcct acttgtaacc
       61 ttgctcccgt gttagaaaca tgttacacta aatatgtgaa attattaaca aggagcgtcg
      121 ttatgcgaga aaaaacagat acactgccaa aagcaactgc gaaaagatta cctttatatt
      181 tacgctactt gaagatgctg gatgactcag gtataagtag aatcaaatca aatgagttca
      241 gcgaaatcac ccaaattcct tctgcaacga tccgcagaga cttctcgcaa ttaggcgagc
      301 taggcagaag cggatatggc tatgatgtcc cttttcttat cgatgtattc aataatatcc
      361 ttaatactaa agaagaaaaa cgaatcgcat tagtaggata tggaaatctt ggcaaagcat
      421 tgaaacacaa caattttaga agaaatgaaa atctaaatat cgtgtgcgtc tttgataatg
      481 atccagcact gatcaatcga gtaattgatg gagaaatgat ctatccgatt gatcgtttcg
      541 cggagatagc caaagcaaaa aatgtgacag tagcaatctc tacggtacca agtaaatatt
      601 cacaatctgc tattgatgaa attgtcaaag gaaatgtaac cgctatactt aattttgcgc
      661 ctgacagagt aactgtccca gcttatgtca atgttcaata cattgatttg acaaccgagt
      721 tgcagacgct tatttacttt gatgagaatt actcagaagt tttttcttag gaaaatttga
      781 gtaggcttat tgatcttgcg agaactcgtc gtaattcttt caaaaatttt ttagtactaa
      841 atgaagtggt tttttcacga ccacttgttg actaaaaaaa aaaaccattg tagaatgaag
      901 cataacgctg aacaagagga gtaactggca cattcattct tagagagaga actatctgct
      961 gaaaggttct caatgacaac cagtgaaggt agcttgggag tttctgtctt gaatggagta
     1021 gagatagacg agtcgtgatc gttaactcac ttgagaggca ataatgattg attattgcaa
     1081 tttaggtggt accgcgtaaa tcatacgtcc tagaatacga aagtatttta ggacgttttt
     1141 tagtttaaag gaggaaaaca gcatgacgga agaaaataac ctatcaacaa aatataatcc
     1201 gcaagaagtt gaagctggac gttatcagca ttggttagaa caagatttgt ttaaaccgag
     1261 cggagataaa aaagcaaaac cttattcaat cgtaatcccg cccccaaatg ttactggaaa
     1321 acttcattta gggcatgctt gggatacgac cttgcaagat atgctgattc gtcaaaagag
     1381 aatgcaagga tatgatactt tatggctgcc tggtatggac catgcaggaa tcgcgacaca
     1441 agcaaaagta gaagcgaagc ttagagagca agggatttct cgttatgatc taggtcgtga
     1501 aaagttcatc gatcaagtgt gggaatggaa agaagagtat gcgagccaca tccgcgaaca
     1561 gtgggctaaa atgggcttat ctttagatta cagtagggaa cgttttacat tagatgatgg
     1621 attatctgaa gcagtacgta aagtttttgt taccttatac gaaaaaggtt tgatttatcg
     1681 tggtgaatat atcatcaact gggatcctca agcacgaaca gctctatctg atatcgaagt
     1741 tattcataaa gatatcgaag gcgcctttta tcatatgagc tatgaactag cagatggatc
     1801 aggcgtagta gaaattgcga caacccgtcc agaaacgatg ttgggagata cggcgattgc
     1861 tgttcatcca gaagacgagc gttatcaagc gctcatcggc aaaaaagtga ttctaccttt
     1921 agtcaataaa caaatcccaa tcattgcaga tgaatatgta gatatggaat tcgggacagg
     1981 tgtagtaaaa atcactccag cacatgatcc aaacgacttt gaggtaggta atcgtcatga
     2041 tcttccacgc gtaaatgtta tggatgatga tggtacgatg aatgaactag caggaaaata
     2101 tgctggaatg gaacgttttg ctgctcgtaa agcaatcgtg caagatctga aagaaatcgg
     2161 tcgattgatt aaaatcgata cgatgatcca cagtgtgggt cattccgagc ggacaggtgt
     2221 cgttgttgaa cctcggctgt ctactcaatg gtttgtaaaa atggcgccgt tagcgaaaaa
     2281 agccattgat aaccaagaaa cagatcaagc agtggaattc ttccctccaa gattcaatca
     2341 aacgttttta cgttggatgg aaaatgtcca tgattgggta atctcacgac aattatggtg
     2401 gggacatcaa attcctgcat ggtatcacaa agaaacaggt gaaatgtatg tagggatgga
     2461 agcaccagcg gatagtgaaa actgggtaca agatcctgat gttttagaca cttggtttag
     2521 ttcagctttg tggcctttct caacaatggg ctggccagat acggaaagcg aagattataa
     2581 gcgatacttc cctactagca ctttagtaac cggttatgac atcattttct tctgggtaag
     2641 cagaatgatt ttccaaagct tggaatttac tggcgaacgt ccatttgaga atgtcttgat
     2701 tcatggattg attcgtgacg aacaagggcg gaagatgagt aaatcattag gtaatggaat
     2761 cgatccgatg gaagtaatcg aaaagtatgg agcagatgct ctccgctggt tcttatcgaa
     2821 tggttctgca ccaggacaag atgtccgctt cagctatgaa aaaatggatg cttcttggaa
     2881 ctttatcaac aaaatctgga atgcttcacg tttcgttatc atgaatatcg aaggaatgac
     2941 cgtagaagag atcgatttat ctggagaaaa atcagtggct gaccggtgga tccttactcg
     3001 tttgaatgaa acgatcgaac gagtaactga gctatttgat cgttttgaat ttggtgaagc
     3061 aggtcgacaa ctgtataact ttatctggga tgatttttgt gactggtaca tcgaaatgag
     3121 taaagaagta ctttatggag atgatgctgt ttcaaaacaa accacccgta gcatcttagt
     3181 ccatacactt gatcagatcc tacgtttatt gcatccaatc atgccattcg ttacagaaga
     3241 aatatgggaa catattcctc atcagggaaa ttctctagta gtggcagaat atcctgttgt
     3301 acgtccagag tttgatgatg aaacagcttc taaagggatg gaagtattga aagaattgat
     3361 tcgagccgtt cgaaatattc gttcagaagt gaatacacca ttgtctaaac caattacgtt
     3421 gctaattaaa acaaacgatg ctaaaatcga tcaattttta gttgaaaata caagctatat
     3481 cgaacgcttc tgtaatcccg aagagttgac gatatcaagt gagatcactg cgccagatct
     3541 agcgatgtca gctgttctga caggtgcaga aatcttcttg ccgttagcag gattgatcaa
     3601 tattgaagaa gaaatcaaac gattagaaaa agaactggaa aagtggacaa gcgaagtaaa
     3661 acgtgtccaa ggaaaacttt ctaatgaacg ttttgtttct aacgcgccgg aagaagtagt
     3721 agaggcagag cgtgcaaaag aaaaagatta tttagagaag caaacggctg tcaaagaacg
     3781 aatcgaaagt ttgcgtacta ttcaataaga atagaaaaag agagtgtgaa atcagctagc
     3841 tattactaaa agctagctga ttgaacatcg ttacaggaaa gagtttgagc ctcactctta
     3901 gcttcgattc ataaggagat gtgacataaa ttttgtcgca tctctttttt agtctactct
     3961 tattatgtag gagaaggagg gaggtggatc atgtatcaag agtcaataaa gtagttgata
     4021 aaatcgttcc tctctctgtt tcttttctct ttgtcatact tttatttttc ttatgtgttc
     4081 tatttgttat aataaaagag aaatggggga attgttgatg acgattgaag aagcaataga
     4141 atggatccat agtcgattac catttggttc acgtcctggc cttgaccgag tcgaagcttt
     4201 attagaagtg gtagggcatc cagagagaaa agtaaaaacg atccatatcg ctggaacaaa
     4261 cggaaaaggt tctactgtag cttatttgag aagcctatta gaagaggctg ggctgacagt
     4321 aggtacattt acttcacctt atatcgaaac gttcaatgaa cggatcgcaa tcaatggcaa
     4381 aagcattcca gacaatgatt tgatccagct tgtaaaaaaa tatcagccac tgatcaaaca
     4441 acttgatcaa tttgaagcag tagctggtat tactgaattc gagacattaa cagcaatggc
     4501 atttgattat tttttgatga agaaagtgga cgtagcgatt atcgaagttg gcttaggcgg
     4561 cttattggat tctaccaatg tagctgtacc aatgctgacg ggaataacaa cgattggact
     4621 tgatcatacc gaaatacttg gagatacaat tgaagaaatc gctgcacaaa aagctggaat
     4681 catcaaacaa ggcgttccag tagtaacagg aaatattgcc ccagaagctt ttactgtcat
     4741 ccaacaaaca gctaaagcaa aaaatgcacc gctttatgta tacggggaaa attataaaat
     4801 aaactatctg catcctgatg agatgtgggg agaagtattt gaatttttta atgaaatagg
     4861 aaagatccct caattaacca ctccgctttt agggcaacat caagctgaaa atgcaggagt
     4921 agccatcgaa ttgtattttg tttactgtca attggaaaag ctgcctttcc gagaaaaaga
     4981 tgttttgaag ggattgaaga aatctcgatg gccaggaaga atggagcgtt tgagtaacga
     5041 gccattagtt gttttagatg gtgcccataa tacgcatgca atgaaccggc ttgttgagaa
     5101 tttgaaaaaa gaattcaaag gatatcaaat caacattttg ttttccgcct tacgaactaa
     5161 aaatgttaga gaaatgctag aacgattgca gcaagtacca aatgtccata tttatttgac
     5221 gacctttgaa catccaaaag ccatcgaatt aagtgaagta tcagatttag caagtgagag
     5281 agtatcaatc gtttcattgt ggcaattcgg attagcagat atattggaaa agatgacaaa
     5341 tgaagacgta ttagttgtga ccggttcact ttatttcatt tcagaagttc gccatttatt
     5401 aatacaatta ggaggagatc atgaagtata atgcagtgat ttttgacatg gatggtttgc
     5461 tgttcgacac agagttagtg tattacgaag ccacacaagt agtagcagac aagatgggtt
     5521 ttccttatga taaagacctg tatttaaaat tcttaggtgt ttcagatgaa gaagtatggg
     5581 cgaattatca tatgattttc tcggaatttg gcaaaacaag tgtacaaaga ttcatagatg
     5641 attcatacca ggaaacgctt gaacgttttt cgcaaggagc ggtacaaatg aagcccggtg
     5701 tgttggaact cctctctttt ttagaagaac atcagattcc caaagttgtc gcttccagta
     5761 accaacgagc agtcattgaa cttttattga agaagaacaa tctgtttgaa cgatttgaga
     5821 cgattgtttc tgcagaaaat gttcaacgag caaaaccaga cccagaaatt ttcttgttag
     5881 ctaatcagaa gctaggtaca gcgaagaaag aaacattgat tttagaagat tcacaaaatg
     5941 gtattttagc agcttctgcg gctgagatcc cggtagtcat ggtaccagat ctgcttccac
     6001 catcccaaga attgatcaaa aaaacagctg gggttgtttc ttctttacat gaaatccctg
     6061 cattgttaaa ataaaaatcc atctcttttc gtatcttatt ttagataaga tagaaaggag
     6121 atgttttttt gggaaaacga ttgatcaaag aagtacctac tagctcttta ccaagggaac
     6181 gtatggaaat ttatggcgcg gaagctttat cggatcagga gcttttggca atcttactgc
     6241 gtaccggtca acacccttat agtgtgatgt cgattgctgg aaatctgtta aaaacgtttg
     6301 gaggactggc ctctttacga caggctactt tgcatgagtt agaagagatt cgagggatag
     6361 gacgcgtgaa agcaatcgaa atcaaagcct tgatcgaatt aggtcgacga attcagacgg
     6421 atcatgaaag agcggctcca ccggttcgta ccagctacga attggcccag caattgattc
     6481 tggaaatgaa agatcacaaa caagaacatt tggtttgtat ttatttggac acgaagaatc
     6541 aagtgatctt aaaaaaaaca gtgttcatcg gttcgctgaa tcagtcagtc gctcatccta
     6601 gagaaatttt ccattatgct gtacgttatt gtgcagcaag gatcgtttta gcgcataatc
     6661 atccttccgg aatatataag ttagtacaca taaattatat aaggaaattt actgttatat
     6721 tgccatctgg aaatactatt atgttatcga taattgaacg ccataatgca tttttttctt
     6781 cttccgaaga ttctttataa atttcatcga attcttttga tagaataaat tcgacatttt
     6841 ttatatctat tttagctggc tctacttcaa tttcatttaa ttctttcatt aaagcttctc
     6901 gatctttttt gtattcctct aagtctataa cttcattcaa gtaggctttc ttcaatctat
     6961 ctaatttatt ctcaatattt ttcttactat ttttcttttt aataatcttt ggttgttttt
     7021 ttgaatagtt tatttgtatt tgttcaaatt tttgtttagt agcttcaaaa atataacgtt
     7081 ctaaattttt ttcgtaaaaa gcgcgcttat ttgtgcaacg taatttatta tttcttcgtt
     7141 ttacacatct ataacaagtc ctttcgtata tagattctgt accgtctttt ttcttatatc
     7201 ttgctttaat agtttgacca gctactgatc cctgacagtc agaacatttt aataaacctt
     7261 taaaaatgta atcacgtttt gcatttgttt ttaaattttt aggtaaggct ttttgaacag
     7321 cataaaaaag ttttttatca ataataggag gacagtaatt tttattattt cttaattcac
     7381 caatgtattt tttgttcgtc aacatatttt taacactttg ataatctcta acgattccat
     7441 actgagagcc taaataataa acagtttttc ttaaactacc actagaaaga aagtagttga
     7501 atatatctat cacgatagga gcatcatcat ttattactaa tttcttattt tctatagagt
     7561 agcctaacgg agttttcccg cttactactt caccttgtgc aattttattt gccataacag
     7621 ctttgatgcg ttctgaatca atttgggctt ccaattcagc aaaactcatt acttgtgcaa
     7681 tgaaagttct gccatacgct gtagttgtat cataatattg ttgagaaaca gcattccaag
     7741 aaacattgtg tttttctaaa atttcttgag tgtttaaata atgtcgtaga tttctaaacc
     7801 atctatctaa ttttgtaaat aaaattagat ctattttatt ttcttttaca tcttgtaata
     7861 gtttttgaaa ttcatcacga tttatttttt gaccagaaat accaccgtct acatattcat
     7921 taactacttt caaatccttg tgttgttcga tatattcata gagggtttct ttttgttctc
     7981 taagactatc tccatgtttg gcttgttgtt cagttgatac tctcatatat aatgctactc
     8041 gtttcatgtt atctcctttc gaatgtatgt tcttatcgtt gtaaaagaaa agcccgaagg
     8101 ctaatctttt tactacacat ctgttaattc taatgatctt ctgtattgtt cagcttgagc
     8161 tagtttagta aaagatacag ttccgttata attttctaaa acaacttttt caatttcatc
     8221 aagagatact ttaaagaatt cttttcgttg attaacttta ttgacttcgt tttttctaaa
     8281 atgcgaatgt agttgatttt ctaatgttgg tgcatcttct gaaaatatca ttgcatgaac
     8341 atcaaattca aaaggtacag aagcgctact aagttcctta attctatcca taggttctaa
     8401 ccgtctagtc attcctattt tatagacatc ttcaccgaaa gatccgatat tactgatgac
     8461 ataaacatag ccagctctag tattaagttc tctttgcaat acatcttctt tgacagtttc
     8521 tagttctctt aatttctctt ccagttcttt aattttatca gcatagagtt ctttttcaac
     8581 atcagcacta gctttttgca tgtatgccat taacttagtt atctctgagt taaactgtcg
     8641 ttcttcttta tcaatgcgtt tcttttcacg ttctatttca cgtcttacct tttcttcttc
     8701 aatcatttgt tcttttattg ctttttgctg ttctttttca tcgttaactt taactatgta
     8761 tgaatagttg agttctaatt cttgaagttt taattcaagt aattcttttt tgaattctgc
     8821 ttcatcgttt ttaaaaagct tgtttatttt atcaaaagtt cttataattt tttctctagt
     8881 tgaatctacg ttagaaatag taagtttatt gagaagtcca gttatttctg attctaatgg
     8941 tataagcatt ttctttttaa tattattagc ttctctatta ttcaaataag aaggaatatt
     9001 aattgcaccg tctaaaataa aatccttttc tttaatcttt aataaagaaa gtttgttttt
     9061 tatttcggat gaagaagttt catctatatt cggaataatt actgatttat agatagtgac
     9121 cagagatgtg tcatattttt gattaatttc atcgtctaac cgctggcttt cagcattaag
     9181 aatatcaatt ttgttttcta gcgttgacaa gtttttattc aatttactta tgtttttctc
     9241 tttttcagat ataaacgttt ccctagcatt taattcttga gaatattcct ttaccttact
     9301 ctctaaggca gaagttttat cttttaattt tttagcttca gataatttgc tactacccat
     9361 aattattaaa actattccaa ctaaaaacaa tagtactccc attcaaaacg cctcgcttct
     9421 tttataaaat aattgtcaaa atcatataaa cccttaccga agttgacggc tccggcatgg
     9481 gaattttttt aaaatacaac ttttccgata attctaatct gttctggcga tacaattcta
     9541 tcttcgtata agtcgtttat agaacgtaaa atgattgttt gattttcgta atctttatat
     9601 atctctttac atgtaactcc gtcaccacct aattccacaa tagcaatttc cccatcttct
     9661 acgtccggtt gttctttaac gaatacgaat tgtccattcc tgataacagg ttccattgaa
     9721 tcgcctataa taggtactgc gaagtcagca gcacttggaa ctctctcaaa ttcttcctct
     9781 tcgacaaccg tatcaccgta agtaagttct gttggattgg cagccgaagc cgaattgtga
     9841 acagttggga ttttttcttt tttaggaaaa tctaggacat tttcttgttt ttgttcttct
     9901 aatcgattac ttgcaaattc ataggtagca actttatttt cgtcgttaag ctgattatat
     9961 ataggaagga tattatatat ctgattagat atagataaat caatattcat caaatcatct
    10021 atatcaacat tgaatatctt agctatctca tttaaggttt ttaattttgg tgtatatttt
    10081 cctttttccc attcgcttat tgaggatgaa ctttttcttc ctagtttttc ggctagttct
    10141 tgttgatcaa tattatactt ttctctaaga aactttagat tttcagcgaa catagtaaca
    10201 cctccttgag ttaattgtaa caccgtttcg gaaaaaatgt aacaggtttc ttagaaaata
    10261 ttttagtgtt tttaaagaag tatttacgca aaatagaaat cgatttcagg aaaactgaaa
    10321 tatgcacttg acttcggaaa aaccgaagta tataatttag tcagaaagtt aaagagagga
    10381 ggcgaataaa atatggatac cgcgaagaaa gaaaaatata cattgagaca attaagagct
    10441 ttaaagggtt attcaaaaga agaactgtca aggaaaagca aagtgacatc aagaacaata
    10501 tttatttacg aaaacgatgt agataaaatg aggaacggaa agtacgccac tctagaaaaa
    10561 attgctaagg cattgggtgt tagagtttcc gatatttttt tagaccctga ttcggaaaaa
    10621 ccgaaatcaa taacaggtga aacaaaacaa gtcagctagg aggtgtgaat agtggaagag
    10681 aagaatataa aagtaccgac tcttgaagaa tatacaaaaa ttgttgtcga aacagacgag
    10741 cagaacccga aaacaatcgc agttattacg gcgaatgata ttgaatcatg cgaaggtttt
    10801 cgagttcgaa tgacccctag atatgattaa tgttctttat cacgtggagg tataggatcg
    10861 tttccaaaac tatcttttga ttgtatttgt ccattctttt tttggacaat aaactctgat
    10921 tgctgttttt tagaaatctt tttaccatac tcaacagcat ctgatttatt atcaaatagt
    10981 tttgtatctt cggaattacc ttcaccacgt acagcccatt tgccatttct aggagatacc
    11041 cattgatttt tacccataac aattcacctc gctttcaagt taattctatt aagaggtgaa
    11101 aagaaaacaa actgatggag gtgagattgt gagtaacaag ccaaaaatca ctttgaatgg
    11161 cacacctaat cttcaaagat tagcagatga aatggctaga atacgttcta gacattgggg
    11221 agttactgtg gtggcaacca taattgaaca aaaagaaaag cccacacagc cggcaagcaa
    11281 gtagtgggca ccatagaaca ataatattat atcacacact cgccacttcc gaaaagagga
    11341 gttgctgctc tcctttcaag tttgccaaag tccacgtttc ttcattttgg caactcctct
    11401 tcttggaggt ggtgacggcg agagacgaag gagggatgac atgaaggaag aatcaaaaat
    11461 agggattttg aatgagaaca attacacaag attaattatt gaaacagaag aaggggaaaa
    11521 agtagcagaa gttacattga ccgatgcaac tcctgctgac ggttatcgta ttcgattaac
    11581 tcctaaatat gattaacctt ttggtgggtg tggatcgttt ccgtgactat cacggctttg
    11641 aatttttcca tctctgccat gaataaatag ctcagatcct tgatttttag aaatctctct
    11701 agcgattttt actgcttctg attttgtatt agtatgtgca gtcgctttag aatttccagc
    11761 acctttgaca ttccaaccac cattggaatt aggtacaaca tgttgatttt ttcccatagt
    11821 tttcacctcc atttctatag aaatatttta tcaaatagaa atggagaaat tcaataaacg
    11881 aaaggaggga tcaaatgaca ttacaagagc atcaacactt gatgcgaaaa ctaaatcagg
    11941 aataccacga ggaaatgaaa cctagattgc taggggaaca atggcgtgaa cgccagaaga
    12001 agtggttaga aataaaaaag tgactcagcc gtccaaagca atgagtcaca aaccaaaaat
    12061 tatacctaag gagattttat cacatgaata agaaaattga aaatcttatc gaagaattga
    12121 aacgtgaatg ccaaaagcaa ggtgtttcta ttatttgtac agcccaaaaa gaaggggaac
    12181 ttaaaagtct cgtttatggt gagacaacag aaattttact ttgtcttgct atgcaagaag
    12241 aacatttaga tgaaaatctc ccattgtcag cacatattat gcgtagaatt gctgtggatg
    12301 cttacgaaca agcgaaaaat gaagaagaaa atcaaccttc aaatcatact tttgttatta
    12361 ataacaagga agatttagct gatgtgatga ctcgtattct caagggggaa tttcaatgat
    12421 gaatgaaaaa acaattgatc tactcattat tttatttttc acgttagtga ttgtagcgaa
    12481 aagttatgta atctttggct tgttattcgc aattaccatg cttgtgcttt gcgtgttagc
    12541 aagcagaaaa gaggttaaac atgaacgagt tagcaaatct tgacaactat ttaactgatc
    12601 ctgattatgc taaaccacca tatgaagcgc caattgatga ggaggatgaa gatgagtaaa
    12661 tctaccttag aaatgagcca tcaagaatgg ctcgaagacc gcaaaagagg tattggtggc
    12721 tcagatgttg caaccgtact tggattaaac aaatacaaat ctccttatca attgtggctt
    12781 gagaaaacgg gtcaaattga attgaaagat ttagaaagcg aaccagctta ttggggtaat
    12841 gttttagaag aagtggttgc taaagagttt caggaacgta caggcaaaaa agtacgcaga
    12901 agaaaccaag tatttgaaca tccattacat ccatttttaa gagcaaatat tgatcgggaa
    12961 gtagtgggag aaaatgccat tctggaatgt aaaacagcca atcaatttct cggaaaagag
    13021 tgggaaggtg aagaggtacc gcttagctat ctctgtcaag ttcagcatta catgaacgtt
    13081 ttaaacaaag actattgtta tatcgctgtt ttgatcggtg gacaaaaatt catttggaag
    13141 cggattgaac gagatcaaga gctgatcgat acaatcactg aacaattagt agagttttgg
    13201 gaaacgaacg ttcttggagg tatcgagcct gtaattgacg gaagtggagc gactgctgac
    13261 ttcttaaaag aaaaatatgc agatgtagaa gaaaatcaaa cagctctacc atcacgtttt
    13321 gatgaactta tcgagcaaaa aagagaactc aagcggacga aaaaagaaat tgaatcatct
    13381 attcgtcaag tagacaacga gatcatcagt gagctaggta aacgtgaagc cagtatcggt
    13441 atcacacaaa gaaacattat cagctggaaa cttgttcgta caagacggat gaacacgaag
    13501 aaactagcag agaaatatcc agatgtcgca aatgatgaag agatttataa cgttactgaa
    13561 tcaagaaggc taaccgaaaa ggagatcaaa taatatggca acaaatgaat cgttaaaaaa
    13621 tcaattggca gaaaagccac agaaacaagt tgcaccagga cagttagggc ttaaagctct
    13681 aatgaataca ccaacaatga gaaagaaatt tgaagaagta cttcatgaca atgctaatgc
    13741 ttttatgtcg aatgttatga ctcttgtatc taatgacagt tatcttgcag atagtgaacc
    13801 gatgtctatc atgagtggtg cgttaactgc tgcaacatta aatcttgggc tagataagaa
    13861 tttaggttat gcatatttag ttccattcaa tagtaaaaac aagcaaacag gaaaatggga
    13921 aaagaaagct caattcatgc ttggctataa aggatatatc caattagccc aacgatcagg
    13981 taaatacaaa gcattaaatg tgatcgaagt ttacgaagga gaactaaaaa gctggaaccg
    14041 actgacagaa gagtttgagt ttgatccaaa tggtagaacg tctgatgaag tcattggata
    14101 tgttggctat ttcgagttac tgaatggatt caaaaaaact gtctattgga ccaaacaaga
    14161 aatcgaagct catcggattg ctaacaataa agatcgcgat aagacaaagt taagtggtgt
    14221 gtgggcatct gattacaatg caatggcacg aaaaactgtt ttgagaaatc ttctttctaa
    14281 atggggaatc ttgtccatcg aaatgcaaga agccaccaca tcggatgaga gagtccaaag
    14341 ggttcaagaa gacggcagca ttattgctga aacagaagtt gaagaagata ttcctgaaag
    14401 aaaagaagca gaggttatat ctgaagaaaa tgaagatgta caaactggat tatttgatgc
    14461 atctaatccg ccgttaaaca aataatgagg gagctttctc ccttgctttt ctagaggaga
    14521 aatacgaatg aacacaggat atgtcaaatt acatcgaaaa gtgatgaatt cattcgtttg
    14581 gaccaatcct tatatgtata aattatggag cttgtgtttg atgaaagcga gtcatgagaa
    14641 tcgcaagata ctttttaatg gaaaagagat acaagtgaac agcggagaat tcgtcacagg
    14701 gcgcgatgct atcacatctg agatgaacga aggcgtgaaa cgtgaacatc aagtgaacag
    14761 cggttctgta tggagatggc taaaacaatt tgaaaaaaac ggaatgttga acatcaaatc
    14821 aactacgaaa tacagcgtta tatcaataaa aaactggtct ttgtaccaaa gcagtgaaca
    14881 acaaatgaac atcaaacgca caacaagtga acaacaagtg cacacaaaca agaatgaaaa
    14941 gaattataag aatgaaaaga ataataaaac atcatcgtcg caaccacgca aaaagcgtgt
    15001 ttacgacacc gactcagttt actacattct cgcggaggag ttattcaaac agatttgtca
    15061 gaatcaggaa atcaaaaagc cgaatctgca aagttgggca gataacattc ggaaaatgat
    15121 cgagatcgac aagcggaccg aaaaccaggt acggggaatg attgaatgga gtcagcacaa
    15181 tgtgttttgg gcatcgaata ttttatctgc taaaaaattg cgagaaaaat acgacacaat
    15241 ggcagcgcaa gcaaatcgtg attataaaac aaaacaaact aaaacgcttg aatacgagaa
    15301 atttagcaca gatgagttgc ctatttgaga ggaggcgtag gcatggaaac tgttggggaa
    15361 atcatggaaa agctgataca gaaagtgctt gtccaacgtg gcgaatgtcc tgaatgcgga
    15421 cagcctttgt atggatggcg tacgaagaac cctgatggct cagaacgttg taagccaacc
    15481 tgtatgcaat gtgggtataa agcattgcga gttcaggaag acttacaaac agaacgaatt
    15541 tacaacgaga gcctgaaagc aagagcaatc aattttttca aaggtggttc tgttgtgcct
    15601 aatcaagcgt tgtttgattg cacattgcag aattatcaaa ttgtcgatca agaaacaaga
    15661 caagcggttg aagtaaccaa acgctttgtt aattcagtct tgttaggaaa tccaagtcac
    15721 cttgttttaa ctggaaaaca aggaacaggt aaaagtcacc tagcgatggc agcggcttgg
    15781 gaagtcttga agcgatcaaa ctacgataaa aaaatcttat ttatcgggtt acaggaaatg
    15841 ctggatcaaa tcaaattttc gtacaacaat cctgaactca gaaaaacgat tgagggatcg
    15901 ttgattgcag atatcaaaac agcggattta gtcatcattg atgatatcgg ttcagaacta
    15961 ggaaaagatg catcagatag tcgagcgttt ggcataaaca cgctaaattc gttcttggat
    16021 gcacgacaga acctagcaac gattatcaca acgaacttgc ttggtgaaga actgaaaaaa
    16081 gcttacggta cgagaacaat atcaagaatg tttgtcaact ctgatggatt tacgatggta
    16141 ttttctcaaa cagcagacaa gcgcataaaa ccagtgaaag gtagtatcgc atgaataaat
    16201 accgtaataa aaaaactgtt catcgaggta tcaagtttga ttctatcgcg gaagcagagt
    16261 attacgatct agccttgtgg caagctgaag cgaatggttg gaaagtgaag ctccaggaaa
    16321 ggtttgagct gatgccgaag tttgaactag agggcaagaa gtatcgcaag atcgagtata
    16381 ttcccgactt cacattttac aaaagcggca agctggtcaa agtcgtagat gttaagggga
    16441 tgcagacaaa agactttaag atcaaggcaa agttgttctg tcatcaatat caagtgccgt
    16501 tgatattagc caaaaaatat cggaatacgt tcaaggaaga gcgtttttaa cgaggtggtc
    16561 catcatgaca acagaagaag tgattcaaat gcgaattcga agccttcggc gtgagattga
    16621 cgatctggaa cgaacaaagg cagtgatggt caatgaaacg gctagaaagg caatcgattt
    16681 gcacatagag aatttaagaa gggaaatccg tagattggag gaatgagcgt ggataaggaa
    16741 gcggcttggc gaaaattaat gttgctgatt caagatgaga actggcaaga agatgaagca
    16801 gtggttgctg aagttcagcg tctaggaaag attgctaacg gacgtatacg aaaaaagcca
    16861 gacaaaagaa aacagcgcaa agggaaaatc gtcgttgttt tacacgaagg caaaattttg
    16921 atgcaaggaa cagctagtga gctgtctgca gaaactggat atacgcgtgg gactattcga
    16981 acgtacgctt ggcgaaatca caccgatcga aaagggcacg aatataagta tttggaggaa
    17041 gaaaaatgaa cgaaaacaaa ttaatcaaat tgggtgtagc aggagcagta atcgtaggta
    17101 ttggagttat cggaggattt aagttcttcg aaaaaatcga taatggatat gtgggtgtgc
    17161 gctattcaat gaacggcggt atcaaagatg aagcactgac gcaaggtgtg aaatttgtag
    17221 ggattgacaa agtgatccaa tatccaattc gcttgcaaac tatccaatca aaaaatattt
    17281 cagtatctac aagcgacggc aaaaagacaa cgattgatat caaatatgac tacaaagttg
    17341 attcaactaa agcagcaaaa atgtacaagg aatttgggaa tatcacttcg gaagatatcg
    17401 aaagtggatg gttaaaatct aagcttcaaa aggtcgctcg tgaagtttat gcgaaatata
    17461 gtctgcttga tgtcctttca ggagattcct ctaaagttga agctgaggta ttaacgaact
    17521 ttgctaaatc agttgaatct aaagggtttg aagtcgaaga cgtaacactt ggtgttccag
    17581 atgtcgataa agaaacacaa aaatcaatcg atgcgatcat tcgagctggt caagaaaatg
    17641 aaaaagcgaa gctagacgca gaaactgcaa aaactcaagc tgatagtgaa gcttacaaga
    17701 aaacaaaagc tgcagaagca gaggcagaat ctaatcgcaa agtcgccgaa tcagtaacag
    17761 acaatttgat tcgttatgaa gaagctcaag ctcgcaaaaa gcatggatgg gtaacagtaa
    17821 atggagcaga tactgtagtt acggatgaag caggcaaata atatgggatt ctttatggct
    17881 aaaattctct tgttcttagg tttagtagga gcagcatatc tcgtgtatgc gctcctttcc
    17941 caaactgatg acaaagaaga tgacaacaac gatgaaatga aattttagga ggagaaataa
    18001 tggacgaact aatcacaaaa gtagagcagt gggctaaaga caagggactg gatcaagcag
    18061 atccaaaagc acagtttttg aaagtagctg aggaattcgg ggaaatagct tcggcgatgg
    18121 caagaagtaa tgatgaacta tttaaagata gcgtaggaga cgttatcgtc acgctgatta
    18181 tcctttccat gcaaaaaggg acaaacgtac aagagtgttt agaaatggca tacaacgaaa
    18241 tcaaaggacg cacagggaaa atggtagatg gtgtattcgt gaagtcgagt gatttggagg
    18301 atgtgaaatg aaatacgaaa taccactaag tgaagcgggc attcaagcaa ttatcaatgg
    18361 tcgggaggtt aacatagaac ttcctgatgg tactgaatta gtcatcagac aaagttattt
    18421 gaaagatatg gcagctccag tattaattga tcgttttaac gtgactgatt ctgtggtaga
    18481 gaaccactta aaagaatttc gatcaagtat agacaacact ttcagattag ggagttgatt
    18541 gacaatgaac accagacacc gcagagtagc aaaactaaga aaacaggaac taaatgtact
    18601 aaagacaaag tttgaaaaag aatatggaat ttcagcagaa gaaacatata aagtggcaag
    18661 tcagtttgtt gctgatgcaa gtgatgctat tcgtaagttt gggatttcga tattaaatga
    18721 tgatcgtaaa tgggaggaaa t
//
LOCUS       DACEKG010000055        17713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEKG010000055 DACEKG010000000
VERSION     DACEKG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.057"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..970
                     /locus_tag="IW664_001877"
     CDS             83..970
                     /locus_tag="IW664_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0849233.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IW664_001878"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IW664_001878"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0849234.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IW664_001879"
     CDS             2272..2718
                     /locus_tag="IW664_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0849235.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IW664_001880"
     CDS             2991..4343
                     /locus_tag="IW664_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0849236.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IW664_001881"
     CDS             4514..4801
                     /locus_tag="IW664_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849237.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IW664_001882"
     CDS             5027..5470
                     /locus_tag="IW664_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0849238.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IW664_001883"
     CDS             complement(5476..6660)
                     /locus_tag="IW664_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0849239.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IW664_001884"
     CDS             6928..7626
                     /locus_tag="IW664_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0849240.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IW664_001885"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IW664_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0849241.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IW664_001886"
     CDS             complement(8436..8969)
                     /locus_tag="IW664_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849242.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IW664_001887"
     CDS             complement(9266..11647)
                     /locus_tag="IW664_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0849243.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW664_001888"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW664_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0849244.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IW664_001889"
     CDS             12348..12887
                     /locus_tag="IW664_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0849245.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IW664_001890"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IW664_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0849246.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IW664_001891"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW664_001891"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IW664_001892"
     CDS             13994..15160
                     /locus_tag="IW664_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0849247.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IW664_001893"
     CDS             15206..16702
                     /locus_tag="IW664_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0849248.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IW664_001894"
     CDS             16783..17466
                     /locus_tag="IW664_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0849249.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         5978 a         3049 c         3670 g         5016 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt ttt
//
LOCUS       DACEKG010000056        17687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEKG010000056 DACEKG010000000
VERSION     DACEKG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.058"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(136..1716)
                     /locus_tag="IW664_001895"
     CDS             complement(136..1716)
                     /locus_tag="IW664_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0849250.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="IW664_001896"
     CDS             complement(2117..3493)
                     /locus_tag="IW664_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0849251.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="IW664_001897"
     CDS             complement(3621..4739)
                     /locus_tag="IW664_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0849252.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="IW664_001898"
     CDS             4881..5315
                     /locus_tag="IW664_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0849253.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="IW664_001899"
     CDS             complement(5393..5926)
                     /locus_tag="IW664_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0849254.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IW664_001900"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IW664_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0849255.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IW664_001901"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IW664_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0849256.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="IW664_001902"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="IW664_001902"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0849257.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="IW664_001903"
     CDS             complement(9651..10229)
                     /locus_tag="IW664_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0849258.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="IW664_001904"
     CDS             10551..11021
                     /locus_tag="IW664_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0849259.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="IW664_001905"
     CDS             complement(11129..12355)
                     /locus_tag="IW664_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0849260.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13109..13309)
                     /locus_tag="IW664_001906"
     CDS             complement(13109..13309)
                     /locus_tag="IW664_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0849261.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13443..14051)
                     /locus_tag="IW664_001907"
     CDS             complement(13443..14051)
                     /locus_tag="IW664_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0849262.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14136..14606)
                     /locus_tag="IW664_001908"
     CDS             complement(14136..14606)
                     /locus_tag="IW664_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0849263.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14654..15568)
                     /locus_tag="IW664_001909"
     CDS             complement(14654..15568)
                     /locus_tag="IW664_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0849264.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15714..16517
                     /locus_tag="IW664_001910"
     CDS             15714..16517
                     /locus_tag="IW664_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0849265.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16695..17642
                     /locus_tag="IW664_001911"
     CDS             16695..17642
                     /locus_tag="IW664_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0849266.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5363 a         3543 c         2911 g         5870 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagcgcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    13081 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
    13141 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
    13201 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
    13261 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatagc
    13321 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
    13381 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
    13441 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
    13501 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
    13561 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
    13621 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
    13681 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
    13741 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
    13801 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
    13861 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
    13921 tcaattgctg catttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
    13981 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
    14041 gtgtaagtca taaaaaaatt cctcctcaat gttttcttta taatgtctac attacaaaga
    14101 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
    14161 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
    14221 gctaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
    14281 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
    14341 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
    14401 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
    14461 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
    14521 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
    14581 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
    14641 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
    14701 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
    14761 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    14821 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacattct
    14881 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcaata gcaggtgcac
    14941 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    15001 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    15061 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    15121 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    15181 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    15241 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    15301 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    15361 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    15421 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    15481 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    15541 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    15601 taacattttt ttgatatttc accaatcatt cagatgtttc cctaaagcac ttgtatttca
    15661 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    15721 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag acgagaaatg
    15781 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    15841 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    15901 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    15961 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    16021 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    16081 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    16141 aacttcccta caccaatgaa tctttaaaaa acttaacagg taaggagctc cgttcttttt
    16201 taagtaaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    16261 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    16321 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    16381 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacaa
    16441 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    16501 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    16561 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    16621 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    16681 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    16741 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    16801 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    16861 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    16921 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    16981 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    17041 aaaaagcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    17101 gaaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    17161 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    17221 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    17281 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    17341 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    17401 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    17461 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    17521 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    17581 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    17641 aatagctgaa aacaatattt tcaaaagaac aaagagaagg tgacaaa
//
LOCUS       DACEKG010000057        17516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEKG010000057 DACEKG010000000
VERSION     DACEKG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.059"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..2427
                     /locus_tag="IW664_001912"
     CDS             100..2427
                     /locus_tag="IW664_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0849267.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="IW664_001913"
     CDS             2553..2882
                     /locus_tag="IW664_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0849268.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="IW664_001914"
     CDS             2922..3926
                     /locus_tag="IW664_001914"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0849269.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="IW664_001915"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="IW664_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="IW664_001916"
     CDS             5558..6730
                     /locus_tag="IW664_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0849270.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="IW664_001917"
     CDS             complement(6892..7347)
                     /locus_tag="IW664_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849271.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="IW664_001918"
     CDS             7936..8358
                     /locus_tag="IW664_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0849272.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="IW664_001919"
     CDS             complement(8524..9717)
                     /locus_tag="IW664_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0849273.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="IW664_001920"
     CDS             9941..10318
                     /locus_tag="IW664_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0849274.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="IW664_001921"
     CDS             10306..10644
                     /locus_tag="IW664_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0849275.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="IW664_001922"
     CDS             10778..11221
                     /locus_tag="IW664_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0849276.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="IW664_001923"
     CDS             11483..12298
                     /locus_tag="IW664_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0849277.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="IW664_001924"
     CDS             12308..12970
                     /locus_tag="IW664_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0849278.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14839
                     /gene="alsS"
                     /locus_tag="IW664_001925"
     CDS             13175..14839
                     /gene="alsS"
                     /locus_tag="IW664_001925"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0849279.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14852..15562
                     /gene="budA"
                     /locus_tag="IW664_001926"
     CDS             14852..15562
                     /gene="budA"
                     /locus_tag="IW664_001926"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0849280.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15692..16189
                     /locus_tag="IW664_001927"
     CDS             15692..16189
                     /locus_tag="IW664_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0849281.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16374..16634
                     /locus_tag="IW664_001928"
     CDS             16374..16634
                     /locus_tag="IW664_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849282.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16996..17235)
                     /locus_tag="IW664_001929"
     CDS             complement(16996..17235)
                     /locus_tag="IW664_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849283.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5944 a         3045 c         3473 g         5054 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
    14101 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
    14161 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
    14221 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
    14281 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
    14341 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
    14401 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
    14461 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
    14521 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
    14581 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
    14641 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
    14701 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
    14761 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
    14821 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
    14881 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
    14941 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
    15001 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
    15061 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
    15121 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
    15181 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
    15241 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
    15301 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
    15361 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
    15421 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
    15481 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
    15541 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
    15601 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
    15661 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
    15721 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
    15781 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
    15841 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
    15901 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
    15961 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
    16021 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
    16081 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
    16141 tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa atcgtctatt
    16201 gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg ggcatttctg
    16261 tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc ataatcatat
    16321 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
    16381 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
    16441 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
    16501 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
    16561 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
    16621 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
    16681 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
    16741 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
    16801 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
    16861 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
    16921 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
    16981 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
    17041 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
    17101 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
    17161 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
    17221 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
    17281 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
    17341 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
    17401 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
    17461 tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg aggata
//
LOCUS       DACEKG010000058        17081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEKG010000058 DACEKG010000000
VERSION     DACEKG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.060"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..538)
                     /locus_tag="IW664_001930"
     CDS             complement(17..538)
                     /locus_tag="IW664_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0849284.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="IW664_001931"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="IW664_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0849285.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="IW664_001932"
     CDS             4618..5505
                     /locus_tag="IW664_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0849286.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="IW664_001933"
     CDS             5502..6671
                     /locus_tag="IW664_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849287.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="IW664_001934"
     CDS             6695..8050
                     /locus_tag="IW664_001934"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0849288.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="IW664_001935"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="IW664_001935"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0849289.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="IW664_001936"
     CDS             10336..10626
                     /locus_tag="IW664_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849290.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="IW664_001937"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="IW664_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0849291.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="IW664_001938"
     CDS             11667..12326
                     /locus_tag="IW664_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0849292.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="IW664_001939"
     CDS             12483..13415
                     /locus_tag="IW664_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849293.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /locus_tag="IW664_001940"
     CDS             13569..14858
                     /locus_tag="IW664_001940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0849294.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="IW664_001941"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="IW664_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0849295.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /locus_tag="IW664_001942"
     CDS             16438..17025
                     /locus_tag="IW664_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0849296.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5587 a         3065 c         3740 g         4689 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtattgaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag t
//
LOCUS       DACEKG010000059        16770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEKG010000059 DACEKG010000000
VERSION     DACEKG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.061"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(287..649)
                     /locus_tag="IW664_001943"
     CDS             complement(287..649)
                     /locus_tag="IW664_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849297.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IW664_001944"
     CDS             complement(723..1103)
                     /locus_tag="IW664_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0849298.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IW664_001945"
     CDS             1185..2360
                     /locus_tag="IW664_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0849299.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IW664_001946"
     CDS             complement(2437..3021)
                     /locus_tag="IW664_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0849300.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IW664_001947"
     CDS             3222..4220
                     /locus_tag="IW664_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0849301.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IW664_001948"
     CDS             4332..4841
                     /locus_tag="IW664_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0849302.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IW664_001949"
     CDS             complement(4871..5530)
                     /locus_tag="IW664_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0849303.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IW664_001950"
     CDS             complement(5532..6296)
                     /locus_tag="IW664_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0849304.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IW664_001951"
     CDS             complement(6304..6981)
                     /locus_tag="IW664_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0849305.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IW664_001952"
     CDS             complement(7018..8403)
                     /locus_tag="IW664_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0849306.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IW664_001953"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW664_001953"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IW664_001954"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IW664_001954"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0849307.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IW664_001955"
     CDS             9342..10199
                     /locus_tag="IW664_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0849308.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IW664_001956"
     CDS             complement(10280..10615)
                     /locus_tag="IW664_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849309.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IW664_001957"
     CDS             complement(10682..12052)
                     /locus_tag="IW664_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0849310.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IW664_001958"
     CDS             complement(12130..12666)
                     /locus_tag="IW664_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0849311.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IW664_001959"
     CDS             12975..14456
                     /locus_tag="IW664_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0849312.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="IW664_001960"
     CDS             complement(14559..15266)
                     /locus_tag="IW664_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0849313.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="IW664_001961"
     CDS             complement(15345..15683)
                     /locus_tag="IW664_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0849314.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="IW664_001962"
     CDS             complement(15794..16756)
                     /locus_tag="IW664_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0849315.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
BASE COUNT         5091 a         3402 c         2747 g         5530 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaatat cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt
//
LOCUS       DACEKG010000060        16597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEKG010000060 DACEKG010000000
VERSION     DACEKG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.062"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..779)
                     /locus_tag="IW664_001963"
     CDS             complement(45..779)
                     /locus_tag="IW664_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0849316.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="IW664_001964"
     CDS             1105..1515
                     /locus_tag="IW664_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0849317.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="IW664_001965"
     CDS             1508..2209
                     /locus_tag="IW664_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0849318.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="IW664_001966"
     CDS             2209..3822
                     /locus_tag="IW664_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0849319.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="IW664_001967"
     CDS             3812..4033
                     /locus_tag="IW664_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0849320.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="IW664_001968"
     CDS             4030..4791
                     /locus_tag="IW664_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0849321.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="IW664_001969"
     CDS             5172..5681
                     /locus_tag="IW664_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0849322.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="IW664_001970"
     CDS             complement(5751..6290)
                     /locus_tag="IW664_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0849323.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IW664_001971"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IW664_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0849324.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8154
                     /locus_tag="IW664_001972"
     CDS             7357..8154
                     /locus_tag="IW664_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0849325.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8182..8817
                     /locus_tag="IW664_001973"
     CDS             8182..8817
                     /locus_tag="IW664_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0849326.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="IW664_001974"
     CDS             8837..9610
                     /locus_tag="IW664_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0849327.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="IW664_001975"
     CDS             10018..10815
                     /locus_tag="IW664_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0849328.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="IW664_001976"
     CDS             10793..11206
                     /locus_tag="IW664_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849329.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="IW664_001977"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="IW664_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0849330.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="IW664_001978"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="IW664_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0849331.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /locus_tag="IW664_001979"
     CDS             15983..16543
                     /locus_tag="IW664_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0849332.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5387 a         2964 c         3599 g         4647 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc cgttttt
//
LOCUS       DACEKG010000061        16528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEKG010000061 DACEKG010000000
VERSION     DACEKG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.063"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..417)
                     /locus_tag="IW664_001980"
     CDS             complement(142..417)
                     /locus_tag="IW664_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0849333.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /locus_tag="IW664_001981"
     CDS             complement(660..1970)
                     /locus_tag="IW664_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0849334.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW664_001982"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW664_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0849335.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="IW664_001983"
     CDS             complement(3516..4196)
                     /locus_tag="IW664_001983"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0849336.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="IW664_001984"
     CDS             complement(4245..4880)
                     /locus_tag="IW664_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0849337.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="IW664_001985"
     CDS             complement(4946..6349)
                     /locus_tag="IW664_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0849338.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="IW664_001986"
     CDS             complement(6336..7367)
                     /locus_tag="IW664_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849339.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="IW664_001987"
     CDS             7398..7619
                     /locus_tag="IW664_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0849340.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="IW664_001988"
     CDS             complement(7784..8521)
                     /locus_tag="IW664_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0849341.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8674..10014)
                     /locus_tag="IW664_001989"
     CDS             complement(8674..10014)
                     /locus_tag="IW664_001989"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0849342.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10251..11774)
                     /locus_tag="IW664_001990"
     CDS             complement(10251..11774)
                     /locus_tag="IW664_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0849343.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="IW664_001991"
     CDS             complement(12152..12763)
                     /locus_tag="IW664_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0849344.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="IW664_001992"
     CDS             complement(13093..13809)
                     /locus_tag="IW664_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0849345.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW664_001993"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW664_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0849346.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="IW664_001994"
     CDS             complement(14368..15165)
                     /locus_tag="IW664_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0849347.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="IW664_001995"
     CDS             complement(15135..15506)
                     /locus_tag="IW664_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0849348.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW664_001996"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW664_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0849349.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5508 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
     8641 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
     8701 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
     8761 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
     8821 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
     8881 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
     8941 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
     9001 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
     9061 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
     9121 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
     9181 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
     9241 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
     9301 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
     9361 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
     9421 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
     9481 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
     9541 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
     9601 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
     9661 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
     9721 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
     9781 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
     9841 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
     9901 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
     9961 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    10021 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    10081 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    10141 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    10201 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    10261 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    10321 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    10381 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    10441 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    10501 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    10561 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    10621 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    10681 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    10741 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    10801 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    10861 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    10921 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    10981 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    11041 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    11101 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    11161 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    11221 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    11281 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    11341 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    11401 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    11461 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    11521 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    11581 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    11641 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    11701 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    11761 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    11821 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    11881 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    11941 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    12001 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    12061 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tttctttt
//
LOCUS       DACEKG010000062        16280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEKG010000062 DACEKG010000000
VERSION     DACEKG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.064"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(65..721)
                     /locus_tag="IW664_001997"
     CDS             complement(65..721)
                     /locus_tag="IW664_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0849350.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            914..1192
                     /locus_tag="IW664_001998"
     CDS             914..1192
                     /locus_tag="IW664_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849351.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1211..2419
                     /locus_tag="IW664_001999"
     CDS             1211..2419
                     /locus_tag="IW664_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0849352.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2567..2932)
                     /locus_tag="IW664_002000"
     CDS             complement(2567..2932)
                     /locus_tag="IW664_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0849353.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3079..3852)
                     /locus_tag="IW664_002001"
     CDS             complement(3079..3852)
                     /locus_tag="IW664_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0849354.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4026..4886)
                     /locus_tag="IW664_002002"
     CDS             complement(4026..4886)
                     /locus_tag="IW664_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0849355.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4886..6304)
                     /locus_tag="IW664_002003"
     CDS             complement(4886..6304)
                     /locus_tag="IW664_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0849356.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6361..6810)
                     /locus_tag="IW664_002004"
     CDS             complement(6361..6810)
                     /locus_tag="IW664_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0849357.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7255..7518)
                     /locus_tag="IW664_002005"
     CDS             complement(7255..7518)
                     /locus_tag="IW664_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0849358.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7617..8912)
                     /locus_tag="IW664_002006"
     CDS             complement(7617..8912)
                     /locus_tag="IW664_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0849359.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8909..10180)
                     /locus_tag="IW664_002007"
     CDS             complement(8909..10180)
                     /locus_tag="IW664_002007"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0849360.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10345..11214)
                     /locus_tag="IW664_002008"
     CDS             complement(10345..11214)
                     /locus_tag="IW664_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0849361.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11506..13116)
                     /locus_tag="IW664_002009"
     CDS             complement(11506..13116)
                     /locus_tag="IW664_002009"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0849362.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14309..15649)
                     /gene="glnA"
                     /locus_tag="IW664_002010"
     CDS             complement(14309..15649)
                     /gene="glnA"
                     /locus_tag="IW664_002010"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0849363.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15677..16069)
                     /locus_tag="IW664_002011"
     CDS             complement(15677..16069)
                     /locus_tag="IW664_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0849364.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4640 a         3451 c         2766 g         5423 t
ORIGIN      
        1 ctatttttcc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttg
       61 ttcatcaaaa gataatattg tcttctaaca agtccatatt caaaataacg atatgatggt
      121 ctttaacggt gataatacca tcttttttca gttgttttaa aattctgttt acactgctag
      181 cagttgagat gccacaaaat ttggcgattt cttcattcgt gatcgaaaaa tcgatcagca
      241 tcccttcttc tgtccgcaca ccgaataaat catataattc gtaaagttgc gtacaaacag
      301 ctcccagctt tccgtttaat agcatttgct gcattttttt catcgaataa agcagacgat
      361 agcggtaata ctcttttaca tagtgttgta gctttgggtc ttggttgata ttttgccaaa
      421 agcgcacacg attgattcga taaaactctg catattctga ttcaactcga atattatacg
      481 gcgaatcgat gaattgtgaa tattcgtctt ttaaaagaga gacgatttca agatctttga
      541 tatagcgaag gttaaattct ctcccatcat ttgaaataac acttgctttt accgttccct
      601 ctttcaagat atacgtatgg gaatcttcca tcccttcgaa tgtcaaatat ttatgatatc
      661 ctttgcgtat aatcggaaaa tcatttttat ccaaatattc ctttaagact ctactatcca
      721 atgatgctcc cccatccaaa ctttcatcta aaaagatcat acagtacttt aaaacttttt
      781 aaagtccaaa ggtattttac taatgatatt aacatttgtt aagtaccttt ttccatcact
      841 tatgcgattt ttttcctttt ttttattcgt tatactgcag aagtaaaatt ttttaagaat
      901 agagggaaaa aacatgaatt actggatgaa cacaatcatt aatcgattag aaacagcata
      961 tcaaacaaga tttgatatga aagcttcttt agtcttttta aatgatgcgt atcaaaactc
     1021 gatcgaattg atcaaagcag tggatgagaa tccaacaaac gaatgcgaag aatttttaaa
     1081 tctgtttatg tctacaagag atttatttat tcgccaactt gttgatcgct atccatccaa
     1141 ttatcacgat gtagaagttc aaatacaaaa attaaaagca tattcagctt aaggggaatg
     1201 tataacatac atggaaaaga aattatttaa taaatatttt attggaatca cattgatcaa
     1261 ttttattgtc tacctgattt attatctgtt gatggtcatc atggcagtca ttgcgcaaga
     1321 caccttacac gccagcttga gccaagcagg ttttgcttct gggatctata tcatcggaac
     1381 attgctggca cgcttattca tggggaaaca gttagaacta tttggtcgga agtttacctt
     1441 aagaggcggc gcgatcttct atctcctttc aacattagca tatctttata cgccaactat
     1501 cggggcattg tatttagttc gattcttgaa tggattcggc tacggtacag tttctacagc
     1561 tacaaatgct atcgttactg catatatccc tgcatctaaa aaaggggaag gcatcaatta
     1621 ttatggatta agtactagtt tagctgccgc tgttgggcca tttttaggaa tgattttgct
     1681 gaatatgact gattttcgtt tcattatctg gttttcaatt gtactggtct tctttgttac
     1741 gattgcctgc cttgtctttc ctgtaaaaaa tattccatta tctgaagaac aaaaaatatc
     1801 tcttcgctca tggaatatca atagttttat tgaaaagaaa gcacttttca ttacaggaat
     1861 tgcctttttg atgggtcttt cttattcaag tgttttatca tttttatctt cttatacaaa
     1921 agtaatccac ttagttgctg ctgggtcatt tttcttcgtc gtatatgctt tggtcatcac
     1981 atttacacgt ccattgactg gaaggatttt cgatgtcaaa ggagaacaat acgtgatgta
     2041 tcctagctat ttattcttaa cagcaggatt atttttactg agtgtaacga cgaatagtgt
     2101 gacattgctt atctctggag cattagtcgg cctaggttat ggtacgttta tgtcaaatgg
     2161 acaggctgtt tgtttaaaaa tcgtccgcga acatcgaatc agtattgctt tatctaccta
     2221 ctttattggt ttagacctag gcttaggcgt tggtccttat ttgatgggga tgctaaaatc
     2281 tcttacttca tttagaggat tgtatgtgat tgccggagtc attccattga tatgtacagc
     2341 actctatctt gttaatggcc gaaaacatag tcatgaagca gagggcgaat tgattgaatc
     2401 attgaaaaat gaaatttgat cttgatgaat agataaaaaa aaccgttcag atctgaaaat
     2461 aatctgagcg gttttttgag taaaaatagt ttatgattat tcttaattaa acatctattt
     2521 atttttaaag ctttcattta tcgataagaa tagtttaact gttgatttat tccattttcc
     2581 gttcatctgg tacgatgcct tgacttagca accacttgtg gaagaaccat tctgccacac
     2641 cgatcaatag agcaggaatc aatactttca tccaaatatc gactgtatca ttcataaaat
     2701 atagatagag ccacaagata actgcagctg aaccagcatc tgcgatcgtc gctgcgatgt
     2761 tccctacttt acgtaagatc aacagatccc ctagcacata gatcatgact acaataatta
     2821 ccccaacaat cgttgaatcc ataaagctaa caccgtataa acctgtcagc acgacccaca
     2881 gaatcgccca gatcatgatt gccttcacta ccaacgcctt cacatgtttc attttctgca
     2941 ctcctttctc ctggataaaa aagtaagatt atctaacaag ataacctttt cattattata
     3001 ccatcggctg agagaagcga gcaaagataa ggtcctatct aaaaatgagg aagccactta
     3061 gaacattcac gtaataattt atccgtacag acattcgata tggtgttcct ttactttcgc
     3121 atactgttct tttgtcagtt ctttacttgt caccagataa tcgatttcat gaaaagcaat
     3181 ggcttgatgc atgaatttat gtgcaatttt ttgctgtgta actaaaacgg caagccggtc
     3241 tgattgtttt ctaaaagctt ttttgaccat tgcctctcct tcatgagttt ctgttactcc
     3301 ttggctgaaa tctagcccgc gacaactcat tatcgctaaa tccgcataat atgacaatgc
     3361 atcgttatag gcttttgaac cgttgattgt tgctcgtttc ggaacaattg cccctcccaa
     3421 tacagatact tttgccgttg tatattcact caacatagca gctgtgacga tgccattcgt
     3481 aatgatctgc aaaccatcaa atcttgctag gtttctgact aagtgtaaag aggttgtact
     3541 ggaatccaag aataagacca tatcatctcg aatcaaagaa gaagctgtat ctgcaatctt
     3601 acgtttttct gtttcttctt ctaatttggt gatcttcatc aggtaaggca cgtcaatttt
     3661 tcgattgtca ataagcatgg cgccaccgcg aactctacgg atcaatccac ttttttccag
     3721 aaaaacaagg tcacgtttaa tcgtggcttc gctataattt actaactcga tcaaatcggt
     3781 gactcttgca aattggttgt tttttagata attcacaatc tcctgctgtc tattctccac
     3841 ttcattcaac ataaaatcac cccatttact ttatatagta acaggaaaaa agaggttgtg
     3901 acagaagtga ttggcttcga gaaataagaa gaaatttttc gaaaaaacta cttctgaaca
     3961 tcgtgtgtgg ttaagagggt atgacaagac tattgtcata ccctcttgac cacacatttt
     4021 tatttttagt ataaactagc cttgcctact gtgttcaaaa tttccatttt atgtttgacc
     4081 acttcttgta cctttttatt agctgctgga taaattgcat tcggctcgta catcgatgga
     4141 ttttcactca atatttctct caaagcattg aaaaagacgc ttttaagatc tgagctcaaa
     4201 ttcactttgg caactccgta ttttactgct tcgctgactt ctgcgtccgg attacctgaa
     4261 ccgccatgta aaacaaatgg aagatcgatt tgttggttca attctttcag taagtcgata
     4321 tttaattttg gagttctgtt tttcggatac aagccatgcg ctgttccaat tgctacagca
     4381 agtgtatcga ttcctgtttc tttcacaaaa atcttcgctt gatcaggatc tgtataaatg
     4441 attgtttcag cacctccttc agcagaacca ttattcccaa tcgttccaag ctcagcctcc
     4501 actgagacat ttaccgcatg agctaacgca accacttttt tcgtaatctg tacattgtcg
     4561 tcgaatgatt tcaaagacgc atcgatcatc acggaagaat agccgtttcg aatcgcccgt
     4621 aagacatctt ctatcgtacc gccatgatcc aagtggataa cgatcggcag tttacttgta
     4681 taagcatagg ctcttactgc cgcaacaaac tcatcactta aataggcgat ttcatcagga
     4741 tgaatttcta agattactgg tgcttctaat ctctctgctt cttcgatgat tgctttcatc
     4801 atatcatagc tgcaaatatt gaaggctggt actgcaaatt gattctcttt agccactttc
     4861 aataactctt tcattgtata taacattacg cttccacctc atttgttaga ttttcatttt
     4921 tcgtccactt gtcttgtttt ttctctttca ctgttttttt caatagtcca agcataaagg
     4981 ctgtagtgac tgtacctgat gcaattgcca agacatacat aggtaaattg ttcataacat
     5041 ttggaattag catcacccaa atccctccat gaggtgccat tgaagtaact cctactgcca
     5101 tggaaatccc tgcagcgact gctgaacctg tcatcaaact aggtatcacg cggatcgggt
     5161 cagataccgc aaacggtata gcaccttctg taatatacga agcgcctaaa atccagcatg
     5221 attgacctga agtccgttct tctttggtaa atttatgttt gaataataaa gtggctaaag
     5281 caatacctag gggaggtgtc attcctgcag ccatacaagc agcaatcggt gcatttactc
     5341 cagctgacat caaaccgatc gagaaagtcg aagtcgattt attgatcggc ccacccatat
     5401 cagctgccat catgatcccg ataagtaatc cgaacaatag cccggatgta tttcccaagc
     5461 tattcagcca atttgtcaaa gcatccaaca cccatgtgat cggttgttca atcacaaata
     5521 ccatcaaaag cccaataatt aagatcgata aaaaggggac gacaatcagc tgataaatag
     5581 cagaaaattc tttttttacg ctaattttat ttgccagaaa atcacagata taccctgcta
     5641 gcacgccacc aatcattccg ccaagaaaac ctgaaccacc tgtagaggcc agtaatccgg
     5701 caacaattcc tggggcaaaa cctgcttttc cagcaatcga tgtagcaatc cccgcgccta
     5761 acgcaggaac catcatggca aacgctgtat ctcctccgat ccttgaaaat gcagctgcta
     5821 acacattata gtctgctgag ttgggatcag ccgctgtaat cccaaaggca aaactcagtg
     5881 cgatgatgat tccgcctgcg atcacaaatg gaagcatgta attcacacca ttcatgaaat
     5941 gattatagat tgtcggcttt ttctcatttg cagctggttc tttttcatta gacacaacga
     6001 atacgccaat atctctcaat gcttcgtcta ttactacttg cggttctttg attgctcggc
     6061 ttgtagagac acgctttact tttttcccgg caaatcggtc caattcgatt tctttatcta
     6121 ctgcaagaat gattgcatct gcctctttga tttcttgttc agataactga ttttctgttt
     6181 ttacaccttg tgtttcgaat tttgcttgat ggtttagatt tcttgctgtt acttctaatt
     6241 tttctgccga catataagta tgagcaattc cagtcgggca agcagttaca ccaacgattt
     6301 tcatgattat tttcctcctt tttcttcact atcgatactc ggacaaagac tctactattt
     6361 tcattcttga ctaatctgtc tgatgatatc tgctggcgta gtggcttctt ttattccctt
     6421 tctaaaatct gcattcatca atcgtctgga gagtgtgctg agaatctcta gatgcatatc
     6481 tgctgatttt tcaggaacta cgatggcaaa aatatattca attggcattt catctatccc
     6541 gctatagtcc gttaatttgg ctgtatgaaa aaaacacagt gctggttttg tcacatattt
     6601 gctttttgca tggggaatcc caaatccatc ttctattcct gtggagatct cctcctctcg
     6661 tttgaatagt gccttaacga ctttttgaac atttcttacc ttatttgttt ttttcaacac
     6721 atcccctatt cgctcaaata atgatccttt tgaatctatc gatggatcga aaacaatcag
     6781 ttcttctgcg atcaattgat caaacctcat aaaaaacctc tcccccacaa aaaatgttaa
     6841 cgttttcttg gaactgaggt cagtatagca tcgctagctg taccgttcaa tattatgaat
     6901 gaacagagga aaaagaccca tcatccgcgt gtataagtca ttaaaatgaa cgatctagct
     6961 cacaaacaat cgcaagtcta taacaaaaca aaaagccttc cttgaaagga aagcttagct
     7021 tctaatttta ggtaaatggt tttttacttc gaaataaccc ctcttcattt cgttaatata
     7081 aggtgttttt atcaagtgga taattaaaac tatatactag tttttcattg ttttgtgcat
     7141 tatgatccta cgactttttc agcatcaaac gaagttttaa attggcaaaa aacataaaaa
     7201 aacagactat ccctatcaga tagtctgcta aacgttgagt tatcagcgtt tttattattc
     7261 cgcagcgttt gcgtctttga gaccgtattt tttgttgaaa cggtccacac gtccgtctgc
     7321 ttgtgtgaat ttttgacgtc cagtgtagaa tggatgtgag tcagaagtta cttcgacacg
     7381 gatgactggg tatgtattac cgtcttccca ttcaactgtt tcttgtgaag tttttgttga
     7441 acctgacaag aatttgaaac cagttgttga atccatgaat actactgggt ggtaatctgg
     7501 atggatgttt tctttcatga ttttttcgct cctttgccct gctccatttg ctgaaccaga
     7561 gttgatttgt cgatttacaa cattaacatc ttaacatgtt tcagtcaagg ttgcaattat
     7621 cttttcagat ttctggtcgg atttttttta ccaaaggaga catcttgaaa ttctttgaaa
     7681 aattgctgat tgttttttgt tttacgcaaa aagcggatca gttgttccgt atattctaat
     7741 gaatctccag tcatattttt tcgaagtttc caaatttctt ctaattcttc acttggcatc
     7801 aataattctt cttttcgggt acctgatttt ttgatatcga tggctgggaa gatccgacgt
     7861 tcagataatt cacgtgaaag ttgtaattct agatttccag ttcctttgaa ttcttcatag
     7921 atcacgtcat ccatacggct tcctgtatcg actaaagcgg tcgctaaaat cgtcaaactg
     7981 ccaccttctt cgatgtttct tgctgccccg aaaaatttct taggtttata caatgcggct
     8041 gggtcaatcc cgccactcaa tgtacgtccg cttggtggaa cgactaagtt ataggcccga
     8101 gcaagtctag taatactatc catcaagata acgacatctc gtttatcttc gactaaacgc
     8161 attgcgcgtt ccaatactaa ttcagaaact cgagtatggt tttgcggctg cagatcaaat
     8221 gtagaagata ccacgtcgcc tttcacgctt cgttctagat ccgttacttc ttccggtcgt
     8281 tcatcaatca gtagaacgat taattctact tccggatagt tttcggtgat accatttgcg
     8341 atttctttta atacagaagt tttccctgct tttggcggtg cgacgatcaa tccacgctga
     8401 ccaaaaccga ttggagaaaa gatatcgatc attcgggtag atagtctttg cggtgttgtt
     8461 tccagttgaa gctgtctttg cggataaaga ggtgttaagg ctggaaaatg cggacgttct
     8521 tttgcttctt ccggatcttt tccattaacg ctttccacgt gcatcaaacc ataataacgt
     8581 tccgattctt taggtggacg ggcttttcct gccactttgt ccccatttct caaactgaat
     8641 cgtctgattt gtgaggcaga aatatagata tcttctgcac ttggtccata attgattggc
     8701 cgtaaaaaac cgtaaccatc ttgaccaacg atatccaata ttccttccat ataataaaat
     8761 ccttgtttct ctgcttgtgc acgaatcaca gctagagata attccttttt attcatctta
     8821 ctgtaataag gaattttaaa ttctttggca taactataaa cttcttttaa cgttttgttc
     8881 tctagttctg ccattgtcaa ataatcgctc attcagctcc cacttcctct atcatttcaa
     8941 ttgatgcccc taaggctgtt agtttctcga taatatgatc ataaccgcgt aagatataat
     9001 ctacattgta aatcgtcgtt gtcccttctg ccaatagtcc agcaatcact aaacaagcac
     9061 ctgcccgaag atcagaagcc acgacttctg tgccgtgaag tttatttgga ccattcacga
     9121 tgatcatatt tccttccacg gtaatatcag cgcccattcg tgctagttcc ggcacatggt
     9181 tcacacgttt cgcatagatc gtatcgatga tttctgaagt gcctgtagtc atcaatagta
     9241 acgcagtaag tggctgctgc agatctgtgg caaatcctgg ataaggatag gtcatgatgt
     9301 ttgcgggttt taattctttc gaggggtaaa cttcgatttc atcttcgctg attttcatat
     9361 ggacaccaat ttcctgcaat tttgctataa agctttccaa atgttcaaag atcacatttt
     9421 tcactttgat accatttcct actgcagctg ctaatgctag ataggttcct gcttcgatgc
     9481 ggtcaggaat catgaaatgc cgacagccat gtaatgtttc gactccttcg atacggatca
     9541 catctgtacc cgctcctcga acttttgccc ccatattatt cagtaaagtt gccacatcta
     9601 tgatttccgg ttctcgtgca gcgttttcaa ttactgtctg tccttttgct tttacagcag
     9661 ctaacatcac attgatcgtt gctccgactg aaaccacgtc catgaaaatc cgattgccac
     9721 gcaatccttt attttctgtc cgtaaataca tggcaccatg ttcatttgtc acttcagcac
     9781 ctagagcttc aaatcctttg acgtgtaggt caatcggtct agggcctaaa taacagccac
     9841 ctggcaggcc tactaccgct tcgccaaatt taccaagcag tgttcccata aaataataag
     9901 aagcacgcaa actattgatt tttccgtgcg gcatcggaat cgatacgact tctcgaggat
     9961 cgatttctag tatgttgttg gaaaaatgga cagttgcgcc catgatctct aaaatttcaa
    10021 ttaatgaatg aacatcttgt atgtccggca caccttccaa gatcactggg gaatcagcta
    10081 agattgcagc tggaatcaat gcgactgcac tattttttgc accgctgatc gttacttcac
    10141 cagctaaagg tcggtttcct ttgattacga tttttttcat ctgtttccac cttgtttatt
    10201 aatctttttt catggatatg cttgtatttt atcatagata aaaaaaagaa acgagcatgg
    10261 tctgccgttt taagtaaaaa agaatggaga gcgacttcaa aagtcgttct ccattcctat
    10321 atatcgtatc tgttatgtgg atgcttacgc tttgttagct gaaccgaacc agtcgatatg
    10381 agatttagca tcttcaacga ttgctgcttt acctggagct aataatttac gagggtcgaa
    10441 tcctttacct tctttatctt taccagcttc gatatattta cgagtagctg cagcaaatga
    10501 taattggaat tcagtgttaa catttacttt tgagataccc attgagatag ctttttcgat
    10561 ttgatcttga ggaatacctg atccgccatg taatactaaa ggcatgtctg gacctacagc
    10621 atcagcaatt gcttgtaagt ggtcaaaagc taatcctttc cagttttctg gatattgacc
    10681 atggatgtta ccgataccgc aagctaagta gtcgatacct gtagcaacca tttgtttgca
    10741 ttcttcaata tcagctaatt cgccgttacc gatgattccg tcttcttctc cgccgattga
    10801 accaacttca cattcaactg agataccttt tgcatgtgct ttttcaacaa cgtctttagc
    10861 taatttcaag ttttcttcaa atggtagatg tgaaccatcg aacattactg aagtatagcc
    10921 taattcgata cattccaaag cagcttcgta atcaccatgg tctaagtgga tagcaactgg
    10981 aacagtgatg ttcattgctt caactaaatc agtgattaaa tctttacaga ttttgtaacc
    11041 acccatgtat ttagctgcac ccattgatgt ttggataagt actggtgctt gttttgcttc
    11101 agccgcttca aggattgctt gagtccattc caagttgttt gtgttgaatc cgccgacagc
    11161 gtaaccgcct ttacgagcag cttttaaaaa ttcagctcct gatactactg gcataataaa
    11221 attcctccta agttttgtaa aactaaattt gttaagacaa ttcttaacca acgaccctat
    11281 tttaccaaac atttcggtag ttttctacta aaatcaatga aaatgaaaat tttcaagaag
    11341 ttttttgcaa aaaataaaat atccaatagc gctttaatcg aaaaaagtct tttgctttct
    11401 atcttttttc ctacaaataa taacaaatca gaaaaagaga aaagtgacaa gaaaattgct
    11461 tatccccttc tcttgctact tttctctttc tatcaagttc aaaaattatt ttgtttcgtg
    11521 atctgctaat gatgctccga caaatgcttt gattaatttt tgcgggcgat ttggacgaga
    11581 aatcaattct gggtggaatt ggcatcccac aaagaatttt ttctctggca gctctacgat
    11641 ttctactaaa cggttatctg gtgagacacc tgaaaagact agcccttgtt cttcaaacaa
    11701 ttgacgatat ttgttattga actcatagcg gtgacggtga cgttcttgca caacatcggc
    11761 tccatcatat gcttcagccg tcttcgttcc ttttttgatt ttacatgggt ataatccaag
    11821 gcgtaaagtt ccacccaaat tctcgatgtt ttcctgatca gccattaaat caataatatt
    11881 gtttgctgta tctggattcg tttcagctga tgacgcatct tctaatccaa cgacgttacg
    11941 tgcaaattcg acacaagcca tctgcatacc aagacaaata cctaaaaacg gaacgtcatt
    12001 ttcgcgagca taacgaatcg cttcgatttt cccttctact ccgcgatcac caaatccacc
    12061 aggaaccaag attccatctg cgtctttcaa acgttcttct acattttctc tcgttaattc
    12121 ttgtgctttg atccaatcga tctcgatgtc tgaattatag gcgaaaccag aatgtttcaa
    12181 tgcttcaact actgagatat atgcatctgg taactcgaca tatttaccga caagcgcaat
    12241 ccgtactttt ttcttcaagt tcaagacacg ttcttctaat tctttccatt ctgtcatgtc
    12301 tgcttctggt gtatctagtt ttaaatggtc gcaaacaatt tgatccattc cttgtgcttg
    12361 taaaactaat gggatggagt aaagtgtatc tacatctggc gactcgataa ctgcttctgg
    12421 ttcgacatca caaaattgtg ccaatttgtt tttcacgctt tgagaaacag gttgctctgt
    12481 acgtaccacc aaaatattcg gttggatacc taaaccacgt agctctttta cactgtgctg
    12541 cgttggttta gttttcattt ctccagcagc tttcaagtaa ggaatcaatg ttgtatggat
    12601 atacataaca ttatctgtac cgacatctgc tttcatttga cgaagtgctt caaggaatgg
    12661 caaagattct atatccccta ctgttccccc tacttctgta atgatcacat ctgaatcagt
    12721 cattttagct gctcgcatga ttttttcttt gatctcattt gtaatatgag gaataacttg
    12781 aacagttgct cccaagtatt cgcccttacg ttctttacgc aaaacttcag aatagatttt
    12841 tcctgtcgtc acgttggagt atttgttcaa attgatgtca ataaaacgtt cgtagtggcc
    12901 cagatccaaa tctgtttcag caccatcatc tgttacgaat acttctccgt gctgatatgg
    12961 gctcatcgtc cccggatcca cgttgatgta tggatcaaat ttttggatag ttacttttaa
    13021 gccgcggttt ttcaataagc gtcctaaaga agctgcaaca atcccttttc cgattgatga
    13081 aacgacaccg ccggtcacaa aaatatattt tgtcataagt tcaaaaactc ctttttaatt
    13141 tatgtgcagg aaaagtctca gacaataaac aaagctccct attcttcaat aagaataggg
    13201 agccgaaatt tctgtaaact tctgaccctc taaaagggtg cccaaggtgt ataatacaag
    13261 ctgactaagg gaaagtcaag tactcaaatg aatactatcc gcttacaatt ttttcttttt
    13321 cgatatcctt tgagaaatta tttcttaagt cctttatgag tgcaaaaaaa atttaaccca
    13381 cataataagc ttgatataaa catagctaac gttaaaccta agcaaatcag aaaatcgtta
    13441 ctgaaaaatt ttaagtgaaa taatagataa aaaaataaaa caaacattat gatagataat
    13501 atcccaccta acaaagaact actttttcct acttgcagca acagtttata tagcttatcg
    13561 tagaaacaat ctttttcttt cacccttatt tacctccttt acgtaaaaaa cgtagtccac
    13621 agttctcgga tttttggcat atcttttcta ttaaataata tcaaaaacaa aacaattcca
    13681 ctaatttttt caaagatgac ctttttttca acaaaagtgc cttgtaggga ttcattgaag
    13741 gtaacgaatc acataagtaa aaaataagga gatgcgacaa aagtcgtgcc tccttatttc
    13801 taaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc catcaaacat
    13861 gagaagctgt ttttgaaatt atttcttgtg attcggagtt aaatggtagg ctcacaacct
    13921 ctggataaac ggtattcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg
    13981 tagaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacgtct ttttcaaaac
    14041 ctcttggtaa acggtgttca aaagcttgca tctgcggtaa taagttcagc caattcaaaa
    14101 acaagaaaag ctgtttttgc ttggctattc ttattacttg ttgcagggca gctttttcac
    14161 aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    14221 aaatatgagg aactattttt ccgttagttt ctctaatact caggtcaaaa cgctgatttc
    14281 acagcctcta attcacggta ttcctgtttt aataaagttc tagatattgt tctctttccc
    14341 actcggaaac agtttgtctg aatgccgccc attcgagacg ttttgcttca acaaaatttg
    14401 agaagatatg ttgtcctaat gcgtcaatca ttacattatc cttacgcaat tctttgatag
    14461 cattatggat agttgaaggt aagtcctgga tatgtgcagc ttctctttct tcctcatcca
    14521 ttacataaat gttacggtca acagctgctg gcggtgtgat ctgattacgg ataccgtcta
    14581 gtcctgcttg caacaatact gccattgcca agtatgggtt tgcagatgga tcaactgacc
    14641 gcaattccaa acgtgtggat aaaccgcgag attctggtac acgtactaat ggcgaacgat
    14701 tacggccact ccatgcgaca taaacaggcg cttcataacc tggaacgaga cgtttataag
    14761 aattgactgt tggattgcaa acggctgtgt aggcacgagc atgtttcagc aaaccaccta
    14821 agaaatgata agctgtttca cttagttcca attcaccgtc tttatcatag aaaacatttc
    14881 cttcatcatt gaacaaagac atattgcaat gcataccaga accattgatc ccaaataacg
    14941 gtttcggcat gaaagtcgcg tgaagtccat gcttgcgtgc aatcgttttg acaacgagct
    15001 tgaaggtttg gatattatca caagcatcta ccacatctgc atatttaaag tcaatttcat
    15061 gctgtcctgg cgctacttca tgatgtgaag cttccacttc aaagcctaga ctttctaatt
    15121 ccaacacgat gtcacggcgg caattttcac ctaaatctgt tggtgcaaaa tcaaaataac
    15181 cacctttatc attcaaggtc gttgtgatcc caccgtcttc gtctaattta aataagaaga
    15241 attctggttc aggtcctaaa ttgaaagagg taaaaccaag gtcgcgcata tctttcaatg
    15301 ctcgtttcaa attcccgcga ggatcgccgg caaatggtgt cccatccgga ttatagatat
    15361 cacaaattaa acgagcaact tttccatgag cactttccca cgggaagatc atccaagtgg
    15421 agagatctgg atacaaatac atatcgcttt cttcaattcg aacaaatcct tcaatcgaag
    15481 aaccgtcaaa catcatttta ttttctaata ctttgtcgat ctggctcact ggaacttcta
    15541 catttttgat cgtacctaag atgtctgtga acatcaatcg taaaaatcgt acgttttctt
    15601 cctcaatgat tcgtttgatt tcttcacctg ttatctgttt ctttaccatg gtatccctct
    15661 tcctttttta atttattcag tcaaagaatt gtctttgcaa tccttgcgac tgataatgac
    15721 ctgttttgct taacccacct tgcgccaaaa tctcatcacg aaaaatttgt cgaacgtcat
    15781 gatctgtcaa cggcttcttg ttttttgctt cttgtttttt cgcctgtgcc atttcgtata
    15841 cacgtttgat cccagccata ttcaatccct ctgaaagata gtctttaatc tcaagcaaca
    15901 tatcaatatc attcagcgaa tacatacgtc ggtttccttc gcttcgttcc ggatgaacta
    15961 aatcttgttc ttcataatag cgaatttgcc gagcagacaa atcagtcagc ttcataactg
    16021 taccaattgg aaatacggac atagatcggc gcaattcctt ttctcccatc agacgtcccc
    16081 tttcgtttta taagttggct aaagtgtacc aattaaaaaa aatattgtca actgcatatg
    16141 ttaggttttc taacatgaga tctctatttt ttataaaata acaaaaaaat aagaacacaa
    16201 aaaaggtatc tagttgattt tcgtaccaga taccttttat tttttattat actgtactat
    16261 cctttattag tgtttttatt
//
LOCUS       DACEKG010000063        16075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEKG010000063 DACEKG010000000
VERSION     DACEKG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.066"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="IW664_002012"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="IW664_002012"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="IW664_002013"
     CDS             complement(227..697)
                     /locus_tag="IW664_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0849365.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="IW664_002014"
     CDS             complement(856..1314)
                     /locus_tag="IW664_002014"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0849366.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="IW664_002015"
     CDS             complement(1485..2630)
                     /locus_tag="IW664_002015"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0849367.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="IW664_002016"
     CDS             complement(2643..3560)
                     /locus_tag="IW664_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0849368.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="IW664_002017"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="IW664_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0849369.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="IW664_002018"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="IW664_002018"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0849370.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="IW664_002019"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="IW664_002019"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0849371.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="IW664_002020"
     CDS             7086..7967
                     /locus_tag="IW664_002020"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0849372.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="IW664_002021"
     CDS             7964..8680
                     /locus_tag="IW664_002021"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0849373.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="IW664_002022"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="IW664_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0849374.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="IW664_002023"
     CDS             9397..10398
                     /locus_tag="IW664_002023"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0849375.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="IW664_002024"
     CDS             10395..11756
                     /locus_tag="IW664_002024"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0849376.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="IW664_002025"
     CDS             11716..12867
                     /locus_tag="IW664_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0849377.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="IW664_002026"
     CDS             12842..14011
                     /locus_tag="IW664_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0849378.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..15565
                     /locus_tag="IW664_002027"
     CDS             14186..15565
                     /locus_tag="IW664_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0849379.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15846..16075
                     /locus_tag="IW664_002028"
                     /pseudo
     CDS             15846..16075
                     /locus_tag="IW664_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5257 a         2839 c         3428 g         4551 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacca
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atttgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg ataaaaaaac
    14461 agtaacggtt actagcgcat cagataacga aacaaaagaa tatccttatg acaaattgat
    14521 tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc tagaaaatgt
    14581 ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa atgatccggc
    14641 tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag cagaagcaag
    14701 ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt taggcactta
    14761 tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg gtattgaagt
    14821 tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg caatcaaaac
    14881 aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga aggcaaatac
    14941 agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa caaatgaata
    15001 cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg cttattctat
    15061 cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag aagcacgtta
    15121 tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc
    15181 ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata gctttacagc
    15241 agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac gaccaaaata
    15301 tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg atccaatgac
    15361 ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc aaggaaatgt
    15421 attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag ctgacttctt
    15481 cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc aacaagcgtt
    15541 aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag
    15601 ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    15661 ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca aaaaagggac
    15721 tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg
    15781 tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt tcatgattat
    15841 ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
    15901 ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
    15961 accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa
    16021 aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga aaagt
//
LOCUS       DACEKG010000064        15846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEKG010000064 DACEKG010000000
VERSION     DACEKG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.067"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(286..486)
                     /locus_tag="IW664_002029"
     CDS             complement(286..486)
                     /locus_tag="IW664_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0849380.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(791..2023)
                     /locus_tag="IW664_002030"
     CDS             complement(791..2023)
                     /locus_tag="IW664_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0849381.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2280..2849
                     /locus_tag="IW664_002031"
     CDS             2280..2849
                     /locus_tag="IW664_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0849382.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3027..3467)
                     /locus_tag="IW664_002032"
     CDS             complement(3027..3467)
                     /locus_tag="IW664_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0849383.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3625..4389
                     /gene="map"
                     /locus_tag="IW664_002033"
     CDS             3625..4389
                     /gene="map"
                     /locus_tag="IW664_002033"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0849384.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4421..5344
                     /locus_tag="IW664_002034"
     CDS             4421..5344
                     /locus_tag="IW664_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0849385.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5420..6559
                     /locus_tag="IW664_002035"
     CDS             5420..6559
                     /locus_tag="IW664_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0849386.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6552..7352
                     /locus_tag="IW664_002036"
     CDS             6552..7352
                     /locus_tag="IW664_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0849387.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7352..8179
                     /locus_tag="IW664_002037"
     CDS             7352..8179
                     /locus_tag="IW664_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0849388.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8157..8891
                     /locus_tag="IW664_002038"
     CDS             8157..8891
                     /locus_tag="IW664_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0849389.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8991..9857
                     /gene="rfbA"
                     /locus_tag="IW664_002039"
     CDS             8991..9857
                     /gene="rfbA"
                     /locus_tag="IW664_002039"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0849390.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9871..10443
                     /gene="rfbC"
                     /locus_tag="IW664_002040"
     CDS             9871..10443
                     /gene="rfbC"
                     /locus_tag="IW664_002040"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0849391.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10465..11493
                     /gene="rfbB"
                     /locus_tag="IW664_002041"
     CDS             10465..11493
                     /gene="rfbB"
                     /locus_tag="IW664_002041"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0849392.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11591..12442
                     /gene="rfbD"
                     /locus_tag="IW664_002042"
     CDS             11591..12442
                     /gene="rfbD"
                     /locus_tag="IW664_002042"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0849393.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12476..14509
                     /locus_tag="IW664_002043"
     CDS             12476..14509
                     /locus_tag="IW664_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849394.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14553..15833
                     /locus_tag="IW664_002044"
     CDS             14553..15833
                     /locus_tag="IW664_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0849395.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         4867 a         2734 c         3285 g         4960 t
ORIGIN      
        1 atttaaacca taattgtgtt tttccttcca tcagcataaa agttcacctc aaaaagagta
       61 tacgaacgaa tgttcttttt gtgaaggcgg aatttaatat actgaataat agaaaacaag
      121 tacttgtgcc aaattatgtg ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa
      181 aaccttctat attaagattt cctgcaataa caaacacaac agaagacgtg taatagttag
      241 tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt
      301 agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac
      361 tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccctcaataa acagtcgaag
      421 agaaaatagg caaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat
      481 tttcatactt caacagattc gaattaaacg atttcgattg gcttatacca gtcctatccg
      541 acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat
      601 gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta
      661 caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc gaaataaagg
      721 atctaagcca atagcttaga tcctttattc tatgctttat attatttttt gcatgtgtgc
      781 gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag ccatcttcac
      841 ggattccgtc gatatccatc ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc
      901 tattcaatcc agcttctgct aattcttcat cggacatttc tgtaccgcct ttcacactga
      961 aggcataagc agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga
     1021 aaaagatgat gccagattga gaaataggtg aagggtcagg tacaagtgcg acttctccta
     1081 aacgacgcgc accttcatct gtatcaagca gttttgctaa gacatcttct ccttgttcgg
     1141 cagaaaaatc aatgactttt ccatctttaa aagtaaattt catacccgag atgatggtgc
     1201 cagcataact cagtggcttt gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg
     1261 ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt ttctccacga gcattatagc
     1321 ttccagcgcc ttcccaaaga tggtttttag gcaatccgat aatgatatct gtaccgggag
     1381 cagtatagtg cagggcagaa aattgttctc gattcaattc ttctgctttt tttgctaatt
     1441 tttcatcatg cttcttccat gctaagacag ggtcttcttc atagacacga gtcgttttga
     1501 aaatctgatc ccataatgcc tcaacttgtt tttcttctgg cagttctggg aagacttttg
     1561 ctgcccattg tttaccagcc gcagccacga cagtccaact gactttattt gcttgagttg
     1621 ccttgcgtaa attcattagt gctttgccag ccgctgattg ataagacgct actcgatggc
     1681 tatctacacc agcaaatgca tcgggatcgg cagaaacaac actgatcctg cttgctcctt
     1741 tttcaagcca ttcatccgct tgatcgattt tagattgtgg tacattctca atacgatccg
     1801 ttgcagcatg taaaagaaat tctctttgga tctggtcgtc agtccattga acgataacct
     1861 cagcggctcc tagtttataa gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca
     1921 cgctgatctg taaaacaact gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta
     1981 agcgtgcata tttttttaga agttcattaa aatcagataa cataataaaa attcctcctc
     2041 accatttttt ctggtaacta ttagcatgat accatttaat tatgattcaa acaatgaggc
     2101 ctttaaataa aaaaaagagg ttattttttt aaggttaagt ttcatgatat atgttactac
     2161 ttgctatttc agcttgtgaa gaaaatcaaa aactaatttt gcatatcaaa aaaaacatgt
     2221 tacaatgaaa ttaatcaatg gtggaagcgt tataaaaaaa ttaaggagat taaaaaaata
     2281 tggcaagaaa aaagacgatt actagagaac agattttggc agctgcttat gaagtagctg
     2341 ccaccgatgg atttacacga tttactgcga gaaacatcgc gaacaaaatg aattgttcca
     2401 cgcaaccgat ttatcttgag tttaaaaata tggatgactt gagacaagct ctttttgagc
     2461 agatctatga tcatttacaa tatgaagtgt tcactgttga gcatacaggg aatacgatta
     2521 tcgatcttgc ttttaattat atccattttg caagaagaga gaagaagttg tatcgttcac
     2581 tgtacttaga agaatatggt ggaggaaaag agatgcagaa tttctcttat acttactttg
     2641 tgaatacagt gaaacgcgat ccagcttatg tgaatctttc agaagagcag atcgaatctt
     2701 tgtataatgg gacatggatc atagctactg ggatcgcagc tttgatgtct tcaagcatca
     2761 tccatccaac tgatgaacag attgaaaaga tgatacaaga cagtatagat gctatcttga
     2821 aattcaaaga accaatagaa atcgattgaa tatactcaaa agttccacaa atcagtagat
     2881 aacaagagat gtgtcaaaat aaggaaaagt tcgtttttct tacagcgtat gcatttttaa
     2941 ctcccgaaga actcttccat gaatgcaatg aataaaagag ccgtacagat tgaataaatc
     3001 tggatggctc ttttattgca agtatattaa atttttgcga ctagttcttt agcgaaagct
     3061 tctaaatttt cgatatcttc ttcttcagca ttcagatcca ctttaacatt ttcggctcct
     3121 tttgatgcgc ctgtttttgc aaaagcagca tcgaaatcat ccaccgattt gcagaattca
     3181 tcgtaaaaag tatcaccaga tccgcagaca ccgtaaattt ttcccgatag atccaattct
     3241 tgcaagtctt cataaaagtc tacgatctca tcaggcagat cgccatcatc gtatgtataa
     3301 gtagctacta cgcaaatatc tgcctcctcg aattcatctg catctacttg ggtacattcg
     3361 ttgatttcga cttcgatatc cagattttca aatgcttctg caacaatatc ggcgatttct
     3421 tcagtgtttc ctgtcatgct agcatatacg atttttgcta aagccatgat gtgcctcctc
     3481 gtttataatt atttaacaat tatgaattat accaaaacat cattgaaaag aaaataaaac
     3541 ctagagacac ctggggaatg tcatttggct aaatgtatgc cgctatgtta aaatgagagg
     3601 gatttgtaaa aaggagacga aaatatgatt actttaaaat cagcacgtga aattgaaatg
     3661 atggcagaat ctggagcttt attagcagat gtacataaaa atctgcgtga ttttataaaa
     3721 ccaggaatca caagttggga tattgaggta tttgtcagaa actatatcga gagtcatggc
     3781 ggaattgctg cgcaaattgg ctttgaaggg tatgaatatg cgacttgtat cagtataaat
     3841 gatgaaattt gtcatggatt ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa
     3901 gtcgatatgt gtatcgattt gaaaggcggc atttctgatt cttgttgggc ctatgtagtt
     3961 ggagaatcta cacctgagat cgaccgattg atggaagtaa cgaagaaagc tttgtacatc
     4021 ggtattgagc aagcacaagt agggaatcgg attggtgaca tcggtcatgc tatccaaact
     4081 tatgtagaag gcgaaaatct agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct
     4141 acgattcatg aagaaccagc agttcctcat tacggagaag ctggaaaagg gttgcggttg
     4201 aaagaaggca tggttatcac gatcgaacct atggttaata ccggaacttg gaaaatgaaa
     4261 atggatccta acggctggac tgcgtataca agagacggcg gactttcttg ccaatatgaa
     4321 catacattgg ctattactaa agatggtcca agaattttga catcacaagg agaagaaggc
     4381 acttattgat ctatccagtc aataggtacg ggagaggtac atgaaagttt ggaacaaaat
     4441 aaaacaaaat gaaaatctga tgcgctttat tgagacgact caaaagcgca tcactgattc
     4501 agaaatgagt acgacctctg tcgtcgttgc ttattattta ctcttgtctt tatttccatt
     4561 gattattgct tttggtaata ttttaccgtt tctccacatc gatcaagaga ctgtactgac
     4621 ctatttacga gaagtgattc ctgagaccgt ttaccagttt atcggtcctg caatcaaaga
     4681 ccttttgaca caaagttcag gaggcttgtt atccgtttct gcattagcgg ctttatggtc
     4741 tgcaagtcaa agtatcaatg ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa
     4801 tagaaaaaat tttttgatcg tccgtttctt ctcactaatt gtcattgtct tgttcatgat
     4861 tgctattagt ggggtaacta tcgttcttgg gttgggacag ctgattttgg atgctattca
     4921 gccaatcgta caaatacccg tgaactttat cgatcagttc caaactttga agtggccaat
     4981 cacattggta gctttatttg tcatcatgtt cttgatttat ttaattgtcc ctaatgctca
     5041 gttgaagttg aagacgatca ttccgggcac gatcttcgct actgtcggct ggatgctgtt
     5101 atcacaagtg tttagtatct atgctaggta ttttgcaacg aggatcagcg gttatcagat
     5161 cataggaagt tttattgtac tcatgctttg gcttaatttt gctgctacga tcatcatttt
     5221 gggcggaatc atcaatgcag ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg
     5281 tcgagatcca agccgaaaat ggttcaataa attgaaaact aaattttctc ataaaaaaaa
     5341 gtagaaacaa gagttcttat tgtaaattta tatcagtaat ttataataat tatgactctt
     5401 aattaaagta ggtgcccata tgcttgtttc ttttagtatg gttataagat tttttctgac
     5461 aattttatta tcactgatat taacacctgt attcaaaatc atttcagtac aaacaggtat
     5521 ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca atgccttcag cgggcggact
     5581 gcctatcttt atttcatttg ttatatcgac tttattctta ttccgtaaaa ttatccctca
     5641 atcgtatatt gtaccgatct tgattgcttc tttagtaatc atagtcacag gagtgctcga
     5701 tgataaatat gagctgacac ctaaacaaaa aagtataggg atcttgattg cttctcttat
     5761 tatttatttt gtagcagata tacgtattga ttccttcacg cttcctttta ttggatatat
     5821 ccaactaggc tggcttagtt ttcccgtaac gattttttgg atcttcggta tcacgaatgc
     5881 ggtcaatctg attgacggtt tggatgggct ggcggcaggg atttcattga tcggtttaat
     5941 tacgatagga atcattggat attttttcct ccatgcttct acagtgtata ttccagttgt
     6001 tattttctgt cttgtagcaa gtattatcgg tttttttcct tacaattttt atccggcaaa
     6061 gatttattta ggcgatacag gagctctatt cctagggttt atgatggccg tcctttcact
     6121 acaagggctg aagaacgtga cttttgtttc atcgatttct ttgctgatcg tcatgggagt
     6181 gccggtaaca gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg tttctttttc
     6241 tacagcagat aaaaaacatc ttcatcaccg actaatcagt ttaggcttta ctcataaagg
     6301 tgctgtcctg acgatttatg cgatggcttt gatgttttct ttcacagcta tggtcatgaa
     6361 ttatactgga agaatcggaa cgatcatatt gattattgcc atgttgtttg ctgcaatttt
     6421 attatttgaa ttgattggtc tgatcaatga gaaacatcag tttattttaa aaacattgcg
     6481 atttttaggg aataaagagt accgtacgca agtaaaacag aaatatttga agaaatggaa
     6541 ccgtcatata gatgaataac aaattaaacc agccgatcct tattagtatt gtgacggcaa
     6601 acagtgataa aatttttcgg acactagata attttatttc aactgtaaaa gatgaaacag
     6661 cagtaaaaat ccgaattttt gacaacaatt cacttcccga gtacaaaaaa agactaacag
     6721 agtataggaa atatccctac atcgatatct attttcatga tgaaaattcc ggatttggtt
     6781 atggacataa tcacaatctg atagctagcg attctcgcta tggcattatt tgtaacccag
     6841 atattttagt ggatgaagag acagttacag cattggtctt gttattgaaa gaacatccgg
     6901 aatgtgcgat gttagcacct aagattctta atgaagatgg aacaactcag cacctcatcc
     6961 gtgagcagct ggctgttttt gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa
     7021 aaaagtttga caaacgactg gctgtgtttg aatgccgagg attgcctact gatgctaata
     7081 gttatgtacg gatgatctca ggtagtttta tggtagctga tattgataaa tttaaagaga
     7141 taaaggggtt tgatgagcgt ttcttcatgt actttgaaga caacgacttg tgtttgagtt
     7201 ttgaaaaagc aggttacaag ttattgtaca caccctttta tgcagttatc catttttacg
     7261 ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat
     7321 ttttcaataa gtgggggtgg cgttttttct gatgaaacca gaaatattag tagtcgttgt
     7381 gctttatcag caactattca gtcaatctcc agcttatgga ccattaagca aagcacttag
     7441 cgaaaagcaa attcagctta ttatttacga taatagtcct caaaggcagc aaaatgagct
     7501 gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa aatcctggtt tagcggtggc
     7561 ttataatttt gcgttgtctc aagcagatga aggaacacgt tgtctcgtca ctctggatca
     7621 ggatacgagg ctagtcgacg attattttga actattgcgt aaagtaacct ttacagatga
     7681 atgtgttgct gcagttccga tgattttttc tgggagccga caagtttctc ccctatattc
     7741 agatcgttat atcaatcgta cagctcaagc agtagaagtt gaaggcccaa cagctgaacg
     7801 aatcatggca atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg
     7861 gtttaatcct gctttttctt tagatttttt ggatcattgg ctgttttgga aagtggatca
     7921 gttggaaaaa actgtggaga tccttcctgt acgaatggaa catgatcttt cggttttaga
     7981 ctatgaaaaa gtttcaagta atcgataccg ttctatttta caagcagaaa atcgatttta
     8041 tcatgaatat gatcaaaggc atttatccag tcaccgacga caattattgt taagaacagc
     8101 taaacagttc ttgaccgtca aaaaccggca tatctggcgg atgactttgc gctcatatgt
     8161 taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt aatggaggca
     8221 gatatttaag agagcagtta gatagtattt tatctcagct ttcagcagaa gatgaagtag
     8281 tcatttctga tgatgggtca gtagatgata catgggatat attaactgaa tacgcagaaa
     8341 aagataagcg aatccagctt ttttcggggc ctcgtcaagg attgatcgct aattttggtt
     8401 atgcgatcca gcagacgaaa ggagatctaa tctttttggc agaccaagat gatgtttggt
     8461 tggagacaaa agtgaaaaag atcactgact attttgagca gaatccggat cagctagtag
     8521 tagtttctga tttaatcatt gtggacagcg agctgcaaga gatccatgat tcgtattttt
     8581 cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat aagaagtcac tatattggtg
     8641 ctggtatggc tttccggtct tctttaaaac ccgatatttt gccgatcccg tcagaagtgc
     8701 cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa gactgggttc ctaagagaac
     8761 cgttaactct gtaccgaaga catgatttaa acgcgagtga gattgaaaca cattctaaat
     8821 tacggcaaaa aatttcttgg cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt
     8881 ttaaacgttg ataaaagatg tttaaatcct taatatttcc atgacaaata taacaattga
     8941 tgagataata acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc atgaagggaa
     9001 tcattcttgc tgggggcagc ggaacacgtc tgtacccttt aacaaaggca acatcgaaac
     9061 aattgatgcc gatttatgac aaaccaatga tttattatcc aatgtctact ctgatgttgg
     9121 cgggaatcaa agaaattctg attatttcaa cgccacaaga tacaccacgt ttcaaggaat
     9181 tatttggaaa cggaaatgat ttaggactgc agatcgaata tgcagtacaa gaaagtccag
     9241 atggattggc acaagcgttt atcattggag aagaatttat tggagatgat agtgtctgct
     9301 tggtattagg ggacaatatt tactatggtg gcggtctgtc taaaatgttg caacgagcag
     9361 cttcaaaaga tacaggcgcg acagtatttg gctatcatgt caatgatcca gaacgctttg
     9421 gtgtagtaga atttgatgag aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc
     9481 caaaatcaaa ttacgctgta actggccttt atttttatga caatgaggta atttctatcg
     9541 ctaaaggtat caaaccttca gaacgtggag aactagagat tacggatgtc aataaagcat
     9601 atttggataa aggaaaacta tccgtagaac ttatgggacg cggatttgct tggcttgata
     9661 caggaacaca tgaatcattg cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc
     9721 agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg tatgggctac atcgataagg
     9781 aacaattgct ggcccttgca cagccattga aaaaaaatca atacggtcaa tatttattga
     9841 atttggccgc tgaataagga gtccttacag atgaaagtaa cagatacaaa attaacagat
     9901 gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg gtttcttcac agaaagttat
     9961 tcaaaagcaa aatttgcaga acatggattg aaatatgact ttgttcaaga caaccattca
    10021 ctatcagctg aagctggtgt ccttcggggg cttcatttcc aaaaaggaga agctgcacag
    10081 acaaaattga ttcgggtagt cactggagca gttttagatg tagtagtgga tatccgcaaa
    10141 ggtagcccga cttatggtca atgggacgga tttatcttat ctgaacataa tcatcgacag
    10201 ttgcttgtac ctaaaggata tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc
    10261 ctttataagt gtgataatta ctataatgca gaagcagatg ggggcatcgc atttgatgat
    10321 ccagatttag cgattgattg gccaattgat ccatcaagag cgattatgtc ggaaaaagat
    10381 caccgtcatc cgacattaaa agaatttgaa gcagaaaatc catttgttta cggagaaatc
    10441 taagaattaa ggagaaattt tcttatgaaa aacatcattg ttaccggagg tgccggattc
    10501 atcggctcta atttcgttca ttacgtggta aacaatcatc cagaagtgca tgtcactgtt
    10561 ttggataaac tgacttatgc aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc
    10621 gtagaattgg tcgtaggaga tgttgcagat gcagaattag tggaccgtct agtaaaagaa
    10681 gcggacgcag ttgttcatta cgcggcagaa tcccataacg ataattcatt aaaagatcct
    10741 ttcccatttg tacaaacgaa tatcattggt acgtatacac ttttagaagc ttgccgcaag
    10801 tatgatgtac gctatcatca cgtatctact gacgaggttt acggcgatct tccattacgt
    10861 gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat
    10921 aatccttcaa gtccatattc ttctacaaaa gctggttcag atctgttagt aaaagcatgg
    10981 gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt caaacaacta tggtccttat
    11041 cagcatattg aaaaatttat tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca
    11101 aaattatatg gcgaaggaaa gaatgttcgt gactggatcc atacgaatga tcattcttca
    11161 gcagtgtggc tgattttgac gaaaggcaga attggcgaaa cctatctgat cggtgcagac
    11221 ggtgaagaag ataataagac cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa
    11281 gatgcgtacg accatgtaaa cgaccgtgca ggacacgact tgcgttatgc aatcgattcg
    11341 acaaaattac gggaagagtt aggatggaag cctgagttta cgaacttccg tgatgggttg
    11401 gctgacacga tcaaatggta cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc
    11461 gttgaagcag catatgccaa aaatggacaa taatacagag aaaatgtcac tgtattaatg
    11521 gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa atcaaggagc
    11581 ggataatcat atgtttttat taactggagg aaatgggcaa ctaggtacag aattgcgcca
    11641 tttattagat gaaaaaaatc tgcaatatgt ttcaacagat gcaaaagact tggatatcac
    11701 agatgccgag aaaacaatgg cttacatcac agaattaaaa cctgatgtta ttttccattg
    11761 tgcagcttac acagcagtag ataaagcaga agaagaagca aaagaattag atgagaagat
    11821 caatgttgat ggtacaagaa atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt
    11881 ctatatcagt acagactatg ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga
    11941 cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt
    12001 agaagagatt ttagaagact attatatcat acgaacttct tgggttttcg ggatctatgg
    12061 acacaatttc gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt
    12121 aaatgaccag tttgggcgac caacttggac acgtactttg gcagaattta tggtatatgt
    12181 catcgaagaa aaagcaccat ttggtattta tcatttgtcg aacgaaaata gctgttcttg
    12241 gtatgaattc gcaaaagaaa tattgaaaga tacagatgta gaagttgcac ctgttacgtc
    12301 agaagaattt ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc tgaaaaagac
    12361 agaagagtta ggatttgtca taccaacttg gcaagaagca cttgcgcaaa tgctggaagc
    12421 tgccaaaaaa atgaataagt aatttccttt tatcaaatag aatttggagc atgatatgga
    12481 aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa
    12541 tttggtgctt gacctgtttc taaaactccg ctatcttatt ggcgttttag tcatcgtggt
    12601 catcgttact tttaatttaa acggaagttc acttggtgtt tgggataaat atgtgtcgca
    12661 aagagacgat gggaaaaaaa ctgatgtgat ttttggacag aatcgggagg tgcggtcaga
    12721 cgaatggctg gtgcagacac ctttttacct ctcacaagct gaagaggact ttccgttggt
    12781 gaacgaggat tattcattaa acggacaaaa catgatcatt gcgtataatt ctcctgcaaa
    12841 ggatattacg gttatcggaa aaccattcaa ctggggattc tttttcctcg gaagagatcg
    12901 aggactttcc ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt
    12961 actgatgata ttaacgaaga gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac
    13021 attttctcca gcagtccagt ggtggttcat gcaacatgtg ggcgatttga ttttctttac
    13081 tttgggactg atgactgctt tttatcatta tttctacaat catgataaaa aatggctacg
    13141 aacgttgatg atgcttttgg tggttatttt cggtattggg tttattttag tgatttaccc
    13201 agcccatcaa gtgatgctgg cgtacttgct gattttttat tttgttggcc tgcttattta
    13261 ctatagcaag aaagtttcat gggatctgtt ggatgtagtg ttgattggag gggcagtttt
    13321 atttattggc ggagtcatgg ttcatttctg gctaacgtca aaagacgcat tgttagcttc
    13381 tttgaatacc ttatatccag gaaaacgggt aagtactgga ggaaagtgga cgattggcga
    13441 atttttttct ttccttacaa attggaaaat tccttttgaa gatatcaact attcgaataa
    13501 ctctgaagtg gccctgttct atcacttttt tccaactgta ttcctagcaa gtccatttgt
    13561 gttatttggc aaaaaagata gcgaacaaaa gttactggga cgtattttga tgattttttg
    13621 tctgttttct atcttttgga ttaccgttgg gttgccaaaa tggctagcag aaatcactct
    13681 gctgtcttat gttcctccat taagagccat ccttactttt agttttgcgg cttgtttatt
    13741 atcgatctgg tttatcgctt atatttggag agaagatgtt attcctattt ggtataaatt
    13801 cattttgttg gcagtaaatg catggtctta tttctatgcg attacgcatt cgaaaataga
    13861 taattatttt tcggattttg aaaccatcgc agttgctgtg ttgggaacag tgattttagc
    13921 atttgcatta tttggctgga gacgcttgtt ttacctcgct tttgccgcct tagttatcgt
    13981 atctggcttt ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg aaaaaacact
    14041 ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt aaagtttggt tgtctgaaga
    14101 tgaattatat aacttcaccc caacattggg agtgaaatcc ttcaatacag tacgctttta
    14161 tccagatata gctgcatgga aggaaatcga tccgaaagaa gaatacgaga agtattataa
    14221 tcggtatgct catacaagag cttttatcgc taaggaagat acaaagttca ttttagatcg
    14281 tccagatatg ttttcggtca cgttgaattt tgaagacgca gaaaaattag ggatcgacta
    14341 tgtgatcagt aaacgaccat taactgatta caaccaatta tatgaagcgc agttcacgca
    14401 gctatatgga ccggataaag acggttatat gatttttaaa gtagaatatc cggattatcc
    14461 agatgtacca caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat aggctagctg
    14521 agaataatcc tgaaatatta gtagaaagga gagtggagat aattcaagca atcgtacaaa
    14581 aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt gctgacgcta gtcttttttg
    14641 tttggacagc agggttaggt tttccagcaa ctttgtttat atataacaat agttcattac
    14701 ttatcgcttt tagtgtggct attttgctca ttttaaatgt ttataaagca aaaggcgtcg
    14761 attttcttta tctaggtgtt gcgcttgccc ttttcatttt cttttcttac tttcgggaat
    14821 taagaaatga gtctttattt ggagatttac tgtttccttt gattatttta ttttgtttag
    14881 taattaaatg gatcgagtgg acaaaatggg atcgcggtat ttatctgatt gtctttacgg
    14941 tcttcctcgg aatcacgtta tatcgtgtat ttacagagat ccaagtacca gaaggacaaa
    15001 gcatctgggc tccaggcaat tcgctttctg atatttggat caatacgaat acaatcggaa
    15061 gttctttgat gactcttgct ttattgatca gtggatttgc cagttctttt gaacgatggt
    15121 atgtacgaat catcggtgca gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc
    15181 aatctagagg tgcattagtc gcgttgattg tttttgcgat attggatctt ttacctaaat
    15241 cgttgatgcg tgtcattcgt gcgccattta ttgcttacac ggttacgatt cttttagctt
    15301 tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag
    15361 atatttggca taaattctat cagacgctag gagaaaaatc tgaacaagtt ttactaggaa
    15421 tgaaaacatt tatcttccaa cgaggaaacc aatttttagg aaaccacaat agttataatt
    15481 ctattttgaa tatctatgga ttgataggat ttgggattgc tactttgctg cttatccttt
    15541 ttattggtcg cttaacgttg aaagcagatt tatccaacgg gcagatgact tttatctggg
    15601 ccttttttgc ggtgatgatg cagtcattta tggaggatac attgacttca tacccatggg
    15661 ttccgattat ttatatcctg ttggctatgg ccgttcatcg atatgatgaa ccagaagttg
    15721 cggaggaatt ctatgttatg gaagaggcat atgacgatat gacttatgat gatacatctt
    15781 atggggaaca accagatagc gctcattctt ccaggatcca acgctatcat tgataggcac
    15841 aaaata
//
LOCUS       DACEKG010000065        15687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEKG010000065 DACEKG010000000
VERSION     DACEKG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.068"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..751
                     /locus_tag="IW664_002045"
     CDS             68..751
                     /locus_tag="IW664_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0849396.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IW664_002046"
     CDS             753..1589
                     /locus_tag="IW664_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0849397.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IW664_002047"
     CDS             1600..3354
                     /locus_tag="IW664_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849398.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="IW664_002048"
     CDS             complement(3630..3971)
                     /locus_tag="IW664_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0849399.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="IW664_002049"
     CDS             complement(4039..6210)
                     /locus_tag="IW664_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0849400.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="IW664_002050"
     CDS             6421..7278
                     /locus_tag="IW664_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0849401.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="IW664_002051"
     CDS             complement(7349..8206)
                     /locus_tag="IW664_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0849402.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="IW664_002052"
     CDS             complement(8191..10893)
                     /locus_tag="IW664_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0849403.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="IW664_002053"
     CDS             complement(10906..12195)
                     /locus_tag="IW664_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0849404.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="IW664_002054"
     CDS             12345..13391
                     /locus_tag="IW664_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0849405.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="IW664_002055"
     CDS             13393..15546
                     /locus_tag="IW664_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0849406.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4455 a         3282 c         2886 g         5064 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacaa agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DACEKG010000066        15514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEKG010000066 DACEKG010000000
VERSION     DACEKG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.069"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(331..633)
                     /locus_tag="IW664_002056"
     CDS             complement(331..633)
                     /locus_tag="IW664_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849407.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(853..1219)
                     /gene="ssrA"
                     /locus_tag="IW664_002057"
     tmRNA           complement(853..1219)
                     /gene="ssrA"
                     /locus_tag="IW664_002057"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1390..2163)
                     /locus_tag="IW664_002058"
     CDS             complement(1390..2163)
                     /locus_tag="IW664_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0849408.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2307..2657
                     /locus_tag="IW664_002059"
     CDS             2307..2657
                     /locus_tag="IW664_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0849409.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2638..3354
                     /locus_tag="IW664_002060"
     CDS             2638..3354
                     /locus_tag="IW664_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0849410.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3354..4802
                     /locus_tag="IW664_002061"
     CDS             3354..4802
                     /locus_tag="IW664_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0849411.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4872..5381)
                     /locus_tag="IW664_002062"
     CDS             complement(4872..5381)
                     /locus_tag="IW664_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849412.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5371..6096)
                     /locus_tag="IW664_002063"
     CDS             complement(5371..6096)
                     /locus_tag="IW664_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849413.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6319..8439)
                     /locus_tag="IW664_002064"
     CDS             complement(6319..8439)
                     /locus_tag="IW664_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0849414.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8669..9847
                     /locus_tag="IW664_002065"
     CDS             8669..9847
                     /locus_tag="IW664_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0849415.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9863..10621
                     /gene="aroD"
                     /locus_tag="IW664_002066"
     CDS             9863..10621
                     /gene="aroD"
                     /locus_tag="IW664_002066"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0849416.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10644..11129
                     /gene="ybaK"
                     /locus_tag="IW664_002067"
     CDS             10644..11129
                     /gene="ybaK"
                     /locus_tag="IW664_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0849417.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11200..12453)
                     /locus_tag="IW664_002068"
     CDS             complement(11200..12453)
                     /locus_tag="IW664_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0849418.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12570..13919
                     /gene="gdhA"
                     /locus_tag="IW664_002069"
     CDS             12570..13919
                     /gene="gdhA"
                     /locus_tag="IW664_002069"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0849419.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14031..15377
                     /locus_tag="IW664_002070"
     CDS             14031..15377
                     /locus_tag="IW664_002070"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0849420.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5126 a         2750 c         3125 g         4513 t
ORIGIN      
        1 gaaagacatt tgggttatat tgtgagataa taataaagaa gagtttaaag cgttccccaa
       61 aaaccacttc cccataagtg tgttacgctt taaactcttt tatatttgaa gccattaaaa
      121 agcataccat ataactgtaa aaaataatgg gaaaaagact tataattgga gtggtagtta
      181 attagtgact tatttttgat tttatagcac tgatactata aaatatagat atcatcatat
      241 tacacaatct taatactaac ttaaaaaata tcccttttat aagtatggtg ataaaatccg
      301 ttccgggcta ccttttttag gtagcctact ttaatcttta tacctttctg gatcaacgaa
      361 agtatacttc aaatagtcat aacgccgatg atcgctccgt gcgtctggca catcagtcac
      421 gatatcaaac aaaaaataca catctttctt cattctggtt tttgttgccg gaattttaaa
      481 gtagttctta ttagagtaat agagattgat taataagcta tcttcgattg ctaaaaagaa
      541 aacttctgaa tcccacacct tataaaaatc tttgacaaat ctattcgaag gatcaaattt
      601 aaaccataat tgtgtttttc cttccatcaa cataatgttc acctcaaaaa gagtatacga
      661 actaatgttc tttttgtaaa gtaggaatat cattgataat atggatggaa catatatatt
      721 aaaaagtgtt ctttttgaat gctattttga atgccatttt cattcattta acgaaaatct
      781 gcgaaaataa aaaatgctca aacccttata aaataaggat ttaagcatct agcgaaatct
      841 gctgaaatct cacaatggag acggcgggag tcgaacccgc gtccaaacac atcgccactt
      901 aaaaatctac gttcatagtc aactcattta aggattcact ttacagcttg ccgagtgaca
      961 ggcattctgt atcgctagtc tgatgttctc tttttcaact tacagacgga aagttgaaac
     1021 gtatcccact aaagttagga cccttactcg agcacatggg cgatgccgag aggatctacg
     1081 ctaactgttt ttaggcagct aaagcgtaag agttgttttc gtttttagca gttatattta
     1141 actgtaacgt ttttacgtag acgtaaccta cgaaacgcaa ttcaagctcg actgtgcctg
     1201 tcgaatccgt aacgtccccg ttagctaaca agggttacgt gtatttttca tacaaaatta
     1261 gtatagcata tattgggata aattcaataa aaaagcgaat catatcccat tatttatata
     1321 ataactgccc ctttcttaag aaaaaataag cgattttctt aagaaaagag gtagcttcaa
     1381 aaaaagatct taaatcagat caaaatactt caatgcgtgg acaattccgc cttctgtatt
     1441 tttctttgtg ataaaggtag aaagttcttt taatccgtca taggcgttgc ccatagcgat
     1501 tttgttgtcg caagcttcca acaaggcaaa atcattcgga ccatcgccaa atccaaaagt
     1561 aggaactttt tccagctgaa gttctttttg cagacgtttg acacctgccc cttttgagat
     1621 tcctttcttc actgtatcga tcgagtaagg accattccga taaaaagtta gctcaggaaa
     1681 atgttcgtga tagtattcgt cacctgattg agataagatc agcaacatat ttactgtgtt
     1741 ttctttaaag cctaaaggat caatttccgg aacatctgaa tgaatgaagt cgtaagcatt
     1801 gatcattgtt tggctatgac cggtacacca gattttctga tcgttatagt aagaaagttc
     1861 atgaccgttc aactgtacat gatcatacat tttttgacat tcttctgaag aaaatgtttg
     1921 agaatagatt tcttcgccgt ctactcgaat gaaagaacca ttcatggtga ccgcagaagt
     1981 aatgcccgca tcttccatga tagagtggat ttctgcttct gtacgtcctg tagcgatgac
     2041 ggggagaaca ttgttttctt ttaatgcgtt catagctttt gccacttcgg gtgtgatttt
     2101 tgatttcgca tctagtaatg ttccatcaag atcaaagaaa gtgattgcac gataatttga
     2161 cacaaataaa actccttttt tattggaata agtttatctt aacaaagaat gaacaaaaga
     2221 caaacctttc gtgaagaatt cctgaacata ttggttcagt caacaattta gagtatacta
     2281 taagaagaga atttaggagg gaaagaatga tttttttcag tattggtatc ttattcttaa
     2341 taattggcag tctttttcgg attctgccct caaaaggaaa tctgcctttt tatgggtatc
     2401 attcgcctct tgccgcaaaa acagacgctc attggcggct ggctcaaaag acaagcggaa
     2461 actggttttt tcttatgggc ttactgatgg cattgatcgg ctattattta aagacatctg
     2521 ggcatacgaa ttattttctg attgaaatgc ttctgctagt atttccaatt atgcctattt
     2581 ttattatgac agaaaagaaa ttacagaaat atgacctgga aactggaggg aacgataatg
     2641 aatattttaa tgattgaaga taatcaatct gtttctgaaa tgatgcagat gtttttctta
     2701 aatgaaggtt gggaagcaac tttcaagtat gatggaaaag aagggctcga tgccttttta
     2761 gaaaacccac aacattggga tatgattaca ctagacctga atttgcccac actggacggt
     2821 atgctagtag gaagagagat ccgcaaagtt tcaagtactg tgccgattat catgctgaca
     2881 gctagagatt ctgaaagcga tcaggtgatc ggcttagaga tgggggcaga tgattatgtc
     2941 acaaaacctt tcagtcctct gacgcttatt gctcgaatga aagctttgca tcgccgtagt
     3001 gagttggcag aagcaagaga agaagcagta gtttcagatg atcattttga tatcgagact
     3061 agtcatttca agatgaatac gaaaacacga gaagcgtatc tggacaaccg gccaattgaa
     3121 ggactgacgc ctaaagagtt cgatctattg tttactttag caaaaaaacc acgtcaagta
     3181 ttctctcggg agcagttgct tgaattagtc tgggattatc agtactttgg tgatgaacgg
     3241 acagtggacg cccatatcaa gaaattacga caaaaaatcg aaaaggtcgg accacaagtc
     3301 atccaaactg tttggggagt cggctacaaa ttcgatgatt ctggtgttgc ctaatgcgtt
     3361 atttgtatca gcaactctta gctttctgcg gaatggtcgc gttgatctta ttgatcgttg
     3421 gagtttcttt tacacaactg acaaaacaaa cgattgaaga aaacaattac cagcaactgt
     3481 ttggatatgc tgaatcagtt gaaaagacaa cgcaaaccta tgccgatacg ctcccgcagt
     3541 ttagtcaaga tcaggcgttt caaaatgcat tgctgctgac agagcaagta ctgacagaac
     3601 aaaatgtgaa ctttatattt atcgataaaa acgaacgtgt catttatccg actaatacag
     3661 ctttactgaa ttttacctta acagctgatc aatggaaaaa tctgaggaat ggacgtcaag
     3721 aaaaattcac atcgaataaa aatattttag gacaagcaca ggcgacctct tacgcacttg
     3781 tgccatttaa tttgaatcat gaattctatg gagcacttgt agttagtcaa cctgcgcgaa
     3841 atatcgataa cagcgttcgt ccaattacac tgaacctgtt caaaggattt attttttcca
     3901 gtatcgttgc agttattgcc agctatgctt ttgcagcatt ccaagttaag cgtatcaatc
     3961 ggttaaggaa tgcgacgaaa gaagtgacga atggtaattt tgatgtacag ctccccgtac
     4021 atgacaaaga tgaatttgat gagttagcag atgatttcaa taaaatgacg aattcattga
     4081 aagaatctca agcagaaatt gaagaacaag aaaatcgtcg aagacaattt atggcagatg
     4141 cttctcacga gatgagaaca cctcttacga caatcaacgg acttctggaa gggttagaat
     4201 acaatgcaat ccctgaaaat cagcgagaaa acgcgattaa attgatgaaa aatgaaacag
     4261 agcgtctgat ccgtttggtg aatgaaaact tggattatga aaaaatccgt acgaatcaaa
     4321 tcagtatggt cattaaaaaa ttcaatggaa cagaaacatt gaaaaatatt gttgcacagt
     4381 tagaagctaa agcagaagca gcaggagata cattgacatt gaaagccgtt gatgatattg
     4441 atgtttacgc tgattatgac cgatttgtcc aaatcatggt caatattatt caaaacgcga
     4501 tacagtttac tgaaaacggc caaatcacga ttactttaga aaaaggctat cttgaaacga
     4561 tcatcacgat tgaagatacg ggaattggaa tgtctgaaca acaaatgaaa agtatctggg
     4621 atcgttatta taaagtagat ccttcaagaa aaaatacaaa atatggtgaa tcaggattag
     4681 gtttaccaat cgtgcagcaa cttgtacgct tgcataaagg aaaattagaa gtagaaagcg
     4741 aactaggaaa aggaacaaaa ttcgctgttc gtttgcctga cgtagaaatc aaaaaagagt
     4801 aaaaaacaag cggtataaaa gatcatgaat cgatcttcta taccgcttgt ttttactata
     4861 aaatgattga tttagcccgc gggctctaaa cgatcaatat aaggactttg agcaatagta
     4921 gcatcatgag taaagagtaa gataccttga ttttcttcat gagctaaata agaaagcaag
     4981 tggagtagct cttggatctc caatacacta agttcatcaa atgtatcttc aataacaata
     5041 ttgtttcgct tggaaagcaa ggcatgaagt aattgcagct ttactttttc aaaagacgtc
     5101 aactcagctg ccgcacaatt aagcaaggaa ggagagatat caagtttttc ttgataggcc
     5161 aaacgatctt gtttgatttc tttttccggt acatccaaca ggacatggtc tttaagattc
     5221 agataaggaa acatcggcca atttttttgg atgatcgcaa aattatccag ttttgcaaca
     5281 agaacctctt ttttcaagtg ctccaatatt ttatgctgtt tttcgagagg acacaaaata
     5341 acggtcaagt gttcagcatg aatcaaatat tcaggtttca ttacgtccag gcctcctact
     5401 ttgcaaatga tggataaaaa aagtgaaagg gaatagacta agaatgagac agcatccact
     5461 gagcccaata agagtttggg caaatccctt aagtatatct gtgaaaaaaa gatcattttg
     5521 gaaatcgatg gtaaaaaaag tctgttgatc catcggaatt gtaagtccgc ttaccgaagt
     5581 caagatatca cggatatctg gcagttcagt agtctttaag acaagaaaat tgatcctttg
     5641 aagaagcgct tcataaactg actggaagct tacgatccac aataaaacca agatacaaca
     5701 aagaaagaga ggaaagaaca gctgccagaa aataaaaagg aataatttgg ttttagaaag
     5761 accagtcagg tgccaattga tcaattcctg cttgttttgc ctgactgaat agtaaaagaa
     5821 aaagataaaa aggaaggcaa atagaaaaaa taaactgaat atgcctttta tataaaatcg
     5881 gctagctaat tctaaagccg gaagaagctc tgtagatgca gaagcgccaa taaatgaaag
     5941 ccgatcttgg acttgattgg ctaaagccga ttgcaaatgg taaagagtca ggcatgctat
     6001 caataaaaaa gcagaaaagc ttgaaaataa acaatagagt attgtctgct ttttgtaata
     6061 aattaatcga gcagtagcat agcggattat tttcatactt ttcacctcta taaaaagtat
     6121 agaaaatgaa tatgaataaa gtatgaaatt cctttagtga aatcacgaat taaaaccaga
     6181 aaatattttg taaaaagcaa gaggttgtga agaagctgcc ctattctatt accgtagatg
     6241 caagcttttg aacactgttt attcgaaaag aggagcagcg acaataattt tgtcgctgct
     6301 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
     6361 cgtttgcgtt ttataaagat caacagttga ttgtccgttg tgttcactaa agacactagt
     6421 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
     6481 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
     6541 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
     6601 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
     6661 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
     6721 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
     6781 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
     6841 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
     6901 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
     6961 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
     7021 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
     7081 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
     7141 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
     7201 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     7261 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     7321 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     7381 actaatgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     7441 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     7501 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     7561 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcatgctgga gaccattctc
     7621 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     7681 attttttcct ctagcgagcc cgaacaaatc gtcatttggt gctgcataat gttcttttac
     7741 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     7801 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     7861 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     7921 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     7981 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     8041 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     8101 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     8161 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     8221 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     8281 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     8341 taaataggca aaaatcgttt tcaaccaaag taaaacgact agtaaagaaa aaaatccgag
     8401 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     8461 tcttaatcac agaagtgtcc tgaaaccatg ttacacagct cacttattct catttatttt
     8521 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     8581 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     8641 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     8701 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     8761 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     8821 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     8881 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     8941 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     9001 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     9061 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcatag gaggctatga
     9121 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     9181 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     9241 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     9301 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     9361 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     9421 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     9481 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     9541 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     9601 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     9661 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     9721 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     9781 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     9841 ccattagaag gagcgatgca gaatggttcg ggtaaaggga ttgaatatag gaagcggtag
     9901 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     9961 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
    10021 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
    10081 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
    10141 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
    10201 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
    10261 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
    10321 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
    10381 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
    10441 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggttgg
    10501 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
    10561 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
    10621 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
    10681 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
    10741 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcgt atatttaaaa
    10801 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
    10861 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
    10921 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
    10981 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
    11041 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
    11101 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
    11161 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
    11221 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
    11281 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
    11341 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
    11401 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
    11461 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
    11521 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
    11581 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
    11641 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
    11701 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
    11761 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
    11821 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
    11881 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
    11941 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
    12001 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
    12061 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
    12121 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
    12181 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
    12241 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
    12301 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
    12361 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
    12421 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
    12481 atctgaagaa agaaaacaga aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg
    12541 agggaagata agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa
    12601 ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg
    12661 aattcatgcc cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata
    12721 tcttagaact tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg
    12781 atgaaggaaa ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc
    12841 cttataaagg tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg
    12901 cttttgaaca gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg
    12961 gaagtgactt cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt
    13021 tcatgctgga attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg
    13081 gtgtaggagc tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg
    13141 atactggtgt gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag
    13201 ctactggtta tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat
    13261 ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa
    13321 aagtgcaaga gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg
    13381 atccagaagg aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt
    13441 tgacggaata cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga
    13501 cattgaaaga aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa
    13561 gcttggccga aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc
    13621 catgtacagt aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa
    13681 aagctgctaa tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgtccagc
    13741 gattgacttg ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt
    13801 accatacgtg tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag
    13861 caaatgttgc tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa
    13921 aaatgaaact ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa
    13981 aaaaaacgta aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca
    14041 ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc
    14101 aaagccaagt aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact
    14161 tccgcggctg gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag
    14221 aagcagctaa aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt
    14281 cttatttagg cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag
    14341 atagcaacac aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat
    14401 atattgctga cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa
    14461 aatctggaac aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta
    14521 agaaatatgg acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag
    14581 gtgcagttaa agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca
    14641 tcggcggacg tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg
    14701 cagatatcga tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca
    14761 aactagaaga aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag
    14821 gcaaagtaac tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt
    14881 ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg
    14941 caaacttctc aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata
    15001 tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag
    15061 cagaagattt agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa
    15121 aagcatttga aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca
    15181 aaattccaga aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg
    15241 taggtatttc aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt
    15301 acaagaaaaa catgttcgca cttctaggca aaccaggctt tgaagatctt gctaaagaat
    15361 tgaacgaacg cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac
    15421 atgttcgctt taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt
    15481 attcagaata aggggctgtg acaagacgtt tgtc
//
LOCUS       DACEKG010000067        15352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEKG010000067 DACEKG010000000
VERSION     DACEKG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.070"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..563)
                     /locus_tag="IW664_002071"
     CDS             complement(<1..563)
                     /locus_tag="IW664_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0849421.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IW664_002072"
     CDS             complement(750..1400)
                     /locus_tag="IW664_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849422.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW664_002073"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW664_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0849423.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IW664_002074"
     CDS             complement(2912..3910)
                     /locus_tag="IW664_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0849424.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW664_002075"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW664_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0849425.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQSSVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IW664_002076"
     CDS             complement(4926..5657)
                     /locus_tag="IW664_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0849426.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IW664_002077"
     CDS             complement(5661..6926)
                     /locus_tag="IW664_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0849427.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW664_002078"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW664_002078"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0849428.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW664_002079"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW664_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0849429.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IW664_002080"
     CDS             12268..13770
                     /locus_tag="IW664_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0849430.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IW664_002081"
     CDS             13772..14509
                     /locus_tag="IW664_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849431.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="IW664_002082"
     CDS             complement(14554..14727)
                     /locus_tag="IW664_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0849432.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..>15352
                     /locus_tag="IW664_002083"
     CDS             14703..>15352
                     /locus_tag="IW664_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0849433.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAF"
BASE COUNT         4519 a         3287 c         2591 g         4955 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagattgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt tt
//
LOCUS       DACEKG010000068        15140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEKG010000068 DACEKG010000000
VERSION     DACEKG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.071"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(565..1131)
                     /locus_tag="IW664_002084"
     CDS             complement(565..1131)
                     /locus_tag="IW664_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0849434.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1131..2087)
                     /locus_tag="IW664_002085"
     CDS             complement(1131..2087)
                     /locus_tag="IW664_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0849435.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2087..2743)
                     /locus_tag="IW664_002086"
     CDS             complement(2087..2743)
                     /locus_tag="IW664_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0849436.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3113..5131
                     /locus_tag="IW664_002087"
     CDS             3113..5131
                     /locus_tag="IW664_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0849437.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5558..7972
                     /locus_tag="IW664_002088"
     CDS             5558..7972
                     /locus_tag="IW664_002088"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0849438.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8320..9873
                     /locus_tag="IW664_002089"
     CDS             8320..9873
                     /locus_tag="IW664_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0849439.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10254..12038)
                     /locus_tag="IW664_002090"
     CDS             complement(10254..12038)
                     /locus_tag="IW664_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849440.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12053..13774)
                     /locus_tag="IW664_002091"
     CDS             complement(12053..13774)
                     /locus_tag="IW664_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849441.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13874..14635
                     /locus_tag="IW664_002092"
     CDS             13874..14635
                     /locus_tag="IW664_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0849442.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4935 a         2770 c         2984 g         4451 t
ORIGIN      
        1 tttattttcg gaaaaagcta atatagctaa gattgaaaca tccatctgga atttatcttt
       61 tttactgctt tgatcataaa agtctgcata ttcatcactg atattttttc taataaattc
      121 ttccatcata aaaatcacct caaaacgatt atacgaactt acgttcttgt tgtaaagcga
      181 tatttgaggg gcaaaaaagg ggcataattt gactgatatt aagttagcta ttgatatatg
      241 aatgacatac aaaaatataa aatcttttaa aaatgcttat atatcaatgt ttttgccatt
      301 cttttatata tctgttatat gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaataa
      361 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt
      421 ctcatgtttt tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa
      481 taaagaggtt atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata
      541 tcatttatgt tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg
      601 ctggttcttt tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt
      661 ggaacaaaaa gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac
      721 gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg
      781 tgtagtctct ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa
      841 aatccctgct ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg
      901 tagaaacaat gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc
      961 gattgcttgt tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc
     1021 tagaaaaacc gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg
     1081 caagatttct tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc
     1141 acctttttcc gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca
     1201 attgcattca agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac
     1261 ggcgcatccc ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg
     1321 caaatgacat tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc
     1381 cgcaacattc tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct
     1441 gaatctaaaa tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat
     1501 ccgttgatca aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct
     1561 aaataagttt gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt
     1621 tgcaatccaa gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact
     1681 acttcatctg gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa
     1741 ttgactactt gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa
     1801 ttttggaaat aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt
     1861 tctttctgga attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca
     1921 gatacactgc aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca
     1981 aatcctccat cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa
     2041 ttccaagaat gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct
     2101 ttctgtaaga gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca
     2161 gccaagaaag actgagtaag tagccgccga tgatatcagt aggaaaatga acgcccagat
     2221 aaatgcggct tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc
     2281 gtaaggttct tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca
     2341 tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca
     2401 atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta
     2461 caataaatcc agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata
     2521 aagccgcaca agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga
     2581 gttggtagct gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga
     2641 aaccagaaag ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa
     2701 gaaagaagct accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa
     2761 gcaatgactt attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa
     2821 tgtatgattc ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag
     2881 acattttttg gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc
     2941 ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg
     3001 gtaaattaca atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt
     3061 aagagaagtt ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa
     3121 cattgcaaga aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg
     3181 aggggcagca atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca
     3241 gccggttcct gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc
     3301 ggcatttatc acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata
     3361 tgcatcagtg atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc
     3421 tcaagcacct tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta
     3481 catggatacg cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca
     3541 atatccttct tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt
     3601 tggcaacggt tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc
     3661 gacagcttgt ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa
     3721 tatcatcgca acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg
     3781 tggagcgact acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa
     3841 ttctggtaat acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga
     3901 ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa
     3961 caacatccaa aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga
     4021 aaatggctct tcgacgaata attccggtaa caataccaac tcttcaggaa atgctggtac
     4081 atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg
     4141 gaagatctca aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa
     4201 gaacaatttc gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc
     4261 tggagcaaat actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag
     4321 ttcttctaat acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag
     4381 cgtctcaaat aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa
     4441 taactttatc tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac
     4501 gtcaggtaat acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag
     4561 cagttcgtct caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa
     4621 taaaaacggc atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat
     4681 ttatccagga cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc
     4741 aactacttct gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc
     4801 cactggtaat acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa
     4861 acatcatatc acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta
     4921 tccaggacag caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt
     4981 tactggttcg aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag
     5041 tcatggaatt acaatggctc aactaatcga atggaacaat attaagaaca actttattta
     5101 tccaggtcaa acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa
     5161 ttccacaaaa atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag
     5221 tagtaaaaag actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt
     5281 tgaactcgcc tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc
     5341 gttacaacgt tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt
     5401 cctataaaaa gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa
     5461 acaaagcaaa aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat
     5521 agaaacaagt gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat
     5581 tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc
     5641 tgaaaagcca aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca
     5701 tgtagggcat cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca
     5761 aggatacaac gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata
     5821 tgcgctagat acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg
     5881 acgccaaatc aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga
     5941 tccggagtat tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc
     6001 ttatgaagca gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga
     6061 agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg
     6121 ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt
     6181 tgattggcca gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc
     6241 aaatattgaa tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc
     6301 agatactcta ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga
     6361 aattacgacc cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa
     6421 atcagatcta aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta
     6481 tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc
     6541 ttcatacggc acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt
     6601 cgctcaaact ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc
     6661 tgcttacaca gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca
     6721 agaagcaatc gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt
     6781 cagctatcgc ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc
     6841 aatcatccat tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg
     6901 gttgcctaag acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat
     6961 cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa
     7021 tacaatgcca caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa
     7081 caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat
     7141 tggtggagca gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt
     7201 atacgatatc ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat
     7261 gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga
     7321 tgatgtggtc gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc
     7381 attagatgct tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga
     7441 tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt
     7501 gaatgatggc aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta
     7561 tgaaaatctt catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta
     7621 taaagtggac gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat
     7681 tgctcctcac atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta
     7741 cgcaccatgg ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt
     7801 ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga
     7861 gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt
     7921 cagaaaagtt attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct
     7981 ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc
     8041 tgtctcagat tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt
     8101 tcatgttctc aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa gagatctctg
     8161 taatcatcaa atttttgtct attaatctta gtattttttg atgtataaac tacctgaaat
     8221 tgagagtaca taccaaattc ataaattttt tattagtggg actagtgatt gtatggattt
     8281 tgacgcataa tgaatgaagt atgagaaaag gagagacaaa tggatcaaga actatttatt
     8341 atcgaagtat tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga
     8401 ataatgttca agcttttaag cttagtattt ttctctggtt tgcttttttt cggagctgta
     8461 tcgttcctat atcgccatat gagtgcagta gtccgttcag ctgaagggat gcaaaaatta
     8521 aataatttgt atcacttttc gtggggagtt ggcggaataa caattgttgt gcttattgtt
     8581 tttcttctac ttatttggct ccgttaccat caattgtcgc gtcatttcga tcaatggctt
     8641 agtcaaagag cacaacagtt tttacataat aaaccaactt ctcaagatga tgactatctt
     8701 tattttgaga agacttcgac gagtcaagct tttcggatac aaaaaagtga ttgccagtta
     8761 ttgctgagat cacttgatga tttacctatc tacttaggcg aaagcaaatc aaaattcgga
     8821 atgaacacat tccatatttt tttactagaa aacgaaccca gatgggaaaa aagccagtct
     8881 tcgcctggtg gatacctgac aactaagatg agtacattag tcactggttt tttcctcatt
     8941 cttttagctg gaacaatcta ttatgggaga tacactcagc caaaaatgtc ttctcatata
     9001 aatgctgctt ctcacatgac aatgaactca actaacgata cgcatataca aaagacaaat
     9061 gaagggttgt tagctgatcc taagcaaaag gtaaatttga caacacaaaa gataaatgat
     9121 ctcaaacttg atacagagac gcactctctc tatatgacaa cagatagtgg tgctaattgg
     9181 gtatttgtgc cgattgattc gcaatggttg cgttttggcg aatacaccct gacaacgggg
     9241 ctgattcctg aaggttattg gatggacaac acatatgata tttccgccga tttctcatgg
     9301 tttatctatt cttctgggga tgatgtacgt ctgctgactt caagagataa tggtaaaaca
     9361 tggcaaacgt ctattatcac ccaatcagtt cagccgatac gttatcgaaa agcggtattt
     9421 tatggcaacg atggtctatt tatctgtaca tcagccagtg ggatgtctgc agaaactttg
     9481 tacatttaca ctactatgga tggcggaatc acttggataa aaagtggaag tacaatgatc
     9541 aatcagccag tacaaaatgt cagcttcgtt acaacttctt taggttttgt ttcgacacgt
     9601 gaaagtttgt tttatacaaa caacggagga cgctcattca aagaatcagt tctgtcgatt
     9661 ccggaaaact atcagacagg ggggatagat ctttttcaat caccaaatga agttactcaa
     9721 ataagcgcga atacgttaga gacaaaattt tatttgttga aaaaaggacc aatcgatgca
     9781 gggaaaatgt ttgcctgcct cttccgttca actgataacg gggaaacatg gcaattcgtt
     9841 cagcaactat cgcaagtaga acaaggagat taaagaagag agattgtgag cctgacgttc
     9901 agcaccgagt cacaagaaac aaattcaaaa aacagcttct catatttgtt gaatagtgta
     9961 actcgtgatt cggagttgac aagagcaaca agaagcgatt ttgaagtata gcttctcgta
    10021 ttttacaaga tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa
    10081 aagggattgt gacaagtctt ttgtcactac ttaccgaacg aaactgaagg agctatccaa
    10141 aaagtatcat tagtattatt ctttgtgttt ctagtggcac cactagtaca tttaaaaaag
    10201 ctatcgatca tggactaatc atacatgatc gatagctttt tagataatgt ttgttacgct
    10261 atctgaaatt gactattata aagactggca taaagcgttg gtgattggag tagttcagaa
    10321 tgtgttcctt tttcgacgat atcgccattc ttcattacaa ggatcatgtc tgcattttca
    10381 atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac agcttccatt
    10441 gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag aagttgcttc atctaaaata
    10501 acgacaggag gattcgctag aatgactcga gcaattgtca ataattgttg ctgtccttga
    10561 gataacgtgc cgttttcgct agaaatgatc gtatcatatc cgtctggcag tgttcgaata
    10621 aagtgatcac attgtgcgat ttcagccgct tcgataattt gttctcttgt ggcatccatt
    10681 ttcccgtagg cgatattatc agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc
    10741 atcccaaaag aacgacgtag ttctccacgt gaaagcttgg taatatcaag accgtcgaat
    10801 tcgatctttc ctttattcag ttcataaaaa cgcatcagca gattgactaa agtcgttttt
    10861 cctgcaccag ttggaccaac gatcgctacc gtttgttttg gctttactga aaaatcgact
    10921 tgattcatca atattttatc tgatgtgtaa ccaaactgta catttttgaa agctattttt
    10981 cctttcggtt gttctaaaag tcttgcatct ggaaggtctg gaatttcatc tgtttcatct
    11041 aatatctcaa atatcctatc gattgctgcc attgcggatt ggatcgagtt gatcacataa
    11101 gaagcggtag aaattggttc agaaatctga ttgatgtact gaagataagc ttgtaagaag
    11161 ccgatcgtca caccacctga tagaacaagc atagcaccga ataccgcact tatgatgaag
    11221 gcgagttgat taatgaaacg aatggccgga tagatagcaa agttcataaa ttgagcacgt
    11281 ttaaaagcgt tgtattgctt tttatttact tcgtgtatag cagcttctgc atgagcttgc
    11341 tgattgaatg ttttgaccac tagattacca gctaagattt cttccattcg attatttaaa
    11401 acacccagtt catactgatt ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt
    11461 gtaacaattg aactgccaac gatcagcaat aagacgagca aagtaagctt gctttcgata
    11521 taaaacagca tcagaccagc agcagctatt gttacgacag aagtaaaaaa ttgattgata
    11581 cctgtcaata acacttgaga aagttgattg agaccggtag ttgtccgact aatgatgtca
    11641 cctacttgat gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt tgtgatttct
    11701 tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc ctgaataaat
    11761 gaagtgatgc tgcttaagac tgaacagccg atcagcaata agacagggaa caacaatgct
    11821 tgtttgatat cactgaaaga aagctgtcca atacctgttt ttcttatttc tgataagaga
    11881 tcatcgatcc cgattcccat gatcaagggc atggctacga ctaataggtt cccgaataaa
    11941 ctgcatagaa acaatccgta aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt
    12001 atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg tatcccttct ttctatattc
    12061 cttgtgattt tgcaaactcc tgataagttt ggcaattaga cagtagttct tcatgagtac
    12121 cttgtccaac gatttttcct tcatctaaaa caatgatgtt atctgcttgt tggattgtac
    12181 tgaggcgttg agcaacaatc acgagagttt tctccgacat gtgtttatga agggctgctc
    12241 ttaatgctgc atctgtttta tagtctaaag cggagaaact atcatcgaat acatagatct
    12301 ctgctggttt gatcaacgct cgagcaatac atatccgctg tttctggccc cctgaataat
    12361 tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg aagggtgtcc actagttctt
    12421 ctaattgagc gatcttgatc gctttagaca actcttctgt tgaggcatat tcatttccca
    12481 tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc cttctgaggg acataactga
    12541 tgtgggagcg gatctgttct tgagtcattt gagtaagagg aataccacca agggttactt
    12601 ttcctatcgt agggtcacta agtctcaaga gaagtttcgc caaactactt tttccagatc
    12661 cggtcccgcc aacgatcgct gttgtttttc ctttaggtat tgtgaaatca atatcttcta
    12721 gaactggatc agagatttca gtatatttaa atgtgacatg ttcaaaagca gctgcatttg
    12781 tctgatcaaa ggttggttca gctactgttt tgatcgtctc ttcctcaatt tcgatctctg
    12841 cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga tttagggata atgaccaata
    12901 ccatagctgc gatcatcaag taagcaagtg tcagcataga gtattcgact acagcagtca
    12961 cgacaccgat ttgtaatgct ccttctgcta caagatttcc gccaaaccac aaaatagaag
    13021 cataaacgat tcccatgagc aaaaaagcaa ttggagtgac ccaagcaaat attcggttca
    13081 ctttgatcat ttgattagca tagtcagcaa agctcttatt cgttcgcttt tcttcatatg
    13141 attgattatc aaaggcacgg atcatattaa tcccagtaaa gaattctctc aatgtgatgg
    13201 tgatccgatc catttttggc tggatactca aagataaagg tgcccctcgt ttcatcagtc
    13261 gataaacaat ccatacataa aaaacaacag agatcaatgt aacccatgtt agtaatagag
    13321 aataacgaaa catcatgaga atggtgaaaa cgcaaataac cggtgctggt aagatcattt
    13381 ggcaaaataa aacaatcatt tgctggacat tatcgacatc attcgtcatt cttgtcaaaa
    13441 gagatccaga actaaaacga ctgatatcct tcattgacag ctgttggaat ttacggaaat
    13501 atttctcccg cgtttggaat ccaaacctag cggaagccaa tgctgaaaag taactagagg
    13561 caactgcagc gattgttcct agaaacgcta caagcagcat ttcaactcca atttttttta
    13621 taactgaggc ttcgccactt gctatacctg tgtcgatcag ctttgctact aattttggta
    13681 cacctaatgt accggcgatt tgtaaacaga tcatcacaaa ggtaaggaga gcgagtcctt
    13741 tgtttttctg taagaatgtt tttaagatag acatattatt cctccttttt tcctgatact
    13801 tttcgattct ataccttcaa gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa
    13861 gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa gtatcggaac tatttgattt
    13921 gccaaaatca acgatccgct attgggatga acaaggatta atccgttcaa gtagacaaga
    13981 agagaatgat tatcgattgt ttgatatcga tgatattttt atgctttatg atatcgtttt
    14041 ttatagaaaa ttagatatac cgatcaaaca gatgaagaat ttatatggaa aaacattgac
    14101 ggagttatat gagacacttg atgaaacaga aagaagaatc cataaagaac tggtggtgat
    14161 gaaacaaaaa caaaaagaga taagggagca aaaaaaacag ttgaaattga tgatcgatac
    14221 aaacgaggaa gaatttcctg tagaagagat cccttttgac tgtatgatct caacggagtt
    14281 tgaagatatt gtggaaatca aaaaatttct acctaactat tcaagctttg ggatgatgag
    14341 tatgccagct tccagttcac agacgatgta tggatttttt atcgacccta gtgaagtaca
    14401 tttatttact caagacgtga tttgggagaa aaaagatacg gctgtttatc gtcgattctt
    14461 attgaaatcc gagatgaacc atgcggaacg taataatatt ttagaaatca gagaaagaat
    14521 gtatgaaaaa ggatggaaaa caggagaagt gatcggccag tatctattga cgaatacgga
    14581 tgaaaacaat atcagaactg aatattatca tgcatggata gaaatgaaaa aataggaaat
    14641 atgtgtttaa gataactcga aaatgatggt tgttttagtt ataaagaaaa attttctggt
    14701 tcgattacgg gtttaatgtg ggggattatt agtcgtggat agatagaatt ttagtttttt
    14761 agaggaaata tgtctttaaa aatggaatgg ttgcaattct tagcgatata tacgatgtaa
    14821 ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg tgttatgtat aattcatggt
    14881 gaactgagac taatgagaag aaaaattcat attgttatga gtgagattgt atcagatgag
    14941 aatgataaat taaataggtg cataaaagca ttacacttaa aatacattgt tgcttacatt
    15001 attacaatat gttaataagg caaaaataaa tagatggatt gaataaacat attttttaat
    15061 caaatagtcc ctactgtgac tttcctcagc tatcggtgat actaccgata gcattttttt
    15121 gcttataaaa taattataag
//
LOCUS       DACEKG010000069        15011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEKG010000069 DACEKG010000000
VERSION     DACEKG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.072"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(275..1156)
                     /gene="galU"
                     /locus_tag="IW664_002093"
     CDS             complement(275..1156)
                     /gene="galU"
                     /locus_tag="IW664_002093"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0849443.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1199..2218)
                     /locus_tag="IW664_002094"
     CDS             complement(1199..2218)
                     /locus_tag="IW664_002094"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0849444.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(2851..3855)
                     /locus_tag="IW664_002095"
     CDS             complement(2851..3855)
                     /locus_tag="IW664_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0849445.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(3868..4917)
                     /locus_tag="IW664_002096"
     CDS             complement(3868..4917)
                     /locus_tag="IW664_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0849446.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(4934..5755)
                     /locus_tag="IW664_002097"
     CDS             complement(4934..5755)
                     /locus_tag="IW664_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0849447.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(5742..6521)
                     /locus_tag="IW664_002098"
     CDS             complement(5742..6521)
                     /locus_tag="IW664_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0849448.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6538..7008)
                     /locus_tag="IW664_002099"
     CDS             complement(6538..7008)
                     /locus_tag="IW664_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0849449.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7020..7439)
                     /locus_tag="IW664_002100"
     CDS             complement(7020..7439)
                     /locus_tag="IW664_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0849450.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7520..10288)
                     /locus_tag="IW664_002101"
     CDS             complement(7520..10288)
                     /locus_tag="IW664_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0849451.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10488..10712
                     /locus_tag="IW664_002102"
     CDS             10488..10712
                     /locus_tag="IW664_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849452.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            11491..13206
                     /locus_tag="IW664_002103"
     CDS             11491..13206
                     /locus_tag="IW664_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849453.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13209..14972
                     /locus_tag="IW664_002104"
     CDS             13209..14972
                     /locus_tag="IW664_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849454.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4362 a         3096 c         2585 g         4968 t
ORIGIN      
        1 ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc atttctctta
       61 tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcat aatattttct
      121 gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac tgatctagag
      181 tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt aataacaaat
      241 agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt agctttttac
      301 tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga agtccatatt
      361 caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt cctttaaact
      421 ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt agctgtatct
      481 cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga gtcagtagat
      541 aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt tctacaaacc
      601 cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt ccgtatttag
      661 atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt tcttcataat
      721 ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc cccagcatga
      781 cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc cccagtcctc
      841 ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca actaaagcga
      901 gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt gcatcaaagt
      961 gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg attcccgaat
     1021 ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga agcatttctt
     1081 ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca ggaatgactg
     1141 cttttttgac cttcacttgc tgttcctcct aaacgacgat taactattga tcatctattt
     1201 aacgaagaga aaattcattc tccatttttc cgtcacgtaa catgatttct tttgctgctt
     1261 ttttgatatc ttctccatta tacaaaacat tatagattgt ttgagtaatt ggcatctcaa
     1321 cacctagctg ttgagctagc tctactgctg cttttgtcgt tgccacaccc tcaacgacca
     1381 ttcccatgtt ttctagaact tcttccagct tatgtccctg tcctaacaag ttaccggcac
     1441 gccaattgcg agaatgaaca cttgtacaag taacgatcaa gtcccccaca ccactcaaac
     1501 cgataaatgt cagaggattg gcacccatcg cgacaccaag acggctgatt tctgccaatc
     1561 ctcgagtcat gattgctgct tttgcatcat ctccgaatcc taatccatgg atagcgccgg
     1621 caccaattgc aatgatattt ttcagtgctg caccagtttc tacgccaatc acatcattat
     1681 ttgtataaat cctgaaataa tcattcatga acagtttttg tacataggct gctgcttttt
     1741 gatcaatact tgcagctgta attgttgtga tatcatgtac tgccacttcc tcagcatggc
     1801 taggaccaga taatacaaca attgcctgtc ttttttcttc tggaatttct tcagccaaaa
     1861 cttctgagat tcgtttatgc gtatcttgtt ctagtccttt acttgcatgt ataatgattg
     1921 gcttagtatt cattttacca ataagttcct gtgcgactga acgaatcgct tttgttggaa
     1981 caacaaataa aatagcatct gcgttatcca ctgcttctgc caattcttta tatccgataa
     2041 tcgattctgg gatcgtcaaa tcaggtaaaa aatgctgatt tgtatgatac ttattgattt
     2101 catcaatctg ttcaggtaca tttccccaaa tacggacatc atgcccgttt tctgccaatg
     2161 tttgtgctaa agcagtgccc caagatccag gaccaagaac tgcgattttt tgtttcatgg
     2221 atattcctcc aaataaataa ttgataattt ccattatagt atacatttct taaagttggg
     2281 actaatttcc attgttttta agtgtttgtt tacttctttt atcgttttta cttgaaataa
     2341 tcaatgatat gttacaatag tctacatttt atttttacac tcgttatgct ttgcttgttc
     2401 ctaagaggaa gcagttttga caatgttact accagcatta ctattaggag gttaggaaac
     2461 ttctcctagt gagtattgat ttaaaataca taagagacag attgaacatt tctaaatctg
     2521 tcaggtcctg caacggacag attgtctctg ttgacggttg acataggaac ttttgtttta
     2581 gtcaacattc tggatacaaa acgctatgta aactggcgca actcgttgta cttggacttt
     2641 tactacggat ccatgcaaca actaaatagt actattgcat aacaaaaaat ggttggcacc
     2701 cacatgtccc attaaacaga ttataagttg aactgtagat aatgcaaaaa atgtgtttta
     2761 taacgtaatt gatgccaata ttttaaaaaa ataaagggag cagttatcgc tgctctcttt
     2821 attttttata ttctttttat ccagtctatc ctaataatca aactgacgat agtagcggcg
     2881 tatctctaat ggatggcagt tcaactgctc cacatgagca tcgatgcgtt gtgtcactac
     2941 atgcatcaat aagaaagaca aaacacgtcc gcgatatttt tcactgatac cttctactgg
     3001 atagtctttt gtgtctatca atgtataatt gctgcaaatt tttggcaaaa gtttagccac
     3061 acgttcagat aacactctct gttcatcttc gcctaaaaat aatgtgactg gggttgtttc
     3121 ttctacgatc tctaacgtcc cgtgaagaaa ctcggcagca tggatagatt tcgatggaag
     3181 ccagctttgt tcttcccagt aacacatggc atatgaataa acagctcccc attgattccc
     3241 tgctccaata aaataatgca tactatcacg tcgatgtttt tctgcaaatg ataagccaaa
     3301 tgcatctgct tttttctctg cttccactaa ccctgttggt aaataacgtt cgagctgttc
     3361 atagtattct gaataagctt cgaattctcc attcaaatac atcaatcgat ctgctgccat
     3421 aaaaaatttg atttgttctg ttccaggagc tggataagtg accacaaaat cgcatagctg
     3481 agacaactta ctgttcgctt tatcgataaa tccaatcagt gtcgctccta ctttcttgat
     3541 ttgttctact gctttcacta cttcttgagt tgtaccggta acagaggaca aaataacgat
     3601 agaatctttt gttaatcttt tgtttccagt agtgtaatat tctgcagcat gctgtacaaa
     3661 gacaggcaag ttgcttttcc cattcatata agtgacagct tgcatcgagg aagcatacgt
     3721 tccaccgatt cctagataat agacagcgtc aaatttttcc gcataaagtt tatcgatgat
     3781 ttcttctact tgcggacgta aagctagtgc gccttttatt gctttgattt gttcttcttc
     3841 attgaatttc agcatgattt ccacctctta taatttactt ttcaagactg tatcaaagtc
     3901 ttcaaaaatc tttttgttca agtcaatacc tttagctact gcggttttat aagcgaatag
     3961 ttgaaacgga acgacaagcg ctaatacaga cagatattca tcacattcta ttgctaatcc
     4021 aagtgttttc tcgtttcttg ctttttttgt ggtaatggtg agagtctctc caacgtaccg
     4081 actcatatat ctttgcaact tttgcgaacg ttccttattt acactttccc cttcaataaa
     4141 gaatagcaaa tgcgaagcat ctgcctccag gtatgggccg tgcatataag cttctaactc
     4201 aaatccttgt gacggaacgc ggattgtttc agtcaatttc gtctctgctt ctttagctgt
     4261 tccccagttt ggaccatatc caatggtgac gaaacgctgt gctaaacgaa ataaactttg
     4321 atatccatca aaaaattctt ccgttttttg aataatcgct ggtaaatggt tgattatttt
     4381 gcgcaactgt tgcatgtagt cttgttctat ttttttgctg attttatttt tacttattcc
     4441 aattcctatc cctaagagta gtaactgtaa aacagtcgca gaatagcctt ttgtaacgaa
     4501 gcctactgat tctaccccgg cacctaaatc aataatttgt gcagcttcta aagcaagtgg
     4561 actttgcacg tcatttgtga tagcaacaac aggtaaccct tgtttttgta tcatttttac
     4621 tgcttcaaca gtagaagcac ttttcccgct ttgtgacaca gcaatcacag tatcgattga
     4681 aggagaaagt tttccataat tgaaaaaata aaaaggttct tgaatatcga cagtaagatc
     4741 accataagat tccaatgctg gcttcgctgc taaacaagcg ttataagatg aacccgttgc
     4801 taagatcagt aaattcactg tttttttcat atgttctctt gtcgaaaaat cattcttttt
     4861 caagatcgta cttagtactt ccttttcttc atagatataa tcttgcatcg taaacatttt
     4921 ctctgctcct tttttaaaaa atccctatcc acgaaccaaa aattccaaag aaagctagtc
     4981 cgatcaagat cgttaatggt ttcacttctt tcttcaatag ataaaacact atgccaaatg
     5041 ctccaagaca taagatattt ggaacgatat catcaaaaat actttgaaca gtcacagcat
     5101 tttctcctga accgattttt aaaggaatat tgatggtgat catcgtggca gtcattgctc
     5161 caacgaccat cagtccaata atagacgcgc ctaatgtcaa actttccatc ataccgtttg
     5221 cctggatctt tttcaaaaaa cccgttccta agcgatatcc ccaacctgtt gctagatagc
     5281 gaaacagaat gtgcggaata ttgaaaatca aaatgaacaa gatagggcct aaaatatttc
     5341 cttgcatggc taaagaggta cccacacctg tggcaatcaa tcgcaaagtt ccccaaaaga
     5401 aagaatctcc taaccctgca agaggtccca tcaaagatgt tttgatacta tcgatcgcag
     5461 tcacatcaaa ttcaggatca tccttatttt cttcctccat tgcagccgtg attcctaaaa
     5521 tcagtgtaac gatatgtggg gtcgtgttaa aaaactctag atgccgtttc aaagctgctg
     5581 ccatttcttc tttcgactga tataactttt tcaaaatcgg aatcattgca taaacataag
     5641 ccatattcat ctgacgttca taattccaag aaaactccat ttgaaaagaa cgccaaaata
     5701 cgtgatttag ctcttttttc gttattttgc tgtttttgat ctcagaattc ttcatcttct
     5761 tctacctccc ctgcagttgt ttgaagtggt agttgacgga cattcataat attgactacc
     5821 acaactgcta aaatcgcacc aaaaatagct actccggtca caggaatctc taagtaagaa
     5881 gctatagcaa aacctaagaa aaaatatggt gcaacttgtt tgttgatcag caaacgagca
     5941 agcatcgcaa aaccaagagc tggaatcaac cctgtagcta ctgacaaccc cttttgaacg
     6001 aagtcaggaa tcatatttaa tagatttccg atcgtattgc ttcccaccat ataagaaacc
     6061 attaccacaa caccaagcat caaggaaaga ccgataccgg caagcaagtg catatgctcg
     6121 actcctttat aatttccttc ttctgcataa ttgtctgctt tatggttcat aattggaatc
     6181 aaaagcccta agtaaatatt tttcaatatc aatgtcaacg tagcaatcgg caaacctagt
     6241 aaaagcgcgg tttctgtacc ggctccagct gtaatagcaa aagccgtccc taaaatccca
     6301 ccggtcacaa catcaggtgg aatcgaagca cctactgaga acgatccgat aaatgctaac
     6361 tctaaagttg cgcccataat gatcccagtt tttacatcac ctaacacgat tcctgtaaac
     6421 agccccgtca caatcggacg agaaagcaac gatgtcccta gtgcatattc tgattgtgcg
     6481 acaaatgcaa caattcctaa taagactgct tgtacaatca ttgaggttct tcctttctta
     6541 caaaacttct cttacatcca cttttttatc tgctggtact tgacggattt ctacttctat
     6601 ttgtttgtca attaatcgtt tcaagcgttc ctcctcttct ggtaaaatat ttaccgcttt
     6661 tccgatgctg cgagtaccag gttttgcctt gatccctcct aagttgatgg ttttgatttc
     6721 tggatacgct tctgccagct tatatgcatc ctcgatcgat tccactacga tgaataactt
     6781 atatttatct gtcaccccgc ttttcaacgc ggcaatcgag tcatcaattg atttgatcac
     6841 tagtttgact ccttgtggct tggctaattt gatcgttgtc tttcgtatct cattcgtagg
     6901 tacatcatca ttagcaatca aaatacagtc tgcgcctatt gtttgtgtcc atgaaaaagc
     6961 tacttgtccg tgtaataacc gatggtcgac tcttgtcaat acaatcattt ttcttcctcc
     7021 taaaattcgt cttcttctat tttctgattg agccgctcgt tacaaaactg gatcattttt
     7081 tttgaatcag ttagcacctg ctggatcaaa acagttgtct ctacttcagc gtccaaccga
     7141 gatactagct caatcaatag tggcaaattc aatccggcaa cgagatagac atgatcccga
     7201 ttgatatatt gtaagaattc gttgttgaca cttccgccaa aaagatccgt cagaacaatg
     7261 atttctttat ctttgtacct cataaaaagc tgccttactg tttccgctaa atcaaaattc
     7321 tcatcagtat agcaatcgaa tacagtaata gaatgatttt tcccatagat cagctctaat
     7381 gaatctaata tccctgaagc aaattttcca tgggaagcta agataatttc tctatccact
     7441 ttttcatctc ctcttttcac ttacacatac taaaaaagca agttgcatgc caaaattagt
     7501 ggcaacaact tgcttatgtt caaaattctt cttctatcga gagaaactct gtatcttcac
     7561 tcaaaatatc atagatatag gcaatctcag aattggggat catgacacta taatgctctt
     7621 caatgacact aaacgcagat ttaatagcag ataacccttc tttttgacac tgcttccatt
     7681 tctctaatcc ttgataggtt tcgattggca tttgtcggat caatcgttca atgagacagc
     7741 tcacatggac atacaacgct aattttttgg gattgctgat gcttcgattc aactgcacct
     7801 ctaattctct cataaaatga tcgatttctc ccatcacctt ctctgtatct agaatagtca
     7861 cggaatccag tactttttcc aatgaaaaat tacgaatgat gcgctgattg aatatttcct
     7921 tctctgaagc attcatagta gtgtccaacc attcagctag tacagacatc ttttcccccg
     7981 agatgaggtc ttctaatgaa agatagtcga tattggtgat atcaggatct gtggtgccaa
     8041 tcattcctac gacttcgtat atggaaaata atgggtcact gtttttctta tcttttaatt
     8101 gctgatattc atatgggatg attttcaact gacaagccgt aggtaagctt ttttctagca
     8161 gttcacttaa gtggaccgcc gtaccgattc cagtgaagca agtagtaatg agtgctttgg
     8221 gtttgttttc ctctggatag agcagtcgcc attctggctg gatctgtcga acagtctctt
     8281 ctgctatctc ttctaatgat acattttttt gcaggcattc ccctactgtg attgctaaag
     8341 gtgtcgtcac attattcaaa atcaaaagcg gtacggataa ctgtttttta aaaaactgat
     8401 ggatttcttt cagagatccc atgtctacta atatgatcaa tccatttgaa acatcgctgt
     8461 tttcgcaata atcaaggatt tctgaagcaa tctgctgtgg tgtgacatct agtggcatat
     8521 caaatgattc aaatagatcc tcttgtaata aacgatttac tacatttgcc atactgctag
     8581 cagttgcata accgtgagca gcaatcatcg cttttggcag tcgtttttct tttgaccatt
     8641 tcgctttttt tagatagatc gtcagtaaaa tccgatccat acttgtgtct tcaatatcta
     8701 atttgatctt acttaattcc agaattcgtt ggacatagtg ataactagat ggataagact
     8761 gagaaacttg ttgttctaat tctttgatta gttggataat atcatgttct tccggtaacc
     8821 atttgatagt gcttcgctga tagagatagt aactcaaggc atagacactg tttccatcga
     8881 atttgatttg ataggcagtt tccatttgtc gaaaggtctc tcggatagat tgtgtccaat
     8941 aaaccagcag ctcgtgtttt tcttgtctac tggtctcaaa cagcaaaaaa tcaaataatt
     9001 gaatcacttc ttgctttaat tttccttcgc aagaatatag cagacagtta tttttcttga
     9061 aatcaattag gatttgctca aaacttttaa cgattcgctg ctgttcgggc tgttgcttct
     9121 ccattaattg ctctaaggtc tggtcttgcg taatatagat tccttgctgt gctgtatttt
     9181 tagtaatagg ctgcgcaagt agttcttttt gcaaatgatg gatcgtgaca tggatttcag
     9241 gctgttcacg ctgttcagca tatgcttttg ctgctgaaac tttgacgata tttttcagct
     9301 ctcctatgtt tccggataat ttctgacttg ttaaaagtga caaagctggt ccactgactg
     9361 acaaagggct ccccattttt ctttgttctt cgagaaaaaa agagtaaatc agttctagcc
     9421 gttcattacg actacgctga ttaagtgcag gtagttcgat tcggataggt attcttcgaa
     9481 tgaaagttgt taagaaatga ctagtaatct cttcagtcgt agcaaaaaag agacgtgttt
     9541 tgatccggcg tgacaaatta ggttctccca tacgataaat cacaccttga tcaagatagg
     9601 tgaacagttt ttcctgacct tctggtgtta agcgatgtac ctcgtctaaa aacaagatcc
     9661 caccatctgc tgcttcaaaa gccccttttt tattttcttc tgcaccagtg tacgctccct
     9721 taacatgtcc aaacaaattg ctagtcaaaa gttcaggatt atctgcatat tgtgcacaat
     9781 taaaagtgat gaatggtgca gaatcttcta gcagatcatg aagcagacaa tactgataga
     9841 ccaaattaac taaataactt ttacctgtgc cactttcgcc tgtaatcaaa agtggtaatc
     9901 cgccatctgg atagtaaaga gctgttttga tttgttcgat actttctttc aaactggcgt
     9961 cgtatccgat cataaaagag aataagtctt ttggttgcgc gaaaaaaggc tgttcctctt
    10021 tgagatcatc gatagaaaaa taaaaattcg tgcgtaactt aaaaaattgt ttttcaaaag
    10081 cttctttgtg gaaataaatc actggtcgag aattgatttt gactagtagt tgctcatcat
    10141 ttaactggtt caaataatga ctgactgtgt ttctttttac cccaaaatga tcagctaacc
    10201 tttgtgctgt aaagacctcg ttcatatgtt ctaaatcgaa aaaggctgtc tggttctcta
    10261 agtaacataa aatttccttc ttcaacaact tattcacccc tcctccatca tagaaaacgg
    10321 attcattttg tcaataaaat ctttaataaa gaattgaaaa atatatctta ttatagacag
    10381 cagcctactt atcccttata atttttattc taaaaaacgt acagttcctt ccagttttta
    10441 tactggaatt tcaacatatg aaagaataag gaggctatga ctattcaatg aaatcggata
    10501 aatataaaaa caggaaacat acttcctgga aaacttttcg agaaatcatc ggatcttatc
    10561 tcgctgtagt tgtatttttg cctttgcttt atatggctat gacagaacaa actcgtttta
    10621 ttggcgtaga tacgcaatat atagccgaaa caggattttt attcatcttt atcttgattt
    10681 ttgcgttcta taaatccttc catacaaaat aagattcaca gatttcaact ttcctgtctg
    10741 tttttagcta ttacaaatcc ttatatgtgg tataacaaca atttattggc ataatgagga
    10801 aaaacgagtt atgatgcttt cagagagtaa agaggtactg ttgtcaccat cctttttatt
    10861 gattacgtac accactctta gttttttccc attgattacg aaaacaaggt agaaacattg
    10921 atgttcctac cttgttttct ttttttattt tcagatcaaa atctcaagta gcttttgact
    10981 tgatgtcggg aggttgtctt cgttccatca aacctgatag aatctgcgcc aaaccatagt
    11041 tttctccact attatttatt cctctaagat agtacccaca ataaacgaat aaatattttt
    11101 ctcattttca tactccttct ttgaaaatca attagatttc tctcaaccca cttttttaca
    11161 aaaattacgt agcaaaaaag attgcaagaa caaattaaaa aagaagtaaa tagagtttca
    11221 tctataaaca tttacttgat atatttttta tgcagaattc atttatagaa aaattaagta
    11281 aaatagacgt tcctatgaat gaagagactg gcatgttttc tcattttctt ttttttaaaa
    11341 aaaatagtct attcaatttc taaagcttct tcataataaa actttcattt atgaaaaggc
    11401 ttgttctctt ttcattctaa atttcctaag ctacaatgtg gtagtacttg ttttttcaat
    11461 tgtggacaaa taaataatag gaggaatcat atgattcagt tattacgtta tgcaaaagac
    11521 tatcgtaagc aaatcatttt gggcccgttc ttcaaatttc ttgaagctgt cttcgaactt
    11581 gtcctgcctt taatgatggc ttccttgatc gataatggac taaaaatgaa tgaccgagga
    11641 aaaatcattg aaatgggctt atggatggtc gctatgtctg tgattggtct gatttgtgct
    11701 atcatttgcc aatactatgc ttcagtagct tcacaaggat ttggtacaga gttacgtaat
    11761 cagctaatca aaaaaatcaa tactttctct cacaacgagt tgaatcattt tggaacagat
    11821 accttgatca cccgaatgac aaacgacatc aatcagcttc agctagcttt agcaatggtc
    11881 attcgtttat tgatcagagc accgttttta agtatcgggt cagtggtcat ggcttttgtg
    11941 atcgactggg aagtaggtct gtttttctta gcattattgc ctattttctc aatcatttta
    12001 ttttttatca tcaaaaaaac cgttcctctc taccaaaaag tgcaagaaaa actagatcag
    12061 ctcaatgaac acgttagtca gaatcttagc ggtgttcgtg tcatccgcgc atttgcgcag
    12121 acaaaaaaag aaacagagaa gttcgataaa agcacaaatg atctagcgaa caattattta
    12181 cgtgtttcca atatctctgc cctattatca cctgctacaa ccttgatcat gaatgtcggg
    12241 atcatctgtt tgctgacagt tggcgggatc aaagtaaata ttggtagctt gcaacaagga
    12301 caagttcttg ctttgatcaa ttatatgaat caaatgcttt tggcactgat cgttgtttca
    12361 aacctcgtcg tgatcttcac acgtgccgaa gcttcaggaa atcgggtgaa agaggtgcta
    12421 gacacagaaa attcgattct tgaagctgag gtcagtacta ctcccgattt ttcttctgaa
    12481 gcaattatcg cttttgacca tgttgacttc cgttatactc ctgattctgg tctttctctc
    12541 caaaatatta catttcagct gaagcgaaat acagtccttg gaatcattgg tccaaccgga
    12601 agcgggaaaa cgactttgac acaattgatt ccgcgtttct atgatgtcag ttccggtaca
    12661 gttataatgg acggaatgga tgttcgttct tggcagctgg atcatctccg tcagcaaatt
    12721 gctcttgttc cccaaacttc tgtactattt accgggacta ttcgtgaaaa ccttcaatgg
    12781 ggtaagaaag acgccacaga aaaagaatgt tgggaagctc tgaaaatcgc tcaggcagaa
    12841 gaatttgtcc gtcagcttcc aaatggttta gatacaaaag tcatggaaaa tgggaaaaat
    12901 ttctctggcg gacaaaaaca gcgactgacg attgcaagag ctttgattgc caagccagca
    12961 ttactgattt tagatgactc tttaagtgcg ttggattatc aaacagactt ggatttgcgt
    13021 aaggcgctac agacttatct tggcagtaca gtgattatta tctcccaacg tgtccgctct
    13081 attcaagaag cacatcatat cttagtgatg gatcaaggta agattgctgc tcagggtacc
    13141 catgaagaat tacttgctca atcagcagaa tatcaggaaa tcgtcgcatc acaggaggaa
    13201 gaatagttat gaaagtaaaa aaattatcct tgcagacgct ccgccgattc attccttacc
    13261 ttactgccta tgcaaaagac atttggctag ctgtgatact aggaatcatc aatgggattg
    13321 caacagttgt gatgacgtta caaattggtc aaagtatcga tcaaatgatt ggagaaggac
    13381 aggtccgctt caatcgactg ttccatttac ttattctatt cggtggtatc gttttaatca
    13441 acgtagtcag tcaatggctc atccaagtgt taagcaatcg gttagcttac ttatccgttg
    13501 caaaattaag aaaagatgca tttgctcatc tgaatcgatt gccacttcgt tattatgatc
    13561 aaaactccca cggaaatatc gtcagtcgat ttacaaatga tatggacaat atttccattg
    13621 caatttctgc ggccttcaat caattatttt ctggagtggc aatcatcatt ctttcgttta
    13681 tttttatgat gcgtctaagt ccacttctga cattggtcgt tctttgttct acaccaatta
    13741 ttttccttgt gagttggatc gttgctcgtg cttcacaaac aaatttcgat agacagcaaa
    13801 ggattattgg ggatatctcg ggctttgtga ccgaacgaat cggtaaccag aaaatcgtga
    13861 aagcttttca gcaagaagaa gctagtcaac agcaatttga actgctaaat gctgatctat
    13921 atgaaaaagg acaacgggca caattttctt cttccttgac aaacccgtct tctcgttttg
    13981 tagatcacct tgcttattta tcgattggtt tagttggcgg cctattggca ttgaagagtg
    14041 gttcgtcaat cactgttggt gtgatttcca gctttacgat ctactcaagt cagttttcta
    14101 aaccatttat cgaactttct ggaatcacga cacaactgca aaccgctttt tctggtctag
    14161 aacgagcatt tgatttgatc gatcagccag aagaaaagcc tgatttgcca gatgcctatg
    14221 ccttggaacc caaaaatatc aaaggaaaag tctcttttga tcatgtttct ttttcttatg
    14281 accccaagca gcgtttgatc gaagatttca attttactgc ttatccaggt caaaccattg
    14341 ctatagtagg aaaaacaggg gccggaaaat ccacacttgt taatttgctg atgcgttttt
    14401 atgacacaac aaaaggaaca attacagtcg atgaccatga catccgctct atccaacgtg
    14461 atacattacg taaaagtttt ggaatggtgt tacaagatac ttggctattt gacagtacat
    14521 tgcgagaaaa tcttcgtttt gggaatccgg aagcaagtga cgcagaaatc aatgatgccc
    14581 taaaagcctc ttatatgtat gaatttgtag aacgtttgcc ggaaaaatta gatactaaga
    14641 ttggcggtca agggttaaag atatcagatg gacaacgcca actgttgacg attgctcgaa
    14701 cgatgatcag taatccccct atgttgatct tagatgaagc taccagctca gtcgatacat
    14761 tgacagagaa aaaaatccaa caagccttct tagcaatgat ggaaggaaaa accagttttg
    14821 tgattgccca tcgcctgtcc acaatcaaaa atgcggacca gatattagtg atggatcagg
    14881 gagctattgt cgaaatgggc acccatgatg agctgcttca aaaagatggt tattaccgac
    14941 ggctctatca agcacaattc acagcacaat aaaaacaagc agtcttctgg gatgggaaga
    15001 ctgcttgttt t
//
LOCUS       DACEKG010000070        14995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEKG010000070 DACEKG010000000
VERSION     DACEKG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.073"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW664_002105"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IW664_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0849455.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IW664_002106"
     CDS             complement(322..891)
                     /locus_tag="IW664_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0849456.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IW664_002107"
     CDS             1077..1979
                     /locus_tag="IW664_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0849457.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW664_002108"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IW664_002108"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0849458.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW664_002109"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IW664_002109"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0849459.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW664_002110"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IW664_002110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0849460.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IW664_002111"
     CDS             8547..8732
                     /locus_tag="IW664_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0849461.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IW664_002112"
     CDS             complement(8794..9990)
                     /locus_tag="IW664_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0849462.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IW664_002113"
     CDS             complement(10160..11596)
                     /locus_tag="IW664_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0849463.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IW664_002114"
     CDS             11889..12698
                     /locus_tag="IW664_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0849464.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..14881)
                     /locus_tag="IW664_002115"
     CDS             complement(12884..14881)
                     /locus_tag="IW664_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0849465.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4378 a         3178 c         2660 g         4779 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcactacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DACEKG010000071        14911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEKG010000071 DACEKG010000000
VERSION     DACEKG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.074"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(60..1007)
                     /locus_tag="IW664_002116"
     CDS             complement(60..1007)
                     /locus_tag="IW664_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0849466.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1197..1466
                     /gene="rpsN"
                     /locus_tag="IW664_002117"
     CDS             1197..1466
                     /gene="rpsN"
                     /locus_tag="IW664_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0849467.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1642..2004)
                     /locus_tag="IW664_002118"
     CDS             complement(1642..2004)
                     /locus_tag="IW664_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0849468.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2015..3136)
                     /locus_tag="IW664_002119"
     CDS             complement(2015..3136)
                     /locus_tag="IW664_002119"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0849469.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3152..3502)
                     /locus_tag="IW664_002120"
     CDS             complement(3152..3502)
                     /locus_tag="IW664_002120"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0849470.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3632..5143)
                     /locus_tag="IW664_002121"
     CDS             complement(3632..5143)
                     /locus_tag="IW664_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0849471.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5465..6817)
                     /locus_tag="IW664_002122"
     CDS             complement(5465..6817)
                     /locus_tag="IW664_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0849472.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6970..8046)
                     /locus_tag="IW664_002123"
     CDS             complement(6970..8046)
                     /locus_tag="IW664_002123"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0849473.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8242..9987
                     /locus_tag="IW664_002124"
     CDS             8242..9987
                     /locus_tag="IW664_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0849474.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10236..10703)
                     /locus_tag="IW664_002125"
     CDS             complement(10236..10703)
                     /locus_tag="IW664_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0849475.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10687..12060)
                     /locus_tag="IW664_002126"
     CDS             complement(10687..12060)
                     /locus_tag="IW664_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849476.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12381..12869)
                     /locus_tag="IW664_002127"
     CDS             complement(12381..12869)
                     /locus_tag="IW664_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0849477.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12891..13493)
                     /locus_tag="IW664_002128"
     CDS             complement(12891..13493)
                     /locus_tag="IW664_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0849478.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(13562..14901)
                     /locus_tag="IW664_002129"
     CDS             complement(join(13562..14460,14460..14901))
                     /locus_tag="IW664_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0849479.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         4249 a         3178 c         2637 g         4847 t
ORIGIN      
        1 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
       61 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
      121 gcttggatga catgcttaag ggaagcagac agattcattg aaataaaaac atgaaaggct
      181 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
      241 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
      301 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
      361 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
      421 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
      481 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
      541 ccgaccgcac ccgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
      601 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
      661 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
      721 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
      781 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
      841 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
      901 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
      961 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
     1021 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
     1081 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
     1141 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
     1201 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
     1261 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgcaaa cttcctttag
     1321 atgctcgtcc agaacgattg aaaaatcgtg atttgatcga tggacgccct cgcggataca
     1381 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
     1441 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
     1501 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
     1561 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
     1621 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
     1681 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
     1741 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
     1801 tattcctgtt ccagtagctt tgatcccaat atgagtcgga agcttaggtt tagccatttt
     1861 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
     1921 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
     1981 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
     2041 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
     2101 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
     2161 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
     2221 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
     2281 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
     2341 ttccttcacc tgctgggagt tttttgactt gaattaattc agaaaccagc tcaagtgctg
     2401 gctgcagtgg ataaacttcc ggtagtgcgt gcccagaggg attcagtccg tacattgcta
     2461 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
     2521 tgacatgtat atatcgagga agttcgggaa gtttttttag cacttctata aagcgttctt
     2581 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
     2641 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
     2701 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
     2761 tctctagctt acttttctct aaaacagcag ctgctgcctg caaccattcc cgtgtacctg
     2821 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
     2881 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
     2941 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
     3001 gaccgtatcc gtttgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
     3061 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
     3121 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
     3181 ctgtttcatt tgtatgagta atactgaccc atactttgcc ttcatgaggt gaacgcgtca
     3241 caacaggctg accgttttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
     3301 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
     3361 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
     3421 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
     3481 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
     3541 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
     3601 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
     3661 cgcgtttttt atcgttgtga cgtttaccgc catctttttg gctgttatta tttttgctat
     3721 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
     3781 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
     3841 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
     3901 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtacttat
     3961 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
     4021 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
     4081 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
     4141 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
     4201 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
     4261 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
     4321 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
     4381 attcgtctac acggcgtttt gtacgtccga agacgattgt tagttcaggt gtttgaacat
     4441 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
     4501 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
     4561 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
     4621 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
     4681 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
     4741 tgatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
     4801 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
     4861 cgcgccctaa acgatataat tcttcttgtg tttggatagc taactcacgt gttggtgcga
     4921 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
     4981 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
     5041 ctagtggaat cgtttctgat tggataggtg ttgcttcttc aaaaccagcg cgttcgactg
     5101 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
     5161 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
     5221 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
     5281 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
     5341 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
     5401 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
     5461 atttttattt catttgctga agctgttcta ccacttcaaa taagcccatg ctgttgctgc
     5521 cttttagcat aatactatct gttgcttcaa gattttgttt tacagcatca atcaatttat
     5581 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
     5641 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
     5701 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
     5761 acacagcgat tctgcgtcct tttgttggta gatttttaaa agtatctaat accagtccca
     5821 tggctgttgg atttgcatta tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
     5881 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
     5941 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
     6001 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
     6061 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
     6121 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
     6181 cgtctggtac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
     6241 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
     6301 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaag acgatctttt
     6361 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
     6421 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
     6481 tggttttacc attgcttcct gtaattgcaa cgacttctgc acctactttt tccaagtaat
     6541 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatctt
     6601 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
     6661 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
     6721 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
     6781 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
     6841 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
     6901 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcttcttctt
     6961 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
     7021 taattgaatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
     7081 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
     7141 gtttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
     7201 agctaacttc gcgtactctt gcgctttttg ataaacttct tccggcactt cggctggaat
     7261 ctgcatttcg attttattat tgatatattt ggcttcataa tcatagaatg ctacgtcttt
     7321 tacgacttcg ccaggcaaag tcgtccgaac atcttcattt cctaatacag caacttcgat
     7381 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
     7441 taaagcattt tgcagctctt ctcggttttc tgcctttgta atgccgacac tagaacccat
     7501 attcgcaggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
     7561 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
     7621 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
     7681 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
     7741 cccgtttggc ccgtgtaaaa ctggaaaaac gatggctcct tcttctttga tttctcccgg
     7801 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
     7861 caagacatct ttgctagcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
     7921 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
     7981 aaatgcggaa agaatggaaa cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
     8041 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
     8101 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagttggttt ctaaaagaac
     8161 aaacgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
     8221 taaagaaaag gagggatgat gatggcgcaa acaaatccac gtagccgctt ggcagaaatc
     8281 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
     8341 caggtaaaaa aagcctttga agaactaggc cccaccttca ttaagatagg acagatgctt
     8401 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
     8461 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
     8521 tcattatcag atatattcga tgatttttcc aaatcgcctt ttgcctctgc ttctatgggg
     8581 caggctcatc gcgccaagct gaagaacggt gactcggtcg tcgttaagat ccaacaccct
     8641 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
     8701 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
     8761 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
     8821 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
     8881 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga accatttaat gaacgccgaa
     8941 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
     9001 aagcttttag ttgagaactt tatgaaacag gtatttgatg atggtttttt ccatgctgat
     9061 cctcatccag ggaatctgct attccatgta ctaaaaaaag aagaacagac acaagctagc
     9121 cgaaaaactg aaatagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
     9181 tctacagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
     9241 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
     9301 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
     9361 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
     9421 gatgaaatca acttgcaaga agtattcaca cacgtcgtga cgatttgtca tcaaaataat
     9481 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
     9541 atccgcgtcc ttgaccctga agtttccctc atggaagtcg cttcgccttt tgcccaacat
     9601 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
     9661 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
     9721 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
     9781 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
     9841 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
     9901 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
     9961 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    10021 aaataagaag tgattttgca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    10081 ttcgaagagg ctacttctga agcaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    10141 cagtaataag aacaatcaaa aaggaacatg gagcacattt atcctgtttc cctgttccct
    10201 ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt
    10261 aaaccttggt caattgcctt ttttaacgct tgacaaaaag acatatgatc tttctccgta
    10321 aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt
    10381 tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact
    10441 cggacgccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca
    10501 ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag
    10561 acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata
    10621 accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca
    10681 tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac
    10741 taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact
    10801 ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat
    10861 ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt
    10921 attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg catcagtttg
    10981 ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac
    11041 acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa
    11101 ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc
    11161 tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta
    11221 cattatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag
    11281 aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac
    11341 aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc
    11401 atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa
    11461 aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg
    11521 cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct
    11581 tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat
    11641 agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa
    11701 attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga
    11761 aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc
    11821 aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac
    11881 taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca
    11941 attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact
    12001 taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat
    12061 gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg
    12121 gaaataaacc aattttacag aatatattct tgtaaaaaaa atacagaaag aagcataagc
    12181 ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag
    12241 tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc
    12301 tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta
    12361 ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga
    12421 aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa
    12481 acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt
    12541 tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact
    12601 ttatttagca aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg
    12661 taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct
    12721 ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca
    12781 atgatcggca caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt
    12841 tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga
    12901 ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt
    12961 ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact
    13021 agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta
    13081 atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa
    13141 ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca
    13201 atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc
    13261 gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga
    13321 acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc
    13381 gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca
    13441 ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt
    13501 ccccacatct tttttcattt attaaaactg cctccttatg aactgaaccc caaaagttga
    13561 actatttaat ggactgtttc cgatattcta ctggagatag tccatttaac tttagtttta
    13621 tccttttgtt attataccac ctaatatact catttaattt actttgaaat atctcaattg
    13681 atcggaattt ttctcgataa aaaaactctg atttaagcac accaaaaaaa ttttctatta
    13741 cagaattatc taagcaattt ccttttctag acatgctttg aataatgtta ttctctttta
    13801 attttttttg atattgtggc atctgatact gccatccttg atctgaatgt agaatcagtg
    13861 aaccctcagt tccctttttt ttaattgctt gttgaagcat ttcttcaatc agtttatatg
    13921 ttggactagt tgatatacta taactaatta tttctccgtt aaataaatca agtataggag
    13981 atagatagat ttttcttcct tttatcttga attcagtgac gtctgtcacc cattttttat
    14041 tgggtgtatc tactgaaaaa tttcgtttta acacattctt ggcaattttt cctactgttc
    14101 ctttataaga tttatatcgc tttattcgga tttgacaggt aagtcccatt tgggacatta
    14161 attttctaac tgttttatga ttgattgtat atcctttcat ctttaacgct aaagtgactc
    14221 tacgataacc ataagagttt cttgattctt ttacaatcgc tgtaatttct tgctttatct
    14281 tgctatattt atctggctta tctaattttt ttacccagta ataataagtt gacttcgcta
    14341 attgtgcaat tgaaagtaat agattcaatt taaattcttt tttgagctga aggattgttt
    14401 taaccttgat ttcttcttgc tcaaatcttg ttcttgaatc aaggtttcta acttttttaa
    14461 atatgcgttc tctgctctta aacgaataac ttcttcttca agagactcat ctttaagttt
    14521 tttaggaatg tttagcttgg aattcatact aatttttctg cccctttttt ggctctcaag
    14581 tgaagaagca ccgccttctt catattgctc tatccattta cttagagttc tatttgaacc
    14641 gatattaaat tttttagcag tttcttggat agaaagacca tttgtttcca tatattctat
    14701 aacatcaagt ttaaattttg tagtgtagct tttgccacct ccaaccaagc cttcccaacc
    14761 atgatagtta taaatcctta cccaatgtct aaccagtgta cgatttattt gatatttatg
    14821 tgcaagatat ttgtagccgc cttcgttatt taaatagtct gaaactactt ttttcttaaa
    14881 aacaaatgta tatttccgca aaaaaagcac c
//
LOCUS       DACEKG010000072        14361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEKG010000072 DACEKG010000000
VERSION     DACEKG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.075"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            201..2672
                     /locus_tag="IW664_002130"
     CDS             201..2672
                     /locus_tag="IW664_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0849480.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2775..4511)
                     /locus_tag="IW664_002131"
     CDS             complement(2775..4511)
                     /locus_tag="IW664_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0849481.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4508..5212)
                     /locus_tag="IW664_002132"
     CDS             complement(4508..5212)
                     /locus_tag="IW664_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0849482.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5343..5846
                     /locus_tag="IW664_002133"
     CDS             5343..5846
                     /locus_tag="IW664_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0849483.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5806..6144
                     /locus_tag="IW664_002134"
     CDS             5806..6144
                     /locus_tag="IW664_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0849484.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6165..7583
                     /gene="ffh"
                     /locus_tag="IW664_002135"
     CDS             6165..7583
                     /gene="ffh"
                     /locus_tag="IW664_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0849485.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7695..8273
                     /locus_tag="IW664_002136"
     CDS             7695..8273
                     /locus_tag="IW664_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0849486.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8277..8798
                     /locus_tag="IW664_002137"
     CDS             8277..8798
                     /locus_tag="IW664_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0849487.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8877..9431)
                     /locus_tag="IW664_002138"
     CDS             complement(8877..9431)
                     /locus_tag="IW664_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0849488.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9684..9959
                     /gene="rpsP"
                     /locus_tag="IW664_002139"
     CDS             9684..9959
                     /gene="rpsP"
                     /locus_tag="IW664_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0849489.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9971..10222
                     /locus_tag="IW664_002140"
     CDS             9971..10222
                     /locus_tag="IW664_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0849490.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10347..11138
                     /locus_tag="IW664_002141"
     CDS             10347..11138
                     /locus_tag="IW664_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849491.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11308..11829
                     /gene="rimM"
                     /locus_tag="IW664_002142"
     CDS             11308..11829
                     /gene="rimM"
                     /locus_tag="IW664_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0849492.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11819..12580
                     /gene="trmD"
                     /locus_tag="IW664_002143"
     CDS             11819..12580
                     /gene="trmD"
                     /locus_tag="IW664_002143"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0849493.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12716..13063
                     /gene="rplS"
                     /locus_tag="IW664_002144"
     CDS             12716..13063
                     /gene="rplS"
                     /locus_tag="IW664_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0849494.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13321..13614
                     /locus_tag="IW664_002145"
     CDS             13321..13614
                     /locus_tag="IW664_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0849495.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4824 a         2581 c         2914 g         4042 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaaccctt tttttttgct atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag
      121 gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat ccagaaaacg
      181 aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa aaaaagaaag
      241 taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa gaaggattaa
      301 cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca gtcgatccca
      361 cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc aatttgatct
      421 ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg acttttttac
      481 cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc aaaaagatct
      541 tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgagac gggatagaac
      601 aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag actggatatc
      661 aaattccagc agatgcagag gtcgtggaag ggaaactgca agttaatgag gctttattga
      721 caggcgaagc agatgaaata accaaagaaa taggtgatcc attaatgtcc ggcagtttca
      781 ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg tatatttccc
      841 agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg attgcttctc
      901 ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc ctgctttttt
      961 ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca atggaagcag
     1021 cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca ttagcattaa
     1081 gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc attgagacat
     1141 tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa aataaaatgt
     1201 ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat cagtcagtca gatcaattgg
     1261 aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca atggaagctg
     1321 ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt cctttttctt
     1381 ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg
     1441 aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa
     1501 aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa ccagtattga
     1561 cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga aaagaagcaa
     1621 gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt tctggagata
     1681 atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct gatcagtacg
     1741 ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt
     1801 ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgataaag cgtaacaata
     1861 cagtagctat gacaggtgat ggcgtgaatg atattctagc catgaaagaa gcagactgca
     1921 gtatagcgat ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg
     1981 attctgactt ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata
     2041 ttgagcgatc agccagcttg tttttagtga agaatatttt ctcctttttg ctctcactgt
     2101 tttcagtgat ctttgctctg acctatcctt tagaaccatc acaaatcaca ttgattagtt
     2161 tgtttactat cggacttcca tctttcttgc ttgctctaga agaaaacaaa aagcgaatca
     2221 gagggaaatt catcatgaat gtgatggaaa aagctgtgcc aggtggacta acagatatga
     2281 tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa tctaaataaa acagatgttt
     2341 ctacagcatc taccatgctt ttgattgcag tagggttcct cgttctctat aaaatctgtt
     2401 caccgctgaa taaattcaga agtcagatca ttcttttctg tgctagcggt aacttttttt
     2461 cggtcatctt tttgcataag ctgttttcaa tcaccggtat ctcagctgta tcacttttgc
     2521 tgttgagcat tctatttttc tcggcagatt cgatttttag gcatttgacc acattggtag
     2581 aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa
     2641 aatttctatc gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa
     2701 agaacaacca ctcgtgtatc tttcgaagca ggatgagtca tccaacttta aaacgcacga
     2761 gcagttgctc ttttttacat tttcggaatg gtaacggtga aagtcgaacc tgttccaaga
     2821 tgactgtcta cagtaatcgt accattaagg agttgcacgt agtcttttac aatcgctaac
     2881 cctaatccgg tcccgcctga atgtcggctt cgcgccttgt ctacgcggta aaaacgctca
     2941 aagatccgtt cctgatcctc aatgtcgata cctataccaa agtcttggac agaaaacgat
     3001 agatcatctg ttgcctgata gcggataata atctcgctgt cgctttttga atattggaca
     3061 gcattttcaa tcagattttt gataattgga taaaggatat ctgttttagt agtaaatggt
     3121 tcgttctttc caatcaatcg aatcgtcaac cgtttctttt cgatgattgg ctgataattt
     3181 tgaacgattt gctggaaata aggttctgta taaattgtct ggatctcgta agaattctcg
     3241 ccatcttttg acagctggat gatttcccga atcagtttat ctaaccggat cgcatctttt
     3301 tgcatgatct caagaaaaga agtcagagtt tgcgggtcat cttttgcacc atccagtaaa
     3361 gtctcggtaa aaccaatcag tgaagtcaca ggagttttta attcgtgaga gacgttgcca
     3421 acgaaatcct tttgtagctt ttctaaacgt cgcactttcg ttaagtcata cgccacccct
     3481 agaatctgtc cggtaccttc tgaattagta aagtagcgca aactcaagtc taatgtctgc
     3541 cgaccttctg taagggtgat ctcctgatgg atcagcggat gctcaggagt cacttgatga
     3601 atcagctgaa tgagttttgg ctcttgaatg acttctgtgt accgctgttc aggttgatag
     3661 cttaccacgt ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg agaatcagca
     3721 tcatcaatca ggaatacgcc aatcatcagt tcgttcagta acgtatataa ttgctcttct
     3781 gtggtagtac acgcccgata catcttactc atttgttcgc tcaacgcatt aatcgtctga
     3841 tacaattctt cccattgatc gggtgtttgc atgattactt ccgcctgatt aggattttgc
     3901 accatttttt ttagtacggg cagcacggtt ttcaatggct ggtttttttg atagatcaga
     3961 taatagatca ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa gacccaacgg
     4021 cgaaaactct cagttctcgg taaaaaaacg cttgtcggtt ctgcgatacg taaaatggct
     4081 tctaactggc cacttttttt gactggtaat gcgacatata aaagatcctc attcaaagta
     4141 gtacttttgc gcaaagcgct acctaggttc ccaccattca atacggcttt gatttcggga
     4201 cgggagtttc gctgttcatt caatgtttca ttgtttgtgt caaataagat ctttccagta
     4261 gcatccatta aagtaatccg ttcgttcgac tgatggacga attcttccaa agcagttttg
     4321 ttttgcgctg cttggaggtt ttcgacatct aattgccgga tcagcagttg tgcttttttt
     4381 gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa aataatggga aatcaattgc
     4441 cagccaccaa aaaacaaact aactaaaaga ataagaaaga agagacgttc ataaattttt
     4501 ttccatttca tcgtttgggt tcctgaaact tatagccaaa tcctctaata gtcaccaagt
     4561 actttgggtg ttttggatct cgctcgattt tttctcgcaa atgactaacg tgtacatcta
     4621 cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt
     4681 cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta
     4741 gttcaatcag atggtcgtct acggtcactt gataattggt caaatcagca gtgatttgtc
     4801 caatcgatag atattcaggc tcagcttcat cttttgattg cttacgaggt tcaattcgtc
     4861 tgaatattgc tttcatacgg gctaatactt cgcgaggact aaatggtttg gtcaaatagt
     4921 cgtcagcacc tatttctagg ccaatgatcc gatccacttg gtcatcttta gccgtcagca
     4981 tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg agtgatctcc atgccatcca
     5041 tcgttggaag catcacatct aaaatgatga aatcaaatga ttgttctaat gctaattcta
     5101 atccttcgcc tccatctgtg gcactggtta ccttgtagcc ttctttttcc aaattgaacg
     5161 tcaagagcgt tacaatagaa ggttcatcat caacaactag tatttttttc attcaaatct
     5221 cctttaaata ggtttcattc tgtttatatt ttagcataga atcagtacaa agggctattt
     5281 tacaaaagaa ataaatggta taataggaag gcactttgct gaatagaaag gaagagcagt
     5341 caatgattgg aatcagtgca tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa
     5401 aagaagtacc agaattgatg gaactagttg aaactggaga agctgtcatg gtctgtccag
     5461 aagtgatcgg cggattacca attcctagag atcccgctga aatcattggt ggaaatggat
     5521 tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat gtaacggaag
     5581 cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac attgatctag
     5641 tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc gatttatgac ggaagttttt
     5701 ccggaataaa aaaaaatggt gccggagtgg ctactgctta ttttattcag caaaaaatga
     5761 ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag
     5821 accaatcgaa tgaacgcgct ctttgaattt tattcgacct tattgacaga aaaacaaatg
     5881 aactacatgg aattatatta tgcagatgat ttttcattag gtgagatcgc ggaagaatac
     5941 gaagtaagcc gacaagcagt atatgacaat atcaagcgga caagcaagat cttggaggat
     6001 tatgaaaaaa aactccatct tttttctaat tatattgttc gagaacaggt attggaaaat
     6061 atgactgcat atattacaga taagtacccg gaagataaaa aactacttga atatgtacag
     6121 caaattcaag caatcgaaga ataaataaaa ggaatgaaga aaatatggca tttgaaagct
     6181 taactgaacg tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag
     6241 ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt actagaagcc gatgttaact
     6301 tgcaagtagt caaagatttt actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc
     6361 tggaaagttt atcacctgca caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca
     6421 cacttggttc tgaaacagca gagttaaata aatcaccaaa aataccaact gttatcatga
     6481 tggctggtct tcaaggggca ggtaaaacga cctttactgg taaattagcc aattatttaa
     6541 agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga ccagctgcga
     6601 tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc tgtatttgat atgggaactg
     6661 aagtcagccc ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg
     6721 actatgtatt gatcgatacg gcaggacgtc tgcatatcga tgaaacattg atggatgagt
     6781 tgaaacaaat caaagaattg acgcaaccaa atgagatttt gcttgttgtc gatgccatga
     6841 ctggtcaaga tgccgtcaac gtagctgata gcttcaacca gcaattaggt attacagggg
     6901 tcgtcatcac taaacttgat ggggatactc gaggcggggc agcactatcg atccgctcag
     6961 tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa attgactgac ttagaagtat
     7021 tccacccaga tcgtatggct agccgtatcc ttggtatggg ggatatgctg acattgattg
     7081 aaaaagcgca gcaagactac gacgagaaaa aagcggaaga acttgcacag aaaatgcggg
     7141 aaaattcatt cgatttcaat gactttattg aacagcttga tcaagtgatg ggtatgggcc
     7201 cactggaaga tctaatcaaa atgatcccag gaatgaatca agtccctgga atcgaaaacg
     7261 tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat ggtttattct atgacgccag
     7321 cagaaagagc aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt
     7381 ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa
     7441 tgatgcagca aatgtccaaa ggggatatga acatcccagg tatggaccaa atgtttggtt
     7501 caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga
     7561 agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt cggtacagac tgaaaatgtc
     7621 tactgccgga gcttttctat cagcaatttt tattctttac taatacaatt actctaaacg
     7681 aagaggtggc acagatgaga tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc
     7741 atgatactgt ctggggtgtt cctgaatatg atgaccaaaa gctgttccga aaattgatgc
     7801 ttgatattaa tcaagccgga ttgagctggc agacgatttt aaataaaagt gaagcattcg
     7861 atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca
     7921 gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc
     7981 aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg cagtttttca gagtatcttt
     8041 ggtcgttttc taatggaaaa atcgttgatt cttcttatca ttcacaagaa gaagtcccaa
     8101 caacaagtcc tctttcagat gaattagcaa aagatctaaa gaaaagagga tttaaattta
     8161 tcggaagcac aacaatctat gcttttttag aagctgtcgg gatcatcaat gatcaccttg
     8221 acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc
     8281 gaaaaatcat attttatggt gctataagtt tagatggcta tttggcaacg aaaacggatg
     8341 atcttcaatg gctttttgac actccaacag gtaagcaaac tacttatgaa gctttttatg
     8401 aaacaatcga tacgaccatc atgggcagaa aaacttatca ggaagcaaaa aaatatttgg
     8461 agaccgaagc aatttatcca gagaaaaaga atttcgtttt ttccacccac gcacaattac
     8521 aattgccaga tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa
     8581 tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg
     8641 aacatcaaat gattgatgaa tggtatatcc agattacccc tgtattatta ggggacggga
     8701 ttcctttatt ccaaaaagga acatatgaag cgcgttttga aatgctagac acgacacgat
     8761 tcggtgaatt tatcgaattg cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc
     8821 aactatatgc agaaatgtcc tgtacatagt tgcctttttt attaccgtta tgctttttac
     8881 tctgttaatt tgttagaaaa atctagatac gcatctaaaa tcgtttggaa gaaacttaat
     8941 gttccttcaa cgatattacc attttcatca attagattcg caacgtttcc gatatatgct
     9001 tccggttgct gcaaggttgg cacattcaaa aagacaagtg attggcgtag atggtggttt
     9061 gcgccaaagc cgccgatccc acctggtgat gcagtaacga ctaaacctgg tttatgatcc
     9121 caaacactgt gtccataagg tcttgagcct acatctaaag catttttcaa tactgctggc
     9181 acagaacggt tgtattcagg agtaacgaaa acgacaccgt ctagtcccct cacttcctca
     9241 cgaaaacgag tccattcagc aggagcctgt tctggagttt cgagatcctc attgtaaaat
     9301 ggcagatcat cgatttttac aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa
     9361 gcttctgcta cttttttgtt gtaagaatct tttcttaaac taccaataaa aaatccaatt
     9421 ttttttgtca tacgattttt cctccttcaa actatctata ataatcttac aaaagataag
     9481 taactggagc aaataaaaac acttactgac aaaagctaca tagaagatag attacttatt
     9541 tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc
     9601 tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt acactacatc ttgtgaataa
     9661 aacaatggag gtggaaataa ataatggcag ttaaaatccg tttaaaacgt atgggttcta
     9721 aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt
     9781 tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc tgcagaagta gttttaaaag
     9841 aagacttagt tcttgactgg ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca
     9901 tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc taaattctca aagaaataag
     9961 gtgaccggat atgaaagatt taagcgactt agtcttaaca atcgttcgtc cgctagtcac
    10021 ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa gacttttacg agtacaatct
    10081 aacagtggct cctgaagata ttggccgtat cattggaaaa caagggcgtg ttgctaaagc
    10141 aatccgcaca atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat
    10201 catcgatgat aaggaaaagt aaataaacgt gctgtactcg aattttcgag tgcagctttt
    10261 ttgttttttt tattaattga agaaatgatt atttggcaaa acaagcaaga aagcgtatcc
    10321 ttataggagg agggagtgaa cagccaatga gaaagaaaat gttatttatt ccatttgctc
    10381 tattgttaag cgcttgtagt tcggttactc aagaatcaga caatacacat ctatcaagca
    10441 gtatatctga aactgttacc agtccagaaa aaagtagtac caaaacggct tccaccactg
    10501 aacaaacaat ggctacctca aataatgaga aaaagactgc tttagaccag ttgaaagaac
    10561 aacagccaaa tgttcccatg cctctagatg taccagtctc ttcaggctat ctcaatatcg
    10621 cagcgacaca tacaaagcaa ggttattcta ttttatatta cagaacagat cgtcccctcg
    10681 ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc aacctatcta tatcaatatg
    10741 ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc atttgagatc gacacaaatg
    10801 gtcagcaagt ggacttaggt tctcgaatca cagggtatca gcaaggtgca gcaggttcaa
    10861 gttttcttga atggcaagaa ggaaactggc gtattcgaat ccgtgggaat aatatagaag
    10921 gccaagatcc cttactatta gctaaagaaa tcgtagctta tttggaagaa aacagtctac
    10981 cagcacctga acaatttggt aaaattactg ttgatatggg tgatacgacg aatcgagccg
    11041 tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa gatcctatgt
    11101 ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc
    11161 catcatttaa ctccaagtca caggaagtaa tcaaaaatag cacgccgcat ttttgcataa
    11221 ttttggctta ataccttaaa agaaaactag aattgcctct tcacacatgc taaaataaaa
    11281 cagacaaaaa agaaatgggg atataagttg actgaatact tgaatgtagg aaaaatcgta
    11341 aacacacaag gtatcaaagg cgaagtccgt gttatctcaa caacggattt cccagaagaa
    11401 cgttacaaaa aaggaacggt gctaacacta ttccaagacg gaaaagcacc tgtggaatta
    11461 acagtcaaaa gtcatcgcaa acataaaaat tttgacttat tgagttttga aggacatcct
    11521 tctatcaatg atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa agacaactct
    11581 gtagatctag ctgaaaatga attctattac catgagatca tcggtctgca tgtattagaa
    11641 gatggaactg agttaggtaa agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg
    11701 gtcgttcaac gtccgaaaaa agctgatata ttgcttccat atatcgcttc tgtcgtcaaa
    11761 gaagtcgatt tagaagctgg agtggtccgt gtagaaattc cagaaggact gattgacgat
    11821 gaagattgat gtattgactt tatttccaag aatgtttgaa ggtcctatgg gggaatccat
    11881 catcggtaag gctgtagata aaggattatt agatatcaat atctctaact tccgcgatta
    11941 ttcagataac aaacatcaga cagtagatga ttatccatac ggcggtggag caggtatgct
    12001 gctcaaagtc cagcccatct atgataacat ccaagccatt gaaaaagctg caccggatgt
    12061 caaaaaaaga gtaattcttc ttgacccagc tggtaaacga ttcgatcaga aaatggcaga
    12121 agaattttca caagaagaac acttagtatt catttgtggt cattatgagg gatatgatga
    12181 acgtatccgc acattagtga cagatgaagt ttccttagga gattatgtcc ttactggggg
    12241 agaattaggt gcgatggtca tgatcgatgc aacagttcgg cttttgccag atgtgttagg
    12301 gaatcagact tcagctcaaa cggattctta ttcaaccggt ttattggaac atccccaata
    12361 tactcgcccg gcagaatata aagggatgaa agtaccagaa gtattgacaa atggaaatca
    12421 taaattgatt gaagaatggc aattaaaaga atcactaaga aggacctatc aacgtcggcc
    12481 tgatcttttg gaaacacttg aatggacacc tcaaatgacg aaatttttag aagaaatcaa
    12541 gaaagaagaa caagaaaaaa cagcagatga agccaagtga ctttttctaa aatcttcttt
    12601 acagtaggtt gataatatgt tagtatttta tggtgagtgc aaaagcactt aactattacg
    12661 atattccgct gtgagaaaag ctcataagaa tatttggaat aaggagaaga aaaccatgaa
    12721 tccattaatc gaagaattaa caaaagaaca attacgttct gacatcccag cgttccgccc
    12781 tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt
    12841 atttgaaggt gttgttatca aacgccgcgg tgctggaatc agcgaaactt atacagtacg
    12901 taaagtttct aacggagtgg gcgttgaacg tacattccca ttgcatacac cacgtgttgc
    12961 aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca aaattgtact acctacgtgc
    13021 attacacgga aaagctgctc gtatcaaaga aatccgtcgt taatcattgt tatcagactg
    13081 agaaagaacc cttggtattc aagggttctt ttttcttagc atgtaatttg aacaccctga
    13141 tccagttttt tctttaatta ttggtagggg agaataatgg attagaataa atctacctat
    13201 aagtagtagt aaaaaaaaga gcactatgat aatatctttc ttaagagtaa ccgctttaat
    13261 aaaaaatcca gaatattcta acataaatgg agtattctta gaatcaaaag gagaaagaag
    13321 atggtttata cttatacgga gcaagaaaag atgaaagttt tagacacatt catgaatgat
    13381 ggaaaagtaa aacaagtacc aagttcaaca aaaaagaaat acatcctatt gaaagaagtc
    13441 ttgaaacgct ttgaccatgg agtgacttat acagaaacag aggttaattc gatactgctg
    13501 aacgtatttt ctagtggcga ttatgtcgaa caaagacgtt atctaatcac atttggcttt
    13561 ttcaaaagaa gttcagacgg tcgggcatat cagatgatgg gaattgaaaa ttagaagttt
    13621 taatttatac tcattgaaaa aagttcaagt atgcgatatt ggttctacac cgcatacttg
    13681 aacttttttt gtcatctgta tctataatac aaagccaaga acgttattca aaatatggat
    13741 agctatgata aaagctaatg agcgattcgc agttaaataa gcaatcgtca gtaaaatcgt
    13801 tccaggagcg tacatgaaaa aatcaattgg atgcgttaca tgaataaaga taattctctt
    13861 ttatttgtac gaataaatac caaaacgtac ctcctaaaat tagattttgg acagatgccc
    13921 tttaaaaaga taacaagatt tcgttcttga aacaatatat atgcttttat tccaatgagg
    13981 ttgtgaaaac agtattttgt cctgagcgtt agaaaattga aaatggggaa tagcaactcg
    14041 tactttacca ttttttaagt gaatacaaga tcagctaatt gaacaccgtt tattcgtgat
    14101 gagggacaca acactgcagt cgacacattt ctcttttatg ttcaaagata acaaatattt
    14161 aaaacaaaga aaaatagtag ataatcccgt cctaactctt ttattcttcc ctcatagctt
    14221 ccaaatttaa gcaatacttc aattaatcaa gttttatctt tttttttcaa taatcgtaaa
    14281 aaaagagtat tttttattta aaaataaaat ctttgtatta aaggacaaga ctaaaataac
    14341 cttagaataa aaagagaaaa a
//
LOCUS       DACEKG010000073        14359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEKG010000073 DACEKG010000000
VERSION     DACEKG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.076"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(117..1367)
                     /locus_tag="IW664_002146"
     CDS             complement(117..1367)
                     /locus_tag="IW664_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0849496.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
     gene            1777..4149
                     /locus_tag="IW664_002147"
     CDS             1777..4149
                     /locus_tag="IW664_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849497.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            4210..5679
                     /locus_tag="IW664_002148"
     CDS             4210..5679
                     /locus_tag="IW664_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ0849498.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            5690..7699
                     /gene="topB"
                     /locus_tag="IW664_002149"
     CDS             5690..7699
                     /gene="topB"
                     /locus_tag="IW664_002149"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0849499.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            8045..8641
                     /locus_tag="IW664_002150"
     CDS             8045..8641
                     /locus_tag="IW664_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0849500.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            8654..9553
                     /locus_tag="IW664_002151"
     CDS             8654..9553
                     /locus_tag="IW664_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0849501.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            9556..9687
                     /gene="ssb"
                     /locus_tag="IW664_002152"
     CDS             9556..9687
                     /gene="ssb"
                     /locus_tag="IW664_002152"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0849502.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            9709..10041
                     /gene="ssb"
                     /locus_tag="IW664_002153"
     CDS             9709..10041
                     /gene="ssb"
                     /locus_tag="IW664_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0849503.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            10086..10319
                     /locus_tag="IW664_002154"
     CDS             10086..10319
                     /locus_tag="IW664_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849504.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(10478..10600)
                     /locus_tag="IW664_002155"
     CDS             complement(10478..10600)
                     /locus_tag="IW664_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0849505.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            10790..11014
                     /locus_tag="IW664_002156"
     CDS             10790..11014
                     /locus_tag="IW664_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849506.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            11457..11663
                     /locus_tag="IW664_002157"
     CDS             11457..11663
                     /locus_tag="IW664_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0849507.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            11663..11914
                     /locus_tag="IW664_002158"
     CDS             11663..11914
                     /locus_tag="IW664_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849508.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            12095..12367
                     /locus_tag="IW664_002159"
     CDS             12095..12367
                     /locus_tag="IW664_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849509.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            12812..12991
                     /locus_tag="IW664_002160"
     CDS             12812..12991
                     /locus_tag="IW664_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849510.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            13050..13406
                     /locus_tag="IW664_002161"
     CDS             13050..13406
                     /locus_tag="IW664_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849511.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         5177 a         2380 c         2964 g         3838 t
ORIGIN      
        1 gatgaaattt aagattttct ttattgagta atatgttttg aagcgcacga aactagaccc
       61 gatcaaaatc aaattttttt cgggttgatt caaggatttg cgtcaataac ggattgttat
      121 ttctctatca cactccttgg tggcgtatag agttggtgtt tacgtagtag cgtatccacc
      181 agacgcgtaa attttcttgc ggttaggacg agtgcacgtt tgtgttgatg ctttggaacc
      241 tccttatatt tactttgata aaaagctttg tattcaggaa gataattttt tacagagttg
      301 gcagcttcaa ctaagtagta tctaaaatat cgatttcctt gttttgtcag aggtgtattt
      361 tgcgactgat agtttccaga ttggttcact ttccaactta atccagcgta ttttgccaat
      421 tttgtctgat cttcaaatct ttcgatttga ccaatttcag cgattaaacc ggcagcatag
      481 actttaccaa cgcctggaat actggttaag cattgatatt ctggaatgac aacaattact
      541 tgttcgatgg ctttatctaa ttctttgata tttttttcaa gtgctcgtat ttctcgtacg
      601 agaacactta aaacaacatt aattgattct tgtgcaagag cacctaggcg gtaactcatg
      661 gtaattgcac gttgaattgc tttagcgatt ttttctggtt gcttgaaacg gccttttcct
      721 ttttcttgaa gtaaatcaca gaaagcttct aaaggaagct cagcaagttc atccaatgtg
      781 aaatcttccg tcatgagtga aatgagggtt gcactaaaaa gactcgtcga ttcgtctcgc
      841 atttctcgag caagcgtatt acatttgtac gttagatttt caagaaaatg ttgctttgta
      901 cgaatcaatt gcttaataag ttgatagcga gtacgtgtta aacgctgtaa ggccatgtat
      961 ttctcttctt tgatagggga agttgtgaat cgttgaatac gcaagaaatc agcgattctt
     1021 aatgcatcgt ttcgatcagt tttattctca tcaaacatgc gactaaattg tttcacccga
     1081 aaaggatttt caatcgttac aagtgtgttg agcttcttta gtttctcatc ttcatgaaaa
     1141 aacatggaag gatggaagct gtacatggac gttgactcca tgccaatcac aatttgatca
     1201 aagtggtagc tgtcattgaa ttcaagaatt gtttctcgta tgaatgaagc accttcgatg
     1261 tcattggcaa cagagatttc agataagata gacaattgat tgtcgtctgt taaaaaacaa
     1321 acatctagtt tttcagagct aacgtcaata ccgacaaata atttcatgga agaaggacct
     1381 cctttcgata taaattttgg aaaatgcttc gatactgtgg gatttcccag aaactcactg
     1441 tatagccaca acctcgccta atagaatacg gtgataaaag agctagaagc tgccttccaa
     1501 actactattt gaaagtctag tccattatcg aaacagctaa tgagttggta gtgataacag
     1561 tggttcgggt aacagactaa acagggtaga caaacgctac aggaggaaat agcaaattcc
     1621 cgttggatcc tttccataca tccattgtac tctgagaaat cacacaatat cgaaacaaat
     1681 tccgttagga atgtgttatt caataaattt tataatctag aaacattttg aataaaaact
     1741 attagacaaa tgatatttta cgaggaggaa acggacatgg cagaagaaaa agaaccacta
     1801 gacaatagtc gcttgggaaa atccaaaaga aaattagtac gattgcaaaa tgaattaaac
     1861 gaacaaattg agaagatgtt tgaacaccag agaaagacca acggtcagcc aatgaacgat
     1921 aagagaaatg ggcattcttg gtttaggcaa caagaacgtt tagaaaacaa agtgcatagt
     1981 ctgagagaag agatcaagca acaagaaaaa caggtagaaa aacttgaaag acaagaagag
     2041 ctaaaggaaa tgggctataa caagtacggc ggcctggata tgacaatcga aaatattcca
     2101 agaatcaaag aagaaatcga acgctttgaa aaaggtgaat cgactttttc agccgctacg
     2161 atccgaaaat accaacggaa attggaaaca ttagaacagc tgaaagaaag atcagaaaaa
     2221 ggaaaagaaa atcttcttcc tgaagtacaa gcaattattg acagcggacg agtgacgcaa
     2281 tggaaaaaaa atccaaccat ttattttttg aaagactatc gaaaagttgc tttagaactg
     2341 aatgaaaacg ggggatttga ggaatcaaaa aaataccaag ccacgtcgga agaggaaaaa
     2401 gcagctgttc gtgaattact tgaagaggct gcgccaactg attctttgaa ggagaaagaa
     2461 aacgatcatt tctatcgagt agaattcaac gaaaatgatc cagaaaaagg attgaaaagt
     2521 tatggtgggg agatcgtaac attagaattg atcgatcaac tcaaaacgtt ggataaccaa
     2581 tacgccgcag aagcaggtta ttataaattc tattttgaag aagtgaagaa cggagaagta
     2641 gtcgatcaga tccgaatgga tctaggggat ggcgtcaaca gtaatcaacc catctattta
     2701 gagttagcaa aattgtgcga acaagtacct gaaaaggaac aaaaacaaac ggtcaaaaat
     2761 gaacctgttt cgaaagaaca aatgcagtcg aaagaaacaa aaacaaaaca aaagggctat
     2821 caaaaacgaa cggcagaaga gattaagcaa gaagtcaaat ccctttcgca acaggcactg
     2881 gatgcggtga aaaagcatac ccattctcct gaagacgtga aagaactttt gaattttatg
     2941 tctcgttttc cagaacgatc ctttagaaat caattattga ttgaggaaca atttccaggt
     3001 gcaacggctt gtttaggtcg tgctgcgttg aaaaaagaag gaatctatat caaaaaaggg
     3061 gaacaaggga acaagatctt tgtcagaaaa acggtcaaag gattttatga gaaaggacgt
     3121 ggctttgttc gagaaacaga agctacacca gaagataaaa aacgaatcga aagtggtcaa
     3181 atcgaagtga tcaaaaaacc ttactacacg attgaaaaag tgtttgatat cacacaaaca
     3241 cagttaaaac ctgaagacta tccgaaaatc tttccgaacc gtgtctttga ttttcaattg
     3301 gataaaaaag gacagtcaga acttcaagct gggattcaag cggtagccaa caatattgga
     3361 atacaaattc gagatatgac agaaagtgaa gtctatcaaa gagaattagg acaagcacgg
     3421 ggggcgtatg ttcgtaacga atttactggc gaagaagaaa tcgtcatgaa tactcgaaat
     3481 actcctacgc agcatttagc tacaagtatt catgaattag cccatgcaag aatgcacaaa
     3541 ttttcagaat tagataccgc aactaaagaa ttgcaggcag aaatgacttc ttatattgtg
     3601 tgcaaacatt ttggaatgga cacttcggag aaagctattc cttatattgc agcatggacg
     3661 aagaatggtc agacgttgga tgataaggaa gcggcggaac gtgggaaaat catgaatgat
     3721 gtcagccgtg tcgcaaatga attcattcaa accatttcta atgaaatcaa tcaatacaga
     3781 gaaagagaac cagagatcca accagcagaa gtgaagcagg agcataaaaa tactgtagaa
     3841 acggcagaga ataagaatat tgtgcccgtt ggttcacttt ggatcgacaa gaaagacggg
     3901 aaagtaatgg aagaagatac gggtcgaacg ttacatttga aggatgcggc gtttattgaa
     3961 gatccgcagc ataatcaagt gattttaaaa ggaaaaagtt atggaaaaga gattcaaacg
     4021 gcgttacctt cagaagaatt tgcgctagga aatccattcc gattttcact tgaaacagtg
     4081 tcagaagtgg ccaaacgaaa aaatgaaatc tattatgaac aacaattgtc acaaaaagaa
     4141 cgcgtataaa acaagtaaaa aagactatct gaaaaacgga tagtcttttt tcttttggga
     4201 ggtttgaaaa tgaccgaaaa aaaaatgagt cttattgatc gatgtaaaca gatcgatata
     4261 gtggattttg ctcgaaacaa tggaatggct gtagtcaata aaggacgaga ctatcgctta
     4321 gaagaccatg attcatttgt ttttgatcga agaaaacaac gtttttattg gaacagtcaa
     4381 aatattagtg gcgacattat tgaattagct aaactttttt tcatagacaa agagatccaa
     4441 gactctaagc aacaatttaa agcagccaca gattttattc taaagaatga agacaaaaca
     4501 gaacgagtag aaaatcttca ttttgagaca gaaaagtaca aagatcaccc cgttgattat
     4561 caaccactca cagaaaaagg aaggaattat ctgaaggaag aacgaaaact accagagtgg
     4621 ctgattgatt atgcagaaaa agaagggttg attgctgaac taaagccgaa acatgaacgg
     4681 caaaattttt tagttggaga cgaccgttta gatcatgcgg ttgctttttt atggaaagat
     4741 ccacagacag gagagacagt aggggcaagt tatcaaggaa cgatcgtcga ttttaatcgt
     4801 ttcggtaaac ggggaacgta taaacacatt gacaagaatc ctacgccaaa tcacggattt
     4861 aatttaaaaa taggcgatcc gaagcattta aaattctttg aaagtagtat tgatctgtta
     4921 agttacgctg cattgaatcg ggagaaacta caagatgcct ggttagtttc gatggacggg
     4981 ttaaaacatc atgtaatcag tcattacgtt gaagagtcga tttctgaatt aagacgaaaa
     5041 caaacatttc ctcaatcgat tgaaatatgt gtggacaatg atcgtgcagg acatattttt
     5101 tatgaaaaag aacagatgaa gggaatcgtt gatcctttta ccaataagaa aatccgttgt
     5161 gagcgaggaa ttccaaatga ttggcaagta cctaaagaat acaaagctac ttatgaagca
     5221 gtcgcaaaag aaatgagtgt tgaacctgaa gcaattatgg ccatacacaa aactgaaact
     5281 aatttacagc tgacaaatca attggtgtct gctcatgatg ttcaatctac gtttggaaag
     5341 atgttggcga agggcgaacc agtagaaaca attgatttga aagaagcatg taccacggtc
     5401 gcgaaggaac tgaaagtatg tgaacgcgca gatggaacat acaactttga tcgtttttat
     5461 agtagaaaag ccaacataaa agacgttaat gcaggaatcc ttctttccta taaagcagaa
     5521 caatactaca aaggctataa aaagcatgaa catgaatttg tacctgaagt gaaaaaggat
     5581 tggaatgatc aattgaagca tgagatccag caacaagaaa tcagaaagca aaaacgtgcg
     5641 atgttgtttc aacaaggcag acaacaagaa agggagtgaa gtcgtcacta tgaaaaccgt
     5701 cattttagca gaaaaaccgt cacaagcgaa ggcttatgcc gatagctttt ctaaagctac
     5761 ccgaaaagat gggtattttg agatccaaga tcgattgttt acaggagaaa ctgtgatcac
     5821 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaaatggaa
     5881 acaatgggca ttagaacatt tgcctatttt ccccaatcaa taccactatc atgttcctaa
     5941 agataagaaa aagcagttta gtgtcgtcaa acgacagctg cagtcagctg atacgattgt
     6001 gatcgcgact gatagcgatc gtgaagggga gctgattgcc tggtcaatca ttcaacaagc
     6061 aggcgccaat caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc
     6121 catttatcag ggattccaac agttacgaga cgcagaagag acctatccaa aatttgaaga
     6181 agtacaagca cgtcagatcg ccgattggtt aattgggatg aacggtagcc cactgtatag
     6241 tttactcttg cagcaaaaag gcataccagg aagtttttct ttaggtagag tccaaacccc
     6301 aacactctat atgatttatc aattgcagga aaagattaga aacttccaaa aagaaccata
     6361 ttttgaagga aaagcccaag taatcgctca aaatggcgca tttgatgcga agcttgatcc
     6421 aaatgaaacc caagcgacac aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca
     6481 attaggaaaa cagccaggta cgatccttca agtcgaaaca gagaaaaaaa gtgcggctag
     6541 cccacgtctt ttttctttat cgagccttca gtctaaaatg aatcaactca tgaaagctag
     6601 tgccaaagat actttggaag ccatgcaagg gttgtatgaa ggaaaatatc taagctatcc
     6661 gcgaacggat accccttata ttacggaagg ggaatatgcg tatctgttgg atcacttaga
     6721 cgaatacaag cattttttaa aagccgaagc gattccgact cctattcata caccgaatag
     6781 tcggtatgtg aataataaga aagtccagga acattacgcg atcattccta caaaaacggt
     6841 tatgacagca gcggcttttg aacaactaag tccactgcag caggctattt atgaacaagt
     6901 gttaaaaaca acggtggcca tgtttgcgga aaagtatacc tatgaagaaa caacgatctt
     6961 gacgcaagtt cagcagcttc agttaaaggc aatcgaaaag gtgccgctag atctaggttg
     7021 gaagaagtta tttggaaaag agagcgacgg aaaagagaag gaagaagagc cgctacttcc
     7081 taaagtgaca aaaggagaaa tggtgacggt ggatctacaa gttcttgaaa aagaaaccaa
     7141 gccaccacaa ccttataccg aaggtacgtt gatcactgca atgaaaacag ctgggaaaac
     7201 cgtggatagt gaagaagcgc aatccatatt gaaagaagtg gaaggaatcg gtacagaagc
     7261 gacacgggcg aatattattg aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa
     7321 taagctcgtg gtgacgaata aagggatttt gctttgccaa gccgtggaaa aagagccgct
     7381 gcttacgagc gccgaaatga ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg
     7441 aaaaggaaca caaaccactt ttctagccaa tatccaaaaa tttgtctctc acttattgga
     7501 agtcgtacca gggcaaatcc aatccactga ttttggatcg acgttacaag aagtgaaagc
     7561 agccagtgaa aagcaagaag ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca
     7621 agaagtctta ctctatcaaa aggtggcagc ttgtacgtct gaagcctgtg attttaaaag
     7681 gtcagaaggg ttcatttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg
     7741 aattttctga aaagaaaaaa acgaacttca agaaaagaac acgccgcact gcaaaataat
     7801 ggagggttaa tcttatgcgt aataccacac acaaacaaca agaaatcgaa aatcaaaaag
     7861 ccaaaattcg ttcagaacag gaaaaaatgc gtgccagcaa acgaagactg cagcaattga
     7921 agaaccagca gaggattcaa aaagaaatcg aattagatcg ataaaatctt cctaactatt
     7981 gtatacgtat aaacttatgt tatactgttt ttataagttt atacgtatac aaaggaggga
     8041 gaaaatgaca aaagcaatgg tatacaagct gctagaagaa tttaatggaa ctttggcttt
     8101 gaaggatgcc aaaaaagcgg gcatttctcc tgtaacgatc aaacgtatgg tagatcgagg
     8161 ggagctggat cgagagtatc caggtttttt caccttacct ggtcaatttc ctgatgaatt
     8221 gtttatggcg caaaaaaaat atgaacgcgg cattatttca catattaccg cgttagattt
     8281 gtatgattta acagatatga tccctagaca gatcgatctg actgtacctt acggttatca
     8341 cgtttctgaa aaaggattga aggaattcgc agttgagcta cactatacaa aagcggaatg
     8401 gtatgaatta ggtaagattg aaataaaaag tcgctatggg aatcctatta tagcttatga
     8461 tccagaaaaa accttgtgtg acatttggaa tccctggtat aacgttgagg atgaaatcaa
     8521 agtaaaagca atcaaaaact acatggagtc ggacagaaaa aatcttagaa aattgaatga
     8581 gtatcgacga attttgccta cagataaaac gatgcgctct tatattatgg ctttaaatta
     8641 aaggagaata cacatgacac ctacgcaatt aaaagcaaaa gtgaagaata tttctagaga
     8701 aaaagaagtt gatccgcagc ttgttttaag acattttatg atggaaaaat tcttggaaaa
     8761 aatctcagag tctccttatc gtgaaaattt tgtgttaaaa ggcggcttct taattggctc
     8821 aaagtatggc attgaaaatc gaacaactaa agacattgat acgactttac gggaaatgaa
     8881 agtaaccaaa gaaacattga caacggttct aaacgatatt ttttcaacgc cgacgaaaga
     8941 aggcattcaa tttgagattc aaggaatgaa agaaacgagg gaagctgatt attatccagg
     9001 gtttagctta cgtgtactgg cacatttgga aaacatgcgg ccagatttta aggtagatgt
     9061 tactacaggt gactcgatct atcctgcgac gatcactcac agtcataaat tgatgtttga
     9121 ggatcgaacg attcctttgg aatcctatcc tacagaacaa attattgctg aaaaattaag
     9181 tgcaaccttt gattttttaa cagataattc gagaggaaaa gatttttatg atctctatac
     9241 tattcctaaa atggagaaga tcgatgagaa gaccttgaaa gatagtgtga gaaatacatt
     9301 tacacgtcga ggaaaaactg atccgttaaa gatatactat gaaaaggata tggatgtact
     9361 gaaaaatagt cacgagctaa aagatacctg gaagaaatat caaaaaagca atccttatgc
     9421 tgcgtcgatc acgtacgatg agacaatgga ttcgattagt aatttgatgg aaaaagtgat
     9481 tcacgtcgaa gaaaaagagc gaaaactagc ttatcaaaga caccaacaga tgaatagaga
     9541 aatggagaga taaagatgat taataatgtg acattagtag gacgcttaac caaagatcct
     9601 gatttacgtt ataccgcaag tggtactgca gtagcgactt ttaccctagc cgtcaaccga
     9661 aatttcacga atcaaaacgg caattgagaa gccgatttta ttaactgcgt gatttggcgc
     9721 aaaccagcag aaacaatggc gacattagct aaaaaaggta gcttgattgg tgtagttggg
     9781 cggattcaaa cgcggactta cgacaaccag caaggacaac gtgtatatgt gactgaagtt
     9841 gtagcagata actttcaact attagaaagt aaagcagcga cagagagccg tgcgcacgct
     9901 gatcaatcgt ccaccagtcc aagcaccgcc acctttgaac aacggaatac agcgacgcct
     9961 aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat cgatattagc
    10021 gatgatgatc taccgtttta gtttgagaaa gaaggagtaa gcatggcaaa aaaattagtc
    10081 tgacgatgga tctattgata cccgtaattg catttataga cgggatttct ttgggcgtca
    10141 ctgaaaatcc tttggtaaat agaattgcga aaggatcact atttcttgta ttcgtgtatc
    10201 ttgtctgttc ttatctttta aaaagagcag gaaaggataa ttccttttgg cgattgacta
    10261 aagtcacagt agcagcgaca gctgttatac ttacggctat tctaccgctt tatatgtaga
    10321 gttaacggga aatcgttgaa cttcttgcga aatattgcta taatgaacat gaaaaagagg
    10381 agttgcgcta acaacccctc attaacagca ccgattaaag aacggtggca gaatattagt
    10441 tatttggaaa taaccgctga cctgctaaag tagacggtta tttctttttg tcatttttaa
    10501 gcatatttac aatcaaacta atcaaagcaa tggtaaacat accaaaacca agaatggttt
    10561 gaattgtctc atatgcggac aaaaggctta ctcctttcat agattttagc acttacattc
    10621 ataagcacca ccacctttcc agaagtcgcc accgtcttca aactttactg tcttgactat
    10681 tatagcatat ctaaaatcga ataaaaattc tcaattttag ctgtttttgc tataatttta
    10741 gcgaggtgat cgtgatggaa caatatattg gtttactcga taaattaggg tgatcaagac
    10801 acagccattg cttgtgcgat ttacattacg cacgagtcat gaaccaatca attgtgtagt
    10861 ggccaatata gaaatagcgg ataaattgct gattttggaa gaaggcaagt atactatttc
    10921 agtgacaggt cacttcaata aacgaaagca actagtcatt gaatcaatgc aaattagaaa
    10981 ccctgatcat tttacgcgat cgatgagcat ataaaagaag agctgcagtc ctaaatgagg
    11041 attgtagctc tttttgtttt acatagaaac agtaaaaatg aaaaggcctg tttaggaagg
    11101 cacaagcgcc ttctcaattg aaagaccttt ctctcactcc aaccgccacc gccgttcgag
    11161 aagaaaggaa gtgaacgcta cgcgttcact aagataaagg gtcttgcata gcaagacaaa
    11221 atagaaaaaa tttcctctca ccaaaaggtt cgctcctgtt cgttatctct ttattgattt
    11281 gtcagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa gggtaaatgg
    11341 cacgctgcgc gcccttgtct tgtcgctttt cgttgattca ttctggccaa caaatcaata
    11401 aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa tcaatcatgc
    11461 gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac tatcatgcac
    11521 cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc tattgtttgt
    11581 atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattat attttaattg
    11641 atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgactg taaaagaagt
    11701 cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa gtagcagaag ttttacttat
    11761 tccgcggtac tggacgatag aaaccccttt agtagaggat gcaccgacta tgctatttga
    11821 agacctagtg aagattgcag gatatgaact gcattatcaa gaaaacttgg aaacttatga
    11881 aaacgaatat tggttaacca aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa
    11941 ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa tggaggatat
    12001 gacgttccta tacaacgaaa cacgtgttcc aaaaaaacat tataaagaaa aattgtcggt
    12061 agctgtggaa gaaatgattg aataaggtgt cgaaatgaat cttattgcga cgtactatcg
    12121 aacactggaa gaactgaaaa agcaaaatgc aaaatggttt ttccaagcct tactatgctt
    12181 ggaagtaggt gtcaaaccaa gcactattaa accttctgaa taccaagctt tagaactaac
    12241 gtatgcgaaa tttatcgaaa cgaaaaaagc caagactgtt tcaagtgaat ggttagatta
    12301 ttttgaaaat atcaataagt atggcgcata ttacaccatg aaaaaggaag acaacgaaaa
    12361 tgaataaggt gatttatcaa ctaacaacgg attccatatc aacgatttga aagaaaaaaa
    12421 ggtcaataat catttatacc gaaaagtgag aatgtgtgaa acaaggacaa aaaacgccta
    12481 gtaactaagg aaatggttct tatttcgcac caatcgctcc aaccgccccc gccgcttagg
    12541 tggtgcgaaa taaacgggca aagcccgata acttaagaga aagaaggtga tccatttgaa
    12601 caagattatt cccttattgt tagtactgtt tgttggcgaa atacttatgc gagatagtgc
    12661 agctgtacag ttaaacctcg ctgtattttt ggtaatactg gtgttttgtt tgatcttttg
    12721 gcggtttgta ttagtcctcg tgagtctact attcaagatg attgcagcca tatttttcat
    12781 agggttattt cgatgaaagg agtgagtacc aatgccaatt gcaataggaa ataaacggtt
    12841 acccgtgaca ttagatgaaa agagacaaaa agaattgcag caactaaagc agaagtatgg
    12901 caaaagtgaa tctaagatta tgtgtattgc gttagatcta ttgatcgcgc aagaaaaagc
    12961 aggatttgat gtaccagcgc taaaaaagtg acgtcacttt ttaatttgat ctatataagt
    13021 gatatcactt ttaccaagga gggaattaaa tgttaaaaac taaaaagaat tcgtacaaat
    13081 taaagcttag tatactatct attatttgtc ttttaggcat gataattagt ggtcatttgt
    13141 atagagtggc atatacgcag tttattgagt acaaggttca ctctttagga ttagctgggg
    13201 atcgatacgt tgaatatgtc cattattcat taaaatgttc ttcatatatt cttctttggc
    13261 ttattttttc attgtttgaa ttgctaaatt taatagcaat atcttcatat cacttagata
    13321 agctaaatac caaatctagc cgttataacg aagtgtttaa ggaaaatgtg aagttagaaa
    13381 gtgacttaaa caagctaaaa aaataaaaag aagaaaacat aagttaacta aaattatttt
    13441 attaagaatg atggaatata aggataaggt taccaacata acgatcatta cttctctgat
    13501 atgctatgga cttctatcaa tcagtatttt gtctatagat tatggagtaa ctatactgca
    13561 agccttagtt ttggctatgc ctatgtatct atctttgttt atcatgcatg aaatatataa
    13621 gctatttgtt ttatcaaaaa atgtagaaat acgggtaaat attttggtga atagttatca
    13681 aaaagaactc aaagaaagaa gacggtggcg gtatttattg aaaggatgaa aaagtaggat
    13741 ttgacatact ggtattcaga aagtgccgtc acttttaaaa caatggaaaa ggtggagata
    13801 agatgaagaa aaaacaaact aaactatcat tagcagatat ttaacacagt taaccgcaac
    13861 tgaagatggt gttgttgaat ttgaacaatc tgaaattgcg gtagtagatg atcgttacct
    13921 taaaatgcct tacttctttg accaagcaaa ggtcatttgc ctttgtcact acgatggggt
    13981 acgtgatttt aggcgttaaa aagtagtgat tatatcaact ttacgagtaa atataaattt
    14041 agacatgaaa ctgaattatc caatggaaaa tcatggttta taatctagaa aaattatagt
    14101 aatgattgga ttatatatat tctttcgtat tttcgtacaa ctgtagcttg tcaaaattga
    14161 gaaatttaac cgattaggta ctttacatcg ttatttgggt aatttctaaa acatgaagct
    14221 taccaacgaa taaactgaga ctatcttatt aattgatcaa tataagaaaa tatctttgag
    14281 gagatgatgt actatgtttt cgtctatcca cgattggtta ggtggtcttt tcggcggtgg
    14341 aggtaaaggt ttttaataa
//
LOCUS       DACEKG010000074        14161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEKG010000074 DACEKG010000000
VERSION     DACEKG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.077"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            66..767
                     /locus_tag="IW664_002162"
     CDS             66..767
                     /locus_tag="IW664_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0849512.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IW664_002163"
     CDS             764..1885
                     /locus_tag="IW664_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0849513.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IW664_002164"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IW664_002164"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0849514.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IW664_002165"
     CDS             3264..4172
                     /locus_tag="IW664_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0849515.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IW664_002166"
     CDS             4207..4479
                     /locus_tag="IW664_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0849516.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IW664_002167"
     CDS             4490..5536
                     /locus_tag="IW664_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0849517.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IW664_002168"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IW664_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0849518.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IW664_002169"
     CDS             complement(8547..9218)
                     /locus_tag="IW664_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849519.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IW664_002170"
     CDS             complement(9211..9705)
                     /locus_tag="IW664_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0849520.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IW664_002171"
     CDS             complement(9725..10945)
                     /locus_tag="IW664_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0849521.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IW664_002172"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IW664_002172"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0849522.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14148)
                     /locus_tag="IW664_002173"
     CDS             complement(13021..14148)
                     /locus_tag="IW664_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0849523.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4574 a         2603 c         2928 g         4056 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaaccatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatcttcg ccgtttgtat aattaagcga gacaaataaa aaagccattt atgttacact
    14161 c
//
LOCUS       DACEKG010000075        14026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEKG010000075 DACEKG010000000
VERSION     DACEKG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.078"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            140..460
                     /locus_tag="IW664_002174"
     CDS             140..460
                     /locus_tag="IW664_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849524.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IW664_002175"
     CDS             912..1112
                     /locus_tag="IW664_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0849525.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IW664_002176"
     CDS             1259..4048
                     /locus_tag="IW664_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0849526.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IW664_002177"
     CDS             4095..4622
                     /locus_tag="IW664_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0849527.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IW664_002178"
     CDS             4643..5833
                     /locus_tag="IW664_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0849528.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IW664_002179"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IW664_002179"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0849529.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IW664_002180"
     CDS             8224..8898
                     /locus_tag="IW664_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0849530.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IW664_002181"
     CDS             8895..9236
                     /locus_tag="IW664_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849531.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IW664_002182"
     CDS             9266..9625
                     /locus_tag="IW664_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0849532.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9828..10007
                     /locus_tag="IW664_002183"
                     /pseudo
     CDS             9828..10007
                     /locus_tag="IW664_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IW664_002184"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IW664_002184"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0849533.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="IW664_002185"
     CDS             12541..14007
                     /locus_tag="IW664_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0849534.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4649 a         2429 c         2998 g         3950 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttaca
//
LOCUS       DACEKG010000076        13977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEKG010000076 DACEKG010000000
VERSION     DACEKG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.079"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW664_002186"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW664_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0849535.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW664_002187"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW664_002187"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0849536.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IW664_002188"
     CDS             complement(2169..2906)
                     /locus_tag="IW664_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0849537.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IW664_002189"
     CDS             complement(2997..3524)
                     /locus_tag="IW664_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0849538.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IW664_002190"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IW664_002190"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0849539.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IW664_002191"
     CDS             complement(4401..4886)
                     /locus_tag="IW664_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0849540.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IW664_002192"
     CDS             complement(5039..5236)
                     /locus_tag="IW664_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849541.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW664_002193"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW664_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0849542.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW664_002194"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW664_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0849543.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IW664_002195"
     CDS             9211..9423
                     /locus_tag="IW664_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0849544.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IW664_002196"
     CDS             9425..11104
                     /locus_tag="IW664_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0849545.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IW664_002197"
     CDS             complement(11358..11558)
                     /locus_tag="IW664_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0849546.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IW664_002198"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IW664_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IW664_002199"
     tRNA            complement(12249..12322)
                     /locus_tag="IW664_002199"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="IW664_002200"
     CDS             complement(12634..13032)
                     /locus_tag="IW664_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849547.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="IW664_002201"
     CDS             complement(13025..13477)
                     /locus_tag="IW664_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849548.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13970)
                     /locus_tag="IW664_002202"
     CDS             complement(13464..13970)
                     /locus_tag="IW664_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849549.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
BASE COUNT         4116 a         2943 c         2348 g         4570 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagagt acaggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaaggtgtg gcaacaatcc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaatact
    13861 tgtaaaaaat tatctgtaag atgttcatct tcaaagatag tttcttcgag atatttaatc
    13921 gccaattcta aattgctatc attttgttga accaaccact taatgtccat ctcacta
//
LOCUS       DACEKG010000077        13917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEKG010000077 DACEKG010000000
VERSION     DACEKG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.080"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW664_002203"
     CDS             131..505
                     /locus_tag="IW664_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0849550.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW664_002204"
     CDS             complement(619..1248)
                     /locus_tag="IW664_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0849551.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IW664_002205"
     tRNA            complement(1382..1454)
                     /locus_tag="IW664_002205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IW664_002206"
     tRNA            1668..1741
                     /locus_tag="IW664_002206"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IW664_002207"
     CDS             1696..1845
                     /locus_tag="IW664_002207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849552.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IW664_002208"
     CDS             2338..3666
                     /locus_tag="IW664_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0849553.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IW664_002209"
     CDS             3868..4701
                     /locus_tag="IW664_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0849554.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IW664_002210"
     CDS             4717..5454
                     /locus_tag="IW664_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0849555.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IW664_002211"
     CDS             complement(5609..6577)
                     /locus_tag="IW664_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0849556.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IW664_002212"
     CDS             6809..7642
                     /locus_tag="IW664_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0849557.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IW664_002213"
     CDS             7676..8515
                     /locus_tag="IW664_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0849558.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IW664_002214"
     CDS             8541..9101
                     /locus_tag="IW664_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0849559.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IW664_002215"
     CDS             9300..11021
                     /locus_tag="IW664_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0849560.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IW664_002216"
     CDS             11186..12328
                     /locus_tag="IW664_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0849561.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IW664_002217"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IW664_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0849562.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4752 a         2298 c         2833 g         4034 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcaga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACEKG010000078        13335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEKG010000078 DACEKG010000000
VERSION     DACEKG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.081"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IW664_002218"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IW664_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0849563.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IW664_002219"
     CDS             complement(740..1462)
                     /locus_tag="IW664_002219"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0849564.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IW664_002220"
     CDS             complement(1598..2479)
                     /locus_tag="IW664_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0849565.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IW664_002221"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IW664_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0849566.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IW664_002222"
     CDS             3719..4198
                     /locus_tag="IW664_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0849567.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IW664_002223"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IW664_002223"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0849568.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="IW664_002224"
     CDS             5499..5627
                     /locus_tag="IW664_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0849569.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="IW664_002225"
     CDS             5631..6176
                     /locus_tag="IW664_002225"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0849570.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="IW664_002226"
     CDS             complement(6629..8320)
                     /locus_tag="IW664_002226"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0849571.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IW664_002227"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IW664_002227"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0849572.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IW664_002228"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IW664_002228"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0849573.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IW664_002229"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IW664_002229"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0849574.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12602..13303
                     /locus_tag="IW664_002230"
     CDS             12602..13303
                     /locus_tag="IW664_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0849575.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3752 a         2712 c         2367 g         4504 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    12241 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    12301 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    12361 attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt
    12421 ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat
    12481 aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact
    12541 ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt
    12601 aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga
    12661 acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa
    12721 aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa
    12781 aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt
    12841 gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac
    12901 agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt
    12961 agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga
    13021 aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca
    13081 aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat
    13141 tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc
    13201 tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt
    13261 tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc
    13321 agtgaccctg ataaa
//
LOCUS       DACEKG010000079        13181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEKG010000079 DACEKG010000000
VERSION     DACEKG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.082"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IW664_002231"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IW664_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0849576.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IW664_002232"
     CDS             1995..4661
                     /locus_tag="IW664_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0849577.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IW664_002233"
     CDS             5025..5777
                     /locus_tag="IW664_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0849578.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IW664_002234"
     CDS             complement(5799..6953)
                     /locus_tag="IW664_002234"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0849579.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IW664_002235"
     CDS             7138..9579
                     /locus_tag="IW664_002235"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0849580.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IW664_002236"
     CDS             complement(9909..10613)
                     /locus_tag="IW664_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849581.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IW664_002237"
     CDS             complement(10800..11045)
                     /locus_tag="IW664_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849582.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IW664_002238"
     CDS             complement(11062..11913)
                     /locus_tag="IW664_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0849583.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IW664_002239"
     CDS             12079..13083
                     /locus_tag="IW664_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0849584.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4209 a         2341 c         2753 g         3878 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc g
//
LOCUS       DACEKG010000080        13138 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEKG010000080 DACEKG010000000
VERSION     DACEKG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.083"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            20..397
                     /locus_tag="IW664_002240"
     CDS             20..397
                     /locus_tag="IW664_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849585.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1003..1617
                     /locus_tag="IW664_002241"
     CDS             1003..1617
                     /locus_tag="IW664_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0849586.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            2390..4129
                     /gene="yfcC"
                     /locus_tag="IW664_002242"
     CDS             2390..4129
                     /gene="yfcC"
                     /locus_tag="IW664_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0849587.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            4286..5470
                     /gene="iadA"
                     /locus_tag="IW664_002243"
     CDS             4286..5470
                     /gene="iadA"
                     /locus_tag="IW664_002243"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0849588.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5581..5784
                     /locus_tag="IW664_002244"
     CDS             5581..5784
                     /locus_tag="IW664_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0849589.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5885..6547)
                     /locus_tag="IW664_002245"
     CDS             complement(5885..6547)
                     /locus_tag="IW664_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849590.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(6560..8062)
                     /locus_tag="IW664_002246"
     CDS             complement(6560..8062)
                     /locus_tag="IW664_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0849591.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8610..10289
                     /locus_tag="IW664_002247"
     CDS             8610..10289
                     /locus_tag="IW664_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0849592.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            10416..10895
                     /locus_tag="IW664_002248"
     CDS             10416..10895
                     /locus_tag="IW664_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0849593.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            11114..11794
                     /locus_tag="IW664_002249"
     CDS             11114..11794
                     /locus_tag="IW664_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0849594.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11802..13133
                     /locus_tag="IW664_002250"
     CDS             11802..13133
                     /locus_tag="IW664_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0849595.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
BASE COUNT         4202 a         2258 c         2796 g         3882 t
ORIGIN      
        1 aaaaaggtgt gtgttcacta tgggaaaatt agtcgctgtt gcacagtcgc tgcttgatat
       61 actcaagtgg ctgatggaag gaagcaattg gatatttgtg ttgccactat tgcttattag
      121 tctatttatc atgtttcgca tggtcagaaa aaaagaaacg tttctgattt ttatctggta
      181 tgcagcatcc gttggggttg ctaccttgat ggttgaattg gcgttaggta gttgggaagc
      241 ttgcgtattt gctttatttt ttgctgtact gtatctaatc atttatatcc catatatgaa
      301 aagaaagtct cggataggca gaaaaggtag acatacaact actttttcta gtacagattc
      361 tctcgaacaa agtagaagaa cgagaagaag acggtaatat ttagtaggta tacgcaaaaa
      421 aagataaaat aatggataaa ctattgagat gagagattta tttaattggt ggaaaaaagc
      481 ttttttcact tatataaaag aatctttttt aaaggaatgc ttgttgtatc gggataagcc
      541 attccttttt ttatgttatt tatatatata taaatcatac caatgttttt ttgtattttc
      601 ttgcttgcac ttggataaga gattacttac aataaattca gatagaaaca gctgaataaa
      661 aaaaggctcc ctgaccctta gttggcgcta agaatcaggg aatgaccagt cacacacata
      721 caccctgacc gatcattttg ccatagtcaa tgcagtgcat cgacctaatt tattgtacct
      781 aatctagggt gaaaattcta cccctatgta gatattttcc atggtctgca cgttgtttta
      841 tttgctatgt caaggtattg ggcgtattcg ttcaatgcct tttttgtgtt tgaaacagca
      901 aagaccacat gtgttactgt ctaactactg tttttttatg tacttcctta gattaggcag
      961 ctgtttctat tcatgtcaag tatagctagg cgggagagat aaatgagttt atgttatttt
     1021 gactattatc accaattgga agagaaatta aggatagaag agccgctgat gcttcaagct
     1081 ggcacttgga tcgcgaaaca aattaatcaa ggtggtagat tgcagatata tgctagccgc
     1141 acactaagtg gtgtagcatt tgaattttgg gatcaaaccc cggatcttgt accaagtata
     1201 ttgattgaaa atccagccgg aggtgtctat gaaacgctag aaggtgcagg acaggcaatt
     1261 attgatcagc tttctgtcaa atcagaagac atttttcttc ttctttcgaa tgaaggaaga
     1321 aacccggcaa tcatcgaact agctcagtgg atcaaagaaa atggtcatcc attaatcatt
     1381 gttactggat ttgatttgtc acgaagtatc aaatcgcaac atagcagtgg tttgcgatta
     1441 tttgaatttg ctgatttggt tttggacaac cacgccaatt tacatgatgc agtcttgact
     1501 ttgccaaatc tagatccagc gatctgcgga acagcctctc ttgcaacgat ctttttgctg
     1561 cagcaagttt tgtattttac agcaaaacag ctagtccagg acacaagtgg aaaatagtta
     1621 tccacatgtt gacaaagttc cttaaaaaaa taatgttcca cataaaaatg gagcgtttga
     1681 aaaaaagaaa acgattatta atgaggatta acaacgacta ctttttttgt tttagttatt
     1741 ttaagatgga tcatctttga ttttaagctt gttttgttta aagtttgtta tttttataag
     1801 ttgtttcaca gttatgagaa atgagttata agaatatgta gtgtcagaaa aataaaaagt
     1861 agtggaaaaa gagacaagaa agaatccata tggattcttt cttgtctctt aatacacgtt
     1921 gatgaaagag tcttccccaa aactctttac aaagtaatta tagtacattt gttatcatta
     1981 ttgcaatgaa gtttacctga tataacaaaa ttgtccaaca ggataccttt ttctaataga
     2041 aataaaatac acaaaaattg aaggatagat aatggatatt tataagtgtt tattattttt
     2101 tttgcaatga aattcaattt ttcaatgatt gtaaaatgaa aaaaacagga cgagattttc
     2161 tctaatctaa aaataaaaaa agaattttaa ggaaaaacag agaaataaaa tacttaatat
     2221 aaatggaaag agaaaaaaat aatcatacat aggagaaaaa aaacaaaata gaaaaaagtg
     2281 attttctaat aaattattca tgtggaaatg tcatttttac tgtataattc atttgattat
     2341 ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac
     2401 aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt
     2461 cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa
     2521 gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt
     2581 tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca
     2641 attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg
     2701 attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat
     2761 ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa
     2821 aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt
     2881 aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc
     2941 tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga
     3001 cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct
     3061 attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc
     3121 attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt
     3181 aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag
     3241 cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt
     3301 gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa
     3361 aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca
     3421 tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt
     3481 agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat
     3541 tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct
     3601 tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc
     3661 tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg
     3721 aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc
     3781 gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt
     3841 caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc
     3901 actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc
     3961 aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc
     4021 acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt
     4081 tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc
     4141 tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt
     4201 aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt
     4261 atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc
     4321 agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga
     4381 acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt
     4441 actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg
     4501 ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac
     4561 agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa
     4621 agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt
     4681 gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat
     4741 cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt
     4801 cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat
     4861 caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga
     4921 aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt
     4981 tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga
     5041 tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt
     5101 gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt
     5161 gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt
     5221 gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg
     5281 agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat
     5341 tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat
     5401 cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa
     5461 aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct gtttgtctgt
     5521 atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg aggaagcaca
     5581 atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa
     5641 gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa agcacaatca
     5701 ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa agacaagttt
     5761 tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa gcaaataaaa
     5821 aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat cattatcctg
     5881 ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact tcatcacagc
     5941 tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt tcatgggcga
     6001 tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt ccagtaggct
     6061 catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg actcgctgct
     6121 gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca attcctacac
     6181 gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta gtgatttcca
     6241 ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa gcttggaaaa
     6301 taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt gaacgatctt
     6361 gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa agaaaagtcg
     6421 tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg aaattcagtt
     6481 ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat tgttctgtcc
     6541 gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat cttaatatcc
     6601 ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg gctaatagtc
     6661 ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa acagcaaatg
     6721 gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt ctgttacctg
     6781 gcatttgtcc accgccagga gcttgctggt tttgtgtcgt ggtttctgtc tgttggtcaa
     6841 gtagctgctg tcccacggca ttcgcgacga cgtttccgct aagcgaagca actcctaatg
     6901 caagaatcat acaaatagcc acttcggtaa acaattggag aatgatttta cttctggatt
     6961 ctcctaaaga tagcaagacg cctaactcat gccgacgctc ccgaatggta atcataatga
     7021 tcagagtaag tatgataata ccggctacag ccactagtaa aacaacattt ttagaaaaac
     7081 ttgccacgtt gtttaaaggt tccaacattg actgatacat ggaatcattt gattgtaagc
     7141 taaatgtatc tgtgtcgatc agcttctcag cttctttgag aaactcatcc attttatctg
     7201 gatcagatag agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc ccatttagtt
     7261 catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag ttgaatttca
     7321 tacccatact gccgcttgtt tcgctggatt cataaattcc tttgatcgtc atttcataag
     7381 tggtatcttc atcttcagta ctcgtgattg tgaaagtatc tccgactgcc aggtcatttg
     7441 tttctgctag agtagaatca atgactacat tgtttgtgcc tttgtcgctg tcatctattc
     7501 cttcaccatc cgtgattttt gctgttccat cagaaaaatt actattttgt gcagttgctg
     7561 acacaccact gatttgaaag tctgcttgag tcatttgcgg attcccgccc attcctccag
     7621 caggttcacc gcctgcatca gtagtttctg aactgtcttc acttgaatca gaactagtga
     7681 tggcagttat tccatctttt gccatagctg aggcggaact ttcaaaggaa taactcttta
     7741 cattatctaa ttcagctatt ttttccgcgt ccgttaagga aactggtgta agagaaaaac
     7801 tgcctggatc tggacgttct tcagtggtag aaccgcttga agaagtttgt tcctgttttt
     7861 taaatgaagc ttctcgattg gcacttaaag tcactgttgc tccaacttct ttttgtgctt
     7921 gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca aaaataagaa
     7981 tggctgtgaa cacggcaaac aataacaatg aacgtcccca ttttactttt gtacttttcc
     8041 atgcacgttt cataaaattc atattttgca tccctcctag tagatgcatt tgataataga
     8101 caacctatct taaagctagc ttaaatctta tgctgtttgc acaccatcaa catgaggttc
     8161 tgtatatata aaaagtcact atagttattg caaaaaatgt ggtaaaatga aaaaagtatg
     8221 tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa agaaatccta gatgctacag
     8281 agggattttt acgtgcatag ttgttctaac aattgctgat actagcgatt tagtttattt
     8341 gttgtataat aatggggaac agctgaatac ttatataagt attctggcta tagcgtttcg
     8401 caaaagatcc attttgttaa tgaagtatat ccaaaataaa atgcccatcc aactgtcaga
     8461 acgcggatgt gctggtaatt ttcctgctga ttctccataa gttgagaaat agtgattttg
     8521 catatgcttc attggaatgg ctcatatatt tagataaaga agaaaatcga catctttaaa
     8581 cttggatgtc ataagaagag gtgaagtttg tgagtaccat taaaattatc ccattaggcg
     8641 gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat atttttattt
     8701 tagattgtgg gctaaaatat cctgaaaatg agttgttggg gatcgacgtc gtaatccctg
     8761 attttacgta tttagaagaa aacattgatc gtgtagctgg tgtcttttta acacatggac
     8821 atgcagatgc gatcggggca cttccttact tgttgtcaaa aatttctgta ccagtattcg
     8881 ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa gcagccaaag
     8941 gatttaagga tttccatgtg gtagatgagc atacagaaat agactttggt gaaacagtag
     9001 tgagcttttt ccgtaccact catacgatac cagattcagt ggggatcaat ctgaaaacaa
     9061 aagaaggtag tatcgtttat acgggagatt ttaaatttga ccaaacggct atcccaatgt
     9121 atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga aggagtctta gccttactaa
     9181 gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa atagcagatg
     9241 aagtatttga tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt gtagcaagta
     9301 atttgcaacg agtgcaacaa gtattggatg cagcagaaaa agcacaacga aaagttgtct
     9361 tgactggtca agatttcgaa cgaattattc gcacagcgat gcgtttagaa aaacttcaat
     9421 taccaagtga agatctgctt gttacgttga aagacatgaa aaactatgat ccagaagaac
     9481 tgatcatttt agaaactggc cgaatgggtg aacccatcaa gtctctgcaa aaaatggcaa
     9541 acggtacaca ccgtaatgtc aagattgaag aaggagattt ggtttatatc actactacgc
     9601 caagcatcgc gatggaaacc atcgtggcga aaacggaaga tatcatttat cgagctggtg
     9661 gaacagtgaa gttgatctct gagaatatgc gcgtttctgg ccatgccaat cctaatgact
     9721 tgcaattaat gatcaatttg atgaaaccaa catattttgt gccagttcaa ggagagtatc
     9781 gtgaattagc tgctcatgca gatttggctc atgcagtagg gatgccatac aaaaatatct
     9841 atattactgg acgcggagac gtgctggaat ataagaacaa gcgtatgagt gttgcaggaa
     9901 ctacaagtgc agaaaatgta atgattgatg gattaggcat aggtgatatc ggaaacattg
     9961 ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt gtcactatca
    10021 gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac ttcacgggga ttcgtttacg
    10081 taaaagctag ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca atcgttgaga
    10141 aacatttaga aagcaatgat tttgagtgga gcaaattaaa gcaagacatc cgagatcaat
    10201 taggccgtta cttatttgaa caaacaaaac gccgcccagt gatcttgcca gtcattatgg
    10261 aagctactca acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc tctctataga
    10321 gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt ttgaatacag catccctcgc
    10381 tttataataa acgatagaat aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac
    10441 gttatttaga agaacaaaag atcagctatc aaccacttga ttttagtcat gtggtagaag
    10501 aatctttcac agaagaacta caaaaaagag ggattgatcc agctttgata tgtaaaactc
    10561 tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt gattcctttg aacgatcact
    10621 tagattacaa aaaaacgaga caagtgacga aagatcgtaa agttggtttt cctggaatgg
    10681 attttgtatt aatgcataca ggatatcccc acggagcaaa cacccctatt ggaataaaac
    10741 tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa gaagaagtaa
    10801 ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat ttagaaaaga
    10861 tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa gctaagcaga
    10921 gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct gtattatctg
    10981 ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt agcagctata
    11041 ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac agagaaaacg
    11101 cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa ttgaaatgga
    11161 aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga cagacttaac
    11221 tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg accacatagg
    11281 agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg ttgagattgc
    11341 tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg gaaaacatgc
    11401 gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct atgaagtaga
    11461 cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag aaggaaaaac
    11521 gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg cagaagaatt
    11581 cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc cagaagatgc
    11641 agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt
    11701 ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa
    11761 aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca aaacatgatt
    11821 tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta agaagcatcg
    11881 caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg
    11941 tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc aaattaaaaa
    12001 aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg gacgttttag
    12061 gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg atgactaaac
    12121 atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac cgggcggaag
    12181 tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga ttcaatacga
    12241 ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc acctcttatg
    12301 atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg atcaaaaaag
    12361 atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat ttgcagcaga
    12421 cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg cgttttccag
    12481 tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt ttagggaaat
    12541 cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa
    12601 tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc atgccggtcg
    12661 tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg aaagcgatgc
    12721 agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata tctggaaata
    12781 tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg
    12841 tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta cttagcgaaa
    12901 tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat atcttgattg
    12961 aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta gacattcgtc
    13021 cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg tttattgaaa
    13081 acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct tagaaagc
//
LOCUS       DACEKG010000081        12956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEKG010000081 DACEKG010000000
VERSION     DACEKG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.084"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            93..422
                     /locus_tag="IW664_002251"
     CDS             93..422
                     /locus_tag="IW664_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0849596.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="IW664_002252"
     CDS             412..699
                     /locus_tag="IW664_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0849597.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            985..1842
                     /locus_tag="IW664_002253"
     CDS             985..1842
                     /locus_tag="IW664_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0849598.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1843..2391
                     /locus_tag="IW664_002254"
     CDS             1843..2391
                     /locus_tag="IW664_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849599.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2688..3635)
                     /locus_tag="IW664_002255"
     CDS             complement(2688..3635)
                     /locus_tag="IW664_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ0849600.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            3823..5010
                     /locus_tag="IW664_002256"
     CDS             3823..5010
                     /locus_tag="IW664_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ0849601.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            4991..6295
                     /locus_tag="IW664_002257"
     CDS             4991..6295
                     /locus_tag="IW664_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0849602.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            6321..7334
                     /gene="citC"
                     /locus_tag="IW664_002258"
     CDS             6321..7334
                     /gene="citC"
                     /locus_tag="IW664_002258"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ0849603.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            7338..7631
                     /gene="citD"
                     /locus_tag="IW664_002259"
     CDS             7338..7631
                     /gene="citD"
                     /locus_tag="IW664_002259"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ0849604.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            7636..8541
                     /gene="citE"
                     /locus_tag="IW664_002260"
     CDS             7636..8541
                     /gene="citE"
                     /locus_tag="IW664_002260"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ0849605.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            8534..10093
                     /gene="citF"
                     /locus_tag="IW664_002261"
     CDS             8534..10093
                     /gene="citF"
                     /locus_tag="IW664_002261"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ0849606.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            10090..10647
                     /gene="citX"
                     /locus_tag="IW664_002262"
     CDS             10090..10647
                     /gene="citX"
                     /locus_tag="IW664_002262"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ0849607.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            10640..11503
                     /gene="citG"
                     /locus_tag="IW664_002263"
     CDS             10640..11503
                     /gene="citG"
                     /locus_tag="IW664_002263"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ0849608.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            11737..12146
                     /gene="tnpA"
                     /locus_tag="IW664_002264"
                     /pseudo
     CDS             11737..12146
                     /gene="tnpA"
                     /locus_tag="IW664_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(12783..>12956)
                     /locus_tag="IW664_002265"
     CDS             complement(12783..>12956)
                     /locus_tag="IW664_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0849609.1"
                     /translation="LKSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDE
                     WLSSSRKYIKFAK"
BASE COUNT         4599 a         1958 c         2544 g         3855 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
      721 aaggacggct tcttttttat cgaaaatctg attcgtctac atgactaaag tcgcgagtat
      781 tctctgcttc tcgataaatt aaataacgtc attactttga ggaaagctat aaatcaatat
      841 atttcaactt ttccttattt atgtactacc ttttacttat taattataag gtagtacata
      901 tatatctttt ttttggtagt aactaaaaat aaatatgtta taatagtatc aaatgaatat
      961 tgctaaaaga aaagaggtag gaacgtggta gcgaagaaat atgtaattgg taattttaaa
     1021 ggtggcgtag gtaaaagtac gtgcgcgcaa atgttagggt ttgaatcagc agttaatgaa
     1081 ggaaaaagaa ccttgataat tgacttggat atgcaaggaa atacttctga tgtgatgaac
     1141 ttaacgcata tgaacttcac taacgaagaa ggaggcggag aaggtgaacc gattgtttac
     1201 gaaaatactg tgactgacgt gttgataaac aatcttccac ctaaagaggc tgtctataaa
     1261 gtaatcaata acctctatat acttccagca gatatgtcct ttgaaatgta tgatgattgg
     1321 atcaaagacc aatataatga ttcattatcc caattcaaat atatgaaaga aaagctagaa
     1381 ccattatttg atgagtttga cgtcatctat ttagatgtcc caccttctat ttctgtatat
     1441 agtaaatccg cgatgtacat tgcggactgg gcaatcgtcg tcttacagac ccaagtaaaa
     1501 tcgatgagaa atgcgatgca atatcttgag tatatggact tttttgtgaa agaatttgat
     1561 acaaatttat atgttgctgg tgttattcct ttcatgttgg aaagcggtga cgcagtagat
     1621 caggaaatgt ataaacaagc ccaagagatt tatggggaac atttgattaa aaatgttgta
     1681 ctcaaaaatg cgcgcttaaa acgatacgac ggatcaggta ttacgatgga gaagacaaag
     1741 aaaggtctct taaaacaatg ggatcgcaaa agccacgaat tatttatcag tattttgaaa
     1801 gaattggaag aacacgagaa ttggtatgcg gaggtggagt aattggctga catcaaagga
     1861 cttgatattt ttgggaacaa caaaggactc gccaaaataa aaaaagaagc agaagcaaag
     1921 aaaaagcagc aagcaattac accatctgaa aataagcaat tagaagtgca ccaagttcaa
     1981 gaaataaaaa cttcctccaa tgttgaaaga cagagtagag gaagaacaaa aaaagttggc
     2041 gcaccaatca gaaagtttga taaggtttat tccaccaagc atcctttaaa actgtcgact
     2101 ttgttaaatg cgacttccag agttttagta gaaaagtatg agactaatat gacaagagat
     2161 gaattgctta gaaaagcttt agatgagtat attaaacaga atcttacgca agaagataag
     2221 caagatttgt ttaatgacgt ggtgaaagaa ctagacttgt tcagagaaaa atatccaatt
     2281 gttccagaat tagatgaccg tggagaaata ataagaacta ccagtcagat aaaacaagat
     2341 accgaagact cactgcgcaa agggtgggga atgaataata aagttagata gaaccccctt
     2401 tttatagcat aaaaaagatt tagaaggata tatgatccta ttgcatgaga agtacaataa
     2461 aattaataag aagtaacatt aacgaaaaaa cgtggaaatt attcagtgaa tacaaagaaa
     2521 ttaaatcttg aaaagtaatg gagtatggct tggaaaagtt caaaaaatat caagacaagc
     2581 ttctcaaaaa aagggatttt gatatccaat aaaaagagtg aatcattgta ctgccctcca
     2641 aaaaagtagt accaaaaatc taaatttttt ggaggatttt acatgactca ctcttttttt
     2701 atcaaagaaa gtattttcca agcagttttt tcatccaaaa ataaatctgt aaaaaaatta
     2761 ccattcaagg cgcctaaaca actctccgct ttgaacttac ttttacaaat accaattcgt
     2821 tttggaatat cgaaaatttc ttttaaagaa agaccaaaag tcttatcctc agatgctttg
     2881 atgaattcac cattgatatt gaagggtctt ccgtaaagaa atccaacagt atccgtcatg
     2941 cttggaaata ggattttacg atgcttgttc cagggttcga cagaatcaat agaggataag
     3001 gtgccaattc cagaaattaa catatctgca tgcgaagcgg ttgctaaggt attagctaaa
     3061 gaaggttcta gagccattag ttcacgtgca gtttcattga tgacatataa aggaatactg
     3121 agagtcaaat aagaagactc ataacgttca gcagcttttt gaactaatcg tgtggagcca
     3181 gctaaggaat tatattttcc gatttcgcca atgaattgag taaaaattaa ttcttcacga
     3241 gcacccattt taaactgatc aatcactttg taaacggtat ctccccaaga tagtgcaacc
     3301 acattggctt tcttaattaa ttcttgaatg taatcggcag caaattttga aaataataaa
     3361 tcgtgttgtg ataagtcttc actacttttt aatatataga tattcgtttt aaagtaattt
     3421 ttgaagattt cttctagctc ataattccta gcatacggag aattaattga aatactaact
     3481 aattgttttt cacgtgcatc ttcaatatat tttaaagctt tatatctggt gatcccatat
     3541 tttttagaaa tttcactaat attgatatcc tcgagataat agtcttcaca aatttttaca
     3601 aggagtgatt catagtcaga gaattccaga tgcatagtat agctcctaac tttttcaaat
     3661 agatattttt tctcattata ccaaaaaaat tgaatttagt ctttttttaa atttttaaaa
     3721 ataagcatca tttttttaaa atttaaaatg aaagggtttt tttattttaa aaaaagtgtt
     3781 aaagtggttt tgtaattaaa aaatgtaaaa aggagtgaag gtatgcttga tgaagtttta
     3841 gaaatccata aaaaaaatgt aggtgtttta cggatggaaa caacagaagc cgtgcaagat
     3901 gatcaagatt tagcaaaaat gtacacccca ggtgtagcag aactaagttt aatgattgag
     3961 aaaaatcatg ctttggcaag agaattaaca attagtggta aattagtggc cgttattaca
     4021 gacgggtcgg cggtattagg tcttggaaat gtaggaaacc aagctggatt accaatcgtc
     4081 gaagggaaag cgctgcttta taaaaattta gcaggtgtca atgcgattcc attagccatc
     4141 gaacaaaaat cagtagacga gattgtacaa acaattgtca atttgcaaaa tagttttgca
     4201 gggatccatt tagaagatat tgcagcaccg aagtgttttg agattgaaga aaaattacaa
     4261 gaaaaacttt ctattcctgt gtatcatgac gatcaagaag gaacagcgat agttgtttta
     4321 gctggattga tcaatgctgc taagatccaa aggaaaacat taactgaact tcgagttttg
     4381 atcaacggca tgggagcatc tggggttgca acagctagac tattaatcgc tgctggtatc
     4441 aaaaatctaa cattggtgga taaacaaggg agattaaaga agcaagatag tacattaaat
     4501 aaatatcaaa gagagttgtt ggatcatata catgatacaa ataataatgc aagcgttttg
     4561 gaagatgcaa tcgttggaca agatgttttt atcggtcttt caataggaaa tgttttaacg
     4621 cctgaaatga ttggaaaaat gaatgataat ccgattattt ttgctttagc caatccagta
     4681 ccagagatca cacccgattt agcaaaagaa tctggtgcaa agctaatcgc tacaggttct
     4741 tcgaaatatc ctaaccaagt aaacaatatt ttagtatttc ccggattgtt caagggacta
     4801 ttactatcga aagcgaataa agtcgatctt tcattacaac ttgaagtagc caaacaatta
     4861 gctgctaagg tcgcagagcc tacggtagaa aaattcatcc ctaatgtttt tgatgaagga
     4921 gtcgcaaacg tcgtttcaca ggcagttttg gattatttac aaaaactaaa agaaaacgag
     4981 gataatatac atgctgaaaa cattaaatag aattaaaatt ggttttattc ctttatctct
     5041 ttatatcatt ttggtactcg cgtatcttgc aatgacgata agcaatatcg ttccagaaaa
     5101 tgatatgtta gtttctttgg gtattatgac tgtttttgct tatttattag aagaaattgg
     5161 taaaactatt cctattttaa aagatttagg tggtaaagta ttagttgtta catttttgcc
     5221 tgcatttcta gtctataaac attggctacc tcaaagtact gtaactgtgg tttctgattt
     5281 tatgaataat aacaatttct tgatgttctt tatcacacta ctagttgtcg gcagtattgc
     5341 ttcaatgaat cggcaaacat tggttaaagc aacttcacgt attatcatta ttttaatttt
     5401 atgcgatgtg attggttctt tgataggaac aggtgtggga atactccttg ggctatctgc
     5461 ttggaatgcg tatttcttta tcgttgcgcc gattatggct ggtggtgttg gggaaggagc
     5521 gatgccatta tcgattggtt atgcagcaat attatctggt atgggacaaa atgatatctt
     5581 tgggaagatt ctaccgtgtg tgatgttggg aagtttagtc gcagttctac tgtctggtat
     5641 gctcaaaaaa attggagaga aatacccaca attaaatggt cagggtaatt tgatcgatgg
     5701 ggatgatgaa catttagaga acttgaaggg ttcagctaaa ccagtcaata ttgaaaaaac
     5761 attgttagca ggcgtggtag caataacgat ttattttgtt gctgtctatg ttgagcactt
     5821 attaaaatca ctccttggct tttctattcc ttcacctgtg atcttattag tctctcttat
     5881 gggggctaaa atgcttggtt ttatccctaa aaatatcgaa gaaggtggaa attcgttgta
     5941 tggattcact gttcgtggga tcacacctcc cttacttttt ggcgtagggg ttgcaaaaac
     6001 agattggtta gagctgatcg aagtatttaa aaatcctgca acgatagtaa caatcattgt
     6061 gacggtagta gctattgtcg ctacagcttt tatcgccgca aaatttttaa aaatgtaccc
     6121 agttgatact gccatcgcag tttcatgttg tagcggacaa ggcggtacag gtgctttagc
     6181 tatcttagct gcgggtgatc gcatggaatt aatgccattt gcacaagttg ctgttcgact
     6241 agggggtgct atgaccgtaa cgttcgctat ctttttaatg ggtctgctat cataagaaca
     6301 taaagaaagg acgtgaccat gtgtttgatc aaacagatat ccaacgatta tttttaaaaa
     6361 ataactatat caaagaccaa tgggaaaatc ttttgcttga ttcaggaata caaaagaagc
     6421 aaatcgaaga ttttcaacat ttagatcaaa cattgggtat ataccataaa gaaaagttag
     6481 taggaacagt atcttatcaa aacaacgtga ttaaatatat cgccgtgtct gaaaaatata
     6541 aagatggtgg acatacattt aatgtactag tctctgcaat aattcaggca atgagtcaac
     6601 aagagatttt tcattatatt gttttcacga aaccgcgcta tcaaaaaagt tttaactttt
     6661 taggttttga aacaatcgta tctaccccaa aaatagctgt attagaaatg ggagatagtt
     6721 ctataaaaca gtacctagag aggattccta aagtgaatgg tgaaaaaaaa gcagcaattg
     6781 tcatgaatgc gaacccattt acagaagggc attattattt agtaaaacaa gctgccaata
     6841 acaacgattg ggtatatgta tttgtactcg aaaaagaaca aaatcttttt tcgacgaagg
     6901 accgactcaa attagttcaa caaggattac aaaaactgaa aaatgtcatt gttctatcag
     6961 gtggcgagta tattattagc cctgcaacgt ttcccaccta ttttttacga gaagatgatg
     7021 aaatagcaaa ggagcaaatg gtcgtagatg ctactttatt caaagaaaga attgccactt
     7081 atttagatat ctctgctaga tatgtgggag aagaaccact ttccccaatg actcgttcat
     7141 ataacgacat actggaaacg attttacccc ctgaaataaa agttcatgtc cttgcaagga
     7201 agaaaacgga acaacaccaa gttatttctg cttctcaagt tcgtaaagcg tatcttgctg
     7261 ggcaattgga aaaaataaaa tatatggttc cagaaacaac ctatcaatac ttaaaaaata
     7321 aaagggagcg ttaagtaatg gaaataaata aatcagcgat tgctggaaca ttagagtcaa
     7381 gtgatgtcca aatcatgatt tcaccgaata cagaaggaat agaaatcgat ttagacagtc
     7441 aagtgattga gcaatttgga aaacaaatca aaaaagttat ctatgacaca ctagcagttt
     7501 atcagattaa aaacgtaaaa ctcaaggtag tagataaagg ggcattagat tgcgttattg
     7561 ttgcacgatt aaaaacagcg atccatcgtt ccttaggtat ccaacaccaa gaaacaccgt
     7621 gggaggttta attagatgat gaaagaacgt ttgagaagaa caatgatgtt tgttccagga
     7681 aataatccag cgatgatcaa agatgcaggt atttatggtg ctgactcgat tatgttcgac
     7741 ctggaagatg cagtgtctat ggcagaaaaa gacagcgcaa gaatgcttgt ggctgaggca
     7801 ttgaaaacag tggattatgg tgacacagaa ttaattgttc gaataaatga cctgcactca
     7861 gaatttggac gtgcagacat ttttgcaatg gttaaagccg gcgcgcatgt attacgttta
     7921 cctaaaacag aaacctctga agatataaca gaagtagaaa aagtgattat agaagcagaa
     7981 aactatcata atgttccgat agggcgaaca ttgatgatgg cagccattga aggtgcttct
     8041 ggtgtattga acgcacctgc gatcgccaaa gcatctgatc gattgattgg aatcgccatt
     8101 ggggcagaag actatgtgac aaatatgaaa accaaacgat atccagatgg aaacgaattg
     8161 ttttttgctc gcagtatgat cgttcatgct gcacgtgcag caggcatttc cgcatttgac
     8221 actgtttact ctgatgtgga tgatatggaa ggatttttaa acgaagtacg tttaatccat
     8281 caattaggtt ttgatggaaa atcttgtatc aatccacggc aaattccagt agtcaatact
     8341 gtttatcagc cgtcaatcaa agaaatcaaa cgatcactag acatcatctc ggcaatcgaa
     8401 gaagcaaaag aaaaaggttc tggcgtaatt tctttaaatg gaaaaatgat cgataaacca
     8461 attgtagaac gtgcacaacg gacattgatg ttagcaaaag catcacgatt gattgatgag
     8521 gagggaaata tccatgcata ataaacaaag acgtagcatt ccatcaagtg ttttaacaaa
     8581 gtataaaaaa gaattccaaa gtgtttcatt atcgccacaa acgattcaac gttgtgcacc
     8641 aaaagtaacg actgtaacta atcagacaaa taaattgatg cactcgatca aagaagctat
     8701 agaaaaatcg ggcttaaaag atggaatgac gatttcattt catcatcatt tgagagaagg
     8761 tgattttgtt tttaaccaag tgatggccgt gatccgtgaa atgggcatca aagaattgac
     8821 cttagcacca agttctttaa cgaacactat gaatgagatg gttattgaat gcatcaagga
     8881 cggtacagta actcatatca caagcagtgg gatgcgcggc agcttaggtg aatttatttc
     8941 tcatggagga atggacaatc ctgtgatttt acgttctcat ggtaatcgtc cacgtgcaat
     9001 tgaaagtggt gaattgaaaa tcgatgttgc atttctagga gtaccaacat ctgatgatta
     9061 tggaaatgct aatgcgactg ttggaaaatc cgtttttggt tcattagggt atgcaaaagt
     9121 agatgcacaa tatgcagata aagtcattct tctaactgat catttagttc cttaccctaa
     9181 tacgcccatt agtattccgc aaacacaagt cgactatgtc gtggaagtag aagcaattgg
     9241 tgatccgaat aaaatcggtg ctggtgctac acggtttact aaaaacccaa aagaattaaa
     9301 aattgctgaa atggtcaaag atgtcattgt tcactcacct tatttcaaag atggcttttc
     9361 ttttcaaaca ggaacgggtg gtgcagcttt agcagtaacg cgttacttag ctagtgaaat
     9421 ggaagaaaaa cagatcaaag cttcttttgc attaggtggc atcactaaac caacggttga
     9481 tttattggaa aaaggtcttg ttgaaaaagt gttagatgtc caagactttg acaaaggagc
     9541 agctgaagga atggcgcggc ttgagaacca acaggagatc gatgcttcct tttatgctga
     9601 tccaagtaat aaaggagcgg ttgttaacca attagatgtc gctattttat ctgcattaga
     9661 aattgacaca aacttcaatg taaatgttat gactggttca gatggtgtgc taagaggtgc
     9721 gattggtgga catcaagatg cagcaacggc taaacttact atcatctcag cgccgttagt
     9781 acgtggccga atacctacag tagtaaatga tgttactaca ttgatcacac cagggaaaag
     9841 tatcgatgta ttagtcacag aagtaggggt tgctatcaat ccccaacgta aagatctaat
     9901 agctattttt gaaagaattc cacaaattcc tgtttttaca atagaagaat tacagcaaaa
     9961 agcagaaaaa atagtaggca tcccggaacc tttgcaattc acagatcgga tagttgccta
    10021 tgtagaatac cgagatggtt caattttaga tgtcgttcgt caggtgaaag aatttgaaga
    10081 agagagatca tgatgtttga tactggagaa attcaagaaa tcgaggatat tttgatccat
    10141 aaagaacaac gtgctaactt gcaaagcaac ctgctttcca agtattcaaa tagttcattg
    10201 atggtcttta cattgaatat tcctgggccg atcaaaaata atccaatgat tgagcaggta
    10261 tttcgtcagg gacagcagtc aattcagcaa atgtttttaa aaaaaaaggt ggaaatgacg
    10321 tacacaaaag aaatcaattt gccaagtgga ttggactggt acgtaatagt aacagaagca
    10381 cctttgaatc tcaaaaaatg gttggtcacg ttagaagagc aagacgcatt aggtagactt
    10441 tttgatttag atgtcttgtt caaaaaagga gagcagataa caattgtcag tcgccaagct
    10501 cttaatcatg gggaacgtcc ttgttttatt tgtgggaaat cagcaaaggt atgtgcacgt
    10561 aatcgtacac atactgtgaa agaaatgcaa aagaaaatcg aacaaatagt agccgattcc
    10621 ttaaaaggag cattgaaaca tgcatgataa tattcaaaaa atagcacaag aagcacaatt
    10681 agcgatgctt tatgaagtta catgtatcaa taaaccagga ttggttgatc cagtagaccc
    10741 cgggtctcat caagatatgg acatttttac ttttctacaa agtagtgtag ttttgactcc
    10801 ctattttgaa gaatttatcc aaacaggatt ggaccgacaa catcaaccaa ttgaagaaac
    10861 ctttatggcg attcgacaga ttggtttaga ggctgaaact gcgatgttct cagtgacaaa
    10921 tggtataaat acacataaag gtgctatctt ttcattgggg atttttcttg ctatttgtgg
    10981 ccggttaact atttggaaag ttccttgtaa aaaaagtttc tttcaaaaag aaattcaaaa
    11041 aatgacgaaa aatctactta acgattttgg gaagattgat caaacaaaac caaaagcatg
    11101 gacttgggga gaatatctat ttgttaacca tggattgaca gggatacgag gggaagccaa
    11161 aagaggttat ccttgtgttt ttgaccgagg attgccttat tatcaagcat atcaagggtc
    11221 gcaacaagat aagatgatag acactttact ttttctatca atggaagtag aagataccaa
    11281 tttgatcaaa cgaagtaata atagacatgt ttttgaagag tatcaattac ttattcgtcc
    11341 ttattttgaa ttagggggtg taaaaaccat acaaggtaaa aaatatttgg attacctaaa
    11401 tcagcaattt aaaaagaaaa attggagtat tggtggaagt gcagacttat tgatccttac
    11461 aatcttttta gataaaatca tggacaaagg atggttatgt tgatactata atacattatt
    11521 ttgtattagt aagctaataa ctaaaaaatg gttcaataca aatattttat aaaaaatggt
    11581 agtgcctatc ctttcagatt gtagacaaag aaaaatttct ttgtctacaa tttttttgtt
    11641 aatgacttca gtcagtttcg tgcgcaaagc gtgcgaaaca agaaaaccat gcgtttctac
    11701 acgtggaaga actagcatat cgaaaggagt tttttcatgt caaatgacga taaaagttta
    11761 gcccatactc gttggaattt taagtatcat attgtattta caccaaaata tcgacgaaaa
    11821 gtaatctata ggcaattacg aaaagatatt ggaaaaatac ttaggaagtt gtgcaaaata
    11881 aaaaacgtgg aaattataga agcacatgca acgccagatc atattcacat gcttgttcaa
    11941 gtaccatcaa aaataagtat atccagtttt atagttatct gaaagatcgt agtgctgtgc
    12001 taatccacga gcagcacgta aatcacaaat atagatacgg aaataaatca ttttggtcgg
    12061 aggaatatta tgtgagtaca gtcgaattga atcaaaagac aattgaaaaa tacatccgtg
    12121 aacaagaagc tgatgatcgc atttgagata gtataagtaa aagagagtag atgttgcaat
    12181 aacaaaaggg taaaaatgga ctacctccag taaaataccg aaaaatagtt taacttttgg
    12241 agataagtcc atttaagggt ctatagaatg acaatttttt taggggattt tactattatt
    12301 acttcaagac ccgcttggat tcgcaaagtg agtgaactct taaaactacc tgatagtagg
    12361 ttgttttaag agttatctga aagtaaaaat tgcatttata tttttgataa atacgtaaat
    12421 ttatatatac gtatttatca aagttttagg ccaaaggaat attgtgtaaa tacactagga
    12481 ttgaatgaat agaccagaag aaattgcgtc caagaacaaa agaagcatga tagtgctcta
    12541 tataaattgg gcattcaaga atctgaaatt taaaggtaga attttagtta gacttttaga
    12601 ttttaaacac aaaaataacc atacaagcaa caagaagcaa agtgctatat tataaattgt
    12661 ccgtccgttt acttacataa gatcattggg gctattttta aaagagaata atagataata
    12721 aatgaaaaaa aagtcaagtc cacaatcctg tgtaaaatac tatacaatgc tctcactatt
    12781 ttttacttgg caaatttaat atattttctt gatgaagaaa gccactcatc gtgttgatca
    12841 ataaggactg ctccaattaa acgattggct gaaacgcagt ttggaaagat acgaataatt
    12901 ttttctcttc tgcgcacttc ttgattcagt cgttcaatta gattggtact ctttag
//
LOCUS       DACEKG010000082        12946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEKG010000082 DACEKG010000000
VERSION     DACEKG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.085"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(65..1174)
                     /gene="rpoD"
                     /locus_tag="IW664_002266"
     CDS             complement(65..1174)
                     /gene="rpoD"
                     /locus_tag="IW664_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0849610.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1198..3039)
                     /locus_tag="IW664_002267"
     CDS             complement(1198..3039)
                     /locus_tag="IW664_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0849611.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3221..5614)
                     /locus_tag="IW664_002268"
     CDS             complement(3221..5614)
                     /locus_tag="IW664_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0849612.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5721..6581)
                     /locus_tag="IW664_002269"
     CDS             complement(5721..6581)
                     /locus_tag="IW664_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0849613.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6640..7161)
                     /locus_tag="IW664_002270"
     CDS             complement(6640..7161)
                     /locus_tag="IW664_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0849614.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7481..8200
                     /locus_tag="IW664_002271"
     CDS             7481..8200
                     /locus_tag="IW664_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0849615.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8226..10817
                     /gene="mprF"
                     /locus_tag="IW664_002272"
     CDS             8226..10817
                     /gene="mprF"
                     /locus_tag="IW664_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0849616.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11005..12192)
                     /gene="tuf"
                     /locus_tag="IW664_002273"
     CDS             complement(11005..12192)
                     /gene="tuf"
                     /locus_tag="IW664_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0849617.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12560..12916
                     /locus_tag="IW664_002274"
     CDS             12560..12916
                     /locus_tag="IW664_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0849618.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2709 c         2300 g         4224 t
ORIGIN      
        1 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
       61 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      121 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      181 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      241 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      301 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      361 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      421 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      481 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      541 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      601 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      661 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      721 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      781 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      841 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      901 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
      961 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1021 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1081 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1141 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1201 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1261 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1321 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1381 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1441 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1501 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1561 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1621 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1681 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1741 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1801 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1861 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1921 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     1981 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2041 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2101 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2161 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2221 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2281 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2341 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2401 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2461 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2521 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2581 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2641 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2701 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2761 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2821 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2881 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     2941 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3001 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3061 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     3121 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     3181 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     3241 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     3301 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     3361 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     3421 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     3481 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     3541 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     3601 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     3661 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     3721 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     3781 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     3841 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     3901 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     3961 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     4021 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     4081 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     4141 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     4201 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     4261 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     4321 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     4381 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     4441 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     4501 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     4561 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     4621 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     4681 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     4741 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     4801 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     4861 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     4921 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     4981 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     5041 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     5101 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     5161 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     5221 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     5281 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     5341 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     5401 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     5461 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     5521 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     5581 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     5641 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     5701 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     5761 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     5821 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     5881 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     5941 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     6001 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     6061 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     6121 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     6181 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     6241 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     6301 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     6361 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     6421 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     6481 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     6541 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
     6601 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     6661 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
     6721 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
     6781 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
     6841 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
     6901 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
     6961 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
     7021 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
     7081 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
     7141 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
     7201 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
     7261 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
     7321 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
     7381 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
     7441 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
     7501 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
     7561 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
     7621 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
     7681 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
     7741 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
     7801 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
     7861 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
     7921 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
     7981 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
     8041 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
     8101 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
     8161 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
     8221 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
     8281 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
     8341 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
     8401 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
     8461 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
     8521 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
     8581 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
     8641 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
     8701 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
     8761 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
     8821 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
     8881 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
     8941 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
     9001 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
     9061 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
     9121 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
     9181 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
     9241 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
     9301 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
     9361 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
     9421 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
     9481 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
     9541 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
     9601 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
     9661 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
     9721 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
     9781 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
     9841 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
     9901 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
     9961 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    10021 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    10081 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    10141 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    10201 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    10261 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    10321 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    10381 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    10441 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    10501 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    10561 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    10621 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    10681 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    10741 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    10801 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    10861 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    10921 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    10981 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    11041 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    11101 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    11161 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    11221 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    11281 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    11341 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    11401 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    11461 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    11521 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    11581 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    11641 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    11701 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    11761 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    11821 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    11881 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    11941 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    12001 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    12061 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    12121 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    12181 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    12241 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    12301 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    12361 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    12421 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    12481 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    12541 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    12601 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    12661 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    12721 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    12781 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    12841 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    12901 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaa
//
LOCUS       DACEKG010000083        12673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEKG010000083 DACEKG010000000
VERSION     DACEKG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.086"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(147..1178)
                     /gene="queA"
                     /locus_tag="IW664_002275"
     CDS             complement(147..1178)
                     /gene="queA"
                     /locus_tag="IW664_002275"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0849619.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1371..2546
                     /locus_tag="IW664_002276"
     CDS             1371..2546
                     /locus_tag="IW664_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0849620.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2550..3191
                     /locus_tag="IW664_002277"
     CDS             2550..3191
                     /locus_tag="IW664_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0849621.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3188..4105
                     /locus_tag="IW664_002278"
     CDS             3188..4105
                     /locus_tag="IW664_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0849622.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4102..4773
                     /locus_tag="IW664_002279"
     CDS             4102..4773
                     /locus_tag="IW664_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0849623.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4969..5803)
                     /locus_tag="IW664_002280"
                     /pseudo
     CDS             complement(4969..5803)
                     /locus_tag="IW664_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6004..6270)
                     /locus_tag="IW664_002281"
     CDS             complement(6004..6270)
                     /locus_tag="IW664_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0849624.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6270..6533)
                     /locus_tag="IW664_002282"
     CDS             complement(6270..6533)
                     /locus_tag="IW664_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0849625.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            7008..7193
                     /locus_tag="IW664_002283"
     CDS             7008..7193
                     /locus_tag="IW664_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0849626.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7335..9158)
                     /locus_tag="IW664_002284"
     CDS             complement(7335..9158)
                     /locus_tag="IW664_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0849627.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9755..12460)
                     /locus_tag="IW664_002285"
     CDS             complement(9755..12460)
                     /locus_tag="IW664_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0849628.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3651 a         2637 c         2389 g         3996 t
ORIGIN      
        1 ttcaaggtgt cttttctata tgttttggaa aataaaaaag atgctggtgt catccaccaa
       61 catcaaaaag tatagagctc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
      121 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
      181 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
      241 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
      301 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
      361 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
      421 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc tgcttcttcc
      481 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
      541 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
      601 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
      661 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
      721 ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc ttcatactca
      781 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctaccgcttg caatctgcca
      841 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggttttat caatgtctcc
      901 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
      961 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
     1021 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc aattttccga
     1081 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gatcaactct
     1141 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
     1201 gtgcttcatc attctatcat gtttttcatt ctgttggcaa aatcagcact ttttcacttc
     1261 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
     1321 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
     1381 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
     1441 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
     1501 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
     1561 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
     1621 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cacccttgtc caaaaattgt
     1681 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
     1741 tgccccgtga gatgttagat cgctacccac atgaattatc tggtgggcag cagcagcgta
     1801 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
     1861 gtgcgcttga tcccatcact cgtgattctt tgcaagacct tgtcaaagat ttacaagagc
     1921 gcttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aagctagcaa
     1981 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatatct
     2041 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
     2101 ccaagccaaa tgcgacaaga gtcggagaag ttatgctaaa tacagcaatc acgattacac
     2161 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
     2221 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
     2281 agcgaggaaa agtatccagt gtcggcgaca tattaaataa acatgttttt tacgtaaaag
     2341 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcggactg aaatacgtgc
     2401 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
     2461 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagata
     2521 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
     2581 aaattctctc aaaaggtttt gaacatcttt atatttcggg acttgctttg atcattggta
     2641 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
     2701 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
     2761 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
     2821 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     2881 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     2941 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     3001 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     3061 attaccaacc agatctcatt ttggcaggta cgatccctgt aacgatttta gcactattgg
     3121 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     3181 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     3241 gagttgttct ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     3301 tggaatcact tcagaagcac agatcttagc aagcctagtt gccggaatgg ttgaacatta
     3361 tacagataaa aataccgcaa tcatcaacaa tctggctacc acaactatca accatcaagc
     3421 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     3481 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     3541 atttgagaaa agatacgatc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt
     3601 ctttcttgtt cgaaaagaca ccgcacaaaa ataccattta tctaaagtca gcgatctgaa
     3661 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     3721 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     3781 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     3841 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     3901 cccaccatat gatgctgctc cagttgtaaa caacaaaata ttagctgata cgcctcattt
     3961 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     4021 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
     4081 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
     4141 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
     4201 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
     4261 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
     4321 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
     4381 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
     4441 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
     4501 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
     4561 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
     4621 ggagatatca ttatccgagg aaccaatgca acagatggca catcgattat tttagcaggt
     4681 gcattgccaa ctgcattaat ggtgattgtc acggactggc ttttaggtat tttggaaaga
     4741 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
     4801 cgaccatttg ttggtcgcaa aggctttttt agttctgtca ttttttaagg caataatcca
     4861 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
     4921 tatactccaa cttcatactg ccaacgggtt catctctgca tccaccgact aagttttttt
     4981 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
     5041 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
     5101 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
     5161 acatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
     5221 aacttgccat cctcatcctt ggctaaaaat atatctagcc attccagctt ctcgccatgg
     5281 gaggtttgcg gttcctaaaa ccggtacatg attcttatgg actgcatccg tcgcaactag
     5341 acttggcggt acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
     5401 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
     5461 tgatactgct attcatggcc gaaagcgcca cgactttgac ttcttccgtt tgagtgacat
     5521 tcatgttcgg caatgtcgct ttggataccc gctctgctaa agaaacgaca ctggtattgt
     5581 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
     5641 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
     5701 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
     5761 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
     5821 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
     5881 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
     5941 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
     6001 acttcactca taatgtgtcc aggcagaata aatttccaca actttatttt cctcatcaac
     6061 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
     6121 tctcagcttc tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
     6181 ttcttcaaat tgtttcttta aattcgattg cttgattttt cttaaatcca ctgtcgcaga
     6241 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
     6301 ttcgtaaagc cactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
     6361 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcta gcccaatcaa tacagcattg
     6421 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
     6481 aactgataaa aatttttacg agctgtgcta ggattcatcg ttgtattagc cattgtctct
     6541 cctccttttt ctgatataat tataacgtac ttgattaagt acgtcaaaaa atacaataaa
     6601 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
     6661 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
     6721 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
     6781 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
     6841 acctgaggat acccacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
     6901 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gccattcatg ttcactatcg
     6961 atttgatact tgcttagacc gtcgggtttg tgaatcacca attaaaaatg gcactgaatt
     7021 atggtaacaa aaaatggtca cttgacgagg ctgagatttc tacttatgaa gacacatggc
     7081 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
     7141 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
     7201 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
     7261 tgggaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
     7321 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
     7381 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
     7441 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
     7501 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
     7561 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
     7621 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
     7681 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
     7741 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
     7801 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
     7861 aaaggccaaa tgtcccctaa ccgatagacg aacaaaatcg caatgattgc atatgagatg
     7921 aacgcacctg taatattggc aatcgacttg cttaaccatc tttttccttc ttttttccat
     7981 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
     8041 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
     8101 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
     8161 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
     8221 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
     8281 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
     8341 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
     8401 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
     8461 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
     8521 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
     8581 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
     8641 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
     8701 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
     8761 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
     8821 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
     8881 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
     8941 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
     9001 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
     9061 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
     9121 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
     9181 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
     9241 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
     9301 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
     9361 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
     9421 atcttaactt ttgaaagaga gatgtaaggt tgctttctac ccatcattct tactactcga
     9481 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
     9541 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
     9601 tgatgcagaa cagccttttc acaacctctt ggtacacggt gttcaatcag ctgaccctcg
     9661 gcattagctc aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
     9721 tcaggtcaaa acgctgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
     9781 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
     9841 ttccaaagac aacaaacaga ccgatcatga tccagattgc tttcgtagca tttggccagt
     9901 aaatgcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
     9961 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    10021 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    10081 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    10141 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    10201 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    10261 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    10321 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    10381 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    10441 ttgttgcatc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    10501 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    10561 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    10621 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    10681 ccaatcctaa tttttgctga aggatcggta attcattgtt gtataggtct gccccttcat
    10741 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    10801 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    10861 cagttgcttt cgttgaactg agaagcgaat caaagtcgat ctggtccgct ttgcttagcg
    10921 ccccttgcgc agtcccaatc gtatcgatga cttggtctaa tagagaagtg acagtggaac
    10981 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    11041 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    11101 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    11161 ttggctgcaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    11221 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    11281 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    11341 ttaacgaacc tgtcgttgtt gccacttgtc caattgattt caatgtaaca tctacactgt
    11401 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    11461 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcctgt acttgttgga
    11521 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    11581 caaaatcgcc gtcgatctct gctagttgtt taccggcatt ttggatctct gggatttttt
    11641 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    11701 caacgacttt ttcacccatc tcatctactt tagggatata atccacgaat tcatttgctt
    11761 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    11821 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatctttg actttattga
    11881 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    11941 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    12001 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    12061 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    12121 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    12181 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    12241 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    12301 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    12361 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    12421 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    12481 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    12541 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    12601 ataattaagg attacagact ttactttatc atgaaataaa agggaaagat aattttatgc
    12661 caaagttttt atc
//
LOCUS       DACEKG010000084        12113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEKG010000084 DACEKG010000000
VERSION     DACEKG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.087"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            126..1151
                     /locus_tag="IW664_002286"
     CDS             126..1151
                     /locus_tag="IW664_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0849629.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            1190..2140
                     /gene="manA"
                     /locus_tag="IW664_002287"
     CDS             1190..2140
                     /gene="manA"
                     /locus_tag="IW664_002287"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0849630.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            2234..3937
                     /locus_tag="IW664_002288"
     CDS             2234..3937
                     /locus_tag="IW664_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0849631.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            3939..5972
                     /locus_tag="IW664_002289"
     CDS             3939..5972
                     /locus_tag="IW664_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0849632.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            5983..6423
                     /locus_tag="IW664_002290"
     CDS             5983..6423
                     /locus_tag="IW664_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0849633.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6623..7750)
                     /locus_tag="IW664_002291"
     CDS             complement(6623..7750)
                     /locus_tag="IW664_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0849634.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(7753..8430)
                     /locus_tag="IW664_002292"
     CDS             complement(7753..8430)
                     /locus_tag="IW664_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849635.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            8694..10460
                     /locus_tag="IW664_002293"
     CDS             8694..10460
                     /locus_tag="IW664_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0849636.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            10475..10912
                     /locus_tag="IW664_002294"
     CDS             10475..10912
                     /locus_tag="IW664_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0849637.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            10925..12076
                     /locus_tag="IW664_002295"
     CDS             10925..12076
                     /locus_tag="IW664_002295"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0849638.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4088 a         2130 c         2504 g         3391 t
ORIGIN      
        1 aatagtatac cgggggaaac tgcgcggttt ttcgcgcagt ttttttgtgt aaaaaaggtt
       61 gattcctcat agattaagct ttaagcttgg actgtaagaa tctgatagaa aagcaggaga
      121 aaaagatgta tccacacacg aaacagctta ttttgaaaaa gatggaagaa ggattttatc
      181 caggtgtagc agtcgccttc ataaaagaaa atcaagtaga aacatttaca gccggctttg
      241 cccaagtcgt tcctgaaaag cagctgatga cagaagatct gttgtttgat gcagcctcac
      301 tgacgaaagt cgttgcaacc acttcattag ttttacgtct attagaagat ggaaagatag
      361 aactcgatca accgcttcat aaatatcttc ctgaatttga aaatcagaca atcactttgc
      421 gtcatttact gacacatact tctgatatca aaacatggat accaaacaga gataagctat
      481 ctcaggaaga attgatcgcc gcttatttga agctagaacc aggtgagcaa ctaggaaaag
      541 aagtaaaata tacagatgcc ggtacgattc ttcttgggtt gatgctagag gaaatttacc
      601 aagaaactgc agtggaaatc tttcaagaac aagtattgga accattagga atgatgaaca
      661 gcttatttct tccttttcct tccaaaaaaa tcgttcccac agaacaactg ccttcgggtg
      721 aggttttgcg tggacaaact catgatccga aagcaagagt tttggctgaa catgcaggta
      781 acgcgggtct ctttattgat ttgaaagaca gcattacttt tgtccaaatg tatttgaaca
      841 agggaaaaat ccgtaatggt cgttttttag aagaagagac gattgattct ttactgcaag
      901 atcaaacacc gaaaaaaaat ggctatcgtt ctttaggatg ggacttaaaa aaggatgtag
      961 aagacggtca tgcgattctt tttcatactg gttatacagg tacgtttttc ttgattgata
     1021 taaaaacaca agaagcgttg atatttttgt caaatcgcgt acatcctgtc gatcatcgtg
     1081 cggagtatct catatgtcga gatgaaattg ttgaaaactt tttgaaagag aaagcggtat
     1141 taccgaaata atgctataat agctatgaaa aatgagagga gtttgaatta tgcaggaacc
     1201 tatgttttta aaaccagttt ttcaagaaaa aatttgggga ggaagtcgtt tacgttcagt
     1261 ctttggtttt gatatcccta acgataaaat cggagaagat tgggcgatca gtgcgcatcc
     1321 tcatggtgtc agtgtagtag aaaatggcga attcaaaggg aaaagattag atgaactatg
     1381 gtcagaacac aaggaattgt ttggccatcc ttcagaacca gtgtttccat tattgatcaa
     1441 gatcttagac gcagaagatg atctatctgt acaagtacat ccagatgatg cctatgggat
     1501 gaaacatgaa ggtgaattag gaaaaactga atgctggtat atcatcgatg ctgaacctgg
     1561 tgcagaaatc atctatggtc atcatgcaaa gacaagagaa gaactagctg agatgatcaa
     1621 agatgggcgc tgggatgacc ttttgaagaa agtaccagtt aaaaaaggtg atttctttta
     1681 tgtaccaagc ggaacgatcc atgctattgg taaaggaatc atgattttag aaacacagca
     1741 aagttctgac acgacttacc gcgtgtatga ctacgacaga aaagatgcgc aaggacaaac
     1801 aagagagctg catatccaac aatcgatcga tgtaacaact gtccctgcga aaacaccaga
     1861 attacaaatc aaagaagtcc gaaaagggaa ttcatcgatc gtcacttatt tagaaactga
     1921 gtttttcaat gtatatgagt gggatattaa aggaatcaca tctttcaaaa aacaagcccc
     1981 ttatacattg atgacggtaa tcgaaggagc aggagacctt gtcgttgacg gaaaaacata
     2041 tccattagaa aaaggaacaa gctgtattat tccaaatggc gtttctgagt gggcggtcca
     2101 aggtgaacta gcaatcattg cgactgaacc aggcaaatag ttcccagata gctgaaagca
     2161 gcagacggtt tttcgtttgc tgctttttct acataaaatc cgtccatcaa taaatagaag
     2221 tatggtgaaa aagatggaat acaaatggaa agcaaacaac agcagtcgcg tgtctatgaa
     2281 acaactgaga aaagctggac atcacttaaa tgaaatgatc atgccaaatc taagtctgtt
     2341 tatcgcttgg ggaatcttgt cattagcaac aaaaggtgtg tctggcgatt tgcacaccac
     2401 attagaagag gtatgccatt ggatgatcca gctgctttta cccatcttga tcagttattc
     2461 aggcgggaaa tcggtagggg gacaaaaagg ggcagtcgcc ggtgcaattg cttctttagg
     2521 gttgatcgct cgttccaatg ctccacaaat cgtaggagcg atgattatcg ggccgcttgc
     2581 cggcatcgta tatgaacaat ttgtttctag attccacgtg aaattcaagg ctggatatga
     2641 aatgcttgtc agcaacctac tagtaggact gactggtagc gttatttgta taattggaat
     2701 cgtttttatt gaaccagttt taaatagtgt tcacttgttg ctcgcctccg ttgtgtcttg
     2761 gctagtcaca atgaatctgt taccacttgt cagtctgatc ttagaacctc taaaagtact
     2821 atttttcaat aatgcaatca atcatggtgt attgacacct ttaggaatcg aattctcaca
     2881 aacagtcggg tactcttcct tatttttgat ggaggcaaat cctggacctg gtttagggat
     2941 cttactttct atcctttgtt ttgcagaaaa acaggaaaaa gtaaatgcga gtggagcatt
     3001 gatgatccat gcgattggcg ggattcacga aatctatttt ccatttgtct tgctgcgacc
     3061 gtatctattt ttagctgtaa tggctggtgg ggcaagcggc acgttgatat ttcaatggtt
     3121 agatacggga ttagctgcac ctgtatcgcc tgggtcacta ctcgtgattt ttgccaatgc
     3181 gccttatcca caactagctg gggtaagtat agggatagca atcagtacat ttgtgacctt
     3241 catttgtgca gcgatactat tgaaattacc aaataaagaa aaagagaaca aaacggtcaa
     3301 agaaagggga gaaatcttgg aaccatctat cattcattcc gtcatttttg cttgcgactc
     3361 aggtatggga tcaagtgcta tgggagccgc cctgctgaaa aagaaattag cagcagaaaa
     3421 aatcgatctt tctgtcggat acagctctat ctatgaattg aataaccaag taaatcagct
     3481 ggttatcgtg caacatgaac tagcgggtct tgcaagagaa aaagtacctc aaagtcaaat
     3541 ctatgtactg gatcactttt tggaaacaga tatcttgctg tcacgaataa aagaagcgat
     3601 ccaatttcca aagcgggaag aaaaaaaaga gatagcagat atcacagaac aaaaaaagtc
     3661 gcctgctaat attgtatttt tgtataaaaa caacagacgc ggctcacaaa ctatgggcat
     3721 gacgatttta agtcaattag caaaaaaagc gaaaaaacag tttgttatct caaaagaatc
     3781 aatagaaaaa atcaaaatcc aagaagaaac gatttatatc gcacaaagag aactagctgt
     3841 aactgaacag ctagaagaaa agatccctcg gctagttcta gttgaacatt gggtagctac
     3901 gaatgaatac gaaaaactcg tgaagggaga aatataagat gtatttatcg cccagagaaa
     3961 aacaattatt agcagaattt ctcagtagcc cgtcacctgt atctatccaa aaaatgatga
     4021 atttattgaa ggtcagtaaa cggactattt accgcgaatt agataatctt gaagtctcat
     4081 tgaaatcggt aaatgcttct ttgaaaaaag cagctagagg ctcatttgtc attgaggcat
     4141 cagaagaagt attagagcaa ttaaaagaaa atacagatgg gaaaagtaat gacttatcca
     4201 ctgctaaacg acaacacgcg attttagccg agttgttact gacgacagag ccgttgagtc
     4261 ttgctcattt catggaatca tatcgcatca gtaataccac tttttatgca gatatcaaac
     4321 agttggaaga aagtattcgc caactgcctt tggaaatcat tcggaaccag ggatacgaga
     4381 tcgaaggacc ggaaaaatac cgccggcttc tgacagcgaa tgtattagaa ttagaaatca
     4441 atgaatatga actttttcat agtatttctt tcgatgcatc actgaattat ttttttcgat
     4501 tcgttgatcc gcaacatcta tctcttgcac gcaaagtaat cggggaagaa ttgatccaaa
     4561 aaaagaccaa tctaagtgat agaaagcttg aacatttggt tttgatgctg acattgacga
     4621 tggagcgtgt gactaaaaat catttactga caaatgagac atataatagt ctggcaaaca
     4681 aagaattact acagacagct aaacgattgt tttcacggat cgcttctgag acaaaacaac
     4741 tttatccagt aaatgagatc gtcttttttg caggattatt aaatgacttc acgaactcat
     4801 ttgatcagaa tttctttgaa gagacgttcg atacacagct tgcttattta gtaaaacaac
     4861 tgattcaaga agtaagtgaa gagacgaatg tccattttta tgaagatgaa actttgtaca
     4921 aaatgctgtt gactcattta tctggtgtgt tttcaagagc cgttttacaa gaagagacac
     4981 tgaccaatcc gattttagaa aaaatcatgg ttcaatacga ggaagttgcg aatgcaatcc
     5041 gtaaagcagt agagcggatt tttcaagaaa aaaaattatc cgaagaagag atcgcttata
     5101 tggtccttca ttttgcgaat tcattggaac gaagtccgaa agaaagcaat atcagtattg
     5161 caggattttc gccaagtgga ttagcttcta ctagcatgtt ggaactacga ttgaaaaggt
     5221 attttccctt catccaacaa attcatttct atcggattgc tgatttaaaa aaaatcgatt
     5281 tacaagaaga atatgatgtt gttatttcta catctatttt accaggatac ggtggaaaat
     5341 atattttagt ctccccgcta ctgctagaag atgaagtcaa gcagctaaaa gaagcttttt
     5401 cgcatattga gaagaaaaaa aggcaaaaaa ttacgtataa cagcaataac gagtggatga
     5461 tagaagatac ttatgaggaa acaatgcatt tcatggatca aatcaatcaa ttactcggtc
     5521 gtttttttat ccaagaactg gataatcctg agacgatttc cgaaacgatt cgcagcttat
     5581 ttccttattt tcctgaagga ctagtaacgg atgaagaggt ggttcatcgc cggctgctta
     5641 agcgatatga acaagcacct atcggtattc ctcatacaaa tatggggtta ttccatacat
     5701 ccagctcatt gatttctaaa ccgatctttt gtattttcaa tttgaaacat ccgttgacca
     5761 ttgaagggat ggatagacag ccagtcgatt tgtcccgtat gttagttatg ctggcgcctt
     5821 cgccaatcca agaaaaagac gggattcttt taggaaagat cagtggagca atcatcatga
     5881 atgacttgaa caccgagatc ttccgctcag gaaataaaga aatcgtttat cagttgttag
     5941 cgaaaatcct gattgaagaa ataaagaaat gaaggtgaaa agatgataca gctaccagca
     6001 gaaaaaatat tgctcgatca atcctatcaa aattggaaag aagcgttgac cgagatcggc
     6061 cagtggatga cccaacaagg gggaatcaaa ccggcatata tccaatcaat gatcgaaaga
     6121 caagaaaaag cgagtgtcta tatcgggaat ttcgtagcct tgccacatgg gactgacaaa
     6181 gatgaagaag tagtagaaga aggaatctat ttagtccaag tgcctgatgg tgtggacttt
     6241 ggaacaaaag aggaaccaaa aatcgcgaca ctcttatttg tagtggcttt aaaaaaagaa
     6301 catcagttag cggtgctcca agatttagcc tttttctgtt cagacatcga ccaagtaatg
     6361 gcgctcagtg atgcgaaaac cattgaagaa gtccaaatga tttttgaaca tggacatata
     6421 taaccaatgt tcacagtaaa aaaagctgcc ttgcatcaag taataacttc aaccaagata
     6481 aaaataagag gagctatttt tgttgggagg ttcttattac tgcggatgca agcttttgaa
     6541 aactatttat tctagaaaac cgtttatttg aaatagggga cggtgacaag gtttttgtca
     6601 ccgcccctat tatttttgtg ttttaatagc tcttttttat atagatagtg cccataccta
     6661 tgaataataa aacgatactc catataccaa gtgtcaagat accattcatg aaagtaacct
     6721 gttctgaagc tagtggcgag aaaatatctc ctcctatgat cacattttga aaatcaacat
     6781 aagaagttgg caagtaatga ctgattgatt ctatccatcc aatcgaagaa aacaagcgat
     6841 actctgctaa gaaaagacaa agaagtgtac ctaaaagatt gactagagaa tgttcaaata
     6901 aagcagatag aagaaaactg attgtactta aaaagagcac ccacaacaac aggaacagaa
     6961 agctctggaa aagatactca tacatcggaa ggattcgtat ctcctgacca atgatcgttc
     7021 ctacaggata acggaagtct cccaaaccat ttttgattat tactagtata gagacgatca
     7081 aaaatggcat cacgagactt agcatagctg caccataata aatggtaaaa cggatgctat
     7141 gtttctttac catactatcc ggtaatagat tgactagaga gattacctgc tttctttttt
     7201 cataagagat gatggaagaa agaaacaagg cggttataca taaaatcaaa aaactagaaa
     7261 tcattcctga aaaaatattt tcgtagtaat ttgctaaagg gagatgcgct ggcaagtcaa
     7321 aaatagaata tctttggatc tgatgttctt tcatataacg gagcaactcg acacgttttt
     7381 gttgttcaat gattggaatt ccttgtcttt gaccagaaat cagtcgatcc agtatatctt
     7441 tttcataatg ataagttgct tcgaccattt gctggacatt ccctgtgttt tttgcatgta
     7501 agattgcttc gatcagtttg ttgcttttct ctaaactttc cacaaattct ttctcattct
     7561 catcttcttt caacgtttca atcaaacgtt gcatctcttc atagttatga ttcagttcat
     7621 cgaattctgt ataagcaagt ttatgatcaa aaaaaagtgg gaatgcaaag agcacgacca
     7681 acaatagagg aaataaaaag tttactttgt tttttagtaa gagattgcgt tcaaatgtaa
     7741 gtaaagacat agttacacct catataattt acgataaata gctaaaatct cttccatatt
     7801 tgaggattct gtgacgattt ctccattttt tagaaaacat gctttagaac aaatcgattg
     7861 gatattcgcg atagcgtggg aagaaagaag aatcgtcttc ccctctttct gccattgtct
     7921 gatgaggcta ttcaagatat caatacttcc tgggtctaat ccgttcaacg gttcatcgaa
     7981 taacatgaca gatgaatcac tgatcaagta agcagcaagc aagacatgtt gtttcattcc
     8041 tagagacatt tttccaatcg gcaaagtaag gtactcagtc atcttcaatc gttggaaaac
     8101 agtttggcat tctactttgc tctgccaata gtatttgatc aaagtcaaat aatcaacagc
     8161 cgatagatag tcggataact gattcgttga ttctagaaaa aagaaagacc ggcgataatc
     8221 tgtataagca gtaggagaga tgttgtgaag ggacaattga tcataagttg ttggcagcag
     8281 ttgggcaatg ccattcagca aagttgtttt gccaaatccg ttcggggcaa tcaaagcagc
     8341 tacttctcct gattctacta aaagattcaa ttgtcgaatc acacgttttt taccacgttt
     8401 catgttcatg tttttgatca gtaatgtcat agataggcct ccaatataaa atttaataac
     8461 ataatagaat ggaaaacatc taaattaaat gctaacaaaa gttattgttt gcatattata
     8521 aaaaaattaa aataacagtt ttatttaaaa ttgaagtttt ctgataccga aatcttttta
     8581 ttttgctgac atactcaaac ctttggcaca aagtttcgtg ccaaagtacg actatttctc
     8641 tttttagtaa gcggatacaa ttagagtatc aaaaggagag agggagagac aaaatggaac
     8701 ggacgattcc caaaaacaac atttcgctaa aagcgagggt acaaaaatta ggcagtactt
     8761 tatcaagcat ggtcatgcca aatattggag cactgattgc ctggggtgta ttaacggctc
     8821 tatttatccc agatggttat ttaccaaatg aggctttggc taccatggta agcccaatgc
     8881 taacttatct gattccttta ttgatcggtt atactggcgg aaaagtaatt gctggtgacc
     8941 gcggatcagt tgttggggca attgcaacga tgggtgtgat tgtcggaaca gatattccaa
     9001 tgatgctagg cgcgatgatc atgggacctc ttggtggata tgccatcaag aaatttgatc
     9061 agctattcca aaaacggatc aaatccggtt ttgaaatgct agtcaataat ttttcagcag
     9121 gtcttatcgg ttttggatta gcgttattag gtttttctgc gattggtcca gttgtcgatg
     9181 cattgactca agcaatggca aaaggtgtag agatcatttt atctgcccat ttgattccat
     9241 tgacaagtat ttttattgaa ccagcaaaaa tcttattttt gaataatgca atcaatcatg
     9301 ggattttgac accattggga actgaacaag tagcagaggc tggaaaatct attctattct
     9361 tgcttgaagc aaatcctggt ccaggacttg gcgtattact tgcttttact ttgtttggca
     9421 aaggagcagc aaaatcttca gcaccaggag ccatgatcat tcacttttta ggcgggatcc
     9481 atgaaatcta ttttccttac gttatgatga aaccgttatt attcttatca gttataggcg
     9541 gaggaatgtc aggaagtttc attttccaat tgatggatgc tggactaaga gcgccagctt
     9601 ctccagggtc gatcattgcg attctagcga tgacaccgtt caattcttac cttcctgtta
     9661 ttttgggcgt gattgttgcc gcagctgttt cgtttggtat ttctgcagta attttgaaag
     9721 cggatgcgaa agatacaaat gaagacttcg aaaaatctgt tcaagagaca aaggcagcta
     9781 agcaagcttc caaaggtgcc caaggtattc ctcaaggagc tacattctct ggcgtacaaa
     9841 aaatcatttt tgcttgtgat gcaggaatgg gatcaagtgc gatgggcgct tcgatcttga
     9901 gaaaaaaagt acaggaagca gacttgcctc aaacggtgac caatcaagca atcagccaac
     9961 tgacagatga ctcgaataca ttgatcgtta ctcaggtcga actacaagaa agagcaaaac
    10021 aaaaagcacc gagcgcgcgt tttgtctcag tagaaaactt tttgaattca cctcgatatg
    10081 atgaaatcat tcaagaatta agcagcaagc agaaaacatc agtttctgaa attgagaatg
    10141 agccagcaga agagtttctt tcagcaaaca tcaaacaaat caaagagatt ctacttgtcc
    10201 acgatgatcg ttccggatca gcaacaatgg ggatgaccgt gctcaaagat atattagaca
    10261 agaaccattt aactatcccg ttaagaaaag tcaatatcca agatttaaaa gatgaacctg
    10321 attcactgat cgtagccaaa gaaagtttag cggaaaaagt acagatggct gcacctcaat
    10381 ccattcattt atcagtcaac agcatggtca atccgcaaaa atacgaatcg attgcacaat
    10441 atttgaaaca gacagcttaa gggaggaaaa cagcatgcaa agtttgccat tcgacatgat
    10501 cgagctcaat cgatctttta aaaccaaaga agaagcgatc cgctattgcg gtcagatgct
    10561 tgtagaagca gggtgcgttg atgaggaata tatcgatgcc atggtagata gagacaaaat
    10621 gttatctgtt tatatgggga attttatcgc gattcctcat ggaacagatg aagcaaaaaa
    10681 atatgtgaaa aaatccggca tctgtgtcgt tcaagtacca gatggtgtag attttggtac
    10741 cgaagaagaa gaaaagattg cgactgtttt gttcggtatt gcaggagtcg gagatgagca
    10801 tttgcaactt gtacagcaaa tcgcacttta ttgcagtgat atggacaatg ttgtccagct
    10861 agcagacgcg ctcagcaaag aagaagtaac ggaaaatcta gcaattgctt aaggagagag
    10921 aaaaatgaga gcagtacatt ttggtgcagg aaatatcggt cgaggattta ttggagaggt
    10981 tttggcagct aatggatttg agattacttt cgtagatgtg aatcacacga tcattgatgc
    11041 tttaaatgaa agaaaagaat acgaaatcga attagcagat gaatcaaaag aaaaaatcac
    11101 tgtaaaaaat gtcaaaggaa tcaacaatca aacaaatcca gaaaaagtaa tccaagaaat
    11161 cgctgaagca gatttagtta ctaccgccat tggcccgaat atcttacctt ttattgctga
    11221 gttgattgca aaaggtatcc aagaaagaga aaaggaagga agcacaacat ctttagatat
    11281 cattgcctgt gaaaatatga ttggcggttc agcatttcta gaaaaagaag tgtataaata
    11341 tcttccggaa acatcattta ctgacaaata tatcggcttt ccaaatgcag cagtcgatcg
    11401 aatcgtccca ttacaacacc atgaagatcc tttattcgtc caagtggaac ctttcaaaga
    11461 atgggtcatt gacgatagcc aaagaaaaaa taaaaaaatc caattaaaag gggttttata
    11521 tgtagatgat ctagaaccat atatcgaacg caaactattt agtgtcaata ctggtcatgc
    11581 gactgtcgct tatacaggtg ccttgttagg ttaccagacc attgatgaag caatgcaaga
    11641 tgcgttagtt gtggcacaat taaaatcagt tttgcaggaa acaggaagtt tgctgattgc
    11701 caaatggggg tttgatgcag agcagcatca tgcgtatatt gaaaaaatca ttcatcgctt
    11761 ccaaaacaaa aatatctctg atgcgatcac acgtgtcgct cggacaccat tacgtaagtt
    11821 aggttatcaa gaaagattta cacgtccagt gagagaacta caagaacata acttgacttg
    11881 cccacatttg acggcaacaa tggggattat ttttaattac tatgatcctg aagatgaaca
    11941 aagccgtcaa ttgcatgaaa tgaagatcca tgaaaacttg gaacaattga ttcaagaagt
    12001 cacaggaatc aatgatccga aaacaatcgg gaatatcaag caaaacgtga accgttatgc
    12061 caagcaagtc gcttaaaata ggatacgaaa aaggggaatg tgacgaaatt ttt
//
LOCUS       DACEKG010000085        11351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEKG010000085 DACEKG010000000
VERSION     DACEKG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.088"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..305
                     /locus_tag="IW664_002296"
     CDS             6..305
                     /locus_tag="IW664_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0849639.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IW664_002297"
     CDS             378..1739
                     /locus_tag="IW664_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0849640.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IW664_002298"
     CDS             1761..2021
                     /locus_tag="IW664_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849641.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IW664_002299"
     CDS             2035..2787
                     /locus_tag="IW664_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0849642.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IW664_002300"
     CDS             complement(2881..3486)
                     /locus_tag="IW664_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0849643.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IW664_002301"
     CDS             complement(3539..4513)
                     /locus_tag="IW664_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0849644.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IW664_002302"
     CDS             complement(4891..5670)
                     /locus_tag="IW664_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0849645.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSRRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW664_002303"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW664_002303"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0849646.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IW664_002304"
     CDS             7161..8558
                     /locus_tag="IW664_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0849647.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IW664_002305"
     CDS             8639..9079
                     /locus_tag="IW664_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0849648.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IW664_002306"
     CDS             9227..9553
                     /locus_tag="IW664_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0849649.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IW664_002307"
     CDS             9554..10303
                     /locus_tag="IW664_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849650.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IW664_002308"
     CDS             complement(10383..10838)
                     /locus_tag="IW664_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0849651.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3600 a         1975 c         2317 g         3459 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgccgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt t
//
LOCUS       DACEKG010000086        11253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEKG010000086 DACEKG010000000
VERSION     DACEKG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.089"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(40..1419)
                     /locus_tag="IW664_002309"
     CDS             complement(40..1419)
                     /locus_tag="IW664_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ0849652.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="IW664_002310"
     CDS             complement(1440..2987)
                     /locus_tag="IW664_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0849653.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="IW664_002311"
     CDS             complement(3236..4015)
                     /locus_tag="IW664_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0849654.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="IW664_002312"
     CDS             complement(4090..4548)
                     /locus_tag="IW664_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0849655.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5819)
                     /locus_tag="IW664_002313"
     CDS             complement(4560..5819)
                     /locus_tag="IW664_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ0849656.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="IW664_002314"
     CDS             complement(5935..6249)
                     /locus_tag="IW664_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0849657.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="IW664_002315"
     CDS             complement(6395..7891)
                     /locus_tag="IW664_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ0849658.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="IW664_002316"
     CDS             complement(8069..8548)
                     /locus_tag="IW664_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ0849659.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8924..9169)
                     /locus_tag="IW664_002317"
     CDS             complement(8924..9169)
                     /locus_tag="IW664_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0849660.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="IW664_002318"
     CDS             9274..10047
                     /locus_tag="IW664_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849661.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="IW664_002319"
     CDS             complement(10276..10524)
                     /locus_tag="IW664_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0849662.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="IW664_002320"
     CDS             complement(10623..10937)
                     /locus_tag="IW664_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849663.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3500 a         2317 c         1684 g         3752 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt att
//
LOCUS       DACEKG010000087        11089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEKG010000087 DACEKG010000000
VERSION     DACEKG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.090"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..407
                     /locus_tag="IW664_002321"
                     /pseudo
     CDS             1..407
                     /locus_tag="IW664_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(309..1025)
                     /locus_tag="IW664_002322"
     CDS             complement(309..1025)
                     /locus_tag="IW664_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0849664.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1149..1334)
                     /locus_tag="IW664_002323"
     CDS             complement(1149..1334)
                     /locus_tag="IW664_002323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849665.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1561..2834
                     /locus_tag="IW664_002324"
                     /pseudo
     CDS             1561..2834
                     /locus_tag="IW664_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2941..3972)
                     /locus_tag="IW664_002325"
     CDS             complement(2941..3972)
                     /locus_tag="IW664_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849666.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3979..4809)
                     /locus_tag="IW664_002326"
     CDS             complement(3979..4809)
                     /locus_tag="IW664_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0849667.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4806..5612)
                     /locus_tag="IW664_002327"
     CDS             complement(4806..5612)
                     /locus_tag="IW664_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0849668.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5618..6442)
                     /locus_tag="IW664_002328"
     CDS             complement(5618..6442)
                     /locus_tag="IW664_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0849669.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6432..7532)
                     /locus_tag="IW664_002329"
     CDS             complement(6432..7532)
                     /locus_tag="IW664_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0849670.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7710..8495
                     /locus_tag="IW664_002330"
     CDS             7710..8495
                     /locus_tag="IW664_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0849671.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8528..8911
                     /locus_tag="IW664_002331"
     CDS             8528..8911
                     /locus_tag="IW664_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0849672.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8987..9118
                     /locus_tag="IW664_002332"
     CDS             8987..9118
                     /locus_tag="IW664_002332"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0849673.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9087..9323
                     /locus_tag="IW664_002333"
     CDS             9087..9323
                     /locus_tag="IW664_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0849674.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9401..10372)
                     /locus_tag="IW664_002334"
     CDS             complement(9401..10372)
                     /locus_tag="IW664_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0849675.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3304 a         2562 c         2119 g         3104 t
ORIGIN      
        1 agattggatg actcaccttc ccattgtgaa cactgtggat cgattcgtat tatccgcaac
       61 ggttcctata cgacacgtac tcagatgttg aaagtcaaag aaaagttgac tattttagaa
      121 ttaaaacgta cccgttttct atgctatgat tgtgggcaga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct catagaactg
      241 tacgaaaatc aatcacgtaa tctgaaaatc agaagaagta ccgtcggctc aagcgatatt
      301 ggaaactctt actcaaagat tccacgacat tggagccgct caaacggcat tatcatcgac
      361 tgtttaaacg accaattagt caaacagaaa tcgtcgatga accgttgtgc acaatggtgt
      421 cctgaacgta cagcgacacc ttccatatcc agagcagtcg ccacatcacg gggatggatg
      481 ccatccaaat taaaagcaac cacgccagta tgatgttcag gatcttgcgg tccgtaaatg
      541 gtgataccgc cgatcgcttg taatttaggt aaaacgtagg caaccagttc ttgctcataa
      601 cgatgaatat tctccatacc aatctcattc aagtaatcga ttgctcctgc aatgttcgga
      661 gtaccagctt caaatttcca tggcagctct ttccatgaac tatcgtaaag attgacaaaa
      721 tctatcattt caccgccgaa ttcgactggc tccatttgtt ctaacaattg acgtttgcca
      781 tataatacac cgattcctgt cggcccgcac attttatgcc cgctaaaggc gaaaaaatct
      841 gcatccaacg cttgtacatc aactgtcata tgcggtgctg cttgggcacc gactggacta
      901 tcctcaacta caacagcctg ttcaaccctc agccgtttca agcacaacaa ataaccttct
      961 ggatctggtt tgccgacctg aagatcatcg atcgtcacaa aggaatcaat tttttcctta
     1021 atcatcggat gtgcttcaaa aaaatagtct agctcccgca gttcagaatt gagactactc
     1081 cgattttcca gtgctgctga tccgctgctc aaaatgttca gcatccactg gtctcccaac
     1141 cgcttgactt atcccttcat aaaaggacag ttctgctgcc gtcccaacac cttgttgata
     1201 gtctgcaaaa ggaattttat agccgaactg ctccagtaaa taggcttcag taaatagtga
     1261 tgaatactct cgtaaaacat ccattcactg tcaatcatcg tcccatcaaa atcaaaaata
     1321 agtgctttgg tcatcttctt tcccccttac ccaaaacaag caaaagctgt gtcctgctct
     1381 ctctacagta tcagcataaa aactgccgtc tgaaaagtca aaggtgaaag aaatgtggcg
     1441 ctataatttc taggactgat agtaaatcat tctatcagtc cttttgagca taaaaaaaca
     1501 aaacatacag aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt
     1561 atgcctgatc ttcatcttat caaactatta ctaggaatca aagatggaaa cattcaaatc
     1621 actcatgtcg agaagaaaaa aatacattca atcaacagta tcgttgtttc tgccacgctc
     1681 attaaaacga ttcagccctg tcctcagtgt aaacaggtga atcaacatgg gctgatcgtt
     1741 aagaacggaa agaaaaaatc gctggtcctt ctcaataagt gtgcgaatca acggacttat
     1801 cttgctttgg caaagcaacg gtacctctgt cgtggctgtc acacctattt tacggcgaat
     1861 acctacattg ttgaaaaaaa ctgttttatc gccaaacaag tacattataa aatattggag
     1921 gaattgactg aaaaacaagc aatgacgacg atttctaaac attgcggcgt ttcttggtca
     1981 accgtttcgc gaacactcgc ttctctgctc cccatgacaa acgtgaaaat aaattggttg
     2041 cctcgctgtt cattggtaga tgaatttcgt tcattgaaaa atcaagtcgg tccctattct
     2101 ttttcttgta tggatggcga tactgggaaa ttactggata ttcttccttc acgtaagaag
     2161 aaagatctca tttcttattt tatgcaattt gaacgacgtg cacgtttaaa cgtaaaaata
     2221 ctcgtaacag atatgaacgc ctcttatgct ttgctgataa aagagtgctt tcctcatgcc
     2281 aaactcgttg tggatcgctt tcacatcgtc aagcacctca ttcgcagttt taaggatatt
     2341 tgactatgag tcatgaaaag cttcgaccga aacgatccaa tacaagccaa acactatcga
     2401 caagtgaagg ctttatctcg acttctgatc aaacgacagg acacgcttgt ttatgataaa
     2461 tggacaaaat ggcgtaattt tggttgggcc tatttaaccg aaagtgaagt cgtagaacgt
     2521 ctactttcta cttctgatga actacgaata gcttatgctt actatcaaga aattcttcaa
     2581 gctttttatg acaaagaagc ggacatcttc tttcagcttg taaaaacaat gcccaagtcg
     2641 gttccaagag aactgcatca tataaaaaaa gcgtttataa actatgaatc cggcattcgt
     2701 ttagccttag agttgcctta ctcaaatggc aaaatagaaa atctgcatac ccacatcaaa
     2761 gcattgaaac gaatagctta cgggttagga tgagaaccag aattttctta atcaatcatc
     2821 tgatcactta ttaaacataa aaaaacatct aaagcagaaa atcaattcta ccttagatgt
     2881 tcttatatca ctctatcagt cctatttgac aaagagccag aaatgtttgc cgactataca
     2941 ctagctttgt tgtgcaaaat gctgagtatc aatttccaca gggattgcta gaatatcttt
     3001 cagctgaatg tataaggcat cgctaatttt ctcttcccga tcgttcaacc gatccactaa
     3061 aatttctttg ccctccagcg tttgaaaaac ataccgttga atgttgccta aaataaatga
     3121 ttccttccat agaaccggaa tcttaacccc ctgaggaacc ggttcaaatt gaatcagctc
     3181 cggtcttaaa taataaatgc agcgattgcc cttagcggaa aactcgtggg tatactgatt
     3241 cagctcatac cattcgaaac gattatagtt gccgataaat tccgcaacaa atcggctctg
     3301 aggattctga tacaattcct tcgaggtgga aacctgagcc acccgcccag cttccatcac
     3361 aaaaatccga tcactgattc gcatcgcctc ttcttggtca tgagtcacaa aaatcatcgt
     3421 catccctagc gattgctgga tagcgcgcaa atcccgctgc agctgttttc taattcgggc
     3481 atccaaagca cttaatggct catccagcag caggactttc ggttcagtga ccaatgagcg
     3541 agccagtgcc acccgttgtc gttgaccgcc agaaagaaac tggacatttt tatctgcctg
     3601 ctccgtcata tccaccaatg atagcatctc aaaaaccttt tcctgaatca cctcaggact
     3661 cagcttcttt accttcaaac caaaggctac attttcaaaa acagtcatag ttggaaacaa
     3721 tgcatactgt tgaaaaacaa agccgatatt gcgatccttc gtaggcttat cggcaatatt
     3781 ctcattgtcc agcacaatct caccgctctt tggttgttcc aatcccgtga tacagcgcag
     3841 caacgtagac tttccgcacc cgctgggacc tagtaaagta accagctcac ccttttcaat
     3901 ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa aacagattga gatcatgtat
     3961 ttctaaaaac attttccgtc acaactcctt taagggcttc tttttcgact gcttctcttc
     4021 cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa gtcagcataa aggtagttaa
     4081 agcaattccc accataaaca gcaaatacaa aaccatcaca gcgctagcca aatggccgtt
     4141 ttcgttcatc cgccgcagca tatagatgcg taccgtctga aatcgaccgc caatcaacag
     4201 gttcgtcagc atgtactccc caaaaaccga agaaaaaatc atcagagaaa ctagggtcac
     4261 accgatacgg atgtttggaa acagcacctg gataataatc gtagacatcg gggcgcccaa
     4321 ggtttctgct gcatccacta attccttcag attaattaag cgcatctggt tgttaagact
     4381 aagatagaca atcggcaacg ccgtaataaa caatgcacct accaacacta caaacattgg
     4441 gataccagtt tcagcatacg ttcgaagcaa agccgttacc aaaatcactc ctggtaaagc
     4501 atacggcagc aggagcagct tttccagcca acgattcaaa cgagggaaat acaaatgaat
     4561 ccaaataatg gtgggaatca tcaacaacag aatcgtcacc aagaccacac ctgctaataa
     4621 aagtgagcga cccaccgcag caataaattc cctgtccgtc agcagctgtt ggaaccattg
     4681 caaggtaaaa tcactaggta aaagcgattt tgaccattta gtggagctgg catacaaaat
     4741 cgttccaata atcggcagca agtaatacag gacaaagcag aacaaaatcg gttttattaa
     4801 ctttttcacg tctgcaccac cttgcctttc ttttgaataa tcaacattgc cgttaccagc
     4861 aggacaccta aagcgactgc caacgcactg cctaaattag gcttggcgaa cacatctccc
     4921 gacactaaag ccgcaattcg aatcgttaaa agatttacat tactgctggt cagggcatac
     4981 gctgtttcat aggtccccat gccattagca aataagatga caaaggtgct ggcaatcgat
     5041 ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg ctgatgaagc gcccagcaat
     5101 tgtgcggact ccagccattc ctttttcact tccttcatgc tgggatacaa aaacaagacc
     5161 cctaacggaa tttggaaata ggtatacgtg atggtcaatc cccaccaaga atacaaattg
     5221 aagtccgcta gaaatggcaa aatcattttc agcacaccta cattccccaa caaaataata
     5281 aaagagaatg ccagcggaat cccagcaaaa ttagctgcca aattggaaac aaaggtgatt
     5341 ttctcctgca atttagtcga tagattcatt aagcataaac ataaaataaa cgtcccgatt
     5401 aacccaatca cactggagct gacagcaatc agtacgctgt taacaaaggc ctgtgtgtaa
     5461 tatttgttgc tgaaaatatc actgtaattg gccaatgtcc actggttagt taaatcactg
     5521 tgcaagctgc acagaaccat aaaaagcagc ggcagcagta aaaatgcaca gaagataaag
     5581 gaggcaggaa ccagaaccaa gcgttttttc atctctttca tcccctcatc aattcttgaa
     5641 tgtcctgcat tttctctgac ggtgcaattc ctaataacca gcaaaacaat ggagccgcca
     5701 gcagctgtgg tacagtttgt tccttcagct tttggcgttt atttgaaatc aaataaaaag
     5761 gaacttcacg atgtgcagcc aagctgccgc catgcagtcc gtgcgcatcc atcccgtgat
     5821 ccgcagtcac cacgacttga taaccttttt gcagccattc aggaaggtaa atagccaata
     5881 atctgtccgc ctgattaacc gctgctgcat attcatgaga atcggcggta aatttgtgac
     5941 cgacatcatc aatattcatc gaatgtacga ccaaaaagtc tggctgatac tgattcacta
     6001 aataatgggc atccgcaaat aaatgcgaat cagggtaagt gtcctcacta taaaagatgc
     6061 catgctgaat cagtcgctcc tcctgatgct ggatgcgatc aaccaaacga ttataggggg
     6121 cttgattgta caactcgctg taccagtggt aagcggcggc tcctgttttt ccgccagctt
     6181 ccttaactaa ttcaaacagc gaccactctt tggatcgctg agcatggtaa ttactataga
     6241 tgccattttc ataggctgga acgccggtca tcagtacttc gtataacgga cgtgaattgc
     6301 tgggcagctc actttttacc ttcaataagc ttgcttgctg gtgctcaact aaatgattca
     6361 tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc gtctagtata actacaatca
     6421 gtttgtcaag tctactttgc ataggtcagt acctccgcct gccaaagctc tggaagctct
     6481 acagaggtct tgttccaagc ttcattgtct tcaactggca ccgccgcttc ataagcatcc
     6541 tccggcagta atttttcagc tacttcttta ggcagctcta ccttgcgaat aggtcgcgca
     6601 tagccctttg ccaaattgat ttgcccttca tcagataaaa tatattcccg tgctagcttt
     6661 gccgcattgg gatgaggggc atttttattg attaaggttg tatacccgct aatgatggag
     6721 ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa tttggtcacg ataatttagt
     6781 ccattgaagt cccaaacaac tgccacatcg atttcacctt tttcaagatt tgctacgctg
     6841 gcatcaacgg tggacaaacg gcctttttct gccatttttt tgtagtaatt cagacctggc
     6901 tgaatatcct tttcgtcacc tccgttggca atcgccgcag ccaatacagc aaattgtgct
     6961 tggttggcaa tcgtcacatc ccccacgcta acttgatagt caccctcttg cagctccttc
     7021 cagctagtcg gtgcttgctt cacattctct ttatcggtga taaaggaaat ggttcccgta
     7081 tagcttaaca accaatcccc atcatcatcc tttgcccaat ccggaatttc ctcccaatag
     7141 ttggtcttat aaggtaaggt taactctttg ctttcagcca acggaccgaa gctgataccg
     7201 acatcaccaa tatcggcggt tccatttttt ccttctgatt caaacttagc caattcttct
     7261 gcactggaca tatccgtatc cacgtgactt aagccgtatt ctttttcaag gtcgttccaa
     7321 gtgcctaccc aatttgccca agtatccggc attcccaagc tggcaatctc tccttcttcc
     7381 ttggcttttt ctataatcgt atctaaagag tcgtctgccg cagccgattg cttcgttgct
     7441 tcacctgaga cgcagcctgt cagcaggata cttactgttg ctgcacaaaa taaaccgata
     7501 gtcatgttac gaattttttc attcattttc attcattctt ctcccctttc gataattcca
     7561 ttctaacaaa gctttgtaat gagagaatca gtttcttgaa aaggtttcat gaacaatgta
     7621 aaggttgtat atggtttgta aatttcgacg aattcccgtt aaccttctaa agcttttgcg
     7681 gtatgatagt agaaaaaagg aggctcccta tgcagtatta tgatcagcat ctccacacgt
     7741 atttttcctt tgattcagag gaaaaatttg aaaactattt agcctatcag ccggagtttt
     7801 tcgtttctac ggatcacttt gatttaaaca atccatctac cggaaaagat gacattccgg
     7861 attatccagc ctatataaac aaactggact ctttaaaaga aacctattcc accaaatttc
     7921 tgcgaggtat cgagattggc gttgttccag gacaagaaga gcggatcgtg gattacctcg
     7981 ctcagcatcc ttatgattta aagctggtaa gtattcatca aaatggccgc tttgattata
     8041 tggacgatgt ggttttaacc aaagatccca ttcaaactac caaggaatat tttgatcaga
     8101 tgttgcaggt cttgacggat tttccagaag gagatatttt gactcacttt gattatggat
     8161 tacgccgctt caccttcaca gcggaagaat tcgcggagca ttttgaggct cagctgctaa
     8221 cgattttcaa aaaggttgtt gaacgagaat tggcgatgga gttaaatgcc aagagcttct
     8281 tgaaatacgg caacgaagac ctttaccgtt atgccgttcc tttgtatcaa agtgtcggcg
     8341 gaaagctctt taccttaggc tcggatgctc atgttgccga agattatcag ctgggcttta
     8401 aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa gttgctagtc attcaaggaa
     8461 aagaacgccg cctgacgccg ctgccgattt tgtgattcct atataataga agaaagtggt
     8521 gaaaaaaatg agcaacctgt gggactacaa tcaagaggca cccatccatt atttaattgc
     8581 gcgacattgg gatgcgttga aaattgaggc ggtttgccga tccttgttag cagccgttcc
     8641 aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg cagcgggaaa aagttcaggc
     8701 ttattttgca gcctttaaag atcagccttt ggaatatttg catgcgcagt ttcatctttt
     8761 ttatcaagta gccgccccag atgactacaa cgatttacga ggacagctgc aattgacctt
     8821 tcaagcggat gaaaccgcct ataccgtttt attgggaatg gctcgccttg gtgatcaggc
     8881 gaaggtcgag tggcggattt tcgatatttg atggcaggat caaggcgttt cagttgcaaa
     8941 ccttcctctc tttcaattag aaaaaccgca gcaggaagaa gtttgtgtgc tggtcaccga
     9001 tgctggtgaa attcgtctcc gcttatttcc tgaccaagct cccctggctg tagccaactg
     9061 gcgctcccta gctgagcaag gcgtttatga caacacacct ttcggctcgt gtcattaagg
     9121 aatttgttat tcaaggtggc gctttggatg gttctggtga tgaagctgtt tccagctatg
     9181 agggcttttt cgctgatgaa gtgcatcgcg gactttatca ttttaatggc gccttggcct
     9241 taggggacca cggcccccac accaacggca accagttttt tattgtgcaa aacacaaagg
     9301 cgcaggctga cctgctaatg taaagcaagc atacttggag cttggaggac taccggagct
     9361 ggatggtcgt tatactgttt ttggacaggt ataccaaggg ttagaaatcg tagcagcgat
     9421 cgcagctcag ccgacaaaca gtgaagatgt accgattgag ccagtgttca ttcgacaaat
     9481 taacttcgaa aaaacatcgt aaaaagacac tcactgactt cagcaaaagc tctattcttg
     9541 agctggctgc agctagtgag tgtctttcat ttatccataa gataaagctt ggattcaata
     9601 aataaataaa tgttttggtt aaagctgtga ccagcaaatg aggaaccgtg cgagccagtt
     9661 gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg cgtgtctgcc aacggatact
     9721 ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg gtacggtagt aattctaacg
     9781 gtacgaaccg atcaagatct gccacaaaac aagcaatcgt cagcagctcg gtcaggttca
     9841 tattgatacc tggaaccaca ggaactcgaa ttcgcacttc gcagttagga tactcaatta
     9901 aaaagcgacg aatattcttc aaaatttgtt ggttatctaa gccggtccat tgtttgtgga
     9961 gcttgctggt aaacattttt acatccacaa acaatgtatc cacataggga gctacctttt
    10021 ggatttcatt ataagcacca caaaagctgg tttccagtac ggtattgatt ccaatgtatt
    10081 tcgatttttg caaaatttct ttagcaaaat ccgcctgaac cagtgcctcg cctccgctaa
    10141 tggtcactcc gccatccgag tgaaagtaaa aaacagcatc cttctcaatc tcatccatta
    10201 cctcttcagc ggtcatggtc tggccaaatc ctttttcaat ctgcttgctt tgtgattcgg
    10261 gaatcgaaca ccaggcacag cgtaatgggc agccctttaa ataaacaacg gtccgtaaac
    10321 cagcaccatc atgtagagaa ccctgttcta ttcttaatac tgttccttcc atatagattt
    10381 ttgaattaca cactagttta aactaaaaca cacaaggaaa aacttaccat ttaatcataa
    10441 aattagctca tataaatata ctaggatgta aaatagactg gtcaaaatct ggtcaaaaaa
    10501 gaggactaat acaggtagtc ctcttttttg ttattttaat attctaaatc attttttatt
    10561 ggtttattcc cttattcgat gtagactcaa atcactatat cactagatat caaacggcgt
    10621 catacggaat caaaaacatc aaatttgacc tgttaaatgg tcattgaata acattcaaaa
    10681 tcaataaaac ggagtcagac acggagtcaa aaacgctgag agtaaaaaat atatacagcc
    10741 ttgtaggtaa agtaccacag ggctgtatat tttttatttc agattacctt aaatgattcc
    10801 atttactttt tatctcattt tttcctacta gttagtatca aatactctcc tattttctca
    10861 ataaactatt tttggataca tggtatctga aacagctaaa accttgtcat tacagcaaaa
    10921 aatcacgtta tccagactgt agacaaagtc cattttatgg atgatgtcta cagtcttttc
    10981 ttttataata gatttatgaa aacgaatgca aggagtgata gctatgatgt ctaaaaacaa
    11041 tacgaatggt agaaatcaat ttgcgatgct tacgattgat gatctagtt
//
LOCUS       DACEKG010000088        10509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEKG010000088 DACEKG010000000
VERSION     DACEKG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.091"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IW664_002335"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IW664_002335"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0849676.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IW664_002336"
     CDS             1085..2005
                     /locus_tag="IW664_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0849677.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IW664_002337"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IW664_002337"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0849678.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IW664_002338"
     CDS             3311..4789
                     /locus_tag="IW664_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0849679.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IW664_002339"
     CDS             5066..7126
                     /locus_tag="IW664_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849680.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IW664_002340"
     CDS             7209..7778
                     /locus_tag="IW664_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0849681.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IW664_002341"
     CDS             complement(7953..8759)
                     /locus_tag="IW664_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849682.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IW664_002342"
     CDS             8897..10411
                     /locus_tag="IW664_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0849683.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3726 a         1752 c         2070 g         2961 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaa
//
LOCUS       DACEKG010000089        10433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEKG010000089 DACEKG010000000
VERSION     DACEKG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.092"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            87..305
                     /locus_tag="IW664_002343"
     CDS             87..305
                     /locus_tag="IW664_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849684.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(594..1106)
                     /locus_tag="IW664_002344"
     CDS             complement(594..1106)
                     /locus_tag="IW664_002344"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0849685.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="IW664_002345"
     CDS             complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="IW664_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0849686.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3559..3993)
                     /locus_tag="IW664_002346"
     CDS             complement(3559..3993)
                     /locus_tag="IW664_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0849687.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4003..4791)
                     /locus_tag="IW664_002347"
     CDS             complement(4003..4791)
                     /locus_tag="IW664_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0849688.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4788..5729)
                     /locus_tag="IW664_002348"
     CDS             complement(4788..5729)
                     /locus_tag="IW664_002348"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0849689.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5906..6493)
                     /locus_tag="IW664_002349"
     CDS             complement(5906..6493)
                     /locus_tag="IW664_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0849690.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6714..8027)
                     /gene="obgE"
                     /locus_tag="IW664_002350"
     CDS             complement(6714..8027)
                     /gene="obgE"
                     /locus_tag="IW664_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0849691.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8315..9325
                     /gene="gap"
                     /locus_tag="IW664_002351"
     CDS             8315..9325
                     /gene="gap"
                     /locus_tag="IW664_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0849692.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9410..10345)
                     /locus_tag="IW664_002352"
     CDS             complement(9410..10345)
                     /locus_tag="IW664_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0849693.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2888 a         2356 c         1809 g         3380 t
ORIGIN      
        1 atcacttccc tctttacaaa ccgaacaaat attcgtatac taaatatacg aacaattgtt
       61 cgcataaatt aaagaggtga gaattcatga aatcaatgag acgtcttggc ttagtattaa
      121 ctgttttgtt tttaggtatt attattggta atttcggcat acgtccagct ttacttgtgt
      181 ttgttccgtt atttactttg tggttcatgt tgtgggatga aaagaagtac cgtcaatctg
      241 agcggcgcag aattcatgaa gatcatttct atcatgagcc ctactacaga tccaggcaat
      301 cataataagg attgactgct aattattcaa aagacaagaa gttaaacttt tacgggggtg
      361 tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc cagtttttcg
      421 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
      481 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
      541 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
      601 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
      661 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
      721 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
      781 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
      841 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
      901 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
      961 attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata agcgtcacca
     1021 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     1081 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     1141 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgtcg
     1201 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     1261 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     1321 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     1381 ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     1441 caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg aataagaccg
     1501 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta gagagtttgg
     1561 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     1621 ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat aaaggctacc
     1681 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     1741 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     1801 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     1861 accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt tcagatgctg
     1921 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     1981 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     2041 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     2101 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     2161 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     2221 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     2281 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     2341 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     2401 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     2461 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     2521 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     2581 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     2641 cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg aaacaaggat
     2701 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     2761 aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     2821 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     2881 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     2941 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     3001 gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc catgctcaaa
     3061 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     3121 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     3181 gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca gatacggatc
     3241 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     3301 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     3361 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     3421 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     3481 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     3541 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     3601 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     3661 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     3721 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     3781 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     3841 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     3901 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     3961 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     4021 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     4081 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     4141 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     4201 atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg taacagctac
     4261 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     4321 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg cgatattaat
     4381 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     4441 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     4501 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     4561 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     4621 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     4681 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     4741 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     4801 tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt gttttcaaac
     4861 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     4921 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     4981 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     5041 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     5101 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     5161 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     5221 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     5281 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     5341 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     5401 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     5461 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     5521 aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct cggacgaata
     5581 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     5641 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     5701 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     5761 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     5821 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     5881 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     5941 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     6001 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga gtaatttctc
     6061 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     6121 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     6181 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     6241 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     6301 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
     6361 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
     6421 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
     6481 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
     6541 tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
     6601 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
     6661 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
     6721 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
     6781 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
     6841 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
     6901 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
     6961 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
     7021 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
     7081 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
     7141 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
     7201 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
     7261 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
     7321 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
     7381 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggt
     7441 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
     7501 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
     7561 tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc cggtgcagga
     7621 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
     7681 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
     7741 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
     7801 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
     7861 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
     7921 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
     7981 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
     8041 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
     8101 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
     8161 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
     8221 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
     8281 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
     8341 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
     8401 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
     8461 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
     8521 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
     8581 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
     8641 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
     8701 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
     8761 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
     8821 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
     8881 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
     8941 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
     9001 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
     9061 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
     9121 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
     9181 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
     9241 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
     9301 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
     9361 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
     9421 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
     9481 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
     9541 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
     9601 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
     9661 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
     9721 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
     9781 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
     9841 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
     9901 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
     9961 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    10021 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    10081 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    10141 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    10201 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    10261 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    10321 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    10381 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DACEKG010000090         9873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEKG010000090 DACEKG010000000
VERSION     DACEKG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.093"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..267)
                     /locus_tag="IW664_002353"
     CDS             complement(22..267)
                     /locus_tag="IW664_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849694.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="IW664_002354"
     CDS             complement(381..959)
                     /locus_tag="IW664_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0849695.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="IW664_002355"
     CDS             complement(1036..1566)
                     /locus_tag="IW664_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0849696.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IW664_002356"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IW664_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0849697.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="IW664_002357"
     CDS             complement(2423..2818)
                     /locus_tag="IW664_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0849698.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="IW664_002358"
     CDS             complement(2815..4227)
                     /locus_tag="IW664_002358"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0849699.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IW664_002359"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IW664_002359"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0849700.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5082..6542)
                     /locus_tag="IW664_002360"
     CDS             complement(5082..6542)
                     /locus_tag="IW664_002360"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0849701.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="IW664_002361"
     CDS             complement(6712..7869)
                     /locus_tag="IW664_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0849702.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IW664_002362"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IW664_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0849703.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="IW664_002363"
     CDS             complement(9336..9863)
                     /locus_tag="IW664_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0849704.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DACEKG010000091         9335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEKG010000091 DACEKG010000000
VERSION     DACEKG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.094"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..579
                     /locus_tag="IW664_002364"
     CDS             127..579
                     /locus_tag="IW664_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849705.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IW664_002365"
     CDS             593..1282
                     /locus_tag="IW664_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849706.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IW664_002366"
     CDS             1407..3758
                     /locus_tag="IW664_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0849707.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IW664_002367"
     CDS             3838..4233
                     /locus_tag="IW664_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849708.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IW664_002368"
     CDS             4558..5175
                     /locus_tag="IW664_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0849709.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IW664_002369"
     CDS             5216..5515
                     /locus_tag="IW664_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849710.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IW664_002370"
     CDS             5549..5716
                     /locus_tag="IW664_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0849711.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6324)
                     /locus_tag="IW664_002371"
     CDS             complement(5761..6324)
                     /locus_tag="IW664_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0849712.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6352..7032)
                     /locus_tag="IW664_002372"
                     /pseudo
     CDS             complement(6352..7032)
                     /locus_tag="IW664_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(7106..7387)
                     /locus_tag="IW664_002373"
                     /pseudo
     CDS             complement(7106..7387)
                     /locus_tag="IW664_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7849..9231
                     /locus_tag="IW664_002374"
                     /pseudo
     CDS             7849..9231
                     /locus_tag="IW664_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3207 a         1415 c         1768 g         2945 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt
     6301 tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt
     6361 tattcccatt aataccttga tttcagtaga caccgaaaag ccgaagagcg ttccatttct
     6421 tcggttcttt ttatatattc ctcgaatggt ctccatgccc ttaatcgtgg aagaggctgt
     6481 acggagactt tgataaaatt tatttcgtcg tttaataggt cgatggtctt gttctattaa
     6541 attgttaaga tacttcacag ttcggtgctc tgtcttagta tataaaccca cactctgtaa
     6601 ctttctaaag gcggagccaa gagaaggtgc tttatcggtc acaattgctt tcggctcacc
     6661 aaactgttta tggagtcgtt ttaagaaagc ataggctgct tgcgtatccc gtttctttcg
     6721 taaccagata tctaaggtta agccgtccgc atcaattgca cgataaagat aatgccaacg
     6781 tcccttaatt ttgatatagg tttcgtccat tttccatgaa tagaaggatt gtctattttt
     6841 cttcttccaa agataataga ggactttgct gtactcttat acccaacgat aaatcgtagt
     6901 atgacaaaca tttattccac gatcatataa caattcctga acttcacgat agcttagatt
     6961 gtaacgcagg tagtaaccaa cagcgacaat aatgacgtct tttttgaatt gtttgccttt
     7021 aaaatgattc attactctgt cctctctgtc ttttttctca attttacact aaaatagatt
     7081 ttttggaaaa ctttgcaaca gaaccgaaat gagtatagta tgaattagta atttggtttt
     7141 catctaagtt tagctttgat ttactaaaat tgatatctcg atacatgttc aatacatcta
     7201 atacaaatcg agaatcttct ttcggaaatg gctcagaaag atcactccaa atttcatcat
     7261 cataatgata ctcgtaacct tctctcaata tttcgatttt tctttcataa taagatctgt
     7321 tttggtcctc aaatttagcc aataactcaa attgatttac tagtgacaat ctctgaaaac
     7381 tgtccattaa atgtttcttc ctatcttgtt cattaacaaa aaattataag cccttacaac
     7441 gaatattata tattaaattt aattgtaggt gaacacgaat tttataaata tttatggtgt
     7501 taatgacaaa tgatatccga ctcctcttca tacgttctgg actatttgct aatagattaa
     7561 aatttcagca tctatttctg accataaata aatgagctgc cgaaaagctg ccaaatcaga
     7621 cttgaaaata aaggatcaaa aacttttaag tttctgaccc cgtaactcac acttcaaaat
     7681 cttacttaaa aaggagtctg aaagaaaaaa agagtattca aaattttttt ctaactagct
     7741 acatcacatc tatgcattga cactcaaaga accagaataa gtcatcagaa agcaaggggg
     7801 tgcggacata atcctggacg aaaaaataaa cttaggagga atgtccgtgt gttttcccac
     7861 aaagagcgtg taagggcaat tcagttattt ttaaaatacg attgctctta tgctgcaacc
     7921 attcgagaat tagggtatcc gtcgattggt gcactacgga aatggtataa agagtatttg
     7981 gaatccggag agctccatca ggagcaccga aaaaaatcaa agtattctga agaacaaaaa
     8041 cgaatagcgg tgaatcatta ttttgaatat ggtcaatgtt atgcacgaac cattcgcatg
     8101 cttggttatc ctaatagaga gtccttgcgg cagcggtgtg aagaacttgc accaggagct
     8161 agaaaactcc gaaaaagtgc ggtaaagtta acacaagatc aaaaagaagc tgtgttgaaa
     8221 aaattctata aaccgcaaac taataggaaa aatttagccg aatctggagg aattagtaga
     8281 gttactctat atcaatggaa aaacacggtt cttggaaagg attttccatt gtgtatgact
     8341 ttaaaaaatc aggaaaaaca aaaagagcta cttttaaagg aagtagaaga cttacaaaaa
     8401 caagtacacc aattacagct cgaaaaagca ttactcgaag gagcggctga actgctaaaa
     8461 aaagaaaaag gcgtcaatct tctttgtcta tcaaatcaag aaaaaacgat attgattgat
     8521 gccttacgaa atcagttcac cttgaaagag cttcttcaac agctccagtt tcctaaaagc
     8581 agctattttt atcaaaaaca agcacttgag aaaccagata agtattacaa agaacgtcaa
     8641 attaattatc acgattttca atcataactt ctgtgcgtat ggttaccgta gaattcacca
     8701 agctttaaaa aatatgggta aaaagctttc tgaaaaagtt gtacgtagat tgatgactga
     8761 ggaaaatcta tttgttaaat tttctagacg aaaaaagtat tcttcgtatg ctggggaaat
     8821 ttctcctgca caccctaatt tattaaatcg gaattttaaa gcaaagaagc caaatgaaaa
     8881 atggttaaca gatattactg aatttagaat tctcgcaggg aaaatctact tatcaccact
     8941 tgtcgattgt tacgatggtg ctattgttag ttggactatt ggaacaaagc caacttgctt
     9001 cactctatgt ctaaaaaggg ttattcacca gataattcag cgtgcgaagg tttctttgga
     9061 cgattaaaaa acgagatgtt ttatcaacgt gactggaaga atacaacaat caatcagttt
     9121 ataaacaaat tggatgatta tattcattgg tataataatc aacgaattaa gttatcttta
     9181 ggagggctaa gccctcttca atatcggaag aagcaaagat atatttgtta aaaaggtcca
     9241 aaaaaaatat ccgcaccccc ttttttttgt aaaaaactat tgcacaatac aagtagccta
     9301 cggctatcga aatttccata actttcgagc aagaa
//
LOCUS       DACEKG010000092         9317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEKG010000092 DACEKG010000000
VERSION     DACEKG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.095"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2122
                     /locus_tag="IW664_002375"
     CDS             <1..2122
                     /locus_tag="IW664_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849713.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="IW664_002376"
     CDS             2119..2739
                     /locus_tag="IW664_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849714.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            3432..3812
                     /locus_tag="IW664_002377"
     CDS             3432..3812
                     /locus_tag="IW664_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0849715.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="IW664_002378"
     CDS             3794..4708
                     /locus_tag="IW664_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0849716.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="IW664_002379"
     CDS             4948..5637
                     /locus_tag="IW664_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849717.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5758..5844)
                     /locus_tag="IW664_002380"
     CDS             complement(5758..5844)
                     /locus_tag="IW664_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0849718.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6489..7226
                     /locus_tag="IW664_002381"
     CDS             6489..7226
                     /locus_tag="IW664_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0849719.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7254..7793
                     /locus_tag="IW664_002382"
     CDS             7254..7793
                     /locus_tag="IW664_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0849720.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(8291..8752)
                     /locus_tag="IW664_002383"
     CDS             complement(8291..8752)
                     /locus_tag="IW664_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0849721.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            8885..9259
                     /locus_tag="IW664_002384"
     CDS             8885..9259
                     /locus_tag="IW664_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ0849722.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3601 a         1415 c         1450 g         2851 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat tcttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatatccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atataaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga aaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgaag actctcctcc
     1441 tcaagctcct tataaaactc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa
     3301 atacggaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta
     5761 cttcttgttt cggttattca accaataact gaataaagag acacaaactc ctacaagaag
     5821 ggggcaaatg aacagttcaa acattagata cacctccttc cagtcgcaag actgtcggat
     5881 aggcgacaag ttattatatc ttaaaaaatt aaatttgtgt ggtgttttta agtatcactt
     5941 gttttagggt tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag
     6001 ggaaattaat caagaattgt cttagaacgg cttagacaag ctttcagagt ctcacacctc
     6061 ctctttgaac aacggtttta gcaaaggagc cggaggcgac tgcgcagtat aatatgagag
     6121 catagcgatc acaccttttt agcgaccgta gggagcaagt tttccagttt agaaaaattc
     6181 gcgaaccttt ttggaggaaa aagtaggagt tttttaggcg ggaaattcaa tgtgctctta
     6241 agatatactg ttagatatac tgttagatat actgttaata ataactgtta gggattagca
     6301 accttatggg agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg
     6361 gataaattgt cacactatgg gggataaatt gtcacactat gggggataaa ttgtcacact
     6421 atgggggata aattgtgttt tacatcctct tatgtctatg ataacataat ttattgaggt
     6481 gatttttatt gagtaacgat atagttcgat ataataatgg attaaattct gtccctttaa
     6541 gaaaatttac ccctatagat atggatttat tttggggaat atgttctaaa atgaaaagaa
     6601 aaggtacaaa agaagaaatt tttacattta aacaaataaa agaattaatt gattacaata
     6661 taaatgcacg tggcgaccgt tttgctaatg atttaataaa aatgtcaaaa aaaatcatta
     6721 ctttgtcttt tatttatgaa gacgaaaaaa catatgaaca attaaatctg tttcaaaaat
     6781 ttaaaatcga taaagtgaat gaaacattat taataaaggt ttctgaagaa tttgaatata
     6841 tacttaattc aatcggaact aattttactc gtttcgaatt agaaaatatg acaaacttaa
     6901 agagtagcta cgttaaagag ttatatcgcc aacttatggc tcataaagac agaagatcta
     6961 aaaaaggtca ctggtttgta aaaatagaag attttagagc tctattagca ataccagaaa
     7021 gttatcgcat gagagatatt gataaacaaa tttttgccca agcgaaaaaa gaatttttaa
     7081 acgttaattc aactcaccct gctattttta aaacttttga aatcaaaaaa attaaagcct
     7141 acaaaggaaa taagatagga tctctgcaaa tttattttga agaaaatata ccttcaatta
     7201 ccttagataa ttggttagag aaataatacc taacaattta ggaggcaatt atcatgcaac
     7261 ccaaagaaac ctttacaata aaggaattat ctgaattatt tgaaataagt aggcaagcaa
     7321 tctctaaaca tgttgataaa ttagacccgt ccttattagc taaaaatgag cgtggttata
     7381 aggttgtatt acgttctgga gtattgcaac ttgctagcaa cttaggtaac caaaggttac
     7441 tatataggtt gcatgagtat gaacaagaaa acgataaaaa cgtttatata tcaagcaaaa
     7501 acgtgattgt aaacgagttg ctagataagt tgcgtgaaga aaatgaattt ttacgagaac
     7561 aactttccct caaagagcaa aataattcta atatgcaaaa attactcgac cagcaacaac
     7621 agctcaccct acaggcaaat caacagatcg aaaaactgca agaacagtta caattagctt
     7681 atacggaaga aactccagaa gatgaatcaa ctactattct ttctgaagaa acagaatcga
     7741 tagaaaaaca cacaaaaaag aagaagcaaa aatggtggca actttggaaa taactggagg
     7801 gaacattcat gacaagaaaa aatcttctat tcggttctat tttactagga attctaggct
     7861 tcagtccgat gttattgtca gtaatcaata aagggaataa acatggtgaa ttgaaattga
     7921 atccaaatta ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat
     7981 aaaaaagact gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa
     8041 gcacgcgctt ataaagcgct accaaaattt tatcatattt tataaaaaat aacaatccag
     8101 taaaaaatga ctggtaaaaa ttcacaaaga aacggaaaga agcaactaca gaaaatcaag
     8161 ccttaaaaag agctttttac ctagaccaat taaaaatcac aaagtgagcg tctctatcca
     8221 ttggtagagg ggctacttgg catagtgtac attatgtcaa ctatgcaatt aaataactaa
     8281 ctacttcata ttagcttaat aaaataatca aaatcactag gggaaaaaat attgtataaa
     8341 aaaatttttc attttttaaa taagaatagt aaatataaaa ataatttaag atatctatta
     8401 ctattaatcc agatagagca aaccaagtaa atattccgtg tgtaaagcta aaaaaattca
     8461 tttgaatcaa ttcaaataaa cctaatatag taacaaatat attaaaaata atagctgtca
     8521 ataccagtaa attattttgt ttatttttta aattatcttc atttaaaata gaatttatgt
     8581 tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt gttttgttat
     8641 tttcccactt tgaaatagtt tgacgtgtaa cattaaattt tcttgccaca tattcttgag
     8701 tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc attttttctc
     8761 ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg ccaaaaaagt
     8821 tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat aggagaaata
     8881 agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa ctagtggagg
     8941 aatagttagt gctacagaag ctttaaatct agatgtaccc gaagaccata ccgccattta
     9001 cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga gatatgctgc
     9061 tggcggcgga gatttcaact gtggggttaa tggatttaaa gtttatgcaa attactacca
     9121 tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag tacgatcagt
     9181 tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg gaaatactgg
     9241 ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta catttgaaag
     9301 gaaattcaaa atatgtt
//
LOCUS       DACEKG010000093         9156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEKG010000093 DACEKG010000000
VERSION     DACEKG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.096"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..269
                     /locus_tag="IW664_002385"
                     /pseudo
     CDS             1..269
                     /locus_tag="IW664_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            808..1008
                     /locus_tag="IW664_002386"
                     /pseudo
     CDS             808..1008
                     /locus_tag="IW664_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1495..1674
                     /locus_tag="IW664_002387"
     CDS             1495..1674
                     /locus_tag="IW664_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849723.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1720..2325)
                     /locus_tag="IW664_002388"
     CDS             complement(1720..2325)
                     /locus_tag="IW664_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0849724.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2507..2842
                     /locus_tag="IW664_002389"
                     /pseudo
     CDS             2507..2842
                     /locus_tag="IW664_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3007..3234
                     /locus_tag="IW664_002390"
                     /pseudo
     CDS             3007..3234
                     /locus_tag="IW664_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3360..4331
                     /locus_tag="IW664_002391"
     CDS             3360..4331
                     /locus_tag="IW664_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0849725.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4471..5784
                     /locus_tag="IW664_002392"
     CDS             4471..5784
                     /locus_tag="IW664_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0849726.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5827..6693
                     /locus_tag="IW664_002393"
     CDS             5827..6693
                     /locus_tag="IW664_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0849727.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6690..7538
                     /locus_tag="IW664_002394"
     CDS             6690..7538
                     /locus_tag="IW664_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0849728.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7552..9024
                     /locus_tag="IW664_002395"
     CDS             7552..9024
                     /locus_tag="IW664_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0849729.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9041..>9156
                     /locus_tag="IW664_002396"
     CDS             9041..>9156
                     /locus_tag="IW664_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0849730.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         2922 a         1525 c         1871 g         2838 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catttaaaaa gaaaagtgac
      361 agataggaat aaaaacttat taaatgtagt tatgatctgt tatatttcat ttctaatact
      421 aggtcttcca caagcagtgt ttcctgaaat gttttctgga gactggggta ttgtcagcta
      481 ttctatatta acattgattg ctatggtttt aggtacttca ttttataatg cttttgtaag
      541 taagcatgtt gaggaatatg aagtattaat gtacttaaaa gagaaaaaaa caagtaatta
      601 atatgagaac accacgcata gtttttattt tgagatattc tagaatgaaa cacattaaga
      661 acgaaagcaa agcaaaagac gccagaactt ctactacctt ctaagttttt tcatatttgt
      721 ttgttatgct atacatagat ctgcataatt agacttgcgc tgcccgttat tgaagtttca
      781 ggcagcgctc tttttggggg atcgataatg gaagaagaaa aaatatttga gaaacgctgg
      841 cagctagcat caagtgaaca aagagcaaga tacaataatt tgatcacctc ctaccccact
      901 gttgactaga catataagga aaagaaatac ttactttggc tgtgccagtt agatatagat
      961 acctttgaaa catttgaagt gatcctagat aaaattaaac agaattgatc agaaacaagc
     1021 gaagccttct ctcggttcgc ttgttttgct atttattagt tggctcaggg caactctaag
     1081 ctcgtttgaa agaaaaccga tggataatcc ccttgtttgt accactttag aatacatgga
     1141 tgtacgtatt gccattgact cctattagtt actgaaaaaa aatttaaaca agttgagtat
     1201 tgcagataaa tagctccagt gaggtgaata actgctccac tcaaggcaat agatagctcc
     1261 atccagggca acaaactgct ccactttcat atttaataga tgaataacct tctgtataat
     1321 agaaaggcat acctttcggt atgacaactt atacagaatg ttattttatt ggttcactat
     1381 cgaaaaattt tggagcttta tttttccggt atttctcaac gaacgattag tttaactgtg
     1441 ggaagctcca gacatacgat tcgagaagtt acttctcgag cagacaggaa gggggtgact
     1501 gaactaaccg ctgatatgag cgatacttgg ctgcaggaat atctttttcc ggaaaaacaa
     1561 acaacagcac gaggttacct tcctgtagac tgggaatatg ttcataaaga actaatgaaa
     1621 aagaatatga cattgaaact ccttcataga gaatatgggg tacatggaac atagaaacga
     1681 aaatgcacgg tagaaaagcg aatgaatata gtataaattc tacaaatcat tcgctttctt
     1741 tattttatat atggttggtc gagagagacc attttctttg gcaatttctg caactggaac
     1801 tccttgattt aacatgtcca ccacagtttt aaagactaat cgtttttgcg gatctgttga
     1861 gttctcagaa tattcaattg gtcgcccctt atatcgacca gcttttttgg ccaataatac
     1921 tccttgccgc tgacgttcac ggattttctc acgttcgttt tgtgcaatat aacttagcaa
     1981 tgataaaaac atatcgaaca tagcagtatc taggagttca tttccagtat taaaatctaa
     2041 aaactgtgca tctagtattt ttaatgctac ttttttctgt ttcataaagg taactgtatt
     2101 ttttatgtct tcataatctc tgcctaggcg atccagtgat gcaacaatga cacaatcacc
     2161 ttctcggata taatttagta atttttgaaa ctcaggtcgc tggatatctt taccactggc
     2221 tttttcttgg aataatttat caatgttaag gtcttccatc attgccaatt gtcgatctaa
     2281 attttgatct tgtgaagaca ctctaatata tccaattttt gccataaaaa ctccttttaa
     2341 aaaatgatat aatttacatg taaaatttac ttgtagtttt actgttatca tagcattttt
     2401 aaaaataaat tttaagtcta cttataattt actttggagg ttttgaatga atgaaattaa
     2461 gcgcaataaa aattcttttg gtgtttattt aggagagcct agctcggaac agttaaataa
     2521 aggtgaagct cgtcatgctc tatgtcgaaa tatattttat ggtagaaaag gcaaacttta
     2581 tcaaacttat tacgacggta tggaggaaca gctcaacgca ttaggattag taactaatgc
     2641 tatcatatat tggaattcac tatatttaga aaaagtcttt gaacaaatta aaacggaagg
     2701 gttcgactgt tccgaagaag tgttacgcaa gcaatttcca ttaattagtg atcacattaa
     2761 ttttgttggc aaatatagtt tccgctatga aaaagaatta tcgaatggaa tactacgtcc
     2821 gttaaatata gagaattatt gagaatgttc gtcttttggt tcctaccgtg cattttcgca
     2881 cggatgttcc agatactatt tttgaatgta taaaattgaa aacacaagaa tactaataaa
     2941 tcaacgattc ttgtgttttt aatttttcac taccgtgcat tttcgtttct atgttccatg
     3001 taccccaatc ttgaaaaatt aattaaagac ttagataaag ccattgacga cttagtcgtg
     3061 gtgctaccgg agtatcaatg tttaaccagt atcccaggta ttggtcctgt ttaggctgct
     3121 ggaatactcg ctgaaattgg tccaattgaa cgtttttctg atgaaataaa attgacaaag
     3181 tatgccggcc tatattggcg acagagtcaa tccggtaaat ctgaatacga aaattttttt
     3241 acgaaaagct tcacgtctaa tatctatttt aaaataaaaa tattcctgct aatttggaat
     3301 cgtttccttc ttttggtata ataatacctg aaattctttt gagaggagtt gttcgtgaag
     3361 tgaaaactac tatgaaggat gttgctgcgt tagcaggagt tggtgtgggt acggtatcta
     3421 gagtaataaa tggagtaaag gtgaaagagg ttaccaaaga aaaagtaatg gctgcaatta
     3481 gtgagcttaa ttatgagcct gatgaatatg ccagaggtat gaaaacgaat agtagcaata
     3541 caatagctct tattttacct acgatttggc atcccttctt ttccgagttt gcctactatg
     3601 tagaggaagc tctcagccaa aaaaattata agcttttttt gtgtaatgcg aacggcagtg
     3661 cggaaaaaga aaaagaatat atccaaatgg tcaagcagag taaagtagac ggaattatag
     3721 gtattaccta ttcagacatt gataaatatg tatcatcaaa tttaccattt gtcagtatag
     3781 accgccattt ttcggaacag gtttcttatg taacggcaga taattattta ggtgggcaaa
     3841 ttgccgctca agaactactt actcgtaact gtaaaaactt gtgctatgtt gggggtgttt
     3901 caccttaccc taacgaaaca aacaatcgta aagctggttt ttacaatatt tgtaaggaaa
     3961 ataaaatagc tgtgaaggta ttggatatgc ctgaaccaat tagcaattta gaggaacaat
     4021 tgctcgcttt tttttcggat aatcctgaaa ttgatagcat ttttaccatc aatgatttca
     4081 tggctttgga tgtgataaaa gcccaaaaaa aactagggaa ggaagcacct aaggattttc
     4141 aaattattgg ttttgatgga gtcaagctat cgttgcaaaa agactattta ctatcaacga
     4201 ttgtacagcc gatagaggaa atggccaagg tttcagtaga aacactcatg gcgatgattt
     4261 taaatgaaga accgcctaag cgtgtggtag taccggtttt ctttcacgct ggagagacga
     4321 ctaaagagta aaaaaccggt ttataacgat ttttttgaga taggttgaca gaaagtgatt
     4381 tcaatgatat cttaaaacca attaattgga aacgttacca attaattgaa aaataaaacg
     4441 gaaacgtttc aaaaaatagg aggaacatcg atgaaaaaga gaatgttagc aattgcagca
     4501 attttagcat taacagctgg aggacttgca ggatgtggca gtaaggagaa aagttcaggt
     4561 agcagtgatc caggagaaat taagatttgg gttcagtttt ctgatgaaac gcctgaagga
     4621 aaagcttggg cagagattgt gaacaatttt aatgatgaat acgagggtga atataaagct
     4681 gttactgagt atattcctcg tagcggaagc ggcggtggat atgaggataa agtaaatgct
     4741 gcaattacaa ccaataccct tcctgatgtt attacgttag atggaccgaa tactgcagct
     4801 tatgctcatt caggagtcat tgctccatta gatgattatt tgaaagatgc ggacatggat
     4861 gatgtattag acagcattat tcagcaagga acctacgatg gcaaaatgta tgcgtttggt
     4921 ttctcagagt ccagtgtggg tgtctattac aataagtcgc tgttcaaaga tgctggaatt
     4981 acagaagatc aattgccgac gttagaagat ccttggactt ggacggaatt tcaagcaatt
     5041 tgccaaaagc tgactgataa atacaaaacg cctgcaattg atatgcaaat ggcgtcggga
     5101 gacgaaatgc tgacctacgc atatacacca tttatatggt cgaatggcgg cgatgtcgtt
     5161 aatgctgatg gaaccaaagc cgaagactat tttaacggta aaaaatccgt tgaagcgttg
     5221 gaatttattc aagaattggt tgaaaaaggt tacaccacaa tcagtcctat tgaaaaagga
     5281 tttgaaactg gcaaatatgc catgatgctg agcggttctt ggacaatggc agatttagag
     5341 gaaaactatc aagagattga ttatggtatt ttgccttacc ccgtttcaga tgcaactggg
     5401 gagttagttt caccaaccgg aagctggcag ttagcaatga ctgagaaaac agagaaaaag
     5461 gatgcttctg cggcctttat taagtttgca accagtacag aatctagtcg tattatgagt
     5521 ttagggaaca gtgttctacc tatcagaaat tcaacagttg atttaattaa agatgaagtg
     5581 tcggaaggaa tgcaggtatt aattaagcaa aaccaaattt ctggacatgc ccgcccagtg
     5641 gttgtagctt atccgcaagt atctagagca ttccaacaag cagttcaaga tattagctac
     5701 ttcgaggaaa acccagatgt acaaaaagta gcagatagtc gcgctgctga aatgcaacag
     5761 gcaattgata cttcactaaa atagaagttt ctaaaggggg ccaatcaagt gttaaaaaaa
     5821 ccgacaatga aagacaattt gataggttac ggctttctag cgccagcttt gctgctgctg
     5881 acgatttttc tgatcatacc cgtaatcatg gtaatttatt atgcctttac tgattattat
     5941 ttgttaatgc cagatgctag acaatttatc ggcttagaaa attttaagcg actgttcgct
     6001 gatccaattt ttgtaaaaag tatttggaac acggctaaat ttgttgtttg gattattccg
     6061 gtccaaatag gggcagcttt aggaatggca cttattatca acaaaaatcg taaaggaaat
     6121 atctttttta aagtagcctt ttttgcgcca gtcgtgatgt ccttagtcgt tatttcgatt
     6181 ttatggttgt acttgttaaa tcctaatgaa ggtctggtta atacgctatt tgcaaagatt
     6241 ggaattgctt cgcagccttt tttgactagt cccaaacaag caatgtatac gcttgtgttc
     6301 gtatcggcat ggcaaggtgc aggatatcag atgctgctgt ttttaggggg aatgcaaaat
     6361 attccgcaag acgtttatga ggcagccgaa attgatggtt tttctaagtt tcagcaattt
     6421 cgctatatca ccatgccatt attaaagccc acggcgattt ttattttact taccacattg
     6481 atttcggcct ttaaactaat cattcagcca atggtaatga ctcaaggtgg accaatgaat
     6541 tctactatga caatggttta ttacatttac caaactggat ttacagaccg tatggtaggc
     6601 tactccagct ccattgcgtt aatctttacc actatgattg gtttaattac aattgctcaa
     6661 agaaaattgg taaaggagga tgatccagca tgaccaagaa atttggaccg ctaatgatcg
     6721 tagaatattg tttgctgttc cttttagcgg cattattcat tttcccgatg ctgtggatgg
     6781 tagtttcctc tatgaagcct gaagcagatg tctatcaaaa tttagcttcc ttcaaagctt
     6841 ttttacctag tttgaatcca gctaattggt ttaaaactta tcaagaggtt atttcccggt
     6901 tcagtgtggg aacttattta ttaaacagta ttttttatgg tttaaccttt gcctgtgggt
     6961 caatcattgt aaactctttg gcgggttttg cctttgcgaa ggttaatttt tcaggcaaga
     7021 aaattatctt tggagctttg ttagctctgc tgattgtccc gatggagacg gttttaattc
     7081 cgcaatttac catcgttaat tcactaggct tagtgaatac tcgcttggca gtgattttac
     7141 cagcgatggc cagcgcgttt aatatctatc tgtttagaaa tttctttatc gcgattccag
     7201 aagaaatcat tgaatcagcg aaattagatg gtgcaaatat tgttaaaatt ttctttcaca
     7261 tcatgctgcc aatgtctaaa ccagccatag ccacagtagg tgtcttatcc tttatctcca
     7321 gctggaatga ttatatttgg ccgttaatgg tcttaactga cagcagcaaa ttttcaatgc
     7381 aagtggcaat tactacgatc aatactacac agccagttta tatcaatcaa gtgatggctg
     7441 ttttaaccat tgcaacgatt ccactgatta ttgtttatat cgttgcacaa aaatatattc
     7501 tgcagggact tggcggttct ggaacgggta taaaatagga gtgaatagtg aatgttgcaa
     7561 aaacttttaa aggccaatga gtttattgag aaaaatgctc aaacagtcaa tcaagaatac
     7621 cggaataaat ttcatttgat ggctccgatt ggctggatga atgatccaaa tggttttgtt
     7681 tattatcaag aggagtatca cttgttttat caatattatc cttacgacag catctgggga
     7741 ccaatgcatt ggggccatgc taaaagcaag gatctaattc actgggagca tttgccagta
     7801 gcactggcac caggtgaaag ctatgacaag gaaggctgtt tttccggcag tgccatagaa
     7861 aaggatggca agctgtattt gatttataca ggacacaatg ttgtggaagg gcaagttcga
     7921 caagttcagt gtttggcagt ttcagaagat ggcattcact ttgaaaaata tgccggaaat
     7981 ccaatcatcg cagaagatca cttagcttct gttgcaacga ccgaagattt ccgcgatccc
     8041 aaagtgtttc agcgaaaaga agactatttt actgtagtcg ctactaaaac tgcagatgct
     8101 cgtggacaaa ttgtgatgtt ccattccaaa gatttactaa cttgggagtt ttattccgta
     8161 ttgctggagg gaactccaga acaaggcatt gtatgggaat gcccagactt gtttgaacta
     8221 gatggaaaag aagtcttgat tctttctcct attcaaatga ctccacaagg caatgcttac
     8281 caaaatacga actcaactgt tgctttcatc ggtactgtcg attggcaaac aggcaaattt
     8341 ctagtagaga attttcatga aatcgatggc ggtttagatt tttatgcacc ccagacctgt
     8401 gaaaacgaag caggccaaag aataatgatt gcttggatgc aaatgtggca gcgtaaaata
     8461 cctacgaatg atctgggcca tggttggtca ggttccatga ccttgcctag agagctgagg
     8521 gtggctgata atcaattgtt gcaacggcca gtttcatcaa tttatcaagt aattgatccg
     8581 caggaaagga ttgaatgggc gaagactgaa gagaatcgct tcttactgaa ggacttagta
     8641 tatgaccagc actatttacg cttggaaatc aatttaaaac aggcaacgaa actagaactt
     8701 cactatgcga agggacccga ccatacatta ctgcttaact atgatgttga acgggagtgc
     8761 tttactgctt ccagggaaca catgccgtat gatttatcag gagctgaaaa ggaagtgttg
     8821 aaaaagcgca gtgtgacgat tcctttggaa aataattgtc taaccatcga aattttccgt
     8881 gatacttcag ctattgagct ttttttaaat gatcgtctga ctatgagtac aactttttat
     8941 gagacagaaa aaggacagga ttttcttttg ctggcagaca gtgaagtaac cttttcattg
     9001 gaaagcggct taattaatct atagaaggga gagctcagtg atgatttatg gttcaatcga
     9061 agcaggcggc acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag
     9121 agtgagcttt ccgacaacga cacctgagga aacaat
//
LOCUS       DACEKG010000094         9023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEKG010000094 DACEKG010000000
VERSION     DACEKG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.097"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..731
                     /locus_tag="IW664_002397"
     CDS             51..731
                     /locus_tag="IW664_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0849731.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
     gene            complement(1347..2387)
                     /locus_tag="IW664_002398"
     CDS             complement(1347..2387)
                     /locus_tag="IW664_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0849732.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2736..3062)
                     /locus_tag="IW664_002399"
     CDS             complement(2736..3062)
                     /locus_tag="IW664_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849733.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(3049..3837)
                     /locus_tag="IW664_002400"
     CDS             complement(3049..3837)
                     /locus_tag="IW664_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0849734.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(4205..4495)
                     /locus_tag="IW664_002401"
     CDS             complement(4205..4495)
                     /locus_tag="IW664_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0849735.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4631..4810)
                     /locus_tag="IW664_002402"
     CDS             complement(4631..4810)
                     /locus_tag="IW664_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849736.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4807..5157)
                     /locus_tag="IW664_002403"
     CDS             complement(4807..5157)
                     /locus_tag="IW664_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849737.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(5150..6475)
                     /locus_tag="IW664_002404"
     CDS             complement(5150..6475)
                     /locus_tag="IW664_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0849738.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(6644..7228)
                     /locus_tag="IW664_002405"
     CDS             complement(6644..7228)
                     /locus_tag="IW664_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0849739.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(7439..7642)
                     /locus_tag="IW664_002406"
     CDS             complement(7439..7642)
                     /locus_tag="IW664_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849740.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(7652..8074)
                     /locus_tag="IW664_002407"
     CDS             complement(7652..8074)
                     /locus_tag="IW664_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849741.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            8388..9002
                     /locus_tag="IW664_002408"
     CDS             8388..9002
                     /locus_tag="IW664_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0849742.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT         2823 a         1619 c         1383 g         3198 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat accgtaaggg attttattct ttatttaaaa ctttgcaaca
      781 gaaccgtccg ttgcgggtaa taccgtaaca ctgaagggca cgcattaagg cgtcatctta
      841 atgctttcct gtaggacact gacggagatt accagtctcc cgagccttac aggggccttt
      901 cttttttttg tattttcaat tatcacaatt tatcgtccaa aagtgcagaa cgaaaatcaa
      961 ttcatcacaa gcatatcaga caattaaatt taaaacaact aaaaatatac aaaaaagtgt
     1021 aattgaatat tagcagcact gcctgaaaag aagtaatatc aggcttttca gcttggggaa
     1081 ataagtgaat tacctctctt catgaattat tataactcaa aaaagcacat atgcaaacac
     1141 tagttcccta tttttttgaa tgaaaaataa ctaaaatatc taacgattat gactttaaaa
     1201 cctacgtttg tgattgtttt cataaacttt taatctaaaa actccggaat aatagctatt
     1261 tatttgtaac atttgtaata aaattaaaat cataaaaaag ttctaattta aaattgatca
     1321 tttttatagt gatcgctttt tatcaactac tctagccaat tttccgtact aactgttggt
     1381 aattcgttaa atgtttctct ttgttcgttt gcaagctttg ccgcaatagc tttttcgaca
     1441 aaccaatttt ttacataaac gaaaattaga ttctgcaaat tgtttacttt ttcagttttt
     1501 tgtttttgat aagctttcag caaagtcata tataatcctt tgtcagcatc aattccataa
     1561 gattccagtt cttcataaac aacaactgtt tgctctaatt cttcagcttt tttctttgca
     1621 ttatgaatag tttggacggt tttttgagcg tcttcgtaag tactagaaaa tgtttttatc
     1681 aagtttaata cattttctgg tacaaatgta tttatactgt cctctttcat gatgtcgacg
     1741 aagttatcta gtaaaacttg attttgtaac tgatcagttt cagtgtctat tatgtgtcta
     1801 ttagtgtcta tagaattaga atagaataga ttgtctgcga ttttcgcaat tacggttgag
     1861 tccttgatat gactggattt tgccgtattt gcgattttcg caattacggt ggtatcattg
     1921 atttctcgcg gttttgccgt atttgcgatt ttcgcaatta cggtggtatc agtattgtct
     1981 tcctcttcca tgattttaaa gacgtcttga ttcgttactt ctggcttgcc taagtacaat
     2041 aaattaggca tggattcaat cttaccgtca cgtttattta ctcttcctct cttctgtttc
     2101 agaagacctg ctttttctaa ttcctttttg attttagata ctttaccttc gcggcattgt
     2161 aacaatttca ttaattcttc aactgtgaat atgaaaaata tattatcatc agaatctacc
     2221 cagttatttt tgactgaata atcaaatcga tctttaagta gcatataagc aatttttgca
     2281 tcatttgata gatccatgta tttttcgtta gtaaaaaata ccttaggcat ttgataatat
     2341 ttttcacgat aaatttcttt tgatttgtaa taattaaaat cattcatagc caatcctctt
     2401 tctgtaggat agcgttttgt tcaaaactat gttacaatga attaaacaaa tatatctatc
     2461 cactatatat ttttctactg ctgataagtc gccaaacttt gttaaagcag tagtcttttt
     2521 ttatcttttt ttagtttacc ttaaaaaata aattaaagca aaccgctcta tttcaaggtt
     2581 taagctcata gtaactatat cacgatatta caataatatt atatcacgat atcgtaatat
     2641 tacaatatga agataagcga cttatcagct ttgatactac tgatttttat aaaaaaaata
     2701 tcacgatatt atgatatcgt gatattgaat gtaaattaaa ttttattttg tttctttgct
     2761 agtttatatc cttcaatcaa gtattcttca ggtatggact ttaatagcgc atcttctata
     2821 acgtctggtt tacttttgtc tgattcagca atagtcgtcc aatatttgag tgctgacatt
     2881 agaattggat ttatacgaat gctatctgct gttcttacac tacttccttg atcttttatg
     2941 actttactta tatttttctt ggttaattcg atttcttcta cttcttttga tttaaaattc
     3001 tttttatttt tatttcctag taatctactt actgcagctt gactgcgatt atcttctgcc
     3061 atcaaaatca ccaacttctt ttaaatattc ttctgtaaat gctttatagt catcttcagc
     3121 tgttgagggt ttattaaaat tgattagatc tgatccagca gcttgcgctt cgtcaacggt
     3181 tattgtccta cgaatataag ttttgaaaat ttttgcacca atgactctag aaatttcggt
     3241 tgctgcagac gtcatttctt tagaaatgtt ataacgctta ttatacttag taaatagaac
     3301 tcctcgtact tcaagcttgt cgttcgtata ttcttttacc gtttcaatag tttcacttaa
     3361 ctttattagt ccttttaatg agaaatacgc agcttcaact ggaatgataa tttcatcaga
     3421 aacagtaaat gcgttgactg taagcattcc taaacttggt ggacaatcaa ttataatata
     3481 atcatagttg tccttgattg gttgaagtac tcttttaagc ctgtgttcac ttcctacttg
     3541 agtaaattcc ctctctgcag aagaaagggc tatgtcggct ggcaaaacat caattccttt
     3601 atagtccaga atagcatctg taatgtcagc ctcccctttt aatacttcat aaattgtagg
     3661 ttggtttgtt ccatctatat cgaaacctac attatctgta gcatttccct gaggatcaat
     3721 gtccactaaa agtacatttt taccgtaaac aaatttcaaa catgctgcta aattaacgct
     3781 agtcgtagtt ttagaaactc cacctttttg attggctact gacaatattc ttgccattaa
     3841 aatatccccc attcatctta tattataata taattatatc ataatattgt gatattgcaa
     3901 tatcgtatat gcattatatt gtaatattgt gtgattattt tgttttaaag atatatatcg
     3961 gttcggatat aaagattatt catatgttat tcgaaattaa aataatcact ttttattact
     4021 ttaaaatttt ttattaatga taagaaatat gttttgcgag cagcaactag aataatttaa
     4081 acattactaa actgtctata ttgttactaa taattccctt cttatagtgc attttcttag
     4141 aaaaaaacaa tattacgata tcatgatatt aagatatcgt aatattatac aattgtgatt
     4201 gatattatat atgttttttt atttttttat catgtttaat cttgaaggaa tcatattctt
     4261 tttctaaaga caggagcttt tcataaacat cagacatgtt taaactagag gccaggctta
     4321 aatcataaac gaatgaagca ggtaatgatt ttattctacg ttgtcgggtt atccagcttg
     4381 caaccgttcc ttgtgggtag gcatataact cacaaaattg ttctacagtg attcctaaat
     4441 tcttgataat gtaaacattg atcgggtgcg agtaaacaaa tgtattgata gacatggtgc
     4501 gtaagcctac tttcttttat tattgcgttt gcaataatag tttaacacac gcattattct
     4561 agtatctact cctatttgta aaaacaaaaa aggattcata gagataactc tatagaatcc
     4621 gtaaaatacc ttatatatat aacgggaaga tataaaacct aaaattatga taacctgctg
     4681 cagcttctac atagaaagag aagcgctcaa caccaacctc atttatcaaa cgttggccat
     4741 aaccccaaat ctcttctgat agctgatcaa aggttatata ctttagctca tattgctgga
     4801 taatgttcat tctaatcgac tccatttatt atcctggtaa aattcgatat gacgtatttc
     4861 gtcgtagtga attttgtctt ttccaacgta gatccccagc tcatcactac ctaagatctt
     4921 gccaatgata tcttctggat agtatccttc agcgtttcgt tcttcttttt gaatagatac
     4981 tgatctattt tttatgactg cagcgttcaa tacttccgca atctcttcag tcgtcatttg
     5041 ttcttttgct aaattttgac tggttcgttg ttcattgtcg gcatcaattt cttcagtatg
     5101 atcagataaa taaaaccctg cccactttaa acgtccacga tcattgtaat caatcatttt
     5161 taattccccc atttccgccc gcatgaccac caacaagtgt gcttctagta atgcttctag
     5221 cgccttctaa cattgaagaa gcatgtacaa tcgacgtaaa accatatttt tgccgaatct
     5281 tgtcaataat tttatctaaa ttttcttcgt caatttgctt ttgtggatca ctaaataaat
     5341 ccagctgaag cgaatcggtg tacacaagct tgctataggt tattccaaca tgccttactt
     5401 cttgtccttc ataatactta tcaaacaaga aaagacaata attctgcaat tcctttgtat
     5461 tatctgtgat agggatcttc atctggtgtg aaaaccctgg tctcgtttct aagatcgacg
     5521 tgccaatgtg taaatggaca caacctgttt tacagttatg ctgcctaatg cgtatcgcta
     5581 cttgttccgc catttctttt accaccagct cgatttcatt tcttctagta taatctcgtg
     5641 gtaacacttg tgaatttcca tatgattttt cctttgtcgg ttctggtggg attgctatat
     5701 ctgttcgatc gatcccatta gcatggaagt acagctgtaa tccaatcaca cctaatctat
     5761 tcttaatcgt ataaggattc cagttagcaa ggtctctaat gctcaaaatt cccatttggt
     5821 tgagcctttt tttcattcgc gaaccaattc cccaaaagtc cgtcatttcg ggaatattcc
     5881 atactttctc aggaacgttt tcgtacgtcc attccgcaat aaatccttca ttatgctttg
     5941 cttcattgtc taaggctaat ttagccaaaa ggggattatc gccgacgcct accgtagcaa
     6001 tcaaacctaa ctcttcttta atacgttctt ggatcatttg agcgagcttc ttccttcgct
     6061 gacttcgtgt ttcttcagtc gtaaaaagat tcagtgattt tgtcactttt aaaatcgatt
     6121 catcgatcga gtaaatcagt agatcttcat cagccacata tcttctgaaa atattattta
     6181 cctgcatatt tcgcttgata tataggttca tacgtggtgg aacaacgtgt agtgttttag
     6241 gaaatggttg tggtaagtca cgtggtctac tcacatttgt aataccatac cgcttttttg
     6301 cttcaggaga agaagctaat atcaatcctg aaccagtatt atcagatcga ctcataacaa
     6361 caagttctgt tgttaatgga tctaaatttc tttctataca ttcgacactt gcgtaaaagc
     6421 ttttcatatc gattaaaaag taatcattta ctggctcttt tgaataatcc atcataaata
     6481 acactccatt cttccacatt atacaaacat atgttcgatg ttgtaaagaa gaaatagcta
     6541 aatatttgag aataagttat cagtagacac ttacaaaaaa ttctcatact attgtcactt
     6601 ttctagataa atggcaaaaa ttttcagcat taaatgacaa aaactaatga gaatttgtct
     6661 ttccattttt ctcctccatt tttgccaaat aacgataaat ggtggttctt gagacgttcc
     6721 accgttccgc aatagtctta ataggtgtac cgctatccac taaagtcttc agtaattcca
     6781 aatcttgttc agacaatttt tcaggacgac caccaaaccg tcctcttgca cgagcagctg
     6841 ctcgtcctgc tgcggatcgt tccaggatca aattacgctc aaattctgca aatgccgcaa
     6901 acaaatgaaa catgagctgt cctgtagaac ttgatttgtc catcgtaata ttttcttgaa
     6961 tactatgaaa actcacacca cgctcattca gtcgattgac gattgaaatc aaatcttcca
     7021 tatttctacc taaacgatct aagcgccaaa cgacaagtgt atcgccagag cgaacaaaat
     7081 caatggccat ttctaatcct ggtcgattac tttttgcccc actcatatga tcagtaaaaa
     7141 ttttttcaca accaaattta tttaggctgt cttcttgtaa atccaaattt tgtaaaccag
     7201 tagatacccg ggcgtatcct attttcaaaa aaatcactcc ttcattaatt ttattgtaac
     7261 ataactttat ccatatagtt taactgaaca tagatattgt tatgggttta tgaacatgaa
     7321 atgaattgtt tttttgaaaa ataaagcaaa aaaagaaagt gtccagaaat gggtgatttt
     7381 atgaacactt tcaattagaa aaaaagaatg tcgactagac actctttttt taaaatcatt
     7441 atttttcttt ttgatatgtt ttttggtctc ttttttttct tttttcttca gaatcttcta
     7501 gattagaaat tgatttttga attttttgtg tctcctgtaa agttcgctga acttgtaaag
     7561 ctgattcttc tttcggtaaa ttaattttac ttagaatatc gctatcttga taaaaatttg
     7621 gtgtgttcac aaattctggc ataaaaaatc cctactttct agttaaggtt cctgaataag
     7681 tcgcaatcca attgtttcca gctgatttat aactttgtaa atacaatttt ccatagtacc
     7741 aataaccatt tcttttttct ttccaattcc gtgattgagg aggtctagaa ggccaaccaa
     7801 ctttatcaaa aatcatcgtt cctgtcactc gtatcgtttt agaagctgct cgagcaagta
     7861 atggagaaac tattaaataa ttttcagtaa cagttaagtc gttagttggg ttaccaatgg
     7921 ctattccgtt cctatctaaa ggagttaatt cttggttatt atctactaca gtagttggtt
     7981 catttgtact atcagcaaat gtaggaattg tagttccagt tacactaatt ccaatagtta
     8041 aaatacctaa agataaaata aacttatttt tcattgtcta tcacctcgaa atcaaaaata
     8101 agaaaacgtt ttcttaataa atcttaacag ataaatcaag ttatatacta gaaaattcat
     8161 aaaatatatt tttaatgtca taatcgttta aattttcttg attgacattt atttacaaaa
     8221 aatattgcca tttaaaaaga aaagtgacac atactatttc ctccatcatt agtttttgtc
     8281 tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg cttcctcgtg
     8341 tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg agagttgcat
     8401 acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat
     8461 tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact catcgacctg
     8521 ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg gaagctattg
     8581 atcgacttgg aagaaattat gacgagatca tcacttcagt caattacttg aagaagaaaa
     8641 atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc aatcctctcc
     8701 tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct gaacaagaac
     8761 ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca aacggcgtct
     8821 acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt cgccttgttt
     8881 acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt gctaaagact
     8941 acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat
     9001 agttgatcat gctgctttta tta
//
LOCUS       DACEKG010000095         8579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEKG010000095 DACEKG010000000
VERSION     DACEKG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.099"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(156..1109)
                     /gene="menA"
                     /locus_tag="IW664_002409"
     CDS             complement(156..1109)
                     /gene="menA"
                     /locus_tag="IW664_002409"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0849743.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1122..2225)
                     /locus_tag="IW664_002410"
     CDS             complement(1122..2225)
                     /locus_tag="IW664_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0849744.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2315..3250)
                     /locus_tag="IW664_002411"
     CDS             complement(2315..3250)
                     /locus_tag="IW664_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0849745.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3552..5486
                     /locus_tag="IW664_002412"
     CDS             3552..5486
                     /locus_tag="IW664_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0849746.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5943..6359
                     /locus_tag="IW664_002413"
     CDS             5943..6359
                     /locus_tag="IW664_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0849747.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6373..6939
                     /locus_tag="IW664_002414"
     CDS             6373..6939
                     /locus_tag="IW664_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0849748.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6962..7948
                     /locus_tag="IW664_002415"
     CDS             6962..7948
                     /locus_tag="IW664_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0849749.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8103..>8579)
                     /gene="scm"
                     /locus_tag="IW664_002416"
     CDS             complement(8103..>8579)
                     /gene="scm"
                     /locus_tag="IW664_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0849750.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2523 a         1659 c         1739 g         2658 t
ORIGIN      
        1 ctaagcgact ttttcacaac ctcctgataa acggtgttca aaagcttgca tctgcggtaa
       61 taagcccaac caaacgaaaa atataaggag ctatttttat ttggctgttc ttattacttg
      121 atgcagggca gttttttcac aacctcttca tgctgttaaa tggcttgcat aatgattgtt
      181 agtaatagcc ccaatacata actggcattg aataagacca tatttttgat ggaatagctg
      241 aaactgatag gctggggtaa cgtttgcttg aattgatcca aatgcttctt cacaattggc
      301 aaggtcaaga aggtgaccaa gatcggccat tcatagatac cgctcaacaa ccccattaaa
      361 atcacgaaat agcaagcata catcaatagc tggaataaga ctgttcccgc aggacgtccg
      421 atataaaaaa ctagtgtata gcgatgattt tcaatatcac ggtcaagatc ccgcaaatta
      481 ttagccaaca tgatattggc aatcgtaaac accaagggta aggaagctaa tacgattgct
      541 gctatagccc aaagatttcc taccaaatca aattgtccag atgaaaagtt gactgtcaga
      601 tcatagacat ctgaaccgaa tgtattgaca taaacgacca tggcaaaaat tccaaggccc
      661 atagtcaccc cactgaaaat ttcccctaaa ggcattcgag ataaaggaat cggtccgaat
      721 gtatagaata ctccaataaa acaacagata aatcccatca aaagtaaaag ccaacccgta
      781 ttgatcgtta gaataacgcc gataacaaaa acaaatgcaa tcatagctaa aatcatgttt
      841 cgaatcaata caggagatag atttctgcgg ccaatgacat tttcttcata cttatatact
      901 tcagaatgag ccttttcata atccatgtaa ttattgatag cggtagtcgc taaatcaaaa
      961 actagcattc caataaaaaa taaaaaagtg tttagccaat gtatctcatg aaaaaaagcg
     1021 atagagaata atacccctac cgcaaaagga aatacactag ccacctttgt tcgtatttca
     1081 actacttcta aaaagatttt tagtgtcatg gttcgtactc cttattttag ttcagaacgg
     1141 tcacccatcg tgtaatggga gtcctccgat aatttgaagg tgtctttgat tggatcggtc
     1201 acatatactt tatcgtcttt tgtcacgaag atcgcttccg tcccgttatt caattcgctt
     1261 tcgacgtatt ccagtccttt tttcacaccc attgaaaaga ctgctgtaga caacccgtca
     1321 ccatcaattg atttatctgt tatgatcgtg acacttgcga tatcattgtc gaacggataa
     1381 cccgtttttg gatcaaacaa atgatggtat tttttgccat ctacttctaa ataacgctcg
     1441 taaattccag aggtaactaa tgttttgttc cgttctttaa tagaacctaa aacagatccg
     1501 cgagccatgt tcggatcttg gatgccgact gtccagtctt ggttttcgcc tcttgggctg
     1561 tggcctaaga catagacatt accgcccaag tcaacgatgg cagtggtcac tccttgtttt
     1621 ttcaagactt tcactacttc gtccgtaatg taacctttgg cgattgcacc taaatcgatg
     1681 atcattcctt tttcttttag ataaacagtt ttttctttgt catttagttc gatcttatga
     1741 taatccacta atttcaatgc ctgatcgatt tcttcttggg aaggttttct ggcgtcatca
     1801 aaaccgattc gccataattg tgtgattgca ccaatcgcca tatcgaatcc gccttgagaa
     1861 tcttggctat attcgtaggc ccgcttgact aacgtgtaga cgtcatcaga aacttttact
     1921 ggtttgatcc cagcttgctc attcacttca tcgatctcag aacctttttg attgatcgtg
     1981 attttatcac caagttcttt tactcgatcg aaggcaggtt tcaatgctga ttctttgcct
     2041 tcatcataca cacggatacg aacgtaagtc cctaacaaga actgttcatc agaatatgga
     2101 tctttcaata tcttggtagc tgctgaactt tcagtggttg ttttatttgc tgagttgctg
     2161 cagccagtta gcaccataaa agagatgccc agtgtgatta aggaaaatag tagttttttc
     2221 cgcatctttt tcctctcttc attttgaaag gtgagacgac tgcctaatct ggctatcgtc
     2281 ccaccctcca acttttatta gtatgattat gtgattattt ttctgttacg atattatcta
     2341 cttcgattgt ttgagtgtcg cctttttcag ctgcattcac taattgttgt gcgtacatga
     2401 tgaatgaatg tgagctgtgt gtagcacctg taacaacttc tacattagca ggtgagccat
     2461 cttcttcgcc cattgctttg acgaattcgt cgttcagagt agggatgtaa gtttttggtt
     2521 ctgtaccgct ttttgctttc atattttcat tatattctgt atcatcttgt tttgatttac
     2581 cgtctttgtc gatataatca aagtttgatt cagttacttt gccgccagca actgtcattt
     2641 cgaattgcac acggtagccg ttagagtagt tcttttcttc aagcttgtaa gtgccgtctt
     2701 tcaaatcagc accattgtcg atttcgattg tatctgtgtt accagcttga gcagcttgga
     2761 tcaattgttg tgcgtagttt tggaagcttt cagatgagtg agtagctcca gttacgactt
     2821 ccacaccgct tgcgctttgt gcttttaaga attggtcatt caactcagga atgtattttt
     2881 ctgggtttgt gccagatttt gcttccatgt ttttgttgta ttctgcatct tttgtttttg
     2941 attcgccgtt ttcattgacg ttatcatatt ttgattcagt gattttgccg tcttttacaa
     3001 ccatttcaaa gactgcacgg taaccatttg aataattttt ttcttctaat ttatatgtgc
     3061 cgtcttgcaa atctccgcca gctacaactt ttgcagatga ttctgttgtg ctttttgcag
     3121 tcgaagattg tgctgtagat gattcagaag aagagtttga gcttccgttg tcatctgcgc
     3181 cgcaagcgcc taacactaga gctgaaaatg ctaatacggc aaatccagtt acaaaacgtt
     3241 ttttcttcat tttaataccc caccattcca tttctagtcc acaatttttc tttgtgattc
     3301 attatacatt ctaatctttt tagcctataa gtcaactatt tttgataaat aacccatgta
     3361 atatttcaaa cgttttcttt cagcaaattt cgtgaaaaaa cgctttattt taaaatttga
     3421 aaaaaatcca aaaggagtta gacaaaagaa cagaacatct atataatata tggagattgt
     3481 gtaataagta tcaaactaaa agaattatca tatattcatt atatagatag tctataagga
     3541 gcggatgatc aatggcaaag caaaatattg tcgttgtagg agcaggatat gccggtgttt
     3601 cagctacaaa atacttggca aagaaactga aaaaagaaga tgtagcaatt actctgatcg
     3661 atcgtcattc gtaccatact atgatgaccg agttgcatga agtagctggc ggacgtgtag
     3721 aaccagaagc aatccaatac gatctgcagc atttgtttgc tcgtcaaaag aatgtacaac
     3781 ttgtcacaga cactgtaatc ggtatcgata aagaacgaaa aatcgtcaag accaaattag
     3841 gttcctatcc ttttgatcag ctgatccttg gaatgggcgg agagccgaat gactttggta
     3901 cacctggtgt caaagcacac ggattcaccc tttggtcatt tgaagattct gtaaggattc
     3961 gggaacatat tgagaagaca gtagcaaaag ctgcattgga accagatgat gaactgcgta
     4021 aagccatgct gactttcgtt gtctgtggtt ctggatttac cggtattgaa atgattgggg
     4081 aactgatcga ctggaaaaat cgcctagcca aagattataa attagatcca aacgaaatca
     4141 cactgatggt cgtagaggca atgccgacga tcttgaatat gttggatcga aatgatgctg
     4201 caaaagctga acgctatttg cgtaagcaca acgtggaatt attattgaat gcaccaatcg
     4261 tagaagtggc tgctgatcat atcaaattga aagacggacg caacattccg actcacacac
     4321 tgatttggac agctggtgtc aaaggaactt ctgacgctgc tgactttgga ttagaatcag
     4381 cacgcggaca gcggttgatc gccaatgaat atatgcaggc aaaaggctac gaagataaaa
     4441 acatctatat cattggtgat ttagtctttt atgaagaatt cccaggaaca ccgacaccgc
     4501 agatcgttca agctgctgaa caaacaggac acacagccgc agcgaatatc gtggcttcga
     4561 tcaaaggcgg agaaaagcat aaatttaaag gaaactatca aggatttatg gtttctattg
     4621 gtgccaaatg gggggtagct aacctatttg ataagatcca tttaagcgga tttttagcta
     4681 tcgtaatgaa acatatcgtg aatttgaaat acttctttga tattcgttct ggttattata
     4741 tgttccaata tttaatgcat gagtttttcc atatcaaaga tgatcgcaac gtgactcgag
     4801 gacattcttc tcgttacggc aatgtcttat ggagtgtgcc acttcgtatt ttctatggtt
     4861 tagtttggtt cgttgaagct tccaaaaaaa tcattgggaa tggtgaagtg ttcaaaccaa
     4921 gtacttggtt tggtgaagga tcatggttca ctggaaacag tgtgttccct tggacatggg
     4981 aatacaatgt aacagatgtt gccagtggtg cttctgctgc tggtgatgca accacagctg
     5041 ccagcggagc aggggcaagt gccggagaag cagcgactca agctgcacat tttggtttga
     5101 gttatgcata cggtgaagaa ccaatggctg tgattgagaa gtcaccagac tggttcacaa
     5161 agatcatgca agtgatcatg ccaaacaatg atgtagcaat gttcttccaa aaattcatgg
     5221 tattctttga aattgccttg gcattagcat tgattgctgg attattcaca tggctatgca
     5281 gtgcgacaac gattgtattg gtgatcacgt tctgcttatc aggcatgttt tactgggtaa
     5341 acatttggtt catttttgtt gctttcgcat tgatgaacgg ttccggacgt gcgattgggt
     5401 tggatcgctg ggtaattcca tggctacaac gtacactagg taattggtgg tacggtagac
     5461 caagttccca gtacggcaag aaataaatag ataaagaacg ttatttgaat gcaagatttc
     5521 aatagaaagg ggctgagaca aaccgtcaca gcctcctttt tatgtaaaat taaaggaata
     5581 agatgcccga gataagcctc catgagcaag acacaggtgt cacaacctct gttacaacct
     5641 cttgttataa acggtgttcc agaagtagct tcttcgaaaa ataagccgaa atttcacaaa
     5701 aacttgaaaa gtaattttcg taaattcctt cttatttctt gaagttgccc acttctgtca
     5761 caacctcttg ttataaacgg tgttccacct gctgcttctc ggtaataagc ccaaagaatt
     5821 agaaaacatg gaaagctgtt ttctattctt cgttcttatt actcaaagca agacacaggt
     5881 gtcacaacct cttgttttta tgctaaaatt gggtgagtta atctggaaaa aggagaacaa
     5941 aaatgagcct taaagaattt gctgaaaaaa gtctgctcaa accttgggat gtttttatta
     6001 ttttgctttt gactgttctt tcttttttac cagtcatgat ttttggtctt cagcaaaaca
     6061 ccgcaacagc agaaaaagaa gcggtactgc gtgtggatgg cacagaaatc aaaacattcc
     6121 ctttgatggc tggagaagag agcaacacat atacttacaa agatgaacac ggagattata
     6181 acttgatcga gatagatggc gatcgagtac ggataaaaga agcagattgc gatgaccaga
     6241 tctgcgtacg tcgaggctgg gcaacgaaaa atggcgaaac catcgtctgt ttgccacata
     6301 aattagtgat cgaagtccgt tcaacagatg gaggtgacga agacagtctg atttattaga
     6361 ccttcacaca ccatgagtaa attacaaaaa atgatttata tttcgctact tgttgcacaa
     6421 ggagtgatca ttggtttatt ggaaaatatg atcccttatc ctttttcttt tgcgcctggt
     6481 gcaaaactag gcttagccaa tctaatcacg atcgttgcca tttttaccat gaaaaaacaa
     6541 gacagcttct tactactttg gctacgacta ttcctgacta ctttgctagg cggaacgatt
     6601 tccacattcc tttatagtat gagtggtgct ttgcttagtt attttggaat gttattggtc
     6661 aagcaattag gaccaaaacg tgtcagtatc atcggaatta gtgcggccgg cggtttcatg
     6721 cataatgtcg gacagctttt gatggcttct ttcattgcac aatcatggac agtcatgttg
     6781 tacttgccag tcctttcagt tttagggatt ttatcaggga ttgcaatcgg gatcgcagcg
     6841 aattatcttt tacgacatgt gcagacactt caacgattcc aaaaagatta tgagcaggga
     6901 accaaacaaa aatggagccc aatgtttacc aaaaaataat cgtcacacaa ggagtttttt
     6961 tatgaagcta catccaatgt gggaaaacta tcctgcttta agtaaagaat tgacagaaac
     7021 actaaaacgg atggaagaaa ctgtccgcct gaaaaacaaa cctgtcgaac aagcaatcaa
     7081 agaaacgatc catgccggcg gaaagctgtt gcgtcctgcc tatcagctat tgttctcaca
     7141 atttggacca gagcaagatc gagacaaagc catcgctttg gcagcttcta tcgaaatgct
     7201 ccatacggcc acactgatcc atgatgatat cgttgatgaa gcggatctgc gacgtggtca
     7261 gcctaatatc cgtagtcagt ttggtaatgt cgtttctgtc tatgcaggag actatttatt
     7321 tgtctgctgt tttaaactat tgtctgatta ctccacttcc ttgaaaagtc tccaattgaa
     7381 ttctcgcagt atggaaaaag tattgaatgg cgaattaggg cagatggatg atcgctataa
     7441 ttatgaactt tctgtaaaag aatatttgga aaatatttca ggtaagacag ctgaactgtt
     7501 ccaattaagt tgttctgttg gcgcttttga aagtggaaca agcgagcgct tcgcaaaaaa
     7561 agcaggagat atcggacttt ctatcgggat ggcctttcag attatcgatg atattttaga
     7621 ttatacaaaa gagagccagg aaatcggcaa acctgtacta gaagacatgc gtcaaggggt
     7681 ctattcttta ccgttgatct actctttgca aaaaaacaaa ccaaagctac taccttattt
     7741 agagaagaaa gcggcaatga cagaagaaga tgtcgacgcc gtacgcaaaa tcgttgaaca
     7801 tacaggaggc gtggaagaag ctaggaagtt agcggcctct tacacagaaa aggctttgaa
     7861 ggaaataaag aaattacctg ccacctctct tcgaacaaaa gagaatctct tttcccttac
     7921 ccaactgatt ttggatcgca aagactgaat agggaaaggt tcgatacgta aataaatttc
     7981 tcagaaggaa atctacgtgt tttttgacaa attgaattgt agttcttgta tttatgataa
     8041 aaagcaaaaa taaagagcaa cgtccgttag cgacgttgct cttttttatc ttgaatattt
     8101 ttttaccttt tcgatttctt ataactcata aaagtgattc ttaaaccgat tacaagtaat
     8161 ataacgcctg cgagtgttat ccaattattc gattgacttc cagttttagg aagtgctctt
     8221 tttgatttgt ttccattgtc attgcttgtt ttattagtta agttaaagtt cgtcctgtta
     8281 tccgaacttg tttttgagaa ggagacactt gcatgacgtg tatcgcttgt ggtagacgac
     8341 gtgtctgtac tatcgcttga agttgatgaa tctgagctat cacttgtggt agaggatgta
     8401 tctgtactgt cacttgaagc tgacgtgtcc gtgctatcac ttgtagtggt agatgaatct
     8461 gagctatcac ttgtggtaga ggtagtatct gtactgtcac ttgaagctga cgtgtccgtg
     8521 ctatcacttg ttgtggtaga tgtatccgta ctgtcgcttg aagctgacgt gtccgtgct
//
LOCUS       DACEKG010000096         8576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEKG010000096 DACEKG010000000
VERSION     DACEKG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.100"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(312..842)
                     /locus_tag="IW664_002417"
     CDS             complement(312..842)
                     /locus_tag="IW664_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0849751.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1024..1680)
                     /locus_tag="IW664_002418"
     CDS             complement(1024..1680)
                     /locus_tag="IW664_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0849752.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="IW664_002419"
     CDS             complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="IW664_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0849753.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2699..2917)
                     /locus_tag="IW664_002420"
                     /pseudo
     CDS             complement(2699..2917)
                     /locus_tag="IW664_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2948..4162)
                     /locus_tag="IW664_002421"
     CDS             complement(2948..4162)
                     /locus_tag="IW664_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0849754.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4503..4745)
                     /locus_tag="IW664_002422"
     CDS             complement(4503..4745)
                     /locus_tag="IW664_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0849755.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4777..5334)
                     /locus_tag="IW664_002423"
     CDS             complement(4777..5334)
                     /locus_tag="IW664_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0849756.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5347..5535)
                     /locus_tag="IW664_002424"
     CDS             complement(5347..5535)
                     /locus_tag="IW664_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0849757.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="IW664_002425"
     CDS             complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="IW664_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0849758.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            7344..7817
                     /locus_tag="IW664_002426"
     CDS             7344..7817
                     /locus_tag="IW664_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0849759.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7826..8053
                     /locus_tag="IW664_002427"
     CDS             7826..8053
                     /locus_tag="IW664_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0849760.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2607 a         1684 c         1274 g         3011 t
ORIGIN      
        1 ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt gatataggta
       61 caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg ttttccgctt
      121 gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg gggctttgaa
      181 cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca tggcggaaaa
      241 atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac agcttttctg
      301 tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata agtctgtgtt
      361 ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac cccatttcct
      421 aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct taccaactgc
      481 tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg ttttagccgt
      541 tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga attgcactct
      601 ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg agaaagttca
      661 gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc tttggtaaat
      721 tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca agctactcca
      781 ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac tacttttctc
      841 attttttctc ttttataaat acaaacagga atttatttct cttttttact cataagggaa
      901 caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct tataaaatca
      961 ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa ctgaaatttt
     1021 tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca taatccaatg
     1081 tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat aatcaatcag
     1141 ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc cttttctttc
     1201 tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt tctcagtgct
     1261 ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt ttgaaaagtc
     1321 tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat tgatacgctc
     1381 ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat gcacaactat
     1441 atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat gataaccatc
     1501 agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt tgaaagatac
     1561 taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc tcttataagt
     1621 atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt ccttattcat
     1681 attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat ttgtcttaca
     1741 acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat ccaaaattta
     1801 ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct caccaagtgt
     1861 ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg tatctccatc
     1921 ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca aagcttcctt
     1981 aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata ataatgcatc
     2041 ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg tggaaacaac
     2101 aatattcatt ctattaggca acggatgacc gatttcttca taagactttc gccccataat
     2161 aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta ttttccatgg
     2221 tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca aacctatcat
     2281 ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc accacatctt
     2341 aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt gtcttcatct
     2401 gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa aagttactta
     2461 tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag cccttgacaa
     2521 accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg ctgtttcacg
     2581 gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt agcaatattc
     2641 ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt tgggaaacac
     2701 ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc
     2761 atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg
     2821 gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta
     2881 atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga
     2941 atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt
     3001 cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca
     3061 cataggcata cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc
     3121 tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg
     3181 tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt
     3241 tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac
     3301 tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg
     3361 ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa
     3421 tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg
     3481 ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga
     3541 tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac
     3601 caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac
     3661 cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa
     3721 cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca
     3781 atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt
     3841 aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa
     3901 catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa
     3961 aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag
     4021 attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta
     4081 gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg
     4141 tattgatagg taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga
     4201 aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag
     4261 acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa
     4321 aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt tatttttaaa
     4381 gatatttttt cataaggaat aaagaatact tcatataaaa tatataaaaa agaaccgcag
     4441 cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt tcttcaattt
     4501 gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc accgatcaat
     4561 gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa agcttgtcca
     4621 attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga tgatccttta
     4681 ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc ccaaataaat
     4741 cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg gttcgttatt
     4801 ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg tttggcgtga
     4861 agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg tttcttgatc
     4921 tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt cgataacatc
     4981 taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt tgtaaatatc
     5041 ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt gtttcttacc
     5101 tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt cagccatatt
     5161 tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg cataaccgat
     5221 gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc ggttttctcc
     5281 tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt ccatctttgt
     5341 ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat aaaaaccgat
     5401 tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc caatcgttaa
     5461 ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct tgtatgctga
     5521 aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt agtctcttgt
     5581 atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt cttttgttcc
     5641 gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt tccctactaa
     5701 tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt tcgttgaacg
     5761 tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc
     5821 ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa cgaaagttcc
     5881 tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa cggcaaatac
     5941 tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa agaagcgaaa
     6001 tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg ctagaaatac
     6061 tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat tcattttcca
     6121 cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca gtatagatag
     6181 cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg atcataacgg
     6241 cgttttattg ccagtctgat actgaaataa caattgctta cttattctct atttactttt
     6301 gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag attatgctca
     6361 gttattatta caagattcta tttatttatt aaattttttc ggagaattaa aaaatacaat
     6421 aaataacttt agttacttta atgcgttctt actttatttt tatattatca tttttcttat
     6481 tcacttcttt ggtttatact attcttataa ataaaaacga ggtatgtcct atgatgaaaa
     6541 gattgtacta ttcattgatc ataacgattg gctatttgat tgtatcaaat ctaggaaata
     6601 tggtatttgg tatcagtaaa gaattcagtt ggactactac attatgggaa tctctattct
     6661 ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa ataaacatgt gccatctatc
     6721 tgttggcaca tgttttagga tttatggtac tttttattcg gcaatccatt ttatttttct
     6781 acttcttctt aatacctcta acacttttga tttataaact aatccaatgc atctacttgc
     6841 gcttattaag ctatttcagc tagatagtac caccccatgc gtcaatgctt gattttcctt
     6901 ttgtataaca ttgtataagc aataaaggac aaataagtgg tgattacacc aaaccattga
     6961 ataagaaaaa ttctgatacg taaataaaca tgaacttgaa aaaattaatg aatcggttag
     7021 ctactacgta gttgtctttt ttctcataca gttctgttaa cttacacttc tcttcgctag
     7081 ataataaaaa atcctccaac tgtattgtcc aatataatcc caggcatcta aactagcatt
     7141 atagaagaga cacacataga atctaaaata actggtttta aatgtttctt acaatcaaaa
     7201 aaagctgtta aatgcactcc acaccttcat actctatcat tttttataaa cgatatattt
     7261 ttattgtttt tcgataaaaa atagttgttt tacaataatt attctgctat attttagcta
     7321 tcaaagataa aagagggata aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt
     7381 taggtataag tgccgtattt attttgttcg gcgtactttt ttatactcag atcgctacaa
     7441 gcgaaaaagg attcaatttt gattggcaga ccacattatt tgtttctgtc atttactttg
     7501 cccttctcct agggatcgtt attgcttatt tcttattgaa actattgaaa aaaatcgaag
     7561 ataaccaagc tttttccact caaagcttaa acatactaaa aaaaatcgaa cgaaccattt
     7621 tgtttattgg tttcgccttt tttggcttac ttcctaaatt tatgaaagta gcggatttag
     7681 acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt attcattcct tttgcagtat
     7741 acacattaac aagcgtgctg gaaaaactgc ttgaacatgc gattttactt aagacagatc
     7801 atgatttgac agtgtgaggt gcgacatggc aattatcgtt aatttagatg tagtattagc
     7861 tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg ggcattacga tggcaaacat
     7921 ctctattcta aaaaatggaa aagcaaaagc catccgtttt tccactctcg aaaaaatctg
     7981 ccaagttttg gagtgtcaac caggtgattt gttgacctat aaaaatgact cgaatactat
     8041 accagaagaa taaaataaag tagaacaaac agtaaataaa aaataatctg aatgatagat
     8101 gtgtgagact tttagaagtt atttacgtct attattcaga ttttttattt cgattgaata
     8161 ccgttttatc tataaataag atatgtactg actcatacaa agggggcctt ttcttctcca
     8221 tgtactatca cttttcctga tacaactggt ttcctgttac gttgtagttg gatctgcttt
     8281 tagtataata ctttattcat tccaccagac tgatagcagt ttgagcagtc taaataatca
     8341 tgtagtatta caggccattc tgtttcatac cttgaagtgc ttaaaacttc agctaatggc
     8401 ccattgtgtt aactcttact ccattctttt ttagtaagct cccatatata ctgtaaagcc
     8461 ttctatatat tatgtcatgt tgctttttaa catttactat gtagttttga taaaatgatt
     8521 tgaataacag tgatggacaa ttctttttta tttttaacat tattttgata ataatg
//
LOCUS       DACEKG010000097         8466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEKG010000097 DACEKG010000000
VERSION     DACEKG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.101"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            393..1832
                     /locus_tag="IW664_002428"
     CDS             393..1832
                     /locus_tag="IW664_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0849761.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2127..2345
                     /locus_tag="IW664_002429"
                     /pseudo
     CDS             2127..2345
                     /locus_tag="IW664_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2594..2809
                     /locus_tag="IW664_002430"
     CDS             2594..2809
                     /locus_tag="IW664_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849762.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3026..3282)
                     /locus_tag="IW664_002431"
                     /pseudo
     CDS             complement(3026..3282)
                     /locus_tag="IW664_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(3469..4380)
                     /locus_tag="IW664_002432"
     CDS             complement(3469..4380)
                     /locus_tag="IW664_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0849763.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4399..5403)
                     /locus_tag="IW664_002433"
     CDS             complement(4399..5403)
                     /locus_tag="IW664_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0849764.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5400..7523)
                     /locus_tag="IW664_002434"
     CDS             complement(5400..7523)
                     /locus_tag="IW664_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0849765.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7528..8448)
                     /locus_tag="IW664_002435"
     CDS             complement(7528..8448)
                     /locus_tag="IW664_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0849766.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2433 a         1720 c         1562 g         2751 t
ORIGIN      
        1 tattgatatt tagtataatc ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa
       61 attggaaaac aaataggtgc aacaccataa atatgaaaaa attgggaaaa agtagaatgg
      121 aaatttgctt acgtctcacg agatagctca cactattaat ctgaagaaca atgaagtcga
      181 cttttagaaa ttgctagaag aaagtaaagc gaattatcac tagttactat agagtaagtt
      241 caaataaagc atctccaact tacatggtta catgaggata tcaaatcgaa attactcaag
      301 cttcctatgg ctagagtcgc gagaagttgt tgggcagtag acataggtta tattgttctt
      361 agctacacga tcattagaaa ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag
      421 tccaaaaaaa tcgctggtgg attttgattg ctatatctat gtttaccttc atgtccacat
      481 tggatggcag tattgtcaat attgcactac ctaccatctc agatgatatg ggcataccaa
      541 tgaatcaagc ggaatgggta gtgtccgttt atctgatgat tgtctgttct tgtttgttgc
      601 tgtttggtaa aattggcgat agccgtggga aaatcaaagt atttcggatt ggtacgctga
      661 tctttgtctt gggatcgctg ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg
      721 cacgggtggt acaagccatt ggtgctagta tgactatggc tacaaatgcc ggaatcatca
      781 cggaagtctt tcccatacat gaacgtggtc gggcattggg agcaataggt gcgtttgttt
      841 cattaggctc gattgctgga ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg
      901 cgtacatttt ttggatcaat gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat
      961 tcttaccaaa agatatcacc atgagcggcc atacaatcga tggagggggc tttgctgcca
     1021 ttgcggctac gattatcagc ttttttggag caatcttttt ggggcaagaa agtggctttt
     1081 tccatccagt cccccttgta ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa
     1141 aagttgaaaa gaaagcagcc tctccattga tttctttcgg tatttttaag aacaagattt
     1201 ttaccatgag cttgattaca gccttgttga ttttttcttc caacttcttt gtcaatgtag
     1261 tgattccttt ttatctgcaa aatgctcgcg ggttgagtcc tagctatgct gggctgttga
     1321 tgatggtctt tcctttgatg atggtcgtcg gggcgccttt aagcggctac ctgaccgacc
     1381 gggttggccc ggagattttg gtattagtag gcttgggctt attgtcggta acccaggtca
     1441 tgtatatgtt tttgtcagtg gactctcctt tgtggctata cattgtcgct actgccatca
     1501 tgggtgccgg caatgccttg ttccagtccc ccaataatac gatggttatg agctcggtca
     1561 ctatcgaaaa cttgggactg gcaggctcga tgaattcctt tgcccgaaat ttggggatgg
     1621 tcagcgggat ttcgctttct acgacggtct tatatcaagc gatgtctgct aagatgggcc
     1681 agcaagtaac taactatatc gcaaatcggc cggatgtctt tttatatgga atgaagatta
     1741 cctttttagg cagctttgtt ttgtgttttg ttgccttttt cctgactatc tggcgtatca
     1801 aaaaacaagg aggtattcag aaaaatgtct gaaaaaacca ctgttgaaga atttttggag
     1861 gcctgcgtat gggtgagtgg gatttccatg gatgatggaa gatgaagtga acttctaaaa
     1921 gccgaaaaac tcaagtatta tcttcaaaat actagtgctc aatgagatgc ttgatttgat
     1981 cacaagaaga ctgcgaaagc cgctggcaac cgcaagctag gtatcccacc gatggactac
     2041 gttttagagg ctactggtta tgaatacgga gccaacgctc tcatctgcat aagaatacat
     2101 cacccagatt atgtaaagat tttcgatact tctgtcaaaa aatcattaag atagataaat
     2161 gatttccgtc tagtcaaatt ggttcagaat gtagacacac agatggtaag aagtcactca
     2221 caattcgaga atggacttgt cctatttgcc gtatacatca taatcgaaac gtcaacgata
     2281 gtatcaatat tttgaccaag ggctatgaat acaagcattg ccttagaaaa catcaagaac
     2341 cttaggaaca acggggatag cttggttaat aagagatacc gctgtttagg cttgcaagta
     2401 tactctttta tcaagaattc cgtggcttta gtcatgagag gcttaatact atgaaaattt
     2461 tctttagcat ttttgattta ggttcctttt ctaattataa taagatgacc actacagtaa
     2521 cctactgaaa tatataaaca gccactgtag taccattttc aaagtagtct atgagaggag
     2581 aatcttcatt cttatgaacg ctggaattgc ggatatgaat ttaataaaaa aaacccttaa
     2641 tgattttact tccaattcaa tatcaaaagg tactggtatc aatttaagca caataaaaaa
     2701 gctgaaaagt ggtgaaagat cagtggaaaa gctgaatctt ttagatgcta taaaaataac
     2761 ggaatttgct atgaaaaatg gaaaagctga gattgaaata tggagataat tatcgtctag
     2821 tgaattactt gccacttagc tgaatttaac gatccttggt gcttgaaggg ggagattctt
     2881 gggaatagag catacttgca agcctatgtc tttactaaca acgatatctc ttattaacca
     2941 agctatcccc gtagttctaa ggcttttact tttatctaag caataatcgt attcaaagga
     3001 aatttttgac ccttagaaag tctcatttta tcagacgtga gcttaggcat tgctgaattt
     3061 tgttaattcg gttttaggtt gtgctttgag cataatatga aagtattctt tatcacgatt
     3121 tcattcatct aaggtaatgt gatgtgattc tgaaattctt tcaaaggcag tttttgcata
     3181 agctgacatt acatcatcaa tgacatgttt acaatgtttc actactaaaa taagggggta
     3241 accaagaaga aaaactgaat gattatttgt atctaattcc atggtatatc aaccatttcc
     3301 tatatagact gattatgaca tggattaaaa taaaaaggca ctatgtctta attgtcggtt
     3361 ttcgagttac cgaatggcat tcatcaccca cctatagagg atgggcgact tctgcctgaa
     3421 ataagttaca ctacttataa cttctaataa aattagattg agtcattatt atttaacatc
     3481 aacgattttc cagtttccat ttttttgtaa tactaatttg tactgaaagt agctagtggt
     3541 ttttgataca ttatcaaggt attttatctt tgtactcact agaaaatttt ttccgttttg
     3601 tagataaact ggattctcta actccgaaaa ctttaagtca atattgattg gtcgcatcgc
     3661 accattttca acataatact ccaattcttt ttcagaagct gatggataaa gtttaaaaaa
     3721 tgtttctaaa aaatcggtga tttcttccgt tgttttggca tcaatttctg aatcgttttc
     3781 tacttgtttt gcttcatatt ttgccttgtc tggctttcca atcattgttg gcaaactagt
     3841 tactaaatga ttcctgtttt catcttcaaa gattgtcact cgatatgcag aagaaattga
     3901 ttgcgtcttt tttccctctg taatatgttg ctccactaaa aatgaaacga caaattcttc
     3961 cgagtgtttt tcaacatcta gaatttttac agattgaact tcggaactcg tgggaatatc
     4021 tgctcgaacc atatcttgga ttaaagcgag accgttttct gtaaggtact gttcgagatt
     4081 actcattctc ttttcaattg cttctttgtt atttttccat gaaaaatatt ctttcgcaaa
     4141 atcctctgta aaactttcaa cgccagaagt attcactacc ttctctttta taaccacttt
     4201 ttcatgagtt gtatgttgat caatggcggt gaaattttta taaacgccaa aagcaagact
     4261 acaagacaat aaaagccaaa gggcaagcac catttttcga tgcttaccca cggatacaac
     4321 tcgttctttt ttcttttttg ggctcttttc cttttgattc cgttcaattt ttatcttcat
     4381 tctttttcct cttttctctt atttgtatcg gccagctccg ataagatgct gccgccaata
     4441 gttttccgtt agattcgtcc accccactgg atctcctgca tgaaacatgc gattctcccc
     4501 tgcgtaaatg cccacatggg tcacataagt accggcatca taggttgaat gaaagaaaac
     4561 taaatcacct ggtttggcat cttttagatc aatatgagtc atggcatcat attgatcctg
     4621 agccacccga ggaagtttca ggtcaacttt ggcataacac cattgggtca agccactaca
     4681 atcaaacccc gtttctggac ttgagccacc aaacacatag gccgttcctt catacttaaa
     4741 tgcctcatca aaaatttctt ggactatttt gtcatcaaac tgagttgtag ttaggtattg
     4801 cttcactaat aaacagtaaa acatattgcc ataattatac cgccacccac cattttcctt
     4861 aatcgcgatt gggttggggt aatcgacttt ttgaccacct gatttttcct ttgaaaaaga
     4921 ttcagctaaa gcaaaactat gcttcttccc gtttttcccg acatgatcta gatacccgcc
     4981 accatagtta taggcctgaa taatactatc ttgatcacac cctagttctt tggaacgctt
     5041 aaccaaagac gcaaaataga gacagccttg tttgatggat gcctcggtac ttaatgaatt
     5101 aggtggtaat ccggctgact cagaactttg cataacgtct tccattgtgc caccagattc
     5161 cacttgaata atcgccaaaa gaattggaat atactctgtt atatccgatt ctttggcata
     5221 cttttccacc attgcacggt gtttcaatac ttcttctgag acactagttc cgccaggaga
     5281 agtgccagaa ctaccatttc cctcactgtc ttcaccaaac aacaaaccca cacacatcaa
     5341 aatacccata aaagagatgg tcagaaaagt taatgaaata acacccactt tcttcaagtt
     5401 cattttttct cacctgacct tgattttgga cgatggttca ttcttttttg tactttttga
     5461 ttggtctgct ttggcagagt ggacctctgt ttcacttgtt ggaaagaatt tttcttactt
     5521 gctgattggc ttttcccttt tgaaatgatt tcttgactag ttagttttcg tttgatttcg
     5581 ggattcagat ttgctctcac aatccgcttt ggtgcttctt ttttcagcat catatctttc
     5641 tgaggttgag ctgctttatc tattggaata ttcgcccttc tttttccctg acgatttgtt
     5701 gcttcactca gtatcttgcg tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt
     5761 tctcgcaaat ccactcgatc cgctttttct tggaacagtc cgcgcttgaa ttcttctggt
     5821 gcttttttcg acttttcgat tcctttatgg agattgtatt tcaaattcgt tggcgtattc
     5881 cttacctgat cctttacttg tttagtctta tctttcacca aagcttgggt atccaaaact
     5941 ttgccgattt ttctcccagc caaattcatt cgggattgtt tcttattatt tgaaactgga
     6001 ttctccttag ttaagtcaga tagtatttcg tgatcgttcg gtaatcgtga attctttcgt
     6061 agagaagaac cggatggctt aaatttggat tttgattgtt cctttgccaa cttatttccg
     6121 actgctgcac caccaccaaa agccaaagct cgtcccacat ttcgattgag ttgccgcaat
     6181 tttcgattca ttaacatttg cggttttcgc atgacgcgtc ttcccattga ctggctatca
     6241 ttactctgga ggttgaacat actcataatg tcgcccaatt tgaaatagat accggcaaag
     6301 gtcacaattt gcagaaaagc aaccatgaaa aatggatagg ttgccgaaat attgaaaaac
     6361 atcgtggaga tgctaaatgc agtcgtaatc accaaagtaa cacccgctcg catcataatc
     6421 gtattgaata aacgaataat cgccttcttt cctaaacttt cataagtcgg caacatggac
     6481 aataagaaac taattggcag aaacatcgca aagataatga agagaatttg cgagaacaac
     6541 ataatccctg ttagaagaaa aacaaagaag gaaatgccaa tattgaacaa taccaaaaag
     6601 accacggtcc ctaaacggtt catggtcttg gtaatactga gattcgcatt gtcattgtct
     6661 tcaatctctg ccttgaccgc ttcttcccga tctttgcctt tgttttcatc tggactcaca
     6721 gatagaatct tgttgacccg gtcttcacca atttcttcta tgttggaatc atcaaattgc
     6781 agtagtaacc aaggttgttg gacttgaatc gaaaacaaac tatcccgaat caaatccaca
     6841 ctatcttttc cttggctttc tgaattgggt acaacgatct cggttccaag ggtaagggcc
     6901 gcttcactaa catctgagct aaagtcattg atttttgtga tataggtagg cgcataagca
     6961 atgaacgaac ccgatagtag aaaaatcatc acaaaattaa tcacggctcg aactgctttc
     7021 gtggtttctc gttttaataa tccggtatac gccacataca cccccagagc taaaatcatc
     7081 aataatagaa acccaaaata aaatccggag gtctggagtc cattttcagt aataccagcc
     7141 aatgtttgga tattctttcc aatactttca gccgtatccg aaatgaaatc taatttgtaa
     7201 gcctcttgaa ccacataacc cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa
     7261 ttagtaatgg cataaagacc atacatcacg cttttcccta agccatcacc ccaattccaa
     7321 ggcaaccaat cccaagagct atccacaaag aaatctagtt gatagttgtt cagtgaatat
     7381 ttggaatata gatttcctgc ttcaactgta tcatcaacta gccctgtcgc ctcggctacc
     7441 gtaccaacta aactcaatag aactatcaga gtgagtatgc taataaaaac gattcccaca
     7501 atccgaaaaa gtttcttttt catcccctca ctccattctc tcttgaacag gtggacgggt
     7561 atcaaaggca tggaagagtt cttcaaagac tggatgtacc tgaaccacgc ctacccgacc
     7621 atacaaatcc tgcatcaagc attggccatt ttccaaatct cgtaggcgtt tttgattgct
     7681 ttcatcttct cgatccaagc caaagaattc caaggttttc ttgatttcat gaatatcggt
     7741 actgcgaaac gcaaacttca acccgatatt attcttcaag cgttcatcca gtaaatcatc
     7801 actattttgc gtgacaaagt aaaccccggc attcatagcc cgcccagcac gaacgagctt
     7861 attcgataat gttttccctt gcgctacttg aagaaaactc cacgcctcat ctaagtctac
     7921 aattttgaag atttctcgat tactatggat aaagtccaag gcaaaagtgg aaatgacgat
     7981 tagcatggaa acactaagca attccatggt ggtatattct tcaaaagtgg tttctgcatc
     8041 tggtaaaacc aaatccgcta cttgaatgat atttaactgc ctatctaaac taatcgactg
     8101 ggtaatcgaa ccatctgaga aaagcaaatg ggcaaaatca taatcggtaa aggactcgat
     8161 gtgatccgcg atattttccg caactggtga gccttctttt cgaagctcat taattacttg
     8221 taataatccc cgttgattgc tttgggtaac cgtccgaatg gccttacgca acactggaaa
     8281 cttctcccca tctcgactag aaattcctgt taaaaaggtc agaatatcaa ttgctagact
     8341 ttcagaatcc ttctttttct ccataatcac ataaggatca agtaatcctt gattttttgg
     8401 cttactagtt aagttgagaa tattgatttc atccgcaatt tctggcaagg tttctttcca
     8461 gccacc
//
LOCUS       DACEKG010000098         8426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEKG010000098 DACEKG010000000
VERSION     DACEKG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.102"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            472..2109
                     /locus_tag="IW664_002436"
     CDS             472..2109
                     /locus_tag="IW664_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0849767.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2254..2994)
                     /gene="truA"
                     /locus_tag="IW664_002437"
     CDS             complement(2254..2994)
                     /gene="truA"
                     /locus_tag="IW664_002437"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0849768.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3185..3820)
                     /locus_tag="IW664_002438"
     CDS             complement(3185..3820)
                     /locus_tag="IW664_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0849769.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3967..5208)
                     /gene="sstT"
                     /locus_tag="IW664_002439"
     CDS             complement(3967..5208)
                     /gene="sstT"
                     /locus_tag="IW664_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0849770.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5406..6402
                     /locus_tag="IW664_002440"
                     /pseudo
     CDS             5406..6402
                     /locus_tag="IW664_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6418..>8426
                     /locus_tag="IW664_002441"
     CDS             6418..>8426
                     /locus_tag="IW664_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0849771.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2786 a         1547 c         1293 g         2800 t
ORIGIN      
        1 aaactaatca gaatcgtaca aatatttaat aaccaaatat atagaacata acttaatatt
       61 aagttatttg aaattccata aaaaacgact gcaggaatta ttgtaagaaa aggataaaaa
      121 tagttcagtc catctccata attgttaaat gttgtatagt tgattggccc ttcaaaaata
      181 gaagataacc ctttgattcg tgaaagatga aaagataaat cacctgttgt ataaatttct
      241 tgagcatttc tcataaaatt tactgaatat aaagtactaa gtaagataag aactactaaa
      301 agaaatgctt ttttacttat tttcatagat ttgttgttct ccttttttaa ttctattttc
      361 agtatactag aattgtcttt ttttaataag ttttgcgtta aatttttcat agcactagta
      421 tgatatacta gaaaagaatc aatttttatt acttttaaat aggagaaaaa aatgaaaaaa
      481 aacaaaaacc aaataataga tatcattttt atgttatttc taagttttgc ttacgtcatc
      541 cctctattca tgtctatggg aatctatcat agtgtcaatc aggatactta ctttcattta
      601 tctcgaatca taggactaga taatgtatgg agttctccag taaacttcaa taacttcgat
      661 catcatggaa caatgatgaa tattttttat ccatggctta ctctttatcc agctttttta
      721 ttttataaaa tgatgggaaa tcttgtttta agttataata tctactattt ttttatcact
      781 tttttaacaa tggtcgtttc ttatttttct atgaaacaaa taaaaaacaa tcgttatatt
      841 tcgctgttat tttcgattat ttatacattt tccgcctatc gtgccattga catattccat
      901 cgtgcttcat tgggagaagc tgtcgcttta acatttttgc ctttgatact aatggggtgt
      961 tacgaaattt atattagaga ttaccaaaaa tggtactggt tgtctattgg catgacgcta
     1021 gtagtgtata ctcatttact ttctgtcgct atggtttctg tatttattgg aggaacgttg
     1081 tttctttctt tttatttttg ggaccaaaaa atcgctagac tcttaagtct tttaaaagct
     1141 accgtcctca cctttttttt aagtgcggga ttcttaattc cttttattca acaatctaga
     1201 gcacaagaat tgaaagtccc actgggaaaa gaattaagtg gtatggctcc tagtgatatg
     1261 ctgacacaca ttttaaataa taactataac aactatacta ttggattatt cttatttctc
     1321 ggattgattg gtgcttttat ttttataaaa aaattaactg ctgatgattt atttattttc
     1381 tttcttggtg tatttgttct tttttgttca accaatttat ttccttggca actttttaac
     1441 catacaccag ttaagagtct acaatttgtc tggcggttaa atggtttttc ttctttattt
     1501 atagcctata cgatgagtat agtgatctat tatatttttt ctactaaaaa taattcatgg
     1561 aaaataatgg tctttacgtt tcttgcacta atattgcatc tctcaggagt aagcaattct
     1621 atccatgcta acaaagattc attaacactt atagcacctg aagacgctgt tgctatagct
     1681 gaaaattaca atcatactga ttatgcaaac aaagagtcaa tctatcatcc tgatatgatt
     1741 aataatgatc aatatttact aggtaaccag gaaataaatc caacaactca ttttatgagt
     1801 aacaaactta caattgaatt agataattcg aataataaaa atactgtctt gacgacacct
     1861 atttatcgtt ataaagggca agtggcttct attaatggca aactagtaca aacaaaatta
     1921 tctaagtttg gcacaactga attaactatt cctccaggta taaataaagt cgttattact
     1981 tatcagtaca caaaactagc aattgcttca agatatttat ctatcgttac tttgatttta
     2041 tttttacttt atcgatttac tttttctaaa caaaaacaac cgagaagaga agtacaacat
     2101 tctcactaga tttttattta agaaagactt tttagctgta cacataaaca ggctaaaaag
     2161 tcttttttta tatttcttta atttgcttca tacattattt aacatcgata tttatactgt
     2221 gaaacatgcg tttcatccat tatcaaagta tttttatcga taataaactt catccaaaaa
     2281 cagaccttgt gcagggacgg tttctcctgc atgttgccgc actttgcttt caaatatttc
     2341 atcgattgtt tctatcggca tggcacctgt gccgatttct agtaatgtcc ccatgataat
     2401 ccgcaccatt ttatataaga atccgtctcc gacaaaagta aaatgaagca tttctccctt
     2461 tcgttcgata gataattcat caatcgtacg cactgtggat ttttttgatt ttttcaatgc
     2521 agaaaaacca atgaaatcat gtgtacccac taatttttcg caagctgcat tcagcttgtc
     2581 catatccaac tcttgaggga aataaaagct gtggtttcgc tcaaatgctg aaggaatcac
     2641 gttgttccaa acataatagc tgtatttttt gcccaccgca ttatatcttg aatggaagcg
     2701 ctcaggcatt tcttcgattc ttttgatgac aatatcccgc ggaaggtaac gattcataaa
     2761 gtcgatcatt tctgccagtc caatttctgt gtttgtttta aaatttgcta cttgtcctcg
     2821 cgcatgggtg ccagcatctg ttcttcctga tccaataatt tctatctctt catttgtcat
     2881 ctgatgcaat actttttcga tttttccttg aatggtttta tcgtcatctc caagtctttg
     2941 ccatcctgaa tatctttttc cgtcatattc gattgtcaac ttgatgtttc tcatatttta
     3001 gtggctcctc tgtcttccat ttggtcttct ctctaccttt ttaagattgt agcacaaaat
     3061 atagctcctt tcttctctaa aacagaagct atacaagaaa actgtggaaa tatatatttc
     3121 agaaaaacaa aaaacagcca ccggatgaag tatccgctga ctgttcaatt tgacggctca
     3181 agttttactg tttgagatgc aagatactca accaggcaat gggcacataa ttaaagaaaa
     3241 aactgattct ttggcaaaaa cctattttat tcagtaatgg ccaagaattt atagaaggaa
     3301 ttcttgccag tccataaatc gccgcaaaaa gcaaactgat cacagacagt ccaaggtata
     3361 agtggcttac gtccatttgc ccattttttc gatagatcaa gaaaagaaga actggaaaca
     3421 ataaaaaacc actgtaacct aaacctgatc cgatattatg gacccatgtc gagacattcc
     3481 acgaagattc atgggtattg atactgaaca aacctgtaaa gatacaatca cctataccat
     3541 agaacgcgat ggctgctgta actagggtag ctaacacacg agatgtactt acaaatgcag
     3601 catacaatgc aggcaatgac aggatgaaaa agatccctga aaccacagac caaataagaa
     3661 acgcttttct tacaggactg gaaacctctc caaatacact gatcacgctc ttcatttgat
     3721 ctaattcagg ataatacaat cctaatacgt acggagtcaa aaaatcactc agcacgccta
     3781 ataataaaaa gaaaaaacca aatttacgta aaaaatccat gatacgcctc caataacaat
     3841 tacctatgct tttaattgtt attataagcg actttatctt gatttaacag aaacctctat
     3901 ttacacgaac atctatataa ctcattaaaa actcgaatca ttttaaaaat actaaatatc
     3961 taacgttcaa cgttcactta atccatctaa acaatctttc aatttgattt tttccccact
     4021 tcttttacgc gcgcctagct ctcctattgc agtaaacaac agatcacttg acgaatttaa
     4081 ggcagtttcc attgaatcct ggataacacc gataataaat ccaataccga cgatttgcat
     4141 cgcgacatca ttagagatac caaacaaact acatgctaat ggaatcaata acagtgaacc
     4201 gcccgcaaca cctgaagctc cacaagcagc taaagcagac aaaatacata aaaggacttt
     4261 caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca gtcaacgtca tcaaggaaat
     4321 ggtaatcgcg gcaccgccca tattgatcgt agcacctaac gggatcgata tggaataaga
     4381 ctcttttgtc aatttcaatc tttcagccaa catcatgttt atcgggatat ttgcggcaga
     4441 acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa caaaagaaaa tcaatggata
     4501 gggattttct ttaataaaca aaaacgtgag gaatgggtac acaactaatg cgacaaacag
     4561 catcgtaccg atcaaaacaa gtaaaagctg cagatactct gccaatccag ctattccagt
     4621 cgtagcaatt gattgataaa ccaatcctaa aatccctatc ggagcaaatg caataatcac
     4681 ttgtacgact tgtgacaacg ctatagatat ctgatccacc aactcttttg tcgattctga
     4741 acgtgctcga aaagctagtc ccaacaatac agcccagaat aagacgccaa ggtaattcgc
     4801 atcaataaga gattgaacag gattttgtgt gacattcgtc aacatagtag ataaaacact
     4861 ttccaaactt tgcggtgctt tttcagatac tgtttcttgt aaagtaatag gaacagtaaa
     4921 aattgaactt acaataactg ctacaagtgc agaaagcaat gttcccacta aatataagat
     4981 caaaataggc ttcacgaatg tcttattccc aattttatgc ttggcaattg acgccatcgt
     5041 caagaaaaaa accagaatag gtgcgattgc cttcaagcaa ctgacaaaca acgttcccaa
     5101 tattgaaata aatgtccatg tggggaaaaa gataccaaac aatattccga ctaaaatacc
     5161 aacaacaata cgttgaacta acgacatatc caaaaacttc tctaacaaat gaccaccctt
     5221 tcacaatttt aacaatcatt gtatcatttt atgcaaagaa actaaaggta taattttcta
     5281 aaaattctaa caatacaaac aaccatttct tgctaatttt gtactttttc tgtctacacg
     5341 ttgttttttg agatattcta agttaggatg ttattaatgg gcaacttatc aggaaacgtt
     5401 ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt cttgaaaaaa ataaagaagg
     5461 agagttatgg cttcctgaca atctttgtcg cttgtataat gatggtttta cagcgtatat
     5521 tgatttcagc agtcactatt cctacatatg caacaaaccc tggtcgattc tacatctata
     5581 catttttaca aacttccgtt gttattggtg caaatttgat cccactttac atgggttacc
     5641 aatatcaaaa aatcgcccgt ttaaaagttt tacattattt aagtcgtttt gcctttatat
     5701 tcctaatagc cacactagtt tgcaatatct tttttttacg tacaagctgg cagcttgaat
     5761 attcgtgatt attggcttat cctaacacct atcagtcaga attactttac ttatgctgtt
     5821 tcttgtactt tgcttttttg tagtattcct tatacagttg gatttttaaa taaattatcg
     5881 aaaacaacaa ttaaatatct actattattt ttaacaattg gattagttgg ctgctctact
     5941 ctatttaata aagacccgtt tgcattacag aatggacaga gtattttatg gattttttat
     6001 ttatttttaa tgggttatgg tttgaaagaa tggaactgga aagaaaaggt ccgctataag
     6061 cttcctcaag tatttatcag cttgattgtt ttattcggtt tgattatttt gatgacacaa
     6121 atttcactca tcatcagagg aaatacagat acagcgctga ggttttctaa accatattct
     6181 ctcttcgcaa tgtattatac tttctcttcg tttttatttt tagaaatgct atctgataaa
     6241 ttaggtataa aaggaagagg aaattttctt gctgtatttt tgattgtcac tcaagttata
     6301 tgtaatttcc ctttagtttc ttatcgcgta tctacattta tgaaacgtga atttccaaac
     6361 tcagggaagg catggctgat aaatatcggg cagtttttta tttgctattt attagcagtg
     6421 tccctcctag ttatcttttt attttatata caaaaaatcc gtttcattca tcgtttcatc
     6481 gaatacttcg cttttgattc gattgaacaa ttaactcagc gttggaaaaa aagaattcat
     6541 tggctttttg ttcataaaag ttatttttta gttgcagcat ttttttactg ctttaccttc
     6601 gtgcagattt tcgttttaga accaaaagaa ggttggaaag aaactatcca ggttgcctta
     6661 aaaatattta ctcagcgaca agcacccatg atcctgacaa tcatcattat tatgggattt
     6721 ttcttcttac tattgttgat ttccaatcgt ttttggtacg ccttgactgc taccttgatc
     6781 attaacttac tgctcactat ttcgacagta atcaaaatgg aaatgcggga agagcctgtc
     6841 tttccttcag atttaaaaat actgaccgga ttgtcagaac tattatcgat ggttagccca
     6901 gtcttattaa tcgtgggcgg attgatcctt ctcctattat tgattactag tattattgta
     6961 caacgtagac tacaaaaaca gtattccttg aagattcatt ggaaacgtcg atttatcagt
     7021 atcgctgtat tattaggatg tttttcgggt gtctttttca ttaatcacaa aaattcgcct
     7081 tctttcttgc tgtttaattt atttaaagta aacaagactt tctttaacca gcaaggcgcc
     7141 gtaaaagata acggaccagt cattcaattc ctaaacaata tcgacattaa aatcatggaa
     7201 gaacctgctg gctattcgaa agaaaaaatt aatggaataa tgaaaaagta caacacaaag
     7261 gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca atcagacgat cgttctcaat
     7321 ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc tgacagtacc tacagaccct
     7381 attccttaca tcaaaagcat tataacagaa ggaacaggag gattgatgtt atccgtcggt
     7441 tatggtggcg gaacagctaa catggaatgg gaaggactga caggcttgag tatcagtaac
     7501 ctgtctgctt cgttagttac accttatacc cagctagtgg agcgtcagaa aataagtcca
     7561 aatattaccg atctattcga tgaaaaaata gccatccatc cttttacagc tgctctttat
     7621 cgcagaaaac aagtgtttga taaatttggt tttgacaggt tctattattt agacagtccg
     7681 gataaattaa cttatacgga taaaatagaa caaagcccac gcatatcgga tgattcagca
     7741 tacaaagaaa ccttgaaagc tttacaaagc aatacagaaa cgtcacaatt catccaatta
     7801 tccactatgc aaaaccatat gccttatgat gattactata gtgaattgaa ctacactgct
     7861 gaaggaactg ctgtcattcc taatagaaga aaagaattac aaacttatat gcaaggcctt
     7921 cactataccg atacagcagt aaaaaaattc atccaagaaa tcgacaaaat cgataaaccg
     7981 attacctttg ttttttatgg tgaccattta cccgcaatct atagtgggaa tgattcgaaa
     8041 aaatatggat tagaacagca ccagacagat tatttcattt ataacaacaa gtatagtcgt
     8101 gaacaagcag ctacgttaaa taaagaagtt gttgctccta ataactttgg tgctatggct
     8161 cttgagcatg cgaatatcaa agtaactcct tactatgcgt tgttgacaga agtatctaat
     8221 catctacctg cattaacaat tgatcctaca gaaagcttgt ctaatcgttt taatggaaaa
     8281 caggtctttg ttacagagga tagtcaaatt atccaagaaa aaaatttaac tgagaaacaa
     8341 aaagaaattc tagaagatta tcgtctgata caatatgatc ttgttgcagg taaccagtac
     8401 agtgcaacat gggcgacaca aaaaat
//
LOCUS       DACEKG010000099         8400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEKG010000099 DACEKG010000000
VERSION     DACEKG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.103"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(400..717)
                     /locus_tag="IW664_002442"
     CDS             complement(400..717)
                     /locus_tag="IW664_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0849772.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(718..972)
                     /locus_tag="IW664_002443"
     CDS             complement(718..972)
                     /locus_tag="IW664_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0849773.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1331..1735
                     /locus_tag="IW664_002444"
     CDS             1331..1735
                     /locus_tag="IW664_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0849774.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1752..2900
                     /gene="tnpB"
                     /locus_tag="IW664_002445"
     CDS             1752..2900
                     /gene="tnpB"
                     /locus_tag="IW664_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0849775.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3123..3341)
                     /locus_tag="IW664_002446"
                     /pseudo
     CDS             complement(3123..3341)
                     /locus_tag="IW664_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3607..3801)
                     /locus_tag="IW664_002447"
     CDS             complement(3607..3801)
                     /locus_tag="IW664_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849776.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            5153..6193
                     /locus_tag="IW664_002448"
     CDS             5153..6193
                     /locus_tag="IW664_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0849777.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            7017..7349
                     /locus_tag="IW664_002449"
     CDS             7017..7349
                     /locus_tag="IW664_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0849778.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            7968..8309
                     /locus_tag="IW664_002450"
     CDS             7968..8309
                     /locus_tag="IW664_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0849779.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         2977 a         1266 c         1417 g         2740 t
ORIGIN      
        1 catagatata ttggacttcc tcttttaaaa cacaaaaata aatactacct tatttaggta
       61 gtatttatta aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag
      121 taaagtcacg aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc
      181 agtgtctctt attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga
      241 ctaatgattg tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag
      301 tcttatatat caatcttcga gtaaccgacg gttaacaacc atatcgagcg gcattaatca
      361 cccacctgta gaggatgagc gactttttcc taattattgt taaaagccta gtttcatttg
      421 tttagattgg aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa
      481 taaggcatag cttttgccga tcacgatccg atatgttggg atatccattc catagatttc
      541 ggaaacttcc tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc
      601 acctacaaat ctttttatgc tggctggaga aagagcaagc tcattttccc attcttctat
      661 tatcgtttca aggctggtct gaaaactttt agaataaact agcgtgtact ctttcatcta
      721 ttcactccag ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc
      781 tttctcaaag tcttggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg
      841 agtagtatct aatatgaata agttgtcttc ttgtttggct tggataatgt ctccgctttt
      901 taatccaaga gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag
      961 ttcttttccc atgttatcac actcctttta gtttttgatg aataaattat aacatgttat
     1021 aacaacaatt gccaacggtg tttcttaagg gattccactc catattatta ttggacaaaa
     1081 taagccactt agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc
     1141 accatacact tccgttaaca tccatttaac agaattaggc agaagtcgcc catcctctat
     1201 aggtgggtga tgaatgccgt tcggtatgag ggttaccgtt ggtaactcga agaccgacaa
     1261 ctaaggcgta ttgccttttt attttgtccc atgttacaat cagtctatac aagaaatgag
     1321 ttgatataac atggaattag atacaaataa tcattcagta tttcttcttt attaccacct
     1381 tgttttagta acgaaatatc gtagacaagt gattgatgat gaaatatctg attatgccaa
     1441 aactactttt gaaagaattt cagaatcgta tcacattact ttagtggagt ggaatcatga
     1501 taaagaccac gttcatatta tgttcaaagc tcaacctaaa acagaattga caaaattcat
     1561 caatgcctat aaaagtgcca gctcaagatt gataaaacgt gactttccaa gagtcaaaca
     1621 atttctttgg aaagaaatgt tctggtctaa aagcttttgt cttctgacaa ctggtggcgc
     1681 accaattgat gtgattaaga agtatatcca aaatcaagga aacaatcata aatagaaagt
     1741 aggtgaactt tatggaacaa ctaaaagcat acaaattcag aatctatcca acagaggaac
     1801 aagaaatctt ctttgccaaa tcttttggtt gtgtccgtaa ggtctacaat ctaatgcttg
     1861 atgaccgaaa aaaggcgtat gaagaagtta aaaatgattc ttctaaaaaa atgacttttc
     1921 cgacaccagc taaatacaag aaagaatttc cgtttttgaa agaaattgat agccttgctt
     1981 tagccaatgc ccaacttaat ttagataaag catacaagaa tttttttcgg gataaatccg
     2041 ttggatttcc acgtttcaaa agtaagaaaa atcctgttca gagttataca acgaataatc
     2101 aaaatggtac aattgctttg attgatagta aattcatcaa agttcctaaa ttgaagtcat
     2161 tagtaagaat taagcttcat agacaaccaa aagggattat caaatctgct acaatatcac
     2221 gtcattctag cggtaaatac tatatttctt tgttgtgtaa agaagaaatt atcgaattac
     2281 ctaaaactaa ttccacaatt ggtattgact taggtattat ggactttgcg attctttctg
     2341 atggacaaaa aattgataat aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg
     2401 aacaacgcaa attgtctagg cgtgctttat tggctaaaaa taaaggtacc aatctatttg
     2461 aagctaaaaa ttatcaaaaa caaaaacgca aagtagctag attgcatgaa aaagtaatga
     2521 atcaacgtac tgattttctg aataagttga gtacagaaat gatcaaaaat cacgatatta
     2581 tctgtattga agacttaaac acaaaaggta tgttgcgaaa tcataaatta gctaaaagta
     2641 tttctgatgt ttcttggtca aagtttgtga ctaaattaca atacaaggct gactggtatg
     2701 gacgaaaaat catcaaagta gataaatggt ttccgtctag tcagatttgt tcagagtgcg
     2761 gacataaaga tggcaagaaa tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata
     2821 ctcatcatga tcgtgatatc aatgctagta tcaatattct gaccgaaggt ctaagactac
     2881 attctatggg attggcttag atccaatcat caagaaccgt aggaactacg gggatagctt
     2941 ggtaaataag agacaccgct gttagtaaag aaatacgcta tcaagtatgc tctattccca
     3001 agaagctccc acttcaagcg ttaagaaccg tcaggtttag ctaagtggtg agtagttcac
     3061 gtaggtattt ttattatata tagatttaac atatgtcggt cggttgattg atatagctaa
     3121 agttataact caaccgaagc cgattcatct acacgaataa agttgcgagt gttctcggct
     3181 aattcataaa aattgttatg tgttccatat agcaatactg gaataacttt attttcttga
     3241 atctcataag caactctata cacggtgacc gcatacttaa atccattaga ccaattacct
     3301 gataaacctc cagttttttg ctcttactta aatggatcaa tggcaatttc atcacaaacc
     3361 tatagaggat gagtgacttc tgcctaatta cgttaaaaaa tattgaaagt aactctatgt
     3421 agcaaaaaat atctagaatt tcatttcctc aaaactgatc gatattgtat ctaaaaatat
     3481 atactaccta aaataggtag tatatattaa aatatgtgct tttgttagaa aaatcaatac
     3541 ataaattatc atatctgtat atgtcattta ggtatttcag ttatgttata atagttttgc
     3601 tgtctgctac cccggcctaa tctggcaagg ggaggaggag atataatgaa ataccttttt
     3661 gattttgttt ttgcaatcaa gccacttgca gatgtagtgt gcagttacct ctgcaaatgg
     3721 cttgattgca aaattgccaa aaaagacagt aagcctaccc gttaattcta taatatagaa
     3781 acgtaaaaaa aaccccatca gcctgcacgc tgatggggtt tggtgatata atgaaatacc
     3841 tctttgttac tcataattgt accataatta gtcacgaatg caaccaatag aaaaaactac
     3901 ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat tttgtgaagc
     3961 attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc ttctattata
     4021 aaagaagata tatatcattt cttaattacc tactacctta aaccaaaatt taagtactac
     4081 ctaatttaag tattacttaa acaatctaaa aaatagttat cccaattgtt atgcttttat
     4141 aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat atttaggtag
     4201 tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc aactaagtta
     4261 tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact acaagcatgt
     4321 agttttatct gttaattatg tactacctaa ataaagtagt ttataattag caagtatata
     4381 tatttaaaaa tttaagatta atttttttat gacttgtaca aatttatctg atattctctt
     4441 attaagaact acctaatttg ggtagttctt attttttaca ttttaagtag tatttaattg
     4501 caatttggta gtattttttt attatcttat tcttagaacg gtagtactta atataaatat
     4561 aggtagtatc taatttaagt attaatggta gtatctaaat taacaggtag tatgtaacta
     4621 ctttaaagta atttttttca gattaacaca gtagtatctt attaaataag ttaattttaa
     4681 tggtagtaca taattattat atatatctga cattggtagt acttattgta ttttttagta
     4741 gtatataatc tattgttttc tagcctaaag ccttgtctta taagaataat ataatatata
     4801 ctactgaaat aaggtagtat atattactag taatggtagt atgttttata tgcataaggt
     4861 agttcttaat ttattttttg ttttgacact ggtagtactt aattatcttt aattttaaaa
     4921 ctaaatttta ttcatacgtt tttctggtag tatttaatta taaataatat ttatttaaag
     4981 tagttcttaa tgactttaaa ggtagtacat aattcatttt atttctgatt ttaggtagta
     5041 tttaattaaa aaacagtttt aatgcgtttg cattttttat ttatctttgc tattttcgaa
     5101 aagagtaggt aattttacat aaataaaaca acgtgaaagt agaggatcta caatgactga
     5161 ttttaaattt tttaaggcgg atagagttta caacgaatta ttttatcaat ttccaaaagt
     5221 ctttattgtt tctgacgaat acaaaaaaat gaaagattca actaagattg cctatatgct
     5281 tttaaaagca agattagaga tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt
     5341 ttattttact tatacgacaa atgaactatg tagagtatta aactgccaaa aacaaaaagc
     5401 gatagcaatc aaaaaagagt tggaatcctt tggtttatta ttacaaaagc agatgggatt
     5461 taacaaacag ttagggaaaa ataatcctaa tagactatat ctagcagaat taaaagtctc
     5521 agaaaatgat atctacttac tcgaaaaatt tgatagagag aatagggaaa acgttgataa
     5581 atcagagggt atgaaaatca tacccaccct cgacgaaaaa tcagacgctg aatcccttgg
     5641 ggctcaagag ggtatgaaaa tcataccgtg ccaaaacgtt gataaatcag agggtatgaa
     5701 aatcatacca gaacttaata ataatatatt agacactaat agacacaata tagacactga
     5761 aaaagaccgc ctacaagatc aattgttgtt agacaatttt gagacaatta tgacaaacga
     5821 cagcattgct acgtttgtcc ctgaacgagt attaaatttg ataaaaacat tttcttcaag
     5881 ttacagtgaa gctcaaaaaa ccgtccagac tattcataat gcaaagaaaa aagctgaaat
     5941 agaaagtggt atttcgatag tttttgaaga actcgatagt tattatgtca atgcagaaca
     6001 agaattatac acgacactgt taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa
     6061 tatccagaac ctgatttttg tctatgtaaa aaattggttt atcgaaaaac caatagctgc
     6121 taaagtatca agtgaaaaac gtttgaatta tgaaagctcc ccaagcacta ttacgaaaga
     6181 ctggttagag tgaaacttta ctgacaaagt gattgttgct tcatggagta acattaaaat
     6241 taaaaatttt ttattattct aattatatta cgaatattac aaaataatag ctattttaat
     6301 agaatttata agttaaaata ttatgataaa aatcacaaac gtagcctttt acgtcataag
     6361 tgttaaaaga aataattaat ttcaaatata gaaaagggaa cgtatgtttg catactcctt
     6421 ttttttgagt tataataata cgcgaagaga gataatttca ttatttaaac aagctaaaaa
     6481 tcttgatatt acttcttttc aggcaatacg aatattattc gcttacattt tttgtataaa
     6541 tttaattgtc tgctatgctt gtgatgaatt gattttcgtt ctgcactttt ggatgataaa
     6601 tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag gctggtaacc
     6661 ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac ctttcagtgt
     6721 tacggtatta cccgcaatgg atttagtata acagatattc aaaagtgata ccactttttt
     6781 tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta agcacaatgt
     6841 tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag agggaacgca
     6901 tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc tctatttttg
     6961 aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt tattttatgt
     7021 catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta
     7081 cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga
     7141 aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt gggaaacaaa
     7201 agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca atcaaacatg
     7261 tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg
     7321 caaaagggat ggggcgtttg atcggctgaa ttgatcattc tcataacttg tattttatcg
     7381 tttttcgctt gcttgaaaat attgtcgaga cttctaaagg aacttagagt cactagaaaa
     7441 ataaggagta gacttgatct atcaacaatt cccttcgggg agtgacgggg taggttttaa
     7501 cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa
     7561 atgaatttgt gtaaataata agcgcagagt aaacaagagg gaatttagaa gttagacaag
     7621 ttagaatagc gtctagatta atcatgagca gctactaagg gatctagact ttgacgcaaa
     7681 ctcatggaat aaggagtagg tcttctcgta atggggagga cgcacaagaa attgtgtcgt
     7741 gtgctgcagt acatgtagat acttgcgagg acgttgatta ttggatcaat cgagcattta
     7801 ccgctgtcta ctagggtttc tcgtttcgga cgccataaac gagtcgaaat tttttatgta
     7861 tttttaagta aacgaaaaac gataaaatat gaacaagtcc tgaataaagt gaaaaaattg
     7921 atataaaaca tagagaaaat gaaatttacg taatggagga atgacaaatg aaaaattatg
     7981 atccgaatat acgattgggc acacatacta ttaaggtttc tttccaacgt tgggattaca
     8041 aaggatttgt gacgtttaga agaggcggaa attgtaaagg attagatgtt ttagcattag
     8101 acgaggatga tttatacgat caaacattga ctgataatcc aattggattc ggcttgctac
     8161 ctgaagatga tgaaggtaat gaatggttta aaatgacgct tatgaatgat aatggagacg
     8221 aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc tttgaaatca
     8281 ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta cagataaagg
     8341 cttagaagga gtcacgatga ttaataaaaa ggtagtatta ccttaggcat tgagtaagga
//
LOCUS       DACEKG010000100         8287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEKG010000100 DACEKG010000000
VERSION     DACEKG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.104"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            213..1406
                     /locus_tag="IW664_002451"
     CDS             213..1406
                     /locus_tag="IW664_002451"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0849780.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1536..3008
                     /locus_tag="IW664_002452"
     CDS             1536..3008
                     /locus_tag="IW664_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0849781.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3091..3879
                     /locus_tag="IW664_002453"
     CDS             3091..3879
                     /locus_tag="IW664_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0849782.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3946..4614)
                     /locus_tag="IW664_002454"
     CDS             complement(3946..4614)
                     /locus_tag="IW664_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0849783.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4729..6441
                     /locus_tag="IW664_002455"
     CDS             4729..6441
                     /locus_tag="IW664_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849784.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6444..8216
                     /locus_tag="IW664_002456"
     CDS             6444..8216
                     /locus_tag="IW664_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849785.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2539 a         1387 c         1822 g         2539 t
ORIGIN      
        1 aaaagcttag cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa
       61 tgtaaaatat ttttatattt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
      121 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
      181 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
      241 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
      301 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
      361 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
      421 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
      481 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
      541 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
      601 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
      661 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
      721 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
      781 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
      841 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
      901 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
      961 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     1021 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     1081 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     1141 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     1201 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     1261 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     1321 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     1381 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     1441 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     1501 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     1561 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     1621 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     1681 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     1741 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     1801 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     1861 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     1921 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     1981 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     2041 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     2101 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     2161 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     2221 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     2281 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     2341 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     2401 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     2461 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     2521 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     2581 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     2641 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     2701 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     2761 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     2821 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     2881 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     2941 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     3001 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     3061 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     3121 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     3181 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     3241 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     3301 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     3361 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     3421 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     3481 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     3541 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     3601 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     3661 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     3721 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     3781 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     3841 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     3901 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     3961 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     4021 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     4081 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     4141 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     4201 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     4261 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     4321 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     4381 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     4441 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     4501 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     4561 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     4621 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     4681 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     4741 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     4801 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     4861 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     4921 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     4981 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     5041 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     5101 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     5161 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     5221 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     5281 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     5341 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     5401 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     5461 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     5521 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     5581 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     5641 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     5701 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     5761 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     5821 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     5881 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     5941 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     6001 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     6061 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     6121 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     6181 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     6241 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     6301 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     6361 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     6421 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     6481 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     6541 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     6601 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     6661 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     6721 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     6781 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     6841 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     6901 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     6961 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     7021 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     7081 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     7141 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     7201 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     7261 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     7321 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     7381 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     7441 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     7501 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     7561 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     7621 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     7681 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     7741 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     7801 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     7861 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     7921 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     7981 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     8041 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     8101 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     8161 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     8221 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     8281 ggcagaa
//
LOCUS       DACEKG010000101         7624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEKG010000101 DACEKG010000000
VERSION     DACEKG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.106"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(15..707)
                     /locus_tag="IW664_002457"
     CDS             complement(15..707)
                     /locus_tag="IW664_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0849786.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            792..968
                     /locus_tag="IW664_002458"
                     /pseudo
     CDS             792..968
                     /locus_tag="IW664_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="IW664_002459"
     CDS             complement(1144..1713)
                     /locus_tag="IW664_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0849787.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="IW664_002460"
     CDS             complement(1787..2110)
                     /locus_tag="IW664_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849788.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="IW664_002461"
     CDS             complement(2264..3076)
                     /locus_tag="IW664_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849789.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="IW664_002462"
     CDS             3430..4278
                     /locus_tag="IW664_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0849790.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="IW664_002463"
     CDS             4423..5364
                     /locus_tag="IW664_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0849791.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="IW664_002464"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="IW664_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1372 g         2469 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacctatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caacgtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtgtgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg taggatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcggcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttc gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 acttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gttacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgagaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatga atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagctttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaacg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctcgaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caac
//
LOCUS       DACEKG010000102         7599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEKG010000102 DACEKG010000000
VERSION     DACEKG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.107"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..427)
                     /locus_tag="IW664_002465"
     CDS             complement(137..427)
                     /locus_tag="IW664_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849792.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IW664_002466"
     CDS             complement(545..805)
                     /locus_tag="IW664_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849793.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IW664_002467"
     CDS             complement(942..1355)
                     /locus_tag="IW664_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0849794.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IW664_002468"
     CDS             complement(1811..2296)
                     /locus_tag="IW664_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849795.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2680)
                     /locus_tag="IW664_002469"
     CDS             complement(2435..2680)
                     /locus_tag="IW664_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0849796.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IW664_002470"
     CDS             2873..3673
                     /locus_tag="IW664_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0849797.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IW664_002471"
     CDS             3692..5560
                     /locus_tag="IW664_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0849798.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFDLIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IW664_002472"
     CDS             5553..6044
                     /locus_tag="IW664_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0849799.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IW664_002473"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IW664_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0849800.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="IW664_002474"
     CDS             6604..7599
                     /locus_tag="IW664_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0849801.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1215 c         1442 g         2235 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga agctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 cctcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg ttcccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DACEKG010000103         7402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEKG010000103 DACEKG010000000
VERSION     DACEKG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.109"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            69..152
                     /locus_tag="IW664_002475"
     CDS             69..152
                     /locus_tag="IW664_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0849802.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="IW664_002476"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="IW664_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0849803.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1184..1444
                     /locus_tag="IW664_002477"
                     /pseudo
     CDS             1184..1444
                     /locus_tag="IW664_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1607..2272
                     /locus_tag="IW664_002478"
                     /pseudo
     CDS             1607..2272
                     /locus_tag="IW664_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2308..2553
                     /locus_tag="IW664_002479"
     CDS             2308..2553
                     /locus_tag="IW664_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0849804.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2656..3525
                     /locus_tag="IW664_002480"
     CDS             2656..3525
                     /locus_tag="IW664_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0849805.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3506..4240
                     /locus_tag="IW664_002481"
     CDS             3506..4240
                     /locus_tag="IW664_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0849806.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4273..5185
                     /gene="ant(6)"
                     /locus_tag="IW664_002482"
                     /pseudo
     CDS             4273..5185
                     /gene="ant(6)"
                     /locus_tag="IW664_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5182..5724
                     /gene="sat4"
                     /locus_tag="IW664_002483"
     CDS             5182..5724
                     /gene="sat4"
                     /locus_tag="IW664_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0849807.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5817..6611
                     /locus_tag="IW664_002484"
                     /note="aph(3')-IIIa"
     CDS             5817..6611
                     /locus_tag="IW664_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0849808.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6719..6907)
                     /locus_tag="IW664_002485"
                     /pseudo
     CDS             complement(6719..6907)
                     /locus_tag="IW664_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6887..>7402
                     /locus_tag="IW664_002486"
     CDS             6887..>7402
                     /locus_tag="IW664_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0849809.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         2593 a         1119 c         1556 g         2134 t
ORIGIN      
        1 ttgtgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttaca gatgtaataa cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
     1081 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1141 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1201 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1261 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1321 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1381 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1441 ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta
     1501 gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact
     1561 ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta
     1621 taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt
     1681 tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta
     1741 tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc
     1801 ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg
     1861 gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt
     1921 tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt
     1981 tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga
     2041 tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga
     2101 tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat
     2161 agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc aatctttgga
     2221 agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag aagggggctg
     2281 acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt ggaagaagct
     2341 attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga tttattcaag
     2401 ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac gttattttta
     2461 aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc atctaatcag
     2521 cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt aaaaaaatta
     2581 taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag tgaatcgata
     2641 aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa attatcctct
     2701 ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac ccatacagag
     2761 gattcggata tagatatcgg catctattac aattcagaat catttgacat aaatactatt
     2821 aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt acctcccgga
     2881 gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta tcatgtggat
     2941 ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga gcacggaatt
     3001 gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat gtatcgagga
     3061 gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga attaaaaaaa
     3121 caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt tatgtttgaa
     3181 gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa atattatatt
     3241 gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc atgtaataat
     3301 gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga acataaacct
     3361 gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc actttttgaa
     3421 tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc ggagataaat
     3481 aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt
     3541 tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga
     3601 gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg
     3661 ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga
     3721 tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata
     3781 tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg taatactaag
     3841 ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat
     3901 gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg
     3961 aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta
     4021 ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag
     4081 aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt
     4141 ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg
     4201 cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg
     4261 aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa
     4321 caggatgaac gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa atattaatat
     4381 acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata tagaaccgtt
     4441 tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc aaaagccgga
     4501 ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc tatttgatga
     4561 ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt acctaaaggg
     4621 cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg acatagttcc
     4681 gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg attgctgcaa
     4741 tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg aaattttatt
     4801 tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga tatcatggaa
     4861 ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt ttattgaaag
     4921 gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg attcctatga
     4981 aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat ccggtgaggt
     5041 ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa aatataccag
     5101 ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat atgccgctga
     5161 tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct gaaagatatc
     5221 gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta tgaaaacgag
     5281 aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta ccaagacgaa
     5341 gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac tgataagata
     5401 atatatcttt actaccaaga cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg
     5461 aaccggtacg cttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata
     5521 ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta
     5581 atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa ttgtggtttc
     5641 aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct
     5701 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     5761 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     5821 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     5881 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     5941 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     6001 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     6061 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     6121 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     6181 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     6241 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     6301 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     6361 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     6421 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     6481 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     6541 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     6601 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     6661 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     6721 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     6781 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     6841 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     6901 cagagtaacc tctattctta ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat
     6961 tgcacagcga tttaatataa gcttaaggac agtttacaga gatatccgga cacttgaaga
     7021 agccggaata ccaattggat ctgaggccgg aaaaggatat tttcttgtag agggcttcct
     7081 acttccgccg gtaatgttta cagcggcgga agtgggtgca ttgattacag cagggaaatt
     7141 tttaaattgc catggagatg aatcatttat aaaggatttt gattcagcta tgtataaaat
     7201 caaatctatt ttgaagcatg gtgaaaaaaa ctatgcacag gagttggaga acagtattaa
     7261 cgtgtacagc acatctggac agaaaaatac attggcggat aacgttattg cagcaattca
     7321 gactgcaatc tgcaataaaa gggtaatatc aattcaatat cctgcatcag gagggcaaga
     7381 accggaaagc agaatgatag ag
//
LOCUS       DACEKG010000104         7187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEKG010000104 DACEKG010000000
VERSION     DACEKG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.110"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW664_002487"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW664_002487"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0849810.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IW664_002488"
     CDS             complement(1172..1867)
                     /locus_tag="IW664_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0849811.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IW664_002489"
     CDS             complement(1880..2596)
                     /locus_tag="IW664_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0849812.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IW664_002490"
     CDS             2926..3534
                     /locus_tag="IW664_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0849813.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IW664_002491"
     CDS             3859..4182
                     /locus_tag="IW664_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0849814.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IW664_002492"
     CDS             4364..5734
                     /locus_tag="IW664_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0849815.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IW664_002493"
     CDS             6081..>7187
                     /locus_tag="IW664_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0849816.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACEKG010000105         6974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEKG010000105 DACEKG010000000
VERSION     DACEKG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.111"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(362..1480)
                     /locus_tag="IW664_002494"
     CDS             complement(362..1480)
                     /locus_tag="IW664_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849817.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="IW664_002495"
     CDS             1951..3258
                     /locus_tag="IW664_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0849818.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="IW664_002496"
     CDS             3377..4576
                     /locus_tag="IW664_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0849819.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="IW664_002497"
     CDS             complement(4603..5871)
                     /locus_tag="IW664_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0849820.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6034..6680)
                     /locus_tag="IW664_002498"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="IW664_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         2113 a         1457 c         1241 g         2163 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 ggcatctgcg gtaa
//
LOCUS       DACEKG010000106         6906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEKG010000106 DACEKG010000000
VERSION     DACEKG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.112"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            706..2565
                     /gene="ltrA"
                     /locus_tag="IW664_002499"
     CDS             706..2565
                     /gene="ltrA"
                     /locus_tag="IW664_002499"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0849821.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            2659..2898
                     /locus_tag="IW664_002500"
     CDS             2659..2898
                     /locus_tag="IW664_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0849822.1"
                     /translation="MTSKDYLSLFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRG
                     YVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            2976..3650
                     /locus_tag="IW664_002501"
     CDS             2976..3650
                     /locus_tag="IW664_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0849823.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            3883..4317
                     /gene="dcm"
                     /locus_tag="IW664_002502"
     CDS             3883..4317
                     /gene="dcm"
                     /locus_tag="IW664_002502"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0849824.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            4318..5025
                     /locus_tag="IW664_002503"
     CDS             4318..5025
                     /locus_tag="IW664_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0849825.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            5015..5305
                     /locus_tag="IW664_002504"
     CDS             5015..5305
                     /locus_tag="IW664_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849826.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            5564..6748
                     /locus_tag="IW664_002505"
     CDS             5564..6748
                     /locus_tag="IW664_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0849827.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            6763..6882
                     /locus_tag="IW664_002506"
     CDS             6763..6882
                     /locus_tag="IW664_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0849828.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         2401 a         1121 c         1493 g         1891 t
ORIGIN      
        1 ttaggcactt aaaagaagaa aacataagtt tccttttaag gagaactgat attttgagca
       61 gtggaatgat ggggtaacgc cctgaaacgc tcccttaata ctccgactgg ctgtttaaca
      121 tatagctgtt agatggtcag aagctcggtg aagtcggctg aaagttaccg taaatatgat
      181 aaattcatat acgaaagttg ttcagaggtg aatgatgtaa cctataggtc ggaggtctat
      241 aaaatactca cggtgagaat gtaagtaata ctactgacga acttgcgaat gaaagaatct
      301 atacacggac tgtatagaaa tgtacaggat tctaccgtat caacggaaca gaatgtatgt
      361 tgtggtttag taagattatt ggttatgaac aaccatgcaa tgttacaggc attggaaata
      421 tacaggttca aagcaagcac ctaaaagtat atataaagat aaaaatatcg aaacatggga
      481 agctggcaat gtggaggaaa ctatattcca ttgaaacatt tgtgacgaag aataataagc
      541 acatttatgc cagtgaaagt ggtggcacag taccaatgag gatatagaaa tatatcggag
      601 gaatagccac tagtcaaaag aaatcgttaa ttaaatgatg tatttcatag aatcgagtaa
      661 gacaaaataa acactacaac ctaagtaaac aaaggtagtg gtgctatgaa taaagtaaaa
      721 acaaataaaa cacttcgtca tagtgaatac tatggcatga cacaaatatt tgatgaactg
      781 tatgtaaaaa gtgataaaaa agaagttttt acagagctca tgacaattat tatgagtgat
      841 gaaaatattc gactagcata cagaaatatc aagaataaca agggcagtta tacagcgagt
      901 accgataaga aaagtattcg agacattaaa acactgtcag aagatacctt tctaactgag
      961 gttaaagttc tttttcgctg ttacaaacca cgagcagtta gacgtgtaga aattcccaaa
     1021 ccaaatggaa aaacacgccc tctgggcatt ccatcaatct gggataggtt aatacaacag
     1081 tgtattttac aagtcttaga accgatatgc gaagctaaat ttcacaatta tagttatgga
     1141 tttcgaccaa accgttccac acatcatgcg attgctacct gcaatcgact ggcaaatagg
     1201 gggaagctac actttgtagt agatgttgat attaaaggat tctttgataa tgtcaatcac
     1261 tctaaacttt tgcgtcaaat gtggacgatt ggaatccgtg ataaatggct attaggtatt
     1321 attcgatcca tgctaaatgc tcctatagtc ctaccaaccg gtaaagtcga acaccctaaa
     1381 aaaggtgtac ctcaaggtgg tgtcctatcc cctttactat ctaatatcat attgaatgaa
     1441 ttagattggt gggttgcttc acaatggcaa aataccacga ttgagacaga aaagattatg
     1501 gataggcgag ataaaggcaa aggcattgat aagactaatc agtatcgggc attaagacga
     1561 acaaacttaa aagaaatata cattgttcgg tatgctgacg acttcaaaat cttttgtaga
     1621 aaacgaagtc atgcagataa aatatttcac gcaacgaaaa tgtggcttaa agaacggtta
     1681 tcacttgaaa ttagcgaaga aaaatccaaa attgtcaatc tcaaaaaagg taaatcagaa
     1741 tatctaggat ttgaattaaa gctaatcaag aaaggtaaaa aatacgtagt cgaaagtcat
     1801 atgagcaaaa aatcaatgac aagagttaaa atccaattaa agaaacaatt acgtaaagtt
     1861 gccagaccga agaatcattg cgaacaagca aaagaaattg gattatataa ttctatggtg
     1921 attggaatcc atcaatatta tggaattgca acctgtgtaa atctcgattg cagtaaaata
     1981 gcctatacac taaaaccgtt catcaatcac aaactgccga caaagaaaca tggaaagata
     2041 ttaaatacat ttatcaaatc gaagtatgga aaaagtaaag aaatgcgatg gttgaataat
     2101 gtgccaattg taccgctcaa ctatgttcag tttagacttg ctgtccccct atcagaggga
     2161 acttgcaagt acactgaaag tggacgaagc aagatacaca gtaaattaaa attagaccta
     2221 gcccttttac ttcacatgat gagaaatagt aattatgaaa gaagtattga atttgtggat
     2281 aacagaatat ctaaatattc tgctcaaaag ggaaaatgtg ctatcacggg taaatttctc
     2341 gaatacgaag aaatccattg ccaccatatc gtaccagtaa aacaacaggg tacagacaaa
     2401 tacagcaacc tcattattat tcacaaaaat gttcacgcat tagtccatgc tatcgaagaa
     2461 aaaatcatac acaaatacct gaatgtatta aatctaacca atgaacaaat agaaaaacta
     2521 aatgaattac gtgtcaaagc tggtaatagt gttttaacag tttaacttga aaaatatatc
     2581 atacttaaca ttcttttgat ggaacgccgt atgaagtgaa agtttcatgt acggtgtgaa
     2641 gcgggggaaa atctggagat gacatcaaag gattacctat cgctattcaa ctttcgcgtg
     2701 gctttgggtc gtatgagtga actcggatat tccatgatgt ttggggaggt cgaaaaaaac
     2761 ttctttatga agcacatcaa aggtcgaggt tatgtggata caggtggcag tgtgattagt
     2821 gagttttata cgccacttgt accaaaaggg tatgatttct tagattcaat tagtaaatta
     2881 gaaaagatga atgattgagt gatcttcaat actgacttta tacttcaagt aacgtataat
     2941 acttttatat agtaggaaga aaggggataa ttaatatgga aagtaatgat tcttatttag
     3001 agtatgcaaa agcaaataag gataagttta tttcttatat tatcaaaggg aaatcccccg
     3061 aagttggacg agacgcagtt tttatggcag ggagtcctgg ggctggaaaa tcagaggttg
     3121 ccattggatt agctgagaat tatgataacc atgtgattat tgatgcggat tactttcgca
     3181 cccagtttcc tgattataat ggaaaaaact ctagtctctt tcaaaaagca tcttcttggt
     3241 tggtagagca ggcatttaga tatgtcttag atcatggcta ttcttttatt ctggatgcaa
     3301 cttttgcaat attaagtgct gaaaaaaata tctcgcgggc gttgaaaaaa cattttcgag
     3361 tgacgatttt ctatgtttat caagacccta aaatcgcttg gaaatttaca caagaacgtg
     3421 aaattgcaga gggacgtcat gtgccgaaaa aggcatttat taacgctttt ttcaaatcaa
     3481 gagagaacat tgcaaaagta aagagtaaac atccagatgt attgcttcat atcataataa
     3541 aagattatac gaataatatt tccgaagttc attatgctac ggataatcta gaattatttt
     3601 taccactagg gtatagtagg aaagatttgg aggttcaatt acatgactga gaaagaacaa
     3661 gtaacgaaaa ttgtaaagaa atacaataag agcattgcgg atttatcgga aaatgctact
     3721 gcaaaagagt tcaaaacggt gattaaatat gtagccgatc aggccaatga aaaacaacgg
     3781 aaattagttg gattaaataa aaaatagtag gacaaattaa taatgtatga gccaagagca
     3841 aaatgttctt ggcttttttc tttgcaatga atgaggtaat gtatgagatt tttagattta
     3901 ttcgccggta ttggtggctt tcgtttaggt atggaacagg cgagccatca ttgtatcggt
     3961 ttttgtgaaa ttgataaatt tgctagacgt agttataaag caattcatga tacaagtaag
     4021 gaggtggaaa tgcatgacat cacaagtgta tcagatgagt ttattcaatc cctcggacca
     4081 gtggacatcc tttgcggcgg atttccgtgc caagcttttt caattgcggg aaagcggcaa
     4141 agattttccg atactcgagg tactttattc tttgaaatcg ctcggttcac cactcttctc
     4201 cgacctaagc ttttattcct tgagaacgtc cgaggattgc tcaatcacga aggaggggct
     4261 acgttcgaga cgatcctccg aacgctggat ggattggggt atgacgtgga gtggtaggtg
     4321 cttaactcaa aggcctacat tccccaaagc cgggagcgga tattccttat cggacattct
     4381 cgagacgcat gtaccgaaca agtatttcct atcattggac cgtcttcaac atctgatcaa
     4441 aacatcagaa atttggtaaa tatcaatcct tccaatcgag gaatgggtgg acaagtctat
     4501 ggttcagatg gcgtagcgcc aaccttaaca ggtgatgaag gaatcaaaat cgcactgcca
     4561 gcaagggatg gaatctctgt agcaggacta ttaccgggaa agtttgaaca aggtaatcga
     4621 gtttatgaag ttactggaac cgcccccacc ttgtcaacga aacaaggtgg tacaaaaatc
     4681 atgattcgaa aaaatgaaac agaatatcaa gaggtgaagc cagaagacag tgtgaatctg
     4741 gcttttccta attccacaag tcgccgaggt cgtctgggaa aacagagtgt tcatacctta
     4801 ctaacaggag accaacaagc gatcgtcacg gatcagtatc aaattcggaa gctaacacct
     4861 agagagtgtt ggcgacttca gggctttccc gattgggctt ttgatcgaga ttctcaagtc
     4921 aattcagata gtcagttgta taagcaagcc ggaaactctg taacagttcc ggtcattttt
     4981 gatattgcca gaagattaaa agagttggat gtacatggtt tatgatgagt tgattggaga
     5041 aatttattgg gtgattggaa agattcagtc agacccagaa ttagaagaac aattgcgacg
     5101 gttaaaattt gatattcgca agaatggtgt caaagtcgct ggcgatccat atttaatgaa
     5161 tgaggagacg gatgctcgaa ttgaaataaa tcaagtaatt gcagaatttg agcggattgc
     5221 ggaccaagca aaagaaccag atattcgtca atacttgtta gaaatgaaag cagagttgga
     5281 agtaaatggt attacagatg agtaacctaa accaacacaa ctgactgtta ggcgctgacc
     5341 gcggaggatt cgcgaagcag aaatcctccg tggtgctgtg cctgttcatg gacggcgtta
     5401 gccgaccatg atgtctcacc cccgtatcta acaggggggt acaaaaacac aaaaaataag
     5461 taagcaacca ttaagaatgc ttgttgtatc aaagatagta agtaatttgt ataggtgtta
     5521 actcatttga aaaagttaac accttgaatt tggaggtgag aacttggcac aacggaattt
     5581 agattatcgg ctattaaaag accgacgcaa cgaatacggc gtttcacaaa ataaattggc
     5641 tacggcttgt ggacttagtc ggccgtactt aaatcagata gaaaatggtg gcgtaaccgc
     5701 atctataaaa accatgagga aaatttttga ccaactggaa agctttaatc ccgatttgcc
     5761 tttgacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg cacgaaaaat
     5821 tattcaagaa attctccatt tgaaatttga gtatatgctc catgaagatt atgcctttta
     5881 ctcctatcaa gaacagtatg tcatgggtga tattgtggtg atgttgtctc atgaagaaga
     5941 taaaggtgtg cttttagaat taaaggggcg aggttgccga caatttgaaa cttttttact
     6001 cgctcaaaag cgtagctggt acgacttttt cgaggattgt ctaaaggccg gtggcgtgat
     6061 gaaacgcttg gacttggcaa tcaatgatcg agtggggcta ttggatatac ctgagctaac
     6121 gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt acctttaaaa gttatcgttc
     6181 cggggaactt ttaaaggctg atgaaaagga cggcatgggc aataccttat atatcggcag
     6241 tctcaagagt gaggtttatt tctgtttgta tgaaaaagac tatgaacaat atatcaagtt
     6301 agggattcca ttagaccaaa ctgagacgaa aaatcgtttt gagattcgac tgaaaaatga
     6361 ccgtgctttt catgcgattc aagatttgtt aaaaggtcgt agcattgaga gcaccacgtt
     6421 ttccattatc aatcgttact tgcgatttgc ggataaagta gaaggtaaaa ggcgaaccaa
     6481 ctggcccttg aatgaacaat gggggcgttt tattggacgg aatcggaaag agattcaatt
     6541 aacttccgag ccaaaaccgt ataccattga acgaaccttg aattggctag gccgacaagt
     6601 cgcccctacg tggaaaatgg ctaaggagtt ggatcgattg aatcggacaa cctatatcca
     6661 ggatatggta cagaatgcac gcttatcaga ccgacataaa aagattttgg agcaacaaag
     6721 tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tcatgggaat tgtaaaagat
     6781 atttttttgg tatttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgaatgca
     6841 agtgcagcag cggatcgagc aatggaaaag aaaggaaagt gacaagatga atttcggaca
     6901 aaattt
//
LOCUS       DACEKG010000107         6711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEKG010000107 DACEKG010000000
VERSION     DACEKG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.113"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1014
                     /locus_tag="IW664_002507"
     CDS             <1..1014
                     /locus_tag="IW664_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0849829.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IW664_002508"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IW664_002508"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0849830.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3470..4063
                     /locus_tag="IW664_002509"
     CDS             3470..4063
                     /locus_tag="IW664_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ0849831.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="IW664_002510"
     CDS             4076..4975
                     /locus_tag="IW664_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0849832.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="IW664_002511"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="IW664_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0849833.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="IW664_002512"
     CDS             5817..6077
                     /locus_tag="IW664_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849834.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            6522..>6711
                     /locus_tag="IW664_002513"
     CDS             6522..>6711
                     /locus_tag="IW664_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0849835.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDE"
BASE COUNT         2460 a         1167 c         1382 g         1702 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
     6361 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
     6421 gtccttgtcg cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg
     6481 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     6541 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat
     6601 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
     6661 gcgggaaaaa atataccttg tcttatggcg attatatttt gattgatgag a
//
LOCUS       DACEKG010000108         6644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEKG010000108 DACEKG010000000
VERSION     DACEKG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.114"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..859)
                     /locus_tag="IW664_002514"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IW664_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IW664_002515"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IW664_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0849836.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IW664_002516"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IW664_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IW664_002517"
     CDS             complement(2143..2373)
                     /locus_tag="IW664_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0849837.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IW664_002518"
     CDS             complement(2603..3421)
                     /locus_tag="IW664_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0849838.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IW664_002519"
     CDS             3582..4271
                     /locus_tag="IW664_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0849839.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IW664_002520"
     CDS             4285..5787
                     /locus_tag="IW664_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0849840.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IW664_002521"
     CDS             5800..6276
                     /locus_tag="IW664_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0849841.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IW664_002522"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IW664_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DACEKG010000109         6452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEKG010000109 DACEKG010000000
VERSION     DACEKG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.115"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..550)
                     /locus_tag="IW664_002523"
     CDS             complement(95..550)
                     /locus_tag="IW664_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849842.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(704..1912)
                     /locus_tag="IW664_002524"
     CDS             complement(704..1912)
                     /locus_tag="IW664_002524"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0849843.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2024..2356)
                     /locus_tag="IW664_002525"
     CDS             complement(2024..2356)
                     /locus_tag="IW664_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0849844.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2504..3379
                     /locus_tag="IW664_002526"
     CDS             2504..3379
                     /locus_tag="IW664_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0849845.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3461..4042)
                     /locus_tag="IW664_002527"
     CDS             complement(3461..4042)
                     /locus_tag="IW664_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0849846.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4048..5307)
                     /locus_tag="IW664_002528"
     CDS             complement(4048..5307)
                     /locus_tag="IW664_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0849847.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5309..6355)
                     /locus_tag="IW664_002529"
     CDS             complement(5309..6355)
                     /locus_tag="IW664_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849848.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1798 a         1334 c         1100 g         2220 t
ORIGIN      
        1 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
       61 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
      121 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
      181 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
      241 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
      301 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
      361 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
      421 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
      481 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
      541 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
      601 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
      661 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
      721 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      781 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      841 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      901 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      961 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
     1021 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
     1081 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
     1141 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
     1201 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
     1261 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
     1321 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
     1381 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
     1441 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
     1501 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
     1561 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
     1621 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1681 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1741 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1801 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1861 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1921 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1981 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     2041 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     2101 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     2161 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     2221 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     2281 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     2341 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     2401 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     2461 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     2521 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     2581 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     2641 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2701 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2761 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2821 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2881 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2941 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     3001 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     3061 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     3121 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     3181 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     3241 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     3301 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     3361 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     3421 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     3481 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     3541 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     3601 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3661 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3721 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3781 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3841 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3901 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3961 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     4021 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     4081 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     4141 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     4201 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     4261 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     4321 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     4381 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     4441 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     4501 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     4561 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     4621 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4681 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4741 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4801 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4861 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4921 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4981 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     5041 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     5101 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     5161 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     5221 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     5281 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     5341 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     5401 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     5461 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     5521 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     5581 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     5641 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5701 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5761 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5821 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5881 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5941 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     6001 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     6061 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     6121 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     6181 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     6241 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     6301 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     6361 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     6421 aaagctcacc tcataacttg gaaataaata aa
//
LOCUS       DACEKG010000110         6369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEKG010000110 DACEKG010000000
VERSION     DACEKG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.116"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            594..1283
                     /locus_tag="IW664_002530"
     CDS             594..1283
                     /locus_tag="IW664_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0849849.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1270..2478
                     /locus_tag="IW664_002531"
     CDS             1270..2478
                     /locus_tag="IW664_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0849850.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2772..4043
                     /gene="serS"
                     /locus_tag="IW664_002532"
     CDS             2772..4043
                     /gene="serS"
                     /locus_tag="IW664_002532"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0849851.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4592..6076)
                     /gene="guaB"
                     /locus_tag="IW664_002533"
     CDS             complement(4592..6076)
                     /gene="guaB"
                     /locus_tag="IW664_002533"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0849852.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1938 a         1195 c         1230 g         2006 t
ORIGIN      
        1 tcaggagtgt ttcccgattc gtttttggtt gccgtagcaa cttttatatc atatcagaga
       61 gacaagtaat tgtcaactct tttttaaaag ttttttcgat ttcgtttttc gaaatcttcc
      121 agcaactcat ttatcataac ttatcgtttg ttatttgtca agaacttttt tgaagttttt
      181 ttcgatagaa tcaagcttcg caagtggttc ttttttaacg actcagttat cttatcatgt
      241 catttccaac atgtcaacaa cttatttttt attatttaat aagttattgg tgttcttttg
      301 aacaaataat aatatatcaa catcacatgt gaaagtcaat aagaaaatgc ttatttatca
      361 acatttctag cttacatgat cattggttag ttctggtcct ttttcgttcg tctttataac
      421 tccgagagac gtaaaatcct tttgttttca gtttaatgtt catgttcttg tcttattcac
      481 accaactaga tgtctcacct tctaaaattt tccttaacaa agtgctgctt ttatctattt
      541 ctgctttttt ttgctagaat aatagtaaca atatgactct ggaggattta tttatgaaaa
      601 ttttgattgc tgacgatgat aaagaaatcg ttgaattatt aagtatctat gttcacaatg
      661 aggggtatga agcagtaaag gcttatgatg ggaaagaagc attatctaaa atcagaacaa
      721 tccctgatat tgaattactt attttagata tcatgatgcc tgagatggat gggatgcaag
      781 tagtgaaaga gctacgaaaa gaatcccaaa taccaatcat tatgctgaca gcaaaaacta
      841 ctgatatgga taagatcaaa ggactggtcg ctggtgcaga tgattatgtg accaagccat
      901 ttaatccgtt agaagtaatg gctagagtaa agtctttatt acggcgtaca aaaatgcaga
      961 ttgccccaga tcagcctgat gtattagaag taaatgcatt aatcatcaat aaagactctc
     1021 atgaagttaa aacaatcgat gggaaagaaa tccagttaac agctttggaa ttcggtatcc
     1081 tctacttgct tgctagtcat cctaatcgag tatttagcgc ggatgaaatt tttgagcgtg
     1141 tttggaaaca agaaagtatt gtctctgcta aaactgtcat ggttcacgtt agtcatttaa
     1201 gagataagat tgaagaagca actggtggtg aaaaagtcat tcaaaccgtt tggggagtgg
     1261 gctataaaat tgacgcaaga tagtaaacga gcaactgaaa aacgccgtcg gatcaatttg
     1321 acttcaaaag aaatcagcga attgttagcg gaaggaatca tcacgattat ccttttgctt
     1381 ctattaaatg tttctattct ggttgttatc agttcggtga tcaacagttc accttctttg
     1441 acgaatgcga tttgggattc aaagaatatt tttgcagaac gattgaacac tgatttgttc
     1501 tggaacggcc ggaattttat tattcccttt ttctttctat tagatattgg tgttttgtat
     1561 tggcgcttga ttcgaagata tcgccaaatg caattgcggc atatcatcag cgaacttcac
     1621 tacatcgcaa acggaaatta tgatcatcgt atcccttttg aactgagtgg tgacttaagc
     1681 cgagtggtaa caagtatcaa tggattggtg gatagtaccg tagcagcgat tgaagacgaa
     1741 cgtaaaatcg aaaaatcaaa agatgaattg atcacgaatg tcagtcatga tatccgtacc
     1801 ccactcacgt ccatcattgg atacctagga ctgatagaag atggccagta tcactcagaa
     1861 gaagatcttt taaagtatac gcatactgct tatatcaaag caaagcagat gaaatcttta
     1921 gtggacgact tatttgaata cactaaggta cgccagcctg ctgtcccggt gaatttttct
     1981 gcctttgaca tgatccagtt gatcgaacag ttagcagctg attttgagtt ggaagcttct
     2041 aaaaaaaata tccaaatact tgttcaatca aaagtagact cccttatcat ggatggcgat
     2101 accgaaaaac ttgtacgtgt cttcaataat cttttgacga atgcgttgaa atacggaaaa
     2161 ggagcaacga agatcgtaat cgaagtagaa cgtatcggtt cagaagttgt tgcaacagtc
     2221 aaaaataacg gagcaatgat cccacaacag gcaatcgata atctattcga ccgtttttat
     2281 cgtgtagaag aatctcgttc acaagcaact ggaggaactg gacttggttt agcaatcgct
     2341 caaagcattg tggctttgca cggcggatac atttatgcaa aatcagataa gcaatggact
     2401 tctttcatca tgcaccttcc aatcaagaaa aatgaaaaat tacctattga tacacaggaa
     2461 gttattgacg aaagctgaaa tattcgctat tcttattaca attattagaa taaatgaaaa
     2521 acacagaacc cagtacactt tattcttttt cagagagctg atggcaggtg taaatcagca
     2581 aaggataatt tggaagttcc agttcgttta cgtgcccttt cgaaataaga aaggtcggtt
     2641 caacagccgt tatgtgttca acaagtgcag ctgacgctga acttgggtgg taccgcgaat
     2701 ttcagactga ttcgtcccaa gaataatggg atggatcagt ctttttttat tttcaaaaag
     2761 gaggaaatca tatgttagat gtaaaaatga tgcgacaaaa tattgaagaa gtacaagaaa
     2821 aattgatgac tcggggcgta aaaaaagaaa cattagctga attcatggaa ctagatgaaa
     2881 atcgtcgtaa gctactagtc aaaagtgaag aattaaaaaa atatcgaaat gatgtttctg
     2941 ctgagatcgc ccaattgaag cgtgcaaaag aagatgcttc tgctaaaatc gctgaaatga
     3001 aagaagtcgg tggcaacatc aaagcattgg atactgaaat tgctgaagtg gatgaaaaat
     3061 tgaaacaaat tgcaactact ttgccaaacc ttccccatga ttctgtgcca gttggaacag
     3121 atgaagagga caatgtagaa atccgtcgtt ggagcgaacc acgtacattt gcttttgaac
     3181 caaaaccaca ctgggaaatt gcagaaaatc ttgatatttt agattttgaa cgtggcgcaa
     3241 aagtagccgg tagccgtttt gtctattaca aaggattagg tgctcgatta gaacgtgctg
     3301 tctacaactt catgcttgat cagcatattt acgaacacgg ctatactgaa atgatcactc
     3361 cttatatcgt caacagtaaa gcgatgtttg gtacgggaca atttccaaaa ttcaaagaag
     3421 atgttttcca attggagaat actgatctaa cattaatccc aacagctgaa gtaccattaa
     3481 ctaactatta taatgatgaa attttagatg gtgcagattt acctatttat ttcactgcac
     3541 taagccctgc ttttcgttct gaagccggaa gtgctggtcg ggatacacga ggactgattc
     3601 gtcttcacca attcaataaa gtagaaatgg tcaaattttc ggatgtagag cattcttacg
     3661 aagaattgga aaaaatgaca gctgatgcag aagatatctt gaaaaaattg catttgcctt
     3721 atcgtgtgat ggccttatca actggagaca tgggcttttc tgcagctaaa acttatgact
     3781 tagaagtatg gatacctgca caagatactt atcgtgaaat cagttcttgt tctaactgtg
     3841 aagatttcca agcacgccgc gcaatgatcc gttatcgcga taatgatgga aaaatccact
     3901 atactcatac tctgaatggt tcaggacttg cagttggccg tacagtagct gccattttgg
     3961 aaaactacca aaatgaagat ggttctgtca cagttccaga agcgctcgtt ccttatatgg
     4021 ggaatctgaa agttattaaa taaagaggct gtgaaaaagc cgcttagttt cgagtattaa
     4081 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta ataccgagaa
     4141 actggctttt gaacaccatt tatcgagagg ttgtgaaatc agcgaattaa cctgagtatt
     4201 agagaaaaaa atggaaaaat agtccctcat attttaccgt ttgttgaact aataccgagg
     4261 gtcagctgat tgaacaccgt ttagtggaga ggtcgtaagc ctacccccca gctcctagtt
     4321 acaaggacaa attcaaaaaa cagctcttca tattttatga atgttgtact tgtgattcgg
     4381 aggtggcaag ggaacactgt ttattcaaaa agaggctggg acagaagcgt ttaactccaa
     4441 gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ttcttatttc
     4501 cgaaggagtt gcttctactt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
     4561 ctttactttt gtcccacgct ctttaatgtt tttaagattc aactgaatag tttggtgctt
     4621 cttttgtgat ttgtacatca tgagggtgtg attcttttaa tccactgccg ctcatttgga
     4681 taaattgcgc ttcatcacgt aattgctgta gattggctgc cccaacataa cccattcctg
     4741 aacgcaaacc gccgatcatt tggaaaataa tatctgcaac acttccttta taagcaaccc
     4801 gaccttcgat tccttctggt actaatttat ttgcttcatt gacactacct tggaagtaac
     4861 ggtcttttga acctttttcc atcgcgccta atgagcccat acctcggtat gttttgaatc
     4921 gacgtccttg gaagatttcg aattcacctg gtgattcatc tgtccctgcc aacatacttc
     4981 ctagcatcac tgcatgtccg ccggcagcta atgctttgac gatgtcacca gaatatttga
     5041 tccctccgtc tgcaataatc gctttgccat attgacgagc aactgatgct gcatcataaa
     5101 tagctgtgag ctgaggtaca cctacaccgg ctaccacacg tgtcgtacaa atcgatcctg
     5161 gtccgatacc aacttttacg acatctacac ctgcatcata taaagctttt gtcgcttcag
     5221 ccgtcgcaac gtttcctgca atcaatgttg catctgcaaa agttgaacgg atttcttgaa
     5281 tttttcggat cacacctgca ctatgtccat gagctgtatc gatgatgatt gcatctgccc
     5341 ctgcttctaa taaagcattc gcacgctcaa atgtatcgct tgttacacca acggctgctg
     5401 ctacaagcaa tcgaccatgt tcatcttttg ctgcatttgg aaattcgatc actttctcga
     5461 tgtctttgat cgtgatcaat ccgcttaaac gaccttcgtt atctacgatt ggtaattttt
     5521 cgattttatg tttttgcaaa atcttttctg catcttttag agaggtacct actggggcag
     5581 tcactaagtt gtcttttgtc atcacatcag caatcggcat gtgataatct gttacaaaac
     5641 gcatgtcgcg gtttgtaatt atccctacca attttctgtt ttcaagtgtt tcaacgattg
     5701 gcacaccact gatacgatat ttgctcatca attcttctgc gtctgctaca agatgctgtg
     5761 gcgttaagaa gaacggatca atgattacgc cactttcaga acgtttgact tttcttactt
     5821 catccgcttg ctggctgatc gtcatgtttt tatggatcac acctagtccg ccttgtcttg
     5881 ccatagcaat ggccatcttg ctgtcggtaa cagtatccat acttgcactg atgattggaa
     5941 tattcaactt aatattttta gctaattgta cgctcatatc cacttcgttt ggtaacacgt
     6001 gactttctgc tggtatcaat aagacatcat caaaagtaaa tccttttttc gtgaactttg
     6061 tttcccagtt ggacattttt agtaccactc ctctaattta gtttatctat taaaataatt
     6121 gattttttca tttaagtcaa tgatctactt ggaatcaagt tagctttctc atagaatacc
     6181 aatataaagc gtttacatct gtgtgtatta aaagagattg taaaaaagct gtcctgcatc
     6241 aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgaattgg ttgggtttat
     6301 taccgcagat gcaaactttt gaacaccgtt tagtggagag gctgtgaaaa agctgcttag
     6361 tttcgagta
//
LOCUS       DACEKG010000111         6172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEKG010000111 DACEKG010000000
VERSION     DACEKG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.117"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW664_002534"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW664_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0849853.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IW664_002535"
     CDS             complement(2203..4389)
                     /locus_tag="IW664_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0849854.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW664_002536"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW664_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0849855.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IW664_002537"
     CDS             complement(4611..5051)
                     /locus_tag="IW664_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0849856.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IW664_002538"
     CDS             complement(5125..5664)
                     /locus_tag="IW664_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ0849857.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1597 a         1388 c         1088 g         2099 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccaacccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgccc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aa
//
LOCUS       DACEKG010000112         6100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEKG010000112 DACEKG010000000
VERSION     DACEKG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.118"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..1106
                     /locus_tag="IW664_002539"
     CDS             132..1106
                     /locus_tag="IW664_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0849858.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="IW664_002540"
     CDS             1088..1549
                     /locus_tag="IW664_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0849859.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="IW664_002541"
     CDS             1599..2063
                     /locus_tag="IW664_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0849860.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="IW664_002542"
     CDS             2077..2826
                     /locus_tag="IW664_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0849861.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="IW664_002543"
     CDS             2826..3656
                     /locus_tag="IW664_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0849862.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="IW664_002544"
     CDS             3656..4303
                     /locus_tag="IW664_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0849863.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="IW664_002545"
     CDS             complement(4401..5177)
                     /locus_tag="IW664_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0849864.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="IW664_002546"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="IW664_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(5519..6100)
                     /locus_tag="IW664_002547"
                     /pseudo
     CDS             complement(5519..6100)
                     /locus_tag="IW664_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         2057 a          851 c         1089 g         2103 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 tttattggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa ataatgggtt
     5521 actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta tggcttctat
     5581 ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct agtgattctt
     5641 cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttcttg aaagattcga
     5701 ttaaattagt tgagtaaatg cttcggcgga taccaggcgg aaactcataa aaagtcaata
     5761 aatcttgatt ttttatcagt gactgcgtca ctttaggata agttttttgc tacttctcaa
     5821 tcatactccc taaaaaggta tttgcctctt cctttgagct agcttgataa acagccttaa
     5881 aatcatcata gatttctttt cgatctttga cacgtacttt atgagcaata ttacgggaaa
     5941 cgtgaacaca gcaatgttgg tatttagctt taggataaat ctggtgaatc gtatctttca
     6001 tgccttttaa gccatcggta ataaacagta agacatcatg aacccctctg gagtaaatgt
     6061 cctgtaacaa ctcattccaa acgtaggtcg attcagtcgg
//
LOCUS       DACEKG010000113         6068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEKG010000113 DACEKG010000000
VERSION     DACEKG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.120"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            832..1566
                     /locus_tag="IW664_002548"
     CDS             832..1566
                     /locus_tag="IW664_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0849865.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IW664_002549"
     CDS             1582..2751
                     /locus_tag="IW664_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0849866.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IW664_002550"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IW664_002550"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0849867.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IW664_002551"
     CDS             3841..4767
                     /locus_tag="IW664_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0849868.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IW664_002552"
     CDS             4786..6036
                     /locus_tag="IW664_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0849869.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1247 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DACEKG010000114         5996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEKG010000114 DACEKG010000000
VERSION     DACEKG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.121"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..59
                     /locus_tag="IW664_002553"
     CDS             <1..59
                     /locus_tag="IW664_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849870.1"
                     /translation="NPSDKSIYHWDFYALLGF"
     gene            75..1994
                     /gene="tet(M)"
                     /locus_tag="IW664_002554"
     CDS             75..1994
                     /gene="tet(M)"
                     /locus_tag="IW664_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0849871.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2092..3465
                     /gene="tet(L)"
                     /locus_tag="IW664_002555"
     CDS             2092..3465
                     /gene="tet(L)"
                     /locus_tag="IW664_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0849872.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            4029..5291
                     /locus_tag="IW664_002556"
     CDS             4029..5291
                     /locus_tag="IW664_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0849873.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5536..>5996
                     /locus_tag="IW664_002557"
     CDS             5536..>5996
                     /locus_tag="IW664_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0849874.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK"
BASE COUNT         2049 a          862 c         1244 g         1841 t
ORIGIN      
        1 aaaatcccag tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa
       61 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcgggaa
      121 aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg
      181 tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa
      241 ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc
      301 caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa
      361 ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac
      421 ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg
      481 atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac
      541 agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg
      601 atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag
      661 aagcattgga actcgaacaa gaggaaagca taagatttca gaattgctcc ttgtaccctg
      721 tttatcatgg aagcgcaaaa agcaacatag ggattgagca gcttatagaa gtgataacga
      781 ataaatttta ttcatcaaca tacagaaaga agtctgaact ttgcggaaat gtcttcaaaa
      841 ttgaatattc ggaagaaaga caacgtcttg catatgtacg cctttatggc ggaatcctgc
      901 atttgcggga ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata
      961 cttcaataaa tggtgaatta tgtaaaattg ataaggctta ttccggggaa attgttattt
     1021 tgcaaaatga gtttttgaag ctaaatagtg ttcttggaga tacaaagcta ttgccacaga
     1081 gagagagaat tgaaaatccg ctccctctgc tgcaaacaac tgttgaaccg agcaaacctc
     1141 aacaaaggga aatgttactt gatgcacttt tagaaatctc cgacagtgac ccgcttctac
     1201 aatattatgt ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa
     1261 tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc
     1321 ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag
     1381 tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa
     1441 gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg
     1501 cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg
     1561 attgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt
     1621 ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt
     1681 tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca
     1741 acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca
     1801 ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct
     1861 ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg
     1921 aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca
     1981 ataaaataac ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat
     2041 gaaatatttt ttaataaaga tttgaattaa agtgtaaagg aggaattaat tatgaagtgt
     2101 aatgaatgta acagggttaa atacaaccaa attttaattt ggctttgcgt tttatctttt
     2161 tttagcgtat taaatgaaat ggttttgaac gtctcattac ctgatattgc aaatgatttt
     2221 aataaaccac ctgcgagtac aaactgggtg aacacagcct ttatgttaac cttttccatt
     2281 ggaacagctg tatatggaaa gctatctgat caattaggca tcaaaaggtt actcctattt
     2341 ggaattataa taaattgttt cgggtcggta attgggtttg ttggccattc tttcttttcc
     2401 ttacttatta tggctcgttt tattcaaggg gctggtgcag ctgcatttcc agcactcgta
     2461 atggttgtag ttgcgcgcta tattccaaag gaaaataggg gtaaagcatt tggtcttatt
     2521 ggatcgatag tagccatggg agaaggagtc ggtccagcga ttggtggaat gatagcccat
     2581 tatattcatt ggtcctatct tctactcatt cctatgataa caattatcac tgttccgttt
     2641 cttatgaaat tattaaagaa agaagtaagg ataaaaggtc attttgatat caaaggaatt
     2701 atactaatgt ctgtaggcat tgtatttttt atgttgttta caacatcata tagcatttct
     2761 tttcttatcg ttagcgtgct gtcattcctg atatttgtaa aacatatcag gaaagtaaca
     2821 gatccttttg ttgatcccgg attagggaaa aatatacctt ttatgattgg agttctttgt
     2881 gggggaatta tatttggaac agtagcaggg tttgtctcta tggttcctta tatgatgaaa
     2941 gatgttcacc agctaagtac tgccgaaatc ggaagtgtaa ttattttccc tggaacaatg
     3001 agtgtcatta ttttcggcta cattggtggg atacttgttg atagaagagg tcctttatac
     3061 gtgttaaaca tcggagttac atttctttct gttagctttt taactgcttc ctttctttta
     3121 gaaacaacat catggttcat gacaattata atcgtatttg ttttaggtgg gctttcgttc
     3181 accaaaacag ttatatcaac aattgtttca agtagcttga aacagcagga agctggtgct
     3241 ggaatgagtt tgcttaactt taccagcttt ttatcagagg gaacaggtat tgcaattgta
     3301 ggtggtttat tatccatacc cttacttgat caaaggttgt tacctatgga agttgatcag
     3361 tcaacttatc tgtatagtaa tttgttatta cttttttcag gaatcattgt cattagttgg
     3421 ctggttacct tgaatgtata taaacattct caaagggatt tctaaatcgt taagggatca
     3481 actttgggag agagttcaaa attgatcctt tttttataac aggaattcaa atctttttgt
     3541 tccattaaag ggcgcgattg ctgaataaaa gatacgagag acctctcttg tatctttttt
     3601 attttgagtg gttttgtccg ttacactaga aaaccgaaag acaataaaaa ttttattctt
     3661 gctgagtctg gctttcggta agctagacaa aacggacaaa ataaaaattg gcaagggttt
     3721 aaaggtggag attttttgag tgatcttctc aaaaaatact acctgtccct tgctgatttt
     3781 taaacgagca cgagagcaaa accccccttt gctgaggtgg cagagggcag gtttttttgt
     3841 ttcttttttc tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg gtcggcactg
     3901 ccgacagcct cgcagagcac acactttatg aatataaagt atagtgtgtt atactttact
     3961 tggaagtggt tgccggaaag agcgaaaatg cctcacattt gtgccaccta aaaaggagcg
     4021 atttacatat gagttatgca gtttgtagaa tgcaaaaagt gaaatcagct ggactaaaag
     4081 gcatgcaatt tcataatcaa agagagcgaa aaagtagaac gaatgatgat attgaccatg
     4141 agcgaacacg tgaaaattat gatttgaaaa atgataaaaa tattgattac aacgaacgtg
     4201 tcaaagaaat tattgaatca caaaaaacag gtacaagaaa aacgaggaaa gatgctgttc
     4261 ttgtaaatga gttgctagta acatctgacc gagatttttt tgagcaactg gatccaggag
     4321 aacaaaaacg attttttgag gaaagttata aattattttc cgaacgatat ggcaagcaaa
     4381 atattgctta tgcaacagtt cataatgatg agcaaacccc tcacatgcat ttaggtgttg
     4441 tgcctatgcg tgatggaaaa ctgcaaggaa aaaatgtgtt taatcgtcaa gaactgttat
     4501 ggctacaaga taaattcccc gagcatatga aaaaacaggg ttttgagttg aagcgtggtg
     4561 aacgtggctc tgaccgtaaa catattgaga cagctaaatt taaaaaacaa actttggaaa
     4621 aagagattga ttttctagaa aaaaatttag cagttaaaaa agatgaatgg actgcttata
     4681 gcgataaagt taaatcagat ttagaagtac cagcgaaacg acacatgaaa agtgttgaag
     4741 tgccaacggg tgaaaagtcc atgtttggtt tgggaaaaga aataatgaaa acagaaaaga
     4801 aaccaaccaa aaatgttgtt atatcggagc gtgattataa aaacttagtg actgctgcga
     4861 gagataacga taggttaaaa cagcatgtta gaaatctcat gagtactgat atggcgagag
     4921 aatataaaaa attaagtaaa gaacatgggc aagttaaaga aaaatatagt ggtcttgtag
     4981 agcgatttaa tgaaaatgta aatgattata atgagttgct tgaagaaaac aagtctttaa
     5041 agtctaaaat aagcgattta aagcgtgatg tgagtttaat ctatgaaagc actaaggaat
     5101 tccttaagga acgtacagac ggcttaaaag cctttaaaaa cgtttttaag gggtttgtag
     5161 acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca cgatttagaa cctaaaaaga
     5221 acgaatttga actaactcat aaccgagagg taaaaaaaga acgaagtcga gatcagggaa
     5281 tgagtttata aaataaaaaa agcacctgaa aaggtgtctt tttttgatgg ttttgaactt
     5341 gttctttctt atcttgatat atatagaaat aacgtcattt ttattttagt tgctgaaagg
     5401 tgcgttgaag tgttggtatg tatgtgtttt aaagtattga aaacccttaa aattggttgc
     5461 acagaaaaac cccatctgtt aaagttataa gtgaccaaac aaataactaa atagatgggg
     5521 gtttctttta atattatgtg tcctaatagt agcatttatt cagatgaaaa atcaagggtt
     5581 ttagtggaca agacaaaaag tggaaaagtg agaccatgga gagaaaagaa aatcgctaat
     5641 gttgattact ttgaacttct gcatattctt gaatttaaaa aggctgaaag agtaaaagat
     5701 tgtgctgaaa tattagagta taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga
     5761 gtgtggtttt gtaaatccag gctttgtcca atgtgcaact ggaggagagc aatgaaacat
     5821 ggcattcagt cacaaaaggt tgttgctgaa gttattaaac aaaagccaac agttcgttgg
     5881 ttgtttctca cattaacagt taaaaatgtt tatgatggcg aagaattaaa taagagtttg
     5941 tcagatatgg ctcaaggatt tcgccgaatg atgcaatata aaaaaattaa taaaaa
//
LOCUS       DACEKG010000115         5934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEKG010000115 DACEKG010000000
VERSION     DACEKG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.122"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(76..1170)
                     /locus_tag="IW664_002558"
     CDS             complement(76..1170)
                     /locus_tag="IW664_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0849875.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IW664_002559"
     CDS             1359..2120
                     /locus_tag="IW664_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0849876.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW664_002560"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW664_002560"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0849877.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IW664_002561"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IW664_002561"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0849878.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IW664_002562"
     CDS             complement(3760..4773)
                     /locus_tag="IW664_002562"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0849879.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IW664_002563"
     CDS             4938..5858
                     /locus_tag="IW664_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0849880.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1807 a         1186 c         1064 g         1877 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtcccacg ctct
//
LOCUS       DACEKG010000116         5907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEKG010000116 DACEKG010000000
VERSION     DACEKG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.123"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..667)
                     /locus_tag="IW664_002564"
     CDS             complement(95..667)
                     /locus_tag="IW664_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0849881.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW664_002565"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW664_002565"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0849882.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="IW664_002566"
     CDS             complement(1352..1867)
                     /locus_tag="IW664_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ0849883.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="IW664_002567"
     CDS             complement(1869..2153)
                     /locus_tag="IW664_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0849884.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="IW664_002568"
     CDS             complement(2187..3515)
                     /locus_tag="IW664_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0849885.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="IW664_002569"
     CDS             complement(3529..3984)
                     /locus_tag="IW664_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0849886.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..>5907)
                     /locus_tag="IW664_002570"
     CDS             complement(3987..>5907)
                     /locus_tag="IW664_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0849887.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2005 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatttt
//
LOCUS       DACEKG010000117         5665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEKG010000117 DACEKG010000000
VERSION     DACEKG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.124"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..194)
                     /locus_tag="IW664_002571"
     CDS             complement(<1..194)
                     /locus_tag="IW664_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849888.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(737..2887)
                     /locus_tag="IW664_002572"
                     /pseudo
     CDS             complement(737..2887)
                     /locus_tag="IW664_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3033..3608
                     /locus_tag="IW664_002573"
     CDS             3033..3608
                     /locus_tag="IW664_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0849889.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3822..4517
                     /gene="vanR"
                     /locus_tag="IW664_002574"
     CDS             3822..4517
                     /gene="vanR"
                     /locus_tag="IW664_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0849890.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4495..5649
                     /locus_tag="IW664_002575"
     CDS             4495..5649
                     /locus_tag="IW664_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0849891.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1770 a         1092 c         1073 g         1730 t
ORIGIN      
        1 tgttcaaaat ctaaaatcaa cacatcatgg ggagtgtgat aataaatatt ttcccttaat
       61 gtaccaacaa tggtaattcg gtcaattgac cattgcattt ttaagggggt gcacatagtt
      121 tgtgcattta accgcctgtt agaaatacgg ttaagttttt cccactcatt tacgattatg
      181 ccttttttac tcaaaaatag ctcctcctca acgaaaaaag agattcttgg aaagaatttc
      241 cagagttcac gtaactctaa aaatccacat ccaaaaatct ctagcttgaa gaaacctaaa
      301 aatattcgta taatgaactt gagttacgga actaggtgaa gtagtttccg taaaggtcga
      361 gagtgcgtca acactgctcg actatttttt tactcttttt tctcattttc tttgattata
      421 gcactcctct agaaaaaata aaaagacctc agggaatttc attccccgaa ccccttgagc
      481 ctcctcctgc cgaaatagaa tggcgctgcc agcatttatt tcgttcagct atcggctaca
      541 caatctaaca cgaccgttcc gctaccagtc gccttacagg ctccattttc gctcctctaa
      601 cgtgtttcac cgattgtgcc gataactaaa cgaaataaac gctaaaaccg ctcagaacgc
      661 aaaatatgcc attttagagc tattgtagaa aatatagttt gaaatgcaat tttcttcttt
      721 cagcttttta tttcccactt gtaaatcggt agatggtggc tccttttcca gttccataat
      781 gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata ccatctgacg
      841 aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga aagtttacta
      901 atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat acattggcta
      961 gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca atattcattc
     1021 ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt tttcgattat
     1081 tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc atgagtaaat
     1141 cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt tttgcttctt
     1201 ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga gaaaccccat
     1261 ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg ctggttctct
     1321 ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc ttcgattgat
     1381 tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg ctgccaacaa
     1441 tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc atttcatagt
     1501 aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat cgttttgaaa
     1561 taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc attcctcgga
     1621 taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat tccaatactc
     1681 ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt aagtctaaat
     1741 aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag acaaaggtat
     1801 tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct cttgctttaa
     1861 ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt ttaccgtttt
     1921 gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt atttgcctat
     1981 catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa tagatggtta
     2041 atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg tatcttgagc
     2101 ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc actgtttcta
     2161 aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag
     2221 gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg gatggatgct
     2281 gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat ttactaaccg
     2341 tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt tcaagtgtag
     2401 tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca tgcagtagat
     2461 gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc attcgatatt
     2521 cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa
     2581 gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca atctgtttcg
     2641 atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc caaccgggat
     2701 accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc cttcgcttat
     2761 taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc cattcatctt
     2821 cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta cctctcgcaa
     2881 ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat tatatatcaa
     2941 tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt aagagcgtct
     3001 cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat attcgtgtca
     3061 gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc ggaatggata
     3121 ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt
     3181 tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta
     3241 gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat
     3301 cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta
     3361 ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag
     3421 ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa
     3481 atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc
     3541 aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga
     3601 ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta
     3661 taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca
     3721 tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg
     3781 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     3841 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     3901 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     3961 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     4021 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     4081 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     4141 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     4201 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     4261 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     4321 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     4381 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     4441 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     4501 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     4561 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     4621 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     4681 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     4741 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     4801 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     4861 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     4921 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     4981 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     5041 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     5101 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     5161 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     5221 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     5281 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     5341 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     5401 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     5461 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     5521 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     5581 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     5641 aggtcctaag agatgtatat aattt
//
LOCUS       DACEKG010000118         5608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEKG010000118 DACEKG010000000
VERSION     DACEKG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.125"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            30..446
                     /locus_tag="IW664_002576"
     CDS             30..446
                     /locus_tag="IW664_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849892.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            428..700
                     /locus_tag="IW664_002577"
     CDS             428..700
                     /locus_tag="IW664_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849893.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            867..1268
                     /gene="tnpA"
                     /locus_tag="IW664_002578"
     CDS             867..1268
                     /gene="tnpA"
                     /locus_tag="IW664_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0849894.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            1280..2425
                     /gene="tnpB"
                     /locus_tag="IW664_002579"
     CDS             1280..2425
                     /gene="tnpB"
                     /locus_tag="IW664_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0849895.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            2663..2926
                     /locus_tag="IW664_002580"
     CDS             2663..2926
                     /locus_tag="IW664_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0849896.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            2920..3270
                     /locus_tag="IW664_002581"
     CDS             2920..3270
                     /locus_tag="IW664_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0849897.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(3294..3908)
                     /locus_tag="IW664_002582"
     CDS             complement(3294..3908)
                     /locus_tag="IW664_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0849898.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            4358..>5608
                     /locus_tag="IW664_002583"
     CDS             4358..>5608
                     /locus_tag="IW664_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0849899.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT         2029 a          856 c         1052 g         1671 t
ORIGIN      
        1 aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa ttactatgac gaatttgtca
       61 aattaccttt ggataaaatg gcacaaaaaa tggaggatat gacattccta tacaatgaga
      121 cacgtgttcc aaaaaaacat tataaggaaa gattgtcggt agctgtggaa gaaatgattg
      181 aatcaggtgt cgaaatgaat ctcattgcga cgtattatcg aacactggaa gaactgaaaa
      241 agcaaaatgc aaaatggttt ttccaagcct tactatgctt ggaagtaggc gtcaaaccaa
      301 gcacaatcaa accttctgaa taccaagctt tagaactaac gtatgcgaaa tttatcgaaa
      361 cgaaaaaagc taagactgtt tcaagtgaat ggttagatta ttttgaaaat atcaataagt
      421 atggcgcatg ttacatcatg aagtaatcag acggattcaa aaagacttta gttgtatgga
      481 agaaatgaag tactttgttc aaaatgttga agaacagtta tatttggaaa gcgaaacgag
      541 tcaaacaaag gtcacagtca gccataatcg tttagatgtg tataaacgtc gtcctgaatc
      601 attaaaaaaa ggagcggagc ttctacgact agaaacagaa aaatacgtca tgaatgttta
      661 cctagaaaaa agtgagttgg tcgaaaattt tatggactag ccgagaacac ccgtgatttt
      721 aatcatggga tgaatcggat tcagttgtga cctagcttta gctaggttaa acaactgaca
      781 aatataaact aaataatagt gaaatgtctt gcaataattg attttgtaga aatacatata
      841 ttaaaataat tgcgaggtgt ttttgtatgt cagaaattca ttatggacgg ggttatgtct
      901 attctattca atatcatctt gtttggtgtg taaaatatcg acatgatgtt ttgtatggtc
      961 aggtagatat tgatgtcaaa cagtttttga aacaaattgc tgatgataat aatataaaaa
     1021 ttatagaaat agaatcagat aaagaccata ttcatttact cattgaatgc accccacaac
     1081 actatattcc tagtattgtt aaagtattta aaggcgcatc cgctagactg ctgtttaaaa
     1141 aacatcctga acttaaacaa ctcctttggg gtggacatct ttggaatcca agctactttg
     1201 tagctactgt tagcgaaaat acagaagaac aaattagaaa atatatacaa aatcagaaaa
     1261 agaaatgagg tgaatccgta tgctacaaca tagggcttat aaatttagaa tctatcctaa
     1321 tcaagaacaa gaaatattaa tgactaaaac tattggctgt tcaagatttg tctataatta
     1381 ttttttgaat ttgtggaatc atgaatatac gactactgga aaaggattat cttataattc
     1441 ttgttctgcc atgcttccac aaatgaaaag aaatgcagag acaatttggt taaaagaagt
     1501 tgacagtatt gcgattcaat cctcattgaa aaatctttct gatgcttttt ctcgcttctt
     1561 taaaaagcaa aataaacgac cacaatttaa aagtaaaaaa catcctatac aaagttatac
     1621 aactaaaaat gtgaataata gtatcgaaat tagaaacaat ttcgtaaaac tacctaagtt
     1681 aggatttgtt aaatttgcga aaagtcaaga gcccaaaggg cgaatcttaa atgctacgat
     1741 tcgaaaaaat tcgagtggta aattttttgt ttccatcctg tgtgaagaag aaatagttga
     1801 gtttcctaaa tctaattccg ctattggaat cgaccttggt ataactgatt ttgcaatcct
     1861 ttctgatggt caaaagattg ataacaataa attcacgtca aaaatggcaa aaaaactcaa
     1921 acgtgaacaa cgtaagttgt caaaacgtgc attactagct aaaaagaaag gcgtcaatct
     1981 ctttgaagct aaaaattatc aaaaacaaaa acgaaaagta gcacgactac atgaaaaagt
     2041 aatgaatcaa cgcaatgact ttttaaacaa gttgagcaca gagattatca aaaaccacga
     2101 tattatctgt atagaagact taaatacaaa aggtatgttg cgtaatcata aactagcaaa
     2161 aagcatttct gatgtgtctt ggtctagttt tgtgactaag ttacaataca aagctaactg
     2221 gtacggtcgg aaaatcatca aagtagataa atggtttcca tctagtcaaa tctgttcaaa
     2281 ttgtggacac aaagacggca agaaatctct agaaattcga gaatggactt gtcctgtttg
     2341 tcatgctcat catgaccgag atatcaatgc tagtatcaat attttgaccg aaggtctaag
     2401 attacatgtc atgggattag cttagatcca atcatcaaaa accgtaggaa ctacggggat
     2461 agcttggtaa ataagagaaa ccgctgtgag taaagaaata cgcttgcaag tatgctctat
     2521 tcccaagaat ctcgtgactt tagtcatgag aggttcaagg attcatggaa tgttaatcta
     2581 atttaataga aaaggataat taagtatata cgttttttct tatgtatgtt atactatgta
     2641 tatacagaaa gaggtgtcta ctatggaaat gttacctata aaaaaatggg gaaatagtaa
     2701 tggccttaga ttgccaaagt atattatgga atatcttgga attcatacag aagacaaagt
     2761 gaagataata caagaagaag taaacggtca gaagcggcta attatcgaag cggctaatat
     2821 agaaaatgaa ttaacaattg aacagttatt tgaaaattac actgatgaaa gaaatcatgt
     2881 gacgattcaa aatttaggtg aagcagtagg taatgaaaaa tggtgagaaa acctagacaa
     2941 ggcgatattc tattattgaa tacagcacct cgatctggac acgaacagac aggtaaaaga
     3001 ccttatattg ttttaagtca tgatattatt gcggattata gtaacgttgt tacagttgct
     3061 ccaatttcta gtacaactag aaactatccg ctatatgtaa atataaactc agaatatgaa
     3121 atgaagacaa caggaaaagt attattagat caactaacga caattgatta tgaagccaga
     3181 gagtgtattt ttttggaaaa agcgcatgat agtttagttg aggagttatt gatgaaagtt
     3241 agaacagttt ttcaaaaagt aagcaaataa aatagaaaca gcagcatgat caactattcg
     3301 tgattgacca tgctgtcttt ttttattcta tatacggttt gtctcgtaac attgtagtct
     3361 tttgcaatct tagcgatcgc aacacctttt tttagatcct caacgatgtt tttataaaca
     3421 agacgacgtt ggggatcttt tgcattggca ctgtatagct taggacgtcc tttataaaca
     3481 ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc gtttagattc ggtacgttct
     3541 tgttcagcga tcatggccaa aatttgaatg atcaaatctt taatgaattt atccaggaga
     3601 ggattgccaa ttgcttcggc catgatcggt agacttgtga tgatcagctg cacatttttc
     3661 ttcttcaagt aattgactga agcgatgatc tcgtcgtaat ttcttccaag tcgatcaata
     3721 gcttccacaa taaaaatatc ttgatctcgg acgaaatcca gtgcttcttg aaaaacaggt
     3781 cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa ttctctctgc accgaatttt
     3841 ttcatttctt caatctgccg ttgttcattt tgatcgatgg atgagacacg tatgtatgca
     3901 actctcaaaa tttttaccta ctttctgcaa ctatttaaaa aacaccctaa tcatacacga
     3961 gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag ggtatacctt atatagacaa
     4021 aaactaatga tggaggaaat agtatgagaa ttttttgtaa gtgtctactg ataacttatt
     4081 ctcaaatgtt gtaaggattt tattcatttg ttaatattaa agaacggaac tccaaaaaag
     4141 tccgtactct aatttgcagt ctctcactct tttagagtga gagtttttat tagattttca
     4201 aattaattag taaagtgata gaataaattg atcatacaaa tcaaaagaag aagcagggtg
     4261 atagcatacc tatgcttctt tttttatgta tttagctatt tcttctttac aatatcgaac
     4321 atatgtttgt ataatgtgaa agaatggagt gttatttatg atggattatt caaaagagcc
     4381 agtaaatgat tactttttaa tcgatatgaa aagcttttac gcaagtgtcg aatgtataga
     4441 aagaaattta gatccattaa caacagaact tgttgttatg agtcgagctg ataatactgg
     4501 ttcaggattg atattagctt cttctcctga agcaaaaaag cggtatggta ttacaaatgt
     4561 gagtagacca cgtgacttac cacaaccatt tcctaaaaca ctacacgttg ttccaccacg
     4621 tatgaaccta tatatcaagc gaaatatgca ggtaaataat attttcagaa gatatgtggc
     4681 tgatgaagat ctactgattt actcgatcga tgaatcaatc ttaaaagtga cccgatcact
     4741 gaatcttttt acgactgaag gaacacgaag tcaacggaga aagaagctgg ctcaaatgat
     4801 ccaggagaat attaaagaag agctaggatt gattgctaca gtaggtgtcg gagataatcc
     4861 ccttttggct aaactagcct tagacaacga agcaaagcat aatgaaggat ttgttgcgga
     4921 atggacgtac gaaaacgttc ctgaaaaagt atggaatatt cctgaaatga cggacttttg
     4981 gggaattggc tctcgaatga aaaaaagact caatcagatg gggattatga gcattagaga
     5041 tcttgctaac tggaatcctt atacgattaa gaatagacta ggtgtgattg gcttacagct
     5101 gtacttccat gctaacggaa tcgatcgaac agatatagca atcccgccag aaccgacaaa
     5161 ggaaaaatct tatggtaatt cacaagtgtt accacgagat tatactagaa gaaatgaaat
     5221 cgagctggtg gtaaaagaaa tggcggaaca agtagccatt cgtatcaggc agcataattg
     5281 taaaacagga tgtgttcatt taaacattgg tacgtcgatc ttagaaacga gaccaggatt
     5341 ttcacaccag atgaaaatac ctatcacaga taatacaaag gaattgcaga attattgtct
     5401 tttcttgttt gataagtatt atgaaggaca agaagtaagg cacgttggaa taacctatag
     5461 caagcttgtg tacaccgatt cgcttcagct ggatttattt agtgatccac aaaagcaaat
     5521 taacgaagaa aatttagata aaattattga caagattcgg caaaaatatg gttttacgtc
     5581 gattgtacat gcttcttcaa tgttagaa
//
LOCUS       DACEKG010000119         5356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEKG010000119 DACEKG010000000
VERSION     DACEKG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.126"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..936
                     /locus_tag="IW664_002584"
     CDS             <1..936
                     /locus_tag="IW664_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0849900.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IW664_002585"
     CDS             939..3122
                     /locus_tag="IW664_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0849901.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IW664_002586"
     CDS             3119..4120
                     /locus_tag="IW664_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0849902.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IW664_002587"
     CDS             4135..5049
                     /locus_tag="IW664_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0849903.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..>5356
                     /locus_tag="IW664_002588"
     CDS             5294..>5356
                     /locus_tag="IW664_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="regulates the transcription of tet(M) by
                     attenuation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0849904.1"
                     /translation="MILKYPENICMLCMPMVMHKN"
BASE COUNT         1696 a         1035 c         1132 g         1493 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaat
//
LOCUS       DACEKG010000120         5268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEKG010000120 DACEKG010000000
VERSION     DACEKG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.127"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            38..589
                     /locus_tag="IW664_002589"
     CDS             38..589
                     /locus_tag="IW664_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ0849905.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1265..1531
                     /locus_tag="IW664_002590"
     CDS             1265..1531
                     /locus_tag="IW664_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849906.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1589..2101
                     /locus_tag="IW664_002591"
     CDS             1589..2101
                     /locus_tag="IW664_002591"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0849907.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2098..3135
                     /locus_tag="IW664_002592"
     CDS             2098..3135
                     /locus_tag="IW664_002592"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0849908.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3295..3471
                     /locus_tag="IW664_002593"
                     /pseudo
     CDS             3295..3471
                     /locus_tag="IW664_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            3526..4836
                     /locus_tag="IW664_002594"
                     /pseudo
     CDS             3526..4836
                     /locus_tag="IW664_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
     gene            complement(4966..>5268)
                     /locus_tag="IW664_002595"
     CDS             complement(4966..>5268)
                     /locus_tag="IW664_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0849909.1"
                     /translation="REAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         1984 a          768 c          999 g         1517 t
ORIGIN      
        1 gagcgagcaa gacaggaacg agagaacaaa catactcatg aaaagactag aggcttcgac
       61 ctcgaacttt aataataagt tagatgtttt tgaagaaaag actaagcatg caagattaaa
      121 ctttgaaaca actgcaaagc actatattaa acgacttgat gaagataatt tgaaattaga
      181 ttttcaacaa gctattcaag atgaattaag cgatactaaa gacgaaatac gtgaagtgac
      241 gaaacaagcg agagaagaaa caaaagagta taaagaaatc ttagaaagta aaataaaaga
      301 ccataacaaa gtggttgata aatcgaatac agcgctaaaa gtgatgacaa aaggcgttac
      361 aaatatattt tttgtgctaa ttatttttgt acttgtaatg cttgtgacag gtccgatagg
      421 tcatttcttt ggtattgaac atttatatag ctttattaat ggttttattg atgaccacga
      481 aagtgcatgg cgatatttga tgttgatact ttatgcagta ccctatgtat tcttcgctat
      541 cattctgtgg ctcatagcat tgttttttag agtatttaat gacctttagg tagatttata
      601 tataaaaagg gctaaatacg caaaataccc ccttaaaatg caaaataaat gcaaataagg
      661 gggtaactcc ttatgaaaaa acaactaata gtattataac acgtttttat gccgagaaaa
      721 cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt tggacttgaa
      781 ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt aggttccgac
      841 caataaggaa attggaataa agcaacgaaa ggagttgagt aaaatgagtg agaaaacgaa
      901 gaaaatcatt cgagtatcat tagctttttc acaagtagta gtgcaatcat tgatagttta
      961 ttatacttac cagcaatata aattatctaa agagaattaa gttttgatta tgtaataagt
     1021 tttggtacta aaaaagactt gatctaatta gaccaagtct tttgttagtg ttatattaat
     1081 aacaaaataa aaataagaag tcgctcactc cccgaccaaa gtttgtgagc aaaataaaca
     1141 gtcgatgttt gtttcttaat tgtatatctt gatattaaac gatttaagat tattcttcaa
     1201 gatatatatt cgcaaatgta attggaaaag cgacttctta aacaaaatta aggagtcgct
     1261 ttttatggca aaaataacga aaaatgagca agaaaatttg gtggaaaatt acacaaaaaa
     1321 accggcgtac tctaatagcc ggttagacgc acatttcgtg tgcacttcta atccgaaatt
     1381 gagctttgac gctatgacga tagtcggaaa ccttaataaa aacagcgcta aaaagctatc
     1441 cgattttatg agtttagatc cacaaattag actttgggat atacttcaaa caaaatttaa
     1501 agctaaagca ctacaagacc ccctatgtta ggctagttgt cgtataggat aataagttat
     1561 aataaagact atacggagga tgattaccat gacaaataaa aacaatgaac gttactctac
     1621 cgaactgaaa gcgacagcca tcaagaaaat gatgccaccc gaaaataggg ggattcgcca
     1681 attaagtcaa actttgggca ttccagaagc gactctttgt tcttggcgta aaaaagctag
     1741 aattcaaggg aatactacac ccggaacgaa aaaaaatgtc gaaaaatggt caagtgaaga
     1801 taaattttta attgtgatgg aatcattctc aatgaaccaa gtagaacttg cggaatattg
     1861 ccgaaagaag ggcttattta aagaacaaat tgaggcttgg aagaaaacgt gcttatctgc
     1921 gaatgaacag gaagaaaatc gaacgagaga acttgcaact gaactaaaag aagagaaaaa
     1981 acaagcgcgt caattagaga aagaattacg tcataaggaa aaagcattag cggaagccgc
     2041 tgctctctta ttactaagaa aaaaagcaca cgcgatttgg ggggaccaag aggaagaatg
     2101 atttcatctt cagatcgcaa acaagctgtt gaacttattc aagaagccaa tcgcaatggg
     2161 gcccgtctca cctatgcctg taatgagctg aatattagcg ttcgaaccta cgaacgatgg
     2221 acgcgtgaag gtacgatagc gcatgatcaa cgccctttgg cgaaacgtcc tgtacccaaa
     2281 aataaactga cggatcaaga acgcgaaaaa ataatagaaa ccgtttcaaa aaaagaattt
     2341 atgaatttgc cacctagcca aattgtacca aaacttgcgg attgctcgat ttatattgca
     2401 tcagagtcta gcttctatcg tgtattgcga gaaaaaaata tgcaacatca ccgtggccga
     2461 agtcaagttt cacaaaaacg cataccgcca agtcaccttg cgacgaagcc aaatgaagtt
     2521 tggacttggg atattacttg gctaaaaggt ccaataaaag gactctttta ccggttatat
     2581 ctcatcattg atttatttag ccgtaaaatg gtaggctggg agatttggga atcagaagaa
     2641 gctcgtcatg ccgaaaaact tattaaacag acggtgctta aggaacgtat ccaaggacag
     2701 ccgttggttt tacactccga taatggcagc cctatgaaag ccgcgacttt tcaagtctta
     2761 ctcgaaaaat taggaattca aagttcttat tcaagaccac gagtgagtaa tgacaatcct
     2821 tattcggaag cggctttccg aacgttaaaa tatcgaccag aatatcccaa taacggcttt
     2881 aaaacaatag atgatgctcg aaaatggacg aaagagtttg tcgattggta taacgattct
     2941 catttgcata gtgggattca atttgtgtct cctgcagcgt gtcatgaagg acgacatctc
     3001 gctattttag agaaacgaaa gcaggtttac gaggacgcta aaaagcgcca tcctgaacgc
     3061 tggtcaaaag gtgttcgaaa ttggactcca catcaatcgg tggcgttgaa tcctttaaaa
     3121 gaaacagaaa gttaacagga gcataatctt tcaagattat caaataaccc tcgtcaccca
     3181 ataggggaaa aagttattct tcccctattg gataacgagg ccaaccgacc atagggaggt
     3241 tctgcttttt gataggtgga aaataaaaat acgacaacta tattgacaaa caccgaccat
     3301 cgtccagtaa agagacgcaa taaattctat cgaagtttac gcactgcctc tacaacgatt
     3361 aaaggcatgg aagccatccg aggattatat aagaaaaccc gaaaagaagg cactctcttc
     3421 gggttttcgg tctgtactga aatcaaggta ttattgggaa tcccagctta aatcatagat
     3481 accgtaaggg attttattct ttatttaaaa ctttgcaaca gaaccgaatc attaaaaaaa
     3541 cattatacag agccttggga agatgaagtt tttagagtaa ttattgaata taacaatgtt
     3601 cctattggat atggacaaat atataaaatg tatgatgagt tatatactga ttatcattat
     3661 ccaaaaactg atgagatagt ctatggtatg gatcaattta taggagagcc aaattattgg
     3721 agtaaaggaa ttggtacaag atatattaaa ttgatttttg aatttttgaa aaaagaaaga
     3781 aatgctaatg cagttatttt agaccctcat aaaaataatc caagagcaat aagggcatac
     3841 caaaaatctg gttttagaat tattgaagat ttgccagaac atgaattaca cgagggcaaa
     3901 aaagaagatt gttatttaat ggaatataga tatgatgata atgccacaaa tgttaaggca
     3961 atgaaatatt taattgagca ttactttgat aatttcaaag tagatagtat tgaaataatc
     4021 ggtagtggtt atgatagtgt ggcatattta gttaataatg aatacatttt taaaacaaaa
     4081 tttagtacta ataagaaaaa aggttatgca aaagaaaaag caatatataa ttttttaaat
     4141 acaaatttag aaactaatgt aaaaattcct aatattgaat attcgtatat tagtgatgaa
     4201 ttatctatac taggttataa agaaattaaa ggaacttttt taacaccaga aatttattct
     4261 actatgtcag aagaagaaca aaatttgtta aaacgagata ttgccagttt tttaagacaa
     4321 atgcacggtt tagattatac agatattagt gaatgtacta ttgataataa acaaaatgta
     4381 ttagaagagt atatattgtt gcgtgaaact atttataatg atttaactga tatagaaaaa
     4441 gattatatag aaagttttat ggaaagacta aatgcaacaa cagtttttga gggtaaaaag
     4501 tgtttatgcc ataatgattt tagttgtaat catctattgt tagatggcaa taatagatta
     4561 actggaataa ttgattttgg agattctgga attatagatg aatattgtga ttttatatac
     4621 ttacttgaag atagtgaaga agaaatagga acaaattttg gagaagatat attaagaatg
     4681 tatggaaata tagatattga gaaagcaaaa gaatatcaag atatagttga agaatattat
     4741 cctattgaaa ctattgttta tggaattaaa aatattaaac aggaatttat cgaaaatggt
     4801 agaaaagaaa tttataaaag gacttataaa gattgattat ataatatatg aaaagctatt
     4861 ataaaagaca ttagtattaa atagtttaaa aaaatgaaaa ataataaagg aagtgagtca
     4921 agtccagact cctgtgtaaa atgctataca atgtttttac catttctact tatcaaaatt
     4981 gatgtatttt cttgaagaat aaatccattc atcatgtagg tccataagaa cggctccaat
     5041 taagcgattg gctgatgttt gattggggaa gatgcgaata atcttttctc ttctgcgtac
     5101 ttcttgattc agtcgttcaa ttagattggt actctttagt cgattgtggg aatttccttg
     5161 tacggtatat tgaaaggcgt cttcgaatcc atcatccaat gatgcgcaag cttttgaata
     5221 ttttggttga tcgatataat catgaatcaa tcgattttta gcctcacg
//
LOCUS       DACEKG010000121         5120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEKG010000121 DACEKG010000000
VERSION     DACEKG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.128"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..1169)
                     /locus_tag="IW664_002596"
     CDS             complement(207..1169)
                     /locus_tag="IW664_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0849910.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IW664_002597"
     CDS             complement(1171..2610)
                     /locus_tag="IW664_002597"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0849911.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IW664_002598"
     CDS             2795..4741
                     /locus_tag="IW664_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0849912.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5120
                     /locus_tag="IW664_002599"
     CDS             5005..>5120
                     /locus_tag="IW664_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0849913.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         1665 a          855 c          841 g         1759 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat
//
LOCUS       DACEKG010000122         4855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEKG010000122 DACEKG010000000
VERSION     DACEKG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.130"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..3123
                     /locus_tag="IW664_002600"
     CDS             82..3123
                     /locus_tag="IW664_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0849914.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..3897
                     /locus_tag="IW664_002601"
     CDS             3313..3897
                     /locus_tag="IW664_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ0849915.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFISKKAI"
     gene            3894..>4855
                     /locus_tag="IW664_002602"
     CDS             3894..>4855
                     /locus_tag="IW664_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0849916.1"
                     /translation="MNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGS
                     YDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKE
                     AIHIVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAG
                     AVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFA
                     EKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFN"
BASE COUNT         1610 a          839 c         1042 g         1364 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gtaaaaaggc gatttgaatg
     3901 aattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg
     3961 ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg
     4021 gctcctatga ccgtacggtc aagaccgagt acggggaact tcatctccag attccgcgcg
     4081 accgcaacgg cgagttcaag caacagactg ttcctgctta tagacggacg aatgatacct
     4141 tagaggagac cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact
     4201 tgattgagaa aatgtatggg catcactaca cgccccaaac catgtccaat atgactaaat
     4261 catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt tatgccgcta
     4321 tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag gaagccattc
     4381 atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat gcgattgcac
     4441 caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa cgtggcgtga
     4501 aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc gtgcagcggt
     4561 tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat atcagtcaca
     4621 aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg tatcaagcgt
     4681 catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg aaaaccagtt
     4741 atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc tatgatttcc
     4801 cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt aataa
//
LOCUS       DACEKG010000123         4810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEKG010000123 DACEKG010000000
VERSION     DACEKG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.131"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="IW664_002603"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="IW664_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0849917.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3253..3771)
                     /locus_tag="IW664_002604"
     CDS             complement(3253..3771)
                     /locus_tag="IW664_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0849918.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1581 a          786 c          730 g         1713 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg gtactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     3901 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     3961 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     4021 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     4081 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     4141 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     4201 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     4261 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     4321 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     4381 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     4441 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     4501 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     4561 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     4621 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     4681 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     4741 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     4801 cccccactta
//
LOCUS       DACEKG010000124         4728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEKG010000124 DACEKG010000000
VERSION     DACEKG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.133"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            132..1277
                     /gene="tgt"
                     /locus_tag="IW664_002605"
     CDS             132..1277
                     /gene="tgt"
                     /locus_tag="IW664_002605"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0849919.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1374..1754
                     /gene="yajC"
                     /locus_tag="IW664_002606"
     CDS             1374..1754
                     /gene="yajC"
                     /locus_tag="IW664_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0849920.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2059..4656
                     /gene="adhE"
                     /locus_tag="IW664_002607"
                     /gene_synonym="adhC"
     CDS             2059..4656
                     /gene="adhE"
                     /locus_tag="IW664_002607"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0849921.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          835 c         1010 g         1228 t
ORIGIN      
        1 tcataaaaaa aggataaaaa aaagaatatt aaataaaata gaaggaagcc gagggaactt
       61 gcaaaaagca cttgaaacga ttatgatggt gggagttgtc aagatatttt atatagaaag
      121 tagggaaatg aatgacgcaa cctgccattc gctatcgttt aatcaagaaa gaaaaacata
      181 ctggcgcgcg tttaggggaa ttgatcacgc cacacggaac tttccccaca ccaatgttca
      241 tgccagtagg aacattagct acagtaaaaa caatgtcccc agaagagttg aaagaaatgg
      301 gtgctggcgt tattttaagt aatacatatc atttatggct acgccctggt gaagacttag
      361 tagaagaagc tggcggactg cataaattta tgaactggga tcaaccgatt ttaacagatt
      421 caggcggctt tcaagttttt tcattaagtg atatgcggaa tatcgaagaa gaaggcgtgc
      481 atttcaaaaa tcacttgaac ggttcgcgta tgtttttatc accagaaaaa gcaatcaaca
      541 tccaaaataa attgggctct gatatcatga tgagttttga cgaatgtcca ccattcgatg
      601 aaagttacga ctacgtgaaa aaatcagtag aacgtacgtc tcgatgggca gaacgaggat
      661 tgaaagctca tgctaaccca gaccgtcaag gattatttgg aatcatccaa ggagcaggat
      721 ttgaagatct tcgtcgtcaa agcgcgaaag atctagtcgg gatggatttc cctggttact
      781 caatcggcgg gttatctgtc ggggaaccta aatcagaaat gaatcgtgta ttagaattca
      841 ctacaccgtt gatccccgaa aacaaaccac gttacttgat gggtgtcggt gctgctgatt
      901 cattgatcga tggtgttatc cgtggagtag acatgtttga ctgtgtcttg cctactcgaa
      961 tcgctcgaaa tggcacatgt atgacttctc aaggtcgtct agtagtgaaa aatgcgaagt
     1021 atgctcatga tttccgtcca attgatgaga aatgtgattg ttatacatgt aaaaactata
     1081 cacgtgccta tatccgccat ttgatcaaat gtgatgaaac atttgggatt cgtttgactt
     1141 cttaccacaa tttatacttc ttgttgaatc taatgaaaca agtacgtcaa gcaatcatgg
     1201 atgataattt gcttgaattt agagaagcct tttttgaaga atacgggttc aataaagaaa
     1261 acgcaaaaag cttctaaagg aaaatctttt aaaaattaag taaaaaacta gtgttagcag
     1321 catttttctg ctaaagtgtt ttttgtagat acagaattag tgaggtgaaa aaaatgggaa
     1381 cattaccaat gcttgtgatg tttatcgcgc tattagcaat gtggtttttt atgtcacgtt
     1441 cgcaaaagaa acaacagcaa gaacgtcaaa acttgttaga tagcatgaaa ccaggtgatt
     1501 cagtcgtaac gatcggcgga ttacatggag ttgtttcaga aatcgacaat gacaaacgga
     1561 cagttgtttt agactgcgaa gggatcttcc ttgagtacga tcgtgcagca atcaaaacgg
     1621 tcaaaccagg gactgtcgta acaaatgatg caacaactac tgttgaaaca acagaagaac
     1681 cagctgagac agttgaagta aaaactgaaa caacagaaac gctggaagag acagaagaga
     1741 agaaagaaaa ataagtatca aagcttattt aaagacgagg tagggcagaa agatgtccta
     1801 cctcattttt tattcaaaaa agaaagagat aagagtatag gatatattgc tcaatattga
     1861 ataatcgggt gtgtcatata aataataata aataacttta tatatttttc tatacaaacg
     1921 cctataaata aagcaacttt atatttaaaa ataactgtga aaaaaatcac aaaaaagtgt
     1981 ttacatagaa aaataaatat tgtatgatat acatgtgaat gatgtaacaa ataaccatga
     2041 cccattagga ggataatgat ggctaaagta atggaaaaag aaaaaacgaa gacaattgac
     2101 gtacaagcaa tgattgatga tttggcagaa aaagcaaatg cagcattgaa agaaatggaa
     2161 agctttgatc aagaaaaagt ggaccatatc gtgcatgaaa tggcaatggc agctttagat
     2221 caacatatgc cattagcgaa gatggctgta gaggaaacag gccgcggtgt ttacgaagat
     2281 aaagcaatca aaaatatgta tgcgtccgaa tatatttggc ataatatcaa acatgataaa
     2341 acagttggtg taatcaatga agatgcccaa aaaggattga ttgaaattgc tgaaccagtc
     2401 ggtgtggttt gcggagtaac tccaacaacg aatccgactt ccacaacgat tttcaaatca
     2461 atgatcgcgt tgaaaacaag aaatccaatc gtttttgctt ttcatccaag tgcccaaaaa
     2521 tcttctgcag aagcagcacg agtagtacgt gatgctgcta tcgctgccgg agctcctgaa
     2581 aattgtatcc aatggatcga acatccttcg atcgaagcga cttctatgct aatgaaccat
     2641 ccaggtatcg cgatcgtctt agcgacaggt ggagctggaa tggtcaagtc tgcttactct
     2701 acaggaaaac cagctttagg tgtaggtcca ggtaacgtac cagcttatat tgaaaaaaca
     2761 gcaaaaatta aacgtgcagt gaacgatttg atcgtttcga aaacatttga taatggaatg
     2821 atttgtgctt ctgaacaagc tgtgatcgtg gataaagaaa tctatgcagc agtaaaagca
     2881 gaattccaag cacaccaagt ttatatcgta aaaccagatg aattgcaaaa attagaagat
     2941 gcagtgatga acgaaggaaa atatgcggtc aatccatcaa tcgttggaca ttctgcgatg
     3001 gaaatcgcaa aattggcagg aatcagtgta ccaaaaggaa cgaaaatgct gattgctgaa
     3061 ttggaaggcg taggcccaga ttatccgcta tcaagagaaa aactttctcc agttcttgcc
     3121 atgatcaaag ctaacaacac ggaccatgcg tttgatttgt gtgaaggtat gttgaatctt
     3181 ggaggattag gacatacagc agttatccat tcagaaaacg aagagttgca tgtcaaattc
     3241 ggtcttcgaa tgaaagcttg tcgtatttta gtgaatacac catctgcaga aggcggcatc
     3301 ggtgatatct ataacgaaat gattccgtca ttgactttgg gatgcggttc ttatggtaag
     3361 aactctgttt ctcgtaacgt ctctgctgta aacttaatca atgtcaaaac tgtagcgaaa
     3421 cggagaaata acatgcaatg gttcaaatta ccgccaaaaa tcttctttga aaagaactct
     3481 ctattgtact tagagaaaat ggaaaacgtt gaacgtgtca tgatcgtttg tgaccctggt
     3541 atggtccaat tcggttactg cgatacagtt cgtgaagtat tggcacgacg taaaaatgat
     3601 gtgaagatcg aagtcttctc agatgttgaa ccgaacccat cgacaaatac cgtatacgca
     3661 ggaacgaaac tgatggcaga cttcaagcca gatacagtaa tcgctcttgg aggtggttct
     3721 gcaatggacg cagctaaggg aatgtggatg ttctatgaac acccagacac atctttcttt
     3781 ggtgcaaaac aaaaattctt ggatatccgt aaacggacat ataagatcga caaaccagaa
     3841 aaaacacaat tcgtatgtat cccgacaaca tcagggactg gttctgaagt aacgccattt
     3901 gcggtcatca ctgacagtga aacacatgtg aaatatccgt tagcagatta tgcattgacg
     3961 ccagatgtcg caatcgtcga tcctcaattc gtcatgtcag ttccagcatc tgtcacagca
     4021 gataccggga tggatgtgtt aacacatgca atcgaatcct atgtttcagt catggcttca
     4081 gattatacac gtggattgag cctacaagca atcaagttag tctttgacta tttagaaaaa
     4141 tcagtaaaaa cacctgatat ggaatcacgt gaaaaaatgc acaacgcttc tacaatggct
     4201 ggtatggcgt ttgctaacgc attcctaggt atttgtcact cagtcgcaca taaaatcggc
     4261 ggggaatacg ggatccctca tggacggaca aatgcgattc ttttaccgca tattatccgc
     4321 tataatgcaa aagatccaca aaaacatgcg atgttcccta aatatgacta tttccgtgca
     4381 gatacagatt acgcagatat tgcaaaattc ttaggtctga aaggcgaaac aacagaggaa
     4441 ttagtcgaag ctttagcaac ggctgtttat gaattaggaa aatctgtcgg catcaacatg
     4501 agtctaaaag ctcaaggagt cacacaagaa acactagata caaccgtaga ccgtatggct
     4561 gaattagctt acgaagacca atgtacgaca gctaatccga aagaaccatt gatctctgaa
     4621 ttgaaacaga tcatcattga tgcttacaac ggataagcaa aagaaagcgt agaaaaaaga
     4681 taagaaaata gatgaaaaag aacttggaaa acgttgtttc tccaagtt
//
LOCUS       DACEKG010000125         4701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEKG010000125 DACEKG010000000
VERSION     DACEKG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.134"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            544..1107
                     /locus_tag="IW664_002608"
     CDS             544..1107
                     /locus_tag="IW664_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0849922.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IW664_002609"
     CDS             complement(1386..1673)
                     /locus_tag="IW664_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0849923.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IW664_002610"
     CDS             complement(1663..1992)
                     /locus_tag="IW664_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0849924.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IW664_002611"
     CDS             2297..2626
                     /locus_tag="IW664_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0849925.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW664_002612"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IW664_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0849926.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IW664_002613"
     CDS             complement(3551..4462)
                     /locus_tag="IW664_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0849927.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1545 a          800 c          707 g         1649 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc c
//
LOCUS       DACEKG010000126         4571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEKG010000126 DACEKG010000000
VERSION     DACEKG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.135"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(338..1291)
                     /locus_tag="IW664_002614"
     CDS             complement(338..1291)
                     /locus_tag="IW664_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0849928.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1314..2069)
                     /locus_tag="IW664_002615"
     CDS             complement(1314..2069)
                     /locus_tag="IW664_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0849929.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2066..3025)
                     /locus_tag="IW664_002616"
     CDS             complement(2066..3025)
                     /locus_tag="IW664_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0849930.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3018..3968)
                     /locus_tag="IW664_002617"
     CDS             complement(3018..3968)
                     /locus_tag="IW664_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0849931.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4118..4570
                     /locus_tag="IW664_002618"
     CDS             4118..4570
                     /locus_tag="IW664_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0849932.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1490 a          962 c          775 g         1344 t
ORIGIN      
        1 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
       61 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      121 tttgccactc cccattctag aatcaacccg tatgatgtag agaatgaatt cacgttgaaa
      181 aaagatttcc caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      241 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      301 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      361 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      421 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      481 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      541 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      601 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      661 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      721 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      781 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      841 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      901 tttgaaaatc tctttgccgg ccagaaatga tgatcaggtc tggctgaagc tgattgattt
      961 tttctaagtc tggttctttg atcccacctg ctgattccac ttttttatag gatgaaagat
     1021 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1081 tcgtatctaa agaaccattg tcgaatacga cgactttttc aggattcttt ggcacctcga
     1141 tttgatcgcc attactgtct tttatcgtta atgccgtttc agcttgcgta ctgcttttct
     1201 ttgtatctgc ctgactggtg ttgtcactat ttccacatgc tgctaaacca aatgatgcta
     1261 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1321 taaagacaga atctgcgacc ttcaatctca caaattcgta tgtccatttc gtacaattcg
     1381 tccagtacat ctttttttat catttcttgg gtcgttcctt gcgtgaataa ctgcccatct
     1441 ttcattgcaa taatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1501 acaattgttt tcccgatttc ttgtactaat ttttttaaga tctgcatgat ttgaaccgca
     1561 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1621 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1681 tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt
     1741 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1801 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1861 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1921 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     1981 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2041 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2101 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2161 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2221 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2281 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2341 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2401 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2461 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2521 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2581 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2641 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2701 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2761 agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2821 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2881 caaggataaa tgcaaaaatt ttcttactcc tcaatgccca tgcaaaatgc cagtttccat
     2941 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3001 aaatagatgg ctttttttta agcatatcgt cgccccctta tcagaagata aaggaagata
     3061 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3121 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3181 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3241 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3301 cctaaaaact gcatcttctg gtaatctatt cccaaattta ctgcaaacga ttcgcccaga
     3361 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3421 tacaataact cataatttcc tcgtatcaca gtcgcaaaat ttccttgaag ccatgaagtc
     3481 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3541 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctgggggaa caatcgcgta
     3601 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3661 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3721 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3781 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3841 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3901 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     3961 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4021 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4081 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4141 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4201 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4261 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4321 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4381 tagccgattt actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4441 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4501 agaaaactct tcgccagttg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4561 tttattttaa a
//
LOCUS       DACEKG010000127         4430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEKG010000127 DACEKG010000000
VERSION     DACEKG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.136"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW664_002619"
     CDS             complement(543..2048)
                     /locus_tag="IW664_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0849933.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW664_002620"
     CDS             complement(2045..2731)
                     /locus_tag="IW664_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0849934.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW664_002621"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW664_002621"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0849935.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DACEKG010000128         4401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEKG010000128 DACEKG010000000
VERSION     DACEKG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.137"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(99..1619)
                     /locus_tag="IW664_002622"
     CDS             complement(99..1619)
                     /locus_tag="IW664_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0849936.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1715..1840)
                     /locus_tag="IW664_002623"
     CDS             complement(1715..1840)
                     /locus_tag="IW664_002623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0849937.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            complement(1862..2047)
                     /locus_tag="IW664_002624"
     CDS             complement(1862..2047)
                     /locus_tag="IW664_002624"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ0849938.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2031..2213)
                     /locus_tag="IW664_002625"
     CDS             complement(2031..2213)
                     /locus_tag="IW664_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849939.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2454..2672
                     /locus_tag="IW664_002626"
                     /pseudo
     CDS             2454..2672
                     /locus_tag="IW664_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3167..3766
                     /locus_tag="IW664_002627"
     CDS             3167..3766
                     /locus_tag="IW664_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0849940.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3830..>4401
                     /locus_tag="IW664_002628"
     CDS             3830..>4401
                     /locus_tag="IW664_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849941.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         1403 a          799 c          785 g         1414 t
ORIGIN      
        1 ataattcagt agttttgagc gtaagcgtgt catagaatgg tacctaaaca caaatagccc
       61 gatttgatac actcaaatca ggttatttgt ggcttatatt atttacagat ttcttgtatc
      121 tgctgattcc atggcaaata agcgcgtagc gcttcaggtg tcatcgaaga cagattggga
      181 atctctgtaa aaagatgatt taaatatttg cggatgttta acccattcgc aacagctgtc
      241 tggataatac tcaatataat tccatttgat ctagcgcctt ttaagctttt agaaaatagc
      301 cagttttttc gaccgatcac taactctttg atggcacgct ctgctcggtt atttgaaagt
      361 tccaagcgtc catcaagcaa cacaatctga agtccgtctt tatgattgac tgcgtattga
      421 accgcctttc caagctgcga ttttggcaaa taagcaaaac tctcacacca atgaaaaaat
      481 tcagtcatca caggctgcaa cgtttccttc ctcgctacaa acctttcttc tgcgctaagc
      541 ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga agtctaatcc tattttggca
      601 tgactttttg gggacggttg tttacccaat gcttcaaaaa actttctgcg aacatgcgcc
      661 caacaataaa cattgtttac cggcagcttt ccataggccg cgtaaccatc agtatgcaag
      721 aaaccgctaa atgattctaa aaaacttttt ggctcatcaa cggaacgcga atttgcgtgc
      781 tcgtagagca caacttgttt ttcttcgctt ttacctgaag catataacca gtaaaaggtc
      841 ttttctttat gagtattcaa tacccgatag cttgtctcat ccgcgtgtaa gacctcctga
      901 ttgcttaatt cttgatgaag gcaattgtag attggctcta aaaccatttc acttaccttg
      961 atatgccaat tagtgatttt tttacgatca agagggaaac caagtgcatg ccattctttt
     1021 tcttgtcgat atgccggcac ctttttaaca aatttttccc aaatactctg tgtcacaatt
     1081 gaagaggaac ctagactatt attgatcaac ggtttaggaa caggcgcttt tttgattttt
     1141 gtttctccta atgcttcaca ttctctgcaa tgataggcat gttggtagat gacttgtttt
     1201 tctatatggg caggaacata caatggctct tctctaagcc ttgtcgcacc gatatcatgt
     1261 aaagtatgac cacagtcttc acacaaacaa tcggcacctt ttaattcata atgaatagga
     1321 acttcaggca gaccttctaa tgaaagtgct ttgagtccct tgcgttttct gcgtttaaaa
     1381 gaagataccg taatgggttc ctggctttct tgtccagtgt gctctggttc attaaaagtt
     1441 gagtcgtcta ataaatcgat ttgcccatct acgggtggtg tttctttttt tcgtccaaac
     1501 aagcgtcgat tcatttcagc aatttgttct ctaagaagag tattttcctg ttgctggatt
     1561 tcaagcaact gtgttaattg cttgatttgt acaagaagtt cagattccat ttttgtcatt
     1621 tttacaccac cattctagcg attttcttac atctattata ccattttatg acaacgaaaa
     1681 acagacaaaa tccttgattt aacaaggttt tcgtctaata tatcgcacct tttttcgttt
     1741 cttttagcgt acatttttct tcgattgaaa ggccagtcag taagacttct tttctggaac
     1801 gcggccattg aaggtaccca ctttcaaatc ttttgtacat taatacaaat ccattttgat
     1861 cctagtaaag agccttgaag cgatctttct tcgtaccgca aaataagaaa agcgcttgat
     1921 cgtaaacatc aaggtcatat tcttctataa cgaaagctgc tagtccatca attccttttc
     1981 gaagatctgt ttttccacaa acgataaaaa tatgtttcaa tgcagttgca tcaatgagca
     2041 ttggcattca actcttttaa caaggtatac aaaagatatt tatccattcc attataaata
     2101 aatatttcct tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc
     2161 gatttttgag ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt
     2221 tctccttcta tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta
     2281 tcctatgaca cgcttacatt taatttaata ttttgcctca aattagccac actttttata
     2341 gaactaagag gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata
     2401 tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca
     2461 tttactcaac taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca
     2521 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
     2581 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
     2641 aacaaatgct gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt
     2701 gacacaaaat tattgacgct cccaactaaa agtgtcaaaa taatgatgaa atcatttagt
     2761 tcttataaat tagaaaagag aaaataaata ttccatatct actttattgg atccgtaaca
     2821 tgactaaatt ctgaatactt tttaaagctt atcatttggt aaaactgaat ataagttaat
     2881 tatatagact gaaaattttg aattttcaga atagaataac ttaaaagtac tgacaaaatt
     2941 ttttaaaggg gcaatattaa tgttctgaga taatcaaatc ctaccgaaaa tatcaaaaat
     3001 ccaatgtgac acaatcagat tgtgtctcat tcgagggaat cacacaatcc cacgtggctc
     3061 taggcagaat tgaatttttg cttggggctt tttccttata ttgattttga atgagactta
     3121 ttatatgtta taatgcaatt aagtatcatt ggagagggcg aagaaaatga agacggtaga
     3181 acctattcga gacaaaaaaa agatcgatgc gatgaaggca atcttggctt cgggaaaata
     3241 tggccaacga aatttggttc ttttttcgat tgggatcaac accgcctatc gcatttctga
     3301 tttgcgtcaa ttgaagctgg ccgacgtgct ggaaatttca cgcggtcgcg tggtggtcaa
     3361 agatcgatta gcgatgaagg aacaaaaaac agctaaacat aattcggtct atatttccaa
     3421 caaattgcga aaaacgattt tggagtacgt tcaacaggaa tttcctgatc aactccttca
     3481 gcaagatttt gacggttatt tgttccctag tcgaaaaggg acgaatgaac cgctcaaccg
     3541 ccaaagtctg tggcgaatca ttcatgatgc tggcacggca atcggattga aagacattgg
     3601 cccacattcc atgcgaaaaa cgttcggtta ctttctctat aaacagggga ccaaaacaga
     3661 aatcattcag tccttgttga accattcttc tcagcgcgaa acattgcggt atattggtat
     3721 tacacaagaa gacaaagata cagctgtaaa aagcttagat ttataatata aaagttaagt
     3781 ttaaacatgg atagttttaa attagtagat tgagtgttgg gaggaaagaa tgagtacatt
     3841 atatattata ggaaacggtt ttgatttaag tcatggttta aaaactagat acactgattt
     3901 ttcaaaatgg gttgaaaaaa gtcatagtga aatattcgat aaaatagaca caatgataaa
     3961 tgaaaataat gaggtgagaa gtacagctga tgataagata ggttggttta attttgaaag
     4021 tagtttgcat gagttaattc aaagtccagt ctttgaagat tatgtagaag agtctaagga
     4081 ttatctcata ggttatggac atgaagattg gtcagattct gcccatcatg attttcaaaa
     4141 tatggtttat gattatgttg tttctctaga agaattacaa caacttttca aggaatggat
     4201 acgcaaagaa atacagccga caatagaaca taaaattcca aaataccaat tttcgtccga
     4261 tgtactattc tttacattca attatacaga tgttttggaa attctataca agcaaccgaa
     4321 agaaaacata tatcatattc atggcagtta tgacgatata ataatcggcc actccgagaa
     4381 ttttgacaac tttataggag a
//
LOCUS       DACEKG010000129         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEKG010000129 DACEKG010000000
VERSION     DACEKG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.138"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1051)
                     /locus_tag="IW664_002629"
     CDS             complement(83..1051)
                     /locus_tag="IW664_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0849942.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IW664_002630"
     CDS             complement(1498..2904)
                     /locus_tag="IW664_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0849943.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IW664_002631"
     CDS             complement(3126..3488)
                     /locus_tag="IW664_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849944.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IW664_002632"
     CDS             complement(3492..3800)
                     /locus_tag="IW664_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849945.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IW664_002633"
     CDS             complement(3809..4228)
                     /locus_tag="IW664_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849946.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IW664_002634"
     CDS             complement(4221..4361)
                     /locus_tag="IW664_002634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849947.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEKG010000130         4372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEKG010000130 DACEKG010000000
VERSION     DACEKG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.139"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            159..1082
                     /locus_tag="IW664_002635"
     CDS             159..1082
                     /locus_tag="IW664_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0849948.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1100..2341
                     /locus_tag="IW664_002636"
     CDS             1100..2341
                     /locus_tag="IW664_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0849949.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            2475..3821
                     /gene="gor"
                     /locus_tag="IW664_002637"
     CDS             2475..3821
                     /gene="gor"
                     /locus_tag="IW664_002637"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0849950.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1504 a          690 c          941 g         1237 t
ORIGIN      
        1 atttttgtca caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt
       61 ttgtgaaata attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga
      121 gctatgctac tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag
      181 tagaaactat tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg
      241 gtgaagagca atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat
      301 tggtagaatt agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc
      361 atcctgaaaa aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag
      421 atgtgatcac agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc
      481 gtataccgta tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct
      541 gggcgaaaaa aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga
      601 aggcgatgga agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca
      661 tccatcaatc caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac
      721 aaaaagatcg tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt
      781 ctatttatga agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga
      841 atgaattgaa tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta
      901 ctccaggcga aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt
      961 tagaaggtag ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc
     1021 atcctgaaaa ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat
     1081 aaggagtatg atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc
     1141 gccagcagga acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata
     1201 tattggcggg aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat
     1261 ggctgaagga gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat
     1321 ggtcacccat gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt
     1381 cggtatttct gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc
     1441 cccaggtctg ccgatccatt tatccactca agcttcagca actaattttg agacattgga
     1501 attctggaaa gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga
     1561 agtggcagag attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat
     1621 gtgtatctct tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa
     1681 ccgtggcgga tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg
     1741 tcaagaacga aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc
     1801 agtggatatg tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt
     1861 caagatcgaa ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa
     1921 agcggctgta gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat
     1981 cgacgagtta tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc
     2041 aactgaaaac gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg
     2101 tgaagtatta tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt
     2161 ttctgtagga gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt
     2221 tgaagttatg cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat
     2281 tttaactatg ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata
     2341 aggaaaaagc tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg
     2401 ttacacttgt tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa
     2461 taaggaggaa caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta
     2521 ttgcttcagc aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg
     2581 aattgggcgg aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa
     2641 gtacgatttt ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa
     2701 aaaatgttga tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg
     2761 gtgcttatca aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt
     2821 ttatcgatag tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga
     2881 tcgctactgg aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt
     2941 caaatggatt ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat
     3001 acattgcggc tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat
     3061 atcgaaaaga acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa
     3121 tgtaccaaga agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa
     3181 acaatgacga atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac
     3241 tttttgccgg tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg
     3301 aattggacaa aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata
     3361 tttttgctgt tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg
     3421 gtagacgttt atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac
     3481 ttgttccgac agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag
     3541 aagcattgga aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa
     3601 tgtactttgc attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa
     3661 aagaagaaag aatcgtcggc ttacatgcta ttggtgttgg tgtcgatgag atgctgcaag
     3721 gttttgccgt ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca
     3781 tccatccaac aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca
     3841 acagcttttt attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt
     3901 ttttgttttt tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag
     3961 ggaagttgga gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta
     4021 tgcactctta agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag
     4081 aacagatgtt taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg
     4141 ttaaaagaat accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga
     4201 atcaaatcaa gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc
     4261 cgaacaaata gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg
     4321 aggaactatt tttccatttg tttctctaat actcaggaca aaacactgat tt
//
LOCUS       DACEKG010000131         4368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEKG010000131 DACEKG010000000
VERSION     DACEKG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.140"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..530)
                     /locus_tag="IW664_002638"
     CDS             complement(27..530)
                     /locus_tag="IW664_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0849951.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(653..1417)
                     /locus_tag="IW664_002639"
     CDS             complement(653..1417)
                     /locus_tag="IW664_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0849952.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1525..1800
                     /locus_tag="IW664_002640"
     CDS             1525..1800
                     /locus_tag="IW664_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0849953.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2238..3188
                     /gene="yidC"
                     /locus_tag="IW664_002641"
     CDS             2238..3188
                     /gene="yidC"
                     /locus_tag="IW664_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0849954.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(3367..3567)
                     /locus_tag="IW664_002642"
     CDS             complement(3367..3567)
                     /locus_tag="IW664_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849955.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         1302 a          920 c          769 g         1377 t
ORIGIN      
        1 atatggtcta ttattggttt tatctattaa atcgaggatt ctttcccaaa gtaacgttgc
       61 catctcttac gcatacttgg tgacataggc tgagcggctt ctttgactgc gcagtatttg
      121 tctacgaatg ttacgatata tccttctttg tacttaggcg gtgcaatcgt tgcgccccac
      181 atgtgcttca aaatgatatc tcgctcaaga tccgacaatt cggtgatctt ttctgcattc
      241 tttacagcaa ttcttggatg gatatacgca tgtgtgcctt catcaaattt cgtcgtacgc
      301 caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca
      361 ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga
      421 gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga
      481 tcttctacat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
      541 ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc
      601 ggattattct agtcgccttg gataaaatag ccaaagctta atagttcctt aatcataaag
      661 aaaaatgata aagaacaatc cctgccgcaa ccccaacatt caacgattcc gcatttcctt
      721 tcatcgggat atatacgttc tgatcagtca aagacaagat ttcctgactt accccctgac
      781 cttcgttccc catgattatt gcgaagttat ctgatttttc tactttgtct aatgcgtgtg
      841 cttcttcatt taattctgtg ccatacactg taaacccttt gtctttcatt aaaggaatca
      901 cttctgtcaa atctttttgc aacacaggca aatgataatt gcttccctgc atgctgcgca
      961 gcacctttgt actataaata tctgccgttc ctttgcctaa taaaacacca gccatatcaa
     1021 atgcatctgc cgtacggatc atcgtcccta catttccagg atcttgtaca ttatctaaca
     1081 acagccatcc tcctgaaaaa tctggcgtgc cttcagccgt taacgccatc actgctaaga
     1141 ttccttgtgg tgtaggtaga tccgatacag catccatgac ctcatccgaa actagaaaag
     1201 tagaaatctc tttgtttgcg gccagccagt ctccccattc tttcatgcct cgctgtgtca
     1261 caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcaatcaga tggaaccctt
     1321 ccaaaagata ttcttccttt tcttcccgat atttcttttt atgtagtttt tttaattctt
     1381 tgattcttgg attttttgct gattggatct ctttcacatt cctcacctcc ccatgattat
     1441 atcatggaat aggtgcttga gcttgtttca tcctaaaaaa atcggtatca ttaatttata
     1501 aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa tgaacgtcca aggacgcgtc
     1561 caaggcgtag gatttcgcta catgaccaag atggtcgctg atcaactagg cgtgactgga
     1621 acggtaaaaa acgaagatga cggctcagtc acgatcgaag caatcggcaa tgacgacatc
     1681 atccagaaat ttattgaaga agtcaagaaa tctccgagcc caagtggacg agtgcagtat
     1741 gtggatgtcc aagaagaccc gatgattgag gaacgaaaga aatttgatgt ggtgggataa
     1801 ggttgtgaaa aagctggtct gcatcaagca ttaaggacag gttgtgaaaa atagctctcc
     1861 atatttttca acaggctgga cttaatgccg tagatgcaag cttttgaaca ccgtcgataa
     1921 ggttattctt gaggcgcttt gcctcaaaaa ttaaggacag cagtgaaaaa gctgatctat
     1981 cacttgcagt gagttcattt gactccgctt tttcgggcag aaaaaaagtt tttagtttat
     2041 taaagtaaga aagaaacact tggaatcatt ctactttttc ctagtatttc tttcttgatt
     2101 ttttgtcttt ttttcaaaat ttttcgctac gtaaaagaac gaatatttat atgaaatttc
     2161 tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg taaaaattat
     2221 agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc ggacttgtca
     2281 gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac cctgacactt
     2341 cgggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg
     2401 aaaatttcaa ttggtcttat ggctgggcag ttattgcaat gactgtgatc gtccgtatca
     2461 tcatcttgcc actaggtatt agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc
     2521 aagcgttaaa accacaagtt gaagcagccc aacaaaaatt gaaagctgca acaacacgtg
     2581 aagaacaaat ggcagcacaa gcagaaatgc agcaagtata ccgcgaaaac ggattgagta
     2641 tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt
     2701 actttacggc acgttataca gaaggtatcc gggaatcatc tttctacggc atcgatttag
     2761 gctcaccaag tatggtactt gttgccattg caggggtcgc ttatctgctt caaggataca
     2821 tctcaacgat cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct
     2881 ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca ctttactggg
     2941 tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg atgaaaccac
     3001 gtttgaaagc acaagtggca gaagagttga aacaaaaccc accaaaacaa gtcgtcactc
     3061 caagaattga tgtgacagaa tcagctaaac cagctgacag caaacaagca accaaacaaa
     3121 tcaagcaacc caaaaacagt acaaacaata aaggccgcaa cgcaggaaag cagcagaagc
     3181 ggaagtagat agtaaataag agtgtaataa aaattcatta acagttttag caattaaaaa
     3241 tatcgaccaa gatttttgaa aaagaaatct ggtcgatatt tttatttaag tgaatgttta
     3301 atttgaatca tcgattgatc gctatctaat tgcagcagca tctttttatg aagaaacaac
     3361 tttttcttat ctatcaacgt agacaggtgt tgctgtttct tctaatagtg tacttaattc
     3421 cttaatattt tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt
     3481 aacaaaaggc gccactccct tcgcccatgt tgctcgccca cataagacac cattctccat
     3541 aaaggacaac gcggagatac cacccatcta aataacatcc ataaaccttt ctagtagaag
     3601 aatagtgata gtatccatcc ctttttctac tgggaataca attacacgat tcaaagcttg
     3661 atacacaaca tttcccttct tataaaccgc tattgtaacc gaattgtttt gaatcacctt
     3721 tttgggatag aaactggctc ctttccgcaa cttcttaaat gttggtttct tttatacttt
     3781 ctttgtagca actacaaatt tcttcaaatc tctaaatgtt tgcataacaa ccgcctttct
     3841 gacttttact caggatggta tttaaaaatc agcaaaataa ggcttctctc catctttttt
     3901 atttcctagc tgctttaaag acactcttgc tgttttttaa tctctatcaa gggggctctg
     3961 aatctatttt gatgaactta tcttattaat ttctcaaatg agctgtcaga cacaactgta
     4021 ttttttcaat ctcataccgg acaactatgc cagcttaaaa aaatttcctc tggcaaaact
     4081 tctttattga aaaatgacaa tgaaaaaaga ccattctttt tgttgaatgc tcattgtgta
     4141 aacatatttc ctgcgactgc tcttcgaacc attctcagtg caatccctaa tttatctatt
     4201 aaatcttctt ttagttcctc atctttcata ttcaaaattt cctttgtatg ttacacaagt
     4261 ttgttctcta aataacgcga attgtaaaat taagttagaa aaataaaaag gcatttatgg
     4321 tacactcaaa ttgtatttcc gacgaaagaa aacaaaggag tgtaacca
//
LOCUS       DACEKG010000132         4215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEKG010000132 DACEKG010000000
VERSION     DACEKG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.141"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            624..3263
                     /locus_tag="IW664_002643"
     CDS             624..3263
                     /locus_tag="IW664_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0849956.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="IW664_002644"
     CDS             complement(3431..4129)
                     /locus_tag="IW664_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0849957.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          805 c          911 g         1221 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgataagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagcatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttat
//
LOCUS       DACEKG010000133         4200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEKG010000133 DACEKG010000000
VERSION     DACEKG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.142"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1223)
                     /locus_tag="IW664_002645"
     CDS             complement(3..1223)
                     /locus_tag="IW664_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0849958.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1848)
                     /locus_tag="IW664_002646"
     CDS             complement(1243..1848)
                     /locus_tag="IW664_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0849959.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="IW664_002647"
     CDS             complement(1897..2829)
                     /locus_tag="IW664_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0849960.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="IW664_002648"
     CDS             complement(3254..4111)
                     /locus_tag="IW664_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0849961.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1468 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaagat
//
LOCUS       DACEKG010000134         4176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEKG010000134 DACEKG010000000
VERSION     DACEKG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.143"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            612..1424
                     /locus_tag="IW664_002649"
     CDS             612..1424
                     /locus_tag="IW664_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849962.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3059..3613
                     /locus_tag="IW664_002650"
     CDS             3059..3613
                     /locus_tag="IW664_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0849963.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3867..>4176
                     /locus_tag="IW664_002651"
     CDS             3867..>4176
                     /locus_tag="IW664_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849964.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         1543 a          516 c          661 g         1456 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
     2041 tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     2101 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     2161 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     2221 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     2281 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     2341 aagaactagt agaagaagga ataatggctt gataataaag tactacctat taaaggtagt
     2401 actttattta atttagtata aattaacata ctactatatt ttaagtttca tattatataa
     2461 attaataata tactaccatt ttttggtagt ataatttaaa ggtaataagt tattctttta
     2521 ttactatgtt tgggttttat agcaaggaaa gtttaataag agtttggacc tagcaaacaa
     2581 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
     2641 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
     2701 cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg ggagttctta
     2761 gtatattgtc caaatttaaa tttcatataa tttaattatg taaattagct attctaagaa
     2821 cttctacgga taactgcccg tctaaaattt aattaagagc ttagaatctt catataagta
     2881 aggttaattt ttttgtacaa aatagtcgga atattctgag ttactgaaat gatataaaat
     2941 cataaactaa accccttgat tttcttcact tttaacagag tatatatata agttcatgat
     3001 tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt
     3061 gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct
     3121 aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg
     3181 gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc
     3241 tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga
     3301 agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca
     3361 acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa
     3421 attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta
     3481 caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat
     3541 tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa
     3601 attgggaggc taattgagat attaggatat attttttgtg tataatgaaa acttagaagg
     3661 tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca
     3721 atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg
     3781 attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa
     3841 taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt
     3901 taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt
     3961 taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa
     4021 catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc
     4081 aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa
     4141 aaatttttgc cagcattcat gattatatag gtttga
//
LOCUS       DACEKG010000135         4136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEKG010000135 DACEKG010000000
VERSION     DACEKG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.144"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..156
                     /locus_tag="IW664_002652"
     CDS             <1..156
                     /locus_tag="IW664_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0849965.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IW664_002653"
     CDS             175..1452
                     /locus_tag="IW664_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0849966.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IW664_002654"
     CDS             1462..2118
                     /locus_tag="IW664_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0849967.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IW664_002655"
     CDS             2118..3494
                     /locus_tag="IW664_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0849968.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3780..>4136)
                     /locus_tag="IW664_002656"
     CDS             complement(3780..>4136)
                     /locus_tag="IW664_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAQ0849969.1"
                     /translation="EKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKL
                     FQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITV
                     SSIVMFLWLFRMGVIP"
BASE COUNT         1645 a          464 c          724 g         1303 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     3961 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact
     4021 tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag ccaggtggct
     4081 aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac tttttc
//
LOCUS       DACEKG010000136         4052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEKG010000136 DACEKG010000000
VERSION     DACEKG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.145"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..212)
                     /locus_tag="IW664_002657"
     CDS             complement(<1..212)
                     /locus_tag="IW664_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0849970.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIP"
     gene            complement(1049..2134)
                     /locus_tag="IW664_002658"
     CDS             complement(1049..2134)
                     /locus_tag="IW664_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0849971.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(2454..2861)
                     /locus_tag="IW664_002659"
     CDS             complement(2454..2861)
                     /locus_tag="IW664_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849972.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(2880..3698)
                     /locus_tag="IW664_002660"
     CDS             complement(2880..3698)
                     /locus_tag="IW664_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0849973.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT         1240 a          649 c          563 g         1600 t
ORIGIN      
        1 gggataggat cttttaaaat tatttgtttt tgtttcccaa tgtaataaat catttcttct
       61 gagactttgt ctttctggat tatatatttc ttacactgca caacggtttt actttgacca
      121 gttgatagtt tcttcgtgat cttttctgta gcggtacacc gtttaccgta gtgacaaata
      181 tcttcgagac tcaaaattcc tgtatatgac ataaaataat ccacccactt actttaaata
      241 tttttaacaa gtttttcttg ttatttttca aaaatagaga attgattttc gttcatgaca
      301 aacaaaaaag agggaacgca ttcaaacatg cgttccctct aatttcagcg ttcgtcatac
      361 ggcaaagagc cccacggaca tttttataac attatgctta ggcatagatt catcgtaata
      421 tcacgtattt ttaaccttaa cacagccaaa aaaagtggta tcacttttga atatctgtta
      481 tactaaatcc gttacgggta ataccgtaac actgaaaggt aaacgcatta aggggtatag
      541 acttaatgcc acctgtggac gtcgacgaag gttggtagcc ttccgagtct cacagggcct
      601 ttcttttttt tgtattttca attgtccaaa tttatcgtcc aaaagtgcag aacgaaaatc
      661 aattcatcac aagcatagca gacaattaaa tttaaaacaa ctaaatttat acaaaaaagt
      721 gtaattgaat attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggt
      781 aaataagtga attacctctc ttcatgaatt attataactc aaaaaaatac atatgcaaac
      841 actagttccc tgtttttttg aatgaaaaat aactaaaata tctaacgatt atgacattaa
      901 aacctacgtt tgtgattgtt ttcataaact tttaatctaa aaactccgga ataataacta
      961 tttatttgta acatttgtaa taaaattaaa attgtaaaaa agttctaatt taaaattgat
     1021 catttttata gtgttcgctt tttatcaact actctagcca attttccgta ctaactgttg
     1081 gtaattcgtt aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcaa
     1141 taaaccagtt ttttacatac acaaaaatca aattctgcaa attatttact ttctcagtct
     1201 tttgtttttg ataagctttc agcaacgtca tatataatcc tttatccgca tcaattccgt
     1261 aagattcaag ttcttcatac acaactaccg tcttctcaag ttcctcggct ttatgttttg
     1321 ctttgtgtat tgtggtgacg gtttgttggg cttcttcgta agtgttagaa aatgttttta
     1381 tcaagcttaa taccttttct ggtacaaatg tgtttatgct gtcttctttc atgatttcaa
     1441 cgaagttatc tagcaaaact tgattttgta attggtctgt ttcagtgtct tttaagtgtc
     1501 ttttagtgtc taaagattta ttaatataat aatcttgtgc gatttccgca tttccagtgt
     1561 tatcaagggt ttcagctgtt ttttctcgac gtgcgatttc cgcatttcca gtgttatcaa
     1621 gggttttagc agtttcctct cgacgtgcga tttccgcatt ccgagaagag gttgatttat
     1681 caatagtttc gcttaaatcg tcatcgaaag aattttgaat aaaaagatca tcggcactta
     1741 tttcaggttg aagtaaatag tatttatttt tttcattttt tttcgttttt gggttaaatt
     1801 ctccttttac gactgaaatc aaattatatg tttctaactc gtttaaaatg gtttttactt
     1861 tgccttggta gcaatttaat ttgtccatta ggtctgcttg tgtaaattcc aaatataata
     1921 gaccatcatc gtcgatccat ttttcatgta aggctctacg aaattcattt ttaaaaagca
     1981 tataggctat ttttgagctg tctttcattt tagagtattt agaattctga attaaaaaac
     2041 taggaaattt ataatatttt tcaatataaa cattcgttgc tttatatccc caatttttaa
     2101 ttatcatttt ttgagattga ttttctgtag acattaaatt tctccttctt cttagaaact
     2161 tgtatatatt caggattttc aacaagtgaa ctataccatg tattaagggt gaataaaaca
     2221 ctttcagtat ttaaggtata agttatatat taaaaaaata agtataagtt atactttaaa
     2281 tacaaataat gtataactta tactttaaat aataatttta taatgttgat ttaatcgcaa
     2341 atttacacgt tgtttattaa gttatacctt aaatatttaa tgtataactt atactttaat
     2401 attcatttat ctagtataac ttatacttta attgaatacc gcatgaaaaa aaattatttt
     2461 ggtaattttt caaactcttc ttgtatttct tctcttatgt gttcttcttt tgctactcct
     2521 agtctatgct ggatatatga gtctgctaat gtattaacaa ttttttcgaa agaaattctc
     2581 cctttttcac tttgtaactc cgtcatatag tcttgtaaaa ttgagagttt gctatttaat
     2641 gccggagaga tctttttagg aactgatacc ttgtttcttt tatcaggaat tgaaactggt
     2701 cggcctcttt ttttcaatgt aatatttctt gtgttactca atgcactatt gctaaagtta
     2761 tattcaccgc ttattttcac atcttggcgt ttgttattgg gtaataatgg ctcaaattca
     2821 ccattctcca tcattttagg aatatcaaaa tctttaggca ttacttatgc aactccttat
     2881 tatttattta aaaactcttg tctagcatct agttctgcca aaatatttat aaatacttgg
     2941 tgtgctttat aatcccattg atcaaaattt ccatttttgt attttcttaa atgtatacca
     3001 attttagaat accttctcaa tcttttttga taatgaatga gatttttgga taccacggtt
     3061 ccgtattttt caattgcttc ttctaggatt tcttcatcaa aatcatcatt tggttctacc
     3121 atacaaggta caatatctat aatttgcaaa tcaagatcgt atctatcgac catgaatttt
     3181 tgataattta catatttgtt gactccctct aaggattctt cagctgtttg aaatgaaatt
     3241 atcgaatagt ctgaagctgc catagcatta tcggaaaatt cgctaatggt tggtggtaca
     3301 tcaataaata tttcttgata attgttcttt attggaagaa gtaatttttt caagacagta
     3361 acttggtcta attcattttc atgtttatta gcaaaagcgg agaatgatcg aaaactagtg
     3421 tcacatgcca gaaggtcaag attttcattt accttaataa tactacttgc taattctcca
     3481 tttgcgacac catttgttat agtggttttg atatcggtta taccgaaagt tcttgctatg
     3541 acttgtgtta agttcgcttg agggtctaaa tcaataataa gcgtttttct atttcttatc
     3601 attgcattgt cataagcaag catgctgact atcttagttt tacctactcc tcctttaaaa
     3661 ttgccaactg taaatgtagt cccttgaaaa gtatccatgt aaatcctccc aatctttaat
     3721 tgataattaa agtataactt atatatcgga aatggtcaac attaaaagta taacttaaat
     3781 aataatatct aaaaacgttt ggatatagcg gtttttaata ataaaagtat aagttatacc
     3841 atatatattt atggtataac ttatactttt attattaaag gtataactta taccttaaat
     3901 attgcttttc gaaaatagct attctagtct atctgtttct ttacttttcg ctgctgattt
     3961 gaattgaatt gatcatattc attttctagt attaataact tttcgtatac atcggtcata
     4021 ttcatactag aagcaagact tagatcatat ac
//
LOCUS       DACEKG010000137         4035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEKG010000137 DACEKG010000000
VERSION     DACEKG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.146"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            178..774
                     /locus_tag="IW664_002661"
     CDS             178..774
                     /locus_tag="IW664_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0849974.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IW664_002662"
     CDS             898..2697
                     /locus_tag="IW664_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0849975.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="IW664_002663"
     CDS             complement(2975..3187)
                     /locus_tag="IW664_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849976.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1185 a          688 c          712 g         1450 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat attta
//
LOCUS       DACEKG010000138         4015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEKG010000138 DACEKG010000000
VERSION     DACEKG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.147"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(176..1462)
                     /locus_tag="IW664_002664"
     CDS             complement(176..1462)
                     /locus_tag="IW664_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0849977.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IW664_002665"
     CDS             complement(1455..2723)
                     /locus_tag="IW664_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0849978.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IW664_002666"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IW664_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0849979.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IW664_002667"
     CDS             complement(3495..3941)
                     /locus_tag="IW664_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0849980.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1137 a          816 c          707 g         1355 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaat
//
LOCUS       DACEKG010000139         3947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEKG010000139 DACEKG010000000
VERSION     DACEKG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.148"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(595..1152)
                     /locus_tag="IW664_002668"
     CDS             complement(595..1152)
                     /locus_tag="IW664_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849981.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1331..1942)
                     /locus_tag="IW664_002669"
     CDS             complement(1331..1942)
                     /locus_tag="IW664_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849982.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2055..2312)
                     /locus_tag="IW664_002670"
     CDS             complement(2055..2312)
                     /locus_tag="IW664_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849983.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2909..3187)
                     /locus_tag="IW664_002671"
     CDS             complement(2909..3187)
                     /locus_tag="IW664_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849984.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3221..3472)
                     /locus_tag="IW664_002672"
     CDS             complement(3221..3472)
                     /locus_tag="IW664_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849985.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1199 a          809 c          523 g         1416 t
ORIGIN      
        1 aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc ttgcatctgc
       61 cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat aatttattca
      121 tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt tctcaatttc
      181 ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca ctttttaagt
      241 tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt tagttgatta
      301 gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt agcttatacc
      361 tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg tatttgccac
      421 tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg ctctcttttt
      481 tattcagatt catttttgct gatcacagcc tagtatttca atggttttga acactaaacc
      541 aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt tcgattattc
      601 aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc caaacctatc
      661 gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt tattgcgtgg
      721 atattctatt gttacgccat taaatggata ccaattagga ttaggctctc tttttttctg
      781 tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc gaccatcctc
      841 taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta gacgccacgt
      901 gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg caacaattgt
      961 atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat caaactcccc
     1021 tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta aaaccgcatt
     1081 cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt ttctatttga
     1141 taaatttctc attattcgca catcacctct acattctcac atattagttc gtaatactcc
     1201 tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct actactcgta
     1261 taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt ctatttatcg
     1321 taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc cagttgatta
     1381 aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt acagttctta
     1441 tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc tcgagatttc
     1501 taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata taatattgcg
     1561 cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct tctctaccac
     1621 tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg aactcacacc
     1681 cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct agttcgtatt
     1741 caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta ggtgagatta
     1801 atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct atgatcacaa
     1861 ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg tattccattt
     1921 tattccccct attactattc atatccgcct atctatatcg atcttccaat gcagagataa
     1981 ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt ctccactaag
     2041 gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac tcctccaaaa
     2101 acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt atatgtgttt
     2161 gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat cgcattcgta
     2221 tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat cattgtgatt
     2281 tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta tagaaatcgc
     2341 ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg tctccatttt
     2401 tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca caacacgtta
     2461 ccttttaaat cattatactg tatctcatac attattatca ataaactatt ataaaaatgc
     2521 aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa tcataccctg
     2581 tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg caagacagac
     2641 actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt acccactttt
     2701 gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac cccaagaaat
     2761 taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca acatttaaat
     2821 tttttatgct gttcggtctc agaaatgccg tttttgctta tatcacaata tcaagatgtc
     2881 aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta ttaagactaa
     2941 tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat gatcctgcaa
     3001 gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc tttttcccat
     3061 taggatcttt tattttatga attttctgca ggtaatctct cctaatggca gctattttta
     3121 cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt atgttgtgtt
     3181 tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa agttgaatga
     3241 gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca tatcctcccg
     3301 caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa gagaaaagag
     3361 tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag ataacaactc
     3421 caaacattga atttttatac aatgaccatt tatttatagg cttttctttc attttacttc
     3481 cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc tctttgaaca
     3541 tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt tataggcgag
     3601 ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact gaaattaaag
     3661 cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac atgtctttac
     3721 ttttgaaatt ctatgatgaa aagccttgga atacagtagc tcttaattgg taatactttt
     3781 tccattataa ataacctctt ttcattttat atttatcccc tctaaaaata atatctaatc
     3841 taatatttta aacttatatt tccctcgctg tgctaataga gcattactaa ataaatagaa
     3901 actagaaaaa agcattacgc ctgccagaat aaaccaatta tttgtat
//
LOCUS       DACEKG010000140         3810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEKG010000140 DACEKG010000000
VERSION     DACEKG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.149"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..2193
                     /locus_tag="IW664_002673"
     CDS             94..2193
                     /locus_tag="IW664_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0849986.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2389..3252
                     /locus_tag="IW664_002674"
     CDS             2389..3252
                     /locus_tag="IW664_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0849987.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3357..>3810
                     /locus_tag="IW664_002675"
     CDS             3357..>3810
                     /locus_tag="IW664_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0849988.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1524 a          636 c          772 g          878 t
ORIGIN      
        1 agtaaatcct aaaaataagc tattggcgtg acgtatagaa tatgatagat taaatggtag
       61 cgtaaaccta gtaaaggagc cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg
      121 aaaaaaatgg agcagaatac tatctgtcca gactgcggtc atcaaataac tgcagaagat
      181 cagcaaataa aagaatcaat tgaagaaaca aatacagatg cagctatgca ggataaatca
      241 actgaagatg cagtctttcc tgatacagaa ttaaacgatc caatcgaatg gtcggaattg
      301 aaagatttac ctctagaatc tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa
      361 gaaccacctg ctcacgatca agtaactaat atagagaaaa atgaacagaa gaaagcaact
      421 gttcatgaga cttcggaaga atcaggaaag aatcaagaag aaacgcttga atcaaaacaa
      481 aaaagaagag tagctgaact gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta
      541 tccgcttata tcaaagcaca tcgtgaagat acaaaagaag agcacgcgaa agagctgctc
      601 aaaatgatca gtgaaaaaat tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa
      661 actgaaaaag actctgatag agaaaacgcc acaggtaaag aaacagtaaa aataaaagac
      721 gtaaaaacgg atatcacaga aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa
      781 aaaacgaata aactgccgga agcagagaaa aaagaaatac tcgaaaacac agacaaaaag
      841 tcaacagagc ccgatgaaat aaatagtgaa tccgatgctg aaaatcaatc tgtaagcaaa
      901 gagggtactg atcttgaagc aaaaatagaa aaagaagaga accagcctaa gctgtttgaa
      961 gcagaagcga aatcaataac aatcgtgcca gatactgaaa aaacacatca agaatcaaac
     1021 ttagaaaaaa cagatccaga taaagcgcaa aagaaaaaat caaaaaaagt accttatctc
     1081 gttttagcag cagtacttct actcggtgga ggaagctggg cttattatga ccatcatcaa
     1141 aaagtagaag ccgaagtagc agcagaagca aaaagacagc aacaaatgga agaacttcat
     1201 gagaaacttg ctgcctttta tacagatgat caccatcaat ttattcgtgc agatatgatt
     1261 catcaagatt tatcaaaact aaaaaatgat ttatccagtc ttaagaatga aaaaggatat
     1321 tcagagttag aaacaactta ccaagatatc caatctaaga tccgagatat ccaaaaagtg
     1381 aatgagtggt tcatagcacc tgccatcgtc gatgatcatc tagcagatga catcaagttg
     1441 aaagcagata aagagatcca agcaatcgaa acggataaca cagattttgg aaaattgatt
     1501 caacaagcac aaacagaagc taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa
     1561 acagctatag tctttaaaga cggaaaagta gtggattcag caacacgcga ccaatacaag
     1621 acggcaaaag aagcagtaga aaaagtgaaa aatacagatc tatctgctca gttgaatgaa
     1681 caattgaaac aagtagataa gatgctcact gataaagaga aaaaagaaga agaggaaaag
     1741 aaaaaagcag aagaagcgaa aaaagcagct gaagaacaag caaaacaagc cgctcaagca
     1801 gcagctgcag ctcaagccgc tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg
     1861 gcatcctcga ccccttcctc ttcaaactca gcgaatcagc caatcaaatc tgccagagca
     1921 agtgatattg ccgatgcatc taatccagca tggacgtggg caccaggtgt ccaaagcaaa
     1981 gtgattgcta catgtatcca acgtggatat atcgttgaag gaggttacag attagaaaaa
     2041 gccagaatcg aaaatgggga aggctattat aatctatatg ccacttcaac aaaatctgca
     2101 ttgatgaaag gcattggaga aagtgctttg ccattttaca tcgtaaccat caactgtaaa
     2161 actggatggt tcggaggaaa tgggagcaat taattttagg caaatgaaag aggctgtgag
     2221 cctgccgttt aacaccgttt attcgaaaaa ggggagtatg acacaactgt tgtcatactc
     2281 cttttttcac ctttccaaac caaggctcag ctattcaagt agataacatt atgttatgat
     2341 agcaaagaaa tgtattaaga aaacctttca ctatagaggg agaaaaaaat gaacgattta
     2401 ttagcaacta tatttacagg tattattatt gatgaaaatg aaaaaaacta ctttgtccaa
     2461 aaaaacggac aaacctttgg cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca
     2521 gttgaaggat tcggttacca aaatcaaaaa catgaaaatc ggatcactac acaaattcct
     2581 aaaagccgta tcggtcatta cgcttttgga atcgttactg ctacacgaaa agatttaggt
     2641 gcgtttgtcg atatcggtct tccagataaa gactttgtcg tatctcttga tgaattgccg
     2701 acaatgacag agctatggcc taaaaaaggg gatcgcttga tgatttccct tagagttgat
     2761 gcaaaagacc gtatttgggg cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa
     2821 tatggaacag aagaaatgaa aaacaaagat gtgataggaa cagcataccg cttgaaatta
     2881 acaggtacgt acgttttaac ggaagatttc tatcttgggt ttatccatcc ttctgaacga
     2941 tataatgaac ctcgtctagg cgagcagata aaaggtcgag tgattggtgt ccgaccagac
     3001 ggtgtactga atctttcatt gaagccaaga gcatatgaag ctatatcaga tgatgcacaa
     3061 atgattttaa ccattttaga acgttctgca gatcagcaaa tcaactttac agataaatcg
     3121 gatccagaag aaatcatgaa agcttttggt atcagcaaag gagcatttaa gcgcgcatta
     3181 ggtaatcttt tgaaacaagg attgattaca caagaaaacg gtgtgacaaa actagtcaaa
     3241 aaatctaatt gagaatccct tgcatgtttt tcttaactag cttataataa ttttaattaa
     3301 catagtgtta tatctttttt tatttgtaat tattataaat tgttggggtt gagtaaatgg
     3361 tcatgaatgc tatttcggcg atgaaaaaaa caaaacagca gctacatgaa gcggggttta
     3421 agctgacacc gcaaagagaa gctactctgc ttgtgctttt ggaaaatgag aaagagcatt
     3481 tatctgcaga agaaatcttc tttttagtga aaaaaaagag tccggaaatt ggacttgcta
     3541 ctgtttatcg aacccttgaa atcttgacag aattgaaaat tgtcgataaa gtaagtttta
     3601 atgatggatt agcccgttat gatttgagaa aagaaggcgc caaacatttc catcatcatt
     3661 tactatgctt gaactgcggc acgatcgagg aagtagaaga ggatctttta cttgaagtgg
     3721 agcaagtagt agaagaacgt tatcattttt tagtaaaaga tcatcggcta acgttccatg
     3781 gaatctgtca ggaatgctat gaaaaagaaa
//
LOCUS       DACEKG010000141         3681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEKG010000141 DACEKG010000000
VERSION     DACEKG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.150"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..277
                     /locus_tag="IW664_002676"
     CDS             <1..277
                     /locus_tag="IW664_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ0849989.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="IW664_002677"
     CDS             420..641
                     /locus_tag="IW664_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0849990.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IW664_002678"
     CDS             657..1499
                     /locus_tag="IW664_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849991.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IW664_002679"
     CDS             1562..3364
                     /locus_tag="IW664_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ0849992.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(3423..>3681)
                     /locus_tag="IW664_002680"
     CDS             complement(3423..>3681)
                     /locus_tag="IW664_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0849993.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSLLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1312 a          523 c          749 g         1097 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactgctgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aacaaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaatggata tagtaatgcc c
//
LOCUS       DACEKG010000142         3680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEKG010000142 DACEKG010000000
VERSION     DACEKG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.151"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2277)
                     /locus_tag="IW664_002681"
     CDS             complement(<1..2277)
                     /locus_tag="IW664_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849994.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLA"
     gene            complement(2949..3217)
                     /locus_tag="IW664_002682"
                     /pseudo
     CDS             complement(2949..3217)
                     /locus_tag="IW664_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1057 a          801 c          566 g         1256 t
ORIGIN      
        1 tgctaaaccg tcaattgcca gttttccatc ttctcctgta gttaaaccgg tttgtagcgt
       61 ttctccttct cggttctgta actcaaaaac agctccttga aggacttcac cactgccttc
      121 agcatgttta attagttcaa caccacccgt atcaaggatg ttttcaatgg ttaacgtcac
      181 ttcttctatg gagtattcat ttataaaaac atcatgtacc gtcgcatcta aaagataccc
      241 ttccggtgct tctgtttctc ggacttgata gctacctaca ggaagcggtt cacttaaaaa
      301 ttctccgttt tctgccgtta taccttcttg tatcacttgt gaatcgcttg ataaaatttc
      361 aaattttgct cctgccagcc tttggccatt ctcatcagtt ttaattactt taatacgccc
      421 atcgattttc cttgttctta cagtactcgt attcaatcga ttgtcactat tcacaagcgt
      481 tgcaaagttt aaaatttgat cgccgaccgc atacgcctct acattgacct taaaagagag
      541 ggtaaaactt gtcggaccac caatcgtatt attcgcactg aaacgaacta ttccattatt
      601 gacttcggca tcaatgaaag agggaatatc tgatagaaat gacacaaatt ccatgccttc
      661 ttccaaaaca tcttcaataa caagcgtatt tcttgttcca gaaaacatcg agacatgcag
      721 agtatacgtt agttcatttt cattgagatt gataacattt ttgtccacac tttttcttaa
      781 agagattgcc ggaagattac tttcagggat tctcaagcgg tagtctcggc tcattacctg
      841 attattgtag gtcatactcg ctcgattccg agaaaaccca tcctcttgtc ttccttcatt
      901 gaacaagtcg acatcggtca cttcaagagc atattcaata aaatacgatt gaccctctgc
      961 tagattacca aagtccacaa caagtgtatt cgtttcctta ttaaaagata ctttgtccac
     1021 aaattcctcg gaaacataat gagttctaac gtttgcttca cctggattcg ttacttctgc
     1081 gatccgaacc ccacctgaaa taggttctct atttccagtg gaactccatg tagtcggatt
     1141 cggtgccaaa gttgttcctt gcggtagtac atcggtaacc actacaccgt cttttcgtga
     1201 tccttcataa tttacctgca aaataaattt attactgttg gtcacgtctt gcccgtcaat
     1261 cagcagattc ccaagatttc cttggctgtc gatttctcta gagccatagt ataatttcga
     1321 aaaaatgggt cttgatgaag gaataggcgt aattgaaaat tccgtgtttg ctgctcgctc
     1381 agcataagta agactcactt gaaagtcatt atttggctca gtaccgcctg attgaaagaa
     1441 agcaacttca aacgtgatac tgaatgtata cgactgagcg gcttcattta atccttcata
     1501 atcaaaggta aatggaaaaa caacgtctgt gccttcttgg taaaatgcca gtggctttaa
     1561 aggtgaatca tcagcgacct ccactctaat gaactcaatt aagccaatcg atgctctagg
     1621 aactcgtacg accatctccc catattcttc aagaatatcg ccagcgcctc cttgaatgta
     1681 tccctttaac gtttgtgtat gcctgctcgg caaagtgggg cgctcagcag agatgccgat
     1741 gtcataattc aagagctcct cttgtctttc aatctgagat tcctgcgtta tagatggctc
     1801 agatgatctg gcttcattaa aagattcact taagctttct tctggtatat taaaagattc
     1861 acttgtgctt tcttctggta cattaaaaga ctcacttgtg ctttcttctg gtacactgat
     1921 ttctattgag accgtatttg attgaatatc accgcttctt gcggtcaatt cgtatttacc
     1981 tgaatgctgg gaagaataag taatcagacg ttcgtaatca ttgagttgct tcacttcccc
     2041 ttcaatcaaa tcctcactct tatcaatgac caatgcattt agcaggtctg gtgttcctct
     2101 gactcgcaac tggaactgct cacccaaagc agcctcctct tgatcaactt cgatcgtcag
     2161 cgaacgctct ggctgttctg ctaaagactc agctagaacc gttatgttcc caaattgatt
     2221 gatcacaata agcataacta gaaacatact taacagttta tgaaatttgt tcctcactat
     2281 tactccccct tataattttg actaaacaac aaggtacccg ataattttct aaaaagattc
     2341 cccaactcaa aatggctgcg ttacctccct cgtattttcg gttcaactca agcgttgcaa
     2401 tgtaagctgt aaacaaagct aatgattgcc gattgtcttc tgttgattga ttcgatagct
     2461 ttgtatgact tttagttgtc cgtgtggctt aaccataatt atgatttcac gattcctcct
     2521 gccagtgcct tgcacaagct ggagcgtaac cctaatagtt attttgttaa tgtacttata
     2581 tacttttata cttttccatt agaaacaatc actaaatcgt tgataccaag gtcagactct
     2641 agaatttcat cagtgagtcc ctgtattggt taaccttcat caatcccaaa aaaatatatc
     2701 aatgcttagc atcaaaatga atatatagtt tagaatactt gggattcatc gataactgat
     2761 cgcttatttg gccatattat ttggcacttg gttcaactaa acgcatttag gatgttcctt
     2821 cattctcgtc atggatgttc tcaaaccgtc attactaata aactgtccac cgaatcgatg
     2881 gttcatttct tgcattttcc acgtccaatt actctttttg ctctttattt gggcgtagtt
     2941 tcactttctt aacttgtatc gtcttccatc aactccttaa tcatcttctt agctttattg
     3001 gctcggtttc cttgtaaaag gtatctacaa atagtgacaa tctgtattaa atcttcatca
     3061 ttttctgtaa tgtcaataat ttctatattc tgtaccatat attgcaaagg gtttcactaa
     3121 gttcataacc gaagcgaatt aaacaacttt tgtatagaat aactaccttt tctatttttc
     3181 cattggtaat tctatctatt aattggttta atccttttgt tgtagttgat tccactacca
     3241 agatgattct ctcgaatttt aaagcttgaa gatgtattgc tatttttgct aactggcgta
     3301 tttgcaacta tttttccctt ctattcttcc ttcattgatt cccctctaaa attactatcc
     3361 ataaaaaatt gcataaatga caatagtatt tatattagtc atactgcttt ttaacacagc
     3421 aaattccaga aaaacattcg ttacatattt gtgaattttt ccaaaaaata acatttttat
     3481 aaaacgacga aaaataaatc actaccagtc aacttctata atattaccaa ataacaaact
     3541 aactatccat caataacgtg ttttattttt taaaatttct ataaaaaaac aatcattctt
     3601 aaaaagaata tataaaaata acaataacct ttgtgatttt tcttgtttga agaatgttag
     3661 aagaaaaatc tctaaatact
//
LOCUS       DACEKG010000143         3460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEKG010000143 DACEKG010000000
VERSION     DACEKG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.152"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(862..1203)
                     /locus_tag="IW664_002683"
     CDS             complement(862..1203)
                     /locus_tag="IW664_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0849995.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IW664_002684"
     CDS             complement(1203..1433)
                     /locus_tag="IW664_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0849996.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIGDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IW664_002685"
     CDS             1720..2058
                     /locus_tag="IW664_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0849997.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IW664_002686"
     CDS             complement(2263..2502)
                     /locus_tag="IW664_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0849998.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IW664_002687"
     CDS             complement(2478..2825)
                     /locus_tag="IW664_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0849999.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1089 a          647 c          537 g         1187 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct ccaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3241 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     3301 acctctccac taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     3361 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     3421 caacctctaa tacacggtgt tccagaagta gcttcttcga
//
LOCUS       DACEKG010000144         3402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEKG010000144 DACEKG010000000
VERSION     DACEKG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.153"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1073)
                     /locus_tag="IW664_002688"
     CDS             complement(75..1073)
                     /locus_tag="IW664_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0850000.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="IW664_002689"
     CDS             complement(1148..1444)
                     /locus_tag="IW664_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0850001.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="IW664_002690"
     CDS             complement(1437..1730)
                     /locus_tag="IW664_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850002.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="IW664_002691"
     CDS             complement(1876..2427)
                     /locus_tag="IW664_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ0850003.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="IW664_002692"
     CDS             complement(2601..2900)
                     /locus_tag="IW664_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ0850004.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3193)
                     /locus_tag="IW664_002693"
     CDS             complement(2927..3193)
                     /locus_tag="IW664_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0850005.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
BASE COUNT          994 a          668 c          460 g         1280 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcaca
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3241 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa at
//
LOCUS       DACEKG010000145         3396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEKG010000145 DACEKG010000000
VERSION     DACEKG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.154"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            647..964
                     /locus_tag="IW664_002694"
     CDS             647..964
                     /locus_tag="IW664_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0850006.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IW664_002695"
     CDS             complement(1055..1420)
                     /locus_tag="IW664_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850007.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IW664_002696"
     CDS             1628..2110
                     /locus_tag="IW664_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850008.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IW664_002697"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IW664_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0850009.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1086 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttt
//
LOCUS       DACEKG010000146         3374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEKG010000146 DACEKG010000000
VERSION     DACEKG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.155"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IW664_002698"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IW664_002698"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0850010.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IW664_002699"
     CDS             complement(2261..2512)
                     /locus_tag="IW664_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0850011.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IW664_002700"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IW664_002700"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0850012.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          740 c          626 g         1088 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttt
//
LOCUS       DACEKG010000147         3351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEKG010000147 DACEKG010000000
VERSION     DACEKG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.156"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..238
                     /locus_tag="IW664_002701"
     CDS             <1..238
                     /locus_tag="IW664_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850013.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="IW664_002702"
     CDS             252..440
                     /locus_tag="IW664_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850014.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="IW664_002703"
     CDS             452..1063
                     /locus_tag="IW664_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ0850015.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="IW664_002704"
     CDS             1048..2439
                     /locus_tag="IW664_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0850016.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            2655..2957
                     /locus_tag="IW664_002705"
     CDS             2655..2957
                     /locus_tag="IW664_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850017.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..3297
                     /locus_tag="IW664_002706"
     CDS             2950..3297
                     /locus_tag="IW664_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850018.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
BASE COUNT         1307 a          473 c          655 g          916 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 gaagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa aggctcttcg tcaaatcgtg ttggtgaaga
     3301 caaatttgaa taaaacttag tccgatatgc ggcgcgttct gtgcgccgca t
//
LOCUS       DACEKG010000148         3311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEKG010000148 DACEKG010000000
VERSION     DACEKG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.157"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            260..1214
                     /locus_tag="IW664_002707"
                     /pseudo
     CDS             260..1214
                     /locus_tag="IW664_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1231..2255
                     /locus_tag="IW664_002708"
                     /pseudo
     CDS             1231..2255
                     /locus_tag="IW664_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2261..2740
                     /locus_tag="IW664_002709"
     CDS             2261..2740
                     /locus_tag="IW664_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0850019.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2756..>3311
                     /locus_tag="IW664_002710"
     CDS             2756..>3311
                     /locus_tag="IW664_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0850020.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         1008 a          552 c          762 g          989 t
ORIGIN      
        1 ttcgttttca taaatctatt ataaaagaaa agactgtaga catcatccat aaaatggact
       61 ttgtctacag tctggaactt ggaaaacgtt gtttctccaa gttctttttt taatttccaa
      121 gtaactgaaa gttacttttg ttatttaaca tttaggtcaa ttttagacag tttttgttca
      181 aaaagcgata tttatagtgg acggtattac tgatatatta aggatataaa atgaaagcgt
      241 ttaaaaaggt ggcgtaaaga tgattattaa agaagagatt tttgtaccta aagaattagt
      301 gaaagaaaaa attgacctgc tcgttgaaaa cctgacaacg atcaaagacg ataatggtga
      361 atttttgttg ggctttgatg gactgaaagt agatgacaag agctggacag tgtggaattg
      421 gccacaaggt gtcggtctat atggtattta taaaaattac cagatgacaa aaaatcctcg
      481 cgcttatcaa gtggtcaatg aatggtttga ggatcgtatg gaagaaggcg ctccgcccaa
      541 aaacgtcaat acaatggcac cgctattgac tatggcttac ttgtatgaag acacgaaaga
      601 ttcacgctat ttgccttatt tagaacaatg ggcagaatgg gtcatgaatg acatgcctcg
      661 gacaaatgaa gacgggttac agcatgccac atatggaccg gaaaataaga atcagttgtg
      721 ggacgataca ttgatgatga cagtgcttcc tttggcaaaa atcgggaaac tgctggttga
      781 cgattgctat tccagagatc attgaaatct tagaattatc gaaagaagat gcattgcgca
      841 aattattgat cgaaacactt gaagcacaag tgcgtgcatt aaaaacttat cagtctgaaa
      901 gcggtttatg gcatacgttg ttggatgatc cttcttctta cgtagaatct tctgcaactg
      961 caggatttgc ttacggtatt ttgaaagcgg tccataaacg ttatttacca caagaatata
     1021 aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca aatcgatgaa aaaggagaag
     1081 ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt agatttttat cggaatatcg
     1141 gaatcacagc aatgccttat ggacagtcgt taacggtgct ttgtttgaca gaattgctcg
     1201 tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa catcactgct gtttgcagca
     1261 agttcgctta gcctaggtct gctaggtcta agcggctgcg gaaataattc tagtgtggca
     1321 actgtcgcag atgcagacgt aaatgagata gttaccttgc gctttgctta tgcaagcaac
     1381 agccagtaat tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt
     1441 ccgtacagat ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat
     1501 tgacacagtc aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct
     1561 ctaaagatta ttcgattttt tccgttcctt atctattcaa tgatgaagaa catttcttta
     1621 aggtgatgga taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat
     1681 ttaccggatt gacctattat gattctggtc agctcagctt ttatatggta gacggtccga
     1741 tccatacgcc ggaagatttg aagggaaaga agatccgtgt gatgcaaagc gaaaccgcta
     1801 tcaaaatggt agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca
     1861 cgtctcttca atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag
     1921 ctggacatgg cggggtagct aaatactatt cctatgatga gcatacacgt gtgccagata
     1981 tcatcatcat gaatgatgcc atcaaagaac agctaactga tgaacaggaa aaagccattg
     2041 aagaagcagc gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag
     2101 aagaaaaagc tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac
     2161 cgtttcgaga ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag
     2221 acctttatca aaagatccaa gagcaaggag cgtagctgta atgaaaatga ttttaaacaa
     2281 ggtattagag atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca
     2341 agtattttcc agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt
     2401 cggtcttgtc tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacgttt
     2461 agccgtaacg gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt
     2521 tgttcaaagt ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc
     2581 tgtcacattg acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta
     2641 tctgtcagta cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga
     2701 tacattaagc aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc
     2761 aatagaagcc ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc
     2821 gattgctata agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga
     2881 tgtttctgct tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat
     2941 cgctattccg tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa
     3001 actagtggat tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa
     3061 tgtgttaggg aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc
     3121 gattggtgga gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac
     3181 agcggtcaat atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat
     3241 catgtattca cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct
     3301 cgtaggcgct t
//
LOCUS       DACEKG010000149         3295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEKG010000149 DACEKG010000000
VERSION     DACEKG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.158"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            135..1103
                     /locus_tag="IW664_002711"
     CDS             135..1103
                     /locus_tag="IW664_002711"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0850021.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1420..1944)
                     /locus_tag="IW664_002712"
     CDS             complement(1420..1944)
                     /locus_tag="IW664_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0850022.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2231..>3295
                     /gene="scm"
                     /locus_tag="IW664_002713"
     CDS             2231..>3295
                     /gene="scm"
                     /locus_tag="IW664_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0850023.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1049 a          667 c          611 g          968 t
ORIGIN      
        1 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
       61 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
      121 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
      181 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
      241 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
      301 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
      361 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
      421 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
      481 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
      541 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
      601 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
      661 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
      721 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
      781 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
      841 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
      901 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
      961 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
     1021 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
     1081 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
     1141 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
     1201 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
     1261 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
     1321 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
     1381 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
     1441 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
     1501 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
     1561 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
     1621 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
     1681 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
     1741 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
     1801 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
     1861 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
     1921 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
     1981 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
     2041 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
     2101 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
     2161 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
     2221 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
     2281 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
     2341 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
     2401 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
     2461 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
     2521 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
     2581 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
     2641 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
     2701 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
     2761 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
     2821 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
     2881 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
     2941 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
     3001 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
     3061 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
     3121 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
     3181 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
     3241 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DACEKG010000150         3257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEKG010000150 DACEKG010000000
VERSION     DACEKG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.159"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(67..2559)
                     /locus_tag="IW664_002714"
     CDS             complement(67..2559)
                     /locus_tag="IW664_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0850024.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2566..3033)
                     /locus_tag="IW664_002715"
     CDS             complement(2566..3033)
                     /locus_tag="IW664_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0850025.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3169..3242)
                     /locus_tag="IW664_002716"
     tRNA            complement(3169..3242)
                     /locus_tag="IW664_002716"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3206..3208),aa:Arg,seq:acg)
BASE COUNT          868 a          721 c          536 g         1132 t
ORIGIN      
        1 aaatcttgtc acaacctctt aatcaagtct tactattata aaaattcagc tacacaggcc
       61 aagtaactat agggaaccta ctgtttcctt tttaggtccg cgaacattca atgtgatctt
      121 accttttgaa gcacctactg ttacgtggtc acctaaacga atttctccgc caagcaaagc
      181 ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga cggattggac gtgcaccata
      241 ttcaggatca aatccagctt tcccgatcac atcgattgca gatggtgtga ttttcacttg
      301 aatatcttgt tctttcaatc gtttgataat cgctttgctc atgatttgga cgattgtatg
      361 gatttcatct ttactcaatg aatggaagac gacagtctca tcgattcgat tcaagaattc
      421 aggtcggaaa gctttcttta actcttctaa gatacgtttt tgcatggctt tgtgatcttt
      481 agtaacatct tggacattga atccgacatt tttttcttct cgaatttgtg tcgctccgat
      541 atttgaagtc ataatcatga ttgtatttct aaagtccact tttctacctt tagaatctgt
      601 taaatgacca tcatccaata cttgcagaag taaattgaaa acgtctggat gtgctttctc
      661 cacttcatct aagagaatca ctgaatatgg tttttgacga attttttctg tcaactgtcc
      721 accttcatca taccctacgt atcctggagg agacccgatt aatcggcttg tactgtattt
      781 ttccataaat tctgacatat caacacggat cagggcatct tcacttccaa acatgacttc
      841 tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt ggtccaagga acatgaatga
      901 accaattgga cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg cacgtgaaac
      961 tgctttgaca gcctcatctt gaccaacgac acgttcatgc agaagtcctt ccagttctaa
     1021 caagcgttcg ctttcttttt tctccaactg ctgtagaggg acaccagtcc actccgatac
     1081 aactgtagca acatcttctt ctgttacacg atcagaataa ccttgctcct gttttgcttc
     1141 aattgcagaa actttttgca gctttttacg tagtgctttt tcttgtctgc gtaattgagc
     1201 agcattttca aagtcttgtt tttggatcgc tgcttccttt tcttgtacta gttcatcgat
     1261 ttctagtttg atcacagttg attttgttaa gtgatcggtt tgatccaaac ggactttcgc
     1321 tgctgattca tcgatcaaat caattgcttt atcgggtagt tgacgagaag tgatataacg
     1381 aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg atctccactc catggtgctc
     1441 ttcatatctt gtacgtaatc cttgaagaat cacttctgct tcttcaggag ttggctcatc
     1501 tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat atttttgata
     1561 ttcatctagg gtcgttgctc cgatcgtttg taattctcct cgagctaacg ccggtttcaa
     1621 gatattcgaa gcatcaattg ctccttctgc tccacctgca ccaattaagg tatgcaattc
     1681 atcaatgaat aagattactt gaccatcatt atagatttcg tcgatgacct ttttcattcg
     1741 atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa gatcccatat caagcatcat
     1801 caggcgcttt tcttgcatgt cttctggcac ttcacctaaa atgatttttt gagctaaccc
     1861 ttcagcaatg gctgttttgc ctacaccagg ttcacccacc agtacagggt tattctttgt
     1921 ccgtcggcta agaatctgga taagccgttt cacttctctc gaacgaccaa caactgggtc
     1981 cagcttgttt tcttttgcta atttcgtcaa atcacgtgct aatgaatcta gcgtaggtgt
     2041 tccttgcggt gtttgccgtt tatcttgtac atttacattt ctacgacgat tcattccttt
     2101 tccagcagaa tcaccagcta gccccatttt ttgtttcaga acttgacggg cttttgaaag
     2161 gctgatacct aagttgatca tgattcgagt agccatgata ctttcatctt ttaataagct
     2221 catcaaaata tgttctgttc caactttttg agaaccagtt ttcttcactt cgctgctagc
     2281 taaagcaaaa acttgtttca ttcttggtga atatggaaga aagactcctt tagggtaaga
     2341 acggatacca ccataaccaa tcagatgttc aatttcttca taaatttccg tttcattcaa
     2401 atccatttca cgtaatactt ttccagcaat tccattttgc tcgaccacta aggctagtaa
     2461 aacatgttct gaactaactg attgatgttt caaccgtttt gcttcttctt gtgcaatttc
     2521 taatactgct tgggctcttt cagtaaaaag ttcttccatc taatctcact tcccatcttc
     2581 ataacttaaa cgttccagaa atgctcggag aatccgagca cgtgtatgat cttcatattc
     2641 attgaaattc aatgtgtttt ttgcaattgc actcaacatc aagtttcctt ctttttttgt
     2701 aattatttgg tcttcataaa gtttttgaat aatagcaaaa gcatttttct cactgattgt
     2761 atcatcaaat aactgattta tctgctcaag taattgtttt ttatctgaaa tccttacttt
     2821 tgcaattcgt atataaccac caccaccacg tttgctttct actagataac cacgttggat
     2881 ggtaaaacgg gtattaatca catagttgat ttgtgaagga acacagttaa agaggtctgc
     2941 catttcaatc cttcgaatct cgatcatttc actattttcc agaattttct ttatatatga
     3001 ttcaattaaa tccgaggtat tctgatgtgc catcttattc attccctttg actaatattg
     3061 accttaatta taacgaattt gataatagaa agaaagaaga acgctttttg ttaaataaat
     3121 cctttagaat caacaaaaaa aaatacggaa tatactattc cgtatcgacg cgcccaagag
     3181 gagtcgaacc cctaaccttt tgatccgtag tcaaacactc tatccagttg agctatgggc
     3241 gcatattcaa tttaaaa
//
LOCUS       DACEKG010000151         3209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEKG010000151 DACEKG010000000
VERSION     DACEKG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.160"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            116..976
                     /locus_tag="IW664_002717"
                     /pseudo
     CDS             116..976
                     /locus_tag="IW664_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="IW664_002718"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="IW664_002718"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ0850026.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="IW664_002719"
     CDS             1830..2678
                     /locus_tag="IW664_002719"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ0850027.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="IW664_002720"
     CDS             2688..3083
                     /locus_tag="IW664_002720"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ0850028.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT         1246 a          326 c          642 g          995 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttat
//
LOCUS       DACEKG010000152         3030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEKG010000152 DACEKG010000000
VERSION     DACEKG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.161"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            441..725
                     /locus_tag="IW664_002721"
     CDS             441..725
                     /locus_tag="IW664_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850029.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            728..1144
                     /locus_tag="IW664_002722"
     CDS             728..1144
                     /locus_tag="IW664_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850030.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1281..2000)
                     /locus_tag="IW664_002723"
     CDS             complement(1281..2000)
                     /locus_tag="IW664_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0850031.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2675..2770
                     /locus_tag="IW664_002724"
     CDS             2675..2770
                     /locus_tag="IW664_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0850032.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT         1017 a          567 c          482 g          964 t
ORIGIN      
        1 aaaaaaactc ttagaagcga ttttagagcc atttaaaccc acttaattat attctccatg
       61 aaattcgctc catgcgtcaa aagctgcgtc tgaaattccg ccgtcttcgt tcactagttg
      121 cattaatttt tccaacgatc gttttctttc gggtgtcata tttttatatt ccaacaattt
      181 ttcgtctgtt aaatttggtt atcgattaca cgtgcagcac tcccccccga attttaaaga
      241 gcataaggaa acgtgtaccc actttctaaa aaaagtgggt agataggctg tgcctatcac
      301 gtttctcaga aaaaaatttg atatcacttt ttcgccatcg tgggtagctt atgtatagca
      361 ttttggcaaa aaacagctat acaaatatag tatttgtatg gtatacataa attatacata
      421 attgcaaaag gagatttttt atggacattt tagttcggaa tattgatcca aagtttgttg
      481 caacaataga caaacgttgc caagaactca cgaataaaac aggagaaaaa tggagtcgaa
      541 ataaatatct aaaagtttta atcgaaagtg attttgaccg tcaacttacg acttacaaaa
      601 aagaccaatt cgatttattg ctcgataaat ttattgctat ccaaacagaa aatactcaaa
      661 tattaagcga gtatgtctac acaaataatc aaatcattga aatgcttatc ggaggaatga
      721 tataaccatg agaaaaactt ctgtccaaaa acatgttact ttgtacccta gtcatgttga
      781 cgttttagat tccatcatcg aaaaaaatcc agatatcaat aactactcgg aagccattcg
      841 ctttttatgt atgagttatg aacccgatac caacacgacc actaccagca atcaggcaaa
      901 actaaatgct atgggaaaag aaatttccat catgactgaa atcatcgcag aatttgctga
      961 tttatatcta aaagatacag gcgttcttat tggcacaaat tctaaagtat atcaagaagt
     1021 gaaagaaaaa atcgaagcta aactaaatcc atcaaatcga aatcgaccaa agaatcatca
     1081 aaaagaaacc agtcaaacag tcacttctag aaatttcgac ccaacaaatt ttctaaaacg
     1141 ataaaaaaca gataggagtt attcctatct gttttttatt tagaagcccc atacaatatg
     1201 ctagaatgta ctagcaatct tagtcggttg taagggactt gtctacttgc ttaatgtaag
     1261 gggggcgagt tcttttttat ttattcttct aaccagttac gcagagaaat ctttggcaaa
     1321 tctgacgtta ggcttgaaaa agtaaattct aatttggcga ttttattgcc cttttttgct
     1381 tttatttttg tgactttaag atcattgaag tattttttta gttctcgtaa tgcaggattt
     1441 aatacttttt gatcaatgtt acccatttga tagtattctg ggatatccat taagctccta
     1501 aactcttcaa tacgaactac ataataacca gtagaacggt actgcataag cagtcggaat
     1561 agagtttttg catacgtaga acgcaacgac gtgaaagcag ataattcaaa tctactaaat
     1621 tcagctgtta aaccattaat aatatattct aggtccggat taacactgac ttcaacaaac
     1681 ttttttgttt tattgatttc aaaaccagta aacaaaacaa agtagcgaat agaatcctca
     1741 tcttcagtac ggtaagtaag attaagcata tctttataga gcttttctaa atcaccgaca
     1801 aaggcttttg tagcagtcat cttataatca cttaactctt ttaattcctc gaaagaaaat
     1861 ctaacttttt tcaaaccacg atctttcata cgagcgcata atgcaaaaaa taaatccatc
     1921 tgcttagcat tgaaattttt cagcggtact aaattcaatt cattttgata tttgactgtt
     1981 aattccgttt tttcatccat tcctttcttc cccccttaca aataaataat aacctttaac
     2041 aaccaactcg taaataggtt ggttgttatt atacaaaagg atattagtac tcagataaaa
     2101 agttgttagt actcagataa aaagttgtta gtactcagat aaaaagttgt tagtactcag
     2161 ataaaaagtt gttagtactc agataaaaag ttgttagtac ccttctgtat ccgttgggag
     2221 agtaaggctc aactgccccc taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat
     2281 aaacgcgcgc gcgcgagcaa aatttcccgc caaaaaatca cccacttttt aaaataaaca
     2341 ggttcgcgca tgatattaaa ctgccaatct agctccctat ggtcgctgta agaaggagtg
     2401 atcgctacgc tctcacattt tacttgcagc agtcgccttt aggctccttc gccaaaaata
     2461 attcattagg gactcctttg tggcgtcctg agagctcgtc taagccgttc taagacaaga
     2521 ttcgataaaa actatgtcct tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta
     2581 acaaccccaa ggcaataaaa aacttaggct tatttttgct tttttattgt ataattgatt
     2641 gtcgcctatc cgacagtctt gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat
     2701 ttgctccctt cttgttgggc ttttaagcgc tttgttcagt tactggttga ataaccgaaa
     2761 caagaagtaa aacttaatta aggcgactca gcccacaaaa aaagccccag agaatccgac
     2821 ctctctgggg atttcgtgtg tttcgtgttc acttatttgc aatcaaatta taacagaaat
     2881 aaatatttat ttcaaaaata tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc
     2941 aacatttaca aataaagtcg ctctgagagc tcgtattttt cgatttaaga cgttttattt
     3001 agtttttggt gtatttcatt ggtccaagct
//
LOCUS       DACEKG010000153         3018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEKG010000153 DACEKG010000000
VERSION     DACEKG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.162"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1257
                     /locus_tag="IW664_002725"
     CDS             <1..1257
                     /locus_tag="IW664_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0850033.1"
                     /translation="VIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGT
                     VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEV
                     TDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKK
                     GTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDL
                     PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITE
                     GDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGN
                     TSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNI
                     FTISGGLILLGTLGLLGYKNRKKENE"
     gene            1391..2734
                     /locus_tag="IW664_002726"
     CDS             1391..2734
                     /locus_tag="IW664_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0850034.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2846..>3018
                     /locus_tag="IW664_002727"
     CDS             2846..>3018
                     /locus_tag="IW664_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0850035.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1199 a          407 c          571 g          841 t
ORIGIN      
        1 gtcattgaag taacgtatcc agacggtacg aaagatacgg tgaaagttcc agtagaagtg
       61 acagacaatc gttctgacgc cgataaatat gaaccaacag tagaaggcga aaaagtagaa
      121 attggaggca cagtagattt aacagataat gttactaact tgccagcgtt accagaagga
      181 acaacagtaa cagatgttac tcccggcggt acaattgata ccaacacacc aggcaattat
      241 gaaggtgtca ttgaagtaac gtatccagac ggtacgaaag atacggtgaa agttccagta
      301 gaagtgacag acaatcgttc taatgcagat aaatacaatc ctgaaggaca aaaagtgact
      361 acagacttaa ataaagagcc agatgcatct gagggaatta agaacaaaga agatttacca
      421 aaaggaacta cttatgcttg gaaagagaaa gtagatgtta gcacagctgg aaataaaaaa
      481 ggtactattg tagtgacata tccagatgga tcatctgatg aagttgaagt agacgttaca
      541 gtgacagata ctcgttccga tgcagataaa tataatcctg aaggacaaaa agtgactaca
      601 gacttaaata aagagccaga tgcatctgag ggaattaaga acaaagaaga tctaccaaaa
      661 ggaactactt atacttggaa agaaaaagta gatgttagta cagctggaaa caaaaaaggt
      721 actgtagtag taacatatcc agatggatct aaagaagaag tagaagttac tatttctgta
      781 gtagataaaa aagcaccgaa taaacctcaa gttgatccta ttacagaggg tgaccaaatt
      841 gttactggta aaactgaacc aaatgcagag gtgacagtta cattaccaga cggaagtcaa
      901 caccatggga cagctgataa aaatggtaat tttacagtta aagttcctaa attagaggca
      961 gggacaaaag tgatagtaac tgcaactgat gaatctggga atactagtga acctactaat
     1021 gtagttgttt cttccaacaa aaaagacagt ggaaaaaacg gtagtaaagg tagtaagact
     1081 gacaatcaag gtagcaacag caatcaagac aaaaatagag gcaaaagcca gtcctcaaaa
     1141 gtttttccta agacgggaga gagtgacagt aatatcttta ctattagcgg tggtttaata
     1201 ctgttaggaa cgttaggatt attagggtat aaaaatcgaa aaaaagagaa tgaatagaaa
     1261 atcaatttct taagtcttct ctgagaagag gaagaatctt tttcttcctc tttttgcttt
     1321 tttatgatgg gaaataagtg ttagttaaag atgaatatac tttttataga aaggaaaaga
     1381 atgtaattaa gtggaaaaaa tagtaattat tggttcaaac cattcagggc tagccgcagc
     1441 tcgatttcta aacttaagcg attcaaatta tgaagttatt ctaatcgata aaaaatcaga
     1501 aattagttac ttggaatgtg ggacatcgtt atttataaaa ggagaagtaa gtaatttaga
     1561 agatcttttt tatcaaaata gaatgacact aaattctgag ataaaaaagt tatttctaga
     1621 gacagaagtt gacaaaattg atattcaaaa taaaaagatt agttgtgtaa aaaaagatgg
     1681 tacaagattc tctcaagaat atgacaaagt tatattagct acaggttcaa agcaaattat
     1741 gctagatata ccggggaata atttagctaa tattttttcg ataaagaatt ttccaaatgc
     1801 aaaagagatt tttaaagctt tggatgatag tactattaaa aaagtaggaa ttattggagc
     1861 aggatatatt ggagttgaaa ttgctgaagc cataagaaaa agagggaaag aagtatatct
     1921 tttcgatgtt gcagatagag tgttgtcgac ttattacgac cgtagttttt cagataaagt
     1981 tgaagaaata ctatcaaaaa atggaataca tctttgtcta tcagaaagtc ctaaaaaata
     2041 tttaggaaga aaaaagattg aaaaaatagt cactacaagt ggtacccaat actctatgga
     2101 tatggtcgta caagctatag gatttctatc taatagtcca attggggaaa aggaactctt
     2161 gagagatgtt agtggagcat atctaactaa tgaataccag caaacaaata taaaagatat
     2221 atatgctatt ggggattgtg ctacaacttt ttttacgtcc attaatagaa gtagtgttga
     2281 tttttctatt tctaatgctc taagaacagc ttatattgca actcaacata ttaacaatca
     2341 agattttaca ccttctggtt cccaatttac aaatggcttt tctatattta attacaattt
     2401 ttacgcagca ggtttgaatt taaaaacagc acgattatta agtgagtcta tagattatat
     2461 agaaatagaa gatttcatta gacctgcttt tttgagaaaa aatagtcgtg taaaattaag
     2521 aattagcttt gaaaaaaata caaagagaat agtaggtgta caactttgta gtcaagaaga
     2581 tttatctgga ctattatcaa tgttttctct tgctatagaa gaacaaatta cattagacaa
     2641 attgaaaatg ttagattatt tatttcatcc tagttttagc caaccctata attttatttt
     2701 aaaggcagta acaggattaa acattgaaac ataatcgtta ctaaatgaaa tcaagaaata
     2761 tactgtatta ctcaattcat tgaaaatatt tatcataatt tttaatagaa aacactatta
     2821 aagagaaaaa agagaggtaa atcaaatgaa aaaatcttta aaacttgcat cagcagttat
     2881 attaatgtcc caactcctta tagcaacacc tttccatgtg ttggcaagcg aaaattcaac
     2941 atacaaaatt gcaaaagaaa gaaccgagac gactgaaatg agtaattccg atgtaacagc
     3001 agagagtagt gaactttt
//
LOCUS       DACEKG010000154         3011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEKG010000154 DACEKG010000000
VERSION     DACEKG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.163"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1053)
                     /locus_tag="IW664_002728"
     CDS             complement(106..1053)
                     /locus_tag="IW664_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0850036.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IW664_002729"
     CDS             1299..1763
                     /locus_tag="IW664_002729"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0850037.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IW664_002730"
     CDS             complement(1825..2868)
                     /locus_tag="IW664_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0850038.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          996 a          596 c          577 g          842 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta g
//
LOCUS       DACEKG010000155         2851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEKG010000155 DACEKG010000000
VERSION     DACEKG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.165"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            203..1171
                     /locus_tag="IW664_002731"
     CDS             203..1171
                     /locus_tag="IW664_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0850039.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1164..2195
                     /gene="vanA"
                     /locus_tag="IW664_002732"
     CDS             1164..2195
                     /gene="vanA"
                     /locus_tag="IW664_002732"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0850040.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2201..2809
                     /locus_tag="IW664_002733"
     CDS             2201..2809
                     /locus_tag="IW664_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0850041.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          841 a          551 c          692 g          767 t
ORIGIN      
        1 attttttagg aaaatctcaa ggttatcttt actttttctt aggaaattaa caatttaata
       61 ttaagaaacg gctcgttctt acacggtaga cttaataccg taagaacgag ccgttttcgt
      121 tcttcagaga aagatttgac aagattacca ttggcatccc cgttttattt ggtgcctttc
      181 acagaaaggg ttggtcttaa ttatgaataa catcggcatt actgtttatg gatgtgagca
      241 ggatgaggca gatgcattcc atgctctttc gcctcgcttt ggcgttatgg caacgataat
      301 taacgccaac gtgtcggaat ccaacgccaa atccgcgcct ttcaatcaat gtatcagtgt
      361 gggacataaa tcagagattt ccgcctctat tcttcttgcg ctgaagagag ccggtgtgaa
      421 atatatttct acccgaagca tcggctgcaa tcatatagat acaactgctg ctaagagaat
      481 gggcatcact gtcgacaatg tggcgtactc gccggatagc gttgccgatt atactatgat
      541 gctaattctt atggcagtac gcaacgtaaa atcgattgtg cgctctgtgg aaaaacatga
      601 tttcaggttg gacagcgacc gtggcaaggt actcagcgac atgacagttg gtgtggtggg
      661 aacgggccag ataggcaaag cggttattga gcggctgcga ggatttggat gtaaagtgtt
      721 ggcttatagt cgcagccgaa gtatagaggt aaactatgta ccgtttgatg agttgctgca
      781 aaatagcgat atcgttacgc ttcatgtgcc gctcaatacg gatacgcact atattatcag
      841 ccacgaacaa atacagagaa tgaagcaagg agcatttctt atcaatactg ggcgcggtcc
      901 acttgtagat acctatgagt tggttaaagc attagaaaac gggaaactgg gcggtgccgc
      961 attggatgta ttggaaggag aggaagagtt tttctactct gattgcaccc aaaaaccaat
     1021 tgataatcaa tttttactta aacttcaaag aatgcctaac gtgataatca caccgcatac
     1081 ggcctattat accgagcaag cgttgcgtga taccgttgaa aaaaccatta aaaactgttt
     1141 ggattttgaa aggagacagg agcatgaata gaataaaagt tgcaatactg tttgggggtt
     1201 gctcagagga gcatgacgta tcggtaaaat ctgcaataga gatagccgct aacattaata
     1261 aagaaaaata cgagccgtta tacattggaa ttacgaaatc tggtgtatgg aaaatgtgcg
     1321 aaaaaccttg cgcggaatgg gaaaacgaca attgctattc agctgtactc tcgccggata
     1381 aaaaaatgca cggattactt gttaaaaaga accatgaata tgaaatcaac catgttgatg
     1441 tagcattttc agctttgcat ggcaagtcag gtgaagatgg atccatacaa ggtctgtttg
     1501 aattgtccgg tatccctttt gtaggctgcg atattcaaag ctcagcaatt tgtatggaca
     1561 aatcgttgac atacatcgtt gcgaaaaatg ctgggatagc tactcccgcc ttttgggtta
     1621 ttaataaaga tgataggccg gtggcagcta cgtttaccta tcctgttttt gttaagccgg
     1681 cgcgttcagg ctcatccttc ggtgtgaaaa aagtcaatag cgcggacgaa ttggactacg
     1741 caattgaatc ggcaagacaa tatgacagca aaatcttaat tgagcaggct gtttcgggct
     1801 gtgaggtcgg ttgtgcggta ttgggaaaca gtgccgcgtt agctgttggc gaggtggacc
     1861 aaatcaggct gcagtacgga atctttcgta ttcatcagga agtcgagccg gaaaaaggct
     1921 ctgaaaacgc agttataacc gttcccgcag acctttcagc agaggagcga ggacggatac
     1981 aggaaacggc aaaaaaaata tataaagcgc tcggctgtag aggtctagcc cgtgtggata
     2041 tgtttttaca agataacggc cgcattgtac tgaacgaagt caatactctg cccggtttca
     2101 cgtcatacag tcgttatccc cgtatgatgg ccgctgcagg tattgcactt cccgaactga
     2161 ttgaccgctt gatcgtatta gcgttaaagg ggtgataagc atggaaatag gatttacttt
     2221 tttagatgaa atagtacacg gtgttcgttg ggacgctaaa tatgccactt gggataattt
     2281 caccggaaaa ccggttgacg gttatgaagt aaatcgcatt gtagggacat acgagttggc
     2341 tgaatcgctt ttgaaggcaa aagaactggc tgctacccaa gggtacggat tgcttctatg
     2401 ggacggttac cgtcctaatc gtgctgtaaa ctgttttatg caatgggctg cacagccgga
     2461 aaataacctg acaaaggaaa gttattatcc caatattgac cgaactgaga tgatttcaaa
     2521 aggatacgtg gcttcaaaat caagccatag ccgcggcagt gccattgatc ttacgcttta
     2581 tcgattagac acgggtgagc ttgtaccaat ggggagccga tttgatttta tggatgaacg
     2641 ctctcatcat gcggcaaatg gaatatcatg caatgaagcg caaaatcgca gacgtttgcg
     2701 ctccatcatg gaaaacagtg ggtttgaagc atatagcctc gaatggtggc actatgtatt
     2761 aagagacgaa ccatacccca atagctattt tgatttcccc gttaaataaa cttttaaccg
     2821 ttgcacggac aaactatata agctaactct t
//
LOCUS       DACEKG010000156         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEKG010000156 DACEKG010000000
VERSION     DACEKG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.166"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..197
                     /locus_tag="IW664_002734"
     CDS             <1..197
                     /locus_tag="IW664_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850042.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IW664_002735"
     CDS             314..811
                     /locus_tag="IW664_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0850043.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IW664_002736"
     CDS             898..1290
                     /locus_tag="IW664_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0850044.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IW664_002737"
     CDS             1274..>2778
                     /locus_tag="IW664_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0850045.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACEKG010000157         2756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEKG010000157 DACEKG010000000
VERSION     DACEKG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.167"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            49..1437
                     /locus_tag="IW664_002738"
     CDS             49..1437
                     /locus_tag="IW664_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ0850046.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1775..>2756
                     /locus_tag="IW664_002739"
     CDS             1775..>2756
                     /locus_tag="IW664_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAQ0850047.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAY"
BASE COUNT         1034 a          416 c          487 g          819 t
ORIGIN      
        1 ttcaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaagtaatc tcttgatttc tatactataa gataagaaaa ccactattta acaaggttat
     1561 tcgaacctta ttttaagtta cttattattt aatgtatcga aaaaggtgaa ataggtttgt
     1621 tataataaga attaagtaag acatgaattg tctaagcaaa tgtagcttat ccatttcatt
     1681 aaaatacata tggtgaaaaa acaagttcaa atagtaacta attttttaaa aaactgattt
     1741 ataaaaaata caacacgaat gaggaaaata accaatgaca gaacaaatcg aaaaaaattc
     1801 aaaaagctta tatcaggcac tgtggaattc cgcagatatc ctgcgttcaa aaatggacgc
     1861 taatgaatac aagagctatt tactaggctt ggtcttttat aaatacttat ccgacaacat
     1921 gctgcggtat gtctccgttc tattggagga agaaacggac gacctaaata aagcacaaga
     1981 cctttacgtt gaagcattta aagatgcaga tataaaggat gatctcttgt atgaactaaa
     2041 agatgagttt tcatatacca tcgcacccgc attgaccttt accgcgcaag tggcagccat
     2101 tcatgatgga tcttttcaat tagaagacct ggtacaaggt ttccgtgaca ttgaacaatc
     2161 cagcgaaaac tttgagaact tatttgaaga cattgatctg tattctaaaa aactggggtc
     2221 aaccccacaa aaacaaaata aaaccatcgc agatgttatg aaagaattag aagggttaaa
     2281 catggcgggg catgctggcg atgtcttagg cgatgcctat gagtacttaa ttggccaatt
     2341 cgcatctgaa tccggtaaaa aagccggtga attctatacc ccacaaccgg tcgcaaaatt
     2401 gatgacacaa attgttttac aaggaaaaga agataaaaaa ggcttttctg tttacgacgc
     2461 gaccatgggt tcgggctcgt tacttttgaa cgctaaaaaa tattcccatc aaccgggaac
     2521 catttcttat ttcggacaag agttgaatac gtctacctat aatttggcgc gtatgaacat
     2581 gattttgcat ggggttccca ttgccaacca acacttgcat aatgcggata ccttggatca
     2641 agactggcca acagaagagc caacgaactt cgatggggtg ctcatgaacc caccgtattc
     2701 tgcaaaatgg tcggctgata aaggcttctt agatgaccca cgcttctctg cctatg
//
LOCUS       DACEKG010000158         2719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEKG010000158 DACEKG010000000
VERSION     DACEKG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.168"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IW664_002740"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IW664_002740"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0850048.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DACEKG010000159         2650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEKG010000159 DACEKG010000000
VERSION     DACEKG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.169"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            36..197
                     /locus_tag="IW664_002741"
     CDS             36..197
                     /locus_tag="IW664_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850049.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IW664_002742"
     CDS             210..713
                     /locus_tag="IW664_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0850050.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IW664_002743"
     CDS             773..1162
                     /locus_tag="IW664_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0850051.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IW664_002744"
     CDS             1149..>2650
                     /locus_tag="IW664_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0850052.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACEKG010000160         2623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEKG010000160 DACEKG010000000
VERSION     DACEKG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.170"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IW664_002745"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IW664_002745"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0850053.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          941 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaa
//
LOCUS       DACEKG010000161         2483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEKG010000161 DACEKG010000000
VERSION     DACEKG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.171"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1014..1304)
                     /locus_tag="IW664_002746"
     CDS             complement(1014..1304)
                     /locus_tag="IW664_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850054.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IW664_002747"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IW664_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACEKG010000162         2468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEKG010000162 DACEKG010000000
VERSION     DACEKG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.172"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(493..1785)
                     /locus_tag="IW664_002748"
     CDS             complement(493..1785)
                     /locus_tag="IW664_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0850055.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IW664_002749"
     CDS             complement(2059..2319)
                     /locus_tag="IW664_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850056.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          823 a          459 c          372 g          814 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagt
//
LOCUS       DACEKG010000163         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEKG010000163 DACEKG010000000
VERSION     DACEKG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.173"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            206..2068
                     /locus_tag="IW664_002750"
     CDS             206..2068
                     /locus_tag="IW664_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0850057.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="IW664_002751"
     CDS             2068..>2399
                     /locus_tag="IW664_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0850058.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DACEKG010000164         2305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEKG010000164 DACEKG010000000
VERSION     DACEKG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.174"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..1125
                     /locus_tag="IW664_002752"
     CDS             79..1125
                     /locus_tag="IW664_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0850059.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IW664_002753"
     CDS             1474..2106
                     /locus_tag="IW664_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0850060.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          861 a          312 c          456 g          676 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctatt
//
LOCUS       DACEKG010000165         2230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEKG010000165 DACEKG010000000
VERSION     DACEKG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.175"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..390)
                     /locus_tag="IW664_002754"
     CDS             complement(94..390)
                     /locus_tag="IW664_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0850061.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="IW664_002755"
     CDS             746..1102
                     /locus_tag="IW664_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0850062.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="IW664_002756"
     CDS             1112..2011
                     /locus_tag="IW664_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0850063.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          728 a          380 c          424 g          698 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaagaaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ctggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac ttgaccgtta
     1741 ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag ataaatccat
     1801 cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc agctgacccg
     1861 agattttgtc ttgaaagaac agctgttaga tagctatgaa cccatgaaat tacgtgtcct
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat
//
LOCUS       DACEKG010000166         2189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEKG010000166 DACEKG010000000
VERSION     DACEKG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.176"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(325..528)
                     /locus_tag="IW664_002757"
     CDS             complement(325..528)
                     /locus_tag="IW664_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850064.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="IW664_002758"
     CDS             complement(987..1952)
                     /locus_tag="IW664_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0850065.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DACEKG010000167         2134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEKG010000167 DACEKG010000000
VERSION     DACEKG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.177"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            73..1032
                     /locus_tag="IW664_002759"
     CDS             73..1032
                     /locus_tag="IW664_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0850066.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(1155..2129)
                     /locus_tag="IW664_002760"
     CDS             complement(1155..2129)
                     /locus_tag="IW664_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0850067.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          749 a          364 c          327 g          694 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc tagaaaatca
      961 ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga tgatctgtct
     1021 aacttaattt gacaattaag aagttaaaaa cattacaaaa aagacaagga tatcacatca
     1081 agaaaaaatg ttttgaaaat atctttgcct ttttgtatta tatctatatt aaataatcaa
     1141 tcacaacaca ttaactaatt tatatattta atttgttgtt tttctataat tggataacta
     1201 attaacttat gactatctaa gtcttcttta ttcatatcaa ttgcagtaat ttgactgtct
     1261 tcatatgttc gataataata gattcctttg tcaacattgc aacaagaaga ataaattgta
     1321 tattcatatt ttccatcacc aacatcacac aaaccttttt gttgttctac tgaacctaag
     1381 atatggaaaa attgactaat actttctgac tctgaatctc cagatacaga attcagcttc
     1441 gtaaaagtcg ctttaacaaa acgagatact gaggataaat ctccaggcaa gcctatccct
     1501 cccataccgc ggctataggc attcaaactt atttgatttg aaaaattatt tttaggagtt
     1561 tcactcgata agacacgata attgtttaaa ttaaataatt gatagtcaaa tgaaggatta
     1621 ttggtaagaa cgcccacagg gttatcatat atatgaagtc catctttcat actttcaatg
     1681 acaattgatt tttctttatc agctaatagc caatgtaaag gggataaagg aagttcatca
     1741 ctataattta tatttgctaa attgatattt tttaacaatt ttttagtttc tcctactgtt
     1801 gagcattgtc ctaaaatcca aggaataaat tcaaaaggag atacattgtc tttcccttct
     1861 tgtatttctt tataatcagc atacccagaa aaatttagcc cagccatact caatcctttt
     1921 tcatttgtcg catcgtaata aagagggtag tcagctatac cagcggcaat accaatcatt
     1981 gcataatgag tatccaaatc atttaccttt cgaaaattca acttataatt tcttggagta
     2041 acagtaacta cttcattgta agatatttca taatcaaaat tccttccaaa ataatgatca
     2101 cttgttacat aagtaataga cgtacacata gttt
//
LOCUS       DACEKG010000168         1917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEKG010000168 DACEKG010000000
VERSION     DACEKG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.178"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..98)
                     /locus_tag="IW664_002761"
     CDS             complement(<1..98)
                     /locus_tag="IW664_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAQ0850068.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKV"
     gene            complement(113..1360)
                     /locus_tag="IW664_002762"
     CDS             complement(113..1360)
                     /locus_tag="IW664_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0850069.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1436..1582)
                     /locus_tag="IW664_002763"
     CDS             complement(1436..1582)
                     /locus_tag="IW664_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0850070.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1686..>1917)
                     /locus_tag="IW664_002764"
     CDS             complement(1686..>1917)
                     /locus_tag="IW664_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0850071.1"
                     /translation="LEVLQKVYLKLEKQKYELDPGPLINRLVNYLYFTAYTNKIRFTE
                     YQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
BASE COUNT          687 a          306 c          208 g          716 t
ORIGIN      
        1 aatactttat cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt
       61 ataatttgat cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca
      121 acaaaataac ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg
      181 gattcttttt attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg
      241 ttgttattcc ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg
      301 atatcgaaat ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt
      361 gactctctat agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt
      421 caaatattgg aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct
      481 tattgttatt tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta
      541 tattttttaa ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta
      601 attcttttat ttctttttcc aatatcgaat catggttttg attagcgatt tcttttaaac
      661 tcagcaataa atttttatag tcatgtctaa atctggcaaa tttttcttga tctttttcca
      721 gtgaatcagt atattttttt agataaacca attcttttct tttcaattgc tgttcatatt
      781 gttctcgttg cctaatagtt attctaataa ataaaaatag aacaacaaac atttgaatga
      841 ttaaaaatat taagacacca aacacaaaat gatcaaatac ttgatattga tgagcaacat
      901 aagaaacaaa taaaccaatt gtaagtaaat aaatcatgaa tatagcagta agtttagaac
      961 tggactgttc aacaatccta tttatagcta attttctata taaccaaata aacatcagca
     1021 aaaacaaaga atcaagcatt atctggaata ctacataacc ataccctttt atcccttcaa
     1081 atgaaaacat atacgtcata accatggaag taataataga tctgaaatca tcaataagca
     1141 tacataatag taatttattt aaaagcacta tattagtttt cttcttttcc tttaatataa
     1201 aataacttcc gattataggt atagctgcgg aaacatcagt aaataattca acaaaaaaag
     1261 tgagtggtat caataaacat acaaaaagat attttctttt gttaagatca gtattagtca
     1321 tataaaataa gagaaaaaag aaaaagtttc ccacgaacat tcttaataga taagcttttg
     1381 tatcgatagt aatcaagaga tcccctcctg attttaggat atcataaaaa aaattttagt
     1441 tacacttttt aaataatgaa aaaacacatt ccactaaatt tgatgccggt tttccttgag
     1501 gttttgttcc agcacctcct ttaatttttt taagctcttg atctgaacat tgtttagcat
     1561 ttagtctatt tttttcttcc attacgattc cccctacaaa ctataaatac ttttatgtca
     1621 ctgattcaat atacctattt ttctaatcaa aaaattttga gacatattta atattataag
     1681 tatcattaaa attgagattt atctccataa tctgctcgat ataaaccatt tattccagca
     1741 gttcttccaa tttcactcaa atttcttatt aattcctctt gatattcagt gaatcttatt
     1801 ttattagtat aagcagtaaa atatagataa ttcaccaatc tatttattaa aggaccggga
     1861 tctaattcat atttttgttt ttctaatttt aaatatacct tttgtaaaac ctcaaga
//
LOCUS       DACEKG010000169         1839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEKG010000169 DACEKG010000000
VERSION     DACEKG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.179"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(298..1485)
                     /locus_tag="IW664_002765"
     CDS             complement(298..1485)
                     /locus_tag="IW664_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0850072.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          566 a          372 c          321 g          580 t
ORIGIN      
        1 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
       61 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
      121 atgagacagt caagtccaca atcttgtgta aaagttcaac ctcatcaaaa gcacaatgtt
      181 ttgcgttgtg aacctaactg ccatgccttt ctttctgagg agagaggtca ggagcagtag
      241 cgcagcgact cttgatgtct ctcctcagaa agaagagact cgcacaggtt catggtgcta
      301 aatgttattc gaattaaatt ctaaatattt tcgtggagaa gccagccact cagtgtcttt
      361 ttccatcaaa agtgttccaa ttatccgagt gatggaattg atatttggaa aaatccgaat
      421 cactcgttct ctccggcgaa tctcttcgtt caaccgttcc aacacattgg ttgtacgaat
      481 ccttcggcgg atattttcag gaaaagacaa aatttgaata gcatcttcaa agccgttttc
      541 taaaatgtcg caagcagcac ttaattttga atcacaacca tatttttcca agaagtgctc
      601 ttttctttca cgtgtcaatt caagttcaga ggctttaaag atactcttca actcgtcttt
      661 tacatcagaa gaaactttct ttggcagctt atcaaaaata tttctcaaaa agtgggcttg
      721 gcatctttgc cagctcgcac ctaaaaagtt cttgcgaaca gccttaacta accctttgtg
      781 tgcatcagaa atgaccagtt ttacaccgaa caaacctcgt tgaacgaacg attgaaaaac
      841 ggtatcccaa ttatcttccg attcgccgtc ttgaacatca aaaccaagaa tcgtacgtcg
      901 tccatcagaa tcaatgccga taccaatata tacacctttt gagacaatac gatgattttc
      961 ccttactttc atatacaggg catcacacat gaaaaatgga tattccgagc cttctaggct
     1021 ccggtttctc cactcaaaaa caaatggatc caactgtttc atcagatttg aaacgaatga
     1081 tttagaaaca gtcgcaccat ccgttagcag ttcaactgtt ttagttactt tgcgagtgga
     1141 gacaccagaa atgaccattt caatcatggc agcgatcaaa gctttttcgc ttcgctgata
     1201 tctttcaaag atagtaggac taaacttacc gtctctggtt cgtggaacat gaagttctaa
     1261 cgtaccaatt cgagttgtaa acgaacgatc gtagtaacca tttctttgtg attgccgttc
     1321 ttctgaacgt tcataggcat ttgcctgaat atattgattt cgttgttctt ccatcagctg
     1381 attaaacatg atagtcataa gtgtttttgc ggtatcattc gcaccgcttt cgtttataat
     1441 tgcttgaatt tcttcgcttt caaaagtaaa atgtacttga gtcattgata tcagtcctat
     1501 tcgagtatgt tttctgcaaa aaacattgta ctcctaactg agatcaatgg cttttttgat
     1561 gcttcacttt tacacaatta tatggacgta atcaatgaga cacaattatg gtttaaattt
     1621 gacccttcga atagatttat caaagatttt tataaagtat ggaattcaga agttttcttt
     1681 ttagcaatcg aagatagctt attaatcaat ctctactatt ctaataagaa ctatttcaaa
     1741 attcctgctg cgaaaactag aatgaagaag gatgtatact ttttgtttga tatcgtgact
     1801 gacgtgccgg acgcacgtag cgatcatcgg cgttatgac
//
LOCUS       DACEKG010000170         1701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEKG010000170 DACEKG010000000
VERSION     DACEKG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.180"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..255
                     /locus_tag="IW664_002766"
     CDS             <1..255
                     /locus_tag="IW664_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850073.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="IW664_002767"
                     /pseudo
     CDS             258..411
                     /locus_tag="IW664_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="IW664_002768"
     CDS             434..1639
                     /locus_tag="IW664_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0850074.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DACEKG010000171         1581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEKG010000171 DACEKG010000000
VERSION     DACEKG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.181"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1297
                     /locus_tag="IW664_002769"
     CDS             128..1297
                     /locus_tag="IW664_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0850075.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          568 a          254 c          258 g          501 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
     1021 gcagtatcca agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt
     1081 ccctcacgcc atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca
     1141 gctaaaacga tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt
     1201 cttcatttct caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt
     1261 taaagaaatt caggatacat tagagtcgat gatttaactg taattaacag aatggatttt
     1321 ccatttacat ataattcttg acgctaccat aatctaaata accatcatat tcatctacta
     1381 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa
     1441 tctctgcatc cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt
     1501 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt
     1561 tacttccatg gataataata a
//
LOCUS       DACEKG010000172         1515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEKG010000172 DACEKG010000000
VERSION     DACEKG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.182"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            287..694
                     /locus_tag="IW664_002770"
     CDS             287..694
                     /locus_tag="IW664_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850076.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IW664_002771"
     CDS             708..1109
                     /locus_tag="IW664_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850077.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IW664_002772"
     CDS             1111..1482
                     /locus_tag="IW664_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850078.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DACEKG010000173         1512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEKG010000173 DACEKG010000000
VERSION     DACEKG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.183"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(155..1174)
                     /locus_tag="IW664_002773"
     CDS             complement(155..1174)
                     /locus_tag="IW664_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0850079.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1185..1391)
                     /locus_tag="IW664_002774"
     CDS             complement(1185..1391)
                     /locus_tag="IW664_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0850080.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          419 a          313 c          260 g          520 t
ORIGIN      
        1 cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac tattatgggt
       61 gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata gttgtcaata
      121 tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc
      181 aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg
      241 cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa gatacatgat
      301 agccaacatt tccagaagta ttataagctt gatttacatc tgatctataa attccatcag
      361 ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata tactcgtatt
      421 tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt tctccaaact
      481 gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca ccagctcgtg
      541 ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta aatccatgag
      601 ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa ccgtaaggat
      661 ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt cccggttggt
      721 tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct ctagccaatt
      781 caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt tgtaactcaa
      841 tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca taaccaggtt
      901 gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa gcgctagtcc
      961 atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta gccgtttcat
     1021 gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg cccaaattaa
     1081 attcattgtt gatagtatag gcaaatccgt taattggcaa taaaaaaaga gccgttaaca
     1141 ggctcatcgc agtaatagta attttctttt tcatatctat cctcctattt atctgatgaa
     1201 attttcttat acgttcgatt tgatacacct aaaatactcc ctagaaatgc tccaaaacct
     1261 gtaataatca tcaccgttat atctgtgtac cgccagttaa caccttttcc gacaagaccg
     1321 acaaaagtcg ctaaagcggg gataactact atagcgatcc atttcaaaat atcaaaggtt
     1381 ctattgttca tactatccac ctcccttcat tggttgggct actttctttt tcaatcgctt
     1441 gttttcttct atcaatattt ctctttctga aagcaaggca tcaatttgcc cgtccagttg
     1501 ttcaatttta tc
//
LOCUS       DACEKG010000174         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEKG010000174 DACEKG010000000
VERSION     DACEKG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.185"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            108..1040
                     /locus_tag="IW664_002775"
     CDS             108..1040
                     /locus_tag="IW664_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0850081.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IW664_002776"
     CDS             1112..>1456
                     /locus_tag="IW664_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850082.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACEKG010000175         1409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEKG010000175 DACEKG010000000
VERSION     DACEKG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.186"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1359
                     /locus_tag="IW664_002777"
     CDS             <1..1359
                     /locus_tag="IW664_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0850083.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DACEKG010000176         1353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEKG010000176 DACEKG010000000
VERSION     DACEKG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.187"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..417)
                     /locus_tag="IW664_002778"
     CDS             complement(<1..417)
                     /locus_tag="IW664_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0850084.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDL"
     gene            complement(414..1067)
                     /locus_tag="IW664_002779"
     CDS             complement(414..1067)
                     /locus_tag="IW664_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0850085.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          424 a          235 c          126 g          568 t
ORIGIN      
        1 taaatctttc gcatcttttt gcatatcagc tgtagaaaca aattgtagat ttgttttttt
       61 accatcatta agctttattt ctctttcttc aattatcaaa gaattactat cactttctaa
      121 attaacattt atattatctt tgatttgtat ttcattctta ttattttttt cttttatgaa
      181 tacttttatt tcactactct tggaataaaa ctccaaagtt tgttctatat attttatttc
      241 ttttccattc atattactag aaatttcatt tgccatatta tgaatctttt cttttctcgt
      301 ctccaaatat gttttaggaa agataaaaaa aattaatgaa tgaactaata taattattat
      361 tccaagaaca gaaaatattt gtatgaacat ttttggaaat atctttaatt ttttcatttt
      421 ctctccaatt tatatcctac atttcttata gtagttatac agtctaattg taattttttt
      481 ctaagttctt ttatatatac atctattact cgatcataag gagtttcttc gctatctttc
      541 catacataat caataatttg cattcttgtt aacacttgtc cattattatc caataaacat
      601 tttaatactt ctagttcttt tgcatttaca tctatttctt caccatttat tttagctgta
      661 tagctattaa aattaaccga tagatcctta tattcaaact tctctaaatg tccataattc
      721 ttcttaatta aagaatctat tctagctttt aagaccggca atgaaaatgg cttttctaca
      781 tatccgtcta ctaaattagt aaatgcatca attttatatt cttcatcact aaatgcagtc
      841 aagattagaa ttggtaaatt gcttttcttt ctaatttctt tcaacacttc taaaccattt
      901 ataaaaggta tctgaatgtc taagataacc aaatttatat cgctattaaa ttttgacaag
      961 gcttctcttc catctttagc ttgaataaca gtatatccaa attctgaaag atattcactt
     1021 attccctctc ttatcatatt atcatcttca acagttaata ttttcatata tacctccact
     1081 taagcctata cactagatat tatacagcat attatggctc tataatatct agtgttggtg
     1141 acgccttctg ttgccaatac tttttctttt ttttaataaa tgcaaacaga caccttgaaa
     1201 tcctttagaa aaaaggcttc tataatattt tgtgttgttg gaaaatcgaa ggcgattttc
     1261 tactaacact ttttcttcgt ttcaactcaa aaagagactc acttttggta aaatttagtc
     1321 gaccaaaaca tcaccctacc aaaggagtcg ctc
//
LOCUS       DACEKG010000177         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEKG010000177 DACEKG010000000
VERSION     DACEKG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.188"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>1352
                     /locus_tag="IW664_002780"
     CDS             82..>1352
                     /locus_tag="IW664_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0850086.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFL"
BASE COUNT          459 a          260 c          257 g          376 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg at
//
LOCUS       DACEKG010000178         1319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEKG010000178 DACEKG010000000
VERSION     DACEKG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.189"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="IW664_002781"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="IW664_002781"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0850087.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..>1319
                     /locus_tag="IW664_002782"
     CDS             1131..>1319
                     /locus_tag="IW664_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850088.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTE"
BASE COUNT          393 a          269 c          202 g          455 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agagcaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaa
//
LOCUS       DACEKG010000179         1316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEKG010000179 DACEKG010000000
VERSION     DACEKG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.190"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..>1316
                     /locus_tag="IW664_002783"
     CDS             99..>1316
                     /locus_tag="IW664_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0850089.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          430 a          271 c          240 g          375 t
ORIGIN      
        1 gacaccagca tcttttttat tttccaaaac atatagaaaa gacaccttga aatcctttag
       61 aattaatttg gcgaaccaac ccaaaaggag gaatcaagat gtcttacact caccttatca
      121 aagaaacatt agatattcta gacttatcag ttacttttaa cgaaaattgt ctcacaaaag
      181 aaaaatacaa agggcaaatc tgtcatattt atcgtggaaa cttgatttat acagcccaag
      241 aatgcatcca ctgtaaacac cagattgctt cagatattgt gcgttggggt acaacaaccg
      301 tccggttgtt aatgaacgat gtttctgaat accgtaccta cctcgaattg aaaaagcaac
      361 gcttcaaatg caaagcttgt cagcggacct ttgtcgcaga tacttctgtt gcgaaaaaac
      421 attgtttcat cagtgagaaa gtccgctggt ctgtcgtgac tcgtctgaaa aaaaatacct
      481 caatgacaga gatcgccgct cagaaaaatc tatcggtctc ttctgtttac cgtattatga
      541 agcgatttta tcgtccgttg aatccttttc ggacaccgtt acctaaggta ctttgttttg
      601 atgaattcaa atcagttcgg ggtgtttcag gtgctatgag tttcatcatg atggatggac
      661 aaacacagcg cttattggac attgtcgaga accgacaact gcctttttta aaacgttatt
      721 ttagtcattt ttcacgagaa attcgtgaag cggtcgaatg gatcgtgatt gatatgtacg
      781 ctccttatgt atcgctcgtt aaaaagctgt ttccaaaagc acagctgatt attgaccggt
      841 ttcacatcgt ccaacatatt ggtcgtacct ttcgcaatca tcgaatcaaa gaaacgaacc
      901 aactattaaa aagcaaagaa caaaaacact atcaattagg taagcaatta aaaagatact
      961 ggaaacttct tcaaaaagat gaacgcaagc ttgattatac tcgacgcctt tggcgacctg
     1021 gcttcaaagc tcatttaaca gagaccgata ttgtggatcg cttactgaaa gggagccctg
     1081 ctttgcgtgt aggctatcag ttatatcaag actttcttta cgctgtaaag gaacgagact
     1141 acgtatcgtt tgaagaacta cttaccaata atatcatgct tcccgaaggc tatcagacga
     1201 ccttgagaac gttccagaaa tttctgccac aaatcaaaaa tgctttacaa caaagttatt
     1261 caaatggccc acttgaatgt ctgaacaacc atatcaaagt gttaaaacga aatgca
//
LOCUS       DACEKG010000180         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEKG010000180 DACEKG010000000
VERSION     DACEKG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.191"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..1248
                     /locus_tag="IW664_002784"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IW664_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACEKG010000181         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEKG010000181 DACEKG010000000
VERSION     DACEKG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.192"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..716
                     /locus_tag="IW664_002785"
     CDS             24..716
                     /locus_tag="IW664_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0850090.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
     gene            915..>1311
                     /locus_tag="IW664_002786"
     CDS             915..>1311
                     /locus_tag="IW664_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0850091.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEI"
BASE COUNT          447 a          247 c          256 g          361 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt ttggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      841 ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttggc taccaattca
      901 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
      961 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
     1021 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
     1081 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
     1141 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
     1201 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
     1261 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc g
//
LOCUS       DACEKG010000182         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEKG010000182 DACEKG010000000
VERSION     DACEKG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.193"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..532
                     /locus_tag="IW664_002787"
     CDS             35..532
                     /locus_tag="IW664_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0850092.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="IW664_002788"
     CDS             complement(569..1252)
                     /locus_tag="IW664_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850093.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACEKG010000183         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEKG010000183 DACEKG010000000
VERSION     DACEKG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.194"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..947
                     /locus_tag="IW664_002789"
     CDS             414..947
                     /locus_tag="IW664_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0850094.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACEKG010000184         1188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEKG010000184 DACEKG010000000
VERSION     DACEKG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.195"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            141..398
                     /locus_tag="IW664_002790"
     CDS             141..398
                     /locus_tag="IW664_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850095.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IW664_002791"
     CDS             complement(630..1043)
                     /locus_tag="IW664_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0850096.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DACEKG010000185         1134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEKG010000185 DACEKG010000000
VERSION     DACEKG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.196"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..>1134
                     /locus_tag="IW664_002792"
     CDS             join(55..331,331..>1134)
                     /locus_tag="IW664_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0850097.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          425 a          192 c          217 g          300 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acac
//
LOCUS       DACEKG010000186         1129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEKG010000186 DACEKG010000000
VERSION     DACEKG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.197"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(87..956)
                     /locus_tag="IW664_002793"
     CDS             complement(87..956)
                     /locus_tag="IW664_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0850098.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
BASE COUNT          319 a          230 c          171 g          409 t
ORIGIN      
        1 attttctctt tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca
       61 tgcgacaatc gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt
      121 tcttttggct cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact
      181 gtgtgctttt tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct
      241 tccatgcttt cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta
      301 ggcaaatcaa ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc
      361 aatacatgga tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat
      421 tggcgttctt ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt
      481 gatagtttac tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct
      541 ggaatttctc tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct
      601 gggtctacga gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa
      661 ttcattttac ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat
      721 gaaaaacggt tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc
      781 agcagcaaca tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct
      841 cttttcaaat caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc
      901 acccgaatgg ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt
      961 cttgctcccc tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata
     1021 actttgattt tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt
     1081 aatgagtaaa aactcttata aaaagaggtt gtgaaaaagc tgttctgca
//
LOCUS       DACEKG010000187         1069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEKG010000187 DACEKG010000000
VERSION     DACEKG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.198"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1069
                     /locus_tag="IW664_002794"
     rRNA            <1..>1069
                     /locus_tag="IW664_002794"
                     /product="23S ribosomal RNA"
BASE COUNT          288 a          238 c          323 g          220 t
ORIGIN      
        1 atgtctgtcc aagcagtaag tctgaagagg agtcaaatgc ttcttttctt aaggacaagc
       61 tgtgatgggg agggaaataa tagtaccgaa gttcctgatg tcacactgcc gagaaaagct
      121 tctagtgaga aaacagcggc ccgtaccgca aaccgacaca ggtagtcgag gagagaatcc
      181 taaggtgagc gagagaactc tcgttaagga actcggcaaa atgaccccgt aacttcggga
      241 gaaggggtgc tgatcatacg atcagccgca gtgaataggc ccaagcgact gtttatcaaa
      301 aacacaggtc tctgcaaaat cgtaagatga agtatagggg ctgacgcctg cccggtgctg
      361 gaaggttaag aggagtgctt agcgcaagcg aaggtacgaa ttgaagcccc agtaaacggc
      421 ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacccgc
      481 acgaaaggcg taacgatttg ggcactgtct caacgagaga ctcggtgaaa ttttagtacc
      541 tgtgaagatg caggttaccc gcgacaggac ggaaagaccc catggagctt tactgtagtt
      601 tgatattgag tgtctgtacc gcatgtacag gataggtagg agccgtagaa atcggaacgc
      661 tagtttcgat ggaggcgctg gtgggatact acccctgcgt tatggccact ctaacccgca
      721 ccactaatcg tggtgggaga cagtgtcaga tgggcagttt gactggggcg gtcgcctcct
      781 aaaaggtaac ggaggcgccc aaaggttccc tcagaatggt tggaaatcat tcgaagagtg
      841 taaaggcaga agggagcttg actgcgagac caacaagtcg agcagggacg aaagtcgggc
      901 ttagtgatcc ggtggttccg catggaaggg ccatcgctca acggataaaa gctaccctgg
      961 ggataacagg cttatctccc ccaagagtcc acatcgacgg ggaggtttgg cacctcgatg
     1021 tcggctcgtc gcatcctggg gctgtagtcg gtcccaaggg ttgggctgt
//
LOCUS       DACEKG010000188          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEKG010000188 DACEKG010000000
VERSION     DACEKG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.199"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>994)
                     /locus_tag="IW664_002795"
     rRNA            complement(<1..>994)
                     /locus_tag="IW664_002795"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACEKG010000189          922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEKG010000189 DACEKG010000000
VERSION     DACEKG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.200"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..625
                     /locus_tag="IW664_002796"
     CDS             <1..625
                     /locus_tag="IW664_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0850099.1"
                     /translation="RDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGY
                     LSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFG
                     EIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGY
                     HVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            815..888
                     /locus_tag="IW664_002797"
     tRNA            815..888
                     /locus_tag="IW664_002797"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:849..851,aa:Ile,seq:cat)
BASE COUNT          302 a          170 c          187 g          263 t
ORIGIN      
        1 aagagatagt gcaaaaaaat atggaattcc attgactctt gatcaaggaa cttctttatt
       61 tacaaaagga atcatttctc atttgtgggt gacaaattat gtttggggga accacacaga
      121 tccatatggt tacttatcgc aaatgggagt tagcaaagaa aagcttgctt atgatttagc
      181 tcatggattt accgatgaaa atccaacaac ttctgaaaac aagcctgtca ttgatccaac
      241 acgagctggt gcagctaatc ctacgctgac agatggaaca aatcacgccc acattgatca
      301 gtttggggaa atcgaaaacg caaacttgca tgtggctgga tggcacattg ctaactataa
      361 atacgagtat attttcatta tggactacaa tactgggaaa gaattagctc gagtaagagc
      421 tgatggaatt tatagaccag atgtaaacca agcttataat acttctggaa atgttggtta
      481 tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa gtctatgtaa tgatgcgggc
      541 aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg acaaacgttg
      601 gtatttaaat attcctaaac gataaaaata gctcctcgtt gaggagcagt acataactat
      661 attgacaact ataaaaatta ttcgataaaa tagtgatgtt atcgcatatc ttcactatca
      721 cccataaata gtcacactcc aagctatgcg ataacaggtt tgttgccaca cattctactg
      781 gttgattgtt tatggcttta tgtggcaaca accagtaccc ttagctcagt tggtcagagc
      841 agacggctca taaccgtccg gtcgtaggtt cgagtcctac agggtacatt aacgtagcca
      901 tttgaatcgt tctgtgttag aa
//
LOCUS       DACEKG010000190          912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEKG010000190 DACEKG010000000
VERSION     DACEKG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.201"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            68..183
                     /gene="rrf"
                     /locus_tag="IW664_002798"
     rRNA            68..183
                     /gene="rrf"
                     /locus_tag="IW664_002798"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            193..266
                     /locus_tag="IW664_002799"
     tRNA            193..266
                     /locus_tag="IW664_002799"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Asn,seq:gtt)
     gene            306..395
                     /locus_tag="IW664_002800"
     tRNA            306..395
                     /locus_tag="IW664_002800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:340..342,aa:Ser,seq:gga)
     gene            410..481
                     /locus_tag="IW664_002801"
     tRNA            410..481
                     /locus_tag="IW664_002801"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:443..445,aa:Glu,seq:ttc)
     gene            497..569
                     /locus_tag="IW664_002802"
     tRNA            497..569
                     /locus_tag="IW664_002802"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:530..532,aa:Val,seq:tac)
     gene            579..651
                     /locus_tag="IW664_002803"
     tRNA            579..651
                     /locus_tag="IW664_002803"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            687..762
                     /locus_tag="IW664_002804"
     tRNA            687..762
                     /locus_tag="IW664_002804"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:720..722,aa:Phe,seq:gaa)
     gene            764..844
                     /locus_tag="IW664_002805"
     tRNA            764..844
                     /locus_tag="IW664_002805"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:798..800,aa:Tyr,seq:gta)
BASE COUNT          222 a          199 c          244 g          247 t
ORIGIN      
        1 aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat aaaagataac
       61 accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga acacagaagt
      121 taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag gacgtcgcca
      181 cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta atcatgatgt
      241 cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg agcttctttc
      301 actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt agatggtgcc
      361 aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg gcccgttggt
      421 caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc cgtacgggtc
      481 attttaattg aatgttggag gtttagctca gctgggagag catctgcctt acaagcagag
      541 ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gtcgttcttg
      661 ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag caacggattg
      721 aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag tagcgaagtg
      781 gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa tccactctcc
      841 tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc gttagtgtgg
      901 gttcaattcc tg
//
LOCUS       DACEKG010000191          897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEKG010000191 DACEKG010000000
VERSION     DACEKG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.202"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..853
                     /locus_tag="IW664_002806"
     CDS             <1..853
                     /locus_tag="IW664_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0850100.1"
                     /translation="IISLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGEL
                     KQQINDLYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMK
                     YNIGKYKERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKK
                     YEILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWL
                     YIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFI
                     FKIKIFS"
BASE COUNT          404 a           61 c          122 g          310 t
ORIGIN      
        1 tattatttct ttaatttatg caaaatcaat aaaaaataat atacaagaaa taaaaattgt
       61 tactgataaa atgatgaaac ttgataaaaa aatgagttta aaagttagct ctaatgatga
      121 agttggagaa ttaaaacaac aaattaatga tttgtattct actttattaa gaacaattga
      181 cgatttggaa tttaaaaata aagaaatttt aaaattagaa aagttaaaat atgacttttt
      241 taaaggtgca tcccatgaac ttaaaacccc tcttgctagt cttaaaataa tactggaaaa
      301 catgaaatac aatattggaa aatataaaga aagagatatt tatattaatg aatgtattga
      361 aatcgttgat agtttaacaa aaaatatttc tcaaatatta tcagttcatt ctatagaaaa
      421 tttgaataat gatgaagaat atttgaaaat aaatgataca ttagaagaca tattaaaaaa
      481 gtatgaaata ttagcaaatc aaaagaaaat aactgtcaat aattatatat tagacgagaa
      541 tgtttatata ggaaaaacag ctttaaagat tattctatct aatttgatta gtaatgcggt
      601 aaaatatact gatgtaaatg gggtaattaa tattgggata gttaatgatt ggttatatat
      661 agagaattca tacggtaaca ataaaatttc gaatatggat aaaatatttg atgttaaatt
      721 tgatttaaat aaggaaaata gtaatggatt aggtttatat attgtaagta atattctgaa
      781 taattacaat atagaatata aagtattgca ggatgaagaa ttttttatct ttaaaataaa
      841 aatattttct taattaagat ataattttgc aatagttatt gaaaaataga gacttat
//
LOCUS       DACEKG010000192          860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEKG010000192 DACEKG010000000
VERSION     DACEKG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.203"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..856
                     /locus_tag="IW664_002807"
     CDS             <1..856
                     /locus_tag="IW664_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0850101.1"
                     /translation="KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRA
                     SFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPET
                     YTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIR
                     HGKTFLTK"
BASE COUNT          273 a          173 c          165 g          249 t
ORIGIN      
        1 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
       61 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      121 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      181 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      241 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      301 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      361 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      421 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      481 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      541 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      601 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      661 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      721 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      781 gtacggcttt cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac
      841 atttttaacc aaataaacag
//
LOCUS       DACEKG010000193          846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEKG010000193 DACEKG010000000
VERSION     DACEKG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.204"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            312..608
                     /locus_tag="IW664_002808"
     CDS             312..608
                     /locus_tag="IW664_002808"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0850102.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..846)
                     /locus_tag="IW664_002809"
                     /pseudo
     CDS             complement(531..846)
                     /locus_tag="IW664_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          273 a          167 c          129 g          277 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtattaa gcagatataa
      721 tgccactttt tccccacgcg aacataagta agatccgtaa cgatggcttc caatggctgt
      781 tcttgtgtaa aggtccgatc taatacattc gctgttttcg cttcattaca agctgttcgt
      841 tgccct
//
LOCUS       DACEKG010000194          845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEKG010000194 DACEKG010000000
VERSION     DACEKG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.205"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(21..94)
                     /locus_tag="IW664_002810"
     tRNA            complement(21..94)
                     /locus_tag="IW664_002810"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IW664_002811"
     tRNA            complement(108..181)
                     /locus_tag="IW664_002811"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IW664_002812"
     tRNA            complement(209..281)
                     /locus_tag="IW664_002812"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IW664_002813"
     tRNA            complement(301..374)
                     /locus_tag="IW664_002813"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="IW664_002814"
     tRNA            complement(384..457)
                     /locus_tag="IW664_002814"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="IW664_002815"
     tRNA            complement(468..553)
                     /locus_tag="IW664_002815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="IW664_002816"
     tRNA            complement(570..641)
                     /locus_tag="IW664_002816"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="IW664_002817"
     tRNA            complement(653..725)
                     /locus_tag="IW664_002817"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="IW664_002818"
     tRNA            complement(743..824)
                     /locus_tag="IW664_002818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
BASE COUNT          222 a          242 c          203 g          178 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 caggg
//
LOCUS       DACEKG010000195          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEKG010000195 DACEKG010000000
VERSION     DACEKG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.206"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            27..>793
                     /locus_tag="IW664_002819"
     CDS             27..>793
                     /locus_tag="IW664_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0850103.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
BASE COUNT          284 a          148 c          136 g          225 t
ORIGIN      
        1 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
       61 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      121 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      181 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      241 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      301 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      361 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      421 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      481 ccttaaatga taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc
      541 aaaccgtatc caaagaagcg atttatattg ccattggtat acgagaagac ggcactaaag
      601 aagtactgag ttatgcgatt gctccaactg aatcaacata cgtttggaat gagctgctac
      661 aggatattaa ctccagagga gttcaagaag tcttgctttt tattacggac ggcttaaaag
      721 gcatgaaaga tactatccat caaatttatc ctaaagcaaa atatcagcat tgttgtatcc
      781 atgtatctcg taa
//
LOCUS       DACEKG010000196          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEKG010000196 DACEKG010000000
VERSION     DACEKG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.207"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..782
                     /locus_tag="IW664_002820"
     CDS             <1..782
                     /locus_tag="IW664_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0850104.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DACEKG010000197          775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEKG010000197 DACEKG010000000
VERSION     DACEKG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.208"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(248..772)
                     /locus_tag="IW664_002821"
     CDS             complement(248..772)
                     /locus_tag="IW664_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850105.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          107 c           87 g          319 t
ORIGIN      
        1 gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat
       61 tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag
      121 atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg
      181 atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat
      241 ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta
      301 tatcttctaa attagaagat atttgtatat caatatggaa tatattacct tttttgtatt
      361 tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag
      421 ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt
      481 tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac
      541 ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca aaatctaaac
      601 tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg
      661 gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat
      721 agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc aaacc
//
LOCUS       DACEKG010000198          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEKG010000198 DACEKG010000000
VERSION     DACEKG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.209"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW664_002822"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW664_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0850106.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IW664_002823"
     CDS             complement(481..675)
                     /locus_tag="IW664_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850107.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DACEKG010000199          640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEKG010000199 DACEKG010000000
VERSION     DACEKG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.210"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..625
                     /locus_tag="IW664_002824"
     CDS             <1..625
                     /locus_tag="IW664_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0850108.1"
                     /translation="RDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGY
                     LSQMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNHAHIDQFG
                     EIENANLHVDGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGY
                     HVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          228 a          107 c          127 g          178 t
ORIGIN      
        1 aagagatagt gcaaaaaaat atggaattcc attgactctt gatcaaggaa cttctttatt
       61 tacaaaagga atcatttctc atttgtgggt gacaaattat gtttggggga accacacaga
      121 tccatatggt tacttatcgc aaatgggagt tagcaaagaa aagcttgctt atgatttagc
      181 tcatggattt accgatgaaa atccaactac ttcagatgat aaaccagtca ttgatccaac
      241 tagagcaggt gcagcaaatc ctacgctgac agatggaaca aatcacgccc acattgatca
      301 gtttggagaa atcgaaaacg caaacttgca tgtggatgga tggcacattg ctaattataa
      361 atacgagtat attttcatta tggattacaa tactggaaaa gaattagctc gagtaagagc
      421 tgatggaatt tatagaccag atgtaaatca agcttataat acttctggaa acgttggtta
      481 tcatgtatct ttcaatatgc gtaattttcc taataagaaa gtctatgtaa tgatgcgtgc
      541 aacgaatgat ccagaaggga acactaaagg cggagcacaa gattttcatg ataaacgctg
      601 gtatttaaat attccgcaac gataaaaaaa gcccctcgat
//
LOCUS       DACEKG010000200          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEKG010000200 DACEKG010000000
VERSION     DACEKG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.211"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..278
                     /locus_tag="IW664_002825"
     CDS             <1..278
                     /locus_tag="IW664_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850109.1"
                     /translation="YASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEAEEFVYPF
                     RGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            355..>635
                     /locus_tag="IW664_002826"
     CDS             355..>635
                     /locus_tag="IW664_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0850110.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVP"
BASE COUNT          208 a          114 c          152 g          161 t
ORIGIN      
        1 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
       61 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
      121 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
      181 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
      241 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
      301 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
      361 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
      421 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
      481 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
      541 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
      601 agtcgtcaga tactctacta cagcttctgt gtgcc
//
LOCUS       DACEKG010000201          621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEKG010000201 DACEKG010000000
VERSION     DACEKG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.212"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>621
                     /locus_tag="IW664_002827"
     rRNA            2..>621
                     /locus_tag="IW664_002827"
                     /product="23S ribosomal RNA"
BASE COUNT          196 a          125 c          181 g          119 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg a
//
LOCUS       DACEKG010000202          602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEKG010000202 DACEKG010000000
VERSION     DACEKG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.213"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(318..581)
                     /locus_tag="IW664_002828"
     CDS             complement(318..581)
                     /locus_tag="IW664_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ0850111.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          226 a          104 c           79 g          193 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tctatttttt
      601 ta
//
LOCUS       DACEKG010000203          587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEKG010000203 DACEKG010000000
VERSION     DACEKG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.214"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            26..412
                     /locus_tag="IW664_002829"
     CDS             26..412
                     /locus_tag="IW664_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0850112.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT          221 a           93 c          118 g          155 t
ORIGIN      
        1 ataaaaataa aaaaggagat ctttcatgaa aaaaatttta gggttgatcg ttatcatatt
       61 gattgctttt ataggatgga aaggttggga ctactatcag tctacttatg taggtaaaga
      121 ttattatgcg gtagtcaaag ctccaatgcc tgcagaaact gatatcaaag cagataatgg
      181 ggaagtaatt ggaagaggtt ttaaatacaa cgtagatgct tatgctgaaa acggtgaaaa
      241 aagacagtta gatttcgatg tcattacttc tggtgactct gccaatggtt ctgcttaccc
      301 cgaaggaacg atcttgcagt tgaaagcaag cgaaaagcgg atcatagaga aaaaagtaat
      361 taccagcgat aaggtaccct catctgtaaa agaaaaatta ggcctatctt aagctaagaa
      421 tatatcgcaa aaaagcactc gaagtgagtg cttttttgct tactgattct agaaaacaaa
      481 aaaacagaat taaccttcga agtcagcctc actgttctta aagtcctaaa acaaactaaa
      541 gagcaaggca atcaatcgat ttctatacaa acaagggact gtgacaa
//
LOCUS       DACEKG010000204          543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEKG010000204 DACEKG010000000
VERSION     DACEKG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.215"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..285
                     /locus_tag="IW664_002830"
     CDS             <1..285
                     /locus_tag="IW664_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0850113.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          161 a           94 c          121 g          167 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaagatcaag ccttgaatga gataaattct
      421 tgctcgaaag ttatggaaat ttcgatagcc gtaggccact tgtattgtgc aatagttttt
      481 tacaaaaaaa agggggtgcg gatatttttt tggacctttt taacaaatat atctttgctt
      541 ctt
//
LOCUS       DACEKG010000205          533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEKG010000205 DACEKG010000000
VERSION     DACEKG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.216"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>533
                     /locus_tag="IW664_002831"
     CDS             <1..>533
                     /locus_tag="IW664_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAQ0850114.1"
                     /translation="FRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGSYDRT
                     VKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIADLIEK
                     MYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAIFMDATYIPLKRQTVSKEAIYI
                     AIGIREDGTKEVLSYAIA"
BASE COUNT          165 a          120 c          112 g          136 t
ORIGIN      
        1 ttccgtgtcc atttggaaaa agctgtcaat acgcttctca aaacggagtt aacggctttc
       61 ctcgattacg aaaagtacga tcgcattggt tttaacacgg gtaattctcg taacggctcc
      121 tatgaccgta cggtcaagac cgagtacggg gaacttcatc tccagattcc gcgcgaccgc
      181 aacggcgagt tcaagcaaca gactgttcct gcttatagac ggacgaatga taccttagag
      241 gagaccgtca ttcacctctt ccgaaaaggt attaccatgt cggaaatcgc agacttgatt
      301 gagaaaatgt atgggcatca ctacacgccc caaaccatgt ccaacatgac taaagtgctg
      361 actgaagaag taaatgcatt taaagttaga actctaaatg ataagtatgt cgctattttc
      421 atggatgcta cttatattcc tttaaaacgg caaaccgtct ccaaagaagc aatctatatt
      481 gccattggta tacgagaaga cggcactaaa gaagtactaa gttatgcgat tgc
//
LOCUS       DACEKG010000206          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEKG010000206 DACEKG010000000
VERSION     DACEKG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.217"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>509)
                     /locus_tag="IW664_002832"
     CDS             complement(<1..>509)
                     /locus_tag="IW664_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0850115.1"
                     /translation="DRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNA
                     EIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEP
                     LKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTI
                     SSTLPQSFK"
BASE COUNT          141 a           89 c          116 g          163 t
ORIGIN      
        1 tttaaacgac tgaggtaacg tagatgaaat tgttttaagg gtttcttgaa agccttccgt
       61 acctcgttta acgaaataat agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc
      121 ttcgttgtag cttaacagct catcaacgat ttctgtttga ctaattggtc gtttaaacag
      181 tcgatgataa tgccgtttga gtggctccca tgtcgtggaa tctttgagta agagtttcca
      241 atatcgcttg agccgacggt acttcttctg attttcagag tgtgaggttc gaaaacgatt
      301 catgatctgg acacgtaatt gattgaagct gcgattgatg tgttggacaa tatgaaagcg
      361 gtcagttacg atctccgcat ttggaaagac tgttttaagc aactgacaat agctggcatt
      421 catgtccatc actaggtact tgacctttaa acgggccttt ctaggaaagc gcaggaagta
      481 ttgagttagt ttgtaaagtc tgcgatctt
//
LOCUS       DACEKG010000207          506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEKG010000207 DACEKG010000000
VERSION     DACEKG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.218"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(426..499)
                     /locus_tag="IW664_002833"
     tRNA            complement(426..499)
                     /locus_tag="IW664_002833"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Asn,seq:gtt)
BASE COUNT          163 a          100 c          104 g          139 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa
      241 atcaggtgca tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc
      301 agtgggagca tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta
      361 cactcttagt aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac
      421 ttttactccg gcagtaggac tcgaacctac gacatcatga ttaacagtca tgcgctacta
      481 ccaactgagc tatgccggaa taacat
//
LOCUS       DACEKG010000208          492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEKG010000208 DACEKG010000000
VERSION     DACEKG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.219"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          162 a           95 c           74 g          161 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaacactt
       61 tatacataaa aaaagtcaag tattagaaaa aagttcaaaa gatcaagtgt gcgcttcgct
      121 cccactcccc tcgacgtttt agtagccttt ccctcacttc gttcagtcca agccaactaa
      181 aaagttttcg ggatacattc tccttctctt cctaataatc aattaaaatt ttaatcttta
      241 tatttctgct aatcactcag gaaacagtac agaaaaacaa acacgtatca tagatataaa
      301 tgtaatggca tagtgcgggt tttattttca gcctgtatca tagctaaaca aatcgagttg
      361 tgtgtccgtt tttgggcgtt ctgctagctt gtttaaagtc tcttgaatga atgtatgttc
      421 taagtcaaaa aactctgaca gcgcctttat atagataaag tccgtataat tgtgtaaaag
      481 taaaaaggcc at
//
LOCUS       DACEKG010000209          489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEKG010000209 DACEKG010000000
VERSION     DACEKG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.220"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..414)
                     /locus_tag="IW664_002834"
     CDS             complement(<1..414)
                     /locus_tag="IW664_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850116.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          186 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacata
//
LOCUS       DACEKG010000210          430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEKG010000210 DACEKG010000000
VERSION     DACEKG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.221"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>430
                     /locus_tag="IW664_002835"
     CDS             <1..>430
                     /locus_tag="IW664_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0850117.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DACEKG010000211          427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEKG010000211 DACEKG010000000
VERSION     DACEKG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.222"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..124)
                     /locus_tag="IW664_002836"
     tRNA            complement(53..124)
                     /locus_tag="IW664_002836"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Glu,seq:ttc)
     gene            complement(136..209)
                     /locus_tag="IW664_002837"
     tRNA            complement(136..209)
                     /locus_tag="IW664_002837"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Ile,seq:gat)
     gene            complement(222..292)
                     /locus_tag="IW664_002838"
     tRNA            complement(222..292)
                     /locus_tag="IW664_002838"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Gly,seq:tcc)
     gene            complement(297..369)
                     /locus_tag="IW664_002839"
     tRNA            complement(297..369)
                     /locus_tag="IW664_002839"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Phe,seq:gaa)
BASE COUNT          132 a          113 c           98 g           84 t
ORIGIN      
        1 taaaatcatt ctttcagata aaaagaaaaa gatactcaaa aagtatcttc catgacccgt
       61 acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg
      121 ggccaataaa ataaatgggc ctaaatggac tcgaaccatc gacctcacgc ttatcaggcg
      181 tgcgctctaa ccagctgagc tataggccca taaaaactaa aagcgggtga cgagaatcga
      241 actcgcgaca acagcttgga aggctgtggt tttaccacta aactacaccc gcataatggc
      301 gcgggacaga atcgaactgc cgacacatgg agcttcaatc cattgctcta ccaactgagc
      361 taccgagcca aacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
      421 atgttaa
//
LOCUS       DACEKG010000212          407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEKG010000212 DACEKG010000000
VERSION     DACEKG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.denovo.223"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..384
                     /locus_tag="IW664_002840"
     CDS             <1..384
                     /locus_tag="IW664_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0850118.1"
                     /translation="RKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIE
                     NQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTY
                     NQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           73 c           64 g          113 t
ORIGIN      
        1 cgaaaagaaa tctgtgatga ctttaaggct gtttatcaag ctaactcaaa agaagaagcg
       61 aataccttct tatccggcat gattgagaaa tggaagaaaa actatcctaa agtgacgcag
      121 tcactcatag aaaaccaaga cttattaact ttttatgatt ttccacctag cattcgtaga
      181 accatttact caaccaatct aatcgagtct ttcaataagc aaattaaaag atacagccgt
      241 agaaaagagc agtttcaaaa tgaagaatca ctagaacgct ttctagtcag catttttgat
      301 acatacaatc aaaaatttct aaacagaagc cataaaggtt ttcaacaggt aaccgataca
      361 ttagtttcaa tgtttactga gtaactaatt attttgcagg aggacaa
//
LOCUS       DACEKG010000213        23728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.043, whole genome shotgun sequence.
ACCESSION   DACEKG010000213 DACEKG010000000
VERSION     DACEKG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.043"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            289..564
                     /locus_tag="IW664_002841"
     CDS             289..564
                     /locus_tag="IW664_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850119.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            577..2655
                     /locus_tag="IW664_002842"
     CDS             577..2655
                     /locus_tag="IW664_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0850120.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2815..4701
                     /locus_tag="IW664_002843"
     CDS             2815..4701
                     /locus_tag="IW664_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0850121.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4713..5660
                     /locus_tag="IW664_002844"
     CDS             4713..5660
                     /locus_tag="IW664_002844"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0850122.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5680..6207
                     /locus_tag="IW664_002845"
     CDS             5680..6207
                     /locus_tag="IW664_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0850123.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6270..6923)
                     /locus_tag="IW664_002846"
     CDS             complement(6270..6923)
                     /locus_tag="IW664_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0850124.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7055..7897
                     /locus_tag="IW664_002847"
     CDS             7055..7897
                     /locus_tag="IW664_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0850125.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8055..8951
                     /locus_tag="IW664_002848"
     CDS             8055..8951
                     /locus_tag="IW664_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0850126.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8954..9667
                     /locus_tag="IW664_002849"
     CDS             8954..9667
                     /locus_tag="IW664_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0850127.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9687..10205
                     /gene="msrA"
                     /locus_tag="IW664_002850"
     CDS             9687..10205
                     /gene="msrA"
                     /locus_tag="IW664_002850"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0850128.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10202..10429
                     /locus_tag="IW664_002851"
     CDS             10202..10429
                     /locus_tag="IW664_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0850129.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10581..11132
                     /gene="lepB"
                     /locus_tag="IW664_002852"
     CDS             10581..11132
                     /gene="lepB"
                     /locus_tag="IW664_002852"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0850130.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11215..11757)
                     /locus_tag="IW664_002853"
     CDS             complement(11215..11757)
                     /locus_tag="IW664_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0850131.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11994..12392)
                     /locus_tag="IW664_002854"
     CDS             complement(11994..12392)
                     /locus_tag="IW664_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0850132.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12652..13512
                     /gene="ylqF"
                     /locus_tag="IW664_002855"
     CDS             12652..13512
                     /gene="ylqF"
                     /locus_tag="IW664_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0850133.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13505..14272
                     /locus_tag="IW664_002856"
     CDS             13505..14272
                     /locus_tag="IW664_002856"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0850134.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14328..15185
                     /gene="dprA"
                     /locus_tag="IW664_002857"
     CDS             14328..15185
                     /gene="dprA"
                     /locus_tag="IW664_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0850135.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15307..17385
                     /gene="topA"
                     /locus_tag="IW664_002858"
     CDS             15307..17385
                     /gene="topA"
                     /locus_tag="IW664_002858"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0850136.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17354..18730
                     /gene="trmFO"
                     /locus_tag="IW664_002859"
     CDS             17354..18730
                     /gene="trmFO"
                     /locus_tag="IW664_002859"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0850137.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18928..19833
                     /gene="xerC"
                     /locus_tag="IW664_002860"
     CDS             18928..19833
                     /gene="xerC"
                     /locus_tag="IW664_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0850138.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19870..20418
                     /gene="hslV"
                     /locus_tag="IW664_002861"
     CDS             19870..20418
                     /gene="hslV"
                     /locus_tag="IW664_002861"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0850139.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20432..21832
                     /gene="hslU"
                     /locus_tag="IW664_002862"
     CDS             20432..21832
                     /gene="hslU"
                     /locus_tag="IW664_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0850140.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21850..22641
                     /gene="codY"
                     /locus_tag="IW664_002863"
     CDS             21850..22641
                     /gene="codY"
                     /locus_tag="IW664_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0850141.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22723..23598
                     /locus_tag="IW664_002864"
     CDS             22723..23598
                     /locus_tag="IW664_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0850142.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8212 a         4091 c         4999 g         6426 t
ORIGIN      
        1 tgattcatca gtcccataaa ttatagagcc gatttttgtc gcagccccat ttttttgttg
       61 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
      121 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
      181 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
      241 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
      301 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
      361 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
      421 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
      481 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
      541 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
      601 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
      661 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
      721 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
      781 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
      841 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
      901 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
      961 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     1021 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     1081 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     1141 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     1201 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     1261 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     1321 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     1381 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     1441 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     1501 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     1561 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     1621 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     1681 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     1741 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     1801 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     1861 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     1921 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     1981 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     2041 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     2101 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     2161 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     2221 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     2281 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     2341 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     2401 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     2461 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     2521 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     2581 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     2641 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     2701 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     2761 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     2821 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     2881 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     2941 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
     3001 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
     3061 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
     3121 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
     3181 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
     3241 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
     3301 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
     3361 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
     3421 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
     3481 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
     3541 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
     3601 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
     3661 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
     3721 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
     3781 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
     3841 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
     3901 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
     3961 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
     4021 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
     4081 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
     4141 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
     4201 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
     4261 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
     4321 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
     4381 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
     4441 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
     4501 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
     4561 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
     4621 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
     4681 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
     4741 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
     4801 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
     4861 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
     4921 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
     4981 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
     5041 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
     5101 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
     5161 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
     5221 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
     5281 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
     5341 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
     5401 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
     5461 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
     5521 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
     5581 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
     5641 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
     5701 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
     5761 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
     5821 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
     5881 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
     5941 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
     6001 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
     6061 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
     6121 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
     6181 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
     6241 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
     6301 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
     6361 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
     6421 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
     6481 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
     6541 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
     6601 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
     6661 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
     6721 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
     6781 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
     6841 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
     6901 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa
     6961 tcattatttg tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata
     7021 aggcatcttg ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga
     7081 ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct
     7141 atccatcatg atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt
     7201 tatggaaatg atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga
     7261 attcatctcg atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat
     7321 gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac
     7381 gaaagtaacg gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg
     7441 agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa
     7501 agtacaagac aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg
     7561 tggaagaatc agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat
     7621 gaatttcgcc cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga
     7681 taaatggttt aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg
     7741 tatttcacat gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt
     7801 attcccggac atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg
     7861 taaagacgcg tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag
     7921 cctgaaaggc ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc
     7981 ttttttcata aagactacct ttttgataaa atttttgata acattagaac caatcgaata
     8041 caagaggtga aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat
     8101 taattgcgat catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa
     8161 tgaaaccagt aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag
     8221 ctatcggaga ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc
     8281 cccttgtcgc aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact
     8341 atggtgtaaa tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa
     8401 ttcgaaacaa tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga
     8461 tgaaagttat ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga
     8521 aaaactatca ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg
     8581 cacctattta cattttaggc atctacaatc cattttattt gaactttcct gagatcactg
     8641 acatgcagac aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc
     8701 cgaactgcta ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag
     8761 gtgtcacaga agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca
     8821 aaaataatgt gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga
     8881 tggcaaatgc agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg
     8941 atgatcaata aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa
     9001 ttactggaaa ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt
     9061 aggcagccgt atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga
     9121 aagacaagga gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat
     9181 cagctttttc cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga
     9241 aaatgaagca ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt
     9301 atattttgat ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc
     9361 tatcggaaca ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa
     9421 attaccagat tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca
     9481 attccgaatg caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga
     9541 cattcggttt agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac
     9601 caacaaaact agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga
     9661 tgagtagtat tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct
     9721 ggtgtatgat ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata
     9781 ctggagggca tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata
     9841 cagaagcagt ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac
     9901 tttactggca gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca
     9961 attatcgccc ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta
    10021 agaaacagtt agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag
    10081 cccaaccatt ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa
    10141 attttaaaag aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag
    10201 ggtgaatcga agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc
    10261 tttatctgtt ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag
    10321 ctacgaagaa atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat
    10381 ctttgacgaa gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt
    10441 gtactttatg atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt
    10501 tcagctatct agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa
    10561 gagggatgga ttgatagaga ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg
    10621 tattcaaata cttacttgtt tctgtattcc ttgcgttcat gttgcgggga tttttattca
    10681 tcccggtacc agtggaaggc aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg
    10741 tgatggaaaa attttcggag atcagacgat ttgatattgt tgtttttcaa ttagcagatg
    10801 gcacgattta tatcaaacga gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg
    10861 atcaattgaa gatcaatggg aaagtagtta aggaacctta tctaactaaa aacctgaaat
    10921 cagatcatgc aaatgcatcc tatacgacgg actttacact acaagagcta acgggacaat
    10981 ccaagcttcc ggaagataat tattttgtac ttggagataa tcgaagagtt tcaaaagata
    11041 gtcgttcgtt tggcacgatc aataaaactg atattctagg taaagcaaga tttgtctatt
    11101 atccacttga tgaaataaaa tggatccagt gagaaacaat aaagcgtgca gtctgttgag
    11161 actgcacgct ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac
    11221 agctcgttta aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat
    11281 ttgaataggg atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc
    11341 tgcaccaaac aaaacagaca tcagccaaga attgataaaa aggctgactg ggatcataat
    11401 caataattgg gcagcgatga cggaaggcag gtttatcttt ttatgatata aaaataagcc
    11461 attaataaat ccaccaagga aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc
    11521 aaatatcgtg aaatttaaag tgttcgacaa catttcaagc aaaccagccc agatcggtcc
    11581 atatagataa cccataagcg ccatacttaa aaatccgaat gtgatttgaa aataagcagg
    11641 accaatcgct atcttgtata agatgacatc aagggctaat aaaagagcag aggtcgacaa
    11701 caatcttgca ttcatttcgg gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat
    11761 attctctcct ttgtcagtta ataataaaag gataccataa aatcaccaat gtgtcatgac
    11821 acattggtga tttacataca ctggcatcta acaattaaaa aaagggtgag aagactgctt
    11881 ttataaactg taaaaaagag attgtacgaa taaaaaaaat agaggcggag aatacttatc
    11941 gtatcatccg cctttgtttt tttgttataa aaaacgtcag attagttatc tgcttacatc
    12001 ttcatccata cgggttctaa tgtatttccg tctagatctt ggacttctaa tgaaaacatt
    12061 tcttcttcag gcattcccat atctacttcg tggaaagtac caccattttc ttttgcaatt
    12121 tgagcaaatt tttttacttc atccgcattt tcaacactaa acgaaatcaa agcaccactt
    12181 gtttttttat tatcagctat ttctttatct tttataaatt ttttataaaa atcatgagag
    12241 agaagcatta tccaaaaagt gtcatcccat accattgaac cagcttcttc agtagaaaat
    12301 tcttcatttt ttttaaaacc cagcttttca taaaaagcaa cggaacgatt gacatcagca
    12361 acaggaaaat taacaaaaac catactattc atttgtttcg tctcctttct gttttgttcg
    12421 tctatcatct cttatttagg aaacgctgtc aaaaatttag attcatatat agaaaccaaa
    12481 ataaaaattt gcatcatcaa aaaagtgaag atgtgataga atgaggactg gttcactttc
    12541 acttaactaa tagatggaat ttctttataa taaaaaagtg aatttatatt gaaaaagaaa
    12601 aaaacaagta aagccgggga atattccggt atgaaaggaa tggagagaac aatgaccatc
    12661 caatggtttc cgggacatat ggcaaaagcc cgacgagaag tttctgaaaa aatgaaatat
    12721 gtggacatcg tatttgaatt ggtagacgca cgattacctt tatcttctcg taatccaatg
    12781 ctagatcaga tcattcagca aaaacctcgc cttgttttat tgaataaagc ggatctggca
    12841 gatccacaac agacaaagct gtggcaagaa tattttcgta aacaaggcca tcttgcatta
    12901 gctatcacgg cacaagaaag taaaggaatt aaaggtctag tacctgcggc taaagaagca
    12961 ttgaaagaaa aacgggcaag agatgctgct cgggggatca agcctcgcgc tattcgggca
    13021 atggtcttag gtattccaaa tgttgggaaa tctacgttga tgaatcgact agtaggcaag
    13081 aaaattgcac agacaggaaa taagcctggc gtaacaaaag gtcagcagtg gcttcgttta
    13141 ggtaatgagt tggaattgtt agacacacca gggattttat ggcccaaatt tgaagatgaa
    13201 gaaatcggca aaaaactagc gctgacaggc gcgatcaaag accagcttgt ccatttagat
    13261 gatttagcga tttacggatt agactttttt gcccgatatt attcaggaag aatcagcgat
    13321 cgttatggtt tatcaatcga gcaagaagaa aaattggcac cggaattact gatggatatt
    13381 acagcaaaac gtgggtataa agaagactat gatcgtggca gcgaattagt cgtgcatgat
    13441 attcgtcaag gaaaacttgg acgatacaca ctggatcgtt gcgaagaaat ggagcaaaaa
    13501 agtgatgact gaatccatcc aaagtatcaa agaacgattg aaaagcatac agagaataga
    13561 tgatcctaca ctagaatcat taagaaatga tccacgcaaa ggcgtgcagc aagcactggc
    13621 ttcttttgaa cgacgattga aaaaggaagc attaattaag gaaaaatatg aagaaatgtt
    13681 ttgctttgaa aagcaagggt acgcacaagg tcaccaatgg attgccggca ttgatgaagt
    13741 aggaagagga ccattagctg gacctgtcgt ggcagctgca gttatcttgc cgaaaaatga
    13801 gatcatatta gggctgaatg actccaaaca attatcagag aaaaagcgag aacttcttta
    13861 tcaacaagtt caagaaaaag ccctggcaat cgggatcggg atcgttgatc agcagacaat
    13921 cgatgatgtg aatatttatg aagcaagtaa attagcgatg atccaagcag tagaaaaatt
    13981 gtccattgta ccaagctatt tgttgatcga tgcgatgacc ttggatttgc ctattcagca
    14041 ggaaaaaata atcaaagggg atgcacgatc tgtgtcgatt gctgctgcaa gtatcattgc
    14101 taaagtatat cgagaccaat tgatgaaaga atatgacgtg ctttatcctg gatatggttt
    14161 ctcgcataat gcaggttatg gaacaaagga acacttagaa ggacttgaac gacaaggaat
    14221 tacgccaatc catcgtaaaa gttttgcacc tgtaaaaaaa tatttacaat aaaaaacagc
    14281 cgcttttacg tatcttatta gaaagagaga cgaaaggcgg ttttatgatg tatcaaatag
    14341 aagaaaattt attgaaactt gcttatacaa aaggaatcag tagtcaagga aaatgggcac
    14401 ttgctaactg gatgatgcag tatccttata aagaaatcgg atttgaagac atcattaaga
    14461 taggtaaaat aaccaaacac cgggagctgt ttcgcaaaag ttggcaggaa attcacgcta
    14521 actggggcaa aatccaatcg aaacagtcgt ttatcacttg ctttgatccc aaatatcccc
    14581 ctcagctgct tcacttgacc tatccgccaa ttgtactgtt ttataatggt gatctatctt
    14641 tactttcttg taacatgtta agcgttgttg gtggaagaca cacttctgct ttagccaaaa
    14701 aaacagttta ttatttacta aaaccagtga tagaagctgg atatgtgatc gtcagtggct
    14761 gcgcgaaagg gatagataca tacggtcacc aagcagcaat ctctcatggt ggacggacaa
    14821 tcgcagtcat tggaacaggg atagagcaag tctatcctgc tgaaaatcgt gacttgcaag
    14881 aggtgattgc aaatgatcat cttcttttga gtgaatatcc tcctcaatcc ggcccgaaaa
    14941 agcattgttt tccaatgaga aatcggatta tcgcagcttt gtctaaagga acatgtgtct
    15001 ttgaagccaa gaaaaaaagc ggctctctga taacagcaca gcaagcattg gatcttggaa
    15061 gaacagtttt ttctgttcca ggaaatattt taacagaaca ttctaccgga tgtcatcatt
    15121 tgatccaaga cggtgcagtt tgcacggttt cgggtgaaga tatcttggca gaatttaaag
    15181 aataacacca tacttttcat ttgcttttct tgacaaacgt tttctgaatg gatatgatta
    15241 gctacgattt gtatgaatcc tcttttaata gtaggaacac gacaatggaa aggagtccat
    15301 tcagtaatgg cgtataaata tttagtgatt gtagaatcac ctgcaaaagc caagacaatt
    15361 gagaaatatc ttggacgaaa ctataaagta atggccagtg ttggccatat aagagattta
    15421 ccgaaaagta aaatgggtat cgattttgaa aacaattatg aaccccatta tatttctata
    15481 cgcggaaaag gcgatgtcat caaaagcctg aaagccgcag caaaaaaagc tcaaaaagtt
    15541 tacttggcaa gtgacccgga tagagaagga gaagcgattg cttggcattt agcgtaccta
    15601 cttgggttgg atctgaaaga aaaaaatcgg gtggtcttca atgaaatcac aaaagacgca
    15661 gtcaaagcag cttttaagga accaagaacg atcgatgtag atttagtaga tgcacagcaa
    15721 gctcgtcgta ccttagacag aatcgttggt tattcgatca gtcctattct ctggcgtaag
    15781 gtcaagaaag ggttaagtgc aggacgtgtc caatctgtcg ctttaaaaat tattattgac
    15841 cgtgaaaaag agatccgaga atttgttcca gaagaatatt ggagcatcga cggtaatttt
    15901 aaaaaagctc gcaagaaatt caaagcaaat ttctggggaa tcgacggtaa gaaaaagaaa
    15961 ttaccagatg cacaaagtgt aaaagaagtc actgctagaa ttgatggaac agacttcgaa
    16021 gttaaaaaag tagaaaagaa agagcgcaaa agaaaccctg cgaatccttt cactacaagt
    16081 agcctgcaac aagaagctgc ccgtaagtta aactttcgaa caagaaaaac catgatggta
    16141 gcacagcaat tatatgaagg aatcactttg ggtaaacaag gaacagtcgg cttgattaca
    16201 tacatgcgta ctgattctac ccggattgcc gatacagcaa aagcagaggc cgcagcattt
    16261 attgaagata cgtatggaaa agaattttct agacatgatc accgaaagat caaaaatgct
    16321 caaggcgcac aagatgccca tgaagctgtg cgtccaacga gtgtgcttcg tacaccagat
    16381 gaactcaaac aatatttaga taaagatcaa ttgaaacttt atacattgat ctggtcacgt
    16441 tttgtggcta gtcagatgac accagctatt ttagatacaa tgaaagtgac attagaacaa
    16501 aatggtgtca tcttcattgc gaacggttca aaaatcaaat tcaaaggatt catgcaagtg
    16561 tatgtggaag gtagagatga cggcaaagaa gagaaagaaa atattcttcc agaacttgaa
    16621 gaaggcgatg tagtccaatc tgttgatatt gaaccaaaac aacattttac tcaaccacca
    16681 gcacgtttta gtgaggctac gttgattcgt gcactagaag aaaacggggt agggcgacct
    16741 tccacttatg caccaacact tgatacgatc caaagacgct actatgtaaa attgacacaa
    16801 aaaagatttg agccgactga actaggagaa attgtcaatt cattaatctg tgaattcttc
    16861 cctcaaatcg tcgatatcca tttcacagca gaaatggaag gagatttgga taaaattgaa
    16921 gaaggcacgg aagcgtgggt caaagtggtt gatcgtttct ataaaccgtt tgaaaaagaa
    16981 ctgacgaatg cggaagaaaa aatcgaaaaa attcaaatca aagatgaacc agcaggattc
    17041 gattgtgatg tctgtggaca tccgatggtg ataaaattag ggaaatatgg gaagttttat
    17101 gcttgcagta attttcctga ttgtcggaat acgaaaccaa tcgtaaaaga aatcggtgtg
    17161 acttgcccag tatgtcatga aggacaagtc attgaacgaa aatctaagaa aaacagacta
    17221 ttctatggtt gttcccgttt cccagaatgt gaattcactt cttgggataa accagtgggc
    17281 agaaattgtc caaaatgtga cggatatctg gtcgagaaga aagtcaaagg cggtaaacaa
    17341 gtagtctgtg taaatggaga ttacgaagaa gacatccaaa aataatagaa aagaggttct
    17401 ttcattgaaa atggtaaatg ttgttggagc agggcttgcc ggaagtgaag ctgcttggca
    17461 gatcgcacaa gcaggagtcc ctgtccgttt atatgaaatg cgtccagtca aatcgactga
    17521 ggcgcatcac acaagtaatt ttgctgaact agtatgttcc aattcattaa gaggaaacag
    17581 tttggctaat gcagtagggt tattgaaaga agaaatgcgc cgattgaatt cggtagtaat
    17641 cacttcggca gatgaaacag cagtgccagc tggtggtgcc ttagctgttg accgcgattc
    17701 tttttctgaa acaattaccc gaaaagtcaa agagcatcca ttagtaacag tgataaacga
    17761 agaaattact gaaataccgg aaggaatcac cgtagtagca acaggacctt tgacttctca
    17821 tccattagca gaaagcatca aagcattcaa tggttcagat ggtttttatt tctatgatgc
    17881 agctgcacca atcgtagaca aagctacgat cgatatggat aaagtatatt tgaagtcccg
    17941 ctatgacaaa ggagaagcag cttacttgaa ctgtccgatg acagaagagg aattcaaagc
    18001 tttccatgag gcactaatca atgccgaagt ggcagaactt aaatcttttg agaaagaaaa
    18061 atattttgaa ggatgtatgc caattgaagt aatggcacag cgaggattca aaacgatgct
    18121 tttcggacct atgaaaccag ttggattgga agatcctaaa actggcaaac gtccttatgc
    18181 tgttatccag ctgcgtcaag acaatgctgc agcttcttta tacaacatcg tgggctttca
    18241 gactcacttg aaatggggcg agcaaaaacg tgttttccgg atgattcctg ggttagaaaa
    18301 tgctgaattc gttcgctatg gtgtgatgca ccgaaacagc tttatgaatt cgccagaatt
    18361 gctgaaaccg acttaccaat caacgaaacg cgaagatctg ttctttgcag gtcagatgac
    18421 tggtgtagaa ggctatgttg aaagtgcagc gagcggacta cttgctggaa tcaacgccgc
    18481 acgcttagca aaagaaatgg aaccagtcga gtttccacaa gaaacagcta ttggaagtat
    18541 ggcttactac attactcatg cggaaggaaa acatttccaa ccgatgaatg ccaactatgg
    18601 tctgtttcct gaactgccag agcgcattcg agacaaaaaa gcaagatatg aagcaattgc
    18661 acaacgagct ttaaaagcta atgaggcaat aaaaacagaa ttagaaaaaa ctgcaatccc
    18721 aaatgagtaa ggaaactcca tgaagagggt gagacggata gtctcgctct ttttcttgcg
    18781 tttttcttaa gaatttctca aacaaaatgt gaggaattgc gccaattgtg aacaaactta
    18841 gaaatattgg ttaaattctg acaatttatt tgtcttttta taaacgctgt gctaaattat
    18901 tctctgggac aaaaggaagg tgagaaaatg acagaagcca cgtgggaaca aaaatttttg
    18961 cgttatttga ttgtcgaaag aggatattca gaaaaaacga aagaagcata tgaagaagac
    19021 attctccatt tcaaacattt tttggaggaa tctggagacg ctgatctgct gaaggcggaa
    19081 tattttacgg ttcgtgtcta tttgagtgca ttagctgatc atcgtcctag ctatagcaga
    19141 aattctatca gtcgcaagat agccagccta cgttcgtttt atcagtatct tctcaaagaa
    19201 gaagtgataa aagaaaatcc attttcttac gttcatctca aaaagaaaaa tttgagactg
    19261 ccacgttttt tttatgaaaa tgaaatgcaa gcactttttg acagcgtagc aggagatagt
    19321 cctttggatt tgaggaaccg cgctttactg gaagtccttt acgggtcagg catccgttta
    19381 agtgagtgta gcagtttgtc ggtatcagac attgattttg attcagaggt catgttgatc
    19441 catggtaaag ggaacaaaga gagatatgct cctctaggtt cttttgctca agatgctctg
    19501 caagaatatt tcagggaagg ccgccaggta ttgatgacaa aatatcacga agagcatgat
    19561 atcgtttttg tgaatcatca tggaaaacca atcacgccta ctggaattga atatgtcttg
    19621 aatcaaatga tcaaaaaaag cagtttggat agtgatatcc atcctcacat gctgcgccat
    19681 acatttgcta ctcatttatt aaataatggg gcagatttgc ggacagtcca agaattgcta
    19741 ggacatgcca atttatcgac tacccagatc tatgcccatg taacaaaaga aagtttacaa
    19801 aaaaactatc gttctttcca tccaagagcg taaaaataag gaaaagaaca aatttaggag
    19861 gaaacaaaca tggtcgaatc accatttcat tcaaccacta tctgtgccgt cgaaaaagac
    19921 gggaaatttg ctatggctgg agatggccaa gtaacaatgg gtgaatctgt tgtcatgaaa
    19981 ggcactgcaa agaaagtacg acgtatctac aatggagaag ttgttgtagg tttcgccgga
    20041 agtgttgcag atgcgtttac tttagaagaa aaatttgaag gcaaactaaa cgaatataac
    20101 gggaatctac aacgcgcagc tgtcgaatta gctcaagaat ggcggacaca gcaatctatg
    20161 caaaaacttg aagcgatgct gatcgtaatg aatgataaag aaatgcttct tgtttcaggt
    20221 acaggagaag ttatcacacc agatgatggt atcttagcta tcggatcagg agggaatttt
    20281 gcgttggctg cagcccgagc aatgaaaaac tatgctcaaa acgacatgag tgcgaaagaa
    20341 attgcagaaa atgcgttaaa tattgccgca gatatctgta tctttactaa tcataatatt
    20401 atcgtagaag aactgtaaga ggtgagtaga tatgtatgaa ttaaaacaaa caccaagaca
    20461 aatcgtaagt gaattagatg aatatatcgt aggacagcac gcagcaaaaa aatctgttgc
    20521 cgttgctttg cgtaaccgct atcgccgttt gcagttagat gaaaaaatgc aacaagatat
    20581 tacaccaaaa aatatgctga tgatcggacc tacaggggtc ggtaaaacag aaatcgcacg
    20641 acgtttggca aaaatcgtca atgcaccatt tgtcaaagtc gaagcaacaa aatttactga
    20701 agtcggttat gtcggaagag atgtagaatc aatggttcgg gatttagtag aaaatgcgat
    20761 ccaaatcgta gaaaaagagc agtacagccg tgtatacaca agtgctaaaa aaaatgcaga
    20821 aaaacgtttg gtaaaaatct tggtacctgg tatcaaaaaa gaacagaaac aatccactaa
    20881 tcaatttgaa caaatgatga atctttttaa tgctagtcag caaaataccg ctgaaccgca
    20941 agaagaagtg acagaagata ttcgaatcaa tcgccgtaca gtcttggaac aactacaaaa
    21001 gggtttactg gatagtcgtg aagtaaccat tgaagttgaa gaccaaaaga aacctgcacc
    21061 aatgatgaac aatggattgg agcaaatggg gatcgaccta aatgaaactc taggtgcttt
    21121 gacaccgaag aaaaaagtgg aacggacggt aacagtcaaa gaagctttag agttattgat
    21181 caaggaagaa tcagcaaaat tagtcaagga tgctgatatc catagtgagg caatcaaatt
    21241 agcggaagaa agtggaatta tttttatcga tgaaatcgat aagatcactt ctaaatcaca
    21301 gcaatctgga gaagtatcca gagaaggcgt ccaaagagac atattgccaa ttgtcgaagg
    21361 ttcacaagta aacacaaaat acgggatcat ccaaacagat cacgtcctat tcattgcttc
    21421 aggagctttc catttgtcta aaccaagtga tctaattcca gaacttcaag gacgcttccc
    21481 gatacgggta gaattagatg atttgacagc tgaagatttt gtgaagattc tgaccgaacc
    21541 aaataatgct ttagtaaaac aatacgtcgc gttgatcggt acagaaaatg tgtcagttac
    21601 gtttacaaaa gaagcaattg aacggatcgc tcaaatcgct ttcgatgtca accgtgacac
    21661 agataatatc ggcgcacgca gactgcatac cattttggaa aaactactag aagatctatt
    21721 atttgaagca ccagatatgc aaatgggaga aataacgatc acagaagcat atgtggatga
    21781 caaacttggc tcaattgcgg caaatgaaga tttaagtcgc tatattttat aaaaaatcgg
    21841 aggacgacaa tgacagactt gctaacgaaa acccgcaaga taaacacact tttgcagcaa
    21901 aagaatactt ttgacatgca agcagatttg ccttacgata aaatggctgt cacactaggt
    21961 ggtattcttg acagcaacac ctatatcata ggaagtgacg gagtattact aggttataat
    22021 gaacgtcacg atgtgaacaa tgaacgaatc aaaacaatgt ttgttgagaa acaatttcct
    22081 gaatcttata ctgatacagt ggcacagttg atgaaaactg aagctaatat cccaattact
    22141 agtgatatta ctgcatttcc ggtagagtca agagagaaat atccatttgg tctgacgact
    22201 gtcgttccga tctttggagc tggcgaacgt ttgggtacga ttatcttatc tcgtatggaa
    22261 caatcgtttg cagatgacga tctagtctta gcagaatatg gagctacggt tgtcggtatg
    22321 cagatccttt atcagaagtc tcgaagcatc gaagcagatg tacggagtgc aacggctgtc
    22381 caaatggcaa tcaacacatt atcttatagc gaattgaagg ctgtacaagc tatctttgaa
    22441 gcattggatg gagatgaagg tcgtctgact gcttctaata ttgccgatag tatcggcatc
    22501 actcgttctg tgatcgtcaa tgctttacga aaattggaat cagctggaat cattgaatct
    22561 cgttcgttag gcatgaaggg aacttattta aaagtattga attcccgttt taaagacgag
    22621 ttagcaaaac acagttatta gaagtaaggg gactggggca gatagaaatg tttgttccag
    22681 ccttcccatt ttaaaaagta agtcgaagtt ggaggaatgg caatgactgt tattattcaa
    22741 aatgaccagc tgatcgccga aatcgctgaa cacggtgcgg aattgatcag tctgaaatca
    22801 aaagaacatg gtttagagta tatctggcaa ggtgatccag cgtattgggg cagacatgca
    22861 ccggttttat tcccttttgt tggacgttta aaagatgatc agtatgttta tcaagggaaa
    22921 acatatcaaa tggggcaaca cggatttgct cgggatatgg attttgaagt cattgaacat
    22981 ggacaagaaa tggctagttt catcttaagg agtacgccgg agacattaga aaagtatcca
    23041 tttgattttg aattagtgat cagctatgaa ttaggaggag acggcatcac ggttcattat
    23101 caagtggcaa ataccggtga aaaagaaatg ttcttttcta tcggtggaca tcctgctttc
    23161 aatgtacctc ttgaagaagg attgtcattt gaagatttct atctggcatt ttctccattg
    23221 aaatcaagat tacgcctacc attgaaaggc ccattcatcg atatggatca aaagacgtta
    23281 ggtcagacaa atacgaatct ggctttgaat cgtgaacttt ttgagcaaga tgccttggtt
    23341 tttgaaacaa ggggtctaaa tgcgtacgcc attcgaagtg aaaaatcttc ccatagcgta
    23401 actttaagct ataaagatat gccttatgta gggatctggt ctccttatcc aaaagaagcg
    23461 cctttcgttt gtatcgaacc gtggtgcgga atcgctgaca cggtgaacag tagtggaaac
    23521 ttgatggaaa aagtagggat caatcaacta ggtgcccatg aaatcttcaa gacaaaatat
    23581 tccatcacag tgaagtaaga ggttgtgacc gagaagttag cagggaacac cgtttaccaa
    23641 ggtgtgtaaa aaagctatcc tgtaagtaat aagggagtgt gacatcgatt tgtggctctt
    23701 tgtcaataag gactgatgag ttgtgcaa
//
LOCUS       DACEKG010000214        20863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.050, whole genome shotgun sequence.
ACCESSION   DACEKG010000214 DACEKG010000000
VERSION     DACEKG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.050"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            267..1304
                     /locus_tag="IW664_002865"
     CDS             267..1304
                     /locus_tag="IW664_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0850143.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IW664_002866"
     CDS             complement(1393..1716)
                     /locus_tag="IW664_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0850144.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IW664_002867"
     CDS             complement(1703..2410)
                     /locus_tag="IW664_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0850145.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IW664_002868"
     CDS             2496..2837
                     /locus_tag="IW664_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0850146.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IW664_002869"
     CDS             complement(3008..3856)
                     /locus_tag="IW664_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850147.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IW664_002870"
     CDS             4454..5317
                     /locus_tag="IW664_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0850148.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IW664_002871"
     CDS             5417..5899
                     /locus_tag="IW664_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0850149.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IW664_002872"
     CDS             complement(6162..6629)
                     /locus_tag="IW664_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0850150.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IW664_002873"
     CDS             6752..7603
                     /locus_tag="IW664_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0850151.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IW664_002874"
     CDS             complement(7854..9812)
                     /locus_tag="IW664_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0850152.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IW664_002875"
     CDS             complement(9809..10681)
                     /locus_tag="IW664_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0850153.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IW664_002876"
     CDS             complement(11023..11895)
                     /locus_tag="IW664_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0850154.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IW664_002877"
     CDS             12023..12592
                     /locus_tag="IW664_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0850155.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IW664_002878"
     CDS             12659..13396
                     /locus_tag="IW664_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0850156.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IW664_002879"
     CDS             13627..14895
                     /locus_tag="IW664_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0850157.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IW664_002880"
     CDS             15382..15846
                     /locus_tag="IW664_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0850158.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IW664_002881"
     CDS             15830..16459
                     /locus_tag="IW664_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0850159.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IW664_002882"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IW664_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0850160.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IW664_002883"
     CDS             18338..18784
                     /locus_tag="IW664_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0850161.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IW664_002884"
     CDS             complement(18983..19498)
                     /locus_tag="IW664_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0850162.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20817
                     /locus_tag="IW664_002885"
     CDS             19615..20817
                     /locus_tag="IW664_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0850163.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         6632 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
    19921 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
    19981 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
    20041 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
    20101 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
    20161 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
    20221 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
    20281 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
    20341 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
    20401 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
    20461 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
    20521 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
    20581 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
    20641 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
    20701 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
    20761 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
    20821 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DACEKG010000215        16182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.065, whole genome shotgun sequence.
ACCESSION   DACEKG010000215 DACEKG010000000
VERSION     DACEKG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.065"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..835
                     /locus_tag="IW664_002886"
     CDS             <1..835
                     /locus_tag="IW664_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0850164.1"
                     /translation="DLLDKPTDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCS
                     DGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQG
                     AAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLG
                     AKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPII
                     AICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILP
                     K"
     gene            1093..2595
                     /locus_tag="IW664_002887"
     CDS             1093..2595
                     /locus_tag="IW664_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0850165.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2662..3189
                     /locus_tag="IW664_002888"
     CDS             2662..3189
                     /locus_tag="IW664_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0850166.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3400..4461
                     /locus_tag="IW664_002889"
     CDS             3400..4461
                     /locus_tag="IW664_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0850167.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4464..5360
                     /gene="murQ"
                     /locus_tag="IW664_002890"
     CDS             4464..5360
                     /gene="murQ"
                     /locus_tag="IW664_002890"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0850168.1"
                     /translation="MSKINLENLTTERINQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5374..7356
                     /locus_tag="IW664_002891"
     CDS             5374..7356
                     /locus_tag="IW664_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0850169.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7421..7867)
                     /gene="nrdI"
                     /locus_tag="IW664_002892"
     CDS             complement(7421..7867)
                     /gene="nrdI"
                     /locus_tag="IW664_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0850170.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8078..9502
                     /locus_tag="IW664_002893"
     CDS             8078..9502
                     /locus_tag="IW664_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0850171.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(9683..10456)
                     /locus_tag="IW664_002894"
     CDS             complement(9683..10456)
                     /locus_tag="IW664_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0850172.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10453..11358)
                     /locus_tag="IW664_002895"
     CDS             complement(10453..11358)
                     /locus_tag="IW664_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0850173.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11512..11832)
                     /locus_tag="IW664_002896"
     CDS             complement(11512..11832)
                     /locus_tag="IW664_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0850174.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12306..12992
                     /locus_tag="IW664_002897"
     CDS             12306..12992
                     /locus_tag="IW664_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0850175.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12994..13938
                     /locus_tag="IW664_002898"
     CDS             12994..13938
                     /locus_tag="IW664_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0850176.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            14137..14781
                     /gene="pcp"
                     /locus_tag="IW664_002899"
     CDS             14137..14781
                     /gene="pcp"
                     /locus_tag="IW664_002899"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0850177.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            14860..16137
                     /locus_tag="IW664_002900"
     CDS             14860..16137
                     /locus_tag="IW664_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0850178.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         5213 a         2845 c         3488 g         4636 t
ORIGIN      
        1 agatctactt gataaaccaa cggatcaaac tatccgactt gcttccagtt atgccttagg
       61 agaacttatt aaagatgcac tgcttcaaga agtagaacaa attattgtca ctttaggagg
      121 aagcgggtgt agcgatggtg gtctaggatt actccaatcc ttgggtgctt ctttagaagg
      181 gtacacagaa ggaaatcctt tattatctac taaaaaactt cattttgatc gaaaaaacgt
      241 tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt accaatccat atacaggtca
      301 acaaggagct gcttggcaat ttggaagaca aaaaggagga atagatacga cgctagcttt
      361 cttagataaa caagcaggaa agattgctca gcaaataaaa gatttctggg aggtggattt
      421 agatgatatt ccaggaagtg gagcagcagg cggtattggt ggagcccttt ttttgttggg
      481 agcgaagatg gtttctggtt tcgatttagt cagccaaatc actggattgg aaaaaaagat
      541 cgaacaagca gatgtcatca ttacaggaga agggcggatc gatcagcaaa cgatccacgg
      601 aaaagttcca tacggtgtag cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat
      661 ttgcggaagt agagaccaag agctagaagg actttctgat tttctcccaa ttgtcttaag
      721 tattcatttg ggtccaatag gtttagaaga agcaatggaa cacaggcaaa cattagaaaa
      781 aacaaaaatc ttaggacaga cacttagcag atttttcacc atcctcccga agtagttgac
      841 aaaatcattt tctttatgct atcgtaattt caaatatagg acaaagtgag ggatccgtgc
      901 attgaaaaat taagtcgatt gatgaaagct gcattcagac tgtaccttgt ggagacaagg
      961 aagcagtgtg cagattttcg ggattggctt ggttttttcg atgtatgggt ctttttgtgt
     1021 gtatccaacc cttgcatcct ctttagccag ttctgaaaat aggactggtc tttttttacg
     1081 aggtgatgga aaatgtctaa aatcgaaata aaaaatctga cattcggcta cgacagccaa
     1141 ggcacattat tatttgaaca agcaaatcta aattttgaca cacaatggaa actaggactt
     1201 atcggacgaa acggtcgagg aaagacaact ttactgaata ttctacaaaa caaactacct
     1261 tatcaagggc aagtaatcca tcagcaagaa tttgcctatt tcccgcaaca gacaaaagat
     1321 aaagaacgtt taacctatta cgtgttaaat gatattacgg attttgagat atgggaaatc
     1381 gaaagagagc tccaattgat gcaaacagat cctgaaatct tatggagaga attcagcaca
     1441 ctatcggggg gagagaagac aaaagtccta ctggcacttt tatttgtgga tgacactcat
     1501 ttcccgttaa tcgatgaacc aacgaatcat ttggatatct ctggtagaaa acaagtagcg
     1561 gcttatttga aaaagaaaaa acaaggcttc atcgtggtca gccatgaccg gggatttatc
     1621 gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc aactggaact ttatcaaggg
     1681 aatttctcta tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat
     1741 gaaaaattga aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa agccgaatgg
     1801 tctcgttccc gagaaggaga taaaacaaag aaacaagtcg gattcatcga tactgaatct
     1861 agacgagtga ataaaggagc agtgggtgct gatgctgcac ggacgatgaa acgatccaaa
     1921 gcaatcgtga atcggatgga gacccagatc agcgagaaag aaaaactatt aaaagatatc
     1981 gaatatatcg attctttgac gatgaatagc caagcgtctc accataagcg acttttaagc
     2041 gtagaagatc ttcaattagg gtatgaaaat ctgttattcg agccaattca ttttacaatc
     2101 gagcctcatc agcgggtggc gatttcaggt cctaacggtg caggaaagtc atccattatc
     2161 cattatcttc tgggggcatt caacggcaag gttataggag aaaaatacca gccaaaacat
     2221 ctgagcatta gttatgcaag ccaaaattat gaagacaatc gaggaacgtt ggcggaattt
     2281 gcagagaaaa accaagtaga ctaccaagca tttttgaaca acctccgaaa gcttgggatg
     2341 gaaagagatg tttttcataa caagatcgag cagatgagta tgggccaacg gaaaaaagtg
     2401 gaattggcta aatctttatc acagccagct gaactatata catgggatga accattgaat
     2461 tatttggatg tcttcaatca agaacaatta gaacaactga tcttgaacgt gaaacctgcc
     2521 atgttactag tggaacatga tcaaaccttc ctggataaag tatctactga gattatttct
     2581 cttgagagaa tctaaccctt gtaatctcaa gaacaacaag gtatggttaa ggggaacttt
     2641 gccaacgaag gagagaatca catgctttat gtaacgagac acggagaaac aacttggaac
     2701 gcacaaggat tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg acagatgcag
     2761 gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa aatcatccat
     2821 tcccctcttc aacgagcaag agatacagca caagcagttg ctgatcgtct cagcttgccg
     2881 ctgaccgttg atgaacgatt ggtagaaatg gattttgggg attatgatgg actgccaagc
     2941 aaagatgaga actttcaaaa agcacgttta gcgttcgctg tacgattccc aaatggggag
     3001 tcagtcttag atgtgtatgc acggatcgtt cctttattga aagaatgcat cgaagacgaa
     3061 gaaaatgtct atctgttagt ttgccataat gccttgataa gagtgatcaa tgcttatttt
     3121 catccgatgc ctaatgaggg gttctttaca ttcatggtag ataatacaga attggtttct
     3181 tatgagtaat tatgtttttg tgttatttaa ataaaatatt tcaaggcact gcttttgctt
     3241 agcagtgcct tttttaggtt atgacaattc ttttgataat ataaactcaa cctttagaaa
     3301 tgaaatttaa ttattttaat aaaagtattg aaattttgtt tctttagata tatactctag
     3361 tcatagaaat gaagacggat acagaaaaag agggataata tgtttggctt ttcaatattt
     3421 atgaacgatt ctttgtcaga agagaagaaa cagtatatcc gccaaatggc atccaatggc
     3481 tttgtcggta ttttcacttc catgcatata ccagaagatg atgtttcggc atatcaaaag
     3541 cgattgatcg aactaggaag cgaagcgaaa aagaataaac tagaattgat ggtcgatatt
     3601 tcaggagagg cattggaacg agcgggtttt tcattagaag atctaacgcc tttgaaagca
     3661 attggtgtaa caggtttaag aatggattat catatttcca atcagcagat tgccgactgg
     3721 tcacatcaat tgaaaatcag tttgaatgct agcacgatca cgcctaaaga tatcgatgaa
     3781 ttgaaagaag cagaagctga tttctcacag attgaagctt ggcataatta ttatccacgt
     3841 cccgaaacag ggctggacaa agaatggtat cagaaaaaaa atcaatggct caaaattcaa
     3901 ggtctccttg tccaaggttt tgtaccagga gatacagagt tgagaggccc actatatcaa
     3961 ggattgccaa ctttggaaga acatcgaggg atccatcctc ttgcaggcgc tctagatcta
     4021 ttagcaagta acacggatat tgtttacatc ggagatgcag gtctgtcaga aaacgttcag
     4081 gagcaatttg cttctttcca aaaagaacag acagtcttgc tccatacgga acctgtagat
     4141 gaagaatttt acgaatatat attagggaag catacgaatc ggcaagacga tgcaagagat
     4201 gtcatacgaa gcgtagatgc acgttttcga gagattccgc ctatcccagc aagaaataca
     4261 gctacgcgta tgaaagggtc tattacttta gataacgaaa agtatttgcg ctatatgggt
     4321 gagatccaat taaccaaata tgatttgcca gcggatgaaa aagtgaatgt agtagctaaa
     4381 gttatcaaag aagagctacc attgatcaac caaatcaaag ctggtatgaa ctatcaattt
     4441 ataagaaaag aaggaagata aaaatgagca aaatcaattt agagaaccta acaacggaac
     4501 gcataaacca agaaactttt ggattagatg agatgagtat ttctcaagcc gttcaactaa
     4561 tgaacaaaga agatcagaaa gtggccgctg cggttgaaaa agaactgaca agtatcgaac
     4621 cagtgattgc taacacgatt gagtccttta aaaaaggtgg acgtctgatt tatatgggag
     4681 ccggaacaag tggaagacta ggtgtgttag atgctgctga atgcgtaccg acattcggtg
     4741 tagaaccgga aatggtcata ggtctaattg ctggtggaga agaagcaatg actgtagctg
     4801 tcgaaggggc cgaagattct tatgagctag ggcaagaaga tctaatcaag ctgaatttga
     4861 caaaaaatga tatagttatt ggtatcgcag ctagcggtcg tacaccatat gtgatcggtg
     4921 gattagatta cgcaaaatcg atcggtgctg tcacaggaac gatttcttgc aataaacaag
     4981 ccgaaatcag taaacacgcg gatttcccaa tcgaagtcga tgcaggtcca gaatttttga
     5041 caggctctac tcgattgaag tcaggaacag cacaaaaact tatcttgaat atgatttcta
     5101 ctatcagtat gattggtata ggtaaagtat tcaataactt gatggtcgat gtcaaaccaa
     5161 caaatgaaaa actaatcgaa cgctcaaaac gaatcatcat gcaagcgaca gaagctgatt
     5221 acgaaacagc tgaaaagtac tttgaagaag cagatcaaaa tgttaagctg gctatcgtca
     5281 tgattttgac aaatagtgat aaagaggaag ctcaagcaaa attagctgaa gcaaacggat
     5341 ttgtaaaagg gaccttatga gggggaaaag accatggcaa aaaacgaact gactttggaa
     5401 gaactggcaa aacaaattta taaaggggca ggcggcatgg gcaatgtaga aagcattgtc
     5461 cactgcatga ctcgtgtgcg tatgaagatc atcaatgatg atcttgtaaa tgtctctgaa
     5521 ttaaaagcga ttccaggagt gcttggtgta gtagaagatg aacagcttca agtcatcatc
     5581 ggacctggga aagtgaacaa agtagcacag caaatggttg atttggcagg cgtgaaatta
     5641 ggagaaaaac tttcttctgt aagtacagct acagcaaatt catcagcatc tggaaaagac
     5701 cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa aatcgaaaca aaaaccatct
     5761 aaaatcaaat cggtattaaa agacatttct aatatctttg tgccaatgat tcctgcattt
     5821 gtcggtactg ggatcgtggc agggattgcc gcagttttag caaacttagt cacagccgga
     5881 acacttgatg cagcaacatg gcaacaatat atcgatgtga tgaacatctt gaaaaacggg
     5941 atgtttagct acctagttat ctatacaggt atcaatgctg cacaagtttt tggtgcaaca
     6001 ccaactcttg gtggagttat cggggccgtt gtgatgctga cagggatgaa tccggaagca
     6061 ccactgaaaa acttatttac tggtgaagct cttgcagctg gacaaggtgg gatcctaggt
     6121 gtcatcttcg ctgtttggct attgtcaatc gttgagaaaa aactgcataa agtagtacct
     6181 gatgcggtag atatcatcgt tacaccaact ctttcattga ttgccatcgg tctgattgaa
     6241 attttcttca tcatgccttt agcaggtttc atttctgatg gcatggtcgg tgggatcaac
     6301 tgggtacttg gcgttggtgg agcatttgcc ggcttcgttc taggcacatt attcttgccg
     6361 atggttatgt tcggactgca tcaaattttg actccaatcc atgtgcaaat gattgatgaa
     6421 actggtcgta cgctattgct tccaattctt gcgatggctg gtgcaggaca agtgggtgca
     6481 gcattggcat tatggattcg ctgcaaaaaa gataaagaat taacagaaat gatcaaagga
     6541 gctttaccag tcgggatcct aggaatcggc gaaccattga tttacggagt aacattacct
     6601 ctaggacgcc catttattac tgcttgtatc ggtggaggta tcggcggggc agtgatcgga
     6661 atgatcggaa atgtcggggc tatcgctatt ggaccttcag gagccgcttt gatcccattg
     6721 atctctgacg gtaaatggtt aggctacatc cttggattat tggcagctta tgctggtggc
     6781 ttcgttgcta cattcttctt cggtattcct aaagaccgtt tagaaaaaga agaattagcg
     6841 actgaaacag ccacagtaaa tgaagtgcct gaaaaagtag tacctcaaac ttcagctgaa
     6901 agtgtgacta tcctctcatc agttgctgac ggaacagttg tttctttaga taaagcaagt
     6961 gatccagttt ttgcacaaaa gatgatgggt gaaggctact ttgtagaacc agaaaacgga
     7021 catatctatt ctccagttac tggggtagtc agctctgtct tcccaacgaa acacgctatt
     7081 gggatcacaa cgccaaatgg tttagaaatc ttgcttcata tgggaatcaa cacagttgac
     7141 ttaggcggta cgccatttga tgtgaaagta acagaagggc agcaagtgac acctgatacg
     7201 cttgtagcag aagctgactt agaagcgatc aaagcagcag gcaaagaaac atccatgatc
     7261 gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct tggaaaaaac aggtaaagca
     7321 aaagcaaaag cacctgtgat ggatgtcaaa gcttaatatg atagaaacaa tagaaacgaa
     7381 agaagaaact gaaatctatt tttcagcttc ttctttttta ttatttattg ttttctgcta
     7441 aaatctgata aattcgtgtc acatcagatg gtgttccccg taattcataa tctgccaaga
     7501 aaggaaagcc aaatgtttgt gcatattgtt tggctgttag acaatactga ttattgaagt
     7561 ttttatttcc gctaccgact acaccaagac aaagtttatg attgtcttca tgttccagat
     7621 attcacgcat cgtttcggtc aagatttctg tatcaccgtt atctaaacca tttccgccgt
     7681 ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc tttcgtaaaa tcactatttt
     7741 catggatttc ttttaaagta acttcaggat tgacaggatc ttctgcatgc attttttcag
     7801 catattctgc tagatgtttg gcaaaggcac gcgtatttcc tgaaatcgat atatataaaa
     7861 tgttcatgga tcatcctcca attctttcct tttagtatag catctttttt aagttttttg
     7921 gtggtgtaat ttggaaaggg actaggcgct ttgcttgtat aatgagaaaa aagccggtag
     7981 aattatattt ctattaaagt ttttattaat taagtaaaaa taaaaaaggt ttgttatact
     8041 ttttgataaa agcggtttta ttttggagga tgcttacatg gatacagagc aacagaaaaa
     8101 aggaattggt ttaattccac ttgctgcctt agtaattggt tcagcgattg gaggtggcgt
     8161 atttggtatt atgacggaca tctccggatc ggctggcggc ccagctattt tcagttgggt
     8221 actcgtagga acagcaatgt tgatgctctc cttgtcgatc aataatatca ataaaaaacg
     8281 tccagattta aaaggcggga tctttagtta cgcagaagaa ggttttggcc gtttcgctgg
     8341 ttttatcagc ggttggggtt actggctcac atgctggtta ggcaacgtag cttttgcaac
     8401 attgttgatg agcgcattag gatatttctt cccagtattt gaaggtgggc aaaatctggc
     8461 atcgatcgtg atgagtacag tctttttatg gggatatgca tggctagtga atcggggagt
     8521 cgaaaatgct agtttcgtca atgctatcgt cacattctgt aaattagtgc cattattttt
     8581 atttattgta gtagctatca gcacgttttc gatccaacaa ttcattgata acttttctag
     8641 cgacattata ttgaatgcga atggacagcc gatcccgttt tccagtcaag tattgagttg
     8701 catcatggtg atgctttggg tatttgttgg tattgaagga gcctctgtcg taggttcacg
     8761 agcaaaaaaa caatcagatg ttgggaaagc aacgattcta gggatattcg gcttgattat
     8821 tatttacgtc ttggtttcca tgcttccata tgggacaatg acgatgaatc agctgcaaac
     8881 gatcaaggcg cagcctgcta tgggctatgt gtttgaaatg atggttggta aatggggagc
     8941 agtcgtgatc aacggcggtt tgatcatttc tttagttggt gtttggctct catggacgat
     9001 cttaccgatt gaaacaatgg gaaacatggc tgaagacgga cttttgccta aagcatgggg
     9061 aaaactcaac aaaaatgggg caccaactta tgctatcgta ttgacgacgt tatgtacgaa
     9121 tgtcttttta ctttcattat tattcactga cagtgcttat aactttgctt acacacttgg
     9181 tacagcagct attttcttca cgtggctttt cttaggattg taccaaatga aactttctta
     9241 tcagcggaaa gaatgggtcc aatttttagt agggctttta gcaagtgcct ttcaagtatg
     9301 ggctatgcta gtcgtagcct ttaatgaagt aatgcttgtt cttgttcttt tcttgccagg
     9361 catttatttt tacctcaaag cacgtaagga gcaaaacgag cctgtacaac atatccactt
     9421 agataagatt ttacttatag gtgtgacgat ggtcggagtg attgcaatgg ttttatttgc
     9481 cactgggatg cttcacgtat agaagtataa aagaagtata aagagaggtt gtgagcctgt
     9541 cgtttattca aaaacatact aggaaaggag ctgtgacaca aatgttgtca cagctccttt
     9601 ttcagaataa acggtgttta agagacagcc aatcggcaat aaggctgaac gtctctttca
     9661 cagcctcttt tttgatgagt catcatacat tttttcgctt aaatacgaga tatcctaatg
     9721 ctaagaacaa aacgatgtaa gctaaattac cgaatagcat ttgattagta gaaagatgag
     9781 tcaattgctt catgatctct tcgtttccaa tttggttttg cagattcaac atactgatcg
     9841 gattccattt gatccactcc catttttgga tgagtgcaat aaggatacca gatatgattg
     9901 acgaagcaaa ataaaagacg attcctgctg agatggatgc gccggaacta ttgataaagc
     9961 aagcaatcat caacactaaa cttaaaatca gccataaacc gatataacta cccaaagtat
    10021 aagtgaacaa gctttggatc agtgtttgac cagtagaaac cttctccgtg aaggaaatcg
    10081 aagggaaaag aatcagtttc atcaacacag tgacaataat agctaaaagg tataaataaa
    10141 cagaataaat aactaatgtg atccatttgc ttactaatat ttctcctcga gaatactttc
    10201 gataaaggag atttttcaat gtaccatatt gtgcttccat cgaaatgatc gtgcttgcag
    10261 ctgcaatcat gataaagaca atccaagatg tggcactaaa aagctgagca gtcatttcaa
    10321 ctggatcaat gatcgtcgtg tattttttgg ctagaagtgc tgttcctatg agcaaaacaa
    10381 ctaatagaac agataaaaca gcagttgact ttttcttgat caatttaaat aattcttgtt
    10441 ttacaagcaa tgtcattttt cttcttcccc ctttactgaa tccagaattt ccaatagaga
    10501 agattccaaa tcattcgtca gatgctggac gtctgtcaca tggatatttt gtgcagacaa
    10561 tagctggatc acggttccta ataattcttc gttttctttt aaaagcttga ttttatcagg
    10621 atcttccaaa gtatatccag cttctgtcaa gatcgttcga gcagctagat catttgttgt
    10681 agagataacg atgaaatctt gcgctgtttg gttcaattca gccattgtac attgtttgat
    10741 gatctttcct tgattgatca ggatcagctc atcgaccatt ttttcaagct cgctgagtaa
    10801 gtgactggag atcaagaaag aggtgccctg agtagccaag tcggtaatga tttttcgcac
    10861 atctcttgtt gcttgtggat ccaaaccgtt catcggctca tctaaaataa cgatagacgg
    10921 atggttcagt aaggcaagag caattcccaa tttttgtttc attcccaacg agtaggattt
    10981 cgcttttttg tgaatgtact tttccatctt gagctgattc acgatagtgt cgatagcaga
    11041 tacatcgttg gtttcagaga ataacttcag atgatcatag cctgttaaga atggatagat
    11101 acccgggtat tcgatcaatg ctcctacttg ttccaaccct ttatgactag agggagaaat
    11161 ctcattttca ccaatacaga ttgttccatc tgagtaatgg atcagtccta aaatagcttt
    11221 catgatcgtt gtttttccag cgccattagg acctactaat ccaacgattt tgccagggga
    11281 taagctaaaa ctgacatcgc tgagggcttg tttgtgacca aagttcttgg ataattcttg
    11341 gaccgaaaga cttttcaaaa atattcactc cttttctata gatagtacaa gcatacacct
    11401 gatgagtgga gaagtcgcta ggtcattgga cttatttctt attttttcat aaaaaaattg
    11461 aatattaaat aaaaaggctt attttttctg atcagtactt attccaagtg tctagtggcc
    11521 gctagtaatc ttgatgccaa tgatactgat gagtagcatc cccacaaaga accaagtgga
    11581 aagattcagc ttatcatgaa acaagaccac accaattatg accgaaccaa ctgcgccaat
    11641 ccctgtccag ataggataag agagactcat cggcaaactt ttgattgcct ttgacaaaaa
    11701 gtaaaaacta gcaatcatgc caatcactgt gtaaaaagaa taattgattt tagaaaaacc
    11761 atcactccat ttcatcatcg ttgaccagaa tacttccaaa attcctgcta tgattaattc
    11821 catccatgcc atacttacat tcctccttaa ctataaaaaa atacacaaaa agaggaaaat
    11881 catcccttca aagacctaat ggaatgatca tcctctttat cctacccggt ggcagattgt
    11941 atatcttttt ctgtagtata gcacacctat ccagatatcg gcaatctcat actttttagt
    12001 aaagtgtacc aagcactgac ggatatatcg ttagcggaaa agaagatgaa tgctttgcaa
    12061 tttcatagaa agaagttgtt atttattaat catttaaaaa aaacagtaac tagaccttat
    12121 ttctatatat tatcgggcaa taaattaaaa aaagctttac cttacaataa gtaagataat
    12181 ttttgtgata aaaaaataac gaaaaatatt taaaaatagt gtctatttcg tgagaaaaca
    12241 actatatttt ttatgtgttt tgctttataa ttaaaaaaat attgtcattt tatggaggaa
    12301 aatatatgga aaattatctt agccttttag gcgtcttgat tgttattctc gggtttgcat
    12361 ttaaattaga ctcgatattg atcgtaatgg ttgctttagt tgttacagct ttgactggcg
    12421 gtcttggcat tcaaggaatg ttagaacttt taggtacaag ttttgtaaac aatcgcggga
    12481 tggcgatttt tatcatcatt atgttggcaa caggaacgct tgaaagaaat ggtctgaaag
    12541 aatcagcggc ttccttgatc aagcgattca agaaagtatc tgctggtatg attgtagata
    12601 tttatgggat ctttcgaatg atctttgcgg cttttaacgt gagttttggt ggagttgctg
    12661 gatttgttcg tccaatcatt ttaccgatgg cattggggac catcgaatca aaagggttag
    12721 aagtccatcc taagcatgaa gaagaactaa aaggtatggc ttcagctatg gaaaatgtct
    12781 gctggttctt tggccaagta ctattcatcg gaggagcagg ggcgttgcta gtccaatcaa
    12841 cactgaaaga tctaggatat gacgtaacat tgggacagct tgctttagtg gaagttccag
    12901 tggctatcac ggctttagtt gtggcaagtg tctacttcta tcttaaagaa aagaaattag
    12961 cgaagaaata ttatccagga aaggggcaat aagatggact tttttacaaa tcctgatatt
    13021 ttattaggcg ataaattact ggagatcctt tatatcgtga tgggactcgt attgatttac
    13081 actggagtaa gaaatttaac agataaaacc aatcctcatc gttatgggtc agcctttttc
    13141 tggacggtct taggggtagt tattgcagga ggaagatggc ttccggcaat cgtgaacggt
    13201 ggcttgatct ttgcgatgac cataccagca attgctaaga aagtcagcaa aggagaaagc
    13261 cggcttccat caaaagaata tatggaaaaa atgtcagaca aactaggaat gaaaattttt
    13321 attccagctt tgagtatcgg tgtctttgcg atcttgtttg ctctatttac caatttaggt
    13381 gctttagtcg gcgtgggcgt cggtgttttt gcagccatgc taatcttgat gttcttttct
    13441 cgagataatc gaccaagtac atttttagat gatgcagcag atatgctggg cacagtaggg
    13501 ccgttaagca tgttgccaat gcttttagct tctctaggtg cggtatttac aagcgctgga
    13561 gttgggacag tgatttccaa tgcagtaggt acgatcgttc cagaaggaaa tgtgaacatc
    13621 ggcatcatca tttacgctct tggcatggtg atatttacgg taatcatggg gaatgcattt
    13681 gcggccatca ccgtgatgac ggttgggatc ggtgcaccat tcgtatttag tcatggagcg
    13741 aatccagcgt taataggaat ggttgcattg acttgcggtt tttgtgggac cctgctgaca
    13801 ccgatggctg ctaacttcaa tatcgtgcct gtagcgatgc tggagatgaa agacaaatac
    13861 ggagtgatca aaaaccagtt gtttattgcg ttatttatgc tagttttcca aattgcttat
    13921 atgattctat ttaaataata gaagagaggg gttgtgacag aataattgtc gcaacctctt
    13981 ctaacattgt agtggaacac tattttttta taagaatata ttaagtttga tctgttatgt
    14041 tatttaaact attgtttcct ttatttacgc tgttcaagga aaatggtatg ataaaagata
    14101 accatatgtt aaatcaatcg gacggaggaa caacagatga aagtactagt aacaggtttt
    14161 gatccatttg gtggggacaa agtgaatcca gcctatgaag cagtaaaaaa aatgccagat
    14221 gaaatcagcg gtgctgaaat catcaaagtg gaagtaccga ccgtattcga aaaaagcagt
    14281 caagtagtca aagaagccat ccaacagcac caaccagatg tcgtgatctg tgtcggacaa
    14341 gcaggtgggc ggtcagcagt ctcgttcgaa cgtgtcgcaa tcaatctagc agaagcaaga
    14401 atccctgata atgaaggaaa tcagccattc gatacggctt tagaagaaaa cggtccagcc
    14461 gcttacttta cctcattgcc aatcaaagca atgacaaaaa atgtacaaga tcatggtcta
    14521 ccagcgtaca tctcttatac agcaggaaca tttgtctgca atgatatcat gtatcgtttg
    14581 cttcatttga tcgaaacaca attcccggca attcgaggtg ggttcatcca cgtgtcattc
    14641 tcacccgatc aagtgatcga tcgtccagtg ggtacgcctt ctatgtcttt agaagacatt
    14701 gcttcttctt tgacttatgc agttgaagca gcagtagaaa ataaagaaga tatttccgga
    14761 aatgccggaa caactcacta atttagcaag aaacactggc tggttgctaa cgttatgtta
    14821 gtagccagct ttttgtaatt agaaaggaaa gaaacagccg tggaagaatg gcaatccgtc
    14881 tttgaagaat ggtttccaaa agaaatcagc aaaagctatc ccatcaaaat ttctaaacaa
    14941 tacacaagca gtcaacgttg ggagatctat gcaaaattaa cgaaaaaaca gcgggaacta
    15001 gtcgataagc accgccgata tctaatcagc tctcgtttta tggaagaaca ttatttagct
    15061 gcaacagatt gggtgttttc tgacttcaag atcaatccgt tttttcgaac aaaacgaagc
    15121 cagcaaaaat tgtattgtga gtgtggtaga gaattaaaag tgcaatacat cgttaaatcg
    15181 ccaaaaaccg gaaagatcct aaaactaggc atcaaccact ttgccgatca cctgcatgtc
    15241 tctccgacag tggcagcttc gatccatcag ggaatgacaa aagttgatct ggccttagac
    15301 gaattattat ggcttaagca gaagaatatc gattttcctg aaggactatg gcaaaaatac
    15361 tgttttgtct tgtaccaaaa tcgtcggatg aaacagccat atttaccgga tataaaatta
    15421 gctcagcggc tcgcagaatt tcgccaagta gaaatgccga tttacatagc ggattatcaa
    15481 gcattggaaa atgaaatcaa gaaaataagt gaacacatca atgggcagtc aaaaaagcga
    15541 cagataaaaa aagaactatt tgacgacttt gcagaagaat tagtaaagga tgtagaagaa
    15601 ttcttgatca actatcgcgc atttttacgt aaagattggc aatccatcgt atatgaagaa
    15661 gtgccagtac atcctaatgc ttattttgaa acatttattt ccgtgttgag aaagacaaaa
    15721 cgtcaacgaa caccagaagt cactgctcag atggaatatt ttgcgaaaaa ccaacgattc
    15781 atccaaccaa aaatctatct atttatttgg aagcaatatt gtcgctatgg ttttacagaa
    15841 gggttcttcg acagcattcc tcggatagtc agaaatggtt ttttaaaagt attgcgtaaa
    15901 gaacgagaag ctatacaatc tgctgataag aaagaccgta cagtctcaaa agaaaaatgg
    15961 caactagtag tgaaagatat ccaatctgga aatgtgcaag agacaatcga caaatggaaa
    16021 ggaaaacatt atcgttttac tgaagctcag aaacaagcgt tggaatatta tcagaagtta
    16081 gaagaatcac tgcgtttcaa tgatgaggca agaaagtatc tgaaggaatt actataaaaa
    16141 atagaggctg ggacagaagc gtttaactcc aagaaataag aa
//
LOCUS       DACEKG010000216         8658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.098, whole genome shotgun sequence.
ACCESSION   DACEKG010000216 DACEKG010000000
VERSION     DACEKG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.098"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(565..792)
                     /locus_tag="IW664_002901"
     CDS             complement(565..792)
                     /locus_tag="IW664_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0850179.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(801..1274)
                     /locus_tag="IW664_002902"
     CDS             complement(801..1274)
                     /locus_tag="IW664_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0850180.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2504..3070
                     /gene="amaP"
                     /locus_tag="IW664_002903"
     CDS             2504..3070
                     /gene="amaP"
                     /locus_tag="IW664_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0850181.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3083..3271
                     /locus_tag="IW664_002904"
     CDS             3083..3271
                     /locus_tag="IW664_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0850182.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3284..3841
                     /locus_tag="IW664_002905"
     CDS             3284..3841
                     /locus_tag="IW664_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0850183.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3873..4115
                     /locus_tag="IW664_002906"
     CDS             3873..4115
                     /locus_tag="IW664_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0850184.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4456..5670
                     /locus_tag="IW664_002907"
     CDS             4456..5670
                     /locus_tag="IW664_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0850185.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5701..5919
                     /locus_tag="IW664_002908"
                     /pseudo
     CDS             5701..5919
                     /locus_tag="IW664_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6338..6811
                     /gene="dfrF"
                     /locus_tag="IW664_002909"
     CDS             6338..6811
                     /gene="dfrF"
                     /locus_tag="IW664_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0850186.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6938..7594
                     /locus_tag="IW664_002910"
     CDS             6938..7594
                     /locus_tag="IW664_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0850187.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7776..8306
                     /locus_tag="IW664_002911"
     CDS             7776..8306
                     /locus_tag="IW664_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0850188.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         3031 a         1288 c         1704 g         2635 t
ORIGIN      
        1 ctcgagttac gagtttggaa cagcttttgt ctacagtctg tcattattat caaaataatg
       61 ttaaaaataa aaaagaattg tccatcactg ttattcaaat cattttatca aaactacata
      121 gtaaatgtta aaaagcaaca tgacataata tatagaaggc tttacagtat atatgggagc
      181 ttactaaaaa agaatggagt aagagttaac acaatgggcc attagctgaa gttttaagca
      241 cttcaaggta tgaaacagaa tggcctgtaa tactacatga ttatttagac tgctcaaact
      301 gctatcagtc tggtggaatg aataaagtat tatactaaaa gcagatccaa ctacaacgta
      361 acaggaaacc agttgtatca ggaaaagtga tagtacatgg agaagaaaag gccccctttg
      421 tatgagtcag tacatatctt atttatagat aaaacggtat tcaatcgaaa taaaaaatct
      481 gaataataga cgtaaataac ttctaaaagt ctcacacatc tatcattcag attatttttt
      541 atttactgtt tgttctactt tattttattc ttctggtata gtattcgagt catttttata
      601 ggtcaacaaa tcacctggtt gacactccaa aacttggcag attttttcga gagtggaaaa
      661 acggatggct tttgcttttc cattttttag aatagagatg tttgccatcg taatgcccac
      721 tttctcactc aattcagtca cactcatttt tttttgagct aatactacat ctaaattaac
      781 gataattgcc atgtcgcacc tcacactgtc aaatcatgat ctgtcttaag taaaatcgca
      841 tgttcaagca gtttttccag cacgcttgtt aatgtgtata ctgcaaaagg aatgaatacg
      901 acagcgattc ctaccaacat caaacctgga gcatcgtcta aatccgctac tttcataaat
      961 ttaggaagta agccaaaaaa ggcgaaacca ataaacaaaa tggttcgttc gatttttttt
     1021 agtatgttta agctttgagt ggaaaaagct tggttatctt cgattttttt caatagtttc
     1081 aataagaaat aagcaataac gatccctagg agaagggcaa agtaaatgac agaaacaaat
     1141 aatgtggtct gccaatcaaa attgaatcct ttttcgcttg tagcgatctg agtataaaaa
     1201 agtacgccga acaaaataaa tacggcactt atacctaaaa cgacaaattt taatagcaat
     1261 gttttaaatt tcatttttat ccctctttta tctttgatag ctaaaatata gcagaataat
     1321 tattgtaaaa caactatttt ttatcgaaaa acaataaaaa tatatcgttt ataaaaaatg
     1381 atagagtatg aaggtgtgga gtgcatttaa cagctttttt tgattgtaag aaacatttaa
     1441 aaccagttat tttagattct atgtgtgtct cttctataat gctagtttag atgcctggga
     1501 ttatattgga caatacagtt ggaggatttt ttattatcta gcgaagagaa gtgtaagtta
     1561 acagaactgt atgagaaaaa agacaactac gtagtagcta accgattcat taattttttc
     1621 aagttcatgt ttatttacgt atcagaattt ttcttattca atggtttggt gtaatcacca
     1681 cttatttgtc ctttattgct tatacaatgt tatacaaaag gaaaatcaag cattgacgca
     1741 tggggtggta ctatctagct gaaatagctt aataagcgca agtagatgca ttggattagt
     1801 ttataaatca aaagtgttag aggtattaag aagaagtaga aaaataaaat ggattgccga
     1861 ataaaaagta ccataaatcc taaaacatgt gccaacagat agatggcaca tgtttatttt
     1921 tttcgataat tttgtaacaa aaatacgaag atgaaaaaga atagagattc ccataatgta
     1981 gtagtccaac tgaattcttt actgatacca aataccatat ttcctagatt tgatacaatc
     2041 aaatagccaa tcgttatgat caatgaatag tacaatcttt tcatcatagg acatacctcg
     2101 tttttattta taagaatagt ataaaccaaa gaagtgaata agaaaaatga taatataaaa
     2161 ataaagtaag aacgcattaa agtaactaaa gttatttatt gtatttttta attctccgaa
     2221 aaaatttaat aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt
     2281 aatcttgaat aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa
     2341 gcaattgtta tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg
     2401 gtagcgcttt atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt
     2461 agagttattt acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa
     2521 aataatcggt gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat
     2581 caatgcgccg tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa
     2641 ttactacatg caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat
     2701 catcttgcta gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga
     2761 agatggcaaa ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca
     2821 cgaagaagag ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca
     2881 tatccatgtc aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt
     2941 gatgcaagat atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt
     3001 ggcagtcaca tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg
     3061 agtggaatag gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat
     3121 ctttgggggt cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac
     3181 attgttgatc ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc
     3241 aggaatagtt gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat
     3301 gaatacaaca aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg
     3361 tgaattgact tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt
     3421 agatggtcta ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa
     3481 cacagataac gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga
     3541 tctagatgtg atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga
     3601 attgatcagt actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa
     3661 tgttgcagac atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa
     3721 agtaacagaa gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa
     3781 atctgctgta ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata
     3841 agaagtaaga agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg
     3901 gcggtatcat tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca
     3961 tcgcaaacat cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt
     4021 ggggaccaac aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag
     4081 tattagtcgt ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg
     4141 aaatgaagaa caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt
     4201 tatatgaagt attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa
     4261 ataaacggtt ttctataaaa agaagaccgt ttattttttg tctttatttg tgtgaaaaac
     4321 gaaaaagagc acaacacatc aagcttttat taattgtctg tcttttataa atgcaagtat
     4381 taattcaaaa aaacagaaga aaaaaacaac tacaccttct tttatactcg taatcgtaga
     4441 aaggaggcga aaaaaatgaa tacattacct atcaatacag gagatacagc attcatgatt
     4501 ttatgtacag caatggtttg cctgatgact cctggtctag ctttttttta cggcggatta
     4561 gcacggaaga ataatatact aacgatcatg tctcaatctt ttctctccgt tgggataaca
     4621 acgattatgt gggtatttgg tggattcgga ttggcctttg gacgggatat cggcggtgtt
     4681 attggaaacc cggcagattt ctttttgatg agacatgtta caagcttccc caacatgttt
     4741 catggtgcaa cgattccatt tttaatgttc tttctttacc aactgatgtt tatcgtgatt
     4801 accgttcctt tgatgacagg agcttttgca gggagattga atttaaaagg ctatattata
     4861 cttgttattt tttggaatct gctgatttat ttccctgttt gccactggat atggggtggc
     4921 ggatttttgg atcagatggg atttagagat ttcgctggtg gagcagtcat ccacacgaca
     4981 gctggattcg gctctttagc atgtatcctc acacttggtc aaagaaagat tcctttagga
     5041 gaaagaagtc agcatagcag tcttatggca gctgctatcg gtacaggact gctttggttt
     5101 ggctggttcg gttttaattc cggtggtgca cttcgagcag atgaacaagc agtcaatgca
     5161 tttggttcaa cattcatcgc tcttgcattt gcgatgatta cttggctgat cattgcaaaa
     5221 gtaaaaggaa atggctttga ttttgtcgac gtactaacag gatccgttgc tggattggca
     5281 gccattacgc cttgtgccgg ttatgtagaa gcaaaaagtg ccatgctgat tgggatcatt
     5341 gcgggtatcg tctgtcatgc tgctgttgat tttcgtaaaa agaaacaatg ggatgatgca
     5401 cttgatgtgt ggggtgttca tggcatgggc ggattcacag gtacgattct gatagggatc
     5461 tttgcatcta acagccattt actaacgaat ccaagcagct ggtacttctt agggattcaa
     5521 gtgattggtg tcatcattac tgccgtgtat gcctatgtgc tgacaacagt gattttaaaa
     5581 tcggcgatgc actttacaac gatcactact acaaaagaag aacaagaaga aggattggac
     5641 tttttcttct ttggcaacaa actcaattga aacaaattca aatagaaaag aggaagattc
     5701 atgcaacaaa gcaaaacgaa aattcctgca aaaaaattaa cgttctttgg ctttttggct
     5761 atgaccatct caatggtcgt ttcattatat gaatatccca cattcgcgac ctcaggcttc
     5821 agcttagtat ttttcttgtt attaggcggt ttgctatggt ttatccctgt ggcattgtgc
     5881 gcagcagaga tggcaacggt gaaaggatgg gaaaaaggtg tttcccaaac aaaaagatat
     5941 taagaaacag cccatgttta cctgcttgca aacaaaagaa tattgctact agactaagag
     6001 atactacata agtgtgttgg tatctctttt ttgttgccgt gaaacagcag actatttcat
     6061 ttctgcttga ttaccttaaa acttattagt ctatggtttg tcaagggctt gttgcgtgag
     6121 aaatgctgaa tatcaccctt tacaaaacat aggctgataa gtaacttttc aaaagcagaa
     6181 aagaaatggc aatgcttata acaatatgat ataatgcaga tgaagacaaa aaaggaattt
     6241 gtcgagctgg ttagttcgag ataattttga gtttgttaag atgtggtgca cttgatggaa
     6301 tcacaggtac gacatcatat tatggaggat gaaatagatg ataggtttga ttgttgcgag
     6361 gtcaaagaat aatgttatag gcaagaatgg taatatacca tggaaaataa agggagaaca
     6421 aaagcaattt agagagttaa caacgggtaa tgtggttatt atggggcgaa agtcttatga
     6481 agaaatcggt catccgttgc ctaatagaat gaatattgtt gtttccacca caacagagta
     6541 tcaaggagat aatttagttt cagttaaatc attagaagat gcattattat tggctaaagg
     6601 acgagatgta tacatatctg gtggatatgg actatttaag gaagctttgc aaatagtaga
     6661 taaaatgtat atcacagaag tagatttaaa tattgaagat ggagatacat tctttccaga
     6721 atttgatatc aatgattttg aagttttgat aggggaaaca cttggtgagg aagtgaaata
     6781 tacgagaaca ttttatgtaa ggaaaaaatg aattgagtaa attttggatt tagggtgttt
     6841 tcataaatat attgcttttt atgcatatat aaattgttgt aagacaaatt ccagtttgta
     6901 tggggcgata aaatcagaat ttgagagagg attgaatatg aataaggaat ggtctgaact
     6961 taataaaaca atgcaggcac aaataaaaaa gaaagatact tataagaggg gtattgatac
     7021 tttacttact ttacgaagtc agttaataca aaccttagta tctttcaaag aagaattatg
     7081 cagagaagat tttaactcaa tccccttcat aaacgctgat ggttatcata gtaagacgat
     7141 tgcttattct atttggcata ttttccgcat tgaagatata gttgtgcata cagtgataaa
     7201 tgaagatgaa caagtatttt ttgcaggcaa ttatcaagag cgtatcaatt cacctatcat
     7261 tacaacagga aatgaactca tgaaacagca gattgcagac ttttcaaaac agcttaatct
     7321 ggaagaatta tatttatata ttttcgaagt atgggaaagc actgagaaaa tgcttgaacg
     7381 gttatcttat gatgaattga aaagaaaaat accgaaagaa agaaaaggat acttagaatc
     7441 gttgaatgta gtaaacgaca atgaaaaagc aatttggctg attgattatt ggtgtaataa
     7501 agatatttgt ggactaattc aaatgccgtt ttcaagacat tggattatgc acacagaagc
     7561 ctgtttgcgt ataaagaaca agatacattc ataggcaaaa atttcagttt gtagaactaa
     7621 ggaaattgga atttaaggag gaataaagta tggttagtga ttttataaga gcagcatttc
     7681 catggatttt aatgggttta tttgtagcaa tcagttgttc ccttatgagt aaaaaagaga
     7741 aataaattcc tgtttgtatt tataaaagag aaaaaatgag aaaagtagtt ttatttattg
     7801 ccatgagcct tgacggatat attgcggacg gtaatggtgg agtagcttgg ctaaatggtc
     7861 atggaaatga caatgaaaac atcgacactt atacagaatt taccaaagat atagatactg
     7921 tcttaatggg gtggaatact tctcatcagg ttgttactga actttctccg caagaatggg
     7981 tatataataa atttacaact tatgtattaa cacataaaga gtgcaattct tcccaacaaa
     8041 ttcattttac aagtgaaaat cctgttttat tattggaacg gctaaaacaa gaagcaggaa
     8101 aggatatttg gatttgtggc ggagcaaatc ttgttcagca gttggtaagc aaaaatatga
     8161 ttgataaata ttacatttct gttattccta ctctgttagg aaatggggtt cgtcttttgg
     8221 gcaacataga cagggaaata aagttaaggc tttgtaaaac acagacttat aacggaataa
     8281 ccgatttgat ttatatgcgt agataactag agagaaacag aaaagctgtt aaaccgacgg
     8341 agcgatccga ccacgttggc gggaaaatcc ttaagatttt tccgccatgc ttgcaagggg
     8401 gcggttcgct tcggcggttc ggcgagcttg cgagccgttc aaagcccccg ttatctaaca
     8461 aggggggcac aaaaaacaag aaacactata cactaacaag cggaaaacgc tgtatttata
     8521 aggcaaaacc ccatttttac agtagtgata taaattgtac ctatatcact atgaaaattg
     8581 aatagtaaat atgagaaaga cagttgattt caaaaaatag gctctttgtc aataaggact
     8641 gatgggttgt acaaaatc
//
LOCUS       DACEKG010000217         7959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.105, whole genome shotgun sequence.
ACCESSION   DACEKG010000217 DACEKG010000000
VERSION     DACEKG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.105"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1564
                     /locus_tag="IW664_002912"
     CDS             128..1564
                     /locus_tag="IW664_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0850189.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IW664_002913"
     CDS             1753..4200
                     /locus_tag="IW664_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0850190.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IW664_002914"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IW664_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0850191.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IW664_002915"
     CDS             complement(5690..6793)
                     /locus_tag="IW664_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0850192.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW664_002916"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW664_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0850193.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACEKG010000218         7483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.108, whole genome shotgun sequence.
ACCESSION   DACEKG010000218 DACEKG010000000
VERSION     DACEKG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.108"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            110..193
                     /locus_tag="IW664_002917"
     CDS             110..193
                     /locus_tag="IW664_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0850194.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="IW664_002918"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="IW664_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0850195.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1225..1485
                     /locus_tag="IW664_002919"
                     /pseudo
     CDS             1225..1485
                     /locus_tag="IW664_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1648..2313
                     /locus_tag="IW664_002920"
                     /pseudo
     CDS             1648..2313
                     /locus_tag="IW664_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2349..2594
                     /locus_tag="IW664_002921"
     CDS             2349..2594
                     /locus_tag="IW664_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0850196.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2697..3566
                     /locus_tag="IW664_002922"
     CDS             2697..3566
                     /locus_tag="IW664_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0850197.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3547..4281
                     /locus_tag="IW664_002923"
     CDS             3547..4281
                     /locus_tag="IW664_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0850198.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4314..5226
                     /gene="ant(6)"
                     /locus_tag="IW664_002924"
                     /pseudo
     CDS             4314..5226
                     /gene="ant(6)"
                     /locus_tag="IW664_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5223..5765
                     /gene="sat4"
                     /locus_tag="IW664_002925"
     CDS             5223..5765
                     /gene="sat4"
                     /locus_tag="IW664_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0850199.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5858..6652
                     /locus_tag="IW664_002926"
                     /note="aph(3')-IIIa"
     CDS             5858..6652
                     /locus_tag="IW664_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0850200.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6760..6948)
                     /locus_tag="IW664_002927"
                     /pseudo
     CDS             complement(6760..6948)
                     /locus_tag="IW664_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6928..7470
                     /locus_tag="IW664_002928"
     CDS             6928..7470
                     /locus_tag="IW664_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0850201.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1132 c         1567 g         2161 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccat tttgtgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac tgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttac agatgtaata
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttttaaattt
     1081 ggaaagttac acgttactaa agggaatgga gataaattat tagatatact actgacagct
     1141 tccaagaagc taaagaggtc cctagcgcct acggggaatt tgtatcgata aggggtacaa
     1201 attcccacta agcgctcggg accccttgta ggaaaatgtc ctaagtgtgg caacaatatt
     1261 gtattaaaaa aatcgtttta tggttgttca aattatcctg aatgtaagtt tactttagct
     1321 gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa aagaattact ggagggaaaa
     1381 gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc cctacaatgc cgttgtaaaa
     1441 attggggaaa agggatatat tgattttatc tctttttcaa aataaacatg gggctgaccc
     1501 aaaagtagct cctatatgaa aaatagaggt ctttccattt agatagtagt ggaaagacct
     1561 ctatttatct tttgaaagtg aatacaaaaa taaagcacac tctgctataa tataggtgat
     1621 taatccattt gagagaggtg ctttatcatg tatcaaaatt ataccataaa ccaactttgt
     1681 ttgcctatag atcttgaaat taaattagaa gaaaatgatt ttgctcatgc cattgtgcag
     1741 tttgttgact ctattcccga tgaagtcttt cttccttatt atcaatctat gggtagaccc
     1801 caatatcatc ctcgtatgct actttccatt attctttgtg cctatattca aggggtttat
     1861 tcgggccgcc aaatacaaaa tatgctgata gatagtattc ggatgcgcta cttatctcaa
     1921 gagcaattcc ctaactttcg tactattaat cgttttcgcg ttcatccgat tatgaacgat
     1981 attctagatc attcatttgt tcaatttcgt gagctactgg ttcaaagtgg cttaatcagt
     2041 ggcagtgctc tatacattga tggtacaaaa attgaagcgg atgcgaataa atactctttc
     2101 gtttggaaaa aaagtatttt aaagtataag gatagccttg atcaaaaagc ccttgaaaac
     2161 taccaacaaa tggtggaaac aaaaatctta cctgagttaa tagatgattt aaaggatgaa
     2221 ctttctatag aagagattga aaggattcgt caatctttgg aagaaaagga aaatcaatta
     2281 attgaagcga ttgaccaaac cgaaacagta gaagggggct gacccattat taataaggag
     2341 tggacaacat gattgatgta aatgagctat tggaagaagc tattagagaa actgaaaatc
     2401 taaatgatgg cgaagttttt cttgttaaag atttattcaa gggatatgtg gggaatagga
     2461 tacccagaaa ggatcgactt ctgcttggaa cgttattttt aaaccatgtt agcaaaatga
     2521 atggtaattt aaaggctatt gaaaagactt catctaatca gcagaggtat aaaaagacaa
     2581 ttgataagtt ttaactatct tttgtgaaat taaaaaaatt ataaatatgt tgagtagaag
     2641 caaaagattt ggcttggaat ttttacagca gtgaatcgat aaaaggagca tgcttgatgg
     2701 tagataatat tattaaatca gtagcagaga aattatcctc tctgtcttat atagaaggta
     2761 ttgttttagg tggttcacgt gcaaggggca cccatacaga ggattcggat atagatatcg
     2821 gcatctatta caattcagaa tcatttgaca taaatactat taatcaattc gctacaaagc
     2881 tggatgatga gcatagaaat aaccttgttg tacctcccgg agcatggggt gattggatta
     2941 atggcggcgg atggttagtc ataaacgggt atcatgtgga tttaatttta cgtgatatta
     3001 aacgtgtgga acaaataatg aaagatacag agcacggaat tgttactgcc aattatcaga
     3061 ctgggcatcc ccatggttat attagtgcaa tgtatcgagg agaattagcg attagcaaaa
     3121 tactatatgc taagaatgaa agcttatgcg aattaaaaaa acaggcagaa acttatccca
     3181 atgctttgca gaaaagttta gttaactttt ttatgtttga agcagggttc tctttaatgt
     3241 ttgtaaaagc aaattcggga acagacgata aatattatat tgcgggtcat gtttttcgta
     3301 tagtttcatg tttaaatcaa gtgttatttg catgtaataa tgcttattgt atcaacgaaa
     3361 agaaagctat aaaactgctt gaaacttttg aacataaacc tgaaaaatat accgagaagg
     3421 taaatcatat ttttgaagta ctcggtatct cactttttga atgctacgac atgaccgaga
     3481 agctttataa tgaagtgaat gaaattgtat cggagataaa taacttttta aacgaggaga
     3541 gttcagatga aagaaaacaa atatgatgat aatatatttt ttcaaaaata cagtcaaatg
     3601 agtcgctcgc agaaaggact ggctggtgcg ggagaatggg agactttgaa aaagatgcta
     3661 cctgatttta agggtaagcg tgtgcttgat ttaggatgcg gctatggatg gcactgtata
     3721 tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg atatttctca taaaatgctc
     3781 gaagtagcaa aaggaaaaac ccattttcca cagattgaat atgaatgctg tgccatagaa
     3841 gatgtggatt tcccagagga gagctttgat gtaatactaa gttcgcttgc gtttcattat
     3901 gtagcagact atgagaattt aataaaaaag atatatagga tgctgaaggc tggtggcaat
     3961 ttagttttta cagttgaaca tcctgttttt actgctcatg gaacacaaga ctggtattat
     4021 aacgaaaaag gagaaatact gcatttcccg gtggacaatt attattatga gggcaaacgg
     4081 acagctatgt ttttggaaga aaaggttaca aaatatcata gaacactgac cacatatcta
     4141 aatacactgc tttcaaatag ttttataata aatcagattg tggagccaca gccgccagag
     4201 aacatgatgg atattccggg gatggcggat gaaatgcgac gcccaatgat gctgattgta
     4261 tcggcaaaaa agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat
     4321 cagaaaaaga aatgatggat ttagtacttt ctttagcaga acaggatgaa cgaacgtatt
     4381 cgaattgtga cccttgaggg gtcacgcgca aatattaata tacctaaaga tgaatttcag
     4441 gattatgata ttacatattt tgtaagtgat atagaaccgt ttatatctaa tgatgactgg
     4501 cttaatcaat ttgggaatat aataatgatg caaaagccgg aggatatgga attattccca
     4561 cctgaagaaa agggattttc ctatcttatg ctatttgatg attacaataa aattgatctt
     4621 accttattgc ccttggaaga gttagataat tacctaaagg gcgataaatt aataaaggtt
     4681 ctaattgata aagattgtag aattaaaagg gacatagttc cgactgatat agattatcat
     4741 gtaagaaagc caagcgcaag ggagtatgat gattgctgca atgaattttg gaatgtaaca
     4801 ccttatgtta ttaaaggatt gtgccgtaag gaaattttat ttgctattga tcattttaat
     4861 cagattgttc gccatgagct gctgagaatg atatcatgga aggtcggcat cgaaacaggc
     4921 tttaaattaa gtgtaggcaa gaactataag tttattgaaa ggtatatatc cgaggatttg
     4981 tgggagaaac ttttgtccac ctaccggatg gattcctatg aaaacatatg ggaagcatta
     5041 tttctatgcc atcaattgtt cagggcggta tccggtgagg tggcggaaag gcttcattat
     5101 gcctatccgg agtatgatag gaatataaca aaatatacca gggacatgta taaaaaatac
     5161 actggtaaaa ccggctgcct ggatagcaca tatgccgctg atatagaaga gaggcgggaa
     5221 cagtgattac agaaatgaaa gcagggcacc tgaaagatat cgataaaccc agcgaaccat
     5281 ttgaggtgat aggtaagatt ataccgaggt atgaaaacga gaattggacc tttacagaat
     5341 tactctatga agcgccatat ttaaaaagct accaagacga agaggatgaa gaggatgagg
     5401 aggcagattg ccttgaatat attgacaata ctgataagat aatatatctt tactaccaag
     5461 acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg gaaccggtac gcttatatag
     5521 aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata
     5581 tatctataga atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca
     5641 ataaccttat agcttgtaaa ttctatcata attgtggttt caaaatcggc tccgtcgata
     5701 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
     5761 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
     5821 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
     5881 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     5941 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
     6001 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     6061 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     6121 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
     6181 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     6241 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
     6301 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
     6361 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
     6421 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
     6481 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
     6541 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
     6601 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
     6661 tttagatgtc taaaaagctt taactacaag ctttttagac atctaatctt ttctgaagta
     6721 catccgcaac tgtccatact ctgatgtttt atatcttttc taaaagttcg ctagatagag
     6781 ttctatatta gagatgtaaa gagttttggt gaagaggttt gtgagtatgg cttttataac
     6841 cagggtgcaa tgagaagcac taacagcagc tagcttaaga acgcatagga tatacttttt
     6901 atggaagtca cagaggagga ataaaatttg aacaggatta acagagtaac ctctattctt
     6961 attcagctgc aatcaaaaaa aataattcct gccaaagaaa ttgcacagcg atttaatata
     7021 agcttaagga cagtttacag agatatccgg acacttgaag aagccggaat accaattgga
     7081 tctgaggccg gaaaaggata ttttcttgta gagggcttcc tacttccgcc ggtaatgttt
     7141 acagcggcgg aagtgggtgc attgattaca gcagggaaat ttttaaattg ccatggagat
     7201 gaatcattta taaaggattt tgattcagct atgtataaaa tcaaatctat tttgaagcat
     7261 ggtgaaaaaa actatgcaca ggagttggag aacagtatta acgtgtacag cacatctgga
     7321 cagaaaaata cattggcgga taacgttatt gcagcaattc agactgcaat ctgcaataaa
     7381 agggtaatat caattcaata tcctgcatca ggagggcaag aaccggaaag cagaatgata
     7441 gagccggttc tgttgcaaag ttttaaataa agaataaaat ccc
//
LOCUS       DACEKG010000219         6071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.119, whole genome shotgun sequence.
ACCESSION   DACEKG010000219 DACEKG010000000
VERSION     DACEKG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.119"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..95
                     /locus_tag="IW664_002929"
     CDS             24..95
                     /locus_tag="IW664_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
                     /protein_id="HAQ0850202.1"
                     /translation="MIEPKNPSDKSIYHWDFYALLGF"
     gene            111..2030
                     /gene="tet(M)"
                     /locus_tag="IW664_002930"
     CDS             111..2030
                     /gene="tet(M)"
                     /locus_tag="IW664_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0850203.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2128..3501
                     /gene="tet(L)"
                     /locus_tag="IW664_002931"
     CDS             2128..3501
                     /gene="tet(L)"
                     /locus_tag="IW664_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0850204.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            4065..5327
                     /locus_tag="IW664_002932"
     CDS             4065..5327
                     /locus_tag="IW664_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0850205.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5572..6054
                     /locus_tag="IW664_002933"
     CDS             5572..6054
                     /locus_tag="IW664_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0850206.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         2076 a          875 c         1254 g         1866 t
ORIGIN      
        1 attctgattc atcagtccca taaattatag agcctaaaaa tcccagtgat aagagtattt
       61 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
      121 ttaatattgg agttttagct catgttgatg cgggaaaaac taccttaaca gaaagcttat
      181 tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg
      241 ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc
      301 agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag
      361 aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg
      421 gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa
      481 tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata
      541 ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata
      601 tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg
      661 accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg
      721 aaagcataag atttcagaat tgctccttgt accctgttta tcatggaagc gcaaaaagca
      781 acatagggat tgagcagctt atagaagtga taacgaataa attttattca tcaacataca
      841 gaaagaagtc tgaactttgc ggaaatgtct tcaaaattga atattcggaa gaaagacaac
      901 gtcttgcata tgtacgcctt tatggcggaa tcctgcattt gcgggattcg gttagaatat
      961 cggaaaagga aaaaataaaa attacagaaa tgtatacttc aataaatggt gaattatgta
     1021 aaattgataa ggcttattcc ggggaaattg ttattttgca aaatgagttt ttgaagctaa
     1081 atagtgttct tggagataca aagctattgc cacagagaga gagaattgaa aatccgctcc
     1141 ctctgctgca aacaactgtt gaaccgagca aacctcaaca aagggaaatg ttacttgatg
     1201 cacttttaga aatctccgac agtgacccgc ttctacaata ttatgtggat tctacgacac
     1261 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
     1321 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
     1381 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
     1441 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
     1501 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
     1561 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtatg
     1621 gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct cctattgtat
     1681 tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt agttttaaaa
     1741 tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa tattgtgcga
     1801 acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa atccctgctc
     1861 ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt agtgtttgtt
     1921 taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag ccccgtcgtc
     1981 caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag tgtattttat
     2041 gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa taaagatttg
     2101 aattaaagtg taaaggagga attaattatg aagtgtaatg aatgtaacag ggttaaatac
     2161 aaccaaattt taatttggct ttgcgtttta tcttttttta gcgtattaaa tgaaatggtt
     2221 ttgaacgtct cattacctga tattgcaaat gattttaata aaccacctgc gagtacaaac
     2281 tgggtgaaca cagcctttat gttaaccttt tccattggaa cagctgtata tggaaagcta
     2341 tctgatcaat taggcatcaa aaggttactc ctatttggaa ttataataaa ttgtttcggg
     2401 tcggtaattg ggtttgttgg ccattctttc ttttccttac ttattatggc tcgttttatt
     2461 caaggggctg gtgcagctgc atttccagca ctcgtaatgg ttgtagttgc gcgctatatt
     2521 ccaaaggaaa ataggggtaa agcatttggt cttattggat cgatagtagc catgggagaa
     2581 ggagtcggtc cagcgattgg tggaatgata gcccattata ttcattggtc ctatcttcta
     2641 ctcattccta tgataacaat tatcactgtt ccgtttctta tgaaattatt aaagaaagaa
     2701 gtaaggataa aaggtcattt tgatatcaaa ggaattatac taatgtctgt aggcattgta
     2761 ttttttatgt tgtttacaac atcatatagc atttcttttc ttatcgttag cgtgctgtca
     2821 ttcctgatat ttgtaaaaca tatcaggaaa gtaacagatc cttttgttga tcccggatta
     2881 gggaaaaata taccttttat gattggagtt ctttgtgggg gaattatatt tggaacagta
     2941 gcagggtttg tctctatggt tccttatatg atgaaagatg ttcaccagct aagtactgcc
     3001 gaaatcggaa gtgtaattat tttccctgga acaatgagtg tcattatttt cggctacatt
     3061 ggtgggatac ttgttgatag aagaggtcct ttatacgtgt taaacatcgg agttacattt
     3121 ctttctgtta gctttttaac tgcttccttt cttttagaaa caacatcatg gttcatgaca
     3181 attataatcg tatttgtttt aggtgggctt tcgttcacca aaacagttat atcaacaatt
     3241 gtttcaagta gcttgaaaca gcaggaagct ggtgctggaa tgagtttgct taactttacc
     3301 agctttttat cagagggaac aggtattgca attgtaggtg gtttattatc cataccctta
     3361 cttgatcaaa ggttgttacc tatggaagtt gatcagtcaa cttatctgta tagtaatttg
     3421 ttattacttt tttcaggaat cattgtcatt agttggctgg ttaccttgaa tgtatataaa
     3481 cattctcaaa gggatttcta aatcgttaag ggatcaactt tgggagagag ttcaaaattg
     3541 atcctttttt tataacagga attcaaatct ttttgttcca ttaaagggcg cgattgctga
     3601 ataaaagata cgagagacct ctcttgtatc ttttttattt tgagtggttt tgtccgttac
     3661 actagaaaac cgaaagacaa taaaaatttt attcttgctg agtctggctt tcggtaagct
     3721 agacaaaacg gacaaaataa aaattggcaa gggtttaaag gtggagattt tttgagtgat
     3781 cttctcaaaa aatactacct gtcccttgct gatttttaaa cgagcacgag agcaaaaccc
     3841 ccctttgctg aggtggcaga gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga
     3901 aaggtcttaa aggttttatg gttttggtcg gcactgccga cagcctcgca gagcacacac
     3961 tttatgaata taaagtatag tgtgttatac tttacttgga agtggttgcc ggaaagagcg
     4021 aaaatgcctc acatttgtgc cacctaaaaa ggagcgattt acatatgagt tatgcagttt
     4081 gtagaatgca aaaagtgaaa tcagctggac taaaaggcat gcaatttcat aatcaaagag
     4141 agcgaaaaag tagaacgaat gatgatattg accatgagcg aacacgtgaa aattatgatt
     4201 tgaaaaatga taaaaatatt gattacaacg aacgtgtcaa agaaattatt gaatcacaaa
     4261 aaacaggtac aagaaaaacg aggaaagatg ctgttcttgt aaatgagttg ctagtaacat
     4321 ctgaccgaga tttttttgag caactggatc caggagaaca aaaacgattt tttgaggaaa
     4381 gttataaatt attttccgaa cgatatggca agcaaaatat tgcttatgca acagttcata
     4441 atgatgagca aacccctcac atgcatttag gtgttgtgcc tatgcgtgat ggaaaactgc
     4501 aaggaaaaaa tgtgtttaat cgtcaagaac tgttatggct acaagataaa ttccccgagc
     4561 atatgaaaaa acagggtttt gagttgaagc gtggtgaacg tggctctgac cgtaaacata
     4621 ttgagacagc taaatttaaa aaacaaactt tggaaaaaga gattgatttt ctagaaaaaa
     4681 atttagcagt taaaaaagat gaatggactg cttatagcga taaagttaaa tcagatttag
     4741 aagtaccagc gaaacgacac atgaaaagtg ttgaagtgcc aacgggtgaa aagtccatgt
     4801 ttggtttggg aaaagaaata atgaaaacag aaaagaaacc aaccaaaaat gttgttatat
     4861 cggagcgtga ttataaaaac ttagtgactg ctgcgagaga taacgatagg ttaaaacagc
     4921 atgttagaaa tctcatgagt actgatatgg cgagagaata taaaaaatta agtaaagaac
     4981 atgggcaagt taaagaaaaa tatagtggtc ttgtagagcg atttaatgaa aatgtaaatg
     5041 attataatga gttgcttgaa gaaaacaagt ctttaaagtc taaaataagc gatttaaagc
     5101 gtgatgtgag tttaatctat gaaagcacta aggaattcct taaggaacgt acagacggct
     5161 taaaagcctt taaaaacgtt tttaaggggt ttgtagacaa ggtaaaggat aaaacagcac
     5221 aattccaaga aaaacacgat ttagaaccta aaaagaacga atttgaacta actcataacc
     5281 gagaggtaaa aaaagaacga agtcgagatc agggaatgag tttataaaat aaaaaaagca
     5341 cctgaaaagg tgtctttttt tgatggtttt gaacttgttc tttcttatct tgatatatat
     5401 agaaataacg tcatttttat tttagttgct gaaaggtgcg ttgaagtgtt ggtatgtatg
     5461 tgttttaaag tattgaaaac ccttaaaatt ggttgcacag aaaaacccca tctgttaaag
     5521 ttataagtga ccaaacaaat aactaaatag atgggggttt cttttaatat tatgtgtcct
     5581 aatagtagca tttattcaga tgaaaaatca agggttttag tggacaagac aaaaagtgga
     5641 aaagtgagac catggagaga aaagaaaatc gctaatgttg attactttga acttctgcat
     5701 attcttgaat ttaaaaaggc tgaaagagta aaagattgtg ctgaaatatt agagtataaa
     5761 caaaatcgtg aaacaggcga aagaaagttg tatcgagtgt ggttttgtaa atccaggctt
     5821 tgtccaatgt gcaactggag gagagcaatg aaacatggca ttcagtcaca aaaggttgtt
     5881 gctgaagtta ttaaacaaaa gccaacagtt cgttggttgt ttctcacatt aacagttaaa
     5941 aatgtttatg atggcgaaga attaaataag agtttgtcag atatggctca aggatttcgc
     6001 cgaatgatgc aatataaaaa aattaataaa aatggttctg ttgcaaagtt ttaaataaag
     6061 aataaaatcc c
//
LOCUS       DACEKG010000220         4995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.129, whole genome shotgun sequence.
ACCESSION   DACEKG010000220 DACEKG010000000
VERSION     DACEKG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.129"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..2180)
                     /locus_tag="IW664_002934"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IW664_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IW664_002935"
     CDS             2326..2901
                     /locus_tag="IW664_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0850207.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IW664_002936"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IW664_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0850208.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IW664_002937"
     CDS             3788..4942
                     /locus_tag="IW664_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0850209.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACEKG010000221         4782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.132, whole genome shotgun sequence.
ACCESSION   DACEKG010000221 DACEKG010000000
VERSION     DACEKG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.132"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            281..1192
                     /locus_tag="IW664_002938"
     CDS             281..1192
                     /locus_tag="IW664_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0850210.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1345..1830
                     /gene="vanZ"
                     /locus_tag="IW664_002939"
     CDS             1345..1830
                     /gene="vanZ"
                     /locus_tag="IW664_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0850211.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2117..2446)
                     /locus_tag="IW664_002940"
     CDS             complement(2117..2446)
                     /locus_tag="IW664_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0850212.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2751..3080
                     /locus_tag="IW664_002941"
     CDS             2751..3080
                     /locus_tag="IW664_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0850213.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3070..3357
                     /locus_tag="IW664_002942"
     CDS             3070..3357
                     /locus_tag="IW664_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0850214.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3636..4199)
                     /locus_tag="IW664_002943"
     CDS             complement(3636..4199)
                     /locus_tag="IW664_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0850215.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1680 a          721 c          813 g         1568 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg aggggtggta tttcacaccg
       61 cccattgtca acaggcagtt cagcctcgtt aaattcagca tgggtatcac ttatgaaaat
      121 tcatctacat tggtgataat agtaaatcca gtagggcgaa ataattgact gtaatttacg
      181 gggcaaaacg gcacaatctc aaacgagatt gtgccgttta aggggaagat tctagaaata
      241 tttcatactt ccaactatat agttaaggag gagactgaaa atgaagaagt tgtttttttt
      301 attgttattg ttattcttaa tatacttagg ttatgactac gttaatgaag cactgttttc
      361 tcaggaaaaa gtcgaatttc aaaattatga tcaaaatccc aaagaacatt tagaaaatag
      421 tgggacttct gaaaataccc aagagaaaac aattacagaa gaacaggttt atcaaggaaa
      481 tctgctatta atcaatagta aatatcctgt tcgccaagaa agtgtgaagt cagatatcgt
      541 gaatttatct aaacatgacg aattaataaa tggatacggg ttgcttgata gtaatattta
      601 tatgtcaaaa gaaatagcac aaaaattttc agagatggtc aatgatgctg taaagggtgg
      661 cgttagtcat tttattatta atagtggcta tcgagacttt gatgagcaaa gtgtgcttta
      721 ccaagaaatg ggggctgagt atgccttacc agcaggttat agtgagcata attcaggttt
      781 atcactagat gtaggatcaa gcttgacgaa aatggaacga gcccctgaag gaaagtggat
      841 agaagaaaat gcttggaaat acgggttcat tttacgttat ccagaggaca aaacagagtt
      901 aacaggaatt caatatgaac catggcatat tcgctatgtt ggtttaccac atagtgcgat
      961 tatgaaagaa aagaatttcg ttctcgagga atatatggat tacctaaaag aagaaaaaac
     1021 catttctgtt agtgtaaatg gggaaaaata tgagatcttt tattatcctg ttactaaaaa
     1081 taccaccatt catgtgccga ctaatcttcg ttatgagata tcaggaaaca atatagacgg
     1141 tgtaattgtg acagtgtttc ccggatcaac acatactaat tcaaggaggt aaggatggcg
     1201 gaatgaaacc aacgaaatta atgaacagca ttattgtact agcacttttg gggtaacgtt
     1261 agctttttaa tttaaaaccc acgttaacta ggacattgct atactaatga tacaacttaa
     1321 acaaaagaat tagaggaaat tatattggga aaaatattat ctagaggatt gctagcttta
     1381 tatttagtga cactaatctg gttagtgtta ttcaaattac aatacaatat tttatcagta
     1441 tttaattatc atcaaagaag tcttaacttg actccattta ctgctactgg gaatttcaga
     1501 gagatgatag ataatgttat aatctttatt ccatttggct tgcttttgaa tgtcaatttt
     1561 aaagaaatcg gatttttacc taagtttgct tttgtactgg ttttaagtct tacttttgaa
     1621 ataattcaat ttatcttcgc tattggagcg acagacataa cagatgtaat tacaaatact
     1681 gttggaggct ttcttggact gaaattatat ggtttaagca ataagcatat gaatcaaaaa
     1741 aaattagaca gagttattat ttttgtaggt atacttttgc tcgtattatt gctcgtttac
     1801 cgtacccatt taagaataaa ttacgtgtaa gatgtctaaa tcaagcaatc tgatctttca
     1861 tacacataaa gatattgaat gaattggatt agatggaaaa cgggatgtgg ggaaactcgc
     1921 ccgtaggtgt gaagtgaggg gaaaaccggt gataaagtaa aaagcttacc taacactata
     1981 gtaacaaaga aagcccaatt atcaatttta gtgctgagga attggtctct ttaataaatt
     2041 tccttaacgt tgtaaatccg cattttcctg acggtacccc caaactaaga tttcatagat
     2101 agattttctg ataactttaa aaaacacgaa tagcggaaag gatgtcttca ttcgctaaag
     2161 agtaataaac tacttttcca tcttgacgtg attttgccat tccatgtttt tttaaaaaac
     2221 gtaaatggtg tgacgttgta gcgacactag cagtaagtat ttctgctaaa tcacagacgc
     2281 acatttcctt ataggtttct aaagcataga aaattttaat tctagaaaaa tcgctgaaac
     2341 atttccctaa cacaagtaag tttgaaaaat ctttttgatc caatttattt tttattccat
     2401 ttactttttc tttatgtacg acagtcactt ggcaaacttt tttcattcat ctcacctcac
     2461 attcaaacga ttatttgaat gaagtatacc tatttgatca ctcttttgtc aatctacctt
     2521 aattccaata cactattata agcaatttca tgtattgaag taaacttact tgcaagtgcg
     2581 tttttgattc caagttaaaa acatattatt attctacata atttcctaat caaataaaac
     2641 tcctttgaaa attgttctaa aggagtttta ttattttgta tttctactaa aaatagcgta
     2701 caatgtaatt actaagtaat tacgaaatgt aaaataatgg aggaaataat atgattacaa
     2761 aaacaagaaa acaaggaaat tctattatgt taacggtccc taaagaattc gatgtgccca
     2821 atggtgttga agtagaagct aagcttgtag agaatggtat cctttatgag tttgtagagc
     2881 ctaaaaaaga attttttgat tttagtgaag atattttggc tgatattctt tcagaaggtt
     2941 atagcaaaca agagatctta aaagaattta gaaatagaaa aaatgcgtta acttctgcat
     3001 ttaaatctat tgctgaagat actgttgcaa attctaaacc gatgacaaaa gaggagttgg
     3061 cagcagaaat tggcttatga gattctacct tctaaacacg taataaagta tctaaaaaaa
     3121 ttaaaagaga aaacattaaa agaacaattt ttaacgataa tttatgatga gatagcagtt
     3181 cgtcctcatt cgggagaaca aaaaacgggt gatttatcag gaatttgggc tattggattc
     3241 aagtatgcag gtacgacata tcgcgttgca tatgaaatta aagataatgc agtgatacct
     3301 atcttactat gtggaacgca tgaaaatttt tatgaacaat taaaaaagat taggtaagaa
     3361 cccaatgttt caacttgaga ggatagacga cactttaaaa gataattttt aacagctgag
     3421 agttgtaata ccctataatt ttaaaccact aacctatata tttatgaaaa atagaaaaaa
     3481 ttaaaacgca ttttaagcct ctttcaaatt aaaagcttca tccttatgca caaagagggc
     3541 ttaaaatgcg ttattttata ttgtttttta gtaataataa aaaaatcact ctaattctcc
     3601 cataaatatt atagattcct aaagattaaa atagattatt ccctattgtc ttttaagtag
     3661 cggtagagtg ttgccttgct aataccagtc atttccgtaa tttcttttac gctataaacc
     3721 tgacttttat acaattttac tgctcgatca acatctttct ggttcactcg tggacggcca
     3781 cctttacgac ctctcgcacg tgcacttttc aaaccttctt tggtacgttc aacaattaaa
     3841 tcacgttcga actgactaaa tgcttggaag acagtcatca ttaagcgtcc gtgaggcgtg
     3901 ctggtatcga agttttcttt caagctcacc agcttaacat ggtgttcttc aaaataggta
     3961 acaaggtcaa tcaaatcctt tgtactacga cccaatcgag aaaaactttc tacaattacc
     4021 gtatctcccg gacgaatttt atcttttaaa cgatttagtt ggggacgaac cgctttggtt
     4081 cccgtcattt tctcagttag gatttcatta caattttgtt cattgagtaa gtctaattga
     4141 cgatttagtt cttgtgaacg tgtactcact cgggcataac cataaatgta tccactcatg
     4201 cttttcttcc tttcgtactt taattctggc ttgattgtat cataaaaggg agttatagat
     4261 acatagttaa tgcaacaagt tatgatacaa aatatgacct cttttattta ataaaagagg
     4321 tcatttcatt ttgtctcata atcgattgtt tttgatacga gtgtgtatac gaaataaaca
     4381 aaaaaatctt aagtaaattt ctagatacct aatttaatcc taactagtgt atcctaaagt
     4441 taataacgag tttatcacaa tgaaaattat aaaatgctcc catattagat ataacatgca
     4501 atatttgaat tagaactaca aaatttattc gttttataaa gtaaataatg ttgagatact
     4561 aaggaataga atattataag gaattgagct gaatgtattg gataaaaaaa gaataacttt
     4621 attagagatg gactataaaa atgctcgtaa ttttcttctg aagtctgaaa gctatttgaa
     4681 tgctcagcta ccagaatata ttagttttga cgaaacttta aaatctgcta aaaaagtttt
     4741 aacttcgccg tttgtataat taagcgagac aaataaaaaa gc
//
LOCUS       DACEKG010000222         2933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.164, whole genome shotgun sequence.
ACCESSION   DACEKG010000222 DACEKG010000000
VERSION     DACEKG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.164"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            244..1212
                     /locus_tag="IW664_002944"
     CDS             244..1212
                     /locus_tag="IW664_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0850216.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1205..2236
                     /gene="vanA"
                     /locus_tag="IW664_002945"
     CDS             1205..2236
                     /gene="vanA"
                     /locus_tag="IW664_002945"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0850217.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2242..2850
                     /locus_tag="IW664_002946"
     CDS             2242..2850
                     /locus_tag="IW664_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0850218.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          871 a          567 c          706 g          789 t
ORIGIN      
        1 tgctggtgtc atccaccaac atcaaaaagt atagagccat aattttttag gaaaatctca
       61 aggttatctt tactttttct taggaaatta acaatttaat attaagaaac ggctcgttct
      121 tacacggtag acttaatacc gtaagaacga gccgttttcg ttcttcagag aaagatttga
      181 caagattacc attggcatcc ccgttttatt tggtgccttt cacagaaagg gttggtctta
      241 attatgaata acatcggcat tactgtttat ggatgtgagc aggatgaggc agatgcattc
      301 catgctcttt cgcctcgctt tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa
      361 tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg tgggacataa atcagagatt
      421 tccgcctcta ttcttcttgc gctgaagaga gccggtgtga aatatatttc tacccgaagc
      481 atcggctgca atcatataga tacaactgct gctaagagaa tgggcatcac tgtcgacaat
      541 gtggcgtact cgccggatag cgttgccgat tatactatga tgctaattct tatggcagta
      601 cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac
      661 cgtggcaagg tactcagcga catgacagtt ggtgtggtgg gaacgggcca gataggcaaa
      721 gcggttattg agcggctgcg aggatttgga tgtaaagtgt tggcttatag tcgcagccga
      781 agtatagagg taaactatgt accgtttgat gagttgctgc aaaatagcga tatcgttacg
      841 cttcatgtgc cgctcaatac ggatacgcac tatattatca gccacgaaca aatacagaga
      901 atgaagcaag gagcatttct tatcaatact gggcgcggtc cacttgtaga tacctatgag
      961 ttggttaaag cattagaaaa cgggaaactg ggcggtgccg cattggatgt attggaagga
     1021 gaggaagagt ttttctactc tgattgcacc caaaaaccaa ttgataatca atttttactt
     1081 aaacttcaaa gaatgcctaa cgtgataatc acaccgcata cggcctatta taccgagcaa
     1141 gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt tggattttga aaggagacag
     1201 gagcatgaat agaataaaag ttgcaatact gtttgggggt tgctcagagg agcatgacgt
     1261 atcggtaaaa tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt
     1321 atacattgga attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg
     1381 ggaaaacgac aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact
     1441 tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca
     1501 tggcaagtca ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt
     1561 tgtaggctgc gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt
     1621 tgcgaaaaat gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc
     1681 ggtggcagct acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt
     1741 cggtgtgaaa aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca
     1801 atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt
     1861 attgggaaac agtgccgcgt tagctgttgg cgaggtggac caaatcaggc tgcagtacgg
     1921 aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac
     1981 cgttcccgca gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat
     2041 atataaagcg ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg
     2101 ccgcattgta ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc
     2161 ccgtatgatg gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt
     2221 agcgttaaag gggtgataag catggaaata ggatttactt ttttagatga aatagtacac
     2281 ggtgttcgtt gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac
     2341 ggttatgaag taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca
     2401 aaagaactgg ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaat
     2461 cgtgctgtaa actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa
     2521 agttattatc ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa
     2581 tcaagccata gccgcggcag tgccattgat cttacgcttt atcgattaga cacgggtgag
     2641 cttgtaccaa tggggagccg atttgatttt atggatgaac gctctcatca tgcggcaaat
     2701 ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt
     2761 gggtttgaag catatagcct cgaatggtgg cactatgtat taagagacga accatacccc
     2821 aatagctatt ttgatttccc cgttaaataa acttttaacc gttgcacgga caaactatat
     2881 aagctaactc tttcgggttc tgttgcaaag ttttaaataa agaataaaat ccc
//
LOCUS       DACEKG010000223         1466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E391
            SAMN07141471-rid9870683.guided.184, whole genome shotgun sequence.
ACCESSION   DACEKG010000223 DACEKG010000000
VERSION     DACEKG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141471
            Sequence Read Archive: SRR5811541
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740871.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740871.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:48:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 34 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 10 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141471-rid9870683.guided.184"
                     /isolate="E391"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Mar-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1348
                     /locus_tag="IW664_002947"
     CDS             <1..1348
                     /locus_tag="IW664_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ0850219.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          622 a          117 c          246 g          481 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgt
//
